This invention relates to cosmetic, health- and body-preserving compositions of high biological value, promoting the restora¬ tion of the cells and of that the skin, opti¬ mizing the biological processes occurring in the skin cells and providing the most prefer¬ able function of the enzyme system of the cells connected with the age of the organism.

The aim of the invention is to provide the restoration and preservation of the cells and the skin by using natural substances. Accord¬ ing to the invention, this aim is accomplished by treating proteins, suitably prepared from the blood or from the elements thereof with inorganic materials occurring as trace elements in the or¬ ganism, or with natural, river-, lake-, sea-, mi¬ neral and medicinal waters, with inorganic ate- rials obtained from these waters, by transforming the materials obtained to various cosmetic, health- and body-preserving compositions and by using those for serving these purposes.

It is known that the trace elements par- ticipating of the active transfer and being compo¬ nents of a number of various enzymes, exert a de¬ cisive influence on the funtion of the living or¬ ganism. Their deficiency, e.g. that of copper, i- ron or zinc leads to the degeneration of the cel¬ lular activity; thus the cell respiration, the hematopciesis, brain tissues, lungs and nervous system also become damaged. Although the primary ro e of a cosmetic appearing for a superficial viewer is the care of the skin, it can be statel

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that the harmonized function and health of the living organism and that of its parts should not be considered as a result of distinct effects, since all organs and tissues of the organism are in mutual interaction.

The disorders arising from the defi¬ ciency of trace elements exert a general influ¬ ence on the organism and hence also on the con¬ dition of the skin which is most striking for the observer. The skin is a highly differentiated or¬ gan and its condition has a decisive influence on the aesthetic appearance of a man.

In many cases the elimination of the disorders arising from the deficiency of the - trace elements is not a simple task since the ab¬ sorption, of the salts of trace elements, e.g. of iron sulphate in a form capable of taking a suffi¬ cient parir of the physiological processes is not satisfactory per se. According to the present knowledges, the trace elements are only capable of playing their role in the organism, in the skin cell3 when they can be coupled with a so-called carrier mole¬ cule transferring these elements to the site of action and thus providing the incorporation into the appropriate enzyme. Such carrier molecules are in general proteins, obtained particularly from the blood or from the blood plasma, respectively. The eicact mechanism of this process is not known at present, it is understood however, that the connection of the trace element with a protein has a decisive importance in a multi-step process.

Prom among the trace elements, the role of which is known, zinc, -copper, iron, cobalt, manganese, silicium and magnesium, etc. are essen-

tial for the skin. Thus, 20 per cent of the zinc content of the human organism are found in the skin. Zinc plays an important role in the pro¬ tein synthesis of the skin. Copper has a high importance in the structural development of the collagen and elastin, in addition to its well known effect on the blood count. '.Then these trace elements are not present in a sufficient amount, the solubility of the collagen and elas- tin is increased and their function is deficient. Manganese and magnesium take part in a number of enzyme reactions, e.g. of the collagen synthesis, however their effects on the activity of the en¬ zymes regulating the nucleic acid matebolism are also significant. Silicium plays an important role in the structural development of both the collage- nous and cartilaginous tissues. In addition to the trace elements mentioned above, a number of other ones, such as selenium as well as sulphur, sul- phides, vanadium and nickel also possess important roles. Practically all inorganic materials are sig¬ nificant for the optimum function of a living cell, which is not surprising while considering that all these materials were present in the primary ocean, at the appearance of the life.

It is known that an inorganic sub¬ stance, trace element or ion can get into the active transfer by being bound to a carrier protein on the one side /outer side/ of the cell .membrane -"here a carrier-substrate complex is formed. This complex penetrates the membrane, then the carrier protein leaves the substrate on the other side of the membrane and returns to the membrane surface. Such carrier molecules are the plasma proteins, tra s- ferrin,. alpha-microglobulins, transmangin, nicke-

loplasmins, etc. The number and nature of these substances are not exactly known at present, their existence and roles are, however, well demonstrated. There are cosmetics containing trace elements as well as other cosmetic compositions utilizing various proteins known for providing distinct types of activities, e.g. hydratating ability, etc. These agents, however, do not mean an optimum solution for the supply of the skin and that of the organism through the skin with trace element biocatalysts since the trace ele¬ ments cannot be absorbed advantageously from an aqueous medium either oer se /as such/ or in their common organic complexes. Thus skin or rheumatic disorders /diseases/ react with conflic¬ ting and widely scattering results to medicinal baths and mineral water therapy or are even inef¬ fective, though the balneologists might expect an unambiguous, preferable action of these baths and mineral waters on the basis of the quality and quantity of the inorganic salts of these waters. It is known that the compositions of medicinal and mineral waters used for decades or centuries show nearly no or a few differences; that of -the sea- -water can be considered as stable under natural conditions. In- spite of these facts the utility of these waters is varying or they are even in¬ active for patients suffering from frequently i- dentical skin disorders or diseases. As the com- oosi ion of mineral waters on i ^g mostly the trace elements needed to the skin and organism, is stable, the reasons for these extreme results should be found in the individual characteristics of the pati2nts. Likely, ^~ Λ'. reason for the di-

