BASU SHIB SANKAR (US)
SMITH JOHN PAUL (US)
VALENCIA CESAR U (US)
SCREEN STEVEN E (US)
VASSALLO CARLOS (US)
WO2014028520A1 | 2014-02-20 |
US20220053770A1 | 2022-02-24 | |||
CN111286480A | 2020-06-16 | |||
CN111893075A | 2020-11-06 | |||
US20210321621A1 | 2021-10-21 | |||
US6681186B1 | 2004-01-20 |
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compr ses an endop yte etero ogous y d sposed to a p ant e ement, w ere n t e endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.5-51, or combinations thereof. 2. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is 100% identical to SEQ ID NO.5, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs. 6-51. 3. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to each of SEQ ID NOs.5-51. 4. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is at least 100% identical to each of SEQ ID NOs.5-51. 5. The synthetic composition of claim 1, wherein the one or more endophytes comprise one or more a polynucleotide sequences that are 100% identical to a subregion within one or more of SEQ ID NOs.5-51, wherein the subregion is a 100, 200, 300, 400, 500, 600, 700, or 800 nucleotides in length. 6. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is 100% identical to SEQ ID NO.5, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs. 6, 7, 8, 9, 10, 11, 12, 13, 14, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.15, 16, 17, 18, 19, 20, 21, 22, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.23, 24, 25, 26, 27, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.42, 43, 44, 45, 46, 47, 48, 49, 50, 51, and at least one polynucleotide sequence that is at least 97% identical SEQ ID NO.40, and at least one polynucleotide sequence that is at least 97% identical SEQ ID NO.41, 7. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs. 6, 7, 8, 9, 10, 11, 12, 13, 14, or combinations thereof. 8. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs. 15, 16, 17, 18, 19, 20, 21, 22, or combinations thereof. 9. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs. 23, 24, 25, 26, 27, or combinations thereof. 10. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs. 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or combinations thereof. 11. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs. 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, or combinations thereof. 12. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% to SEQ ID NOs.40. 13. The synthetic composition of claim 1, wherein the endophyte comprises at least one artificially introduced genetic modification to SEQ ID NOs.40. 14. The synthetic composition of claim 1, wherein the modified endophyte is capable of producing a peptide having an amino acid sequence selected from SEQ ID Nos.100- 134. 15. The synthetic composition of claim 1, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% to SEQ ID NOs.41. 16. The synthetic composition of claim 1, wherein the endophyte comprises one or more genes encoding a protein whose amino acid sequence comprises an amino acid sequence selected from SEQ ID NOs.52-134. 17. The synthetic composition of claim 1, wherein the endophyte comprises one or more genes encoding a protein whose amino acid sequence is selected from SEQ ID NOs. 97-134. 18. The synthetic composition of claim 1, wherein the endophyte is present in an amount capable of improving a trait of agronomic importance in a plant. 19. The synthetic composition of claim 18, wherein the trait of agronomic importance is selected from the group consisting of yield, root fresh weight, shoot fresh weight, biotic stress tolerance, drought tolerance, and combinations thereof. 20. The synthetic composition of claim 18, wherein the trait of agronomic importance is biotic stress tolerance. 21. The synthetic composition of claim 20, wherein biotic stress tolerance is a growth environment comprising a fungal pathogen. 22. The synthetic composition of claim 20, wherein the biotic stress tolerance is shown by increased emergence, increased biomass, increased NDVI, decreased pathogen incidence, decreased necrosis, decreased chlorosis, decreased area of necrotic tissue, decreased area of chlorotic tissue, or increased yield. 23. The synthetic composition of claim 21, wherein the pathogen is one or more pathogens of the genus Pythium, Rhizoctonia, Fusarium, Cercospora, Colletotrichum, Dreschslera, Corynespora, Diaporthe, and Macrophomina. 24. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+2 CFU/g. 25. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+3 CFU/g. 26. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+4 CFU/g. 27. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+5 CFU/g. 28. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+6 CFU/g. 29. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+7 CFU/g. 30. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+8 CFU/g. 31. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+9 CFU/g. 32. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration between 1.0E+5 CFU/g and 1.0E+10 CFU/g. 33. The synthetic composition of claim 1, wherein the endophyte is present in the synthetic composition in a concentration between 1.0E+6 CFU/g and 1.0E+9 CFU/g. 34. The synthetic composition of claim 1, further comprising a plant element. 35. The synthetic composition of claim 34, wherein the plant element is a monocot. 36. The synthetic composition of claim 35, wherein the monocot is a cereal. 37. The synthetic composition of claim 36, wherein the cereal is selected from the group consisting of wheat, rice, barley, buckwheat, rye, millet, oats, corn, sorghum, triticale and spelt. 38. The synthetic composition of claim 37, wherein the cereal is corn. 39. The synthetic composition of claim 34, wherein the plant element is a dicot. 40. The synthetic composition of claim 39, wherein the dicot is selected from the group consisting of cotton, tomato, lettuce, peppers, cucumber, endive, melon, potato, and squash. 41. The synthetic composition of claim 39, wherein the dicot is a legume. 42. The synthetic composition of claim 41, wherein the legume is soy, peas, or beans. 43. The synthetic composition of claim 1, further comprising one or more endophytes. 44. The synthetic composition of claim 1, wherein the plant element is a whole plant, seedling, meristematic tissue, ground tissue, vascular tissue, dermal tissue, seed, leaf, root, shoot, stem, flower, fruit, stolon, bulb, tuber, corm, keikis, or bud. 45. The synthetic composition of claim 34, wherein the plant element is a seed. 46. The synthetic composition of claim 1, wherein the endophyte is dead. 47. The synthetic composition of claim 1, additionally comprising one or more of a surfactant, a buffer, a tackifier, a microbial stabilizer, a fungicide, an anticomplex agent, an herbicide, a nematicide, a fertilizer, an insecticide, a plant growth regulator, a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt, and a polymer. 48. The synthetic composition of claim 47, wherein the fertilizer is granular or liquid in form and comprises one or more of nitrogen, phosphorous, potassium, sulfur, magnesium, silica, iron, zinc, manganese, copper, boron, and fulvic acid. 49. A method of treating an agricultural plant, comprising heterologously disposing a plant element or planting media with a synthetic composition comprising an endophyte, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.5-51, or combinations thereof. 50. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is 100% identical to SEQ ID NO.5, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.6-51. 51. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to each of SEQ ID NOs.5-51. 52. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is at least 100% identical to each of SEQ ID NOs.5-51. 53. The method of claim 49, wherein the one or more endophytes comprise one or more a polynucleotide sequences that are 100% identical to a subregion within one or more of SEQ ID NOs.5-51, wherein the subregion is a 100, 200, 300, 400, 500, 600, 700, or 800 nucleotides in length. 54. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is 100% identical to SEQ ID NO.5, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.6, 7, 8, 9, 10, 11, 12, 13, 14, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.15, 16, 17, 18, 19, 20, 21, 22, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs. 23, 24, 25, 26, 27, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, and at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.42, 43, 44, 45, 46, 47, 48, 49, 50, 51, and at least one polynucleotide sequence that is at least 97% identical SEQ ID NO.40, and at least one polynucleotide sequence that is at least 97% identical SEQ ID NO.41, 55. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.6, 7, 8, 9, 10, 11, 12, 13, 14, or combinations thereof. 56. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.15, 16, 17, 18, 19, 20, 21, 22, or combinations thereof. 57. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.23, 24, 25, 26, 27, or combinations thereof. 58. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, or combinations thereof. 59. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% identical to one or more of SEQ ID NOs.42, 43, 44, 45, 46, 47, 48, 49, 50, 51, or combinations thereof. 60. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% to SEQ ID NOs.40. 61. The method of claim 49, wherein the endophyte comprises at least one artificially introduced genetic modification to SEQ ID NOs. 40. 62. The method of claim 49, wherein the modified endophyte is capable of producing a peptide having an amino acid sequence selected from SEQ ID Nos.100-134. 63. The method of claim 49, wherein the endophyte comprises at least one polynucleotide sequence that is at least 97% to SEQ ID NOs.41. 64. The method of claim 49, wherein the endophyte comprises one or more genes encoding a protein whose amino acid sequence comprises an amino acid sequence selected from SEQ ID NOs.52-134. 65. The method of claim 49, wherein the endophyte comprises one or more genes encoding a protein whose amino acid sequence is selected from SEQ ID NOs.97-134. 66. The method of claim 49, wherein the endophyte is present in an amount capable of improving a trait of agronomic importance in a plant. 67. The method of claim 66, wherein the trait of agronomic importance is selected from the group consisting of yield, root fresh weight, shoot fresh weight, biotic stress tolerance, drought tolerance, and combinations thereof. 68. The method of claim 67, wherein the trait of agronomic importance is biotic stress tolerance. 69. The method of claim 68, wherein biotic stress tolerance is a growth environment comprising a fungal pathogen. 70. The method of claim 68, wherein the biotic stress tolerance is shown by increased emergence, increased biomass, increased NDVI, decreased pathogen incidence, decreased necrosis, decreased chlorosis, decreased area of necrotic tissue, decreased area of chlorotic tissue, or increased yield. 71. The method of claim 69, wherein the pathogen is one or more pathogens of the genus Pythium, Rhizoctonia, Fusarium, Cercospora, Colletotrichum, Corynespora, Dreschslera, Diaporthe, and Macrophomina. 72. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+2 CFU/g. 73. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+3 CFU/g. 74. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+4 CFU/g. 75. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+5 CFU/g. 76. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+6 CFU/g. 77. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+7 CFU/g. 78. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+8 CFU/g. 79. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration of at least 1.0E+9 CFU/g. 80. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration between 1.0E+5 CFU/g and 1.0E+10 CFU/g. 81. The method of claim 49, wherein the endophyte is present in the synthetic composition in a concentration between 1.0E+6 CFU/g and 1.0E+9 CFU/g. 82. The method of claim 49, further comprising a plant element. 83. The method of claim 82, wherein the plant element is a monocot. 84. The method of claim 83, wherein the monocot is a cereal. 85. The method of claim 84, wherein the cereal is selected from the group consisting of wheat, rice, barley, buckwheat, rye, millet, oats, corn, sorghum, triticale and spelt. 86. The method of claim 85, wherein the cereal is corn. 87. The method of claim 82, wherein the plant element is a dicot. 