REFERENCE TO SEQUENCE LISTING AND INCORPORATION BY REFERENCE THEREOF The present application includes a Sequence Listing in electronic format, filed pursuant to PCT Administrative Instructions 801-806 on a single CD-R disc, in triplicate, containing a file named pto_HBL100. txt, created 24 January 2001, having 11, 029, 597 bytes. The Sequence Listing contained in said file on said disc is incorporated herein by reference in its entirety.

Field of the Invention The present invention relates to genome-derived single exon microarrays useful for verifying the expression of regions of genomic DNA predicted to encode protein. In particular, the present invention relates to unique genome-

derived single exon nucleic acid probes expressed in human HBL 100 cells and single exon nucleic acid microarrays that include such probes.

Background of the Invention For almost two decades following the invention of general techniques for nucleic acid sequencing, Sanger et al., Proc. Natl. Acad. Sci. USA 70 (4) : 1209-13 (1973) ; Gilbert et al., Proc. Natl. Acad. Sci. USA 70 (12) : 3581-4 (1973), these techniques were used principally as tools to further the understanding of proteins-known or suspected-about which a basic foundation of biological knowledge had already been built. In many cases, the cloning effort that preceded sequence identification had been both informed and directed by that antecedent biological understanding.

For example, the cloning of the T cell receptor for antigen was predicated upon its known. or suspected cell type-specific expression, by its suspected membrane association, and by the predicted assembly of its gene via T cell-specific somatic recombination. Subsequent sequencing efforts at once confirmed and extended understanding of this family of proteins. Hedrick et al., Nature 308 (5955) : 153-8 (1984).

More recently, however, the development of high throughput sequencing methods and devices, in concert with large public and private undertakings to sequence the human and other genomes, has altered this investigational paradigm : today, sequence information often precedes understanding of the basic biology of the encoded protein product.

One of the approaches to large-scale sequencing is predicated upon the proposition that expressed sequences-that is, those accessible through isolation of mRNA-are of greatest initial interest. This"expressed

sequence tag" ("EST") approach has already yielded vast amounts of sequence data (see for example Adams et al., Science 252 : 1651 (1991) ; Williamson, Drug Discov. Today 4 : 115 (1999)). For nucleic acids sequenced by this approach, often the only biological information that is known a priori with any certainty is the likelihood of biologic expression itself. By virtue of the species and tissue from which the mRNA had originally been obtained, most such sequences are also annotated with the identity of the species and at least one tissue in which expression appears likely.

More recently, the pace of genomic sequencing has accelerated dramatically. When genomic DNA serves as the initial substrate for sequencing efforts, expression cannot be presumed ; often the only a priori biological information about the sequence includes the species and chromosome (and perhaps chromosomal map location) of origin.

With the ever-accelerating pace of sequence accumulation by directed, EST, and genomic sequencing approaches-and in particular, with the accumulation of sequence information from multiple genera, from multiple species within genera, and from multiple individuals within a species-there is an increasing need for methods that rapidly and effectively permit the functions of nucleic sequences to be elucidated. And as such functional information accumulates, there is a further need for methods of storing such functional information in meaningful and useful relationship to the sequence itself ; that is, there is an increasing need for means and apparatus for annotating raw sequence data with known or predicted functional information.

Although the increase in the pace of genomic sequencing is due in large part to technological changes in sequencing strategies and instrumentation, Service, Science 280 : 995 (1998) ; Pennisi, Science 283 : 1822-1823 (1999),

there is an important functional motivation as well.

While it was understood that the EST approach would rarely be able to yield sequence information about the noncoding portions of the genome, it now also appears the EST approach is capable of capturing only a fraction of a genome's actual expression complexity.

For example, when the C. elegans genome was fully sequenced, gene prediction algorithms identified over 19, 000 potential genes, of which only 7, 000 had been found by EST sequencing. C. elegans Sequencing Consortium, Science 282 : 2012 (1998). Analogously, the recently completed sequence of chromosome 2 of Arabidopsis predicts over 4000 genes, Lin et al., Nature, 402 : 761 (1999), of which only about 6% had previously been identified via EST sequencing efforts. Although the human genome has the greatest depth of EST coverage, it is still woefully short of surrendering all of its genes. One recent estimate suggests that the human genome contains more than 146, 000 genes, which would at this point leave greater than half of the genes undiscovered. It is now predicted that many genes, perhaps 20 to 50%, will only be found by genomic sequencing.

There is, therefore, a need for methods that permit the functional regions of genomic sequence-and most importantly, but not exclusively, regions that function to encode genes-to be identified.

Much of the coding sequence of the human genome is not homologous to known genes, making detection of open reading frames ("ORFs") and predictions of gene function difficult. Computational methods exist for predicting coding regions in eukaryotic genomes. Gene prediction programs such as GRAIL and GRAIL II, Uberbacher et al., Proc. Natl. Acad. Sci. USA 88 (24) : 11261-5 (1991) ; Xu et al., Genet. Eng. 16 : 241-53 (1994) ; Uberbacher et al., Methods Enzymol. 266 : 259-81 (1996) ; GENEFINDER, Solovyev et

al., Nucl. Acids. Res. 22 : 5156-63 (1994) ; Solovyev et al., Ismb 5 : 294-302 (1997) ; and GENESCAN, Burge et al., J. Mol.

Biol. 268 : 78-94 (1997), predict many putative genes without known homology or function. Such programs are known, however, to give high false positive rates. Burset et al., Genomics 34 : 353-367 (1996). Using a consensus obtained by a plurality of such programs is known to increase the reliability of calling exons from genomic sequence.

Ansari-Lari et al., Genome Res. 8 (1) : 29-40 (1998) Identification of functional genes from genomic data remains, however, an imperfect art. For example, in reporting the full sequence of human chromosome 21, the Chromosome 21 Mapping and Sequencing Consortium reports that prior bioinformatic estimates of human gene number may need to be revised substantially downwards. Nature 405 : 311-199 (2000) ; Reeves, Nature 405 : 283-284 (2000).

Thus, there is a need for methods and apparatus that permit the functions of the regions identified bioinformatically-and specifically, that permit the expression of regions predicted to encode protein-readily to be confirmed experimentally.

Recently, the development of nucleic acid microarrays has made possible the automated and highly parallel measurement of gene expression. Reviewed in Schena (ed.), DNA Microarrays : A Practical Approach (Practical Approach Series), Oxford University Press (1999) (ISBN : 0199637768) ; Nature Genet. 21 (1) (suppl) : 1-60 (1999) ; Schena (ed.), Microarray Biochip : Tools and Technology, Eaton Publishing Company/BioTechniques Books Division (2000) (ISBN : 1881299376).

It is common for microarrays to be derived from cDNA/EST libraries, either from those previously described in the literature, such as those from the I. M. A. G. E. consortium, Lennon et al., Genomics 33 (1) : 151-2 (1996), or from the construction of"problem specific"libraries

targeted at a particular biological question, R. S. Thomas et al., Cancer Res. (in press). Such microarrays by definition can measure expression only of those genes found in EST libraries, and thus have not been useful as probes for genes discovered solely by genomic sequencing.

The utility of using whole genome nucleic acid microarrays to answer certain biological questions has been demonstrated for the yeast Saccharomyces cerevisiae. De Risi et al., Science 278 : 680 (1997). The vast majority of yeast nuclear genes, approximately 95% however, are single exon genes, i. e., lack introns, Lopez et al., RNA 5 : 1135- 1137 (1999) ; Goffeau et al., Science 274 : 563-67 (1996), permitting coding regions more readily to be identified.

Whole genome nucleic acid microarrays have not generally been used to probe gene expression from more complex eukaryotic genomes, and in particular from those averaging more than one intron per gene.

Diseases of the breast are a significant cause of human morbidity and mortality. Increasingly, genetic factors are being found that contribute to predisposition, onset, and/or aggressiveness of most, if not all, of these diseases. Although mutations in single genes have been identified as causative for some diseases of the breast, for the most part these disorders are believed to have polygenic etiologies. There is a need for methods and apparatus that permit prediction, diagnosis and prognosis of diseases of the human breast, particularly those diseases with polygenic etiology.

Summary of the Invention The present invention solves these and other problems in the art by providing methods and apparatus for predicting, confirming, and displaying functional information derived from genomic sequence. The present

invention also provides apparatus for verifying the expression of putative genes identified within genomic sequence.

In particular, the invention provides novel genome-derived single exon nucleic acid microarrays useful for verifying the expression of putative genes identified within genomic sequence.

The present invention also provides compositions and kits for the ready production of nucleic acids identical in sequence to, or substantially identical in sequence to, probes on the genome-derived single exon microarrays of the present invention.

Accordingly, in a first aspect of the invention, there is provided a spatially-addressable set of single exon nucleic acid probes for measuring gene expression in a sample derived from human Breast, comprising a plurality of single exon nucleic acid probes according to any one of the nucleotide sequences set out in SEQ ID NOs : 1-5, 074 or a complementary sequence, or a portion of such a sequence.

By plurality is meant at least two, suitably at least 20, most suitably at least 100, preferably at least 1000 and, most preferably, upto 5000.

In one embodiment of the first aspect, each of said plurality of probes is separately and addressably amplifiable.

In an alternative embodiment, each of said plurality of probes is separately and addressably isolatable from said plurality.

In a preferred embodiment, each of said plurality of probes is amplifiable using at least one common primer.

Preferably, each of said plurality of probes is amplifiable using a first and a second common primer.

In yet another embodiment, said set of single exon nucleic acid probes comprises between 50-20, 000 probes, for example, 50-5000.

Suitably, said set of single exon nucleic acid probes comprises at least 50-1000 discrete single exon nucleic acid probes having a sequence as set out in any of SEQ ID NOS. : 1-10, 058 or a complimentary sequence, or a portion of such a sequence.

Preferably, the average length of the single exon nucleic acid probes is between 200 and 500 bp. It is preferred that the average length should be at least 200bp, suitably at least 250bp, most suitably at least 300bp, preferably at least 400bp and, most preferably, 500 bp.

In another embodiment, the single exon nucleic acid probes lack prokaryotic and bacteriophage vector sequence. It is preferred that at least 50%, suitably at least 60%, most suitably at least 70%, preferably at least 75%, more preferably at least 80, 85, 90, 95 or 99% of said single exon nucleic acid probes lack prokaryotic and bacteriophage vector sequence.

In another preferred embodiment, said single exon nucleic acid lack homopolymeric stretches of A or T. It is preferred that at least 50%, suitably at least 60%, most suitably at least 70%, preferably at least 75%, more preferably at least 80, 85, 90, 95 or 99% of said single exon nucleic acid probes lack homopolymeric stretches of A or T.

Preferably, a spatially-addressable set of single exon nucleic acid probes in accordance with the first aspect of the invention is is addressably disposed upon a substrate.

Suitable substrates include a filter membrane which may, preferably, be nitrocellulose or nylon. The nylon may preferably, be positively-charged. Other suitable substrate's include glass, amorphous silicon, crystalline silicon, and plastic. Further suitable materials include polymethylacrylic, polyethylene, polypropylene, polyacrylate, polymethylmethacrylate, polyvinylchloride,

polytetrafluoroethylene, polystyrene, polycarbonate, polyacetal, polysulfone, celluloseacetate, cellulosenitrate, nitrocellulose, and mixtures thereof.

In a second aspect of the invention, there is provided a microarray comprising a spatially addressable set of single exon nucleic acid probes in accordance with the first aspect of the invention.

In one embodiment, a genome-derived single-exon microarray is packaged together with such an ordered set of amplifiable probes corresponding to the probes, or one or more subsets of probes, thereon. In alternative embodiments, the ordered set of amplifiable probes is packaged separately from the genome-derived single exon microarray.

In another aspect, the invention provides genome- derived single exon nucleic acid probes useful for gene expression analysis, and particularly for gene expression analysis by microarray. In particular embodiments of this aspect, the present invention provides human single-exon probes that include specifically-hybridizable fragments of SEQ ID Nos. 5, 075-10, 058, wherein the fragment hybridizes at high stringency to an expressed human gene. In particular embodiments, the invention provides single exon probes comprising SEQ ID Nos. 1-5, 074.

Accordingly, in a third aspect of the invention, there is provided a single exon nucleic acid probe for measuring human gene expression in a sample derived from human Breast which is a nucleic acid molecule comprising a nucleotide sequence as set out in any of SEQ ID NOs. : 1- 5, 074 or a complementary sequence or a fragment thereof wherein said probe hybridizes at high stringency to a nucleic acid expressed in the human Breast.

In one embodiment, a single exon nucleic acid probe in accordance with the third aspect comprises a nucleotide sequence as set out in any of SEQ ID NOs. : 5, 075

-10, 058 or a complementary sequence or a fragment thereof.

In a fourth aspect of the invention, there is provided a single exon nucleic acid probe for measuring human gene expression in a sample derived from human Breast which is a nucleic acid molecule having a sequence encoding a peptide comprising a peptide sequence as set out in any of SEQ ID NOs. : 10, 059-15, 009 or a complementary sequence or a fragment thereof wherein said probe hybridizes at high stringency to a nucleic acid expressed in the human Breast.

Preferably, a single exon nucleic acid probe in accordance with the third or fourth aspects of the invention comprises between at least 15 and 50 contiguous nucleotides of said SEQ ID NO :. It is preferred that the single exon nucleic acid probe comprises at least 15, suitably at least 20, more suitably at least 25 or preferably at least 50 contiguous nucleotides of said SEQ ID NO :.

In another preferred embodiment,-a single exon nucleic acid probe in accordance with the third or fourth aspects of the invention is between 3kb and 25kb in length.

It is preferred that said probe is no more than 3kb, suitably no more than 5kb, more suitably no more than lob, preferably 15kb, more preferably 20kb or, most preferably, no more than 20kb in length.

Preferably, a single exon nucleic acid probe in accordance with either the fifth or sixth aspect of the invention is DNA, preferably single-stranded DNA, RNA or PNA.

In another embodiment of either the third or fourth aspect of the invention, a single exon nucleic acid probe is detectably labeled. Suitable detectable labels include a radionuclide, a fluorescent label or a first member of a specific binding pair. Suitable fluorescent labels include dyes such as cyanine dyes, preferably Cy3 and Cy5 although other suitable dyes will be known to those

skilled in the art.

In a particularly preferred embodiment, a single exon nucleic acid probe in accordance with either the third or fourth aspect of the invention lacks prokaryotic and bacteriophage vector sequence. In yet another embodiment, a single exon nucleic acid probe in accordance with either the third or fourth aspect of the invention lacks homopolymeric stretches of A or T.

In a fifth aspect of the invention, there is provided an amplifiable nucleic acid composition, comprising : the single exon nucleic acid probe in accordance with either of the third or fourth aspects of the invention ; and at least one nucleic acid primer ; wherein said at least one primer is sufficient to prime enzymatic amplification of said probe.

In an sixth aspect of the invention, there is provided a method of measuring gene expression in a sample derived from human Breast, comprising : contacting the single exon microarray in accordance with the second aspect of the invention, with a first collection of detectably labeled nucleic acids, said first collection of nucleic acids derived from mRNA of human Breast ; and then measuring the label detectably bound to each probe of said microarray.

In a seventh aspect of the invention, there is provided a method of identifying exons in a eukaryotic genome, comprising : algorithmically predicting at least one exon from genomic sequence of said eukaryote ; and then detecting specific hybridization of detectably labeled nucleic acids to a single exon probe, wherein said detectably labeled nucleic acids are derived from mRNA from the Breast of said eukaryote, said

probe is a single exon probe having a fragment identical in sequence to, or complementary in sequence to, said predicted exon, said probe is included within a single exon microarray in accordance with the first aspect of the invention, and said fragment is selectively hybridizable at high stringency.

In a eighth aspect of the invention, there is provided a method of assigning exons to a single gene, comprising : identifying a plurality of exons from genomic sequence in accordance with the seventh aspect of the invention ; and then measuring the expression of each of said exons in a plurality of tissues and/or cell types using hybridization to single exon microarrays having a probe with said exon, wherein a common pattern of expression of said exons in said plurality of tissues and/or cell types indicates that the exons should be assigned to a single gene.

In an ninth aspect of the invention, there is provided a nucleic acid sequence as set out in any of SEQ ID NOs : 1-10, 058 wherein said sequence encodes a peptide.

In a tenth aspect of the invention, there is provided a peptide encoded by a sequence comprising a sequence as set out in any of SEQ ID NOs : 5, 075-10, 058, or a complementary sequence or coding portion thereof.

In a preferred embodiment, a peptide may be encoded by a sequence comprising a sequence set out in any of SE ID NOS. : 1-5, 074.

In a further aspect, the invention provides peptides comprising an amino acid sequence translated from the DNA fragments, said amino acid sequences comprising SEQ ID NOS. : 10, 059-15, 009. Accordingly in a eleventh aspect of he invention

there is provided a peptide comprising a sequence as set out in any of SEQ ID NOs : 10, 059-15, 009, or fragment thereof.

In another aspect, the invention provides means for displaying annotated sequence, and in particular, for displaying sequence annotated according to the methods and apparatus of the present invention. Further, such display can be used as a preferred graphical user interface for electronic search, query, and analysis of such annotated sequence.

Detailed Description of the Invention Definitions As used herein, the term"microarray"and phrase "nucleic acid microarray"refer to a substrate-bound collection of plural nucleic acids, hybridization to each of the plurality of bound nucleic acids being separately detectable. The substrate can be solid or porous, planar or non-planar, unitary or distributed.

As so defined, the term"microarray"and phrase "nucleic acid microarray"include all the devices so called in Schena (ed.), DNA Microarrays : A Practical Approach (Practical Approach Series), Oxford University Press (1999) (ISBN : 0199637768) ; Nature Genet. 21 (1) (suppl) : 1-60 (1999) ; and Schena (ed.), Microarray Biochip : Tools and Technology, Eaton Publishing Company/BioTechniques Books Division (2000) (ISBN : 1881299376). As so defined, the term"microarray"and phrase"nucleic acid microarray" further include substrate-bound collections of plural nucleic acids in which the nucleic acids are distributably disposed on a plurality of beads, rather than on a unitary planar substrate, as is described, inter alia, in Brenner et al., Proc. Natl. Acad. Sci. USA 97 (4) : 166501670 (2000) ;

in such case, the term"microarray"and phrase"nucleic acid microarray"refer to the plurality of beads in aggregate.

As used herein with respect to a nucleic acid microarray, the term"probe"refers to the nucleic acid that is, or is intended to be, bound to the substrate ; in such context, the term"target"thus refers to nucleic acid intended to be bound thereto by Watson-Crick complementarity. As used herein with respect to solution phase hybridization, the term"probe"refers to the nucleic acid of known sequence that is detectably labeled.