vergent results consists in that the carrier molecules transferring the trace elements are not always available. The trace elements, in¬ organic salts and ions as such cannot freely diffuse through the skin into the living or¬ ganism and the cells to a sufficient extent and cannot be bound to the enzymes; this occurs only by the aid of the carrier molecules. These carriers can specifically transfer only a single, defined trace element to the site of the- action and it can be supposed that these carriers are present in the blood.

Thus, the aim of this invention is to use the plasma proteins as a whole by trea- ting those with trace elements, mineral, medici¬ nal, natural waters and v/ith the materials there¬ of to obtain compositions possessing a satisfacto¬ ry activity.

It was found that compositions can be obtained 'which show an extraordinarily outstand¬ ing activity for the care of skin, for the therapy of rheumatic pains, for improvement of the hair quality, restoration of the hair bulbs as well as for the treatment of skin disorders and eczematous symptoms caused by detergents, by treating the proteins, e.g. prepared from the blood or blood elements v/ith the appropriate trace elements and optionally by heating the mixture obtained.

By treating the blood elements, e.g. plasma proteins v/ith microelements, mineral ar.i medicinal waters, vith inorganic materials obtained

ing effect may be prepared, such as that described e.g. i- Example 6. This composition was subjected

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- O - to animal experiments which are uncommon for the cosmetical industry but are suitable to evidence that the cosmetics of the invention have preferable and surprising activities. The results of these experiments are summarized in Table 1 below. The study was carried out as described by H. C. Stoerk et al. [Am. J. Path. 20 , 616 /1954/].

The action of the cream described in Example 6 was studied by using the adjuvant arthritis test on Long-Evans rats to observe a possible measurable effect of a cosmetic con¬ taining plasma proteins treated with medicinal water or with materials obtained therefrom on the alteration of the rat arthritis. For obtai¬ ning a better comparison, the rats under test were treated with a cream containing 1 or 5 per cent, respectively, of phenylbutazone known as one of the best anti-arthritic agents at present. The arthritis v/as induced by injecting "Ravenal" /ϊvlycobacterium tuberculosis in a concentration of 0.2 g/kg, in an amount of 0.1 ml/paw, in a pa¬ raffin oil suspension/ to the right posterior paw of the rat. The left posterior and both an- terior paws of the animals were treated v/ith the cream daily once for three weeks, i.e. lb times in the course of the experiment. The evaluation was made on the basis of the volume alteration of the left posterior paw. /A generalization of the arthritis v/as indicated by the swelling of the joints of the lets./ Z.i ^: ;he dα ^~ 21 in addi¬ tion to the volume altericn of the left posterior paw, the leg use and ic l state of the ani¬ mals, as compared to the e r.trcls, were also scored. /This gave an inf-r at on about the mo-

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bility of the arthritic rats./

Twelve rats were used for the investigation of each cream in our experiments. The averages of the experimental results are summarized in Table 1. It is clearly demonstra¬ ted from the results of Table 1 that the cream described in Example 6 had a stronger effect than the cream containing 1 per cent of phenyl- butazone, because it v/as as effective as a cream containing 5 per cent of phenyl utazone. The cream described in Example 6, though used daily once only, inhibited the degenerative alteration of the joints and improved the walking and motion. Both the physical condition and mobility of the animals were good. This test, while being not cosmetical in character, gives a satisfactory in¬ direct evidence for the surprising effect of the compostion of the invention in spite of the fact that its mechanism of action is clearly different from that of phenylbutazone. This test can be considered to have conclusive force from the point of view that plasma proteins treated v/ith micro¬ elements, mineral waters and/or v/ith inorganic ma¬ terials obtained therefrom play an outstanding role in the transfer to the skin cells and the organism of elements needed to the o tirt m conditions for • the skin cells as well as for physiological process¬ es. It is thought that this beneficial effect is due to the use of proteins as active ingredients prepared from the blood and treated v/ith trace elements, natural, mineral and medicinal watrr.t and/cr with inorganic materials obtained there¬ from in the cosmetic cometsiticas of the invent!:..

