Title:
GENETICALLY MODIFIED BACTERIA PRODUCING THREE DNA REPAIR ENZYMES AND METHOD FOR THE EVALUATION OF DNA REPAIR ACTIVITY
Document Type and Number:
WIPO Patent Application WO/2020/047637
Kind Code:
A8
Abstract:
The present invention provides three recombinant E. coli strains for the production of the enzymes CPD-photolyase, 6,4-bifunctional photolyase and 6,4-photolyase, from bacterial Antarctic isolates of the genus Hymenobacter the first one and Sphingomonas the others. Also provides the process of production and purification of the recombinant enzymes with high performance, high degree of purity and high catalytic repair activity, having applications in, but it is not limited to, cosmetics and pharmaceutical industry. Other aspect of this invention is to provide a fast, cheap and qualitative method for the determination of the CPD-photolyase activity.
Inventors:
MARIZCURRENA JUAN JOSÉ (UY)
CASTRO SOWINSKI SUSANA (UY)
CASTRO SOWINSKI SUSANA (UY)
Application Number:
PCT/BR2019/050373
Publication Date:
October 01, 2020
Filing Date:
September 04, 2019
Export Citation:
Assignee:
UNIV LA REPUBLICA (UY)
DE CENCO NASCIMENTO MARIA CLAUDIA (BR)
MINISTERIO DE DEFENSA NAC INSTITUTO ANTARTICO URUGUAYO (UY)
DE CENCO NASCIMENTO MARIA CLAUDIA (BR)
MINISTERIO DE DEFENSA NAC INSTITUTO ANTARTICO URUGUAYO (UY)
International Classes:
C12N9/88; C12N1/21; C12N15/60; C12N15/70; C12Q1/527; C12Q1/683; C12R1/19; C12R1/20
Attorney, Agent or Firm:
DAVID DO NASCIMENTO ADVOGADOS ASSOCIADOS et al. (BR)
Download PDF: