2. BRIEF DESCRIPTION OF PRIOR ART Products, such as medical supplies are sterilized to kill living organisms to an acceptable level. Direct testing for sterility is destructive and expensive and hence indirect testing methods are used. Biological indicators made from cultures, such as Bacillus subtils spores, bacillus pumilus spores and clostridium sporogenes spores are used for monitoring the sterilization. However, chemical indicators are widely used because they are simple and inexpensive.

A wide variety of medical supplies are sterilized with materials and techniques, such as steam, plasma, high energy radiation and ethylene oxide. Ethylene oxide is abbreviated herein as ETO. It is essential to assure that the medical supply is sterilized. A number of indicators, dosimeters and monitors are proposed in the literature. They include biological and chemical indicators. The color changing chemical indicators are inexpensive and hence are widely used. number of patents have been issued on ethylene oxide indicators.

U. S. Pat. No. 3,852,034 describes an indicator for ethylene oxide which includes an amino substituted indicating compound, e. g., acid salts of amino substituted triphenylmethanes, diphenylmethanes, azines or xanthenes, which undergoes color change based on replacement of labile hydrogen in amino groups with hydroxyethyl and a buffering agent selected to provide an ionic dissociation equilibrium such that color change occurs only when sterilization has been effective.

An indicator composition for ethylene oxide gas comprising 4- (4-nitrobenzyl) pyridine, nitrocellulose, a basic substance, and, optionally, a blue coloring agent is disclosed in U. S.

Pat. No. 4,094,642. The indicator composition, when exposed to ethylene oxide gas,

changes its color to develop a stable color indication, typically of a green color. The composition can take any shape and form, and is typically applied to a substrate, such as paper to form a layer thereon. Devices using 4- (4-nitrobenzyl) pyridine are described in U. S.

Pat. No. 4,678,640, U. S. Pat. No. and U. S. Pat. No. 4,436,819. For example U. S.

Pat. No. 4,436,819 describes a device for colorimetrically quantifying exposure to ethylene oxide which comprises a polyrner substrate which exhibits transport for ethylene oxide through the body of which is dispersed a concentration of a color-forming compound which undergoes a color-change upon reaction with ethylene oxide, said concentration being chosen to provide a variable degree of color-change in said device which, at a uniform temperature, is a previously determined function of the device's quantity of exposure to ethylene oxide. The color forming compound comprises a material selected from the group of ethylene oxide-reactive color formers consisting of 4- (p-nitrobenzyl) pyridine, N- phenylbenzylpyridine and phenazine and the basic catalysts is a member selected from the group consisting of triethanolamine, triethylenediamine, triethylenetetramine, N, N- bis (aminopropyl)-1,3-propanediarnine, and N, N, N', N'-tetrakis (methyl)-ethylenediamine.

An indicating composition, which undergoes a color change, that is progressive with the conditions and periods of sterilization, such that a final and cornplete color change indicates the completion of effective ethylene oxide sterilization is disclosed in U. S. Pat. No.

4,407,960. The indicating composition comprises a leuco precursor of an aryl methane dye selected from the groups of dyes such as Michler's hydrol, crystal violet lactone, malachite green leuco and crystal violet leuco; and an acidic constituent, such as diphenolic acid (4,4- bis [4-hydroxyphenyl] pentanoic acid).

A device for giving a visual indication of the amount of residual ethylene oxide present in ethylene oxide treated solid hospital articles and other health devices is disclosed in U. S. Pat. No. The amount of residual ethylene oxide is indicated by the difference in color exhibited by different sensitized areas of the device in the form of an opaque dark colored support member having bonded to its surface a thin film of a cholesteric liquid crystal composition in a solid film-forming binder.

An ink composition for indicating the progress of sterilization with ethylene oxide is provided in U. S. Pat. No. 5,258,065 which comprises; (1) at least one disperse dye of the general formula A-N=N-B wherein A is a residue of a heterocyclic compound containing nitrogen atom which is not substituted with alkyl group and selected from the group consisting of the pyridine, quinoline, isoquinoline, triazole, tetrazole, indazole, thiazole, benzothiazole and thiadiazole rings, which residue may optionally have one or more undissociated substituents, and B is a coupling component, (2) at least one binder component selected from the group consisting of polyacrylic acid, polymethacrylic acid and acrylic acid-methacrylic acid copolymers, (3) at least one ultrafine filler selected from the

group consisting of ultrafine particles of silica, aluminum oxide and titanium oxide, and (4) at least one polar solvent.

An ink composition has been prepared as a telltale for ethylene oxide sterilization, which utilizes the fact that magnesium chloride reacts with ethylene oxide to produce a base, magnesium hydroxide, which is detected by a pH sensitive dye; see U. S. Pat. No. 3,098,751.

A further development in this area of ethylene oxide monitoring is disclosed in U. S. Pat. No.

4,138,216. The device disclosed comprises a wick impregnated with magnesium chloride and a pH sensitive dye is enclose in a gas impervious envelope having one end open. An additional constituent is an acidic material, e. g., tartaric acid, which acts as a quantifier to adjust the time response of the device. This latter device is particularly useful in ethylene oxide sterilization monitoring because it is responsive to humidity levels as well as temperature and gas concentration. U. S. Pat. No. 5,451,372 discloses a device for monitoring an ethylene oxide sterilization process in which 100% ethylene oxide is utilized as the sterilant comprising a wick impregnated with an ethylene oxide responsive chemical compound a quantifier and a pH sensitive dye; an ethylene oxide impervious backing strip upon which the wick is rnounted; and a cover strip having an ethylene oxide impervious film.