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The cosmetics prepared according to the invention are highly useful for the care and restoration of the skin, for the treatment of pustulous and inflammated skin and surprising- ly, for the alleviation and abolishment of rhe¬ umatic pains.

The compositions of the invention are also preferably be used for the treatment of psoriasis when the appropriate mediciπ.a.1 v/ater is chosen as an aqueous phase and mixed v/ith the protein component. Such medicinal water components are e.g. the waters cf Harkany or Hajduszoboszlό as described in Examples 1 and 3. By using the medicinal water of Harkany and Heviz - as descri- bed in Examples 20 and 22, respectively, the restoration of hair bulb3 is promoted and the hair fattening is diminished.

The composition described in Example 24 enhances the restoration of skin damaged by sun- burn and exerts a beneficial effect on milder household burn injuries.

The beneficial effect of the compositions of the invention is intensified v/hen in addition to or instead of medicinal waters, the mixtures of plant extracts and/or plant juices v/ith medicinal waters, the mixtures of natural waters and/or extracts and the solutions thereof enriched with the required trace elements are used as aqueous phases of the compositions .of the invention. ^' .Then the compositions of the invention are to be used for alleviating of mainly rheumatic pains, then the proteins are suitable treated v/ich ncble metal salts and with the solutions thereof

_*

rated by Zxa tle 2.

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The compositions and embodiments of the invention are illustrated by the following non- -li iting Examples. Example 1 A mixture containing 137.5 g of white petrolatum /vaseline/, 82.5 g of cetyl alcohol, 27.5 g of paraffin oil and 27.5 g of Tween 60 is heated to 80 °C to form the fatty phase of the composition. Meanwhile, 10 g of plasma pro- tein prepared from cattle blood plasma are swollen in 200 ml of the medicinal water of Harkany and the mixture is heat-treated at 112 °G for 90 mi¬ nutes. After the _# treatment v/ith the medicinal water, the protein is cooled to 60 °C and mixed to the fatty phase kept at 80 °C. After ho ogena- tion, a further amount of 600 ml of the medicinal water of Harkany heated to 70 °C are added to the hot mixture and the whole are stirred until cool. A cream is obtained which possesses an excellent hydratating, skin-restoring /preserving/ effect and surprisingly, when smeared on the painful part of the body, it alleviates or ceases the rheumatic pains.

Example 2 The process described in Example 1 is followed, except that 1 mg of gold /III/-trichlo¬ ride is added to -the plasma protein before or after the heat treatment. Example 3 The process described in Example 1 or 2 is followed, except that the medicinal water of Hajduszoboszlό is used as an aqueous phase. Example 4

The process described in Example 1 or 2 is followed, except that the medicinal v/ater of

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Parad is used as an aqueous phase. Example 5

The process described in Examples 1 to 4 is followed, except that the plasma protein is treated with a medicinal water,

200 ml of which contain 1 mg of gold/III/-tri- chloride, 0.5 rag of silver nitrate, 0.1 mg of chromic chloride, 2 mg of ferrous sulphate, 1 mg of manganous chloride, 1 mg of ammonium olybdate, 0.5 mg of cupric sulphate, 1 mg zinc chloride, 0.5 mg cobaltic chloride, 0.2 mg of boric acid and 0.2 mg of nickelous sulp¬ hate added before the heat treatment. Example 6 The process described in Example 1 is followed, except that the plasma protein is treated with 200 ml of the medicinal v/ater ari¬ sing from the Lukacs-bath /Budapest/ which con¬ tain 1 mg of ferrous sulphate, 1 mg of cupric sulphate, 0.5 mg of nickelous sulphate, 1 mg ^• of zinc sulphate and 0.5 mg of cobaltic chloride^ ^' added previously.

Example 7

The process described in Examples 1 to 6 is follov/ed, except that the geyser water of Karlsbad is used a3 the aqueous phase of the composition.

Example 3

The process described in Examples 1 to 7 is follov/ed, except that the natural water of Balaton is used as an aqueous phase. Example 9

The process described in Example 1 is follov/ed, except that the plasma protein is heat-treated v/ith 2CC ml of distilled water and

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then 600 ml of sea-water, e.g. of the water of the Aegean Sea is used for preparing the cream. Example 10

The process described in Examples 1 to 6 is follov/ed, except that in addition to

200 ml of medicinal water employed to treat the plasma protein, the natural water of the Danube river is used for preparing the cream. Example 11 The process described in Example 1 is followed, except that the plasma protein Is treated with the medicinal water In the presence of 2 mg of zinc sulphate. Example 12 The process described in Example 1 is followed, except that, instead of the medi¬ cinal v/ater a v/ater is used which, contains in 1 ml each 5 u of copper, 5 /U of iron, 10 u of zinc, 2 /U of cobalt, 1 /ug of nickel, 20 ug of magnesium and 20 U of calcium.