As used herein, the expression"probe comprising SEQ ID NO.", and variants thereof, intends a nucleic acid probe, at least a portion of which probe has either (i) the sequence directly as given in the referenced SEQ ID NO., or (ii) a sequence complementary to the sequence as given in the referenced SEQ ID NO., the choice as between sequence directly as given and complement thereof dictated by the requirement that the probe hybridize to mRNA.

As used herein, the term"open reading frame"and the equivalent acronym"ORF"refer to that portion of an exon that can be translated in its entirety into a sequence of contiguous amino acids i. e. a nucleic acid sequence that, in at least one reading frame, does not possess stop codons ; the term does not require that the ORF encode the entirety of a natural protein.

As used herein, the term"amplicon"refers to a PCR product amplified from human genomic DNA, containing the predicted exon.

As used herein the term"exon"refers to the consensus prediction of the various exon and gene predicting algorithms i. e. a nucleic acid sequence bioinformatically predicted to encode a portion of a natural protein.

As used herein, the term"peptide"refers to a

sequence of amino acids. The sequences referred to as PEPTIDE SEQ ID NOS. : are the predicted peptide sequences that would be translated from one of the exons, or a portion thereof set out in exon SEQ ID NOS. :. The codons encoding the peptide are wholly contained within the exon.

As used herein, a"portions"of a defined nucleotide sequence or sequences can be and, preferably, are fragments unique to that sequence or to one or a combination of those sequences. A fragment unique to a nucleic acid molecule is one that is a signature for the larger nucleic acid molecule.

As used herein, the phrase"expression of a probe"and its linguistic variants means that the ORF present within the probe, or its complement, is present within a target mRNA.

As used herein,"stringent conditions"refers to parameters well known to those skilled in the art. When a nucleic acid molecule is said to be hybridisable to another of a given sequence under"stringent conditions"it is meant that it is homologous to the given sequence.

As used herein, the phrase"specific binding pair"intends a pair of molecules that bind to one another with high specificity. Binding pairs are said to exhibit specific binding when they exhibit avidity of at least 107, preferably at least 108, more preferably at least 109 liters/mole. Nonlimiting examples of specific binding pairs are : antibody and antigen ; biotin and avidin ; and biotin and streptavidin.

As used herein with respect to the visual display of annotated genomic sequence, the term"rectangle"means any geometric shape that has at least a first and a second border, wherein the first and second borders each are capable of mapping uniquely to a point of another visual object of the display.

As used herein, a"Mondrian"means a visual

display in which a single genomic sequence is annotated with predicted and experimentally confirmed functional information.

Brief Description of the Drawings The present invention is further illustrated with reference to the following non-limiting figures and examples in which : FIG. 1 illustrates a process for predicting functional regions from genomic sequence, confirming the functional activity of such regions experimentally, and associating and displaying the data so obtained in meaningful and useful relationship to the original sequence data ; FIG. 2 further elaborates that portion of the process schematized in FIG. 1 for predicting functional regions from genomic sequence ; FIG. 3 illustrates a Mondrian visual display ; FIG. 4 presents a Mondrian showing a hypothetical annotated genomic sequence ; FIG. 5 is a histogram showing the distribution of ORF length and PCR products as obtained, with ORF length shown in black and PCR product length shown in dotted lines ; FIG. 6 is a histogram showing the distribution, among exons predicted according to the methods described, of expression as measured using simultaneous two color hybridization to a genome-derived single exon microarray.

The graph shows the number of sequence-verified products that were either not expressed ("0"), expressed in one or more but not all tested tissues ("1"-"9"), or expressed in all tissues tested ("10") ; FIG. 7 is a pictorial representation of the

expression of verified sequences that showed expression with signal intensity greater than 3 in at least one tissue, with : FIG. 7A showing the expression as measured by microarray hybridization in each of the 10 measured tissues, and the expression as measured"bioinformatically" by query of EST, NR and SwissProt databases ; with FIG. 7B showing the legend for display of physical expression (ratio) in FIG. 7A ; and with FIG. 7C showing the legend for scoring EST hits as depicted in FIG. 7A ; FIG. 8 shows a comparison of normalized CY3 signal intensity for arrayed sequences that were identical to sequences in existing EST, NR and SwissProt databases or that were dissimilar (unknown), where black denotes the signal intensity for all sequence-verified products with a BLAST Expect ("E") value of greater than le-30 (1 x 10-30) ("unknown") and a dotted line denotes sequence-verified spots with a BLAST expect ("E") value of less than le-30 (1 X 10-3) ("known") ; FIG. 9 presents a Mondrian of BAC AC008172 (bases 25, 000 to 130, 000), containing the carbamyl phosphate synthetase gene (AF154830. 1) ; and FIG. 10 is a Mondrian of BAC A049839.

Methods and Apparatus for Predicting, Confirming, Annotating, and Displaying Functional Regions From Genomic Sequence Data FIG. 1 is a flow chart illustrating in broad outline a process for predicting functional regions from genomic sequence, confirming and characterizing the functional activity of such regions experimentally, and then associating and displaying the information so obtained in meaningful and useful relationship to the original sequence data.

The initial input into process 10 of the present invention is drawn from one or more databases 100 containing genomic sequence data. Because genomic sequence is usually obtained from subgenomic fragments, the sequence data typically will be stored in a series of records corresponding to these subgenomic sequenced fragments.

Some fragments will have been catenated to form larger contiguous sequences ("contigs") ; others will not. A finite percentage of sequence data in the database will typically be erroneous, consisting inter alia of vector sequence, sequence created from aberrant cloning events, sequence of artificial polylinkers, and sequence that was erroneously read.

Each sequence record in database 100 will minimally contain as annotation a unique sequence identifier (accession number), and will typically be annotated further to identify the date of accession, species of origin, and depositor., Because database 100 can contain nongenomic sequence, each sequence will typically be annotated further to permit query for genomic sequence.

Chromosomal origin, optionally with map location, can also be present. Data can be, and over time increasingly will be, further annotated with additional information, in part through use of the present invention, as described below.

Annotation can be present within the data records, in information external to database 100 and linked to the records thereto, or through a combination of the two.

Databases useful as genomic sequence database 100 in the present invention include GenBank, and particularly include several divisions thereof, including the htgs (draft), NT (nucleotide, command line), and NR (nonredundant) divisions. GenBank is produced by the National Institutes of Health and is maintained by the National Center for Biotechnology Information (NCBI).

Databases of genomic sequence from species other than

human, such as mouse, rat, Arabidopsis, C. elegans, C. brigsii, Drosophila, zebra fish, and other higher eukaryotic organisms will also prove useful as genomic sequence database 100.

Genomic sequence obtained by query of genomic sequence database 100 is then input into one or more processes 200 for identification of regions therein that are predicted to have a biological function as specified by the user. Such functions include, but are not limited to, encoding protein, regulating transcription, regulating message transport after transcription into mRNA, regulating message splicing after transcription into mRNA, of regulating message degradation after transcription into mRNA, and the like. Other functions include directing somatic recombination events, contributing to chromosomal stability or movement, contributing to allelic exclusion or X chromosome inactivation, and the like.

The particular genomic sequence to be input into process 200 will depend upon the function for which relevant sequence is to be identified as well as upon the approach chosen for such identification. Process step 200 can be iterated to identify different functions within a given genomic region. In such case, the input often will be different for the several iterations.

Sequences predicted to have the requisite function by process 200 are then input into process 300, where a subset of the input sequences suitable for experimental confirmation is identified. Experimental confirmation can involve physical and/or bioinformatic assay. Where the subsequent experimental assay is bioinformatic, rather than physical, there are fewer constraints on the sequences that can be tested, and in this latter case therefore process 300 can output the entirety of the input sequence.

The subset of sequences output from process 300

is then used in process 400 for experimental verification and characterization of the function predicted in process 200, which experimental verification can, and often will, include both physical and bioinformatic assay.

Process 500 annotates the sequence data with the functional information obtained in the physical and/or bioinformatic assays of process 400. Such annotation can be done using any technique that usefully relates the functional information to the sequence, as, for example, by incorporating the functional data into the sequence data record itself, by linking records in a hierarchical or relational database, by linking to external databases, by a combination thereof, or by other means well known within the database arts. The data can even be submitted for incorporation into databases maintained by others, such as GenBank, which is maintained by NCBI.

As further noted in FIG. 1, additional annotation can be input into process 500 from external sources 600.

The annotated data is then displayed in process 800, either before, concomitantly with, or after optional storage 700 on nontransient media, such as magnetic disk, optical disc, magnetooptical disk, flash memory, or the like.

FIG. 1 shows that the experimental data output from process 400 can be used in each preceding step of process 10 : e. g., facilitating identification of functional sequences in process 200, facilitating identification of an experimentally suitable subset thereof in process 300, and facilitating creation of physical and/or informational substrates for, and performance of subsequent assay, of functional sequences in process 400.

Information from each step can be passed directly to the succeeding process, or stored in permanent or interim form prior to passage to the succeeding process.

Often, data will be stored after each, or at least a

plurality, of such process steps. Any or all process steps can be automated.

FIG. 2 further elaborates the prediction of functional sequence within genomic sequence according to process 200.

Genomic sequence database 100 is first queried 20 for genomic sequence.

The sequence required to be returned by query 20 will depend, in the first instance, upon the function to be identified.

For example, genomic sequences that function to encode protein can be identified inter alia using gene prediction approaches, comparative sequence analysis approaches, or combinations of the two. In gene prediction analysis, sequence from one genome is input into process 200 where at least one, preferably a plurality, of algorithmic methods are applied to identify putative coding regions. In comparative sequence analysis, by contrast, corresponding, e. g., syntenic, sequence from a plurality of sources, typically a plurality of species, is input into process 200, where at least one, possibly a plurality, of algorithmic methods are applied to compare the sequences and identify regions of least variability.

The exact content of query 20 will also depend upon the database queried. For example, if the database contains both genomic and nongenomic sequence, perhaps derived from multiple species, and the function to be determined is protein coding regions in human genomic sequence, the query will accordingly require that the sequence returned be genomic and derived from humans.

Query 20 can also incorporate criteria that compel return of sequence that meets operative requirements of the subsequent analytical method. Alternatively, or in addition, such operative criteria can be enforced in subsequent preprocess step 24.

For example, if the function sought to be identified is protein coding,. query 20 can incorporate criteria that return from genomic sequence database 100 only those sequences present within contigs sufficiently long as to have obviated substantial fragmentation of any given exon among a plurality of separate sequence fragments.

Such criteria can, for example, consist of a required minimal individual genomic sequence fragment length, such as 10 kb, more typically 20 kb, 30 kb, 40kb, and preferably 50 kb or more, as well as an optional further or alternative requirement that sequence from any given clone, such as a bacterial artificial chromosome ("BAC"), be presented in no more than a finite maximal number of fragments, such as no more than 20 separate pieces, more typically no more than 15 fragments, even more typically no more than about 10-12 fragments.

Results using the present invention have shown that genomic sequence from bacterial artificial chromosomes (BACs) is sufficient for gene prediction analysis according to the present invention if the sequence is at least 50 kb in length, and if additionally the sequence from any given BAC is presented in fewer than 15, and preferably fewer than 10, fragments. Accordingly, query 20 can incorporate a requirement that data accessioned from BAC sequencing be in fewer than 15, preferably fewer than 10, fragments.

An additional criterion that can be incorporated into the query can be the date, or range of dates, of sequence accession. Although the process has been described above as if genomic sequence database 100 were static, it is of course understood that the genomic sequence databases need not be static, and indeed are typically updated on a frequent, even hourly, basis. Thus, as further described in Examples 1 and 2, infra, it is possible to query the database for newly added sequence,

either newly added after an absolute date, or newly added relative to a prior analysis performed using the methods and apparatus of the present invention. In this way, the process herein described can incorporate a dynamic, temporal component.

One utility of such temporal limitation is to identify, from newly accessioned genomic sequence, the presence of novel genes, particularly those not previously identified by EST sequencing (or other sequencing efforts that are similarly based upon gene expression). As further described in Example 1, such an approach has shown that newly accessioned human genomic sequence, when analyzed for sequences that function to encode protein, readily identifies genes that are novel over those in existing EST and other expression databases. This makes the methods of the present invention extremely powerful gene discovery tools. And as would be appreciated, such gene discovery can be performed using genomic sequence from species other than human.

If query 20 incorporates multiple criteria, such as above-described, the multiple criteria can be performed as a series of separate queries or as a single query, depending in part upon the query language, the complexity of the query, and other considerations well known in the database arts.

If query 20 returns no genomic sequence meeting the query criteria, the negative result can be reported by process 22, and process 200 (and indeed, entire process 10) ended 23, as shown. Alternatively, or in addition to report and termination of the initial inquiry, a new query 20 can be generated that takes into account the initial negative result.

When query 20 returns sequence meeting the query criteria, the returned sequence is then passed to optional preprocessing 24, suitable and specific for the desired

analytical approach and the particular analytical methods thereof to be used in process 25.

Preprocessing 24 can include processes suitable for many approaches and methods thereof, as well as processes specifically suited for the intended subsequent analysis.

Preprocessing 24 suitable for most approaches and methods will include elimination of sequence irrelevant to, or that would interfere with, the subsequent analysis.

Such sequence includes repetitive sequence, such as Alu repeats and LINE elements, vector sequence, artificial sequence, such as artificial polylinkers, and the like.

Such removal can readily be performed by identification and subsequent masking of the undesired sequence.

Identification can be effected by comparing the genomic sequence returned by query 20 with public or private databases containing known repetitive sequence, vector sequence, artificial sequence, and other artifactual sequence. Such comparison can readily be done using programs well known in the art, such as CROSS MATCH, or by proprietary sequence comparison programs the engineering of which is well within the skill in the art.

Alternatively, or in addition, undesirable, including artifactual, sequence can be identified algorithmically without comparison to external databases and thereafter removed. For example, synthetic polylinker sequence can be identified by an algorithm that identifies a significantly higher than average density of known restriction sites. As another example, vector sequence can be identified by algorithms that identify nucleotide or codon usage at variance with that of the bulk of the genomic sequence.

Once identified, undesired sequence can be removed. Removal can usefully be done by masking the undesired sequence as, for example, by converting the

specific nucleotide references to one that is unrecognized by the subsequent bioinformatic algorithms, such as"X".

Alternatively, but at present less preferred, the undesired sequence can be excised from the returned genomic sequence, leaving gaps.

Preprocessing 24 can further include selection from among duplicative sequences of that one sequence of highest quality. Higher quality can be measured as a lower percentage of, fewest number of, or least densely clustered occurrence of ambiguous nucleotides, defined as those nucleotides that are identified in the genomic sequence using symbols indicating ambiguity. Higher quality can also or alternatively be valued by presence in the longest contig.

Preprocessing 24 can, and often will, also include formatting of the data as specifically appropriate for passage to the analytical algorithms of process 25.

Such formatting can and typically will include, inter alia, addition of a unique sequence identifier, either derived from the original accession number in genomic sequence database 100, or newly applied, and can further include additional annotation. Formatting can include conversion from one to another sequence listing standard, such as conversion to or from FASTA or the like, depending upon the input expected by the subsequent process.

Preprocessing, which can be optional depending upon the function desired to be identified and the informational requirements of the methods for effecting such identification, is followed by sequence processing 25, where sequences with the desired function are identified within the genomic sequence.

As mentioned above, such functions can include, but are not limited to, encoding protein, regulating transcription, regulating message transport after transcription into mRNA, regulating message splicing after

transcription, of regulating message degradation, and the like. Other functions include directing somatic recombination events, contributing to chromosomal stability or movement, contributing to allelic exclusion or X chromosome inactivation, or the like.

The methods of the present invention are particularly useful for gene discovery, that is, for identifying, from genomic sequence, regions that function to encode genes, and in a particularly useful embodiment, for identifying regions that function to encode genes not hitherto identified by expression-based or directed cloning and sequencing. In conjunction with verification using the novel single exon microarrays of the present invention, as further described below, the methods herein described become powerful gene discovery tools.

Accordingly, in a preferred embodiment of the present invention, process 25 is used to identify putative coding regions. Two preferred approaches in process 25 for identifying sequence that encodes putative genes are gene prediction and comparative sequence analysis.

Gene prediction can be performed using any of a number of algorithmic methods, embodied in one or more software programs, that identify open reading frames (ORFs) using a variety of heuristics, such as GRAIL, DICTION, and GENEFINDER. Comparative sequence analysis similarly can be performed using any of a variety of known programs that identify regions with lower sequence variability.

As further described in Example 1, below, gene finding software programs yield a range of results. For the newly accessioned human genomic sequence input in Example 1, for example, GRAIL identified the greatest percentage of genomic sequence as putative coding region, 2% of the data analyzed ; GENEFINDER was second, calling 1% ; and DICTION yielded the least putative coding region, with 0. 8% of genomic sequence called as coding region.

Increased reliability can be obtained when consensus is required among several such methods. Although discussed herein particularly with respect to exon calling, consensus among methods will in general increase reliability of predicting other functions as well.

Thus, as indicated by query 26, sequence processing 25, optionally with preprocessing 24, can be repeated with a different method, with consensus among such iterations determined and reported in process 27.

Process 27 compares the several outputs for a given input genomic sequence and identifies consensus among the separately reported results. The consensus itself, as well as the sequence meeting that consensus, is then stored in process 29a, displayed in process 29b, and/or output to process 300 for subsequent identification of a subset thereof suitable for assay.

Multiple levels of consensus can be calculated and reported by process 27. For example, as further described in Example 1, infra, process 27 can report consensus as between all specific pairs of methods of gene prediction, as consensus among any one or more of the pairs of methods of gene prediction, or as among all of the gene prediction algorithms used. Thus, in Example 1, process 27 reported that GRAIL and GENEFINDER programs agreed on 0. 7% of genomic sequence, that GRAIL and DICTION agreed on 0. 5% of genomic sequence, and that the three programs together agreed on 0. 25% of the data analyzed. Put another way, 0. 25% of the genomic sequence was identified by all three of the programs as containing putative coding region.

Furthermore, consensus can be required among different approaches to identifying a chosen function.

For example, if the function desired to be identified is coding of protein sequence, and a first used approach to exon calling is gene prediction, the process can be repeated on the same input sequence, or subset

thereof, with another approach, such as comparative sequence analysis. In such a case, where comparative sequence analysis follows gene prediction, the comparison can be performed not only on genomic nucleic acid sequence, but additionally or alternatively can be performed on the predicted amino acid sequence translated from the ORFs prior identified by the gene prediction approach.