The backing and cover strip are adhered to one another, and in intimate contact with and sealed to the wick. The ethylene oxide responsive chemical compound is a chloride of divalent metals such as magnesium, iron and zinc and the pH sensitive dye is bromophenol blue, thymol blue or xylenol blue.

Even though the production of a base upon exposure to ethylene oxide is reported in U. S. Pat. No. 3,098,751,4,138,216 and 5451372, it is always limited to chlorides of divalent metals, such as rnagnesium, iron and zinc. There is no report on use of halides of (1) rnono- valent rnetals, such as sodium and potassium, (2) the other halides, such as bromides or iodides, of di or higher valent rnetals, (3) organic halides, such as tetrabutylammoniurn bromides, for monitoring ethylene oxide and (4) other salts, both organic and inorganic, such as sodium thiocyanate. We have accidentally discovered that a coating composed of a monovalent organic or inorganic salt, such as sodium bromide, sodium thiocyanate and tetraethylammonium bromide, a pH sensitive dye, such as bromophenol blue, and a polymeric binder, such as a polyacrylate, undergoes a color change when exposed to ethylene oxide.

Sterilization with ETO depends upon several factors, such as time, temperature, humidity and concentration of ETO. In order to assure the sterilization with ETO, the indicator must determine integral value of these. It is also desirable that the indicator, in the absence of ethylene oxide, is essenlially unaffected by other parameters, such as dry heat, steam, radiation and ambient conditions.

SUMMARY OF THE INVENTION There is provided a polymer device for monitoring ethylene oxide as well as the presence of other epoxides comprising: at least one layer of polymer, having incorporated therein a) an indicator capable of undergoing at least one color change b) an activator for said indicator wherein said activator, when contacted with ethylene oxide, introduces a reaction wherein the product of said reaction causes said indicator to undergo said color change, provided said activator has a monovalent cation.

Such a device is made by coating a mixture of (a) a polymer as binder, (b) a ETO reactive salt having a monovalent cation and (c) a pH sensitive dye, when contacted with ETO, undergoes at least one color change. Such a device can be used for monitoring sterilization of medical supplies.

The formulations and devices disclosed herein offer several advantages over those based on magnesium chloride reported in U. S. Pat. No. 3,098,751,4,138,216 and 5451372.

The hydroxides of higher valent metals, such as magnesium hydroxide, are significantly weaker than those of alkali metals, such as potassium hydroxide and sodium hydroxide produced upon exposure to ethylene oxide. As a result, the device made from alkali metal and organic halides are significantly more sensitive than those made from magnesium chloride. Lower concentration of ethylene oxide can be detected with the devices disclosed herein. For example, a paper impregnated with sodium iodide or tetrabutylammonium bromide and bromothymol blue changes from yellow-to-blue almost instantly when exposed to 100% ethylene oxide or in minutes at about 20ppm of ethylene oxide. Hence, using the formulations proposed herein, one can make more sensitive devices similar to those proposed in U. S. Pat. No. 4,138,216 and 5451372. As monovalent halides, such as sodium iodide and potassium bromide are generally less sensitive to humidity than those of higher valent rnetals, the device disclosed herein is more stable to environmental humidity and reasonably sensitive to a mixture of humidity and ethylene oxide compared to those made from magnesium chloride and calcium chloride. The change in pH upon exposure to ETO is higher in case of iiiono-valetit halides and hence the color change is more dramatic and a larger number of pH sensitive dyes can be used to get a variety of color changes upon exposure to ethylene oxide. We have also found that di and higher valent metal halides cause precipitation of aqueous inks vehicle/binders, which are made water soluble or dispersible by incorporating acidic functionalities. Hence, it is very difficult to incorporate divalent rnetal salts such as magnesium chloride without causing the precipitation of most of the aqueous vehicles. The mollo-valent salts usually have lower toxicity and prices compared to those of the di-valent metal salts.

The indicators suitable for use in this device include pigments, dyes, precursors of said dyes, and mixtures of any of these. A desirable quality of the indicator is the ability to undergo a color change with the reaction product of ethylene oxide and said activator.

Desirably the indicator undergoes a yellow-to-blue, yellow-to-green, red-to-yellow red-to- green or red-to-blue color change.

The polyrner used in the device is, suitably, soluble in water or dispersible in an aqueous rnedium solvent. A broad class of polymers may be used. They may be homopolymers, copolymers or a mixture thereof.

Suitably the reaction product of the activator and ethyiene oxide is a base. Suitable activators are salts of monovalent cations, such as halides and isocyanates, preferably bromides. The activator may also be a salt of an amine and an organic or inorganic acid.

The device may additionally comprise an additive, such as an acid or base, to control the rate of reaction and color change.

The device may have two layers, that is to say additionally comprising a polymeric top layer. This may be a wedge shaped polymeric top layer.

The process of making a device of the present invention comprises dissolving the cornporlents thereof in a solvent thereof, applying the thus formed solution to a substrate and permitting the solvent to evaporate.

The substrate may be a container for an item to be sterilized. It may also be a plastic film, paper or metal, including but not limited polyester film, paper or spun bonded polyolefins.

In a desirable embodiment of the invention the solution is an ink formulation suitably an aqueous ink formulation most suitably one which comprises an acrylate polymer.

A process of using a device of the present invention for monitoring sterilization of materials comprises the steps of affixing the device to said materials or containers containing same, carrying out the process of sterilization including the step of exposing the device to ethylene oxide and observing the presence of a color change of said device.

