Title:
CLONED DNA ENCODING A UDP-GALNAC:POLYPEPTIDE,N-ACETYLGALACTOS AMINYLTRANSFERASE
Document Type and Number:
WIPO Patent Application WO1994026906
Kind Code:
A3
Abstract:
The present invention relates to a method for the isolation and expression of a glycosyltransferase enzyme for use in the synthesis of oligosaccharide or polysaccharide structures on glycoproteins, glycolipids, or as free molecules. The gene coding for the enzyme N-acetylgalactosaminyltransferase and the polypeptide sequence of the acceptor peptide for the enzyme N-acetylgalactosaminyltransferase have been isolated and used for the control of glycosylation of a protein.
Inventors:
ELHAMMER AKE P (US)
HOMA FRED L (US)
HOMA FRED L (US)
Application Number:
PCT/US1994/002552
Publication Date:
June 13, 1996
Filing Date:
March 17, 1994
Export Citation:
Assignee:
UPJOHN CO (US)
ELHAMMER AKE P (US)
HOMA FRED L (US)
ELHAMMER AKE P (US)
HOMA FRED L (US)
International Classes:
C07K7/06; C12N1/21; C12N5/10; C12N9/10; C12N15/54; C12P21/02; C12N15/09; C12R1/125; C12R1/19; (IPC1-7): C12N15/54; C12N9/10; C07K7/06
Domestic Patent References:
| WO1991012340A1 | 1991-08-22 | |||
| WO1991003484A1 | 1991-03-21 | |||
| WO1991006635A1 | 1991-05-16 |
Other References:
DATABASE EMBL 7 May 1993 (1993-05-07)
"Isolation and expression of a cDNA clone encoding a bovine UDP-GalNAcetylgalactosaminyltransferase", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 17, 15 June 1993 (1993-06-15), pages 12609 - 12616
ELHAMMER ET AL.: "The specificity of UDP-GalNAc: polypeptide N-actylgalactosaminyltransferase as inferred from a database of in-vivo substrates and from the in-vitro glycosylation of proteins and peptides", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 14, 15 May 1993 (1993-05-15), pages 10029 - 10038
O' CONNELL ET AL.: "The influence of flanking sequence on the O-glycosylation of threonine in-vitro", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 35, 15 December 1992 (1992-12-15), pages 25010 - 25018
"Isolation and expression of a cDNA clone encoding a bovine UDP-GalNAcetylgalactosaminyltransferase", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 17, 15 June 1993 (1993-06-15), pages 12609 - 12616
ELHAMMER ET AL.: "The specificity of UDP-GalNAc: polypeptide N-actylgalactosaminyltransferase as inferred from a database of in-vivo substrates and from the in-vitro glycosylation of proteins and peptides", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 268, no. 14, 15 May 1993 (1993-05-15), pages 10029 - 10038
O' CONNELL ET AL.: "The influence of flanking sequence on the O-glycosylation of threonine in-vitro", JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 267, no. 35, 15 December 1992 (1992-12-15), pages 25010 - 25018
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