Although shown as an iterative process, the multiple analyses required to achieve consensus can be done in series, in parallel, or some combination thereof.

Predicted functional sequence, optionally representing a consensus among a plurality of methods and approaches for determination thereof, is passed to process 300 for identification of a subset thereof for functional assay.

In the preferred embodiment of the methods of the present invention, wherein the function sought to be identified is protein coding, process 300 is used to identify a subset thereof suitable for experimental verification by physical and/or bioinformatic approaches.

For example, putative ORFs identified in process 200 can be classified, or binned, bioinformatically into putative genes. This binning can be based inter alia upon consideration of the average number of exons/gene in the species chosen for analysis, upon density of exons that have been called on the genomic sequence, and other empirical rules. Thereafter, one or more among the gene- specific ORFs can be chosen for subsequent use in gene expression assay.

Where such subsequent gene expression assay uses amplified nucleic acid, considerations such as desired amplicon length, primer synthesis requirements, putative exon length, sequence GC content, existence of possible secondary structure, and the like can be used to identify and select those ORFs that appear most likely successfully

to amplify. Where subsequent gene expression assay relies upon nucleic acid hybridization, whether or not using amplified product, further considerations involving hybridization stringency can be applied to identify that subset of sequences that will most readily permit sequence- specific discrimination at a chosen hybridization and wash stringency. One particular such consideration is avoidance of putative exons that span repetitive sequence ; such sequence can hybridize spuriously to nonspecific message, reducing specific signal in the hybridization.

For bioinformatic assay, there are fewer constraints on the sequences that can be tested experimentally, and in this latter case therefore process 300 can output the entirety of the input sequence.

The subset of sequences identified by process 300 as suitable for use in assay is then used in process 400 to create the physical and/or informational substrate for experimental verification of the predictions made in process 200, and thereafter to assay those substrates.

As mentioned, the methods of the present invention are particularly useful for identifying potential coding regions within genomic sequence. In a preferred embodiment of process 400, therefore, the expression of the sequences predicted to encode protein is verified. The combination of the predictive and experimental methods provides a powerful gene discovery engine.

Thus, in another aspect, the present invention provides methods and apparatus for verifying the expression of putative genes identified within genomic sequence. In particular, the invention provides a novel method of verifying gene expression in which expression of predicted ORFs is measured and confirmed using a novel type of nucleic acid microarray, the genome-derived single exon nucleic acid microarrays of the present invention.

Putative ORFs as predicted by a consensus of gene

calling, particularly gene prediction, algorithms in process 200, and as further identified as suitable by process 300, are amplified from genomic DNA using the polymerase chain reaction (PCR). Although PCR is conveniently used, other amplification approaches can also be used.

Amplification schemes can be designed to capture the entirety of each predicted ORF in an amplicon with minimal additional (that is, intronic or intergenic) sequence. Because ORFs predicted from human genomic sequence using the methods of the present invention differ in length, such an approach results in amplicons of varying length.

However, most predicted ORFs are shorter than 500 bp in length, and although amplicons of at least about 100 or 200 base pairs can be immobilized as probes on nucleic acid microarrays, early experimental results using the methods of the present invention have suggested that longer amplicons, at least about 400 or 500 base pairs, are more effective. Furthermore, certain advantages derive from application to the microarray of amplicons of defined size.

Therefore, amplification schemes can alternatively, and preferably, be designed to amplify regions of defined size, preferably at least about 300, 400 or 500 bp, centered about each predicted ORF. Such an approach results in a population of amplicons of limited size diversity, but that typically contain intronic and/or intergenic nucleic acid in addition to putative ORF.

Conversely, somewhat fewer than 10% of ORFs predicted from human genomic sequence according to the methods of the present invention exceed 500 bp in length.

Portions of such extended ORFs, preferably at least about 300, 400 or 500 bp in length, can be amplified. However, it has been discovered that the percentage success at amplifying pieces of such ORFs is low, and that such

putative exons are more effectively amplified when larger fragments, at least about 1000 or 1500 bp, and even as large as 2000 bp are amplified.

The putative ORFs selected in process 300 are thus input into one or more primer design programs, such as PRIMER3 (available online for use at http ://www-genome. wi. mit. edu/cgi-bin/primer/), with a goal of amplifying at least about 500 base pairs of genomic sequence centered within or about ORFs predicted to be no more than about 500 bp, or at least about 1000-1500 bp of genomic sequence for ORFs predicted to exceed 500 bp in length, and the primers synthesized by standard techniques.

Primers with the requisite sequences can be purchased commercially or synthesized by standard techniques.

Conveniently, a first predetermined sequence can be added commonly to the ORF-specific 5'primer and a second, typically different, predetermined sequence commonly added to each 3'ORF-unique primer. This serves to immortalize the amplicon, that is, serves to permit further amplification of any amplicon using a single set of primers complementary respectively to the common 5'and common 3'sequence elements. The presence of these "universal"priming sequences further facilitates later sequence verification, providing a sequence common to all amplicons at which to prime sequencing reactions. The common 5'and 3'sequences further serve to add a cloning site should any of the ORFs warrant further study.

Such predetermined sequence is usefully at least about 10, 12 or 15 nt in length, and usually does not exceed about 25 nt in length. The"universal"priming sequences used in the examples presented infra were each 16 nt long.

The genomic DNA to be used as substrate for amplification will come from the eukaryotic species from which the genomic sequence data had originally been

obtained, or a closely related species, and can conveniently be prepared by well known techniques from somatic or germline tissue or cultured cells of the organism. See, e. g., Short Protocols in Molecular Biology : A Compendium of Methods from Current Protocols in Molecular Biology, Ausubel et al. (eds.), 4th edition (April 1999), John Wiley & Sons (ISBN : 047132938X) and Maniatis et al., Molecular Cloning : A Laboratory Manual, 2nd edition (December 1989), Cold Spring Harbor Laboratory Press (ISBN : 0879693096). Many such prepared genomic DNAs are available commercially, with the human genomic DNAs additionally having certification of donor informed consent.

Although the intronic and intergenic material flanking putative coding regions in the amplicons could potentially interfere with hybridizations during microarray experiments, we have found, surprisingly, that differential expression ratios are not significantly affected. Rather, the predominant effect of exon size is to alter the absolute signal intensity, rather than its ratio. Equally surprising, the art had suggested that single exon probes would not provide sufficient signal intensity for high stringency hybridization analyses ; we find that such probes not only provide adequate signal, but have substantial advantages, as herein described.

After partial purification, as by size exclusion spin column, with or without confirmation as to amplicon quality as by gel electrophoresis, each amplicon (single exon probe) is disposed in an array upon a support substrate.

Methods for creating microarrays by deposition and fixation of nucleic acids onto support substrates are well known in the art (Reviewed by Schena et al., see above).

Typically, the support substrate will be glass,

although other materials, such as amorphous or crystalline silicon or plastics. Such plastics include polymethylacrylic, polyethylene, polypropylene, polyacrylate, polymethylmethacrylate, polyvinylchloride, polytetrafluoroethylene, polystyrene, polycarbonate, polyacetal, polysulfone, celluloseacetate, cellulosenitrate, nitrocellulose, or mixtures thereof, can also be used. Typically, the support will be rectangular, although other shapes, particularly circular disks and even spheres, present certain advantages. Particularly advantageous alternatives to glass slides as support substrates for array of nucleic acids are optical discs, as described in WO 98/12559.

The amplified nucleic acids can be attached covalently to a surface of the support substrate or, more typically, applied to a derivatized surface in a chaotropic agent that facilitates denaturation and adherence by presumed noncovalent interactions, or some combination thereof.

Robotic spotting devices useful for arraying nucleic acids on support substrates can be constructed using public domain specifications (The MGuide, version 2. 0, http ://cmgm. stanford. edu/pbrown/mguide/index. html), or can conveniently be purchased from commercial sources (MicroArray GenII Spotter and MicroArray GenIII Spotter, Molecular Dynamics, Inc., Sunnyvale, CA). Spotting can also be effected by printing methods, including those using ink jet technology.

As is well known in the art, microarrays typically also contain immobilized control nucleic acids.

For controls useful in providing measurements of background signal for the genome-derived single exon microarrays of the present invention, a plurality of E. coli genes can readily be used. As further described in Example 1, 16 or 32 E. coli genes suffice to provide a robust measure of

background noise in such microarrays.

As is well known in the art, the amplified product disposed in arrays on a support substrate to create a nucleic acid microarray can consist entirely of natural nucleotides linked by phosphodiester bonds, or alternatively can include either nonnative nucleotides, alternative internucleotide linkages, or both, so long as complementary binding can be obtained in the hybridization.

If enzymatic amplification is used to produce the immobilized probes, the amplifying enzyme will impose certain further constraints upon the types of nucleic acid analogs that can be generated.

Although particularly described herein as using high density microarrays constructed on planar substrates, the methods of the present invention for confirming the expression of ORFs predicted from genomic sequence can use any of the known types of microarrays, as herein defined, including lower density planar arrays, and microarrays on nonplanar, nonunitary, distributed substrates.

For example, gene expression can be confirmed using hybridization to lower density arrays, such as those constructed on membranes, such as nitrocellulose, nylon, and positively-charged derivatized nylon membranes.

Further, gene expression can also be confirmed using nonplanar, bead-based microarrays such as are described in Brenner et al., Proc. Natl. Acad. Sci. USA 97 (4) : 166501670 (2000) ; U. S. Patent No. 6, 057, 107 ; and U. S. Patent No.

5, 736, 330. In theory, a packed collection of such beads provides in aggregate a higher density of nucleic acid ~ probe than can be achieved with spotting or lithography techniques on a single planar substrate.

Planar microarrays on solid substrates, however, provide certain useful advantages, including high throughput and compatibility with existing readers. For example, each standard microscope slide can include at

least 1000, typically at least 2000, preferably 5000 and upto 10, 000-50, 000 or more nucleic acid probes of discrete sequence. The number of sequences deposited will depend on their required application.

Each putative gene can be represented in the array by a single predicted ORF. Alternatively, genes can be represented by more than one predicted ORF. For purposes of measuring differential splicing, more than one predicted ORF will be provided for a putative gene. And as is well known in the art, each probe of defined sequence, representing a single predicted ORF, can be deposited in a plurality of locations on a single microarray to provide redundancy of signal.

The genome-derived single exon microarrays described above differ in several fundamental and advantageous ways from microarrays presently used in the gene expression art, including (1) those created by deposition of mRNA-derived nucleic acids, (2) those created by in situ synthesis of oligonucleotide probes, and (3) those constructed from yeast genomic DNA.

Most nucleic acid microarrays that are in use for study of eukaryotic gene expression have as immobilized probes nucleic acids that are derived-either directly or indirectly-from expressed message. As discussed above, it is common, for example, for such microarrays to be derived from cDNA/EST libraries, either from those previously described in the literature, see Lennon et al., or from the de novo construction of"problem specific" libraries targeted at a particular biological question, R. S. Thomas et al., Cancer Res. (in press). Such microarrays are herein collectively denominated"EST microarrays".

Such EST microarrays by definition can measure expression only of those genes found in EST libraries, shown herein to represent only a fraction of expressed

genes. Furthermore, such libraries-and thus microarrays based thereupon-are biased by the tissue or cell type of message origin, by the expression levels of the respective genes within the tissues, and by the ability of the message successfully to have been reverse-transcribed and cloned.

Thus, as further discussed in Example 1, the methods of the present invention enable sequences that do not appear in EST or other expression databases to be determined-subsequently arrayed for expression measurements could not, therefore, have been represented as probes on an EST microarray. And as further demonstrated in the examples, infra, the remaining population of genes identified from genomic sequence by the methods of the present invention-that is, the one third of sequences that had previously been accessioned in EST or other expression databases-are biased toward genes with higher expression levels.

Representation of a message in an EST and/or cDNA library depends upon the successful reverse transcription, optionally but typically with subsequent successful cloning, of the message. This introduces substantial bias into the population of probes available for arraying in EST microarrays.

In contrast, neither reverse transcription nor cloning is required to produce the probes arrayed on the genome-derived single exon microarrays of the present invention. And although the ultimate deposition of a probe on the genome-derived single exon microarray of the present invention depends upon a successful amplification from genomic material, a priori knowledge of the sequence of the desired amplicon affords greater opportunity to recover any given probe sequence recalcitrant to amplification than is afforded by the requirement for successful reverse transcription and cloning of unknown message in EST approaches.

Thus, the genome-derived single exon microarrays of the present invention present a far greater diversity of probes for measuring gene expression, with far less bias, than do EST microarrays presently used in the art.

As a further consequence of their ultimate origin from expressed message, the probes in EST microarrays often contain poly-A (or complementary poly-T) stretches derived from the poly-A tail of mature mRNA. These homopolymeric stretches contribute to cross-hybridization, that is, to a spurious signal occasioned by hybridization to the homopolymeric tail of a labeled cDNA that lacks sequence homology to the gene-specific portion of the probe.

In contrast, the probes arrayed in the genome- derived single exon microarrays of the present invention lack homopolymeric stretches derived from message polyadenylation, and thus can provide more specific signal.

Typically, at least about 50, 60 or 75% of the probes on the genome-derived single exon microarrays of the present invention lack homopolymeric regions consisting of A or T, where a homopolymeric region is defined for purposes herein as stretches of 25 or more, typically 30 or more, identical nucleotides.

A further distinction, which also affects the specificity of hybridization, is occasioned by the typical derivation of EST microarray probes from cloned material.

Because much of the probe material disposed as probes on EST microarrays is excised or amplified from plasmid, phage, or phagemid vectors, EST microarrays typically include a fair amount of vector sequence, more so when the. probes are amplified, rather than excised, from the vector.

In contrast, the vast majority of probes in the genome-derived single exon microarrays of the present invention contain no prokaryotic or bacteriophage vector sequence, having been amplified directly or indirectly from genomic DNA. Typically, therefore, at least about 50, 60,

70 or 80% or more of individual exon-including probes disposed on a genome-derived single exon microarray of the present invention lack vector sequence, and particularly lack sequences drawn from plasmids and bacteriophage.

Preferably, at least about 85, 90 or more than 90% of exon- including probes in the genome-derived single exon microarray of the present invention lack vector sequence.

With attention to removal of vector sequences through preprocessing 24, percentages of vector-free exon-including probes can be as high as 95-99%. The substantial absence of vector sequence from the genome-derived single exon microarrays of the present invention results in greater specificity during hybridization, since spurious cross- hybridization to a probe vector sequence is reduced.

As a further consequence of excision or amplification of probes from vectors in construction of EST microarrays, the probes arrayed thereon often contain artificial sequence, derived from vector polylinker multiple cloning sites, at both 5'and 3'ends. The probes disposed upon the genome-derived single exon microarrays need have no such artificial sequence appended thereto.

As mentioned above, however, the ORF-specific primers used to amplify putative ORFs can include artificial sequences, typically 5'to the ORF-specific primer sequence, useful for"universal" (that is, independent of ORF sequence) priming of subsequent amplification or sequencing reactions. When such "universal"5'and/or 3 priming sequences are appended to the amplification primers, the probes disposed upon the genome-derived single exon microarray will include artificial sequence similar to that found in EST microarrays. However, the genome-derived single exon microarray of the present invention can be made without such sequences, and if so constructed, presents an even smaller amount of nonspecific sequence that would

contribute to nonspecific hybridization.

Yet another consequence of typical use of cloned material as probes in EST microarrays is that such microarrays contain probes that result from cloning artifacts, such as chimeric molecules containing coding region of two separate genes. Derived from genomic material, typically not thereafter cloned, the probes of the genome-derived single exon microarrays of the present invention lack such cloning artifacts, and thus provide greater specificity of signal in gene expression measurements.

A further consequence of the cloned origin of probes on many EST microarrays is that the individual probes often have disparate sizes, which can cause the optimal hybridization stringency to vary among probes on a single microarray. In contrast, as discussed above, the probes arrayed on the genome-derived single exon microarrays of the present invention can readily be designed to have a narrow distribution in sizes, with the range of probe sizes no greater than about 10% of the average size, typically no greater than about 5% of the average probe size.

Because of their origin from fully-or partially- spliced message, probes disposed upon EST arrays will often include multiple exons. The percentage of such exon- spanning probes in an EST microarray can be calculated, on average, based upon the predicted number of exons/gene for the given species and the average length of the immobilized probes. For human genes, the near-complete sequence of human chromosome 22, Dunham et al., Nature 402 (6761) : 489-95 (1999), predicts that human genes average 5. 5 exons/gene.

Even with probes of 200-500 bp, the vast majority of human EST microarray probes include more than one exon.

In contrast, by virtue of their origin from algorithmically identified ORFs in genomic sequence, the

probes in the genome-derived single exon microarrays of the present invention can consist of individual exons. Thus, in contrast to EST microarrays, at least about 50, 60, 70, 75, 80, 85, 95 or 99% of probes deposited in the genome- derived microarray of the present invention consist of, or include, no more than one predicted ORF.

This provides the ability, not readily achieved using EST microarrays, to use the genome-derived single exon microarrays of the present invention to measure tissue-specific expression of individual exons, which in turn allows differential splicing events to be detected and characterized, and in particular, allows the correlation of differential splicing to tissue-specific expression patterns.

Furthermore, the exons that are represented in EST microarrays are often biased toward the 3'or 5'end of their respective genes, since sequencing strategies used for EST identification are so biased. In contrast, no such 3'or 5'bias necessarily inheres in the selection of exons for disposition on the genome-derived single exon microarrays of the present invention.

Conversely, the probes provided on the genome- derived single exon microarrays of the present invention typically, but need not necessarily, include intronic and/or intergenic sequence that is absent from EST microarrays, which are derived from mature mRNA.

Typically, at least about 50, 60, 70, 80 or 90% of the exon-including probes on the genome-derived single exon microarrays of the present invention include sequence drawn from noncoding regions. As discussed above, the additional presence of noncoding region does not significantly interfere with measurement of gene expression, and provides the additional opportunity to assay prespliced RNA, and thus measure such phenomena such as nuclear export control.

The genome-derived single exon microarrays of the

present invention are also quite different from in situ synthesis microarrays, where probe size is severely constrained by inadequacies in the photolithographic synthesis process.

Typically, probes arrayed on in situ synthesis microarrays are limited to a maximum of about 25 bp. As a well known consequence, hybridization to such chips must be performed at low stringency. In order, therefore, to achieve unambiguous sequence-specific hybridization results, the in situ synthesis microarray requires substantial redundancy, with concomitant programmed arraying for each probe of probe analogues with altered (i. e., mismatched) sequence.

