Title:
FAST METHOD FOR DETECTING MICRO-ORGANISMS IN FOOD SAMPLES
Document Type and Number:
WIPO Patent Application WO2004106547
Kind Code:
A3
Abstract:
The present invention relates to a specific method accomplishing fast and specific identification of contaminating micro-organisms in large amounts of food stuffs. A method has been developed based on random genome fragments or Zipcode oligonucleotides and DNA microarray technology that overcomes the disadvantages of whole-genome DNA-DNA hybridisation. In particular, the present invention provides a method for characterising micro-organisms possibly present in a sample, comprising the steps of collecting said micro-organisms if present, extracting nucleic acids from said micro-organisms, specifically amplifying said nucleic acids, thereby providing an amplified nucleic acid mixture comprising the target nucleic acid in amplified form, and analysing the amplified nucleic acid mixture, whereby the said micro-organisms if present are characterised. The present invention further relates to the use of filters, microarrays and amplification steps in said method as well as a kit comprising the same.
Inventors:
ANDREOLI PETER (NL)
THIJSSEN JOOST (NL)
ANTHONY RICHARD (NL)
VOS PIETER (NL)
DE LEVITA WOUTER (NL)
THIJSSEN JOOST (NL)
ANTHONY RICHARD (NL)
VOS PIETER (NL)
DE LEVITA WOUTER (NL)
Application Number:
PCT/EP2004/005951
Publication Date:
March 17, 2005
Filing Date:
June 02, 2004
Export Citation:
Assignee:
CHECK POINTS BV (NL)
ANDREOLI PETER (NL)
THIJSSEN JOOST (NL)
ANTHONY RICHARD (NL)
VOS PIETER (NL)
DE LEVITA WOUTER (NL)
ANDREOLI PETER (NL)
THIJSSEN JOOST (NL)
ANTHONY RICHARD (NL)
VOS PIETER (NL)
DE LEVITA WOUTER (NL)
International Classes:
C12Q1/68; C12Q1/70; (IPC1-7): C12Q1/68
Domestic Patent References:
| WO2002043937A2 | 2002-06-06 |
Foreign References:
| US20020182598A1 | 2002-12-05 |
Other References:
BLOHM D.H. AND GUISEPPI-ELIE A.: "New developments in microarray technology", CURRENT OPINION IN BIOTECHNOLOGY, vol. 12, no. 1, February 2001 (2001-02-01), pages 41 - 47, XP008040446, ISSN: 0958-1669
GERRY N P ET AL: "Universal DNA microarray method for multiplex detection of low abundance point mutations", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 292, no. 2, 17 September 1999 (1999-09-17), pages 251 - 262, XP004462280, ISSN: 0022-2836
BUSTI ELENA ET AL: "Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction).", BMC MICROBIOLOGY [ELECTRONIC RESOURCE]. ENGLAND 20 SEP 2002, vol. 2, no. 1, 20 September 2002 (2002-09-20), pages 27, XP002262041, ISSN: 1471-2180, Retrieved from the Internet [retrieved on 20031119]
SMALL JACK ET AL: "Direct detection of 16S rRNA in soil extracts by using oligonucleotide microarrays", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 67, no. 10, October 2001 (2001-10-01), pages 4708 - 4716, XP002245295, ISSN: 0099-2240
19 November 2003, CEPARATION, HELMOND, THE NETHERLANDS, XP002262042
MIKHAILOVICH V ET AL: "Identification of rifampin-resistant mycobacterium tuberculosis strains by hybridization, PCR and ligase detection reaction on oligonucleotide microchips", JOURNAL OF CLINICAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 39, no. 7, July 2001 (2001-07-01), pages 2531 - 2540, XP002970585, ISSN: 0095-1137
GERRY N P ET AL: "Universal DNA microarray method for multiplex detection of low abundance point mutations", JOURNAL OF MOLECULAR BIOLOGY, LONDON, GB, vol. 292, no. 2, 17 September 1999 (1999-09-17), pages 251 - 262, XP004462280, ISSN: 0022-2836
BUSTI ELENA ET AL: "Bacterial discrimination by means of a universal array approach mediated by LDR (ligase detection reaction).", BMC MICROBIOLOGY [ELECTRONIC RESOURCE]. ENGLAND 20 SEP 2002, vol. 2, no. 1, 20 September 2002 (2002-09-20), pages 27, XP002262041, ISSN: 1471-2180, Retrieved from the Internet
SMALL JACK ET AL: "Direct detection of 16S rRNA in soil extracts by using oligonucleotide microarrays", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 67, no. 10, October 2001 (2001-10-01), pages 4708 - 4716, XP002245295, ISSN: 0099-2240
19 November 2003, CEPARATION, HELMOND, THE NETHERLANDS, XP002262042
MIKHAILOVICH V ET AL: "Identification of rifampin-resistant mycobacterium tuberculosis strains by hybridization, PCR and ligase detection reaction on oligonucleotide microchips", JOURNAL OF CLINICAL MICROBIOLOGY, WASHINGTON, DC, US, vol. 39, no. 7, July 2001 (2001-07-01), pages 2531 - 2540, XP002970585, ISSN: 0095-1137
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