88. The method of claim 87, wherein the dicot is selected from the group consisting of cotton, tomato, lettuce, peppers, cucumber, endive, melon, potato, and squash. 89. The method of claim 87, wherein the dicot is a legume. 90. The method of claim 89, wherein the legume is soy, peas, or beans. 91. The method of claim 49, further comprising one or more endophytes. 92. The method of claim 49, wherein the plant element is a whole plant, seedling, meristematic tissue, ground tissue, vascular tissue, dermal tissue, seed, leaf, root, shoot, stem, flower, fruit, stolon, bulb, tuber, corm, keikis, or bud. 93. The method of claim 82, wherein the plant element is a seed. 94. The method of claim 49, wherein the endophyte is dead. 95. The method of claim 49, wherein the synthetic composition additionally comprising one or more of a surfactant, a buffer, a tackifier, a microbial stabilizer, a fungicide, an anticomplex agent, an herbicide, a nematicide, a fertilizer, an insecticide, a plant growth regulator, a rodenticide, a desiccant, a nutrient, an excipient, a wetting agent, a salt, and a polymer. 96. The method of claim 95, wherein the fertilizer is granular or liquid in form and comprises one or more of nitrogen, phosphorous, potassium, sulfur, magnesium, silica, iron, zinc, manganese, copper, boron, and fulvic acid. 97. The method of claim 49, wherein the synthetic composition is heterologously disposed to a seed at a rate of 65-130 mL/100 kg seed. 98. The method of claim 49, wherein: the one or more endophytes are heterologously disposed to a plant element prior to placing the treated plant element in or on a growth medium, the one or more endophytes are heterologously disposed to a plant element after placing the plant elements in or on a growth medium, the one or more endophytes are heterologously disposed to a plant element concurrently with placing the plant elements in or on a growth medium, the one or more endophytes are heterologously disposed to a plant element at least two times, the one or more endophytes are heterologously disposed to a plant element via a seed treatment or soil pre-treatment and one or more foliar applications, the one or more endophytes are heterologously disposed to a plant element via a seed treatment or soil pre-treatment and one or more floral applications, the one or more endophytes are heterologously disposed to a plant element via one or more seed treatments or soil pre-treatments, one or more foliar applications, and one or more floral applications, the one or more endophytes are heterologously disposed to a plant element via seed treatment, root wash, seedling soak, foliar application, floral application, soil inoculum, in-furrow application, sidedress application, soil pre-treatment, wound inoculation, drip tape irrigation, vector-mediation inoculation, injection, osmopriming, hydroponics, aquaponics, or aeroponics, the one or more endophytes are heterologously disposed to a plant element of a different plant variety from the variety of the plant element from which the one or more endophytes were obtained, the one or more endophytes are heterologously disposed to a plant element of the same plant variety as the variety of the plant element from which the one or more endophytes were obtained, the one or more endophytes are heterologously disposed to a plant element of a different plant species from the species of the plant element from which the one or more endophytes were obtained, the one or more endophytes are heterologously disposed to a plant element of the same plant species as the species of the plant element from which the one or more endophytes were obtained, or the one or more endophytes are heterologously disposed as described in one or more of the above. 99. The synthetic composition of claim 1, wherein the endophyte is produced in a low nutrient growth media. 100. The method of claim 49, wherein the endophyte is produced in a low nutrient growth media. 101. The synthetic composition of claim 47, wherein the fungicide comprises one or more of fludioxonil, sedexane, and mefenoxam. 102. The method of claim 95, wherein the fungicide comprises one or more of fludioxonil, sedexane, and mefenoxam. |
gen y m xe un e s urry was even y sperse . Method of treating seeds with water dispersed formulations [0114] Water dispersed endophyte formulations comprise endophyte biomass in liquid fermentation broth that may be diluted in a buffered carrier such as phosphate buffered saline as well as a preservative and/or a pH adjusting agent. The volume of seeds was used to determine the volume of endophyte in water dispersion formulation needed for the target dose per seed. The calculated volume of endophyte formulation was added to the seeds in a clean mixing vessel. The seeds and endophyte formulation were mixed for at least 30 seconds to ensure the endophyte formulation was well dispersed on the seeds. Method of treating seeds with oil dispersed formulations [0115] Oil dispersion formulations comprise endophyte biomass, a vegetable oil-based carrier, a dispersant, and/or a rheology modifier. The volume of seeds is used to determine the volume of endophyte in oil dispersion formulation needed for the target dose per seed. The oil dispersed endophyte formulation is thoroughly agitated to resuspend the endophyte throughout the formulation. The calculated volume of endophyte formulation is added to the seeds in a clean mixing vessel. The seeds and endophyte formulation are mixed to ensure the endophyte formulation was well dispersed on the seeds. Method of treating seeds with flowable powder formulations [0116] Flowable powder endophyte formulations comprise talc, mineral oil base, desiccant (optionally), and spray dried or solid state fermentation produced endophyte. The volume of seeds was used to determine the volume of endophyte in a flowable powder formulation needed for the target dose per seed. The seeds to be treated were added to a clean mixing vessel. The calculated volume of endophyte formulation for the desired dose was added to the seeds in a clean mixing vessel. The seeds and endophyte formulation were mixed for at least 30 seconds to ensure the endophyte formulation was well dispersed on the seeds. Example 4. Additional methods for creating synthetic compositions. Osmopriming and Hydropriming [0117] One or more endophytes are inoculated onto seeds during the osmopriming (soaking in polyethylene glycol solution to create a range of osmotic potentials) and/or hydropriming (soaking in de-chlorinated water) process. Osmoprimed seeds are soaked in a polyethylene glycol solution containing one or more endophytes for one to eight days and then air dried for one to two days. Hydroprimed seeds are soaked in water for one to eight days containing one or more endophytes and maintained under constant aeration to maintain a suitable dissolved oxygen content of the suspension until removal and air drying for one to two days. Talc and or flowability polymer are added during the drying process. Foliar application [0118] One or more endophytes are inoculated onto aboveground plant tissue (leaves and stems) as a liquid suspension in dechlorinated water containing adjuvants, sticker-spreaders and UV protectants. The suspension is sprayed onto crops with a boom or other appropriate sprayer. Soil inoculation [0119] One or more endophytes are inoculated onto soils in the form of a liquid suspension, either; pre-planting as a soil drench, during planting as an in-furrow application, or during crop growth as a side-dress. One or more endophytes are mixed directly into a fertigation system via drip tape, center pivot or other appropriate irrigation system. Hydroponic and Aeroponic inoculation [0120] One or more endophytes are inoculated into a hydroponic or aeroponic system either as a powder or liquid suspension applied directly to the rockwool substrate or applied to the circulating or sprayed nutrient solution. Vector-mediated inoculation [0121] One or more endophytes are introduced in powder form in a mixture containing talc or other bulking agent to the entrance of a beehive (in the case of bee-mediation) or near the nest of another pollinator (in the case of other insects or birds). The pollinators pick up the powder when exiting the hive and deposit the inoculum directly onto the crop’s flowers during the pollination process. Root Wash [0122] The method includes contacting the exterior surface of a plant’s roots with a liquid inoculant formulation containing one or more endophytes. The plant’s roots are briefly passed through standing liquid microbial formulation or liquid formulation is liberally sprayed over the roots, resulting in both physical removal of soil and microbial debris from the plant roots, as well as inoculation with microbes in the formulation. Seedling Soak [0123] The method includes contacting the exterior surfaces of a seedling with a liquid inoculant formulation containing one or more endophytes. The entire seedling is immersed in standing liquid microbial formulation for at least 30 seconds, resulting in both physical removal of soil and microbial debris from the plant roots, as well as inoculation of all plant surfaces with microbes in the formulation. Alternatively, the seedling can be germinated from seed in or transplanted into media soaked with the microbe(s) of interest and then allowed to grow in the media, resulting in soaking of the plantlet in microbial formulation for much greater time, for example: hours, days, or weeks. Endophytic microbes likely need time to colonize and enter the plant, as they explore the plant surface for cracks or wounds to enter, so the longer the soak, the more likely the microbes will successfully be installed in the plant. Wound Inoculation [0124] The method includes contacting the wounded surface of a plant with a liquid or solid inoculant formulation containing one or more endophytes. Plant surfaces are designed to block entry of microbes into the endosphere, since pathogens attempt to infect plants in this way. One way to introduce beneficial endophytic microbes into plant endospheres is to provide a passage to the plant interior by wounding. This wound can take a number of forms, including pruned roots, pruned branches, puncture wounds in the stem breaching the bark and cortex, puncture wounds in the tap root, puncture wounds in leaves, puncture wounds in the seed allowing entry past the seed coat. Wounds can be made using tools for physical penetration of plant tissue such as needles. Microwounds may also be introduced by sonication. The microbial inoculant, as liquid, as powder, inside gelatin capsules, in a pressurized capsule injection system, or in a pressurized reservoir and tubing injection system, can then be contacted into the wound, allowing entry and colonization by microbes into the endosphere. Alternatively, the entire wounded plant can be soaked or washed in the microbial inoculant for at least 30 seconds, giving more microbes a chance to enter the wound, as well as inoculating other plant surfaces with microbes in the formulation – for example pruning seedling roots and soaking them in inoculant before transplanting is a very effective way to introduce endophytes into the plant. Injection [0125] The method includes injecting microbes into a plant to successfully install them in the endosphere. Plant surfaces are designed to block entry of microbes into the endosphere, since pathogens attempt to infect plants in this way. To introduce beneficial endophytic microbes to endospheres, we need a way to access the interior of the plant which we can do by puncturing the plant surface with a needle and injecting microbes into the inside of the plant. Different parts of the plant can be inoculated this way including the main stem or trunk, branches, tap roots, seminal roots, buttress roots, and even leaves. The injection can be made with a manual, mechanical, or biological injection system, and through the puncture wound can then be contacted the microbial inoculant as liquid, as powder, inside gelatin capsules, in a pressurized capsule injection system, or in a pressurized reservoir and tubing injection system, allowing entry and colonization by microbes into the endosphere. Example 5. Viability over time of endophytes in synthetic fertilizer compositions. [0126] This example describes an exemplary method by which compatibility of synthetic compositions comprising endophytes and fertilizers may be evaluated. [0127] Application rates. Fertilizer compositions may be granular or liquid in form and comprise