In contrast, the longer probe length of the genome-derived single exon microarrays of the present invention allows much higher stringency hybridization and wash. Typically, therefore, exon-including probes on the genome-derived single exon microarrays of the present invention average at least about 100, 200, 300, 400 or 500 bp in length. By obviating the need for substantial probe redundancy, this approach permits a higher density of probes for discrete exons or genes to be arrayed on the microarrays of the present invention than can be achieved for in'situ synthesis microarrays.

A further distinction is that the probes in in situ synthesis microarrays typically are covalently linked to the substrate surface. In contrast, the probes disposed on the genome-derived microarray of the present invention typically are, but need not necessarily be, bound noncovalently to the substrate.

Furthermore, the short probe size on in situ microarrays causes large percentage differences in the melting temperature of probes hybridized to their complementary target sequence, and thus causes large percentage differences in the theoretically optimum

stringency across the array as a whole.

In contrast, the larger probe size in the microarrays of the present invention create lower percentage differences in melting temperature across the range of arrayed probes.

A further significant advantage of the microarrays of the present invention over in situ synthesized arrays is that the quality of each individual probe can be confirmed before deposition. In contrast, the quality of probes cannot be assessed on a probe-by-probe basis for the in situ synthesized microarrays presently being used.

The genome-derived single exon microarrays of the present invention are also distinguished over, and present substantial benefits over, the genome-derived microarrays from lower eukaryotes such as yeast. Lashkari et al., Proc. Natl. Acad. Sci. USA 94 : 13057-13062 (1997).

Only'about 220-250 of the 6100 or so nuclear genes in Saccharomyces cerevisiae-that is, only about 4 -5%-have standard, spliceosomal, introns, Lopez et al., Nucl. Acids Res. 28 : 85-86 (2000) ; Spingola et al., RNA 5 (2) : 221-34 (1999). Furthermore, the entire yeast genome has already been sequenced. These two facts permit the ready amplification and disposition of single-ORF amplicons on such microarray without the requirement for antecedent use of gene prediction and/or comparative sequence analyses.

Thus, a significant aspect of the present invention is the ability to identify and to confirm expression of predicted coding regions in genomic sequence drawn from eukaryotic organisms that have a higher percentage of genes having introns than do yeast such as Saccharomyces cerevisiae, particularly in genomic sequence drawn from eukaryotes in which at least about 10, 20 or 50% of protein-encoding genes have introns. In preferred

embodiments, the methods and apparatus of the present invention are used to identify and confirm expression of novel genes from genomic sequence of eukaryotes in which the average number of introns per gene is at least about one, two or three or more.

After the physical substrate is prepared, experimental verification of predicted function is performed.

In a preferred embodiment of the present invention, where the function sought to be identified in genomic sequence is protein coding, experimental verification is performed by measuring expression of the putative ORFs, typically through nucleic acid hybridization experiments, and in particularly preferred embodiments, through hybridization to genome-derived single exon microarrays prepared as above-described.

Expression is conveniently measured and expressed for each probe in the microarray as a ratio of the expression measured concurrently in a plurality of mRNA sources, according to techniques well known in the microarray art, Reviewed in Schena et al., and as further described in Example 2, below. The mRNA source for the reference against which specific expression is measured can 'be drawn from a homogeneous mRNA source, such as a single cultured cell-type, or alternatively can be heterogeneous, as from a pool of mRNA derived from multiple tissues and/or cell types, as further described in Example 2, infra. mRNA can be prepared by standard techniques, see Ausubel et al. and Maniatis et al., or purchased commercially. The mRNA is then typically reverse- transcribed in the presence of labeled nucleotides : the index source (that in which expression is desired to be measured) is reverse transcribed in the presence of nucleotides labeled with. a first label, typically a fluorophore (fluorochrome ; fluor ; fluorescent dye) ; the

reference source is reverse transcribed in the presence of a second label, typically a fluorophore, typically fluorometrically-distinguishable from the first label. As further described in Example 2, infra, Cy3 and Cy5 dyes prove particularly useful in these methods. After partial purification of the index and reference targets, hybridization to the probe array is conducted according to standard techniques, typically under a coverslip.

After wash, microarrays are conveniently scanned using a commercial microarray scanning device, such as a Gen3 Scanner (Molecular Dynamics, Sunnyvale, CA). Data on expression is then passed, with or without interim storage, to process 500, where the results for each probe are related to the original sequence.

Often, hybridization of target material to the genome-derived single exon microarray will identify certain of the probes thereon as of particular interest. Thus, it is often desirable that the user be able readily to obtain sufficient quantities of an individual probe, either for subsequent arrayed deposition upon an additional support substrate, often as part of a microarray having a plurality of probes so identified, or alternatively or additionally as a solitary solid-phase or solution-phase probe, for further use.

Thus, in another aspect, the present invention provides compositions and kits for the ready production of nucleic acids identical in sequence to, or substantially identical in sequence to, probes on the genome-derived single exon microarrays of the present invention.

In this aspect, a small quantity of each probe is disposed, typically without attachment to substrate, in a spatially-addressable ordered set, typically one per well of a microtiter dish. Although a 96 well microtiter plate can be used, greater efficiency is obtained using higher density arrays, such as are provided by microtiter plates

having 384, 864, 1536, 3456, 6144, or 9600 wells, and although microtiter plates having physical depressions (wells) are conveniently used, any device that permits addressable withdrawal of reagent from fluidly- noncommunicating areas can be used.

In this aspect of the invention, therefore, a fluidly noncommunicating addressable ordered set of individual probes, corresponding to those on a genome- derived single exon microarray, is provided, with each probe in sufficient quantity to permit amplification, such as by PCR. As earlier mentioned, the ORF-specific 5'primers used for genomic amplification can have a first common sequence added thereto, and the ORF-specific 3' primers used for genomic amplification can have a second, different, common sequence added thereto, thus permitting, in this preferred embodiment, the use of a single set of 5' and 3'primers to amplify any one of the probes from the amplifiable ordered set.

Each discrete amplifiable probe can also be packaged with amplification primers, solutes, buffers, etc., and can be provided in dry (e. g., lyophilized) form or wet, in the latter case typically with addition of agents that retard evaporation.

In another aspect of the present invention, a genome-derived single-exon microarray is packaged together with such an ordered set of amplifiable probes corresponding to the probes, or one or more subsets of probes, thereon. In alternative embodiments, the ordered set of amplifiable probes is packaged separately from the genome-derived single exon microarray.

In some embodiments, the microarray and/or ordered probe set are further packaged with recordable media that provide probe identification and addressing information, and that can additionally contain annotation information, such as gene expression data. Such recordable

media can be packaged with the microarray, with the ordered probe set, or with both.

If the microarray is constructed on a substrate that incorporates recordable media, such as is described in international patent application no. WO 98/12559, then separate packaging of the genome-derived single exon microarray and the bioinformatic information is not required.

The amount of amplifiable probe material should be sufficient to permit at least one amplification sufficient for subsequent hybridization assay.

Although the use of high density genome-derived microarrays on solid planar substrates is presently a preferred approach for the physical confirmation and characterization of the expression of sequences predicted to encode protein, other types of microarrays (as herein defined) can also be used.

Furthermore, as earlier mentioned, experimental verification of the function predicted from genomic sequence in process 200 can be bioinformatic, rather than, or additional to, physical verification.

For example, where the function desired to be identified is protein coding, the predicted ORFs can be compared bioinformatically to sequences known or suspected of being expressed.

Thus, the sequences output from process 300 (or process 200), can be used to query expression databases, such as EST databases, SNP ("single nucleotide polymorphism") databases, known cDNA and mRNA sequences, SAGE ("serial analysis of gene expression") databases, and more generalized sequence databases that allow query for expressed sequences. Such query can be done by any sequence query algorithm, such as BLAST ("basic local alignment search tool"). The results of such query- including information on identical sequences and

information on nonidentical sequences that have diffuse or focal regions of sequence homology to the query sequence- can then be passed directly to process 500, or used to inform analyses subsequently undertaken in process 200, process 300, or process 400.

Experimental data, whether obtained by physical or bioinformatic assay in process 400, is passed to process 500 where it is usefully related to the sequence data itself, a process colloquially termed"annotation". Such annotation can be done using any technique that usefully relates the functional information to the sequence, as, for example, by incorporating the functional data into the record itself, by linking records in a hierarchical or relational database, by linking to external databases, or by a combination thereof. Such database techniques are well within the skill in the art.

The annotated sequence data can be stored locally, uploaded to genomic sequence database 100, and/or displayed 800.

The methods and apparatus of the present invention rapidly produce functional information from genomic sequence. Coupled with the escalating pace at which sequence now accumulates, the rapid pace of sequence annotation produces a need for methods of displaying the information in meaningful ways.

FIG. 3 shows visual display 80 presenting a single genomic sequence annotated according to the present invention. Because of its nominal resemblance to artistic works of Piet Mondrian, visual display 80 is alternatively described herein as a"Mondrian".

Each of the visual elements of display 80 is aligned with respect to the genomic sequence being annotated (hereinafter, the"annotated sequence"). Given the number of nucleotides typically represented in an annotated sequence, representation of individual

nucleotides would rarely be readable in hard copy output of display 80. Typically, therefore, the annotated sequence is schematized as rectangle 89, extending from the left border of display 80 to its right border. By convention herein, the left border of rectangle 89 represents the first nucleotide of the sequence and the right border of rectangle 89 represents the last nucleotide of the sequence.

As further discussed below, however, the Mondrian visual display of annotated sequence can serve as a convenient graphical user interface for computerized representation, analysis, and query of information stored electronically. For such use, the individual nucleotides can conveniently be linked to the X axis coordinate of rectangle 89. This permits the annotated sequence at any point within rectangle 89 readily to be viewed, either automatically-for example, by time-delayed appearance of a small overlaid window upon movement of a cursor or other pointer over rectangle 89-or through user intervention, as by clicking a mouse or other pointing device at a point in rectangle 89.

Visual display 80 is generated after user specification of the genomic sequence to be displayed.

Such specification can consist of or include an accession number for a single clone (e. g., a single BAC accessioned into GenBank), wherein the starting and stopping nucleotides are thus absolutely identified, or alternatively can consist of or include an anchor or fulcrum point about which a chosen range of sequence is anchored, thus providing relative endpoints for the sequence to be displayed. For example, the user can anchor such a range about a given chromosomal map location, gene name, or even a sequence returned by query for similarity or identity to an input query sequence. When visual display 80 is used as a graphical user interface to

computerized data, additional control over the first and last displayed nucleotide will typically be dynamically selectable, as by use of standard zooming and/or selection tools.

Field 81 of visual display 80 is used to present the output from process 200, that is, to present the bioinformatic prediction of those sequences having the desired function within the genomic sequence. Functional sequences are typically indicated by at least one rectangle 83 (83a, 83b, 83c), the left and right borders of which respectively indicate, by their X-axis coordinates, the starting and ending nucleotides of the region predicted to have function.

Where a single bioinformatic method or approach identifies a plurality of regions having the desired function, a plurality of rectangles 83 is disposed horizontally in field 81. Where multiple methods and/or approaches are used to identify function, each such, method and/or approach can be represented by its own series of ; horizontally disposed rectangles 83, each such horizontally disposed series of rectangles offset vertically from those representing the results of the other methods and approaches.

Thus, rectangles 83a in FIG. 3 represent the functional predictions of a first method of a first approach for predicting function, rectangles 83b represent the functional predictions of a second method and/or second approach for predicting that function, and rectangles 83c represent the predictions of a third method and/or approach.

Where the function desired to be identified is protein coding, field 81 is used to present the bioinformatic prediction of sequences encoding protein.

For example, rectangles 83a can represent the results from GRAIL or GRAIL II, rectangles 83b can represent the results

from GENEFINDER, and rectangles 83c can represent the results from DICTION.

Optionally, and preferably, rectangles 83 collectively representing predictions of a single method and/or approach are identically colored and/or texture, and are distinguishable from the color and/or texture used for a different method and/or approach.

Alternatively, or in addition, the color, hue, density, or texture of rectangles 83 can be used further to report a measure of the bioinformatic reliability of the prediction. For example, many gene prediction programs will report a measure of the reliability of prediction.

Thus, increasing degrees of such reliability can be indicated, e. g., by increasing density of shading. Where display 80 is used as a graphical user interface, such measures of reliability, and indeed all other results output by the program, can additionally or alternatively be made accessible through linkage from individual rectangles 83, as by time-delayed window ("tool tip"window), or by pointer (e. g., mouse)-activated link.

As earlier described, increased predictive reliability can be achieved by requiring consensus among methods and/or approaches to determining function. Thus, field 81 can include a horizontal series of rectangles 83 that indicate one or more degrees of consensus in predictions-of function.

Although FIG. 3 shows three series of horizontally disposed rectangles in field 81, display 80 can include as few as one such series of rectangles and as many as can discriminably be displayed, depending upon the number of methods and/or approaches used to predict a given function.

Furthermore, field 81 can be used to show predictions of a plurality of different functions.

However, the increased visual complexity occasioned by such

display makes more useful the ability of the user to select a single function for display. When display 80 is used as a graphical user interface for computer query and analysis, such function can usefully be indicated and user- selectable, as by a series of graphical buttons or tabs (not shown in FIG. 3).

Rectangle 89 is shown in FIG. 3 as including interposed rectangle 84. Rectangle 84 represents the portion of annotated sequence for which predicted functional information has been assayed physically, with the starting and ending nucleotides of the assayed material indicated by the X axis coordinates of the left and right borders of rectangle 84. Rectangle 85, with optional inclusive circles 86 (86a, 86b, and 86c) displays the results of such physical assay.

Although a single rectangle 84 is shown in FIG.

3, physical assay is not limited to just one region of annotated genomic sequence. It is expected that an increasing percentage of regions predicted to have function by process 200 will be assayed physically, and that display 80 will accordingly, for any given genomic sequence, have an increasing number of rectangles 84 and 85, representing an increased density of sequence annotation.

Where the function desired to be identified is protein coding, rectangle 84 identifies the sequence of the probe used to measure expression. In embodiments of the present invention where expression is measured using genome-derived single exon microarrays, rectangle 84 identifies the sequence included within the probe immobilized on the support surface of the microarray. As noted supra, such probe will often include a small amount of additional, synthetic, material incorporated during amplification and designed to permit reamplification of the probe, which sequence is typically not shown in display 80.

Rectangle 87 is used to present the results of

bioinformatic assay of the genomic sequence. For example, where the function desired to be identified is protein coding, process 400 can include bioinformatic query of expression databases with the sequences predicted in process 200 to encode exons. And as earlier discussed, because bioinformatic assay presents fewer constraints than does physical assay, often the entire output of process 200 can be used for such assay, without further subsetting thereof by process 300. Therefore, rectangle 87 typically need not have separate indicators therein of regions submitted for bioinformatic assay ; that is, rectangle 87 typically need not have regions therein analogous to rectangles 84 within rectangle 89.

Rectangle 87 as shown in FIG. 3 includes smaller rectangles 880 and 88. Rectangles 880 indicate regions that returned a positive result in the bioinformatic assay, with rectangles 88 representing regions that did not return such positive results. Where the function desired to be predicted and displayed is protein coding, rectangles 880 indicate regions of the predicted exons that identify sequence with significant similarity in expression databases, such as EST, SNP, SAGE databases, with rectangles 88 indicating genes novel over those identified in existing expression data bases.

Rectangles 880 can further indicate, through color, shading, texture, or the like, additional information obtained from bioinformatic assay.

For example, where the function assayed and displayed is protein coding, the degree of shading of rectangles 880 can be used to represent the degree of sequence similarity found upon query of expression databases. The number of levels of discrimination can be as few as two (identity, and similarity, where similarity has a user-selectable lower threshold). Alternatively, as many different levels of discrimination can be indicated as

can visually be discriminated.

Where display 80 is used as a graphical user interface, rectangles 880 can additionally provide links directly to the sequences identified by the query of expression databases, and/or statistical summaries thereof.

As with each of the precedingly-discussed uses of display 80 as a graphical user interface, it should be understood that the information accessed via display 80 need not be resident on the computer presenting such display, which often will be serving as a client, with the linked information resident on one or more remotely located servers.

Rectangle 85 displays the results of physical assay of the sequence delimited by its left and right borders.

Rectangle 85 can consist of a single rectangle, thus indicating a single assay, or alternatively, and increasingly typically, will consist of a series of rectangles (85a, 85b, 85c) indicating separate physical assays of the same sequence.

Where the function assayed is gene expression, and where gene expression is assayed as herein described using simultaneous two-color fluorescent detection of hybridization to genome-derived single exon microarrays, individual rectangles 85 can be colored to indicate the degree of expression relative to control. Conveniently, shades of green can be used to depict expression in-the sample over control values, and shades of red used to depict expression less than control, corresponding to the spectra of the Cy3 and Cy5 dyes conventionally used for respective labeling thereof. Additional functional information can be provided in the form of circles 86 (86a, 86b, 86c), where the diameter of the circle can be used to indicate expression intensity. As discussed infra, such relative expression (expression ratios) and absolute

expression (signal intensity) can be expressed using normalized values.

Where display 80 is used as a graphical user interface, rectangle 85 can be used as a link to further information about the assay. For example, where the assay is one for gene expression, each rectangle 85 can be used to link to information about the source of the hybridized mRNA, the identity of the control, raw or processed data from the microarray scan, or the like.

FIG. 4 is rendition of display 80 representing gene prediction and gene expression for a hypothetical BAC, showing conventions used in the Examples presented infra.

BAC sequence ("Chip seq.") 89 is presented, with the physically assayed region thereof (corresponding to rectangle 84 in FIG. 3) shown in white. Algorithmic gene predictions are shown in field 81, with predictions by GRAIL shown, predictions by GENEFINDER, and predictions by DICTION shown. Within rectangle 87, regions of sequence that, when used to query expression databases, return identical or similar sequences ("EST hit") are shown as white rectangles (corresponding to rectangles 880 in FIG.

3), gray indicates low homology, and black indicates unknowns (where black and gray would correspond to rectangles 88 in FIG. 3).

Although FIGS. 3 and 4 show a single stretch of sequence, uninterrupted from left to right, longer sequences are usefully represented by vertical stacking of such individual Mondrians, as shown in FIGS. 9 and 10.

Single Exon Probes Useful For Measuring Gene Expression The methods and apparatus of the present invention rapidly produce functional information from genomic sequence. Where the function to be identified is protein coding, the methods and apparatus of the present

invention rapidly identify and confirm the expression of portions of genomic sequence that function to encode protein. As a direct result, the methods and apparatus of the present invention rapidly yield large numbers of single-exon nucleic acid probes, the majority from previously unknown genes, each of which is useful for measuring and/or surveying expression of a specific gene in one or more tissues or cell types.

