Title:
COMPOSITIONS AND METHODS FOR GENERATING EXPRESSION VECTORS THROUGH SITE-SPECIFIC RECOMBINATION
Document Type and Number:
WIPO Patent Application WO2002000875
Kind Code:
A3
Abstract:
Compositions, kits, and methods are provided for use in a recombinational cloning or subcloning methods for constructing expression vectors which comprise: ligating a library of double-stranded linear donor DNAs, where each member of the library includes a donor DNA sequence, with a double-stranded linear driver DNA which includes a promoter sequence and a donor recombination site to form a single circular donor DNA, the single circular donor DNA not including an origin of replication, where the donor DNA sequence is under the transcriptional control of the promoter; and contacting the circular donor DNA and a circular acceptor vector in the presence of a recombinase to form a single fused circular vector, the circular acceptor vector comprising an origin of replication and an acceptor recombination site capable of recombining with the circular donor DNA.
Inventors:
CLARK ROBIN (US)
Application Number:
PCT/US2001/019770
Publication Date:
May 02, 2002
Filing Date:
June 20, 2001
Export Citation:
Assignee:
PROTEMATION INC (US)
CLARK ROBIN (US)
CLARK ROBIN (US)
International Classes:
C12N15/64; C12N15/66; (IPC1-7): C12N15/10; C12N15/90; C12N15/64; C12N15/66
Domestic Patent References:
| WO1999021977A1 | 1999-05-06 | |||
| WO1996040724A1 | 1996-12-19 | |||
| WO2000012687A1 | 2000-03-09 | |||
| WO2000029000A1 | 2000-05-25 | |||
| WO1997047758A1 | 1997-12-18 | |||
| WO2001005961A1 | 2001-01-25 | |||
| WO2001031039A1 | 2001-05-03 | |||
| WO2000052027A1 | 2000-09-08 | |||
| WO2000063410A1 | 2000-10-26 |
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