Title:
DNA RECOMBINATION IN EUKARYOTIC CELLS BY THE BACTERIOPHAGE PHIC31 RECOMBINATION SYSTEM
Document Type and Number:
WIPO Patent Application WO2001007572
Kind Code:
A3
Abstract:
This invention provides methods for obtaining specific and stable integration of nucleic acids into eukaryotic cells. The invention makes use of site-specific recombination systems that use prokaryotic recombinase polypeptides, such as the PHI C31 integrase, that can mediate recombination between the recombination sites, but not between hybrid recombination sites that are formed upon the recombination. Thus, the recombination is irreversible in the absence of additional factors. Eukaryotic cells that contain the recombinase polypeptides, or genes that encode the recombinases, are also provided.
Inventors:
OW DAVID W
CALENDAR RICHARD
THOMASON LYNN
CALENDAR RICHARD
THOMASON LYNN
Application Number:
PCT/US2000/019983
Publication Date:
April 19, 2001
Filing Date:
July 21, 2000
Export Citation:
Assignee:
UNIV CALIFORNIA (US)
US AGRICULTURE (US)
US AGRICULTURE (US)
International Classes:
C12N1/15; C12N1/19; C12N5/10; C12N15/81; C12N15/09; C12N15/82; C12N15/85; C12N15/90; (IPC1-7): C12N5/10; C12N15/79; C12N15/90
Domestic Patent References:
| WO2000011155A1 | 2000-03-02 | |||
| WO1999018222A1 | 1999-04-15 |
Other References:
THORPE, H. M. ET AL.: "In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family", PNAS, vol. 95, 1998, pages 5505 - 5510, XP002157128
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