It is, therefore, another aspect of the present invention to provide genome-derived single exon nucleic acid probes useful for gene expression analysis, and particularly for gene expression analysis by microarray.

Using the methods and genome-derived single-exon microarrays of the present invention, we have for example readily identified a large number of unique ORFs from human genomic sequence. Using single exon probes that encompass these ORFs, we have demonstrated, through microarray hybridization analysis, the expression of 5, 074 of these ORFs in HBL 100 cells.

The HBL 100 human breast cancer cell line was established in vitro from milk of an apparently healthy woman. The cells express a variant of SV40 large T antigen, and genomic DNA from HBL 100 cells possesses transforming activity associated with the viral information. The HBL100 cell line is nontumorigenic, and acquires the capacity to invade normal tissues and to replace them by proliferation in vitro only at high passage levels (HPL) ; these epithelial cells are thus are a useful model for studying breast tumor progression in vitro. HBL 100 cells bind both epidermal growth factor (EGF) and glucocorticoids but are progesterone receptor negative.

As would immediately be appreciated by one of skill in the art, each single exon probe having demonstrable expression in HBL 100 cells is currently available for use in measuring the level of its ORF's expression in Breast.

Diseases of the breast are a significant cause of human morbidity and mortality. Increasingly, genetic factors are being found that contribute to predisposition, onset, and/or aggressiveness of most, if not all, of these diseases. Although mutations in single genes have been identified as causative for some diseases of the breast, for the most part these disorders are believed to have polygenic etiologies.

For example, according to the American Cancer Society (ACS), carcinoma of the breast is the second most common cancer in women and, after lung cancer, is the second deadliest. The ACS estimates that in the U. S. there occurred 182, 800 new cases of malignant breast cancer in 2000, and about 40, 800 deaths from the disease. Although incidence of breast cancer is said to have declined, the disease clearly continues to represent a serious risk to the health and life of American women. Indeed, about one in nine U. S. women will develop breast cancer in her lifetime, and at present mortality rates, about a third of such women will eventually die from the disease.

A variety of factors are known to increase the risk of breast carcinoma. Sex is one : breast cancer in men is rare. Age is another : as women age, their risk for developing breast cancer increases, a 70 year old woman having three times the risk of developing cancer and five times the risk of dying from the disease as compared to a 40 year old woman. Most breast cancers occur after age 50, although in women with a genetic susceptibility, breast cancer tends to occur at an earlier age than in sporadic cases. Reproductive and menstrual history are also known to affect risk, with risk increasing with early menarche and late menopause, and is reduced by early first full term pregnancy. Additional risk factors, oft-times termed "lifestyle factors", include weight gain, obesity, fat' intake, alcohol consumption, and level of physical

activity.

That genetic factors underlie the etiology of breast cancer is suggested by the approximately two-fold increased risk for development of breast cancer by women with a first-degree relative who has also developed breast cancer. After gender and age, a positive family history is the strongest known predictive risk factor for breast cancer. Genetic linkage analysis in families with high rates of inherited cancer have facilitated the identification of several genes in which mutations can be shown to contribute substantially to the development and progression of breast cancer, including BRCA1, BRCA2, p53, and PTEN/MMAC1. Further study has made clear, however, that these genes are not alone sufficient to explain all genetic contributions to breast cancer.

For example, BRCA1 appears to be responsible for disease in up to 90% of families with both breast and ovarian cancer, but in only 45% of families with multiple cases of breast cancer without occurrence of ovarian cancer. And mutations in BRCA2, localized to the long arm of chromosome 13, are thought to account for only approximately 35% of multiple case breast cancer families.

Furthermore, despite the strong correlation between germline mutations in BRCA1 or BRCA2 and development of breast cancer, only weak connections have been made between these genes and sporadic breast cancer.

Epistatic effects of BRCA1 and BRCA2 mutations on other genetic loci, only some of which have been identified, have been postulated to account for some of the deleterious effects of mutations in these two genes.

Thus, mutations in p53 seem to be much more frequent in BRCA1 breast cancers (20/26) and somewhat more frequent in BRCA2-associated breast cancers (10/22) than in grade-matched sporadic cancers (7/20). BRCA mutation- associated cancers contain p53 mutations not typically

found in sporadic breast cancer, and 12 individual hereditary breast cancers have been shown to contain more than a single p53 mutation. Mutations of BRCA1 and BRCA2 may thus confer a"mutator"phenotype permitting the accumulation of genetic abnormalities, with p53 inactivation selected during tumor progression.

Additionally, genome-wide screening for chromosomal gains or losses in breast cancers harboring BRCA1 or BRCA2 mutations demonstrated more regions that were amplified or deleted compared to controls, suggesting a generalized increase in large-scale genomic instability.

Chromosomes 5q, 4q, and 4p had very frequent loss of heterozygosity in BRCA1 tumors, while BRCA2 tumors were characterized by losses at 13q (near the BRCA2 locus itself) and 6q, and chromosomal gains at 17q (outside of the HER2/neu locus) and 20q.

Mutations of other genes have also been implicated in susceptibility to development or aggressiveness of breast cancer. For example, germline mutations in the ATM gene, localized to chromosome llq22-23, result in an increased risk of breast cancer among female heterozygote carriers with an estimated relative risk of 3. 9 to 6. 4 ; it is unclear, however, if mutations in the ATM gene itself contribute to breast cancer.

Normal allelic variation in a variety of genes, as opposed to frank mutation, may also influence susceptibility to developing breast carcinoma and the propensity for the disease to progress. Such polymorphisms may thus explain why particular women or ethnic groups who do not otherwise bear mutations in genes known to be linked to breast cancer are at greater risk, especially in the context of exposure to environmental agents and other nonhereditary risk factors.

Polymorphically expressed genes may code for enzymes that

metabolize estrogens or detoxify drugs and environmental carcinogens.

For example, molecular epidemiologic studies of cancer of the breast have examined associations with p450 cytochrome genotypes including CYP1A1, CYP2D6, and CYP17.

The CYP1A1 gene, located on chromosome 15q, encodes the enzyme aryl hydrocarbon hydroxylase (AHH), present in breast tissue, and which catabolizes polycyclic aromatic hydrocarbons and arylamines. AHH is strongly inducible, i. e., greater enzymatic activity is seen with greater exposure to substrates. AHH catalyzes the monooxygenation of polycyclic aromatic hydrocarbons to phenolic products and epoxides that may be carcinogenic. AHH is also involved in the conversion of estrogen to hydroxylated conjugated estrogens such as 2-hydroxyestradiol.

Three polymorphisms in the CYP1A1 gene have been identified : an MspI RFLP of the 3'end of the gene (MspI) ; an adenine to guanine mutation in exon 7, causing an isoleucine to valine substitution (Ile-Val) ; and a polymorphism of the CYP1A1 gene identified among Negroids.

The frequencies of the MspI and Ile-Val polymorphisms vary considerably by race, being higher among Japanese and Hawaiian populations as compared with Caucasians and Negroids.

The CYP2D6 gene is located on chromosome 22q and encodes the enzyme debrisoquine hydroxylase, which metabolizes a variety of drugs and other xenobiotics. Like other polymorphically expressed p450 enzymes, it may activate procarcinogens or, conversely, detoxify carcinogens. A number of alleles have been characterized at the CYP2D6 locus. The"poor metabolizer"phenotype (CYP2D6 mutant/mutant genotype), which is rare in Asians, occurs in about 5% to 10% of Caucasians and in 2% of Negroids.

As another example, the N-acetyl transferase-1

(NAT1) and N-acetyl transferase-2 (NAT2) genes are located on chromosome 8q. Allelic variation in the NAT genes may contribute to variation in populations as to the susceptibility of individuals to development of breast carcinoma, particularly in the context of exposure to compounds present in tobacco. NAT2 detoxifies or, conversely, activates aromatic amines found in tobacco smoke such as 4-aminobiphenyl. Both phenotypic assays and genotypic assays for NAT2 can be used to classify individuals as rapid or slow acetylators. Genetic variants of the NAT2 gene have been cloned and 6 alleles at this locus have been identified : the Fl allele confers the fast acetylator phenotype. The distribution of NAT1 and NAT2 alleles differs widely between racial and ethnic groups.

As yet another example, the glutathione S-transferase-Ml (GSTM1) gene is located on chromosome 1 and the gene for glutathione S-transferase-T1 (GSTT1) is located on chromosome llq. A glutathione S-transferase-P1 (GSTP1) gene has also been identified.

Glutathione S-transferases detoxify a variety of carcinogens and cytotoxic drugs (for example, benzo (a) pyrene, monohalomethanes such as methyl chloride, ethylene oxide, pesticides, and solvents used in industry) by catalyzing the conjugation of a glutathione moiety to the substrate. Allelic variation in the glutathione-S- transferase genes may contribute to variation in populations as to the susceptibility of individuals to development of breast carcinoma, particularly in the context of. exposure to environmental toxins. Individuals homozygous for deletions in the GSTM1, GSTT1, or GSTP1 genes may have a higher risk of cancer of the breast and other sites because of their impaired ability to metabolize and eliminate carcinogens.

GSTM1 is polymorphically expressed and 3 alleles at the GSTM1 locus have been identified : GSTM1-0

(homozygous deletion genotype), GSTMla, and GSTMlb. The null allele (GSTM1-0) is present in about 38% to 67% of Caucasians and 22% to 35% of Negroids. GSTM is not expressed in breast tissue at high levels. Two functionally different genotypes at the GSTT1 locus have been described : GSTT1-0 (homozygous deletion genotype) and GSTT1-1 (genotypes with 1 or 2 undeleted alleles). A polymorphism of the GSTP1 gene, A313G (changing codon 105 from Ile to Val), has been identified. The GSTT1-0 allele has been associated with accelerated age of first breast cancer diagnosis as compared with the GSTT1-1 allele.

Many other genes have been suggested to be involved in the development and/or progression of breast cancer, either as a result of gain of function or loss of function mutations, or as a result of normal allelic variation within different populations. A nonexhaustive list of such genes, each followed by the gene's chromosomal location, if known, follows : AMPH 7pl4-pl3 ; AMPHL (BIN1, SH3P9) 2ql4 ; API4 (survivin, SW) 17q25 ( ?) ; ARHA (ARH12, RhoA) 3p21. 3 ; ARHC (RhoC) 1p21-p13 ; ATM (ATA, ATC) 11q22. 3 ; BAG1 9p12 ; BARD1 2q34-q35 ; BCARl 16q23. 1 ; BCAR2 ; BCAR3 (NSP2) ; BCAS1 (NABC1, AIBC1) 20ql3. 2-ql3. 3 ; BRCA1 17q21 ; BRCA2 13ql2. 3 ; CCND1 (D11S287E, Cyclin D, PRAD1) llql3 ; CD44 (MDU3, HA, MDU2) llpter-pl3 ; CD9 (p24, MIC3, BA2) 12pl3 ; CDKN1B (KIP1, P27) 12pl3 ; CDKN2A (P16, INK4A, MTS1) 9p21 ; COMT 22q11. 2 ; COT (MAP3K8, TPL-2, EST) 10p11. 2 ; CSK (c-src) 15q23-q25 ; CTSD (CPSD) llpl5. 5 ; CYP17 10q24. 3 ; CYP19 15q21. 1 ; CYP1A1 (CYP1) 15q22-q24 ; CYP1B1 (GLC3A) 2p22-p21 ; EFNB2 (EPLG5, LERK5, ephrin-B2) 13q33 ; EIF3S6 (INT6) 8q22-q23 ; EIF4E (EIF-4E) 4q21-q25 ; EMS1 llql3 ; ERBB2 (HER2, NEU) 17qll. 2-ql2 ; ERBB3 (HER3) 12ql3 ; ESR1 (ESRA) 6q25. 1 ; ESR2 (ESRB, ERBeta) 14q ; FGF8 (AIGF) 10q24 ; GSTM1 (GST1, MU) lpl3. 3 ; GSTP1 (FAEES3, GST3, PI) llql3 ; GSTT1 22qll. 23 ; HRAS llpl5. 5 ; HSPB1 (HSP27) 7q ; HSPCA (HSP90A, HSPC1) ; HSPCB (HSP90B, HSPC2) 6pl2 ; IGF1

12q22-q24. 1 ; IGF1R (JTK13) 15q25-q26 ; IGF2 llpl5. 5 ; IL6 (IFNB2) 7p21 ; ING1 13q34 ; KISS1 (KiSS-1) lq32 ; KLK3 (PSA, APS) 19ql3 ; LASP1 (MLN50) 17qll-q21. 3 ; LIBC 6q22 ; MAP2K4 (MKK4, SEK1, JNKK1) 17pll. 2 ; MKI67 (Ki-67) 10q25-qter ; MMP11 (STMY3, STR3) 22qll. 2 ; MMP2 (CLG4A, CLG4, GELA) 16ql3 ; MUC1 (PUM, PEM) lq21 ; MYC (CMYC, C-MYC) 8q24. 12-q24. 13 ; NOTCH4 (INT3, NOTCH3) 6p21. 3 ; PCNA 20pl2 ; PI5 (maspin) 18q21. 3 ; PLAU (uPA, URK) 10q24 ; PSEN2 (D21S21, HPS2, BCEI) 21q22. 3 ; RARB (NR1B2, HAP) 3p24 ; RB1 (Rb) 13ql4. 2 ; S100A4 (MTS1, P9KA, metastasin) lq21 ; SLC22A1L (BWSCRlA, ORCTL2, IMPT1) llpl5. 5 ; SNCG (BCSG1) 10q23. 2-q23. 3 ; SRD5A2 2p23 ; STIP1 (HSP70) 11 ? ; STK11 (LKBl, PJS) 19p13. 3 ; TFAP2A (AP2, AP2TF) 6p24 ; TFAP2B (AP2B) 6p12 ; TFAP2C 20q13. 2 ; TFF1 (D21S21, BCEI) 21q22. 3 ; TGFBR2 3p22 ; TIMP2 17q25 ; TP53 (p53, P53) 17q13. 1 ; TPD52 (D52) 8q21 ; TPD52L1 (D53, hD53) 6q22-q23 ; TSG101 llpl5. 2-pl5. 1.

The etiology of non-cancerous disorders of the breast may also involve genetic factors. Such disorders include disorders of development, inflammatory diseases of the breast, fibrocystic changes, proliferative breast disease, and non-carcinoma tumors.

Disorders of development of the breast include supernumerary nipples or breasts ; accessory axillary breast tissue ; congenital inversion of the nipples ; and macromastia.

Inflammatory diseases of the breast include acute mastitis ; periductal mastitis, also called recurrent subareolar absecess and sqamous metaplasia of the lactiferous ducts ; mammary duct ectasia ; fat necrosis ; and granulomatous mastitis, including granulomatous lobular mastitis. Systemic granulomatous diseases that can affect the breast include Wegener granulomatosis and sarcoidosis.

Proliferative breast diseases include epithelial hyperplasia ; sclerosing adenosis ; and small duct papillomas. Non-carcinoma tumors include stomal tumors

including fibroadenoma and phyllodes tumor, ana sarcomas that include angiosarcoma, rhabdomyosarcoma, liposarcoma, leiomyosarcoma, chondrosarcoma and osteosarcoma. Other breast tumors include epithelial cell tumors including large duct papillomas.

The human genome-derived single exon nucleic acid probes and microarrays of the present invention are useful for predicting, diagnosing, grading, staging, monitoring and prognosing diseases of human breast, particularly those diseases with polygenic etiology. With each of the single exon probes described herein shown to be expressed at detectable levels in human breast cancer cells, and with about 2/3 of the probes identifying novel genes, the single exon microarrays of the present invention provide exceptionally high informational content for such studies.

For example, diagnosis, grading, and/or staging of a disease can be based upon the quantitative relatedness of a patient gene expression profile to one or more reference expression profiles known to be characteristic of a given breast disease, or to specific grades or stages thereof.

In one embodiment, the patient gene expression profile is generated by hybridizing nucleic acids obtained directly or indirectly from transcripts expressed in the patient's breast to the genome-derived single exon microarray of the present invention. Reference profiles are obtained similarly by hybridizing nucleic acids from individuals with known disease. Methods for quantitatively relating gene expression profiles, without regard to the. function of the protein encoded by the gene, are disclosed in WO 99/58720, incorporated herein by reference in its entirety.

In another approach, the genome-derived single exon probes and microarrays of the present invention can be used to interrogate genomic DNA, rather than pools of

expressed message ; this latter approach permits predisposition to and/or prognosis of breast disease to be assessed through the massively parallel determination of altered copy number, deletion, or mutation in the patient's genome of exons known to be expressed in human breast. The algorithms set forth in WO 99/58720 can be applied to such genomic profiles without regard to the function of the protein encoded by the interrogated gene.

The utility is specific to the probe ; at sufficiently high hybridization stringency, which stringencies are well known in the art-see Ausubel et al. and Maniatis et al.-each probe reports the level of expression of message specifically containing that ORF.

It should be appreciated, however, that the probes of the present invention, for which expression in the HBL 100 cells has been demonstrated are useful for both measurement in the Breast and for survey of expression in other tissues.

Significant among such advantages is the presence of probes for novel genes.

As mentioned above and further detailed in Examples 1 and 2, the methods described enable ORFs which are not present in existing expression databases to be identified. And the fewer the number of tissues in which the ORF can be shown to be expressed, the more likely the ORF will prove to be part of a novel gene : as further discussed in Example 2, ORFs whose expression was measurable in only a single of the tested tissues were represented in existing expression databases at a rate of only 11%, whereas 36% of ORFs whose expression was measurable in 9 tissues were present in existing expression databases, and fully 45% of those ORFs expressed in all ten tested tissues were present in existing expressed sequence databases.

Either as tools for measuring gene expression or

tools for surveying gene expression, the genome-derived single exon probes of the present invention have significant advantages over the cDNA or EST-based probes that are currently available for achieving these utilities.

The genome-derived single exon probes of the present invention are useful in constructing genome-derived single exon microarrays ; the genome-derived single exon microarrays, in turn, are useful devices for measuring and for surveying gene expression in the human.

Gene expression analysis using microarrays- conventionally using microarrays having probes derived from expressed message-is well-established as useful in the biological research arts (see Lockhart et al. Nature 405, 827-836).

Microarrays have been used to determine gene expression profiles in cells in response to drug treatment (see, for example, Kaminski et al.,"Global Analysis of Gene Expression in Pulmonary Fibrosis Reveals Distinct Programs Regulating Lung Inflammation and Fibrosis,"Proc.