BRIEF DESCRIPTION OF THE DRAWINGS Figure 1. A side schematic cross section of one embodiment of the ETO sterilization indicator of the invention where an indicator layer comprised of a polymeric binder, ETO activator and ETO indicator is applied on a substrate.

Figure 2. A side schematic cross section of the ETO sterilization indicator of the invention having an adhesive layer and a release layer.

Figure 3. A side cross-section of a multi-layer device wherein a top layer is a coating or lamination as a barrier.

BRIEF DESCRIPTION OF THE INVENTION AND PREFERRED EMBODIMENTS The device can be best described by reference to the Figures. As shown in Figure 1, the device in one of the simplest form is comprised of an indicator layer Q applied on a substrate 10. The substrate 10 can also be a container, such as pouch for products to be sterilized. The indicator layer 20 is composed of a polymeric binder 21, containing at least one ETO indicator, such as a pH sensitive dye 22, capable of undergoing a color change with a reaction product such as a base, produced by reaction of ETO with the ETO activator 23, when contacted with ETO. The coating 20 may contain other additives, e. g., additive 24 to control the rate of the color change and additive 25 to rnaintain the colors and shelf life.

As shown in Figure 2, the substrate 10 of the device can be coated with an adhesive layer 30. The adhesive layer allows the device to be affixed to a container of product to be sterilized. To the bottorn of the adhesive layer 30, can be affixed a release layer 40 for ease in packaging and for rernoval just prior to use. Removal of the release layer 40 will enable the entire device to he affixed to the container of product to be sterilized.

The device can be composed of more than one layer. In its simplest form, the device could have two layers. As shown in Figure 3, in its simplest form of the two-layer device, the second top layer 50 could be a barrier for ETO, e. g., a polymeric coat or a larninated film, on to layer 20. The barrier layer 50 can reduce diffusion of ETO, thereby increasing the time required for the color change.

A rnoving boundary device can be created if the barrier layer 50 is in form a wedge over the indicator layer 20. The barrier layer will resist but will be permeable to ethylene oxide.

Other variations of the ETO indicator device are also possible, for example, a gradient device can be created by coating a series of formulations having the time required for the color change either increases or decreases. Such gradient can be obtained by coating such formulations in form of lines or bars next to each other.

The device can also be created by printing the indicating formulation in form of a number, irnage, bar code or message, e. g.,"if this print is green, the product inside is sterilized".

The the invention has been practiced by using an acrylate printing ink extender 001270 supplied by Environmental inks and Coating, Co, Lithium, MD as a binder and tetraethylammoniurn brornide as an ETO activator and bromocresol purple as an indicator.

The acrylate ink extender 001270 is referred herein to as EC001270 and tetraethyiammonium bromkte as TEAB.

Classes of dyes: A wicle variety of dyes which change color with change in pH can be used as indicators, such as nitroso, nitro, azo (mono, di, tri and polyazo), azoic, stilbene, carotenoid, diphenyimethane, triphenylmethane, xanthene, acridine, quinoline, methane and polymeihine, thiazole, indamine and indophenol, azine, oxazine, sulfur, lactone, aminoketone, hydroxyketone, anthraquinone, indigoid, phthalocyanine, and natural.

A dye having an amine functionality and neutralized with acids, such as HI and HBr can undergo color change with ETO without activator or would require lower concentration of activator.

Concentration ot the activator required for the noticeable color change depends upon several factors, such as thickness of the coating and absorbance coefficient of the dyes.

Preferred concentration is 0.1 to 20% of the total solid of the coating. The most preferred range of the indicator concentration is 0.5 to 5%.

A large number of dyes, as listed in Table 1, were added in a mixture of EC001270 (1) TEAB (2) sodium thiocyanate and (3) tetrabutylphosphonium bromide and coated on paper and polyester film. Pieces of the coatings were exposed to ETO.

Table 1. List of dyes tested with TEAB, sodium thiocyanate and tetrabutylphosphonium brornide as ETO activator in EC001270.

Acid alizarin violet N, acid black 24, acid black 48, acid blue 113, acid blue 120, acl blue 129, acid bllJe 161, acid blue 25, acid blue 29, acid blue 40, acid blue 41, acid blue 4b, acid blue 80, acid blue 93, acid fuschin, acid green 25, acid green 27, acid green 41, acid orange

