Title:
FLUORESCENCE IMAGE REGISTRATION METHOD, GENE SEQUENCING INSTRUMENT AND SYSTEM, AND STORAGE MEDIUM
Document Type and Number:
WIPO Patent Application WO/2020/155043
Kind Code:
A1
Abstract:
The present invention provides a fluorescence image registration method, comprising: acquiring a fluorescence image of a biochip; selecting a preset local region of the fluorescence image; acquiring the position of a minimum value of the sum of brightness values of pixels in a first direction and a second direction, and obtaining pixel-level registration points; dividing the pixel-level registration points into non-defective pixels and defective pixels according to a preset classification rule; determining whether the fluorescence image meets a preset standard according to the number of the non-defective pixels, and if the fluorescence image meets the preset standard, correcting the positions of the defective pixels according to the positions of the non-defective pixels; acquiring the position of the center of gravity of image points of fluorescent molecules according to the center of gravity method; fitting straight lines in the first direction and the second direction respectively according to the position of the center of gravity of the image points of fluorescent molecules, the cross point of the straight lines being sub-pixel level positions of the registration points; and acquiring boundary points of the fluorescence image and calculating the positions of the boundary points. The embodiments of the present invention further provide a gene sequencing system, a gene sequenator, and a storage medium. The embodiments of the present invention can optimize the positioning and alignment operation of the fluorescent molecules in the fluorescence image.
Inventors:
SHEN JINJIN (CN)
LI DAWEI (CN)
LIU YANGBAO (CN)
FENG GE (CN)
LI MEI (CN)
LI YUXIANG (CN)
LI DAWEI (CN)
LIU YANGBAO (CN)
FENG GE (CN)
LI MEI (CN)
LI YUXIANG (CN)
Application Number:
PCT/CN2019/074244
Publication Date:
August 06, 2020
Filing Date:
January 31, 2019
Export Citation:
Assignee:
BGI SHENZHEN (CN)
International Classes:
G06T7/30; G06T7/38
Foreign References:
| CN105427327A | 2016-03-23 | |||
| CN108537830A | 2018-09-14 | |||
| CN105408939A | 2016-03-16 | |||
| CN104992433A | 2015-10-21 |
Attorney, Agent or Firm:
SHENZHEN SCIENBIZIP INTELLECTUAL PROPERTY AGENCY CO., LTD. (CN)
Download PDF:
Previous Patent: KEYBOARD
Next Patent: CONVOLUTION CALCULATION DEVICE AND METHOD, PROCESSOR AND MOVABLE DEVICE
Next Patent: CONVOLUTION CALCULATION DEVICE AND METHOD, PROCESSOR AND MOVABLE DEVICE