Title:
COMPOSITIONS AND METHODS FOR DETERMINING CANINE GENDER
Document Type and Number:
WIPO Patent Application WO2004063390
Kind Code:
A3
Abstract:
The present invention provides methods to determine gender of a canine subject, that include contacting a nucleic acid sample from the canine subject with at least one probe or primer specific for canine amelogenin, and using the binding of the at least one probe or primer to detect a difference between the canine amelogenin gene on the Y chromosome and the canine amelogenin gene on the X chromosome, thereby determining gender of the canine subject. In certain aspects, gender of the canine subject is determined by contacting the nucleic acid sample with a primer pair that generate different sized amplification products depending on whether an X chromosome or a Y chromosome copy of the canine amelogenin gene is amplified. In certain aspects and embodiments disclosed herein, in addition to detecting binding of at least one probe or primer to a canine amelogenin gene, methods of the present invention include detecting binding of at least one probe or primer to a canine microsatellite locus, thus providing a genotyping and gender determination assay.
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Inventors:
FERRIE BONITA J M (US)
JOHNSTON ERIC V (US)
DENISE SUE K (US)
JOHNSTON ERIC V (US)
DENISE SUE K (US)
Application Number:
PCT/US2004/000523
Publication Date:
May 19, 2005
Filing Date:
January 09, 2004
Export Citation:
Assignee:
METAMORPHIX INC (US)
FERRIE BONITA J M (US)
JOHNSTON ERIC V (US)
DENISE SUE K (US)
FERRIE BONITA J M (US)
JOHNSTON ERIC V (US)
DENISE SUE K (US)
International Classes:
C07H21/04; C12Q1/68; C12Q; (IPC1-7): C07H21/04; C12Q1/68
Foreign References:
| US5474796A | 1995-12-12 |
Other References:
ASANO ET AL: "Studies on the Recovery of Genomic DNA and Functional Genes for Mammalian Pelt Specimens", ANIMAL SCIENCE, vol. 70, no. 10, 1999, pages J351 - J362, XP008046830
TACHI ET AL: "Successful Molecular Clongin and Nucleotide Sequence Determination of Parital Amelogenin", J. OF REPRODUCTION AND DEVELOPMENT, vol. 48, no. 6, 2002, pages 633 - 638, XP008046898
YUASA ET AL: "Amelogenin Expression in Canine Oral Tissues and Lesions", J. COMP. PATH., vol. 179, 1998, pages 15 - 25, XP008046795
DATABASE GENBANK [online] September 2002 (2002-09-01), "Studies on the recovery of genomic DNA and functional genes from mammalian pelt specimens, Canis familiaris amelx gene for amelogenin", XP002986026, accession no. NCBI Database accession no. AB080686
TACHI ET AL: "Successful Molecular Clongin and Nucleotide Sequence Determination of Parital Amelogenin", J. OF REPRODUCTION AND DEVELOPMENT, vol. 48, no. 6, 2002, pages 633 - 638, XP008046898
YUASA ET AL: "Amelogenin Expression in Canine Oral Tissues and Lesions", J. COMP. PATH., vol. 179, 1998, pages 15 - 25, XP008046795
DATABASE GENBANK [online] September 2002 (2002-09-01), "Studies on the recovery of genomic DNA and functional genes from mammalian pelt specimens, Canis familiaris amelx gene for amelogenin", XP002986026, accession no. NCBI Database accession no. AB080686
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