74, acid red 1, acid red 114. acid red 151, acid red 88, acid violet 17, acid violet 7, acid yellow 99, acridine orange, acridine orange base, acridine orange G, acridine yellow G, acriflavine hydrochloride, alcian blue 8GX, alcian yellow, alizarin, alizarin blue black SN, alizarin cornplexone, alizarin complexone dihydrate, alizarin red, alizarin violet 3R, alizarin yellow GG, alizarin yellow R, alkali blue 6B, alkali fast green 10GA, alphazurine A, aluminon, aminoacridine hydrochloride, aminoanthraquinone, aminophthalhydrazide, aniline blue, astra blue 6GLL, auramine O, azocarmine, azocarmine B, azure A, azure B, azure B thiocyanate, azure C, basic blue 3, basic blue 41, basic blue 66, basic fuchsin, basic red 29, basic yellow 11, benzo purpurin 4B, biebrich scarlet NA salt, bismarck brown B, bismarck brown Y, blue tetrazolium, bordeaux R, brillant blue B, brillant blue G, brilliant cresyl blue ALD, brillant crocein MOO, brillant green, brilliant sulphaflavine, brilliant yellow, bromochlorophenol blue, brornocresol green, bromocresol purple, bromophenol blue, bromopyrogallol red, bromothymot blue, bromoxytenot blue, calmagite, carbol fuchsin, carminic acid, carotene, celestine blue, Chicago sky blue, chlorophenol red, chrome azurol S, chromotrope 2B, chromotroye 2R, chromuxane cyanine B, chrysoidin, chrysophenine, cibacron brillant red 3BA, Congo red, copper (li) ploihalocyanine, cresol purple, cresol red, cresol, cresolphthalein, cresolphihalein complexone, crystal violet, curcumin, darrow red, diarninoacridine hemisulfate, diazo red RC, dibromofluorescein, dichlorofluorescein, dichloroindophenol, dicinnamaiactone, dietitytaminomethyt coumarin, diethyloxacarbocyanine iodide, diethylthiatricarbocyanine iodide, dihydroxy benzenesulfonic acid, dilithium phthalocyanine, dimethyl rnethylene blue, dimethylglyoxime, dimethylindoaniline, dinitro diphenylarnine, diphenylthiocarbazone, direct blue 71, direct green 6, direct red 23, direct red 75, direct red 81, direct violet 51, direct yellow 62, disodium phthalocyanine, disperse blue 14, disperse blue 3, disperse orange, disperse orange 11, disperse orange 25, disperse yellow 7, emodin, eosin B, eosin Y, eriochrnme black T, eriochrome blue black B, erioglaucine, erythrosin B, ethyl eosin, ethyl orange, ethyl red, ethyl violet, Evans blue, fast black, fast blue B salt, fast blue BB, fast blue RR, fast blue RR salt, fast corinth V salt, fast garnet GBC base, fast green FCF, fast red aluminum salt, fast red violet LB salt, fast violet B salt, fat brown RR fat green GDC salt, flavazin I, fluorescein, fluorexon, gallocyanine, guinea green B, hematoxylin, hydroxy naphtol blue, 1,4-hydroxy-naphthoquinone, indigo, indigo carmine, indoline blue, iron (ii) phthatocyaninH. Janus green B, lacmoid, leishman stain, leuco crystal violet, leucomalachile green, leuco4uir1izarin, light green SF yellowish, lissarnine green B, litmus, luxol fast blue, malachite green base, malachite green hydrochloride, malachite green oxalate, melanill yellow, rnelhyl eosin, methyl green, methyl orange, methyl red, rnethyl violet 2B, rnethyl violet B base, methyl yellow, methylene blue, methylene green, methylene violet 3RAX, methylesculetin, methylthymol blue, mordant blue 9, mordant brown 24, mordant brown 4, mordant orange, mordant orange 1, mordant orange 6, mordant red 19, mordant yelluw 10, morin hydrate, rnurexide, naphthochrome green, naphthol AS, naphtol blue black, naphtol green B, naphtol yellow, naphtholbenzein, naphtholbenzene, naphtholphihalein, neutral red, new coccine, new fuchsin, new rnethylene blue N, nigrosin,

Nile blue A, Nile blue chloride, nitrazine yellow, nitro red, nitro-phenanthroline, nitrophenol-2, nitrophenol-3, nitrophenol-4, nitrophenylazo-resorcinol, nuclear fast red, oil blue N, oil red EGN, oil red O, orange G, orange 11, palatine chrome black 6BN, palatine fast yellow BLN, pararosaniline acetate, pararosaniline base, pararosaniline chloride, patent blue VF, pentarnethoxytriphenylnoethanol, phenanthroline, phenazine, phenol red, phenolphthalein, phenolphthaleir diphosphate, phenothiazine, phenylazoaniline, phenylazodiphenylamine, phenylazoforrnic acid, phenylazophenol, phloxine B, phthalocynine, pinacyanol chloride, plasrnocorinth, ponceau S, primuline, procion red MX-5B, procion yellow H-E3G, prussian blue, purpurin, pyridlazo naphthol, pyridylazoresorcinol sodium salt, pyrocatechol violet, pyrogallol red, pyronin B, quinaldine red, quinizarin, quinoline yellow, reactive black 5, reactive blue 15, reactive blue 2, reactive blue 4, reactive orange 16, resazurin, resorcin crystal violet, rhodamine B, rhodamine B base, rhodamine GG, rhodamine S, rhodanine, rosalic acid, rose bengal, rose bengal iactone, safranine O, solvent blue 35, solvent blue 59, solvent green 3, styryl 7, sudan black B, sudan orange G, sudan red 7B, sulfobromophthalein sodium salt, sulforhodamille B, tartrazine, tetrabroìnophenol blue, tetrabromo phenolphthalein, tetrabromo phenolphthalein, tetraiodo phenolphthalein, tetraphenyl- butadiene, tetrazoliurn violet, thiazol yellow G, thioflavin S, thioflavin T, thionin, thymol blue, thymolphthalein, thymolphthalein rnonophosphate, thymolphthalein monophosphate, toluidine blue O, triphenylmethyl bromide, tropaelin O, trypan blue, turmeric, vanillin azine, variamine blue RT salt, variamine blue RT salt, victoria blue B, victoria blue B, victoria pure blue BO, wright stain, xilidine ponceau 2R"xylenol blue, and xylenol orange.

Some of these dyes are fluorescence dyes and there was a change in fluorescence. The reduced and oxidized forms of the above dyes can also be used.