nitrogen, phosphorous, sulfur, zinc, micronutrients, urease inhibitors, monoammonium phosphate (MAP), and/or triple superphosphate (TSP). Flowable powder (FP) endophyte treatments, prepared as described above, have a target application rate of 3.6 grams per acre. Water dispersal (WD) endophyte treatments, prepared as described above, have a target application rate of 13 grams per acre. Synthetic compositions are prepared using concentrations of endophyte and fertilizer (% w/w), representing between 5-50 times the target application rate. Synthetic compositions are blended and stored at either 22 °C with between 20-60% relative humidity or 30 °C with 80% relative humidity. The endophytes are reisolated from the synthetic compositions or seeds, on the day of treatment and each following period and the CFU recorded. Example 6. Assessment of improved plant characteristics: Vigor assay Assay of soybean seedling vigor [0128] Seed preparation: The lot quality of soybean seeds is first assessed by testing germination of 100 seeds. Seeds are placed, 8 seeds per petri dish, on filter paper in petri dishes, 12 ml of water is added to each plate and plates are incubated for 3 days at 24°C. The process should be repeated with a fresh seed lot if fewer than 95% of the seeds have germinated. One thousand soybean seeds are then surface sterilized by co-incubation with chlorine gas in a 20 x 30 cm container placed in a chemical fume hood for 16 hours. Percent germination of 50 seeds, per sterilization batch, is tested as above and confirmed to be greater than 95%. [0129] Preparation of endophyte treatments: Spore solutions are made by rinsing and scraping spores from agar slants which have been growing for about 1 month. Rinsing is done with 0.05% Silwet. Solutions are passed through Miracloth to filter out mycelia. Spores per ml are counted under a microscope using a hemocytometer. The stock suspension is then diluted into 10^6 spores/ml utilizing water.3 μl of spore suspension is used per soy seed (~10^3 CFUs/seed is obtained). Control treatments are prepared by adding equivalent volumes of sterile water to seeds. [0130] Assay of seedling vigor: Two rolled pieces of germination paper are placed in a sterile glass gar with 50 ml sterile water, then removed when completely saturated. Then the papers are separated and inoculated seeds are placed at approximately 1 cm intervals along the length of one sheet of moistened germination paper, at least 2.5 cm from the top of the paper and 3.8 cm from the edge of the paper. The second sheet of germination paper is placed on top of the soy seeds and the layered papers and seeds are loosely rolled into a tube. Each tube is secured with a rubber band around the middle, placed in a single sterile glass jar, and covered loosely with a lid. For each treatment, three jars with 15 seeds per jar are prepared. The position of jars within the growth chamber is randomized. Jars are incubated at 60% relative humidity, and 22°C day, 18°C night with 12 hours light and 12 hours dark for 4 days and then the lids are removed, and the jars incubated for an additional 7 days. Then the germinated soy seedlings are weighed and photographed, and root length and root surface area are measured. [0131] Dirt, excess water, seed coats and other debris are removed from seedlings to allow accurate scanning of the roots. Individual seedlings are laid out on clear plastic trays and trays are arranged on an Epson Expression 11000XL scanner (Epson America, Inc., Long Beach CA). Roots are manually arranged to reduce the amount of overlap. For root measurements, shoots are removed if the shape of the shoot causes it to overlap the roots. [0132] The WinRHIZO software version Arabidopsis Pro2016a (Regents Instruments, Quebec Canada) is used with the following acquisition settings: greyscale 4000 dpi image, speed priority, overlapping (1 object), Root Morphology: Precision (standard), Crossing Detection (normal). The scanning area is set to the maximum scanner area. When the scan is completed, the root area is selected, and root length and root surface area are measured. [0133] Statistical analysis is performed using R (R Core Team, 2016. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. R-project.org/) or a similar statistical software program. Assay of corn seedling vigor [0134] Seed preparation: The lot quality of corn seeds is first evaluated for germination by transfer of 100 seeds with 3.5 ml of water to a filter paper lined petri dish. Seeds are incubated for 3 days at 24°C. The process should be repeated with a fresh seed lot if fewer than 95% of the seeds have germinated. One thousand corn seeds are then surface sterilized by co-incubation with chlorine gas in a 20 x 30 cm container in a chemical fume hood for 12 hours. Percent germination of 50 seeds, per sterilization batch, is tested as above and confirmed to be greater than 95%. [0135] Optional reagent preparation: 7.5% PEG 6000 (Calbiochem, San Diego, CA) is prepared by adding 75 g of PEG to 1000 ml of water, then stirred on a warm hot plate until the PEG is fully dissolved. The solution is then autoclaved. [0136] Preparation of endophyte treatments: Spore solutions are made by rinsing and scraping spores from agar slants which have been growing for about 1 month. Rinsing is done with 0.05% Silwet. Solutions are passed through Miracloth to filter out mycelia. Spores per ml are counted under a microscope using a hemocytometer. The stock suspension is then diluted into 10^6 spores/ml utilizing water.3 μl of spore suspension is used per corn seed (~10^3 CFUs/seed is obtained). Control treatments are prepared by adding equivalent volumes of sterile water to seeds. [0137] Assay of seedling vigor: Either 25 ml of sterile water or, optionally, 25 ml of PEG solution as prepared above, is added to each CygTM germination pouch (Mega International, Newport, MN) and placed into pouch rack (Mega International, Newport, MN). Sterile forceps are used to place corn seeds prepared as above into every other perforation in the germination pouch. Seeds are fitted snugly into each perforation to ensure they do not shift when moving the pouches. Before and in between treatments forceps are sterilized using ethanol and flame and workspace wiped down with 70% ethanol. For each treatment, three pouches with 15 seeds per pouch are prepared. The germination racks with germination pouches are placed into plastic tubs and covered with perforated plastic wrap to prevent drying. Tubs are incubated at 60% relative humidity, and 22°C day, 18°C night with 12 hours light and 12 hours dark for 6 days to allow for germination and root length growth. Placement of pouches within racks and racks/tubs within the growth chamber is randomized to minimize positional effect. At the end of 6 days the corn seeds are scored manually for germination, root and shoot length. [0138] Statistical analysis is performed using R or a similar statistical software program. Assay of wheat seedling vigor [0139] Seed preparation: The lot of wheat seeds is first evaluated for germination by transfer of 100 seeds and with 8 ml of water to a filter paper lined petri dish. Seeds are incubated for 3 days at 24°C. The process should be repeated with a fresh seed lot if fewer than 95% of the seeds have germinated. Wheat seeds are then surface sterilized by co-incubation with chlorine gas in a 20 x 30 cm container in a chemical fume hood for 12 hours. Percent germination of 50 seeds, per sterilization batch, is tested as above and confirmed to be greater than 95%. [0140] Optional reagent preparation: 7.5% polyethylene glycol (PEG) is prepared by adding 75 g of PEG to 1000 ml of water, then stirring on a warm hot plate until the PEG is fully dissolved. The solution is then autoclaved. [0141] Preparation of endophyte treatments: Spore solutions are made by rinsing and scraping spores from agar slants which have been growing for about 1 month. Rinsing is done with 0.05% Silwet. Solutions are passed through Miracloth to filter out mycelia. Spores per ml are counted under a microscope using a hemocytometer. The stock suspension is then diluted into 10^6 spores/ml utilizing water.3 μl of spore suspension is used per wheat seed (~10^3 CFUs/seed was obtained). Seeds and spores are combined in a 50 ml falcon tube and gently shaken for 5-10 seconds until thoroughly coated. Control treatments are prepared by adding equivalent volumes of sterile water to seeds. [0142] Assay of seedling vigor: Petri dishes are prepared by adding four sheets of sterile heavy weight seed germination paper, then adding either 50 ml of sterile water or, optionally, 50 ml of PEG solution as prepared above, to each plate then allowing the liquid to thoroughly soak into all sheets. The sheets are positioned and then creased so that the back of the plate and one side wall are covered, two sheets are then removed and placed on a sterile surface. Along the edge of the plate across from the covered side wall 15 inoculated wheat seeds are placed evenly at least one inch from the top of the plate and half an inch from the sides. Seeds are placed smooth side up and with the pointed end of the seed pointing toward the side wall of the plate covered by germination paper. The seeds are then covered by the two reserved sheets, and the moist paper layers smoothed together to remove air bubbles and secure the seeds, and then the lid is replaced. For each treatment, at least three plates with 15 seeds per plate are prepared. The plates are then randomly distributed into stacks of 8-12 plates and a plate without seeds is placed on the top. The stacks are incubated at 60% relative humidity, and 22°C day, 18°C night with 12 hours light and 12 hours dark for 24 hours, then each plate is turned to a semi-vertical position with the side wall covered by paper at the bottom. The plates are incubated for an additional 5 days, then wheat seeds are scored manually for scored manually for germination, root and shoot length, root and shoot surface area, seedling mass, and seedling length. [0143] Statistical analysis is performed using R or a similar statistical software program. Assay of rice seedling vigor [0144] Seed preparation: The lot of rice seeds is first evaluated for germination by transfer of 100 seeds and with 8 ml of water to a filter paper lined petri dish. Seeds are incubated for 3 days at 24°C. The process should be repeated with a fresh seed lot if fewer than 95% of the seeds have germinated. Rice seeds are then surface sterilized by co-incubation with chlorine gas in a 20 x 30 cm container in a chemical fume hood for 12 hours. Percent germination of 50 seeds, per sterilization batch, is tested as above and confirmed to be greater than 95%. [0145] Optional reagent preparation: 7.5% polyethylene glycol (PEG) is prepared by adding 75 g of PEG to 1000 ml of water, then stirring on a warm hot plate until the PEG is fully dissolved. The solution is then autoclaved. [0146] Preparation of endophyte treatments: Spore solutions are made by rinsing and scraping spores from agar slants which have been growing for about 1 month. Rinsing was done with 0.05% Silwet. Solutions are passed through Miracloth to filter out mycelia. Spores per ml are counted under a microscope using a hemocytometer. The stock suspension is then diluted into 10^6 spores/ml utilizing water.3 μl of spore suspension is used per rice seed (~10^3 CFUs/seed was obtained). Seeds and spores are combined in a 50 ml falcon tube and gently shaken for 5-10 seconds until thoroughly coated. Control treatments are prepared by adding equivalent volumes of sterile water to seeds. [0147] Assay of seedling vigor: Petri dishes are prepared by adding four sheets of sterile heavy weight seed germination paper, then adding either 50 ml of sterile water or, optionally, 50 ml of PEG solution as prepared above, to each plate then allowing the liquid to thoroughly soak into all sheets. The sheets are positioned and then creased so that the back of the plate and one side wall are covered, two sheets are then removed and placed on a sterile surface. Along the edge of the plate across from the covered side wall 15 inoculated rice seeds are placed evenly at least one inch from the top of the plate and half an inch from the sides. Seeds are placed smooth side up and with the pointed end of the seed pointing toward the side wall of the plate covered by germination paper. The seeds are then covered by the two reserved sheets, and the moist paper layers smoothed together to remove air bubbles and secure