Title:
CIRCPOLK AS THERAPEUTIC TARGET AND DIAGNOSTIC BIOMARKER FOR TUMORS AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2023/155857
Kind Code:
A1
Abstract:
The present invention belongs to the technical field of tumor diagnosis. Disclosed in the present invention are circPOLK as a therapeutic target and diagnostic biomarker for tumors and the use thereof. The nucleotide sequence of circPOLK is as shown in SEQ ID NO: 10, and circPOLK is a circular RNA formed by means of the reverse splicing of the exons 1-3 of POLK mRNA. Compared with normal patients, circPOLK in the blood exosome in patients with tumors shows a high expression. The expression of circPOLK is positively correlated with the migration, invasion and metastasis capacity of lung cancer cells, and lung cancer cells can secrete circPOLK by means of exosomes to promote the migration and invasion capacity of vascular endothelial cells. Therefore, circPOLK can be used as a therapeutic target and diagnostic biomarker for tumors, providing a new school of thought and direction for the development of therapeutic drugs and diagnostic products.
Inventors:
ZHANG CHONG (CN)
LI YANGLING (CN)
CENG LINGHUI (CN)
LIU YEHAN (CN)
LING SHISHENG (CN)
DONG WENKUN (CN)
LI YANGLING (CN)
CENG LINGHUI (CN)
LIU YEHAN (CN)
LING SHISHENG (CN)
DONG WENKUN (CN)
Application Number:
PCT/CN2023/076595
Publication Date:
August 24, 2023
Filing Date:
February 16, 2023
Export Citation:
Assignee:
CITY COLLEGE OF ZHEJIANG UNIV (CN)
ASSURE TECH HANGZHOU CO LTD (CN)
ASSURE TECH HANGZHOU CO LTD (CN)
International Classes:
C12N15/11; A61P35/00; A61P35/04; C12N15/113; C12Q1/6886
Foreign References:
| CN114517204A | 2022-05-20 |
Other References:
ANONYMOUS: "CircRNA ID hsa_circ_0073052, Location chr5:74842834-74848416, Genomic Length 5582 bp, Spliced Seq Length 268 bp, Best Transcript NM_016218, Gene Symbol POLK", CIRCULAR RNA INTERACTOME-NIH, 1 January 2015 (2015-01-01), XP093086731, Retrieved from the Internet [retrieved on 20230928]
BAI, YUXI: "Analyze Gene Changes Characteristics of Multiple Synchronous Lung Adenocarcinomas through Targeted Sequencing", MASTER'S THESIS, 1 March 2021 (2021-03-01), CN, pages 1 - 58, XP009549187, DOI: 10.27162/d.cnki.gjlin.2021.005640
MICHIELS S., DANOY P., DESSEN P., BERA A., BOULET T., BOUCHARDY C., LATHROP M., SARASIN A., BENHAMOU S.: "Polymorphism discovery in 62 DNA repair genes and haplotype associations with risks for lung and head and neck cancers", CARCINOGENESIS, OXFORD UNIVERSITY PRESS, GB, vol. 28, no. 8, 7 March 2007 (2007-03-07), GB , pages 1731 - 1739, XP093086742, ISSN: 0143-3334, DOI: 10.1093/carcin/bgm111
YANQINGWANG, SEIMIYA MIKA, KAWAMURA KIYOKO, LINGYU, OGI TOMOO, TAKENAGA KEIZO, SHISHIKURA TOMOTANE, SAKLYAMA SHIGERU, TAGAWA MASAT: "Elevated expression of DNA polymerase K in human lung cancer is associated with p53 inactivation: Negative regulation of POLK promoter activity by p53", INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 25, no. 1, 1 January 2004 (2004-01-01), pages 161 - 165, XP093086749, DOI: 10.3892/ijo.25.1.161
CHEN, JINNA ET AL.: "Genetic Variations in miRNA Seed Regions and TLS Pathway Genes Associated with Survival in Small Cell Lung Cancer Patients Receiving Platinum-based Chemotherapy", ZHONGHUA ZHONGLIU ZAZHI (CHINESE JOURNAL OF ONCOLOGY), ZHONGGUO KEXUE YIXUE KEXUEYUAN, CN, vol. 41, no. 2, 23 February 2019 (2019-02-23), CN , pages 112 - 117, XP009549227, ISSN: 0253-3766
HUANG JIA-WEI, HUANG DAN, LI LI-XIA, YANG ZHI-XIONG: "Role of circRNA-miRNA-mRNA regulatory network in diagnosis, prognosis and therapeutic resistance of non-small cell lung cancer", JOURNAL OF GUANGDONG MEDICAL UNIVERSITY., vol. 39, no. 4, 1 August 2021 (2021-08-01), pages 494 - 498, XP093086753
BAI, YUXI: "Analyze Gene Changes Characteristics of Multiple Synchronous Lung Adenocarcinomas through Targeted Sequencing", MASTER'S THESIS, 1 March 2021 (2021-03-01), CN, pages 1 - 58, XP009549187, DOI: 10.27162/d.cnki.gjlin.2021.005640
MICHIELS S., DANOY P., DESSEN P., BERA A., BOULET T., BOUCHARDY C., LATHROP M., SARASIN A., BENHAMOU S.: "Polymorphism discovery in 62 DNA repair genes and haplotype associations with risks for lung and head and neck cancers", CARCINOGENESIS, OXFORD UNIVERSITY PRESS, GB, vol. 28, no. 8, 7 March 2007 (2007-03-07), GB , pages 1731 - 1739, XP093086742, ISSN: 0143-3334, DOI: 10.1093/carcin/bgm111
YANQINGWANG, SEIMIYA MIKA, KAWAMURA KIYOKO, LINGYU, OGI TOMOO, TAKENAGA KEIZO, SHISHIKURA TOMOTANE, SAKLYAMA SHIGERU, TAGAWA MASAT: "Elevated expression of DNA polymerase K in human lung cancer is associated with p53 inactivation: Negative regulation of POLK promoter activity by p53", INTERNATIONAL JOURNAL OF ONCOLOGY, vol. 25, no. 1, 1 January 2004 (2004-01-01), pages 161 - 165, XP093086749, DOI: 10.3892/ijo.25.1.161
CHEN, JINNA ET AL.: "Genetic Variations in miRNA Seed Regions and TLS Pathway Genes Associated with Survival in Small Cell Lung Cancer Patients Receiving Platinum-based Chemotherapy", ZHONGHUA ZHONGLIU ZAZHI (CHINESE JOURNAL OF ONCOLOGY), ZHONGGUO KEXUE YIXUE KEXUEYUAN, CN, vol. 41, no. 2, 23 February 2019 (2019-02-23), CN , pages 112 - 117, XP009549227, ISSN: 0253-3766
HUANG JIA-WEI, HUANG DAN, LI LI-XIA, YANG ZHI-XIONG: "Role of circRNA-miRNA-mRNA regulatory network in diagnosis, prognosis and therapeutic resistance of non-small cell lung cancer", JOURNAL OF GUANGDONG MEDICAL UNIVERSITY., vol. 39, no. 4, 1 August 2021 (2021-08-01), pages 494 - 498, XP093086753
Attorney, Agent or Firm:
HANGZHOU HUAZHI PATENT OFFICE (GENERAL PARTNERSHIP) (CN)
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