Indicators: Any material, which undergoes a color change with the reaction product of ethylene oxide and ETO activator, can be used an ETO indicator. Most preferred classes of ETO indicators are dyes, pigments and their precursors. The use of a pH sensitive dye will depend upon the pH of the medium. Some dyes do not change color in EC001270 while they do change color in polyvinylalcohol under the identical conditions. The pH of the dry coating of EC001270 is about 5 while that of polyvinylalcohol is about 7.

Activators: Any c : hemical, which produces at least one indicator reactive species, e. g., a base such as sodium hydroxide, upon exposure to ETO, can be used as ETO activator. Alkali metal salts, such as sodium thiocyanate and quarternary ammonium salts, such as TEAB were effective activator. ETO activators, such as NaSCN, tetrabutylphosphonium trornide, tetrabutylphosphonium bromide and TEAB, are also referred to as activators hereill. Production of indicator reactive species, such as a base, can introduce a color change in a pH-sensitive dye. A variety of classes of organic and inorganic

compound were exploreci as activators for monitoring ETO. They include organic inorganic salts, such as bisulfites, bisulfates, carbonates, carbamates, carboxylates, cyanates, halides, nitrites, nitrates, phenolates, phosphates, sulfates, sutfides, sulfites, and thiocyanates.

Organic and inorganic salts, especially halides and thicyanates of (1) alkali metals and (2) organic ammonium, suitonium and phosphonium were more effective activators. Salts of acids with amines, such as tetramethylhexanediamine hydrobromide and tetramethylhexanediamine acetate were also effective ETO activators. Metal chelates and complexes, such as sodium acetylacetonates were also effective ETO activators.

The specific examples of compound explore as ETO activators with some selected dyes (e. g., brornophenol blue and bromocresol purple) inctude alurninurn acetylacetonate, aluminium ammonium sulfate, alurninum chloride, aluminurn sulfate, ammonium acetate, ammonium bisulfite, ammonium bromide, ammonium carbamate, ammonium nitrate, ammonium sulfarnate, ammonium sulfite, ammonium thiocyanate, ammonium thiosulfate, calcium ferrocyanide, copper thiocyanate, iron acetylacetonate, iron complexes such as potassium terrocyanide, iron sulfate, sodium acetylacetonate, sodium bisulfite, sodium cyante, sodium diethyldithio carbamate, sodium diethyldithiocarbamate, sodium dithionite, sodium hydrosulfide, sodium nitrite, sodium persulfate, sodium sulfite, sodium sulfite, sodium sulfite, sodium sulfite, sodium thiocyanate, sodium thiocyanate, sodium thiosulfate, sultosalicyclic acid, and tetrabutylphosphoniurn brornide. The activators also included a variety of primary, secon (iary and tertiary, aliphatic and aromatic amines neutralized with organic arid inorganic acids.

Effect of halides : Many organic and inorganic halides are highly sensitive indicator activators. These valides include, acetyl choline chloride, ammonium bromide, choline chloride, choline iodide, dodecyltrimethylarnmonium bromide, glycidil trimethyl ammonium chtoride. potassium bromide, potassium iodide, sodium iodide, tetrabutyl ammonium iodide, tetraethyl ammonium bromide, tetrahexyl ammonium bromide, tetrarnethyl amrnonium chloride and tetrabutyl pfnosphonium bromide. Generally iodide were more bromides and bromides were more effective than chlorides.

Acid base salts, e. g., those produced by neutralizing, prirnary, secondary and tertiary amines (e. g., hexyl amine, diethanolamine, tetramethylhexane diamine) with acids such as acetic acid, hydrochloric acid, hydrobromic acid and hydroiodic acid are also effective activators.

The time required for the color change can be controlled by using a proper mixture of the activators. In order to minimize effect of ethylene oxide lower concentration of halides is preferred for certain dyes.

Activators couid be polymeric, such as polyacrylic : acid co-polyallylammor1ium chloride and polybrene.

The time required for the color change can be varied by selecting a proper mixture of halides, thiocyanates, acetates, citrates etc.

Effect of concentration of activator: With certain dyes as low as 0.5% of TEAB and sodium iodide was effective in introducing a noticeable color change with ETO. The rate of the color change increases with increasing the concentration of the activator. One can use 0.5 to 50 w/w% concentration of an activator. Preferred concentration range is 2-20 w/w% of the total solid.

Polymers used as binders: A matrix or a medium in which the activators, indicators and additives can be dissolve or dispersed are referred herein as an ETO binder. ETO binders are also referred herein to as binders, polymers or polymeric binders. A wide variety of polymeric materials can be used as binders for the indicator as long as the activators and indicators can be dissolved or dispersed in them. Both aqueous and non-aqueous binders can be used. Though one can use water-soluble, water-dispersible and water-insoluble polymers as binders for the indicator, it is preferred to use water-soluble and water- dispersibie polymers as binders. The binders can be formulated in form ink formulations, such as flexo and gravure inks. Other inks such as those for letter press, offset and screen printing can also be used. Selection of the polymer depends upon the printing/coating equipment to be used.

Usually acrylic polymers, emulsion of acrylic polymers, occasionally natural polymers, such as starc : hes, lignins, and lignin derivatives are used as binders for inks.

Resins are water soluble or emulsifiable through neutralization with basic compounds, such as ammonia and amines. inks contain a variety of additives to eliminate foaming, dispersion of pigments, rheoiogicai modifiers, and slip agents.

