Title:
CONTINUOUS SEPARATION METHOD
Document Type and Number:
WIPO Patent Application WO2002055538
Kind Code:
A3
Abstract:
The present invention relates to a method of separating a tagged protein product from a raw cell culture comprising the steps of providing a chromatography column com-prising an adsorption resin and a sample of cell culture, passing said sample over said column to capture tagged protein product to the resin, optionally washing the resin by passing a suitable liquid over the column, and eluting the protein product from the col-umn. During the whole processing, the sample, washing liquid and eluent are added at flow rates that maintain an essentially continuous flow throughout the column, and at least two samples are processed in parallel. The invention also relates to an apparatus especially suitable for performing a method of separating a tagged protein product from a raw cell culture.
Inventors:
PLESS THOMAS (SE)
Application Number:
PCT/EP2002/000175
Publication Date:
January 30, 2003
Filing Date:
January 10, 2002
Export Citation:
Assignee:
AMERSHAM BIOSCIENCES AB (SE)
PLESS THOMAS (SE)
PLESS THOMAS (SE)
International Classes:
B01D15/18; B01D15/20; C07K1/22; (IPC1-7): C07K1/22; B01D15/08
Domestic Patent References:
| WO1999065586A2 | 1999-12-23 | |||
| WO2001038083A1 | 2001-05-31 |
Other References:
NORONHA S ET AL.: "Use of Streamline chelating for capture and purification of poly-His-tagged recombinant proteins", BIOSEPARATION, vol. 8, 1999, pages 145 - 151, XP008009948
SCHUSTER M ET AL.: "Protein Expression Strategies for Identification of Novel Target Proteins", JOURNAL OF BIOMOLECULAR SCREENING, vol. 5, no. 2, April 2000 (2000-04-01), pages 89 - 97, XP008010011
"BioDirectory 2000", 2000, AMERSHAM PHARMACIA BIOTECH, XP002219447
DRAVELING C ET AL.: "SwellGel: An Affinity Chromatography Technology for High-Capacity and High-Throughput Purification of Recombinant-Tagged Proteins", PROTEIN EXPRESSION AND PURIFICATION, vol. 22, 14 June 2001 (2001-06-14), pages 359 - 366, XP002219446
SCHUSTER M ET AL.: "Protein Expression Strategies for Identification of Novel Target Proteins", JOURNAL OF BIOMOLECULAR SCREENING, vol. 5, no. 2, April 2000 (2000-04-01), pages 89 - 97, XP008010011
"BioDirectory 2000", 2000, AMERSHAM PHARMACIA BIOTECH, XP002219447
DRAVELING C ET AL.: "SwellGel: An Affinity Chromatography Technology for High-Capacity and High-Throughput Purification of Recombinant-Tagged Proteins", PROTEIN EXPRESSION AND PURIFICATION, vol. 22, 14 June 2001 (2001-06-14), pages 359 - 366, XP002219446
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