Natl. Acad. Sci. USA 97 (4) : 1778-83 (2000) ; Bartosiewicz et al.,"Development of a Toxicological Gene Array and Quantitative Assessment of This Technology,"Arch. Biochem.

Biophys. 376 (1) : 66-73 (2000)), viral infection (see for example, Geiss et al.,"Large-scale Monitoring of Host Cell Gene Expression During HIV-1 Infection Using cDNA Microarrays,"Virology 266 (1) : 8-16 (2000)) and during cell processes such as differentiation, senescence and apoptosis (see, for example, Shelton et al.,"Microarray Analysis of Replicative Senescence,"Curr. Biol. 9 (17) : 939-45 (1999) ; Voehringer et al.,"Gene Microarray Identification of Redox and Mitochondrial Elements That Control Resistance or Sensitivity to Apoptosis,"Proc. Natl. Acad. Sci. USA 97 (6) : 2680-5 (2000)).

Microarrays have also been used to determine abnormal gene expression in diseased tissues (see, for

example, Alon et al.,"Broad Patterns of Gene Expression Revealed by Clustering Analysis of Tumor and Normal Colon Tissues Probed by Oligonucleotide Arrays,"Proc. Natl.

Acad. Sci. USA 96 (12) : 6745-50 (1999) ; Perou et al., "Distinctive Gene Expression Patterns in Human Mammary Epithelial Cells and Breast Cancers, Proc. Natl. Acad. Sci.

USA 96 (16) : 9212-7 (1999) ; Wang et al.,"Identification of Genes Differentially Over-expressed in Lung Squamous Cell Carcinoma Using Combination of cDNA Subtraction and Microarray Analysis,"Oncogene 19 (12) : 1519-28 (2000) ; Whitney et al.,"Analysis of Gene Expression in Multiple Sclerosis Lesions Using cDNA Microarrays,"Ann. Neurol.

46 (3) : 425-8 (1999)), in drug discovery screens (see, for example, Scherf et al.,"A Gene Expression Database for the Molecular Pharmacology of Cancer,"Nat. Genet. 24 (3) : 236-44 (2000)) and in diagnosis to determine appropriate treatment strategies (see, for example, Sgroi et al.,"In vivo Gene Expression Profile. Analysis of Human Breast Cancer Progression,"Cancer Res. 59 (22) : 5656-61 (1999)).

In microarray-based gene expression screens of pharmacological drug candidates upon cells, each probe provides specific useful data. In particular, it should be appreciated that even those probes that show no change in expression are as informative as those that do change, serving, in essence, as negative controls.

For example, where gene expression analysis is used to assess toxicity of chemical agents on cells, the failure of the agent to change a gene's expression level is evidence that the drug likely does not affect the pathway of which the gene's expressed protein is a part.

Analogously, where. gene expression analysis is used to assess side effects of pharmacological agents-whether in lead compound discovery or in subsequent screening of lead compound derivatives-the inability of the agent to alter a gene's expression level is evidence that the drug does

not affect the pathway of which the gene's expressed protein is a part.

WO 99/58720 provides methods for quantifying the relatedness of a first and second gene expression profile and for ordering the relatedness of a plurality of gene expression profiles. The methods so described permit useful information to be extracted from a greater percentage of the individual gene expression measurements from a microarray than methods previously used in the art.

Other uses of microarrays are described in Gerhold et al., Trends Biochem. Sci. 24 (5) : 168-173 (1999) and Zweiger, Trends Biotechnol. 17 (11) : 429-436 (1999) ; Schena et al.

The invention particularly provides genome- derived single-exon probes known to be expressed in HBL 100 cells. The individual single exon probes can be provided in the form of substantially isolated and purified nucleic acid, typically, but not necessarily, in a quantity sufficient to perform a hybridization reaction.

Such nucleic acid can be in any form directly hybridizable to the message that contains the probe's ORF, such as double stranded DNA, single-stranded DNA complementary to the message, single-stranded RNA complementary to the message, or chimeric DNA/RNA molecules so hybridizable. The nucleic acid can alternatively or additionally include either nonnative nucleotides, alternative internucleotide linkages, or both, so long as complementary binding can be obtained. For example, probes can include phosphorothioates, methylphosphonates, morpholino analogs, and peptide nucleic acids (PNA), as are described, for example, in U. S. Patent Nos. 5, 142, 047 ; 5, 235, 033 ; 5, 166, 315 ; 5, 217, 866 ; 5, 184, 444 ; 5, 861, 250.

Usefully, however, such probes are provided in a form and quantity suitable for amplification, where the amplified product is thereafter to be used in the

hybridization reactions that probe gene expression.

Typically, such probes are provided in a form and quantity suitable for amplification by PCR or by other well known amplification technique. One such technique additional to PCR is rolling circle amplification, as is described, inter alia, in U. S. Patent Nos. 5, 854, 033 and 5, 714, 320 and international patent publications WO 97/19193 and WO 00/15779. As is well understood, where the probes are to be provided in a form suitable for amplification, the range of nucleic acid analogues and/or internucleotide linkages will be constrained by the requirements and nature of the amplification enzyme.

Where the probe is to be provided in form suitable for amplification, the quantity need not be sufficient for direct hybridization for gene expression analysis, and need be sufficient only to function as an amplification template, typically at least about 1, 10 or 100 pg or more.

Each discrete amplifiable probe can also be packaged with amplification primers, either in a single composition that comprises probe template and primers, or in a kit that comprises such primers separately packaged therefrom. As earlier mentioned, the ORF-specific 5 primers used for genomic amplification can have a first common sequence added thereto, and the ORF-specific 3' primers used for genomic amplification can have a second, different, common sequence added thereto, thus permitting, in this embodiment, the use of a single set of 5'and 3' primers to amplify any one of the probes. The probe composition and/or kit can also include buffers, enzyme, etc., required to effect amplification.

As mentioned earlier, when intended for use on a genome-derived single exon microarray of the present invention, the genome-derived single exon probes of the present invention will typically average at least about

100, 200, 300, 400 or 500 bp in length, including (and typically, but not necessarily centered about) the ORF.

Furthermore, when intended for use on a genome-derived single exon microarray of the present invention, the genome-derived single exon probes of the present invention will typically not contain a detectable label.

When intended for use in solution phase hybridization, however-that is, for use in a hybridization reaction in which the probe is not first bound to a support substrate (although the target may indeed be so bound)-length constraints that are imposed in microarray-based hybridization approaches will be relaxed, and such probes will typically be labeled.

In such case, the only functional constraint that dictates the minimum size of such probe is that each such probe must be capable of specifically identifying in a hybridization reaction the exon from which it is drawn. In theory, a probe of as little as 17 nucleotides is capable of uniquely identifying its cognate sequence in the human genome. For hybridization to expressed message-a subset of target sequence that is much reduced in complexity as compared to genomic sequence-even fewer nucleotides are required for specificity.

Therefore, the probes of the present invention can include as few as 20, 25 or 50 bp or ORF, or more. In particular embodiments, the ORF sequences are given in SEQ ID NOS. 5, 075-10, 058, respectively, for probe SEQ ID NOS.

1-5, 074. The minimum amount of ORF required to be included in the probe of the present invention in order to provide specific signal in either solution phase or microarray-based hybridizations can readily be determined for each of ORF SEQ ID NOS. 5, 075-10, 058 individually by routine experimentation using standard high stringency conditions.

Such high stringency conditions are described,

inter alia, in Ausubel et al. and Maniatis et al. For microarray-based hybridization, standard high stringency conditions can usefully be 50% formamide, 5X SSC, 0. 2 ug/pl poly (dA), 0. 2 ug/ul human Coti DNA, and 0. 5 % SDS, in a humid oven at 42C overnight, followed by successive washes of the microarray in 1X SSC, 0. 2% SDS at 55C for 5 minutes, and then 0. 1X SSC, 0. 2% SDS, at 55C for 20 minutes. For solution phase hybridization, standard high stringency conditions can usefully be aqueous hybridization at 65C in 6X SSC. Lower stringency conditions, suitable for cross-hybridization to mRNA encoding structurally-and functionally-related proteins, can usefully be the same as the high stringency conditions but with reduction in temperature for hybridization and washing to room temperature (approximately 25C).

When intended for use in solution phase hybridization, the maximum size of the single exon probes of the present invention is dictated by the proximity of other expressed exons in genomic DNA : although each single exon probe can include intergenic and/or intronic material contiguous to the ORF in the human genome, each probe of the present invention will include portions of only one expressed exon.

Thus, each single exon probe will include no more than about 25 kb of contiguous genomic sequence, more typically no more than about 20 kb of contiguous genomic sequence, more usually no more than about 15 kb, even more usually no more than about 10 kb. Usually, probes that are maximally about 5 kb will be used, more typically no more than about 3 kb.

It will be appreciated that the Sequence Listing appended hereto presents, by convention, only that strand of the probe and ORF sequence that can be directly translated reading from 5'to 3'end. As would be well understood by one of skill in the art, single stranded

probes must be complementary in sequence to the ORF as present in an mRNA ; it is well within the skill in the art to determine such complementary sequence. It will further be understood that double stranded probes can be used in both solution-phase hybridization and microarray-based hybridization if suitably denatured.

Thus, it is an aspect of the present invention to provide single-stranded nucleic acid probes that have sequence complementary to those described herein above and below, and double-stranded probes one strand of which has sequence complementary to the probes described herein.

The probes can, but need not, contain intergenic and/or intronic material that flanks the ORF, on one or both sides, in the same linear relationship to the ORF that the intergenic and/or intronic material bears to the ORF in genomic DNA. The probes do not, however, contain nucleic acid derived from more than one expressed ORF.

And when intended for use in solution hybridization, the probes of the present invention can usefully have detectable labels. Nucleic acid labels are well known in the art, and include, inter alia, radioactive labels, such as 3H, 32P, 33P, 35S, 125I, 131I; fluorescent labels, such as Cy3, Cy5, Cy5. 5, Cy7, SYBRe Green and other labels described in Haugland, Handbook of Fluorescent Probes and Research Chemicals, 7th ed., Molecular Probes Inc., Eugene, OR (2000), or fluorescence resonance energy transfer tandem conjugates thereof ; labels suitable for chemiluminescent and/or enhanced chemiluminescent detection ; labels suitable for ESR and NMR detection ; and labels that include one member of a specific binding pair, such as biotin, digoxigenin, or the like.

The probes, either in quantity sufficient for hybridization or sufficient for amplification, can be provided in individual vials or containers.

Alternatively, such probes can usefully be packaged as a plurality of such individual genome-derived single exon probes.

When provided as a collection of plural individual probes, the probes are typically made available in amplifiable form in a spatially-addressable ordered set, typically one per well of a microtiter dish. Although a 96 well microtiter plate can be used, greater efficiency is obtained using higher density arrays.

If, as earlier mentioned, the ORF-specific 5'primers used for genomic amplification had a first common sequence added thereto, and the ORF-specific 3' primers used for genomic amplification had a second, different, common sequence added thereto, a single set of 5'and 3'primers can be used to amplify all of the probes from the amplifiable ordered set.

Such collections of genome-derived single exon probes can usefully include a plurality of probes chosen for the common attribute of expression in the human HBL 100 cells.

In such defined subsets, typically at least 50, 60, 75, 80, 85, 90 or 95% or more of the probes will be chosen by their expression in the defined tissue or cell type.

The single exon probes of the present invention, as well as fragments of the single exon probes comprising selectively hybridizable portions of the probe ORF, can be used to obtain the full length cDNA that includes the ORF by (i) screening of cDNA libraries ; (ii) rapid amplification of cDNA ends ("RACE") ; or (iii) other conventional means, as are described, inter alia, in Ausubel et al. and Maniatis et al.

It is another aspect of the present invention to provide genome-derived single exon nucleic acid microarrays useful for gene expression analysis, where the term

: 1'- 71. n1 7i'3'tu ? : '6n. i1, i, i 'microarray"has the meaning given in the deinitio : '1 section of this description, supra.

The invention particularly provides genome- derived single-exon nucleic acid microarrays comprising a plurality of probes known to be expressed in human HBL 100 cells. In preferred embodiments, the present invention provides human genome-derived single exon microarrays comprising a plurality of probes drawn from the group consisting of SEQ ID NOS. : 1-5, 074.

When used for gene expression analysis, the genome-derived single exon microarrays provide greater physical informational density than do the genome-derived single exon microarrays that have lower percentages of probes known to be expressed commonly in the tested tissue.

At a fixed probe density, for example, a given microarray surface area of the defined subset genome-derived single exon microarray can yield a greater number of expression measurements. Alternatively, at a given probe density, the same number of expression measurements can be obtained from a smaller substrate surface area. Alternatively, at a fixed probe density and fixed surface area, probes can be provided redundantly, providing greater reliability in signal measurement for any given probe. Furthermore, with a higher percentage of probes known to be expressed in the assayed tissue, the dynamic range of the detection means can be adjusted to reveal finer levels discrimination among the levels of expression.

Although particularly described with respect to their utility as probes of gene expression, particularly as probes to be included on a genome-derived single exon microarray, each of the nucleic acids having SEQ ID NOS. : 1 -5, 074 contains an open-reading frame, set forth respectively in SEQ ID NOS. : 5, 075-10, 058, that encodes a protein domain. Thus, each of SEQ ID NOS. 1-5, 074 can be used, or that portion thereof in SEQ ID NOS. 5, 075-10, 058

based, to express a protein domain by standard in vitro recombinant techniques. See Ausubel et al. and Maniatis et al.

Additionally, kits are available commercially that readily permit such nucleic acids to be expressed as protein in bacterial cells, insect cells, or mammalian cells, as desired (e. g., HAT Protein Expression & Purification System, ClonTech Laboratories, Palo Alto, CA ; Adeno-M Expression System, ClonTech Laboratories, Palo Alto, CA ; Protein Fusion & Purification (pal) System, New England Biolabs, Beverley, MA) Furthermore, shorter peptides can be chemically synthesized using commercial peptide synthesizing equipment and well known techniques. Procedures are described, inter alia, in Chan et al. (eds.), Fmoc Solid Phase Peptide Synthesis : A Practical Approach (Practical Approach Series, (Paper)), Oxford Univ. Press (March 2000) (ISBN : 0199637245) ; Jones, Amino Acid and Peptide Synthesis (Oxford Chemistry Primers, No 7), Oxford Univ. Press (August 1992) (ISBN : 0198556683) ; and Bodanszky, Principles of Peptide Synthesis (Springer Laboratory), Springer Verlag (December 1993) (ISBN : 0387564314).

It is, therefore, another aspect of the invention to provide peptides comprising an amino acid sequence translated from SEQ ID NOS. : 5, 075-10, 058. Such amino acid sequences are set out in SEQ ID NOS : 10, 059-15, 009.

Any such recombinantly-expressed or synthesized peptide of at least 8, and preferably at least about 15, amino acids, can be conjugated to a carrier protein and used to generate antibody that recognizes the peptide. Thus, it is a further aspect of the invention to provide peptides that have at least 8, preferably at least 15, consecutive amino acids.

The following examples are offered by way of

illustration and not by way of limitation. EXAMPLE 1 Preparation of Single Exon Microarrays from ORFs Predicted in Human Genomic Sequence Bioinformatics Results All human BAC sequences in fewer than 10 pieces that had been accessioned in a five month period immediately preceding this study were downloaded from GenBank. This corresponds to-2200 clones, totaling-350 MB of sequence, or approximately 10% of the human genome.

After masking repetitive elements using the program CROSS MATCH, the sequence was analyzed for open reading frames using three separate gene finding programs.

The three programs predict genes using independent algorithmic methods developed on independent training sets : GRAIL uses a neural network, GENEFINDER uses a hidden Markoff model, and DICTION, a program proprietary to Genetics Institute, operates according to a different heuristic. The results of all three programs were used to create a prediction matrix across the segment of genomic DNA.

The three gene finding programs yielded a range of results. GRAIL identified the greatest percentage of genomic sequence as putative coding region, 2% of the data analyzed. GENEFINDER was second, calling 1%, and DICTION yielded the least putative coding region, with 0. 8% of genomic sequence called as coding region.

The consensus data were as follows. GRAIL and GENEFINDER agreed on 0. 7% of genomic sequence, GRAIL and DICTION agreed on 0. 5% of genomic sequence, and the three programs together agreed on 0. 25% of the data analyzed.

That is, 0. 25% of the genomic sequence was identified by all three of the programs as containing putative coding

region.

ORFs predicted by any two of the three programs ("consensus ORFs") were assorted into"gene bins"using two criteria : (1) any 7 consecutive exons within a 25 kb window were placed together in a bin as likely contributing to a single gene, and (2) all ORFs within a 25 kb window were placed together in a bin as likely contributing to a single gene if fewer than 7 exons were found within the 25 kb window. PCR The largest ORF from each gene bin that did not span repetitive sequence was then chosen for amplification, as were all consensus ORFs longer than 500 bp. This method approximated one exon per gene ; however, a number of genes were found to be represented by multiple elements.

Previously, we had determined that DNA fragments fewer than 250 bp in length do not bind well to the amino- modified glass surface of the slides used as support substrate for construction of microarrays ; therefore, amplicons were designed in the present experiments to approximate 500 bp in length.

Accordingly, after selecting the largest ORF per gene bin, a 500 bp fragment of sequence centered on the ORF was passed to the primer picking software, PRIMER3 (available online for use at http ://www-genome. wi. mit. edu/cgi-bin/primer/). A first additional sequence was commonly added to each ORF-unique 5'primer, and a second, different, additional sequence was commonly added to each ORF-unique 3'primer, to permit subsequent reamplification of the amplicon using a single set of"universal"5'and 3'primers, thus immortalizing the amplicon. The addition of universal priming sequences also facilitates sequence verification, and can be used to add a cloning site should some ORFs be found to warrant

further study.

The ORFs were then PCR amplified from genomic DNA, verified on agarose gels, and sequenced using the universal primers to validate the identity of the amplicon to be spotted in the microarray.

Primers were supplied by Operon Technologies (Alameda, CA). PCR amplification was performed by standard techniques using human genomic DNA (Clontech, Palo Alto, CA) as template. Each PCR product was verified by SYBRs green (Molecular Probes, Inc., Eugene, OR) staining of agarose gels, with subsequent imaging by Fluorimager (Molecular Dynamics, Inc., Sunnyvale, CA). PCR amplification was classified as successful if a single band appeared.

The success rate for amplifying ORFs of interest directly from genomic DNA using PCR was approximately 75%.

FIG. 5 graphs the distribution of predicted ORF (exon) length and distribution of amplified PCR products, with ORF length shown in red and PCR product length shown in blue (which may appear black in the figure). Although the range of ORF sizes is readily seen to extend to beyond 900 bp, the mean predicted exon size was only 229 bp, with a median size of 150 bp (n=9498). With an average amplicon size of 475 25 bp, approximately 50% of the average PCR amplification product contained predicted coding region, with the remaining 50% of the amplicon containing either intron, intergenic sequence, or both.