Polymeric binders for inks include homopolymers, copolyrners and block-copolymers including those of ethylene acrylic acid, ethylene methacrylic acid, ethylene n-butyl acrylate, and ethylene methyl acl-ylate. Binders for inks could also be a mixture of homo and copolymers, e. g., those of methylmethacrylate, acrylic acid, styrene, methyl acrylate, other esters and crosslinking agents, such as polyvinylazaridine. Also included are polymers of acrylates, acrylic acid, acrylamide, vinyl acetate, vinyl alcohol, vinyl chloride, polyurethanes, cellulose nitrate, carboxymethyl cellulose or a mixture thereof. Desirably, the polymer is an acrylate polymer, cellulose nitrate or carboxyrnethylcellulose

Commercial sources for suitable polymers for ink formulations include Air products (Allentown, PA), Rohm and Haas (Philadelphia, PA), S. C. Johnsons and Sons (Racine, WI), Witco (Houston, PA) and ES ! (Valley Stream, NY). Though a large number of polymers are suitable an ink extender, EC001270 made by Environmental Inks and Coating Co., Lithicam, MD which is composed about 40% styrene-acrylic polymers, a few percent ammonium hydroxide, additives, such as wax and alcohol and the balance water, has been found very su itable.

The nature of the binder, e. g., pH and permeability to the gases, plays an important role. Color change of broinophenol blue and TEAB system with ETO is slower in EC001270 (an acrylate) than that in polyurethane (Witcobond 213, Witco Corporation, Houston, TX) and polyvinylalcohol. Permeability of the reactive gases can also be controlled by addition of a crosslinking agent and other additives, such as plasticizers which change the permeability of ETO.

Though aqueous ink or coating formulations are preferred, one can use solvent based coating formulations polymers used in such formulations are cellulose nitrate, carboxymethyl cellulose, polyolefins, polyvinylchloride, polyurethane, polysilicones and polyepoxy.

Binder prepared by UV curing: As an alternative to the aforesaid binders, one can use ink and coating formulation curable with UV light. UV curable ink and coating formulations include UV polymerizable/curable compound such as epoxy-acrylate, polyester acrylates, and resins, typically the acrylates of diphenylol propane di-glycidyl ethers, as their principal component. In order lower viscosity and to provide a bridge between large polymer molecules, acrylic molaolners are used, typically the acrylate esters of polyfunctional alcohols or glycols. The use of monomers as crosslinking agents is vital to the rapid formation of cured films, and has a major influence on the properties of both the ink or coating, and the cured product. Printing inks with epoxy-acrylate resins as their main cornponent are usually fast curing. In order to prepare the device, one can dissolve or disperse, the indicator, activator, and additives in the UV curable extender followed by coating on substrate and curing with UV light.

UV absorber: If an indicator formulation is sensitive to ultraviolet iight, UV absorbing materials can be added to minimize the effect. A large number of UV absorbers are available comrnercially, e. g., those used in sun-tan lotions, e. g., Tinuvins of Ciba-Geigy Corporation.

UV absorbers include compounds such as maleic acid, sodium salicylate, benzophenone, or benzophenone tetracarboxylate and a large number of polyaromatic compound. These UV absorbers cann't be used where the polymer for binding is prepared by UV curing.

Effect of topcoat: The sterilization of an article will also depend on diffusion of ETO through the binder. Hence, the time required for the color change of the device can be increased by applying a barrier coat on the device. A barrier coat can preferably be a polymeric material. The preferred barrier coat is a lacquer or an ink without pigment. The barrier coat can be a polymer listed herein. The generai classes of polymers suitable for a barrier coat include resins, such as epoxy, phenol-formaldehyde, amino-formaldehyde, polyamicies, vinyls, acrylics, polyurethanes, polyesters, water-soluble resins, alkyds, elastomers, and rosins. Preferred material for topcoat is a halopolymer through which ETO can diffuse slowly.

Two-layer device: The device can have more than one indicator layers each containing indicator, activator and binders. In order to get more than one color change at least the indicator should be different in different indicator layers and should undergo different color changes.

Both layers do not have to undergo color changes with ETO. Even if one layer undergoes a color change, a color change can be noticed, especially if the top layer becomes colorless.

Mixtures: Desired colors and color changes can be obtained by mixing proper dyes in appropriate amonts. Similarly, the time required the color change can be varied by using a proper mixture of the activators and additives in appropriate amounts. The desired colors and the time required for the color changes can be obtained by selecting a proper mixture of compatit) le bindels, additives arld activators.

Substrate: Though the device could be a self-supporting polymer film containing the activator and indicator, it is desirable to prepare the device on substrate. The device can also be made by coating the indicating formulation on a substrate. The substrate could be any solid surface; for exarnple, that made from paper, plastic, ceramic and metal. The substrate could be a container, e. g., bag or pouch, for items to be sterilized. The sterilization indicator can also be prepared in form of stickers.

Although any solid substrate having a smooth surface can be used, a preferred substrate is a flexible and transparent plastic film, and natural (cellulose) and synthetic (e. g., spun bonded polyolefins, e. g., TyvekR) papers. Plastic filrns, such as polyethylene, polypropylene, polyvinylchloride, polymethylmethacrylate, polyurethanes, nylons, polyesters, polycarbonates, polyvinylacetate, cellophane and esters of cellulose can be used as the transparent substrate. Metal foils, such as aluminum can be used. The most preferred substrats are the 5-300 microns thick films of polyethylene terephthalate, cellulose paper and I-yv (,-k

pH ranges: The pH of the system depends upon the indicator, activator, binder and additive. If the binder is acidic, e. g., polyacrylic acid or an acid as an additive, e. g., citric acid, the pH of the system will be low, e. g., pH of 2 to 3. However, on the other hand, if the basic system is used, e. g., polyethyleneimine as a binder or a base such as sodium hydroxide or an amine such as diethanolamine is added as an additive, the pH of system could be high, e. g., pH of 10 to 12. The indicator, e. g., a pH sensitive dye, to be selected would depend upon on the pH of the coating. Production of a base upon exposure to ETO would shift the pH of the system towards the basic side. The indicator should under go at least one color change above the pH of the binder system containing the indicator, activator and additive.