the seeds, and then the lid is replaced. For each treatment, at least three plates with 15 seeds per plate are prepared. The plates are then randomly distributed into stacks of 8-12 plates and a plate without seeds is placed on the top. The stacks are incubated at 60% relative humidity, and 22°C day, 18°C night with 12 hours light and 12 hours dark for 24 hours, then each plate is turned to a semi-vertical position with the side wall covered by paper at the bottom. The plates are incubated for an additional 5 days, then rice seeds are scored manually for germination, root and shoot length. [0148] Statistical analysis is performed using R or a similar statistical software program. Example 7. Greenhouse assessment of improved plant characteristics under water deficit [0149] This example describes an exemplary method by which improved plant health of endophyte treated plants may be shown in a growth environment comprising a water deficit. Greenhouse assay setup: This greenhouse assay is conducted in individual plastic conetainers filled with soil. The soil-filled conetainers for the stress condition are not moistened. The soil-filled conetainers for the non-stress condition are thoroughly moistened by top watering with approximately 5 L of water as well as absorbing water from the bottom of the conetainers (approximately 3 L) for at least 1 hour prior to planting. Stress treatment containers are watered with 1L of water immediately before planting. An additional conetainer is prepared for each conetainer to be planted. These conetainers are filled with 30 cc of pea gravel. The soil-filled conetainers are each placed into a gravel filled conetainer (also referred to as a secondary conetainer). This greenhouse assay was conducted using soybean seeds treated with a commercial Bradyrhizobiym seed treatment and Bradyrhizobiym treated seeds are either coated with an endophyte synthetic composition or left untreated as untreated controls (lacking formulation and the one or more heterologously disposed endophyte) as described herein. Seeds are placed into each pot and lightly covered with potting soil. Replicated conetainers of each treatment and stress condition are placed in conetainer racks in a Latin square design. The trays of conetainers are lightly covered and placed in a growth chamber.48 hours after planting the covers are removed from the trays and all treatments are watered from the top with 1L of water. At 48 hours after planting the conetainer tray containing all treatments is watered from the bottom with 3.5L water, such that the water level just reaches the drain holes of the secondary conetainers; and the water level is maintained at this level throughout the experiment. Plants are harvested at 13-14 days post planting. The mass of the root tissue extending from the soil container is trimmed and weighted for each plant, and plant height is observed. Example 8. Greenhouse assessment of improved plant characteristics under nitrogen deficit [0150] This example describes an exemplary method by which improved plant health of endophyte treated plants may be shown in a growth environment comprising a nitrogen deficit. [0151] Greenhouse assay setup: This greenhouse assay is conducted in individual plastic pots, filled with moistened potting soil. This greenhouse assay is conducted using seeds (optionally, chemically treated) coated with one or more endophytes described herein and formulation control (lacking the one or more heterologously disposed endophytes) and untreated controls (lacking formulation and the one or more heterologously disposed endophyte) as described in Example 3. Seeds are placed onto each pot and lightly covered with potting soil. Replicated pots of each treatment are set up and placed on a greenhouse bench using a random block design. For example, 18 replicates are planted for each treatment and control. Nitrogen deficit is introduced by reducing the Nitrogen in the Hoagland’s solution (3 mM N), which is used to water the plants. Plants are monitored daily for emergence and watered as necessary to maintain a moist but not saturated soil surface (for example, plants are watered with 125 ml Hoagland’s solution (3 mM N) per pot on every Monday, Wednesday, and Friday). [0152] The following growth and vigor metrics are collected for each treatment: percentage emergence at Day 4, 5, 7 (for soybean, winter wheat and cotton) or Day 3, 4, 5 (for corn), leaf count (the number of fully expanded leaves on the main stem) at Days 10, 17 and 24. [0153] Additional vigor and growth metrics may be collected including shoot height, leaf area, number of chlorotic leaves, chlorophyll content, number of live leaves, etc. At harvest, plants are gently removed from pots, washed with tap water to remove dirt, and photographed. Plant tissue is collected for nutrient composition analysis. Plants are put into a paper bag and dried in an oven. Optionally, the plant is separated into shoot and root tissue prior to drying. The dry weight of each individual plant, or shoot or root thereof, is recorded. Example 9. Greenhouse assessment of improved plant characteristics under phosphorus deficit [0154] This example describes an exemplary method by which improved plant health of endophyte treated plants may be shown in a growth environment comprising a phosphorus deficit. [0155] This greenhouse assay is conducted in individual plastic pots, filled with moistened potting soil. This greenhouse assay is conducted using seeds (optionally, chemically treated) coated with one or more endophytes described herein and formulation control (lacking the one or more heterologously disposed endophytes) and untreated controls (lacking formulation and the one or more heterologously disposed endophyte) as described in Example 4. Seeds are placed onto each pot and lightly covered with potting soil. Replicated pots of each treatment are set up and placed on a greenhouse bench using a random block design. For example, 16 replicates are planted for each treatment and control. Phosphorus deficit is introduced by removing Phosphorus from the Hoagland’s solution (0 mM P), which is used to water the plants. Plants are monitored daily for emergence and watered as necessary to maintain a moist but not saturated soil surface (for example, plants are watered with 125 ml Hoagland’s solution (0 mM P) per pot on every Monday, Wednesday, and Friday). [0156] The following growth and vigor metrics are collected for each treatment: percentage emergence at Day 4, 5, 7 (for soybean, winter wheat and cotton) or Day 3, 4, 5 (for corn), leaf count (the number of fully expanded leaves on the main stem) at Days 10, 17 and 24. [0157] Additional vigor and growth metrics may be collected including shoot height, leaf area, coloration of leaves, number of live leaves, etc. At harvest, plants are gently removed from pots, washed with tap water to remove dirt, and photographed. Plant tissue is collected for nutrient composition analysis. Plants are put into a paper bag and dried in an oven. Optionally, the plant is separated into shoot and root tissue prior to drying. The dry weight of each individual plant, or shoot or root thereof, is recorded. Example 10. Greenhouse assessment of improved plant health under biotic stress [0158] This example describes an exemplary method by which improved plant health of endophyte treated plants was shown in a growth environment comprising the crop pathogen Pythium ultimum or Rhizoctonia solani. This assay utilized cotton, soybeans, winter wheat, or corn. [0159] Preparation of pathogen inoculum A stock of Rhizoctonia solani anastomosis group 4 or Pythium ultimum var. ultimum is grown on a standard potato dextrose agar plate. Plugs of fresh mycelium were then transferred into standard potato dextrose broth. After sufficient growth was achieved, the culture was poured though cheesecloth to capture the fungal biomass, which was subsequently rinsed with water. After removing excess rinsate, a roughly equivalent volume of water was added to the fungal biomass before blending to create a slurry. The resulting slurry was further diluted to the required concentration necessary to observe desired level of symptoms. [0160] Greenhouse assay setup The greenhouse assay was conducted in a commercial potting soil. A divot was placed in the center of a pot containing wetted soil using a standardized dibble. An appropriate volume of slurry was added to the center of each divot. An equivalent volume of water was added for control treatments. [0161] This greenhouse assay was conducted using seeds coated with one or more endophytes described herein in PBS and untreated controls (lacking endophyte). Seeds were placed onto each divot after addition of the inoculum. The seeds were then covered with uninoculated soil and again watered. High soil moisture levels were maintained throughout the course of the experiment. Fourteen replicates were included in a randomized design to obtain sufficient statistical power for analysis, this assay was repeated. Plants were grown in a controlled environment until 7 days post emergence of control plants. Shoot fresh weight was measured on a per plant basis. Table 5. Plant phenotypes under biotic stress of endophyte-treated and control-treated plants T reatment Metric Value Value Treatment D escriptor Concentration Crop Condition MIC-54924 Shoot Fresh 58.01 percent change 1E6 cells/mL Cotton Pythium Weight untreated control ultimum MIC-54924 Shoot Fresh - percent change 0.05 OD Cotton Pythium Weight 52.71 untreated control ultimum MIC-54924 Shoot Fresh 14.85 percent change 0.05 OD Cotton Pythium Weight untreated control ultimum MIC-54924 Shoot Fresh 36.38 percent change 3.33E7 Soy Pythium Weight untreated control cells/mL ultimum Treatment Metric Value Value Treatment D escriptor Concentration Crop Condition MIC-54924 Shoot Fresh 34.39 percent change 1E6 cells/mL Soy Pythium Weight untreated control ultimum MIC-54924 Shoot Fresh 54.01 percent change 3.33E7 Soy Pythium Weight untreated control CFU/mL ultimum MIC-54924 Shoot Fresh 2.65 percent change 3.33E7 Soy Pythium Weight untreated control cells/mL ultimum MIC-54924 Shoot Fresh - percent change 3.33E7 Soy Pythium Weight 30.02 untreated control cells/mL ultimum MIC-54924 Shoot Fresh - percent change 3.33E7 Soy Pythium Weight 34.08 untreated control cells/mL ultimum MIC-54924 Shoot Fresh 2.05 percent change 3.33E7 Soy Pythium Weight untreated control cells/mL ultimum MIC-54924 Shoot Fresh 34.84 percent change 10^6 Soy Pythium Weight untreated control ultimum MIC-54924 Shoot Fresh - percent change 3.33E7 Soy Pythium Weight 11.39 untreated control CFU/mL ultimum MIC-54924 Shoot Fresh 64.45 percent change 1E6 cells/mL Winter Pythium Weight untreated control Wheat ultimum MIC-54924 Shoot Fresh 225.1 percent change 3.33E7 Winter Pythium Weight 8 untreated control CFU/mL Wheat ultimum MIC-54924 Shoot Fresh 17.79 percent change 3.33E7 Winter Pythium Weight untreated control cells/mL Wheat ultimum MIC-54924 Shoot Fresh 59.96 percent change 3.33E7 Winter Pythium Weight untreated control cells/mL Wheat ultimum MIC-54924 Shoot Fresh 54.9 percent change 3.33E7 Winter Pythium Weight untreated control CFU/mL Wheat ultimum MIC-54924 Shoot Fresh -3.3 percent change 3.33E7 Corn Rhizoctonia Weight untreated control cells/mL solani MIC-54924 Shoot Fresh 12.18 percent change 6.66E7 Corn Rhizoctonia Weight untreated control cells/mL solani MIC-54924 Shoot Fresh - percent change 3.33E7 Corn Rhizoctonia Weight 24.49 untreated control cells/mL solani MIC-54924 Shoot Fresh - percent change 0.05 OD Cotton Rhizoctonia Weight 52.27 untreated control solani MIC-54924 Shoot Fresh - percent change 3.33E7 Soy Rhizoctonia Weight 13.59 untreated control cells/mL solani MIC-54924 Shoot Fresh 53.46 percent change 3.33E7 Soy Rhizoctonia Weight untreated control cells/mL solani MIC-54924 Shoot Fresh 8.24 percent change 3.33E7 Soy Rhizoctonia Weight untreated control CFU/mL solani MIC-54924 Shoot Fresh 1.73 percent change 10^6 Soy Weight untreated control MIC-54924 Shoot Fresh 37.84 percent change 3.33E7 Soy Weight untreated control cells/mL MIC-54924 Shoot Fresh - percent change 3.33E7 Soy Weight 26.04 untreated control CFU/mL MIC-54924 Shoot Fresh - percent change 3.33E7 Winter Weight 57.56 untreated control cells/mL Wheat Treatment Metric Value Value Treatment D escriptor Concentration Crop Condition MIC-54924 Shoot Fresh - percent change 10^6 Winter Rhizoctonia Weight 40.11 untreated control Wheat solani MIC-54924 Shoot Fresh 91.55 percent change 3.33E7 