Title:
ENZYMATIC MUTANT SUITABLE FOR HOMOGENEOUS IMMUNOASSAY METHOD
Document Type and Number:
WIPO Patent Application WO/2020/162203
Kind Code:
A1
Abstract:
Provided is a β glucuronidase mutant suitable for the homogeneous immunoassay method. This β glucuronidase mutant is characterized in that methionine at position 516 in the amino acid sequence of β glucuronidase of E. coli is substituted with lysine, and tyrosine at position 517 is unsubstituted or substituted with an aromatic amino acid other than tyrosine.
Inventors:
UEDA HIROSHI (JP)
SU JIULONG (JP)
KITAGUCHI TETSUYA (JP)
OHMURO YUKI (JP)
SU JIULONG (JP)
KITAGUCHI TETSUYA (JP)
OHMURO YUKI (JP)
Application Number:
PCT/JP2020/002264
Publication Date:
August 13, 2020
Filing Date:
January 23, 2020
Export Citation:
Assignee:
TOKYO INST TECH (JP)
International Classes:
C12N9/24; C07K19/00; C12N15/62; C12Q1/34
Domestic Patent References:
| WO2017130610A1 | 2017-08-03 |
Foreign References:
| US20120252071A1 | 2012-10-04 | |||
| JP2002502363A | 2002-01-22 | |||
| JP2017534055A | 2017-11-16 |
Other References:
GEDDIE, M. L. ET AL.: "Antibody-induced oligomerization and activation of an engineered reporter enzyme", JOURNAL OF MOLECULAR BIOLOGY, vol. 369, 2007, pages 1052 - 1059, XP022083580, DOI: 10.1016/j.jmb.2007.03.076
FLORES, H. ET AL.: "Increasing the thermal stability of an oligomeric protein, beta- glucuronidase", JOURNAL OF MOLECULAR BIOLOGY, vol. 315, 2002, pages 325 - 337, XP004469310, DOI: 10.1006/jmbi.2001.5223
SU , J. ET AL.: "Creation of stable and strictly regulated enzyme switch for signal-on immunodetection of various small antigens", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 128, no. 6, 21 June 2019 (2019-06-21), pages 677 - 682, XP085902667, DOI: 10.1016/j.jbiosc.2019.05.015
FLORES, H. ET AL.: "Increasing the thermal stability of an oligomeric protein, beta- glucuronidase", JOURNAL OF MOLECULAR BIOLOGY, vol. 315, 2002, pages 325 - 337, XP004469310, DOI: 10.1006/jmbi.2001.5223
SU , J. ET AL.: "Creation of stable and strictly regulated enzyme switch for signal-on immunodetection of various small antigens", JOURNAL OF BIOSCIENCE AND BIOENGINEERING, vol. 128, no. 6, 21 June 2019 (2019-06-21), pages 677 - 682, XP085902667, DOI: 10.1016/j.jbiosc.2019.05.015
Attorney, Agent or Firm:
NOMURA, Kenichi et al. (JP)
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