The pH range of the system could be 3 to 11. The preferred pH range of the system is 5-9.

The most preferred pH range of the system is 6-8. The change in pH of the system upon exposure to ETO could be as much as 5, e. g., from 4-9. The preferred change in pH is about 2, e. g., form 6 to 8 or 5 to 7.

Temperature: The indicator can undergo color change from a very low temperature (e. g.,-30"C) to a very high temperature of a hundred degrees centigrade. The preferred temperature is below about 70"C, preferred temperature is 20-60°C.

Time: The time required for the color change can be varied by varying one or more of parameters such as thickness of the binder and the indicator layer, concentration of the activator, concentration of the indicator, concentration of other additives, nature of the binder, nature of the barrier, thickness of the barrier, nature of the activator, nature of the indicator, nature of the additives and concentration of ETO. For example, the time required for the color change can be increased by (1) decreasing the concentration of activator (2) selecting a slow activator, (3) additives which neutralize the reaction products, such as an acid or phenols and (4) selecting an indicator having higher pH range for the color change. Similarly, the time required for the color change can be decreased by (1) increasing the concentration of activator (2) selecting a laster aclivator, (3) additives, such as an amine, and (4) selecting an indicator having lower pi-l rallye for the color change.

For a given sterilization cycle, the time required for the color change can be varied by varying one or more of the following parameters (w/w = weight to weight percent) : 1. Thickness of the polyrner indicator layer.

The thickness of the indicator and barrier layers may vary from a micron to a few hundred microns. The preferred thickness is 1-50 microns and the most preferred range is 2- 20 microns.

2. Concentration of the activator.

The concentration of activator may vary from 0.1 to 50 w/w%. The preferred concentration is 1 to 20 w/w% and the most preferred concentration is 2-10 w/w/%.

3. Concentration of the indicator.

The concentration of the indicator may vary from 0.1 to 20w/w%. The preferred concentration is 1 to 10w/w% and the most preferred concentration is 2-5 w/w%.

4. Concentration of other additives.

The concentration of additives may vary from 0.1 to 20w/w%. The preferred concentration is 0.5 to 10w/w% and the most preferred concentration is 1-5w/w/%.

5. Nature of the polymer.

6. Nature of the barrier.

This is the same group as the polymer, but may be different from it 7. Thickness of the barrier.

The barrier may be between 2 and 200 microns thick, preferrably 2-20 microns.

8. Nature of the activator.

9. Nature of the indicator.

10. Nature of the additives.

11. Concentration of ETC: The time required for the color change will depend upon concentration of ETO gas.

ETO gas can be diluted with gases such as air, nitrogen, angon, carbon dioxide, fluorocarbons and water vapor (humidity). The time required for the color change will be shoiler with higher concentration of ETO and vice versa. Higher humidity will decrease the time required forthe color claange.

The preferred time range for sterilization is from 10 minutes to 10 hours, The most preferred time is about 30 minutes to five hours.

Monitoring ETO: In order to protect workers who use ETO while on the job, the Occupational Safety an Health Administration (OSHA) has established a lirnit of 1 ppm in workplace air for an 8-hour workday and a liait of 5 ppm for a 15-minute period. A filter paper coated with a mixture ot bromothymol blue and potassium iodide changes from faint yellow to green-blue within minutes at about 100 parts per million.

The device can be used for monitoring low concentration of ETO. Though we demonstrated the concept with ETO, the device can also be used with other epoxides.

Advantages: The device offers some of the major advantages.

*. The formulations are inexpensive.

*. The ingredients are considered nontoxic.

*. It is easy to make the ink formulations, just by mixing proper ingredients in an ink extender.

*. The device is selective to ETO. It is unaffected by steam, heat and radiation.

*. It is unaffected by sealing hot bar.

*. in huas required pot life.

*. There is no bleeding/diffusion of dyes.

*. The ingredients (indicators/dyes and activators/additives) are water soluble. No grinding of ingredients required.

*. Ink is printable with gravure and flexo presses on polyester, paper and Tyvek (g).

*. The print rolls are easy to clean.

*. The time required for the color change can be varied by simple means.

*. It provides desired color changes (frorn a starting light color, such as white/colorless, yellow, orange, pink, or red to a final dark color, such as blue, green, black, purple or violet).

*. It provides an color for monitoring a partial cycle

EXAMPLES Example 1. General procedure for preparation of the sample devices.

In a 10m1 test tube were added about 3 mi of EC001270 (an acrylate ink extender supplied by Environmental inks and Coating, Co, Lithicum, MD), about 0.5 mi of an activator solution (e. g., 50w/w% TEAB in water) and about 0.5 ml of an indicator solution (e. g., 4 w/w% solutions of a dye, e. g., bromocresol purple in ethanol). The contents were mixed and coated with either #5 or #10 wire wound rod on a 100 micron polyester film and paper. The coatings were dried in an oven at about 50°C for about a few minutes.

Example 2. Exposure to ETO.