Winter Rhizoctonia Weight untreated control cells/mL Wheat solani Table 6. Summary of plant phenotypes under biotic stress growth chamber testing Average percent Number change from Win Treatment Crop Pathogen Metric of runs untreated control Rate MIC-54924 Corn Pythium Shoot Fresh 2 4.11 50% aphanidermatum Weight MIC-54924 Corn Rhizoctonia Shoot Fresh 5 -5.58 40% solani Weight MIC-54924 Cotton Pythium Shoot Fresh 3 6.43 67% ultimum Weight MIC-54924 Cotton Rhizoctonia Shoot Fresh 1 -51.55 0% solani Weight MIC-54924 Soybean Pythium Shoot Fresh 9 10.49 67% ultimum Weight MIC-54924 Soybean Rhizoctonia Shoot Fresh 7 13.62 71% solani Weight MIC-54924 Winter Pythium Shoot Fresh 8 101.27 100% Wheat ultimum Weight MIC-54924 Winter Rhizoctonia Shoot Fresh 5 18.47 50% Wheat solani Weight Example 11. Greenhouse assessment of improved plant health under biotic stress [0162] This example describes an exemplary method by which improved plant health of endophyte treated plants were shown in a growth environment comprising the crop pathogen Fusarium sp., one of the causal agents of seedling damping off disease. This assay may utilize dicots or monocots, including, for example, soybean or wheat. [0163] Preparation of Fusarium sp. inoculum A stock of Fusarium sp. was grown on a standard potato dextrose agar plate. Plugs of fresh mycelium were then transferred into breathable bag containing a sterile mixture of water and grain such as sorghum or millet. After sufficient growth was achieved, the culture was removed from the bags and dried. After drying the biomass was coarsely ground. [0164] Greenhouse assay setup The greenhouse assay was conducted in a media mixture consisting of a commercial potting soil and a minimum of 50% inert inorganic material such as calcined clay or vermiculite or pearlite. An appropriate volume of ground pathogen was added to the soil mixture to obtain desired level of symptoms. [0165] This greenhouse assay was conducted using seeds coated with one or more endophytes described herein in PBS formulation and untreated controls (lacking endophyte). A seed was added to the surface of the infested media. The seed was then covered with media lacking pathogen and again watered. High soil moisture levels were maintained throughout the course of the experiment. Fourteen replicates were included in a randomized design to obtain sufficient statistical power for analysis; this assay was repeated. Shoot fresh weight was measured on a per plant basis. Table 7. Plant phenotypes under biotic stress (Fusarium oxysporum) of endophyte-treated and control- treated plants Treatment Measurement Treatment Metric Value Value Descriptor Concentration Crop Time MIC-54924 Shoot Fresh 28.33 percent change 3.33E7 Soybeans 11 days after Weight untreated control cells/mL planting MIC-54924 Shoot Fresh - percent change 1E6 cells/mL Soybeans 10 days after Weight 48.85 untreated control planting MIC-54924 Shoot Fresh - percent change 3.33E7 Soybeans 11 days after Weight 10.06 untreated control cells/mL planting MIC-54924 Shoot Fresh 8.88 percent change 3.33E8 Soybeans 11 days after Weight untreated control cells/mL planting MIC-54924 Shoot Fresh 4.91 percent change 3.33E7 Soybeans 11 days after Weight untreated control cells/mL planting MIC-54924 Shoot Fresh 45.51 percent change 3.33E6 Soybeans 11 days after Weight untreated control cells/mL planting MIC-54924 Shoot Fresh - percent change 1E6 cells/mL Winter 7 days after Weight 10.33 untreated control Wheat planting MIC-54924 Shoot Fresh 27.32 percent change 3.33E7 Winter 8 days after Weight untreated control cells/mL Wheat planting MIC-54924 Shoot Fresh 5.11 percent change 3.33E7 Winter 8 days after Weight untreated control cells/mL Wheat planting MIC-54924 Shoot Fresh 35.29 percent change 3.33E7 Winter 8 days after Weight untreated control cells/mL Wheat planting MIC-54924 Shoot Fresh 55.39 percent change 3.33E7 Winter 8 days after Weight untreated control cells/mL Wheat planting MIC-54924 Shoot Fresh 17.7 percent change 3.33E6 Winter 9 days after Weight untreated control cells/mL Wheat planting Table 8. Summary of results of Fusarium growth chamber testing Average percent Number of change from Win Treatment Crop Pathogen Metric runs untreated control Rate MIC- Corn Fusarium Shoot Fresh 4 11.68 75% 54924 graminearum Weight Average percent Number of change from Win Treatment Crop Pathogen Metric runs untreated control Rate MIC- Soybean Fusarium Shoot Fresh 6 4.49 67% 54924 oxysporum Weight MIC- Soybean Fusarium Shoot Fresh 6 5.20 67% 54924 virguliforme Weight MIC- Winter Fusarium Shoot Fresh 3 5.08 33% 54924 Wheat graminearum Weight MIC- Winter Fusarium Shoot Fresh 16 15.68 81% 54924 Wheat oxysporum Weight Example 12. In vitro Assessment of Production of Antibiotic Metabolites Using Live Endophyte Cultures [0166] This example describes an exemplary method by which microbes may be shown to inhibit the growth of hyphal phytopathogens in vitro. Such phytopathogens can be members of the “true” fungi, phylum Eumycota, or from other taxonomic groups with a similar growth habit such as members of the phylum Oomycota. Hyphal growth can be described as organism growth along thread-like structures composed of connected cells. Such growth is found commonly among fungi and oomycetes, and even some genera of bacteria. In this assay, the hyphal growth should be in a roughly uniform, radial manner. This assay is comprised of a Petri plate containing an agar-based media and a hyphal phytopathogen grown concomitantly a live endophyte. This assay is completed using pathogens: Cercospora sojina, Colletotrichum truncatum, Corynespora cassicola, Diaporthe aspalathi, Fusarium graminearum, Macrophomina phaseolina, Pythium debaryanum, Rhizoctonia solani, and Sclerotinia sclerotiorum. [0167] Preparation of Hyphal Phytopathogen A Petri plate containing a media suitable for the growth of the target hyphal pathogen was inoculated with the target hyphal pathogen. The initial inoculum should be from an axenic culture, but non-axenic cultures containing stable endophytes may also be used. Any media may be used that supports healthy growth of the hyphal pathogen. After inoculation on the media-containing Petri plate, the culture was allowed to grow until reaching the edge of the Petri plate. A test pathogen sample was collected from this plate. [0168] Preparation of the test sample The microbial samples for testing, also referred to as test samples, were MIC-54924 in water dispersed formulation at three dosages: 0.5 mL/kg seeds, 1.3 mL/kg seeds, and 2 mL/kg seeds. Seeds treated with a commercial chemical fungicide (“Chemical Control”), and untreated controls were also prepared. Viable soybean seeds were used in these assays. [0169] Alternatively, a liquid culture of either type of microbe can be grown, and viable material is removed by various methods including, but not limited to, filtration or autoclaving. This later method of testing a non-viable test sample is best used when the test microbe displays a much faster rate of radial growth than the hyphal pathogen being tested. This later method is also more sensitive at differentiating between the passive production of antimicrobial metabolites versus an active biological process such a mycophagy. Test samples from all these methods can also be applied to viable or devitalized seeds. Seed application replicates conditions a microbe might experience when used as a seed treatment, having physical and biochemical interactions with seed material. [0170] Assay Set-Up A Petri dish containing a solid agar test media (herein referred to as the test plate) was obtained. A sterile instrument was used to remove a test pathogen plug from the hyphal pathogen plate culture described in Preparation of Hyphal Phytopathogen. This test pathogen plug was placed on a fresh solid agar plate. Next a test sample was applied to the test plate at a distance such that the test sample and test plate came into physical contact after more than one day of growth. Four treated seeds were placed on each plate approximately equidistant from the pathogen plug. Alternately, live microbe in formulation or fermentation media was applied directly to the test plate, or for assaying a non-viable test sample, an agar plug was removed from the test plate using a sterile instrument to create a well to hold the test sample. The well was then filled with the non-viable test sample, and the sample was absorbed into the agar media. [0171] Use of Multiple Growth Media. This plate-based assay was repeated with multiple media types. Medias were chosen to vary important growth inputs such as carbon source, presence and concentration of various salts, and presence of extracts from different plant species or organs. Fig.8, 9, and 10 show examples of use of differing media (King’s medium B agar and yeast extract peptone dextrose agar), where the assays were also run without selected carbon sources (glycerol in Fig.8 and 9, dextrose in Fig.11). MIC-54924 showed a greater zone of inhibition of fungal pathogens when in nutrient scarce conditions. MIC- 54924 showed enhanced inhibition of Pythium ultimum and Fusarium graminearum in the absence of glycerol. MIC-54924 showed enhanced inhibition of Rhizoctonia solani in the absence of glycerol. [0172] Assessment After setting up, hyphal pathogens were allowed to grow for sufficient time such that the hyphal front met or just passed the test sample and analyzed at 2, 4, 6, 8, 10, and 12 days post plating. Plates were scored based on the degree of restriction of growth of the hyphal front around the test sample, clearing around the test sample, and comparison of the morphology of the hyphal pathogen near the test sample to areas away from the test sample. When anti-pathogen metabolites were not produced and secreted, the hyphal pathogen grew over the test sample with little to no visible effect on growth. Table 9. Summary of observations of plate-based anti-biosis assays with MIC-54924 treated seeds Observations of MIC-54924 treated seeds Cercospora Significant reduction in pathogen colony diameter relative to untreated controls at all dosages sojina at 4, 6, 8, 10, and 12 days post plating. Colony diameters of pathogen colonies on plates containing seeds treated with MIC-54924 were smaller than those on plates containing Chemical Control treated seeds at days 6, 8, 10, and 12 post plating. Significant reduction in pathogen colony diameter relative to untreated controls at all dosages Colletotrichum at 2, 4, 8, and 10 days post plating. truncatum Corynespora Significant reduction in pathogen colony diameter relative to untreated controls at all dosages cassicola at 4, 6, 8, 10, and 12 days post plating. Colony diameters of pathogen colonies on plates containing seeds treated with MIC-54924 were smaller than those on plates containing Chemical Control treated seeds at days 6, 8, 10, and 12 post plating. Diaporthe Significant reduction in pathogen colony diameter relative to untreated controls at all dosages aspalathi at 2, 4, and 6 days post plating. Fusarium Significant reduction in pathogen colony diameter relative to untreated controls at all dosages graminearum at 4 days post plating. Significant reduction in pathogen colony diameter relative to untreated controls at all dosages Macrophomina at 4 days post plating. phaseolina Pythium No significant reduction in pathogen colony diameters on any days. debaryanum Rhizoctonia Significant reduction in pathogen colony diameter relative to untreated controls at all dosages solani at 2. Significant reduction at day 4 for the 0.5 mL/kg and 1.3 mL/kg dosages, but not the 2 mL/kg dosage. Significant reduction at day 6 for the 2 mL/kg dosage, but not the 0.5 mL/kg and 1.3 mL/kg dosages. Sclerotinia No significant reduction in pathogen colony diameters on any days. sclerotiorum Example 13. In vitro Assessment of Production of Antibiotic Metabolites Using Filtered or Dead Endophyte Cultures [0173] This example describes an exemplary method by which microbes may be shown to produce metabolites that inhibit the growth of hyphal phytopathogens in vitro. Such phytopathogens can be members of the “true” fungi, phylum Eumycota, or from other taxonomic groups with a similar growth habit such as members of the phylum Oomycota. Hyphal growth can be described as organism growth along thread-like structures composed of connected cells. Such growth is found commonly among fungi and oomycetes, and even some genera of bacteria. In this assay, the hyphal growth should be in a roughly uniform, radial