Title:
DNA ENCODING HUMAN ALPHA AND BETA SUBUNITS OF NEURONAL NICOTINIC ACETYLCHOLINE RECEPTOR
Document Type and Number:
WIPO Patent Application WO2002059266
Kind Code:
A3
Abstract:
Isolated nucleic acid molecules, i.e., DNA or RNA encoding human neuronal nicotinic acetylcholine receptor alpha and beta subunits, mammalian and amphibian cells containing said DNA, methods for producing alpha and beta subunits and recombinant (i.e., isolated or substantially pure) alpha subunits and beta subunits are provided. In addition, cells expressing various multimeric combinations of subunits (i.e., alpha1 alpha2 alpha3 alpha4 alpha5 alpha6 and/or alpha7 in combination with at least one of an alpha and beta subunit are also provided. A recombinant, non-human cell line expressing the human alpha7 subunit of nAChR is also disclosed.
Inventors:
GILLESPIE ALISON (US)
CLAEPS BRIAN O (US)
CHAVEZ-NORIEGA LAURA ELENA (US)
SIEGEL ROBERT (US)
ELLIOTT KATHRYN J (US)
CLAEPS BRIAN O (US)
CHAVEZ-NORIEGA LAURA ELENA (US)
SIEGEL ROBERT (US)
ELLIOTT KATHRYN J (US)
Application Number:
PCT/US2001/050985
Publication Date:
November 06, 2003
Filing Date:
October 29, 2001
Export Citation:
Assignee:
MERCK & CO INC (US)
GILLESPIE ALISON (US)
CLAEPS BRIAN O (US)
CHAVEZ-NORIEGA LAURA ELENA (US)
SIEGEL ROBERT (US)
ELLIOTT KATHRYN J (US)
GILLESPIE ALISON (US)
CLAEPS BRIAN O (US)
CHAVEZ-NORIEGA LAURA ELENA (US)
SIEGEL ROBERT (US)
ELLIOTT KATHRYN J (US)
International Classes:
C07K14/705; C12N1/15; C12N1/19; C12N1/20; C12N1/21; C12N15/09; C12N5/10; (IPC1-7): C07K1/00; C07H21/04; C12N1/20; C12P21/06; G01N33/566
Other References:
PENG X. ET AL.: "Human alpha-7 acetylcholine receptor: cloning of the alpha-7 subunit from the SH-SY5Y cell line and determination of pharmacological properties of native receptors and functional alpha-7 homomers expressed in xenopus oocytes", MOLECULAR PHARMACOLOGY, vol. 45, 1994, pages 546 - 554, XP002076578
QUIK M. ET AL.: "Similarity between rat brain nicotinic alpha-bungarotoxin receptors and stably expressed alpha-bungarotoxin binding sites", JOURNAL OF NEUROCHEMISTRY, vol. 67, no. 1, 1996, pages 145 - 154, XP002967512
QUIK M. ET AL.: "Similarity between rat brain nicotinic alpha-bungarotoxin receptors and stably expressed alpha-bungarotoxin binding sites", JOURNAL OF NEUROCHEMISTRY, vol. 67, no. 1, 1996, pages 145 - 154, XP002967512
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