Samples of example 1 were placed in cell (e. g., 30 x 30 x 1 cm') and flushed with 100% ETO gas for a few minutes. The color changes of the samples were noted.

Some selected samples were also exposed to 10% ETO (90% a chlorofluoro gas) at 140°F, 100% humidity.

Example 3: Effect of activator on dyes.

Using the general procedure described in example 1, coatings were prepared from EC001270 as a binder, TEAB as an activator, and most of the dyes listed in Table 1 as indicators. The coatings were exposed to ETO for a few hours. Some representative color changes are listed Table 2.

Table 2. Representative color changes of sorne dyes with EC001270 and TEAB Dye Original color Color after ETO exposure Victoria Blue B Blue Brown Leuco Crystal Violet Blue Colorless Basic blue 66 Blue Red Naphtholbenzene alpha Brown Green Naphtholphthalein-alpha Colorless Green Lissamine Green B Gray Clear Brilliant Green Green Colorless Guinea Green B Green Colorless Chromoxane Cyanine R Lavender Yellow Quinizarin Peach Lavender Rosalic Acid Peach Pink Alizarin Red Peach Purple Acid Fuschin Pink Colorless Acid Red 1 Pink Red Methyl Red Pink Yellow Palatine chrome black 6BN Purple Blue Bromocesol Purple Yellow Blue Bromophenol Blue Yellow Blue Brornothy-nol Blue Yellow Blue Thiazol Yellow G Yellow Brown Cresol Red Yellow Purple Alizarine Yellow R Yellow Red Phenol Red Yellow Red The color change usually occurs selectively with ETO. There is little effect of steam and radiation.

Similar color changes were obtained with many other activators, such as sodium thiocyanate, and tetrabutylphosphonium bromide, and binders e. g., polyurethane (Witcobond 213, Witco Corporation, Houston, TX) and cellulose nitrate.

Example 4. Effect of other halides.

Using the procedure described in example 1, coatings were prepared with some organic and inorganic hatides as activators and bromophenol blue and bromocresol purple as indicators. The coatings were exposed to ETO. All coatings changed from yellow to blue. iodide and bromides were more effective than other halides in introducing the color change.

The halide tried included, acetyl choline chloride, ammonium bromide, barium bromide, calcium bromide, choline chloride, choline iodide, dimethyldioctadecylammonium bromide, diphenyliodiniurn bromide, dodecyltrimethylammonium brornide, glycidil trimethyl ammonium chloride, iron (ici) bromide, phenacylpyridinium bromide, potassium bromide, potassium bromide, potassium iodide, sodium iodide, tetrabutyl ammonium iodide, tetrabutyl phosphonium bromide, tetrabutylammonium bromide, tetraethyl ammoniurn bromide, tetrahexyl ammonium bromide, tetramethyl ammonium chloride, tetramethylhexaenediamine hydrobrornide, tetrafnethythexanediamine hydroiodide, and zinc bromide.

Example 5. Device without binder.

Fifty (w/w) percent solutions of tetrabutylphosphoniurn bromide (PB), sodium thiocyanate (NaSCN) ancJ teiraethylammonium bromide (TEAB) were prepared in water.

Pieces of filter paper were dipped in the solutions. Drops of a universal pH indicator (Fisher Scientitic) were placed on the coated paper filter papers. The filter papers were dried in an oven at 50"C for 1 hour. The filter papers were then exposed to ETO gas. The color change occurred within seconds when exposed to ETO. The color change was fastest with sodium thiocyanate and slowest with tetraethyi ammonium bromide. The color changes are reported below: Table 3. The color changes of a universal pH indicator with different activators.

PB NaSCN TEAB Color Original Red Red Yellow Exposed Green Violet Green

Example 6. Detection of residual absorbed ETO.

A piece of 100-micron poiyester film having a coating of EC001270 was exposed to ETO for ten minutes. The coated film was removed and hang in air for ten minutes. A coating of EC001270, bromophenol biue and TEAB on polyester was placed on ETO exposed film.

The yellow coating turned green within 5 minutes.

Example 7. Mixture of two indicators.

Using the procedure described in example 1, a coating was prepared using ethyl red, bromophenol blue and their 1: 1 mixture as indicators and TEAB as an activator. The ethyl red coating changed from red-to-yellow, that of bromophenol blue changed from yellow-to-green- to-blue and that of their mixture changed from orange--> yellow--> yellow green--> green.

Using the procedure described in example 1, a coating was prepared using bromophenol blue as an indicator and 1: 1 mixture of TEAB and NaSCN as activators. The coating was yellow. The coating changed from yellow-to-green-to-blue upon exposure to ETO.

Example 9. Pilot coating.

In 13kg EC001270 extender the following were added while stirring : 200g of 28% ammonium hydroxide, 3kg of water, 2kg of tetraethylammonium brornide, 2kg of sodium thiocyanate, 100g of bl-onlocresol purple, and 135g of bromothymol blue. The formulation was coated on paper at Rexam Medical Packaging, Mundelein, IL.

The samples were exposed to 100% ETO. The indicator changed from yellow-to- green-to-blue. The indicator has very little effect of (1) dry heat at 60°C five days, (2) 100% relative humidity at 60"C for five days, (3) normal steam sterilization treatment (121"C, 30 minutes). The final blue color was essentailly unaffected after 5 days at 50°C at 100% RH.

Clearly, it should now be quite evident to those skilled in the art, that while this invention has been shown and described in detail in the context of a preferred embodiment, and with various modifications thereto, a wide variety of other modifications can be made without departing from scope of the inventive teachings.