Title:
DETECTING MICROORGANISMS OF THE YERSINIA PESTIS/YERSINIA PSEUDOTUBERCULOSIS SPECIES
Document Type and Number:
WIPO Patent Application WO2002095066
Kind Code:
A3
Abstract:
Method and nucleic acids for the detection of microorganisms of the Yersinia pestis/Yersinia pseudotuberculosis species and/or differentiation between Yersinia pestis and Yersinia pseudotuberculosis in a sample. The invention aims to provide safe detection and differentiation methods for microorganisms of the Yersinia pestis/Yersinia pseudotuberculosis species. The method consists of several steps: (a) Bringing the sample into contact with a combination of at least two first nucleic acids (primers) which hybridize with an area of their microbial genome preserved in the species Yersinia pestis/Yersinia pseudotuberculosis or part thereof; (b) the amplification of the microbial genome or of a part of it to generate at least one amplification part; (c) bringing the amplification fragment of fragments obtained in step (b) into contact with at least one second nucleic acid (probe), which comprises a specific partial sequence of the amplificated genome or a part thereof for the speciesYersinia pestis/Yersinia pseudotuberculosis, wherein the second nucleic acid (probe) is specifically hybridized with at least one amplification fragment while forming at least one hybrid nucleic acid; (d) detecting the hybrid nucleic acid.
Inventors:
GRABOWSKI REINER (DE)
KOHLHAUSSEN SIMONE (DE)
BERGHOF KORNELIA (DE)
KOHLHAUSSEN SIMONE (DE)
BERGHOF KORNELIA (DE)
Application Number:
PCT/EP2002/005580
Publication Date:
December 04, 2003
Filing Date:
May 21, 2002
Export Citation:
Assignee:
BIOTECON DIAGNOSTICS GMBH (DE)
GRABOWSKI REINER (DE)
KOHLHAUSSEN SIMONE (DE)
BERGHOF KORNELIA (DE)
GRABOWSKI REINER (DE)
KOHLHAUSSEN SIMONE (DE)
BERGHOF KORNELIA (DE)
International Classes:
C12Q1/68; C12Q1/689; (IPC1-7): C12Q1/68; C12N15/11
Domestic Patent References:
| WO1997041257A1 | 1997-11-06 | |||
| WO1995011996A1 | 1995-05-04 | |||
| WO1999022023A2 | 1999-05-06 | |||
| WO1996008582A2 | 1996-03-21 | |||
| WO2000069897A2 | 2000-11-23 |
Foreign References:
| DE19945916A1 | 2001-04-05 | |||
| US5620847A | 1997-04-15 | |||
| US6001564A | 1999-12-14 |
Other References:
NORKINA OLGA V ET AL: "Development of a diagnostic test for Yersinia pestis by the polymerase chain reaction.", JOURNAL OF APPLIED BACTERIOLOGY, vol. 76, no. 3, 1994, pages 240 - 245, XP009003977, ISSN: 0021-8847
TREBESIUS K ET AL: "Development of rRNA targeted PCR and in situ hybridization with fluorescently labelled oligonucleotides for the detection of Yersinia species", JOURNAL OF CLINICAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 36, no. 9, September 1998 (1998-09-01), pages 2557 - 2564, XP002204235, ISSN: 0095-1137
WOUDENBERG T. M. ET AL.: "QUANTITATIVE PCR BY REAL TIME DETECTION", PROCEEDINGS OF THE SPIE, vol. 2680, 1996, pages 306 - 315, XP000197422
NEUBAUER H. ET AL.: "A combination of different polymerase chain reaction (PCR) assays for the presumptive identification of Yersinia pestis.", JOURNAL OF VETERINARY MEDICINE SERIES B, vol. 47, no. 8, October 2000 (2000-10-01), pages 573 - 580, XP009003968, ISSN: 0931-1793
DATABASE GENSEQ [online] 7 October 1999 (1999-10-07), GRIFFAIS R.: "PCR primer used to amplify an ORF of Chlamydia trachomatis.", XP002227316, Database accession no. AAZ05841
GIBELLO A. ET AL.: "Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 1, January 1999 (1999-01-01), pages 346 - 350, XP002227315, ISSN: 0099-2240
SCHEU P. ET AL.: "RAPID DETECTION OF LISTERIA MONOCYTOGENES BY PCR-ELISA", LETTERS IN APPLIED MICROBIOLOGY, vol. 29, no. 4, December 1999 (1999-12-01), pages 416 - 420, XP000892485
TREBESIUS K ET AL: "Development of rRNA targeted PCR and in situ hybridization with fluorescently labelled oligonucleotides for the detection of Yersinia species", JOURNAL OF CLINICAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 36, no. 9, September 1998 (1998-09-01), pages 2557 - 2564, XP002204235, ISSN: 0095-1137
WOUDENBERG T. M. ET AL.: "QUANTITATIVE PCR BY REAL TIME DETECTION", PROCEEDINGS OF THE SPIE, vol. 2680, 1996, pages 306 - 315, XP000197422
NEUBAUER H. ET AL.: "A combination of different polymerase chain reaction (PCR) assays for the presumptive identification of Yersinia pestis.", JOURNAL OF VETERINARY MEDICINE SERIES B, vol. 47, no. 8, October 2000 (2000-10-01), pages 573 - 580, XP009003968, ISSN: 0931-1793
DATABASE GENSEQ [online] 7 October 1999 (1999-10-07), GRIFFAIS R.: "PCR primer used to amplify an ORF of Chlamydia trachomatis.", XP002227316, Database accession no. AAZ05841
GIBELLO A. ET AL.: "Development of a PCR assay for detection of Yersinia ruckeri in tissues of inoculated and naturally infected trout.", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, vol. 65, no. 1, January 1999 (1999-01-01), pages 346 - 350, XP002227315, ISSN: 0099-2240
SCHEU P. ET AL.: "RAPID DETECTION OF LISTERIA MONOCYTOGENES BY PCR-ELISA", LETTERS IN APPLIED MICROBIOLOGY, vol. 29, no. 4, December 1999 (1999-12-01), pages 416 - 420, XP000892485
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