Using a strategy predicated on amplifying about 500 bp, it was found that long exons had a higher PCR failure rate. To address this, the bioinformatics process was adjusted to amplify 1000, 1500 or 2000 bp fragments from exons larger than 500 bp. This improved the rate of successful amplification of exons exceeding 500 bp, constituting about 9. 2% of the exons predicted by the gene finding algorithms.

Approximately 75% of the probes disposed on the array (90% of those that successfully PCR amplified) were sequence-verified by sequencing in both the forward and reverse direction using MegaBACE sequencer (Molecular Dynamics, Inc., Sunnyvale, CA), universal primers, and standard protocols.

Some genomic clones (BACs) yielded very poor PCR and sequencing results. The reasons for this are unclear, but may be related to the quality of early draft sequence or the inclusion of vector and host contamination in some submitted sequence data.

Although the intronic and intergenic material flanking coding regions could theoretically interfere with hybridization during microarray experiments, subsequent empirical results demonstrated that differential expression ratios were not significantly affected by the presence of noncoding sequence. The variation in exon size was similarly found not to affect differential expression ratios significantly ; however, variation in exon size was observed to affect the absolute signal intensity (data not shown).

The 350 MB of genomic DNA was, by the above- described process, reduced to 9750 discrete probes, which were spotted in duplicate onto glass slides using commercially available instrumentation (MicroArray GenII Spotter and/or MicroArray GenIII Spotter, Molecular Dynamics, Inc., Sunnyvale, CA). Each slide additionally included either 16 or 32 E. coli genes, the average hybridization signal of which was used as a measure of background biological noise.

Each of the probe sequences was BLASTed against the human EST data set, the NR data set, and SwissProt GenBank (May 7, 1999 release 2. 0. 9).

One third of the probe sequences (as amplified) produced an exact match (BLAST Expect ("E") values less

than 1 e~100) to either an EST (20% of sequences) or a known mRNA (13% of sequences). A further 22% of the probe sequences showed some homology to a known EST or mRNA (BLAST E values from 1 e-5 to 1 e~99). The remaining 45% of the probe sequences showed no significant sequence homology to any expressed, or potentially expressed, sequences present in public databases.

All of the probe sequences (as amplified) were then analyzed for protein similarities with the SwissProt database using BLASTX, Gish et al., Nature Genet. 3 : 266 (1993). The predicted functional breakdowns of the 2/3 of probes identical or homologous to known sequences are presented in Table 1. Table 1 Function of Predicted ORFs As Deduced From Comparative Sequence Analysis j Total V6 chip V7 chip Function Predicted from Comparative Sequence Analysis 211 96 115 Receptor 120 43 77'Zinc Finger 30 11 19 Homeobox 25 9 16 Transcription Factor Transcription 118 57 61 Structural 95 39 56 Kinase 36 18 18 Phosphatase 83 31 52 Ribosomal 45 19 26 Transport 21 17 14 Growth Factor 17 12 5 Cytochrome 50 33 17 Channel

As can be seen, the two most common types of genes were transcription factors and receptors, making up 2. 2% and 1. 8% of the arrayed elements, respectively. EXAMPLE 2 Gene Expression Measurements From Genome-Derived Single Exon Microarrays The two genome-derived single exon microarrays prepared according to Example 1 were hybridized in a series of simultaneous two-color fluorescence experiments to (1) Cy3-labeled cDNA synthesized from message drawn individually from each of brain, heart, liver, fetal liver, placenta, lung, bone marrow, HeLa, BT 474, or HBL 100 cells cells, and (2) Cy5-labeled cDNA prepared from message pooled from all ten tissues and cell types, as a control in each of the measurements. Hybridization and scanning were carried out using standard protocols and Molecular Dynamics equipment.

Briefly, mRNA samples were bought from commercial sources (Clontech, Palo Alto, CA and Amersham Pharmacia Biotech (APB)). Cy3-dCTP and Cy5-dCTP (both from APB) were incorporated during separate reverse transcriptions of 1 ug of polyA+ mRNA performed using 1 ug oligo (dT) 12-18 primer and 2 ug random 9mer primers as follows. After heating to 70C, the RNA : primer mixture was snap cooled on ice. After snap cooling on ice, added to the RNA to the stated final concentration was : 1X Superscript II buffer, 0. 01 M DTT, 100M dATP, 100 M dGTP, 100 uM dTTP, 50 M dCTP, 50 uM Cy3-dCTP or Cy5-dCTP 50 uM, and 200 U Superscript II enzyme. The reaction was incubated for 2 hours at 42C.

After 2 hours, the first strand cDNA was isolated by adding

1 U Ribonuclease H, and incubating for 30 minutes at 37C.

The reaction was then purified using a Qiagen PCR cleanup column, increasing the number of ethanol washes to 5.

Probe was eluted using 10 mM Tris pH 8. 5.

Using a spectrophotometer, probes were measured for dye incorporation. Volumes of both Cy3 and Cy5 cDNA corresponding to 50 pmoles of each dye were then dried in a Speedvac, resuspended in 30 pi hybridization solution containing 50% formamide, 5X SSC, 0. 2 pg/pl poly (dA), 0. 2 pg/pl human cotl DNA, and 0. 5 % SDS.

Hybridizations were carried out under a coverslip, with the array placed in a humid oven at 42C overnight. Before scanning, slides were washed in 1X SSC, 0. 2% SDS at 55C for 5 minutes, followed by 0. 1X SSC, 0. 2% SDS, at 55C for 20 minutes. Slides were briefly dipped in water and dried thoroughly under a gentle stream of nitrogen.

Slides were scanned using a Molecular Dynamics Gen3 scanner, as described. Schena (ed.), Microarray Biochip : Tools and Technology, Eaton Publishing Company/BioTechniques Books Division (2000) (ISBN : 1881299376).

Although the use of pooled cDNA as a reference permitted the survey of a large number of tissues, it attenuates the measurement of relative gene expression, since every highly expressed gene in the tissue/cell type- specific fluorescence channel will be present to a level of at least 10% in the control channel. Because of this fact, both signal and expression ratios (the latter hereinafter, "expression"or"relative expression") for each probe were normalized using the average ratio or average signal, respectively, as measured across the whole slide.

Data were accepted for further analysis only when signal was at least three times greater than biological noise, the latter defined by the average signal produced by

the E. coli control genes.

The relative expression signal for these probes was then plotted as function of tissue or cell type, and is presented in FIG. 6.

FIG. 6 shows the distribution of expression across a panel of ten tissues. The graph shows the number of sequence-verified products that were either not expressed ("0"), expressed in one or more but not all tested tissues ("1"-"9"), and expressed in all tissues tested ("10").

Of 9999 arrayed elements on the two microarrays (including positive and negative controls and"failed" products), 2353 (51%) were expressed in at least one tissue or cell type. Of the gene elements showing significant signal-where expression was scored as"significant"if the normalized Cy3 signal was greater than 1, representing signal 5-fold over biological noise (0. 2)-39% (991) were expressed in all 10 tissues. The next most common class (15%) consisted of gene elements expressed in only a single tissue.

The genes expressed in a single tissue were further analyzed, and the results of the analyses are compiled in FIG. 7.

FIG. 7A is a matrix presenting the expression of all verified sequences that showed expression greater than 3 in at least one tissue. Each clone is represented by a column in the matrix. Each of the 10 tissues assayed is represented by a separate row in the matrix, and relative expression of a clone in that tissue is indicated at the respective node by intensity of green shading, with the intensity legend shown in panel B. The top row of the matrix ("EST Hit") contains"bioinformatic"rather than "physical"expression data-that is, presents the results returned by query of EST, NR and SwissProt databases using the probe sequence. The legend for"bioinformatic

expression" (i. e., degree of homology returned) is presented in panel C. Briefly, white is known, black is novel, with gray depicting nonidentical with significant homology (white : E values < le-100 ; gray : E values from le- 05 to le-99 ; black : E values > le-05).

As FIG. 7 readily shows, heart and brain were demonstrated to have the greatest numbers of genes that were shown to be uniquely expressed in the respective tissue. In brain, 200 uniquely expressed genes were identified ; in heart, 150. The remaining tissues gave the following figures for uniquely expressed genes : liver, 100 ; lung, 70 ; fetal liver, 150 ; bone marrow, 75 ; placenta, 100 ; HeLa, 50 ; HBL, 100 ; and BT474, 50.

It was further observed that there were many more "novel"genes among those that were up-regulated in only one tissue, as compared with those that were down-regulated in only one tissue. In fact, it was found that ORFs whose expression was measurable in only a single of the tested tissues were represented in sequencing databases at a rate of only 11%, whereas 36% of the ORFs whose expression was measurable in 9 of the tissues were present in public databases. As for those ORFs expressed in all ten tissues, fully 45% were present in existing expressed sequence databases. These results are not unexpected, since genes expressed in a greater number of tissues have a higher likelihood of being, and thus of having been, discovered by EST approaches. Comparison of Signal from Known and Unknown Genes P g The normalized signal of the genes found to have high homology to genes present in the GenBank human EST database were compared to the normalized signal of those genes not found in the GenBank human EST database. The data are shown in FIG. 8.

FIG. 8 shows the normalized Cy3 signal intensity

for all sequence-verified products with a BLAST Expect ("E") value of greater than le-30 (designated"unknown") upon query of existing EST, NR and SwissProt databases, and shows in blue the normalized Cy3 signal intensity for all sequence-verified products with a BLAST Expect value of less than le-30 ("known"). Note that biological background noise has an averaged normalized Cy3 signal intensity of 0. 2.

As expected, the most highly expressed of the ORFs were"known"genes. This is not surprising, since very high signal intensity correlates with very commonly- expressed genes, which have a higher likelihood of being found by EST sequence.

However, a significant point is that a large number of even the high expressers were"unknown". Since the genomic approach used to identify genes and to confirm their expression does not bias exons toward either the 3' or 5'end of a gene, many of these high expression genes will not have been detected in an end-sequenced cDNA library.

The significant point is that presence of the gene in an EST database is not a prerequisite for incorporation into a genome-derived microarray, and further, that arraying such"unknown"exons can help to assign function to as-yet undiscovered genes.

Verification of Gene Expression To ascertain the validity of the approach described above to identify genes from raw genomic sequence, expression of two of the probes was assayed using reverse transcriptase polymerase chain reaction (RT PCR) and northern blot analysis.

Two microarray probes were selected on the basis of exon size, prior sequencing success, and tissue-specific gene expression patterns as measured by the microarray

experiments. The primers originally used to amplify the two respective ORFs from genomic DNA were used in RT PCR against a panel of tissue-specific cDNAs (Rapid-Scan gene expression panel 24 human cDNAs) (OriGene Technologies, Inc., Rockville, MD).

Sequence AL079300-1 was shown by microarray hybridization to be present in cardiac tissue, and sequence AL031734_1 was shown by microarray experiment to be present in placental tissue (data not shown). RT-PCR on these two sequences confirmed the tissue-specific gene expression as measured by microarrays, as ascertained by the presence of a correctly sized PCR product from the respective tissue type cDNAs.

Clearly, all microarray results cannot, and indeed should not, be confirmed by independent assay methods, or the high throughput, highly parallel advantages of microarray hybridization assays will be lost. However, in addition to the two RT-PCR results presented above, the observation that 1/3 of the arrayed genes exist in expression databases provides powerful confirmation of the power of our methodology-which combines bioinformatic prediction with expression confirmation using genome- derived single exon microarrays-to identify novel genes from raw genomic data.

To verify that the approach further provides correct characterization of the expression patterns of the identified genes, a detailed analysis was performed of the microarrayed sequences that showed high signal in brain.

For this latter analysis, sequences that showed high (normalized) signal in brain, but which showed very low (normalized) signal (less than 0. 5, determined to be biological noise) in all other tissues, were further studied. There were 82 sequences that fit these criteria, approximately 2% of the arrayed elements. The 10 sequences showing the highest signal in brain in microarray hybridizations are detailed in Table 2, along with assigned function, if known or reasonably predicted. Table 2 Function of the Most Highly Expressed Genes Expressed Only in Brain Microarray Normal Expressi Homology Gene Function Sequence ized on Ratio to EST as described by Name Signal present GenBank in GenBank AP000217-1 5. 2 +7. 7 High S-100 protein, b-chain, Ca2+ binding protein expressed in central nervous system t AP000047-1 2. 3 High Unknown Function AC006548-9 1. 7 High Similar to mouse membrane glyco-protein M6, expressed in central nervous system AC007245-5 1. 5 High Similar to amphiphysin, a synaptic vesicle- associated protein. Ref 21 L44140-4 1. 2 +2. 0 High Endothelial actin-binding protein found in nonmuscle filamin AC004689-9 1. 2 +3. 5 High Protein Phosphatase PP2A, neuronal/ downregulates activated protein kinases AL031657-1 1. 2 +3. 0 High Unknown function/ Contains the anhyrin motif, a common protein sequence motif AC009266-2 1. 1 +3. 7 Low Low homology to the Synaptotagmin I protein in rat/present at low levels throughout rat brain AP000086-1 1. 0 +2. 7 Low Unknown, very poor homology to collagen AC004689-3 1. 0 High Protein Phosphatase PP2A, neuronal/ downregulates activated protein kinases Of the ten sequences studied by these latter

confirmatory approaches, eight were previously known. Of these eight, six had previously been reported to be important in the central nervous system or brain. The exon giving the highest signal (AP00217-1) was found to be the gene encoding an S100B Ca2+ binding protein, reported in the literature to be highly and uniquely expressed in the central nervous system. Heizmann, Neurochem. Res. 9 : 1097 (1997).

A number of the brain-specific probe sequences (including AC006548-9, AC009266-2) did not have homology to any known human cDNAs in GenBank but did show homology to rat and mouse cDNAs. Sequences AC004689-9 and AC004689-3 were both found to be phosphatases present in neurons (Millward et al., Trends Biochem. Sci. 24 (5) : 186-191 (1999)). Two microarray sequences, AP000047-1 and AP000086-1 have unknown function, with AP000086-1 being absent from GenBank. Functionality can now be narrowed down to a role in the central nervous system for both of these genes, showing the power of designing microarrays in this fashion.

Next, the function of the chip sequences with the highest (normalized) signal intensity in brain, regardless of expression in other tissues, was assessed. In this latter analysis, we found expression of many more common genes, since the sequences were not limited to those expressed only in brain. For example, looking at the 20 highest signal intensity spots in brain, 4 were similar to tubulin (AC00807905 ; AF146191-2 ; AC007664-4 ; AF14191-2), 2 were similar to actin (AL035701-2 ; AL034402-1), and 6 were found to be homologous to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (AL035604-1 ; Z86090-1 ; AC006064-L, AC006064-K ; AC035604-3 ; AC006064-L). These genes are often used as controls or housekeeping genes in microarray experiments of all types.

Other interesting genes highly expressed in brain

were a ferritin heavy chain protein, which is reported in the literature to be found in brain and liver (Joshi et al., J. Neurol. Sci. 134 (Suppl) : 52-56 (1995)), a result duplicated with the array. Other highly expressed chip sequences included a translation elongation factor 12 (AC007564-4), a DEAD-box homolog (AL023804-4), and a Y- chromosome RNA-binding motif (Chai et al., Genomics 49 (2) : 283-89 (1998)) (AC007320-3). A low homology analog (AP00123-1/2) to a gene, DSCR1, thought to be involved in trisomy 21 (Down's syndrome), showed high expression in both brain and heart, in agreement with the literature (Fuentes et al., Mol. Genet. 4 (10) : 1935-44 (1995)).

As a further validation of the approach, we selected the BAC AC006064 to be included on the array.

This BAC was known to contain the GAPDH gene, and thus could be used as a control for the ORF selection process.

The gene finding and exon selection algorithms resulted in choosing 25 exons from BAC AC006064 for spotting onto the array, of which four were drawn from the GAPDH gene. Table 3 shows the comparison of the average expression ratio for the 4 exons from BAC006064 compared with the average expression ratio for 5 different dilutions of a commercially available GAPDH cDNA (Clontech). Table 3 Comparison of Expression Ratio, for each tissue, of GAPDH AC006064 (n = 4) Control (n = 5) Bone Marrow-1. 81 0. 11-1. 85 0. 08 Brain-1. 41 0. 11-1. 17 0. 05 BT474 1. 85 0. 09 1. 66 0. 12 Fetal Liver-1. 62 0. 07-1. 41 0. 05 HBL100 1. 32 0. 05 2. 64 0. 12 Heart 1. 16 0. 09 1. 56 0. 10 HeLa 1. 11 0. 06 1. 30 0. 15 Liver-1. 62 + 0. 22-2. 07 Lung -4.95 # 0.93 -3.75 # 0.21 Placenta-3. 56 0. 25-3. 52 0. 43

Each tissue shows excellent agreement between the experimentally chosen exons and the control, again demonstrating the validity of the present exon mining approach. In, addition, the data also show the variability of expression of GAPDH within tissues, calling into question its classification as a housekeeping gene and utility as a housekeeping control in microarray experiments. EXAMPLE 3 Representation of Sequence and Expression Data as a "Mondrian" For each genomic clone processed for microarray as above-described, a plethora of information was accumulated, including full clone sequence, probe sequence within the clone, results of each of the three gene finding programs, EST information associated with the probe sequences, and microarray signal and expression for multiple tissues, challenging our ability to display the information.

Accordingly, we devised a new tool for visual display of the sequence with its attendant annotation which, in deference to its visual similarity to the paintings of Piet Mondrian, is hereinafter termed a "Mondrian". FIGS. 3 and 4 present the key to the information presented on a Mondrian.

FIG. 9 presents a Mondrian of BAC AC008172 (bases

25, 000 to 130, 000 shown), containing the carbamyl phosphate synthetase gene (AF154830. 1). Purple background within the region shown as field 81 in FIG. 3 indicates all 37 known exons for this gene.

As can be seen, GRAIL II successfully identified 27 of the known exons (73%), GENEFINDER successfully identified 37 of the known exons (100%), while DICTION identified 7 of the known exons (19%).

Seven of the predicted exons were selected for physical assay, of which 5 successfully amplified by PCR and were sequenced. These five exons were all found to be from the same gene, the carbamyl phosphate synthetase gene (AF154830. 1).

The five exons were arrayed, and gene expression measured across 10 tissues. As is readily seen in the Mondrian, the five chip sequences on the array show identical expression patterns, elegantly demonstrating the reproducibility of the system.