Example 1

The process described in Example 1 is follov/ed, except that carrot juice is used as an aqueous phase. Example 14-

The process described in Examples 1 to 6 is followed, except that a vine prepared from grape is used as an aqueous phase. Example 15 The process described in Examples 1 to 6 is follov/ed, except that apple vine neutra¬ lized by sodium hydrogen carbonate is used as an aqueous phase.

Example 16 The process described in Examples 1

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to 6 is followed, except that a fruit juice, e.g. apple juice is used as an aqueous phase. Example 17

80 ml of v/ater and 2 g of sodium hydrogen carbonate are added to 20 ml of swine blood, then the mixture is kept at 120 °C for 1 hour. The material obtained is filtered and 700 ml of the medicinal water of Harkany are added to the filtrate. The aqueous solution obtained is worked up to a-cream in a manner known in the art to give an excellent, hydratating and skin-restoring /skin-preserving/ cosmetic which is also useful for alleviating rheumatic pains. Example 18

2 g of sodium hydrogen carbonate, 50 ml of aqueous camomile extract and 600 ml of the medicinal water of Heviz are added to 100 ml of sheep blood plasma. The mixture is stirred at 20 °C for 1 hour, then it is worked up to a body-preserving cream in a manner known in the art.

Example 19

The process described in Example 1 is followed, except that, instead of the medi¬ cinal water of Harkany, an 1 : 1 : 1 mixture con¬ taining the medicinal waters of Harkany, Hajdu¬ szoboszlό and Heviz is used as an aqueous phase. Example 20 The plasma protein prepared from 10 g of swine blood is treated v/ith 20G ml of the medi- cinal v/ater of Heviz at 120 oC. for 1 hour. TO solution obtained, 300 ml of the natural medici¬ nal water of Heviz are added, the mixture is fille d to ampoules of 2 ml volume and sterilized

after sealing. This ampouled composition is suitable to the preservation of the skin by manual infriction or by iontophoresis. Example 21 2 mg of ferrous sulphate, 1 mg of manganous chloride, 1 mg of cupric sulphate, 1 mg of zinc sulphate and the extract prepared from 1 g of camomile with 20 ml of hot water are added to 100 ml -of native cattle blood plasma. The mixture is heated to 30 °C under intensive stirring to give a jelly-like materi¬ al v/ith a highly hydratating and skin-restoring /skin-preserving/ effect. Example 22 The process described in Example 20 or 21 is followed, except that instead of the medicinal water of Heviz, an 1 : 1 mixture con¬ taining the medicinal water of Harkany and Kajdu- szoboszlό is used. Example 23

To a plasma protein powder prepared from 10 g of cattle blood, 2CC ml of the medici¬ nal water of Harkany kept at 70 C are added and the mixture is heated at 112 °C for 90 minutes. After cooling, 70 ml of natural medicinal water of Harkany, 30 g of Alfozide KT-25, 30 g of Hala betaine, 570 g of sodium lauryl ether sulphate, 20 g of Alfid-D-11, 2 g of Alphasept (a preser¬ vative) , 10 g of polyethyleneglycol I.I-2C0 and 10 ml of 6 ;s ethanol are added to the mixture to give an excellent refreshing, skin-restoring foam bath which also promotes the restoration of the rheumatic parts of the body. Example 24- A lasma protein prepared from 10 g

of swine-blood is treated v/ith 2C0 ml of the medicinal water of Harkany at 120 °C for 1 hour, then 125 ml of the medicinal water of Harkany and 125 ml of the medicinal v/ater of the Lukacs-bath /Budapest/ are added to the solution obtained. This mixture is supple¬ mented with an appropriate preservative and used in spray form to treat the skin injuries caused by sunburn. Example 2-5

A plasma protein prepared from 10 g of rabbit blood is treated v/ith 200 ml of the medicinal water of Hajduszoboszlό at 112 °C for 90 minutes, then 300 ml of the medicinal water of 3alf and 50 ml of sea-water are added to the hot solution. Then 92 g of Alfozide KT-25, 75 g of 3ala betaine, 190 g of sodium lauryl ether sulphate, 10 g of Alfid-D-lI and 2 g of Alphasept are added to the mixture to give an outstanding hair shampoo promoting tho restoration of the hair bulbs and the steady hair growth. Example 26