manner. This assay is comprised of a Petri plate containing an agar-based media and a hyphal phytopathogen grown in the presence of the spent media from a previously grown endophyte. [0174] Preparation of Hyphal Phytopathogen A Petri plate containing a media suitable for the growth of the target hyphal pathogen is inoculated with the target hyphal pathogen. The initial inoculum should be from an axenic culture, but non-axenic cultures containing stable . multiple ways. The test sample can be added directly to the molten media prior to the plates being poured. The test sample can be applied to the surface of an already prepared solid agar test plate, spread evenly over the surface with excess liquid allowed to dry. Finally, an agar plug can be removed from the test plate using a sterile instrument to create a well to hold the test sample. The well is then filled with a test sample, and the sample is absorbed into the agar media. In addition to the test sample, a sterile instrument is used to remove a test pathogen plug from the hyphal pathogen plate culture and placed on the test plate. Alternatively, a spore or hyphal slurry from the phytopathogen can be applied to the test plate, either as a drop or spread evenly over the top of the plate with excess liquid allowed to dry. [0177] Use of Multiple Growth Media Pathogens grown under various environmental conditions are expected to show differential sensitivity to those metabolites. For this reason, this assay is performed on multiple media types. Medias are chosen to vary important growth inputs such as carbon source, presence and concentration of various salts, and presence of extracts from different plant species or organs. [0178] Assessment If applied as a hyphal plug or drop of slurry, hyphal pathogens are allowed to grow for sufficient time such that the hyphal front meets or just passes the test sample. In cases where anti-pathogen metabolites are produced and secreted, a restriction of growth of the hyphal front around the test sample is commonly observed. Often this will also result in an area of clearing around the test sample. In these cases, the morphology of the hyphal pathogen near the test sample will often also be dissimilar from areas away from the test sample. Alternatively, when anti-pathogen metabolites are not produced and secreted, the hyphal pathogen will grow over the test sample with little to no visible effect on growth. [0179] If phytopathogen was applied across the surface of the plate, reduced growth can be observed microscopically shortly after application by looking at the germination of spores. An area of decreased pathogen growth around the test sample may also be observed in some embodiments of this assay. A relative decrease in transparency compared to a control plate may also be observed when the test sample is applied to the molten media or uniformly across the surface of the test plate. Example 14. Nematode Egg Inoculum Preparation [0180] This example describes an exemplary method for obtaining nematode eggs for use in stock population maintenance, in planta screening assays, and for hatching for in vitro assays. The nematode species utilized are Meloidogyne incognita (Southern root-knot nematode, “RKN”), Heterodera glycines (Soybean cyst nematode, “SCN”), and Rotylenchulus reniformis (Reniform nematode, “REN”). Populations of nematodes may be obtained, for example from a stock crop of corn for RKN, cotton for REN, and soybean for SCN. [0181] Experimental Preparation Eggs are extracted from nematode stock crops; RKN and REN are collected from plants that are ~60-75 days old, and SCN is collected from plants that are ~70-85 days old. The above ground biomass is removed and discarded. If multiples are being extracted, necessary precautions are taken to prevent cross contamination of nematode species. [0182] RKN and REN Egg Extraction from Roots Soil is washed from the roots of infected stock crops and the roots are placed in a prepared container. To extract the nematodes, a 0.625 % NaOCl solution is added to the container and the roots are agitated for 4 minutes using an orbital shaker set at approximately 100-120 rpm. [0183] The NaOCl extraction solution is then poured through an 8” diameter 25 ^m pore sieve with an 8” diameter 75 ^m pore sieve stacked on top to sift out debris. The roots are manually scrubbed over the sieve stack while running water over them. Alternately the roots are placed in a blender with water and pulsed until macerated. If using a blender, the contents are poured back through the sieve stack. The 75 ^m pore sieve is rinsed into the 25 ^m pore sieve. Eggs are captured on the 25 ^m pore sieve. The 25 ^m pore sieve is held at an angle and gently rinsed with water to collect all the eggs into a small pool at the bottom. The eggs are carefully collected into a storage container using a wash bottle. [0184] SCN Cyst Extraction from Soil. Soil is washed from the roots of infected stock crops and the excess soil and rinse water are collected in a small bucket. The roots are manually scrubbed to remove cysts that remain visibly stuck to the roots. Eight-inch sieves are stacked on top of a separate small bucket. An 850 ^m pore sieve is on top and a 250 ^m pore sieve is underneath. The collected soil and rinse water are mixed and then allowed to settle for 3 seconds before the liquid portion of the soil mixture is poured through the sieve stack. Water is added to the retained soil, and the mixing, settling, and pouring steps are repeated. After the second wash the remaining soil is discarded. [0185] The 850 ^m pore sieve is rinsed into the 250 ^m pore sieve. Cysts are captured on the 250 ^m pore sieve. The 250 ^m pore sieve is held at an angle and gently rinsed with water to collect all the eggs into a small pool at the bottom. The cysts are carefully collected into a storage container using a wash bottle, using a minimal amount of water. [0186] SCN Egg Extraction from Cysts Collected cysts are placed into a mortar, and thoroughly ground using a pestle. An 8” 75 ^m pore sieve is stacked on top of an 8” 25 ^m pore sieve and the mortar contents are washed through the sieves. The eggs are collected from the 25 ^m pore sieve by rinsing the 75 ^m pore sieve into the 25 ^m pore sieve. Eggs are captured on the 25 ^m pore sieve. The 25 ^m pore sieve is held at an angle and gently rinsed with water to collect all the eggs into a small pool at the bottom. The eggs are carefully collected into a storage container using a wash bottle. The cyst mixture remaining on the 75^m pore sieve is collected again and the grinding, sieving, and rinsing steps are repeated until the cysts are extracted. [0187] Egg Centrifugation Eggs are further separated from small debris by centrifugation with sucrose. A sucrose solution is made by adding 495 g of white cane sugar into a 1L bottle and filling up to the 1L measurement with DI water. The mixture is stored at 4 ל C until ready to use. Approximately 25 ml of sucrose solution is added to each 50 ml conical tube. Then the egg inoculum is mixed to evenly distribute eggs and the inoculum poured into the prepared conical tubes until the total inoculum volume is distributed. The tubes are then centrifuged at 1040 rpm for 1 minute. Nematode eggs float at the top of the solution in the centrifuged tubes. A sieve stack is made using 3” diameter sieves, with a 75 ^m pore sieve on top of a 25 ^m pore sieve. The top half of the tube contents is poured though the sieves and rinsed with water to wash away the sugar solution. The eggs are collected from the 25 ^m pore sieve by rinsing the 75 ^m pore sieve into the 25 ^m pore sieve. Eggs are captured on the 25 ^m pore sieve. The 25 ^m pore sieve is held at an angle and gently rinsed with water to collect all the eggs into a small pool at the bottom. The eggs are carefully collected into a storage container using a wash bottle. The eggs are enumerated at 40 × magnification using an inverted microscope. Eggs to be used for in planta screening are standardized to 2000 eggs/mL. Example 15. In-vitro Nematode Supernatant Assay [0188] RKN and SCN eggs are collected as described above. A hatching environment is prepared by lining a small sterile container with a clean, wood fiber based delicate task tissue and saturating the tissue with deionized water. The collected eggs are mixed with a sugar solution and centrifuged at 240 g for one minute. The supernatant containing the eggs is poured through a 25 ^m pore sieve. Approximately 250,000 to 500,000 eggs are added to the prepared hatching environment, and the hatching environment is incubated at 30 ל C and shaken at 25 rpm in the dark. Deionized water is added to the hatching environment to ensure the water level does not fall to below the tissue, and at least every 3 days to ensure proper oxygenation. After 6 days, hatched second stage juveniles (J2) are rinsed through a stack of 45 ^m and 25 ^m sieves that have been previously sprayed with 70% ethanol and rinsed with deionized water. Sterilized deionized water is used to collect J2 from the 45 ^m pore sieve into a sterile 100 mL glass beaker, and J2 concentration standardized to 30 േ 5 per 10 ^L with sterile deionized water. A control treatment is prepared by adding 2 ^L abamectin to 78 ^L of sterile deionized water per replicate. [0189] In-vitro Supernatant Screening Protocol Ten ^L of the prepared J2 suspension is added to wells of a 96well half area plate. One abamectin control is added to each plate. Additionally, one negative control (media lacking endophyte) is prepared for each plate. Sterilized deionized water and then endophyte supernatant (Total volume: 80 ^L) are aliquoted in each treatment well of the 96-well plate to desired supernatant percentage. In a fume hood, 80 ^L of the prepared abamectin is added into active wells of Control Plate.10 ^L of propidium iodide (0.2 mM) is added to reach a final concentration of 20 ^M. Total well volume should equal 100 ^L. Plates are sealed with a breathable membrane and stored in the dark at room temperature for 48 hours. [0190] Intensity of propidium iodide in each well is measured using the propidium iodide filter on a BioTek Cytation 5 Cell Imaging Multimode Reader (Agilent, Santa Clara, CA, USA). The intensity of propidium iodide (which binds to dead cells) is proportional to the mortality of the incubated nematodes. Example 16. Greenhouse assessment of improved plant health under biotic stress (soybean cyst nematode) [0191] This example describes an exemplary method by which improved plant health of endophyte treated plants may be shown in a growth environment comprising the crop pest soybean cyst nematode (Heterodera glycines). [0192] Greenhouse assays are conducted using soybean seeds (optionally, chemically treated soybean seeds) coated with one or more of the endophytes described herein and formulation control (lacking the one or more heterologously disposed endophytes) and untreated controls (lacking formulation and the one or more heterologously disposed endophyte). Microbe treated soybean seeds are planted, infected with nematodes, maintained, and phenotyped in grow rooms. [0193] In one embodiment, the following method is used.98 cones are placed in each conetainer to obtain the needed number of conetainers. Masks are placed over cones and cones are filled with soil. The conetainer is placed in a deep pan and water is added until the soil in the cones is saturated. Two soybean seeds are planted 2.5 cm deep in each conetainer. [0194] One ml containing 2,000 H. glycines eggs is pipetted into each cone at planting or the desired number of days after planting. Seedlings are thinned to one per cone after emergence and watered as appropriate. [0195] Phenotyping is performed as follows. The height of each plant is measured, e.g., by placing the ruler on the lip of a cell and measuring the plant’s height to the nearest millimeter. The mass of each plant is measured, e.g., by cutting the plant at the soil surface, placing the shoot in the weighing container, allowing the weight to stabilize, and autorecording the mass via the scale’s software. The number of H. glycines cysts may be counted after extraction from soybean roots as described herein. The water suspension containing 150 cm^3 of soil is poured through nested 75-^m and 25-^m-pore sieves to extract vermiform stages (juveniles and males). Vermiform stages are collected on the 75-^m-pore sieve and centrifuged using, e.g., the sucrose centrifugation-flotation method. Example 17. Greenhouse