Title:
ENCRYPTION OF TRAITS USING SPLIT GENE SEQUENCES
Document Type and Number:
WIPO Patent Application WO2000052146
Kind Code:
A3
Abstract:
Methods of unencrypting trait encrypted gene sequences to provide unencrypted RNAs or polypeptides. The invention also relates to methods of encrypting traits including splitting genes between two parental organisms or between a host organism and a vector. The gene sequences are unencrypted when the two parental organisms are mated or when the vector infects the host organism by trans-splicing either the split RNAs or split polypeptides upon expression of the split gene sequences. The invention also includes methods of providing multiple levels of trait encryption and reliable methods of producing hybrid organisms. Additional methods include those related to unencrypting engineered genetic elements to provide polypeptide functions and those directed at recombining non-overlapping gene sequences. The invention also includes integrated systems and various compositions related to the disclosed methods.
Inventors:
PATTEN PHILLIP A (US)
LASSNER MICHAEL (US)
YAMAMOTO TAKASHI (US)
CARR BRIAN (US)
NESS JON E (US)
BERMUDEZ ERICKA R (US)
LASSNER MICHAEL (US)
YAMAMOTO TAKASHI (US)
CARR BRIAN (US)
NESS JON E (US)
BERMUDEZ ERICKA R (US)
Application Number:
PCT/US2000/005448
Publication Date:
July 19, 2001
Filing Date:
March 03, 2000
Export Citation:
Assignee:
MAXYGEN INC (US)
PATTEN PHILLIP A (US)
LASSNER MICHAEL (US)
YAMAMOTO TAKASHI (US)
CARR BRIAN (US)
NESS JON E (US)
BERMUDEZ ERICKA R (US)
PATTEN PHILLIP A (US)
LASSNER MICHAEL (US)
YAMAMOTO TAKASHI (US)
CARR BRIAN (US)
NESS JON E (US)
BERMUDEZ ERICKA R (US)
International Classes:
C12N15/10; C12N15/82; C12P21/04; (IPC1-7): C12N9/00; A01H1/00; A01H5/00; C12N9/14; C12N15/10; C12N15/62; C12N15/82; G06F17/50
Domestic Patent References:
| WO1995007351A1 | 1995-03-16 | |||
| WO1992013089A1 | 1992-08-06 | |||
| WO2000017342A2 | 2000-03-30 |
Other References:
WU H ET AL: "Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA., vol. 95, August 1998 (1998-08-01), WASHINGTON., US, pages 9226 - 9231, XP002137477, ISSN: 0027-8424
SOUTHWORTH M W ET AL: "Control of protein splicing by intein fragment reassembly", EMBO JOURNAL, OXFORD, GB, vol. 17, no. 4, 1998, pages 918 - 926, XP002121550, ISSN: 0261-4189
SHINGLEDECKER K ET AL: "Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments", GENE, BARKING, GB, vol. 207, no. 2, 30 January 1998 (1998-01-30), pages 187 - 195, XP004108918, ISSN: 0378-1119
EVANS T C ET AL.: "Semisynthesis of cytotoxic proteins using a modified splicing element", PROTEIN SCIENCE, vol. 7, 1998, US, pages 2256 - 2264, XP002925638
SOUTHWORTH M W ET AL: "Control of protein splicing by intein fragment reassembly", EMBO JOURNAL, OXFORD, GB, vol. 17, no. 4, 1998, pages 918 - 926, XP002121550, ISSN: 0261-4189
SHINGLEDECKER K ET AL: "Molecular dissection of the Mycobacterium tuberculosis RecA intein: design of a minimal intein and of a trans-splicing system involving two intein fragments", GENE, BARKING, GB, vol. 207, no. 2, 30 January 1998 (1998-01-30), pages 187 - 195, XP004108918, ISSN: 0378-1119
EVANS T C ET AL.: "Semisynthesis of cytotoxic proteins using a modified splicing element", PROTEIN SCIENCE, vol. 7, 1998, US, pages 2256 - 2264, XP002925638
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