A plasma protein powder prepared from 10 g of cattle blood is sv/ollen v/ith 2C0 ml of the medicinal water of Harkany while stirring and kept at 112 °C for 1 hour. To the solution obtained, 3CC ml of a medicinal water of Harkany are added which contain 0.2 mg of ferrous sulphate, 0.2 mg of zinc sulphate, 0.2 mg of cupric sulphate, 0.1 mg of nickelous sulphate and C.l mg of cobaltic chloride. The mixture obtained is filled to ampoules of 2 ml volume and sterilized by heat. Thi ^" composition excellently inhibits the fatte¬ ning of the hair bj- infriction into the hair and hair skin and promotes the restoration of the

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hair bulbs. The composition also inhibits the seborrhea of the hair.

Example 27

A plasma protein prepared from 10 g of swine blood is transformed to a solu¬ tion by treating v/ith 100 ml of the medicinal water of Hajduszoboszlό and 100 ml of the medi¬ cinal v/ater of Harkany at 120 °C. To this solu¬ tion, a melt containing 100 g of cetyl alcohol _τ 101 g of isopropyl myristate, 220 g of sorbitol of 70 , 60 g of cosmetic stearin, 140 g of cosmetic vaseline oil, 2 g of glutaraldεhyde, 39.2. g of cosmetic lanolin, 20 g of propyleneglycol and 3 g of ipagin M is added. The mixture is supplemented with 1100 ml of medicinal water of Hajduszoboszlό kept at 60 C and vith 5 g of potassium hydroxide and stirred until cool. This composition is excellently useful as a hand- -preserving balsam to restore the eczematous skin disorders caused by e.g. detergents.

Example 28

800 g of a soap powder is dissolved in 2 litres of distilled v/ater at 30 °C, there¬ after 200 g of sodium lauryl ether sulphate, a plasma protein solution prepared from a cattle blood of 5 & v/ith 200 ml of medicinal water of Harkany at 120 °C, as well as 500 ml of glycerol, 8 g of ITipagin and an odour are added and stirred until cool. An excellent cream soap is obtained which inhibits the damaging of the skin and is particularly suitable to keep the inflammatei skin in a clean state.

Example 29

142 g of stearin is saponified by 23.5 g of potassium hydroxide in 5CC ml of dis-

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tilled v/ater at 30 C, then a solution prepared from 10 g of plasma protein with 200 ml of the medicinal water of Heviz at 160 °C, as well as 100 g of sodium lauryl ether sulphate, 100 g of glycerol, 3 g of llipagin 11 preservative a- gent are added and stirred until cool. An out¬ standing cleaning agent is obtained which inhi¬ bits the damaging /injury/ and desiccation of the skin. Example 30

To a melt prepared from 25 g of cetyl alcohol, 12 g of vaseline, 2.5 g of Tv/een 60 and 5 g of glycerol at 72 C, a plasma pro¬ tein solution of 1 % prepared v/ith 6 ml of the medicinal water of Heviz at 120 °C as well as

5 ml of ethanol are added. The mixture is coloured and aromatized as desired to give a lip- -preserving agent which diminishes the desicca¬ tion of the lips. Example 31

A mixture containing 137.5 g of white petrolatum /vaseline/, 32.5 g of cetyl alcohol, 27.5 of paraffin oil and 27.5 g Tv/een ^' 60 is heated to 30 °C, 700 ml of a medicinal v/ater of Harkany kept at 60 °C are added and the mixture is stirred until cool to give an excellent hydratating cream which is useful for the preservation of the inflammated skin. Example 32 The process described in Example

31 is follov/ed, except that a mixture contai¬ ning 350 ml of the medicinal water of Harkany and the extract of 3 g of camomile prepared with 350 mi of hot water is used as an aqueous phase.

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ample 33

The process described in Example 31 or 32 is follov/ed, except that 1 mg of ferrous sulphate, 1 mg of zinc sulphate, 1 mg of cupric sulphate,- 0.5 mg of nickelous sulphate, 0.5 m of manganous sulphate and 0.2 mg of ammonium molybdate are dissolved in the aqueous phase before mixing.

Example 34 A salt mixture obtained by evaporating

0.5 g of the medicinal v/ater of Harkany is mixed with 200 ml of distilled water, the plasma protein prepared from 10 g of cattle blood plasma is added ^" and the mixture obtained is heat-treated at 121 °C for 2 hours. The material obtained is worked up to a cream according to the Example I.