assessment of improved plant health under biotic stress (soybean aphid) [0196] This example describes an exemplary method by which improved plant health of endophyte treated plants may be shown in a growth environment comprising the crop pest soybean aphid (Aphis glycines). [0197] Greenhouse assays are conducted using soybean seeds (optionally, chemically treated soybean seeds) coated with one or more of the endophytes described herein and formulation control (lacking the one or more heterologously disposed endophytes) and untreated controls (lacking formulation and the one or more heterologously disposed endophyte) as described in herein. Microbe treated soybean seeds are planted, infected with soybean aphids (Aphis glycines), maintained in grow rooms, and phenotyped. [0198] In one embodiment, the following method is used.98 cones are placed in each conetainer to obtain the needed number of conetainer. Masks are placed over cones and cones are filled with potting medium or soil. The conetainer is placed in a deep pan and water is added until the soil in the cones is saturated. One soybean seed is planted in each conetainer. Each conetainer is placed in a growth tub and watered. [0199] A community of soybean aphids is maintained on a stock of soybean plants. To prepare for infestation of the experimental plants, leaves are removed from infested soybean plants from the stock community. One or more leaves are examined under a stereoscope to make sure the aphids are alive and vigorous. Infested leaf cutlets are placed in square plates to keep leaves alive until the treatment plants are infested with aphids. In some embodiments, 20 infested leaf cutlets are used per each 98-cone tray used in the experiment. The infested leaf cutlets are introduced to the growth environment of the experimental plants at planting or the desired number of days after planting, in some embodiments, 9 days after planting. The experimental conetainers are infested following an infestation pattern to allow for aphid choice feeding in planta. The infested experimental plants are maintained in their growth environment until phenotyping. [0200] The plants may be phenotyped at one or more times after infestation, for example 1 day, 4 days, 7 days or more after infestation. Measurement of one or more traits of agronomic importance is performed as follows. The height of each plant is measured, e.g., by placing the ruler on the lip of a cell and measuring the plant’s height to the nearest millimeter or using an automated tool such as a Phenospex PlantEye 3D laser scanner (Phenospex B.V., Heerlen, The Netherlands). Other traits of agronomic importance may be measured either manually or using a tool such as the Phenospex PlantEye 3D laser scanner, for example the greenness of the plants and the leaf and/or above ground plant area. The mass of each plant may be measured for example via destructive sampling, e.g., by cutting the plant at the soil surface, placing the shoot in the weighing container, allowing the weight to stabilize, and autorecording the mass via the scale’s software. The experimental plants may be maintained through their reproductive stages, and traits of agronomic importance such as number of flowers, number of pods and number of seeds per pod may be measured. Example 18. Field assessment of improved plant health of soy under biotic stress [0201] This example describes an exemplary method by which improved plant health of endophyte treated plants were shown in a growth environment comprising Pythium or Rhizoctonia. This assay utilized soybeans and wheat. [0202] Field trials were conducted using soybean or winter wheat seeds coated with one or more of the endophytes described herein and controls (untreated). Replicate plots were planted per endophyte or control treatment in a randomized complete block design. Each plot consisted of an approximately 7.62 m (25 ft.) by 0.76 m (2.5 ft.) row. The following growth metrics were measured: early emergence, full emergence, plant height, root weight, shoot weight, and yield. [0203] At the end of the field trial employing endophyte treatment and control treatment plants, plants were randomly dug out from each row, kept in a plastic bag, and brought back to lab for metric measurements. For each seedling, shoot and root were separated by cutting the seedling 3 cm from the first branch of the root. The heights of the separated shoot of each plant were measured, followed by fresh shoot weight, and fresh root weight. The main root was vertically split into two halves and discoloration of xylem is scored. [0204] Summary statistics are generated using ggplot2 package of R (R Core Team, 2016. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. R-project.org/). Table 10. Plant phenotypes under biotic stress of endophyte-treated plants in field conditions values represent % change relative to controls. Condition Treatment Metric Value Crop Condition Descriptor MIC- Early -7.3 Soy Pythium inoculated field 54924 Emergence trial MIC- Full Emergence 3.3 Soy Pythium inoculated field 54924 trial MIC- Plant Height 4 Soy Pythium inoculated field 54924 trial MIC- Root Weight 32.4 Soy Pythium inoculated field 54924 trial MIC- Shoot Weight 12.3 Soy Pythium inoculated field 54924 trial MIC- Yield 0.5 Soy Pythium inoculated field 54924 trial MIC- Early -4.09 Soy Rhizoctonia inoculated field 54924 Emergence trial MIC- Full Emergence 38.9 Soy Rhizoctonia inoculated field 54924 trial MIC- Plant Height -4.8 Soy Rhizoctonia inoculated field 54924 trial MIC- Root Weight 0 Soy Rhizoctonia inoculated field 54924 trial MIC- Shoot Weight -2.4 Soy Rhizoctonia inoculated field 54924 trial MIC- Yield -0.8 Soy Rhizoctonia inoculated field 54924 trial Table 11. Plant phenotypes under biotic stress of endophyte-treated plants in field conditions, values represent % change relative to untreated controls. Condition Treatment Metric Value Crop Condition Descriptor MIC- Early 23.4 Winter Pythium inoculated field 54924 Emergence Wheat trial MIC- Full 7.3 Winter Pythium inoculated field 54924 Emergence Wheat trial [0205] Seven trials were conducted at locations in the United States inoculated with Fusarium graminearum in 2022. [0206] Field trials were conducted using soybean seeds coated with MIC-54924 plus sedaxane, mefenoxam, and fludioxonil (“MIC-54924 + Chem."), seeds were also treated with sedaxane, mefenoxam, and fludioxonil without an endophyte (“Chemical Control”), and untreated controls were also prepared. Replicate plots were planted per endophyte or control treatment in a randomized complete block design. Each plot consisted of an approximately 1.5 m by 10 m row. The following growth metrics were measured: root fresh weight, full emergence, plant height, and yield. Table 12. Plant phenotypes under biotic stress of endophyte-treated plants in field conditions, values represent % change relative to untreated controls. Win rate Condition Treatment Metric Value Crop Condition Descriptor MIC-54924 + Root Fresh 1.2% Soybean Fusarium inoculated Chem. Weight graminearum field trial Chemical Root Fresh 6.1% Soybean Fusarium inoculated Control Weight graminearum field trial MIC-54924 + Full 21.3% 96% Soybean Fusarium inoculated Chem. Emergence graminearum field trial Chemical Full 23.9% 93% Soybean Fusarium inoculated Control Emergence graminearum field trial MIC-54924 + Plant Height 2.6% Soybean Fusarium inoculated Chem. graminearum field trial Chemical Plant Height 0.04% Soybean Fusarium inoculated Control graminearum field trial MIC-54924 + Yield 4.3% 86% Soybean Fusarium inoculated Chem. graminearum field trial Chemical Yield 2.5% 71% Soybean Fusarium inoculated Control graminearum field trial Example 19. Field assessment of improved plant health under biotic stress [0207] This example describes an exemplary method by which improved plant health of endophyte treated plants were shown. Wheat [0208] Sixteen trials were conducted at 11 field locations under naturally occurring disease Argentina. Target diseases included Dreschera tritici repentis, Bipolaris sorokiniana, Fusarium graminearum, Rizhoctonia solani and Phyti m sp. [0209] Field trials were conducted using wheat seeds coated with MIC-54924 in water dispersed formulation at three dosages: 0.45 mL/kg seeds, 0.65 mL/kg seeds, and 1 mL/kg seeds, seeds treated with a commercial chemical fungicide (“Chemical Control”), and untreated controls. Replicate plots were planted per endophyte or control treatment in a randomized complete block design. Each plot consisted of an a proximately 1.5 m by 10 m row. The following growth metrics were measured: early emergence, emergence at 10 days after emergence, full emergence, NDVI, heads per square meter prior to harvest, and yield. Table 13. Plant phenotypes under biotic stress of endophyte-treated plants in field conditions under naturally occurring disease pressure, values represent % change relative to controls. Condition Treatment Metric Value Crop Condition Descriptor MIC-54924 Early 6.75 Wheat Naturally 0.45 mL/kg dose emergence occurring disease MIC-54924 Early 5.38 Wheat Naturally 0.65 mL/kg dose emergence occurring disease MIC-54924 Early -0.91 Wheat Naturally 1 mL/kg dose emergence occurring disease Chemical Early 4.92 Wheat Naturally control emergence occurring disease MIC-54924 10 days after 9.99 Wheat Naturally 0.45 mL/kg dose emergence occurring disease MIC-54924 10 days after 6.41 Wheat Naturally 0.65 mL/kg dose emergence occurring disease MIC-54924 10 days after -0.41 Wheat Naturally 1 mL/kg dose emergence occurring disease Chemical 10 days after 1.92 Wheat Naturally control emergence occurring disease MIC-54924 Full 6.06 Wheat Naturally 0.45 mL/kg dose emergence occurring disease MIC-54924 Full 4.17 Wheat Naturally 0.65 mL/kg dose emergence occurring disease MIC-54924 Full -0.83 Wheat Naturally 1 mL/kg dose emergence occurring disease Chemical Full 4.85 Wheat Naturally control emergence occurring disease MIC-54924 NDVI – 2.57 Wheat Naturally 0.45 mL/kg dose tillering stage occurring disease MIC-54924 NDVI – 1.76 Wheat Naturally 0.65 mL/kg dose tillering stage occurring disease MIC-54924 NDVI – 1.62 Wheat Naturally 1 mL/kg dose tillering stage occurring disease Chemical NDVI – 0.63 Wheat Naturally control tillering stage occurring disease MIC-54924 NDVI – flag 1.02 Wheat Naturally 0.45 mL/kg dose leaf stage occurring disease MIC-54924 NDVI – flag 2.47 Wheat Naturally 0.65 mL/kg dose leaf stage occurring disease MIC-54924 NDVI – flag 1.12 Wheat Naturally 1 mL/kg dose leaf stage occurring disease Chemical NDVI – flag 3.33 Wheat Naturally control leaf stage occurring disease Table 14. Plant phenotypes under biotic stress of endophyte-treated plants in field conditions under naturally occurring disease pressure, values number of heads of wheat per square meter. g e g e e r . measured: early emergence (0-2 days after beginning of emergence) and full emergence (approximately 10 days after the early emergence count). Sites were classified as stressed when the chemical control provided significant improvement or >5% improvement in full emergence relative to the untreated control. MIC-54924 treated seeds had an average of 1.94% increase in percent full emergence relative to untreated controls in the Fusarium inoculated trials. MIC-54924 treated seeds had an in dispersed formulation at three dosages: 0.50 mL/kg seeds, 1.3 mL/kg seeds, and 2 mL/kg seeds, seeds treated with a commercial chemical fungicide (“Chemical Control”), and d l R li l l d d h l i d sease MIC-54924 Early -2.11 Soybean Naturally 1.3 mL/kg emergence occurring dose disease MIC-54924 Early -4.38 Soybean Naturally 2 mL/kg dose emergence occurring disease Chemical Early -2.35 Soybean Naturally Control emergence occurring disease MIC-54924 Full 1.0 Soybean Naturally 0.50 mL/kg emergence occurring dose disease MIC-54924 Full 2.34 Soybean Naturally 1.3 mL/kg emergence occurring dose disease MIC-54924 Full 0.23 Soybean Naturally 2 mL/kg dose emergence occurring disease Condition Treatment Metric Value Crop Condition Descriptor Chemical Full 3.13 Soybean Naturally Control emergence occurring disease MIC-54924 Yield 1.41 Soybean Naturally 0.50 mL/kg occurring dose disease MIC-54924 Yield 0.14 Soybean Naturally 1.3 mL/kg occurring dose disease MIC-54924 Yield 1.58 Soybean Naturally 2 mL/kg dose occurring disease Chemical Yield 1.24 Soybean Naturally Control occurring disease Table 17. Plant phenotypes under biotic stress of endophyte-treated plants in field conditions (2H2021), values represent % change relative to