Title:
DIFFERENTIATION OF SPECIES WITHIN THE MYCOBACTERIUM TUBERCULOSIS COMPLEX
Document Type and Number:
WIPO Patent Application WO/2011/148269
Kind Code:
A3
Abstract:
The present invention relates to a multiplex in vitro nucleic acid amplification method for identifying a species of the Mycobacterium tuberculosis complex present in a sample. The method includes detecting the presence or absence of a plurality of nucleic acid molecule targets in the sample in one reaction, wherein at least one of the nucleic acid molecule targets is present in the genome of one or more, but not all, of the species of the Mycobacterium tuberculosis complex. The invention also includes kits containing primers or probes for conducting this method, nucleic acids useful for performing this method and diagnostic techniques using these nucleic acids.
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Inventors:
REDDINGTON KATE MARY (IE)
BARRY THOMAS GERARD (IE)
O'GRADY JUSTIN JOSEPH (IE)
SMITH TERENCE JAMES (IE)
BARRY THOMAS GERARD (IE)
O'GRADY JUSTIN JOSEPH (IE)
SMITH TERENCE JAMES (IE)
Application Number:
PCT/IB2011/001719
Publication Date:
May 18, 2012
Filing Date:
May 25, 2011
Export Citation:
Assignee:
NAT UNIV IRELAND (IE)
REDDINGTON KATE MARY (IE)
BARRY THOMAS GERARD (IE)
O'GRADY JUSTIN JOSEPH (IE)
SMITH TERENCE JAMES (IE)
REDDINGTON KATE MARY (IE)
BARRY THOMAS GERARD (IE)
O'GRADY JUSTIN JOSEPH (IE)
SMITH TERENCE JAMES (IE)
International Classes:
C12Q1/68
Other References:
DATABASE EMBL [online] 29 August 2000 (2000-08-29), "Mycobacterium tuberculosis strain H2255 (W) insertion sequence IS6110, partial sequence; and flanking insertion site 21-9.", XP002663004, retrieved from EBI accession no. EM_PRO:AF228674 Database accession no. AF228674
DATABASE Geneseq [online] 30 March 2001 (2001-03-30), "IGFBP2 oligonucleotide #89.", XP002663005, retrieved from EBI accession no. GSN:AAF45250 Database accession no. AAF45250
WARREN R M ET AL: "Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference.", THE INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE : THE OFFICIAL JOURNAL OF THE INTERNATIONAL UNION AGAINST TUBERCULOSIS AND LUNG DISEASE JUL 2006 LNKD- PUBMED:16850559, vol. 10, no. 7, July 2006 (2006-07-01), pages 818 - 822, XP002663006, ISSN: 1027-3719
HERRERA-LEÓN LAURA ET AL: "Aplicación de métodos moleculares para la identificación de las especies del complejo Mycobacterium tuberculosis [Differentiation of species within the Mycobacterium tuberculosis complex by molecular techniques]", ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA, DOYMA, BARCELONA, ES, vol. 27, no. 9, 1 November 2009 (2009-11-01), pages 496 - 502, XP008144998, ISSN: 0213-005X, [retrieved on 20090501], DOI: 10.1016/J.EIMC.2009.01.008
B. A. PINSKY ET AL: "Multiplex Real-Time PCR Assay for Rapid Identification of Mycobacterium tuberculosis Complex Members to the Species Level", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 46, no. 7, 1 July 2008 (2008-07-01), pages 2241 - 2246, XP055011475, ISSN: 0095-1137, DOI: 10.1128/JCM.00347-08
POUNDER J I ET AL: "Mycobacterium tuberculosis complex differentiation by genomic deletion patterns with multiplex polymerase chain reaction and melting analysis", DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASES, ELSEVIER SCIENCE PUBLISHING CO., AMSTERDAM, NL, vol. 67, no. 1, 12 March 2010 (2010-03-12), pages 101 - 105, XP026995896, ISSN: 0732-8893, [retrieved on 20100409]
K. REDDINGTON ET AL: "Novel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 49, no. 2, 1 February 2011 (2011-02-01), pages 651 - 657, XP055011278, ISSN: 0095-1137, DOI: 10.1128/JCM.01426-10
DATABASE Geneseq [online] 30 March 2001 (2001-03-30), "IGFBP2 oligonucleotide #89.", XP002663005, retrieved from EBI accession no. GSN:AAF45250 Database accession no. AAF45250
WARREN R M ET AL: "Differentiation of Mycobacterium tuberculosis complex by PCR amplification of genomic regions of difference.", THE INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE : THE OFFICIAL JOURNAL OF THE INTERNATIONAL UNION AGAINST TUBERCULOSIS AND LUNG DISEASE JUL 2006 LNKD- PUBMED:16850559, vol. 10, no. 7, July 2006 (2006-07-01), pages 818 - 822, XP002663006, ISSN: 1027-3719
HERRERA-LEÓN LAURA ET AL: "Aplicación de métodos moleculares para la identificación de las especies del complejo Mycobacterium tuberculosis [Differentiation of species within the Mycobacterium tuberculosis complex by molecular techniques]", ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA, DOYMA, BARCELONA, ES, vol. 27, no. 9, 1 November 2009 (2009-11-01), pages 496 - 502, XP008144998, ISSN: 0213-005X, [retrieved on 20090501], DOI: 10.1016/J.EIMC.2009.01.008
B. A. PINSKY ET AL: "Multiplex Real-Time PCR Assay for Rapid Identification of Mycobacterium tuberculosis Complex Members to the Species Level", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 46, no. 7, 1 July 2008 (2008-07-01), pages 2241 - 2246, XP055011475, ISSN: 0095-1137, DOI: 10.1128/JCM.00347-08
POUNDER J I ET AL: "Mycobacterium tuberculosis complex differentiation by genomic deletion patterns with multiplex polymerase chain reaction and melting analysis", DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASES, ELSEVIER SCIENCE PUBLISHING CO., AMSTERDAM, NL, vol. 67, no. 1, 12 March 2010 (2010-03-12), pages 101 - 105, XP026995896, ISSN: 0732-8893, [retrieved on 20100409]
K. REDDINGTON ET AL: "Novel Multiplex Real-Time PCR Diagnostic Assay for Identification and Differentiation of Mycobacterium tuberculosis, Mycobacterium canettii, and Mycobacterium tuberculosis Complex Strains", JOURNAL OF CLINICAL MICROBIOLOGY, vol. 49, no. 2, 1 February 2011 (2011-02-01), pages 651 - 657, XP055011278, ISSN: 0095-1137, DOI: 10.1128/JCM.01426-10
Attorney, Agent or Firm:
GOODFELLOW, Hugh, Robin (One Southampton Row, London WC1B 5HA, GB)
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