Login| Sign Up| Help| Contact|

Patent Searching and Data


Title:
GENE ANALYSIS SYSTEM
Document Type and Number:
WIPO Patent Application WO/2016/162997
Kind Code:
A1
Abstract:
In order to interpret an arbitrary sequence region in many genes contained in many cells, it is needed to digest a nucleic acid into fragments and then introduce a sequence that is different from one cell to another cell into each of the fragments. However, in the conventional constitutions for analyzing many cells, there is a problem that the contamination by digested fragments among zones may occur before the introduction of a tag sequence inherent in each of the zones. The present invention provides a system for capturing a nucleic acid extracted from a cell in each of multiple zones on a substrate and then synthesizing complementary DNA (cDNA) to the nucleic acid in each of the zones, said system being equipped with a means for immediately introducing a tag sequence inherent in each of the zones into a reaction product.

Inventors:
TANABE MAIKO (JP)
SHIRAI MASATAKA (JP)
SAKAI TOMOYUKI (JP)
Application Number:
PCT/JP2015/061077
Publication Date:
October 13, 2016
Filing Date:
April 09, 2015
Export Citation:
Click for automatic bibliography generation   Help
Assignee:
HITACHI LTD (JP)
International Classes:
C12Q1/68; C12M1/00; C12N15/09
Domestic Patent References:
WO2014020657A12014-02-06
WO2011068088A12011-06-09
WO2003035841A22003-05-01
Foreign References:
US20140378317A12014-12-25
JP2009284834A2009-12-10
US20140315729A12014-10-23
Other References:
LUO YACUI ET AL.: "ICP-MS-Based Multiplex and Ultrasensitive Assay of Viruses with Lanthanide-Coded Biospecific Tagging and Amplification Strategies", ANAL. CHEM., vol. 85, no. 20, 2013, pages 9428 - 9432, XP055322037
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Download PDF:
View/Download PDF      PDF Help



 
Previous Patent: NON-CONTACT POWER-FEEDING SYSTEM

Next Patent: THERAPY PLANNING APPARATUS AND PARTICLE RADIATION THERAPY APPARATUS