Title:
CHIMERIC INFECTIOUS DNA CLONES, CHIMERIC PORCINE CIRCOVIRUSES AND USES THEREOF
Document Type and Number:
WIPO Patent Application WO2003049703
Kind Code:
A3
Abstract:
The present invention relates to infectious DNA clones, infectious chimeric DNA clones of porcine circovirus (PCV), vaccines and means of protecting pigs against viral infection or postweaning multisystemic wasting syndrome (PMWS) caused by PCV2). The new chimeric infectious DNA clone and its derived, a virulent chimeric virus are constructed from the nonpathogenic PCV1 in which the immunogenic ORF gene of the pathogenic PCV2 replaces a gene of the nonpathogenic PCV1, preferably in the same position. The chimeric virus advantageously retains the nonpathogenic phenotype of PCV1 but elicits specific immune responses against the pathogenic PCV2. The invention further embraces the immunogenic polypeptide expression products.
Inventors:
MENG XIANG-JIN
FENAUX MARTIJN
HALBUR PATRICK G
FENAUX MARTIJN
HALBUR PATRICK G
Application Number:
PCT/US2002/039646
Publication Date:
September 30, 2004
Filing Date:
December 11, 2002
Export Citation:
Assignee:
VIRGINIA TECH INTELL PROP (US)
UNIV IOWA STATE RES FOUND INC (US)
UNIV IOWA STATE RES FOUND INC (US)
International Classes:
C12N15/09; A61K35/76; A61K38/00; A61K39/12; A61K48/00; A61P31/20; A61P43/00; C07K14/01; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N7/04; C12N15/11; C12N15/34; C12N15/63; C12P21/02; A61K39/00; A61K; (IPC1-7): A61K39/12; A61K39/295; A01N63/00; C12P19/34; C12N15/64; C12N7/00; C12N7/04; C12N5/00; C12N15/00; C07K17/00
Foreign References:
| US6217883B1 | 2001-04-17 | |||
| US6368601B1 | 2002-04-09 |
Other References:
NAWAGITGUL P. ET AL: "Open reading frame 2 of porcine circovirus type 2 encodes a major capsid protein", J. OF GENERAL VIROLOGY, vol. 81, no. 9, 2000, pages 2281 - 2287, XP002978778
MAHE D. ET AL: "Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes", J. OF GENERAL VIROLOGY, vol. 81, 2000, pages 1815 - 1824, XP002978779
LUSTIG S. ET AL: "Molecular basis of sindbis virus neurovirulence in mice", J. OF VIROLOGY, vol. 62, no. 7, July 1998 (1998-07-01), pages 2329 - 2336, XP000852560
CAGGANA M. ET AL: "Identification of a single amino acid residue in the capsid protein VP1 of coxsackievirus B4 that determines the virulent phenotype", J. OF VIROLOGY, vol. 67, no. 8, August 1993 (1993-08-01), pages 4797 - 4803, XP002979069
MAHE D. ET AL: "Differential recognition of ORF2 protein from type 1 and type 2 porcine circoviruses and identification of immunorelevant epitopes", J. OF GENERAL VIROLOGY, vol. 81, 2000, pages 1815 - 1824, XP002978779
LUSTIG S. ET AL: "Molecular basis of sindbis virus neurovirulence in mice", J. OF VIROLOGY, vol. 62, no. 7, July 1998 (1998-07-01), pages 2329 - 2336, XP000852560
CAGGANA M. ET AL: "Identification of a single amino acid residue in the capsid protein VP1 of coxsackievirus B4 that determines the virulent phenotype", J. OF VIROLOGY, vol. 67, no. 8, August 1993 (1993-08-01), pages 4797 - 4803, XP002979069
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