controls. Condition Treatment Metric Value Crop Condition Descriptor MIC-54924 Early 7.20 Soybean Naturally 0.50 mL/kg emergence occurring dose disease MIC-54924 Early 2.06 Soybean Naturally 1.3 mL/kg emergence occurring dose disease ld conditions under kg/hectare. on Treatement Descriptor lly 0.5 mL/kg dose ase lly 1.3 mL/kg dose occurring disease MIC-54924 Yield 148.69 Soybean Naturally 2 mL/kg dose occurring disease h i l l Yi l 1 11 lly ase lly ase culated with Fusarium crophomina Rhizoctonia solani. IC-54924 in water ds, 1.3 mL/kg seeds, e (“Chemical endophyte or control of 24 square meters. l emergence. ergence relative to and in field conditions ignificant increases in ing Rhizoctonia solani lly occurring disease n. Two locations um and F. 54924 in water eeds, and 2 mL/kg yte or control treatment in a randomized complete block design. Each plot consisted of an approximately 1.5 m by 10 m row. The following growth metrics were measured: early emergence, full emer ence and ield MIC- Full 4.48 Corn Fusarium 0.55 mL/kg 54924 emergence inoculated dose MIC- Full -5.37 Corn Fusarium 1.3 mL/kg 54924 emergence inoculated dose MIC- Full -3.41 Corn Fusarium 2 mL/kg dose 54924 emergence inoculated MIC- Yield 7.3 Corn Fusarium 0.55 mL/kg 54924 inoculated dose MIC- Yield 10.3 Corn Fusarium 1.3 mL/kg 54924 inoculated dose MIC- Yield 11.1 Corn Fusarium 2 mL/kg dose 54924 inoculated MIC- Yield 1.03 Corn Low incidence 0.55 mL/kg 54924 natural disease dose MIC- Yield 4.03 Corn Low incidence 1.3 mL/kg 54924 natural disease dose MIC- Yield 4.87 Corn Low incidence 2 mL/kg dose 54924 natural disease Example 20. Field assessment of improved plant health [0219] This example describes an exemplary method by which improved plant health of endophyte treated plants may be shown. [0220] Field trials are conducted using seeds (e.g. cotton, soy, corn, wheat, etc. optionally chemically treated) coated with one or more of the endophytes described herein and formulation control (lacking the one or more heterologously disposed endophytes) and untreated controls (lacking formulation and the one or more heterologously disposed endophyte) as described herein. The following growth metrics are measured: percent emergence at 14 days post planting, standing count at 28 and 45 days post planting, plant vigor at 14, 28, and 45 days post planting, plant height at 45 days post planting, fresh shoot weight, fresh root weight, disease rating at a 0-3 scale (3 denotes strong disease symptoms) using the split-root scoring system at 45 days post planting, nematode count at 45 days post planting, and yield parameters. 0221 S mm r t ti ti r n r t d in l t2 k f R (R Core Team, 2016. R: or Statistical omponent: Fat erein. Analysis of fat cial Agricultural Analysis of AOAC its entirety. Samples rs. using a Soxlhet red gravimetrically. and control (seed omponent: Ash erein. Analysis of ash cial Agricultural Analysis of AOAC International, 20th Edition (2016). Samples are weighed into pre-weighed crucibles, and ashed in a furnace at 600ºC for 3hr. Weight loss on ashing is calculated as % ash. Mean percent changes between the treatment (one or more heterologously disposed endophytes) and control (lacking the one or more heterologously disposed endophytes) with the formulation but no endophyte are calculated Example 23. Method of determining seed nutritional quality trait component: Fiber [0224] Seed samples from harvested plants are obtained as described herein. Analysis of fiber is conducted on replicate samples according to the Association of Official Agricultural Chemists Reference Method AOAC 920.39, of the Official Methods of Analysis of AOAC International, 20th Edition (2016). Samples are weighed into filter paper, defatted and dried, and hydrolyzed first in acid, then in alkali solution. The recovered portion is dried, weighed, ashed at 600ºC, and weighed again. The loss on ashing is calculated as % Fiber. Mean percent changes between the treatment (one or more heterologously disposed endophytes) and control (lacking the one or more heterologously disposed endophytes) with the formulation but no endophyte are calculated. Example 24. Method of determining seed nutritional quality trait component: Moisture [0225] Seed samples from harvested plants are obtained as described herein. Analysis of moisture is conducted on replicate samples according to the Association of Official Agricultural Chemists Reference Method AOAC 920.39, of the Official Methods of Analysis of AOAC International, 20th Edition (2016). Samples are weighed into pre-weighed aluminum dishes and dried at 135ºC for 2hrs. Weight loss on drying is calculated as % Moisture. Mean percent changes between the treatment (one or more heterologously disposed endophytes) and control (lacking the one or more heterologously disposed endophytes) with the formulation but no endophyte are calculated. Example 25. Method of Determining Seed Nutritional Quality Trait Component: Protein [0226] Seed samples from harvested plants are obtained as described herein. Analysis of protein is conducted on replicate samples according to the Association of Official Agricultural Chemists Reference Method AOAC 920.39, of the Official Methods of Analysis of AOAC International, 20th Edition (2016). Samples are combusted and nitrogen gas is measured using a combustion nitrogen analyzer (Dumas). Nitrogen is multiplied by 6.25 to calculate % Protein. Mean percent changes between the treatment (one or more heterologously disposed endophytes) and control (lacking the one or more heterologously disposed endophytes) with the formulation but no endophyte) are calculated. Example 26. Method of determining seed nutritional quality trait component: Carbohydrate [0227] Seed samples from harvested plants are obtained as described herein. Analysis of carbohydrate is determined for replicate samples as a calculation according to the following formula: Total Carbohydrate = 100% - % (Protein + Ash + Fat + Moisture + Fiber), where % Protein is determined according to the method described herein, % Ash is determined according to the method described herein, % Fat is determined according to the method described herein, % Moisture is determined according to the method described herein, and % Fiber is determined according to the method described herein. Mean percent changes between the treatment (one or more heterologously disposed endophytes) and control (lacking the one or more heterologously disposed endophytes) are calculated. Example 27. Method of determining seed nutritional quality trait component: Calories [0228] Seed samples from harvested plants are obtained as described herein. Analysis of Calories is determined for replicate samples as a calculation according to the following formula: Total Calories = (Calories from protein) + (Calories from carbohydrate) + Calories from fat), where Calories from protein are calculated as 4 Calories per gram of protein (as determined according to the method described herein), Calories from carbohydrate are calculated as 4 Calories per gram of carbohydrate (as determined according to the method described herein), and Calories from fat are calculated as 9 Calories per gram of fat (as determined according to the method described herein). Mean percent changes between the treatment (one or more heterologously disposed endophytes) and control (lacking the one or more heterologously disposed endophytes) are calculated. Example 28. Blotter paper assessment of improved plant health under biotic stress (Cercospora kikuchii) [0229] This example describes an exemplary method by which improved plant health of endophyte treated plants may be shown in a growth environment comprising the crop pathogens: Cercospora kikuchii, Fusarium spp., and Phomopsis sp. [0230] Assays were conducted using soybean seeds coated with MIC-54924 and untreated controls (lacking formulation and the one or more heterologously disposed endophyte). Three doses of MIC-54924 in water dispersed formulation were applied: 0.5 mL inoculant per kg seed, 1.3 inoculant per kg seed, 2 inoculant per kg seed. Treated soybean seeds and untreated controls were placed on damp blotter paper inoculated with the fungal pathogen and incubated at 24 degrees Celsius for 8 days with 12 hours of light/dark. Eight replicated sets of 50 seeds per treatment or control were prepared. Seeds were scored for visual incidence of pathogen infection. Treatment with MIC-54924 at 1.3 ml inoculant /kg seed resulted in a significant reduction of incidence of Cercospora kikuchii on the treated seeds compared to untreated controls. Treatment with MIC-54924 at 0.5 mL/kg, 1.3 mL /kg, and 2 mL/kg resulted in a significant reduction of incidence of Fusarium spp. on the treated seeds compared to untreated controls. Treatment with MIC-54924 at all resulted in reduction of incidence of Phomopsis sp. on the treated seeds compared to untreated controls, with especially significant results in the 0.5 mL/kg, 1.3 mL /kg dosages. Table 20. Observed incidence of C. kikuchii in blotter paper assessment Treatment Average % incidence C. kikuchii MIC-54924 @ 0.5 ml/kg 11.5 MIC-54924 @ 2 ml/kg 11 Untreated 11 MIC-54924 @ 1.3 ml/kg 5.25 Example 29. Analysis of antifungal peptide W1 The structures of sequence variants of the antifungal peptide W1 identified in MIC-54924 (SEQ IDs.98 and 99) were compared to antifungal protein W1 identified in marine Bacillus amyloliquefaciens (Wen et al. Front. Microbiol., 2022 Volume 13). A predicted protein structures was generated for Bacillus amyloliquefaciens W1 and MIC-54924 W1 SEQ ID.98 (“MIC-54924 W1-98”) and MIC-54924 W1 SEQ ID.99 (“MIC-54924 W1-99”) using PEP- FOLD3 (Lamiable et al. Nucleic Acids Res.2016 Jul 8; 44(Web Server issue): W449– W454). TM-SCORE is a scoring function to assess the similarity of protein structures (Y. Zhang, J. Skolnick, Proteins, 57: 702-710 (2004). A TM-score greater than 0 and less than 0.17 indicates random structural similarity, and a TM-score of greater than 0.5 and less than 1 indicates structures expected to have similar structure (Yang Zhang and Jeffrey Skolnick, Proteins 200457: 702-710). The TM-SCORE of MIC-54924 W1-99 and Bacillus amyloliquefaciens W1was TM-score was 0.49. Comparison of predicted structures MIC- 54924 W1-99 and Bacillus amyloliquefaciens W1 (Fig.11) and MIC-54924 W1-98 and Bacillus amyloliquefaciens W1 (Fig.12) shows the presence of a kink in the MIC-54924 W1- 98 and MIC-54924 W1-99 structures at the amino terminus of the structure, indicated by the arrow labeled “A” in Fig.11 and Fig.12.^ Table 21. Amino acid variants of MIC-54924 W1. SEQID Variant Sequence 1 00 W1 peptide (SEQ ID: 99), L4F MGVFQNIWPLLLMFVIFYFLLIRPQ 1 01 W1 peptide (SEQ ID: 99), L4F N6Q MGVFQQIWPLLLMFVIFYFLLIRPQ 1 02 W1 peptide (SEQ ID: 99), L4F Q5N MGVFNNIWPLLLMFVIFYFLLIRPQ 1 03 W1 peptide (SEQ ID: 99), L4F Q5N N6Q MGVFNQIWPLLLMFVIFYFLLIRPQ 1 04 W1 peptide (SEQ ID: 99), L4F Q5N N6Q W8Y MGVFNQIYPLLLMFVIFYFLLIRPQ 1 05 W1 peptide (SEQ ID: 99), L4F Q5N W8Y MGVFNNIYPLLLMFVIFYFLLIRPQ 1 06 W1 peptide (SEQ ID: 99), L4F W8Y MGVFQNIYPLLLMFVIFYFLLIRPQ 1 07 W1 peptide (SEQ ID: 99), L4M MGVMQNIWPLLLMFVIFYFLLIRPQ MGVMQQIWPLLLMFVIFYFLLIRPQ MGVMNNIWPLLLMFVIFYFLLIRPQ MGVMNQIWPLLLMFVIFYFLLIRPQ MGVMNQIYPLLLMFVIFYFLLIRPQ MGVMNNIYPLLLMFVIFYFLLIRPQ 1 13 W1 peptide (SEQ ID: 99), L4M W8Y MGVMQNIYPLLLMFVIFYFLLIRPQ 1 14 W1 peptide (SEQ ID: 99), L4V MGVVQNIWPLLLMFVIFYFLLIRPQ 1 15 W1 peptide (SEQ ID: 99), L4V N6Q MGVVQQIWPLLLMFVIFYFLLIRPQ 1 16 W1 peptide (SEQ ID: 99), L4V Q5N MGVVNNIWPLLLMFVIFYFLLIRPQ 1 17 W1 peptide (SEQ ID: 99), L4V Q5N N6Q MGVVNQIWPLLLMFVIFYFLLIRPQ 1 18 W1 peptide (SEQ ID: 99), L4V Q5N N6Q W8Y MGVVNQIYPLLLMFVIFYFLLIRPQ 1 19 W1 peptide (SEQ ID: 99), L4V Q5N W8Y MGVVNNIYPLLLMFVIFYFLLIRPQ 1 20 W1 peptide (SEQ ID: 99), L4V W8Y MGVVQNIYPLLLMFVIFYFLLIRPQ 1 21 W1 peptide (SEQ ID: 99), N6Q MGVLQQIWPLLLMFVIFYFLLIRPQ 1 22 W1 peptide (SEQ ID: 99), N6Q W8Y MGVLQQIYPLLLMFVIFYFLLIRPQ 1 23 W1 peptide (SEQ ID: 99), Q5N MGVLNNIWPLLLMFVIFYFLLIRPQ 1 24 W1 peptide (SEQ ID: 99), Q5N N6Q MGVLNQIWPLLLMFVIFYFLLIRPQ 1 25 W1 peptide (SEQ ID: 99), Q5N N6Q W8Y MGVLNQIYPLLLMFVIFYFLLIRPQ 1 26 W1 peptide (SEQ ID: 99), Q5N W8Y MGVLNNIYPLLLMFVIFYFLLIRPQ 1 27 W1 peptide (SEQ ID: 99), W8Y MGVLQNIYPLLLMFVIFYFLLIRPQ 1 28 W1 peptide (SEQ ID: 98), Q1N NNIWPLLLMFVIFYFLLIRPQ 1 29 W1 peptide (SEQ ID: 98), Q1N W4Y NNIYPLLLMFVIFYFLLIRPQ 1 30 W1 peptide (SEQ ID: 98), Q1N N2Q NQIWPLLLMFVIFYFLLIRPQ 1 31 W1 peptide (SEQ ID: 98), Q1N N2Q W3Y NQIYPLLLMFVIFYFLLIRPQ 1 32 W1 peptide (SEQ ID: 98), W3Y QNIYPLLLMFVIFYFLLIRPQ SEQID Variant Sequence