FIG. 10 is a Mondrian of BAC AL049839. We selected 12 exons from this BAC, of which 10 successfully sequenced, which were found to form between 5 and 6 genes.

Interestingly, 4 of the genes on this BAC are protease inhibitors. Again, these data elegantly show that exons selected from the same gene show the same expression patterns, depicted below the red line. From this figure, it is clear that our ability to find known genes is very good. A novel gene is also found from 86. 6 kb to 88. 6 kb, upon which all the exon finding programs agree. We are confident we have two exons from a single gene since they show the same expression patterns and the exons are proximal to each other. Backgrounds in the following colors indicate a known gene (top to bottom) : red = kallistatin protease inhibitor (P29622) ; purple = plasma serine protease inhibitor (P05154) ; turquoise = al anti-chymotrypsin (P01011) ; mauve = 40S

ribosomal protein (P08865). Note that chip sequence 8 and 12 did not sequence verify. EXAMPLE 4 Genome-Derived Single Exon Probes Useful For Measuring Human Gene Expression The protocols set forth in Examples 1 and 2, supra, were applied to additional human genomic sequence as it became newly available in GenBank to identify unique exons in the human genome that could be shown to be expressed at significant levels in HBL 100 cells.

These unique exons are within longer probe sequences. Each probe was completely sequenced on both strands prior to its use on a genome-derived single exon microarray ; sequencing confirms the exact chemical structure of each probe. An added benefit of sequencing is that it placed us in possession of a set of single base- incremented fragments of the sequenced nucleic acid, starting from the sequencing primer 3'OH. (Since the single exon probes were first obtained by PCR amplification from genomic DNA, we were of course additionally in possession of an even larger set of single base incremented fragments of each of the 5, 074 single exon probes, each fragment corresponding to an extension product from one of the two amplification primers.) The structures of the 5, 074 unique single exon probes are clearly presented in the Sequence Listing as SEQ ID Nos. : 1-5, 074. The 16 nt 5'primer sequence and 16 nt 3'primer sequence present on the amplicon are not included in the sequence listing. The sequences of the exons present within each of these probes is presented in the Sequence Listing as SEQ ID Nos. : 5, 075-10, 058, respectively. It will be noted that some amplicons have

more than one exon, some exons are contained in more than one amplicon.

As detailed in Example 2, expression was demonstrated by disposing the amplicons as single exon probes on nucleic acid microarrays and then performing two- color fluorescent hybridization analysis ; significant expression is based on a statistical confidence that the signal is significantly greater than negative biological control spots. The negative biological control is formed from spotted DNA sequences from a different species. Here, 32 sequences from E. Coli were spotted in duplicate to give a total of 64 spots.

For each hybridisation (each slide, each colour) the median value of the signal from all of the spots is determined. The normalised signal value is the arithmetic mean of the signal from duplicate spots divided by the population median.

Control spots are eliminated if there is more that a five-fold difference between each one of the duplicate spots raw signals.

The median of the signal from the remaining control spots is calculated and all subsequent calculations are done with normalised signals.

Control spots having a signal of greater than median + 2. 4 (the value 2. 4 is roughly 12 times the observed standard deviation of control spot populations) are eliminated. Spots with such high signals are considered to be"outliers".

The mean and standard deviation of the modified control spot populations are calculated.

The mean + 3x the standard deviation (mean + (3*SD)) is used as the signal threshold qualifier for that particular hybridisation. Thus, individual thresholds are determined for each channel and each hybridisation.

This means that, assuming that the data is

distributed normally, there is a 99% confidence that any signal exceeding the threshold is significant.

The probes and their expression data are presented in Table 4, set forth respectively in Example 5.

Example 5 presents the subset of probes that is significantly expressed in the human HBL 100 cells and thus presents the subset of probes that was recognized to be useful for measuring expression of their cognate genes in human HBL 100 cells.

The sequence of each of the exon probes identified by SEQ ID NOS. : 5, 075-10, 058 was individually used as a BLAST (or, for SWISSPROT, BLASTX) query to identify the most similar sequence in each of dbEST, SwissProt (BLASTX), and NR divisions of GenBank. Because the query sequences are themselves derived from genomic sequence in GenBank, only nongenomic hits from NR were scored.

The smallest in value of the BLAST (or BLASTX) expect ("E") scores for each query sequence across the three database divisions was used as a measure of the "expression novelty"of the probe's ORF. Table 4 is sorted in descending order based on this measure, reported as "Most Similar (top) Hit BLAST E Value". Those sequences for which no"Hit E Value"is listed are those exons which were found to have no similar sequences.

As sorted, Table 4 thus lists its respective probes (by"AMPLICON SEQ ID NO. :" and additionally by the SEQ ID NO :. of the exon contained within the probe :"EXON SEQ ID NO. :") from least similar to sequences known to be expressed (i. e., highest BLAST E value), at the beginning of the table, to most similar to sequences known to be expressed (i. e., lowest BLAST E value), at the bottom of the table.

Table 4 further provides, for each listed probe, the accession number of the database sequence that yielded

the"Most Similar (top) Hit BLAST E Value", along with the name of the database in which the database sequence is found ("Top Hit Database Source").

Table 4 further provides SEQ ID NOS. corresponding to the predicted amino acid sequences where they have been determined for the probe and exon nucleotide sequences. These are set out as PEPTIDE SEQ ID NOS. :. The peptide sequences for a given exon are predicted as follows : Since each chip exon is a consensus sequence drawn from predictions from various exon finding programs (i. e.

Grail, GeneFinder and GenScan), the multiple initial ORFs are first determined in a uniform way according to each prediction. In particular, the reading frame for predicting the first amino acid in the peptide sequence always starts with the first base of any codon and ends with the last base of non-termination codon. Next, for each strand of the exon, initial ORFs are merged into one or more final ORFs in an exhaustive process based on the following criteria : 1) the merging ORFs must be overlapping, and 2) the merging ORFs must be in the same frame.

The Sequence Listing, which is a superset of all of the data presented in Table 4, further includes, for each probe, the most similar hit, with accession number and BLAST E value, from the each of the three queried databases.

Table 4 further lists, for each probe, a portion of the descriptor for the top hit ("Top Hit Descriptor") as provided in the sequence database. For those ORFs that are similar in sequence, but nonidentical to known sequences (e. g., those with BLAST E values between about le-05 and le-100), the descriptor reveals the likely function of the protein encoded by the probe's ORF.

Using BLAST E value cutoffs of le-05 (i. e., 1 x 10-5) and le-100 (i. e., 1 x 10-l00) as evidence of similarity to sequences known to be expressed is of course arbitrary :

in Example 2, supra, a BLAST E value of le-30 was used as the boundary when only two classes were to be defined for analysis (unknown, >le-30 ; known <le-30) (see also FIG. 8).

Furthermore, even when the"Most Similar (Top) Hit BLAST E Value is low, e. g., less than about le-100-which is probative evidence that the query sequence has previously<BR> <BR> <BR> <BR> <BR> <BR> <BR> been shown to be expressed-the top hit is highly unlikely exactly to match the probe sequence.

First, such expression entries typically will not have the intronic and/or intergenic sequence present within the single exon probes listed in the Table. Second, even the ORF itself is unlikely in such cases to be present identically in the databases, since most of the EST and mRNA clones in existing databases include multiple exons, without any indication of the location of exon boundaries.

As noted, the data presented in Table 4 represent a proper subset of the data present within the attached sequence listing. For each amplicon probe (SEQ ID NOs. : 1 -5, 074) and probe exon (SEQ ID NOs. : 5, 075-10, 058, respectively), the sequence listing further provides, through iterated annotation fields <220> and <223> : (a) the accession number of the BAC from which the sequence was derived ("MAP TO"), thus providing a link to the chromosomal map location and other information about the genomic milieu of the probe sequence ; (b) the most similar sequence provided by BLAST query of the EST database, with accession number and BLAST E value for the"hit" ; (c) the most similar sequence provided by BLAST query of the GenBank NR database, with accession number and BLAST E value for the"hit" ; and (d) the most similar sequence provided by BLASTX query of the SWISSPROT database, with accession number and BLAST E value for the"hit".

EXAMPLE 5 Genome-Derived Single Exon Probes Useful For Measuring Expression of Genes in Human HBL 100 cells Table 4 (209 pages) presents expression, homology, and functional information for the genome-derived single exon probes that are expressed significantly in human HBL 100 cells, a hormone sensitive human breast cancer cell line. Page 1 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database ID NO: Signal BLAST E No Top Hit Descriptor NO: NO: Source Value 447 5484 10501 4.37 878 5896 10937 8. 5 1028 6038 2. 62 1283 6282 11324 8. 84 1577 6574 11636 3. 1 1596 6592 11653 4. 71 1687 6683 11758 2. 18 1710 6705 11781 1. 16 1717 6712 11789 7. 06 1850 6839 11928 1.12 1933 6919 12018 1. 75 2101 7082 12197 1. 88 2216 7193 12315 4. 79 3112 8128 13148 3. 32 3366 8374 13394 1. 09 3437 8445 13471 12. 48 3481 8489 1. 38 3573 8580 13586 1. 16 3844 8846 1. 1 4074 9068 14058 1. 73 4139 9134 14117 6. 13 4211 9204 1. 28 4689 9674 14657 1. 1 4879 9858 14829 4. 14 4888 9867 14837 1. 25 2595 7558 12672 0. 93 9. 4E+00 L11433. 1 NT Dengue virus type 3 membrane protein (prM/M)/envelope glycoprotein (E) polyprotein mRNA, partial cds 2595 7558 12673 0. 93 9. 4E+00 L11433. 1 NT Dengue virus type 3 membrane protein (prM/M)/envelope glycoprotein (E) polyprotein mRNA, partial cds 2853 7873 12888 3. 02 9. 4E+00 AB043785. 1 NT Mus musculus AT3 gene for antithrombin, complete cds 437 5475 10491 2. 23 8. 4E+00 5031804 NT Homo sapiens insulin receptor substrate 1 (IRS1) mRNA 2909 7928 12946 3. 48 7. 2E+00 L12051. 1 NT Lycopersicon esculentum Mill. GTPase (SAR2) mRNA, complete cds 2909 7928 12947 3. 48 7. 2E+00 L12051. 1 NT Lycopersicon esculentum Mill. GTPase (SAR2) mRNA, complete cds 3445 8453 0. 71 5. 8E+00 7661557 NT Homo sapiens DESC1 protein (DESC1), mRNA Page 2 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database ID NO: Signal BLAST E No Top Hit Descriptor NO: NO: Source Value 4640 9625 14617 1.32 5.3E+00 L43126.1 NT Bovine immunodeficiency-like virus surface envelope gene, 5' end of cds 3949 8947 11.8 4.8E+00 AF185255.1 NT Eunice australis histone H3 (H3) gene, partial cds 287 5345 10357 2. 26 4. 7E+00 BF240552. 1 EST_HUMAN 601875654F1 NIH_MGC_55 Homo sapiens cDNA clone IMAGE : 4099716 5' 288 5345 10357 1.89 4.7E+00 BF240552. 1 EST_HUMAN 601875654F1 NIH_MGC_55 Homo sapiens cDNA clone IMAGE : 4099716 5' 3200 8216 13237 1. 53 4. 7E+00 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21C080 3469 8477 1. 24 4.0E+00 P38229 SWISSPROT GLC7-INTERACTING PROTEIN 1 3424 8432 13458 4.8 3.9E+00 X64518.1 NT N. tabacum chitinase gene 50 for class I chitinase C 4194 9187 0. 73. 9E+00 AF055466. 1 NT Mus musculus seminal vesicle secretory protein 99 (MSVSP99) gene, promoter region 2557 7522 1. 46 3. 8E+00 AE001562. 1 NT Helicobacter pylori, strain J99 section 123 of 132 of the complete genome 3912 8912 13907 11. 92 3. 7E+00 AL161539. 2 NT Arabidopsis ianaDNAchromosome4, contigfragmentNo. 39 5050 10021 1. 53. 7E+00 AF216290. 1 NT Mus musculus heterochromatin protein 1 alpha mRNA, complete cds 588 5619 10617 3. 88 3. 6E+00 AV761055. 1 EST_HUMAN AV761055 MDS Homo sapiens cDNA clone MDSBUE10 5' 3174 8190 13211 0. 95 3. 5E+00 AF221538. 1 NT Cryptosporidium feDs heat shock protein 70 (HSP70) gene, partial cds 1481 6478 11534 2. 26 3.4E+00 AF254577. 1 NT Brassica napus RPB5d mRNA, complete cds 497 5533 10540 1. 62 3.2E+00 X96422. 1 NT D. rerio zp-50 POU gene Homo sapiens carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) (CEACAI 4588 9576 14567 1.44 3.2E+00 4502404 NT mRNA 2764 7785 12809 2.05 3.0E+00 8923984 NT Homo sapiens hypothetical protein PR00889 (PR00889), mRNA 19616947 12049 1. 6 2. 9E+00 AE002225. 2 NT Chlamydophila pneumoniae AR39, section 53 of 94 of the complete genome 1430 6427 11485 4.89 2.8E+00 AF186398.1 NT Buxus harlandii maturase K (matK) gene, partial cds ; chloroplast gene for chloroplast product 1593 6589 1. 65 2. BE+00 AL161552. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 52 230 5293 10301 19.61 2.7E+00 6679306 NT Mus musculus per-hexamer repeat gene 3 (Phxr3), mRNA 230 5293 10302 19. 61 2. 7E+00 6679306 NT Mus musculus per-hexamer repeat gene 3 (Phxr3), mRNA 4538 9528 14514 5. 78 2. 6E+00 AF068749. 1 NT Mus musculus sphingosine kinase (SPHK1b) mRNA, complete cds 1436 6433 11489 2. 05 2. 5E+00 AJ271844. 1 NT Aspergillus nidulans recQ gene for DNA helicase, exons 1-4 1436 6433 11490 2. 05 2.5E+00 AJ271844.1 NT Aspergillus nidulans recQ gene for DNA helicase, exons 1-4 2944 7963 12983 0.8 2.4E+00 M24282. 1 NT Chicken alpha-3 collagen type Vi mRNA, 3'end 4752 9737 1472s 5. 52 2. 4E+00 4503352 NT Homo sapiens double C2-like domains, alpha (DOC2A) mRNA 1234 6232 11276 13. 73 2. 3E+00 Z46724. 1 NT G. domesticus artificial single chain antibody gene (L3) 4002 8998 1. 54 2. 3E+00 AJ401081. 1 NT Bos taurus partial cytb gene for cytochrome b 3910 8910 13906 1.11 2.2E+00 AF020528. 1 NT Magnaporthe grisea Class IV chitin synthase (chs4) gene, complete cds 4186 9179 14162 4. 09 2.2E+00 D67071. 1 NT Rat gene for regucalcin, exon1 (non-coding exon) 4186 9179 14163 4.09 2.2E+00 D67071.1 NT Rat gene for regucalcin, exon1 (non-coding exon) 564 7724 10597 7. 72 2.1E+00 AF132612.2 NT Mus musculus pre-T cell receptor alpha gene, enhancer region and upstream region Page 3 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similer ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID Dalabase Top Hit Descrlptor ID NO: Signal BLAST E No. NO: NO: Source Value 2948 797 12985 1.2 2.1E+00 AF208432.1 NT Homo saplens falty acid omega-hydroxylase CYP4A11 (CYP4A11) gene, complete cds 3507 8515 0. 83 2. 1E+00 AW449366.1 EST HUMAN UI-H-B13-ald-e-08-0-UI.s1 NCl CGAP Sub5 Homo sapiens cDNA clone IMAGE : 2734550 3' 1177 6179 11215 2. 45 2. 0E+00 AF180527. 1 NT Homo sapiens p22Dokdel (DOKDEL) mRNA, complete cds 1177 6179 11216 2. 45 2. OE+00 AF180527. 1 NT Homo sapiens p22Dokdel (DOKDEL) mRNA, complete cds 1315 6312 11361 1. 07 2. 0E+00 AF204927.1 NT Orycloagus cuniculus Na+, K+-ATPase beta 1 subunit mRNA, complete cds 1540 6538 3.99 2.0E+00 P25582 SWISSPROT PUTATIVE RRNA METHYLTRANSFERASE SPB1 2088 7069 12182 8. 21 2. 0E+00 Z78279. 1 NT R. norvegicus mRNA for collagen alpha1 type I 2088 7069 12183 8. 21 2. 0E+00 Z78279. 1 NT R. norvegicus mRNA for collagen alphas type I hir13c05. x1 NCl_CGAP_GU1 Homo sapiens cDNA clone IMAGE : 2972168 3'similar to gb : X01677 3984 8982 13967 1.95 2. 0E+00 AW664496. 1 EST HUMAN GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, LtVER (HUMAN) ; hi13c05. x1 NCl_CGAP_GU1 Homo sapiens cDNA clone IMAGE : 2972168 3'similar to gb : X01677 3984 8982 13968 1, 95 2. 0E+00 W664496. 1 EST HUMAN GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, LIVER (HUMAN) 3019 8036 13046 1. 75 1. 8E+00 P21004 SWISSPROT PROTEIN B8 PRECURSOR Synechococcus sp. PCC7942 copper transporting P-ATPase (ctaA) and ATP synthase epsilon subunit 3047 8064 13072 2. 44 1. 8E+00 U04356. 1 NT (atpE) genes, complete cds Synechococcus sp. PCC7942 copper transporting P-ATPase (ctaA) and ATP synthase epsilon subunit 3047 8064 13073 2.44 1. 8E+00 U04356. 1 NT (atpE) agenes, complete cds LEVANSUCRASE (BETA-D-FRUCTOFURANOSYL TRANSFERASE) (SUCROSE 6-FRUCTOSYL 1091 6098 11127 1.65 1. 7E+00 Q60114 SWISSPROT TRANSFERASE) 2209 7186 12308 2. 62 1. 7E+00 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21C080 2312 7287 12408 1. 36 1.7E+00 Al141067.1 EST_HUMAN oz43h05.x1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE : 1678137 3' 1983 6968 12074 8. 04 1. 6E+00 AF199339. 1 NT Homo sapiens lens epithelium-derived growth factor gene, alternatively spliced, complete cds 1992 6977 12081 2. 96 1. 6E+00 AF077374. 1 NT Homo sapiens small proline-rich protein (SPRR3) gene, exons 1, 2, and 3 and complete cds 1996 6980 12085 1. 09 1. 6E+00 Y11344. 1 NT Mus musculus ST6GaINAcill gene, exon 2 2220 7197 4. 52 1. 6E+00 X98373.1 NT B. napus gene encoding endo-polygalacturonase zd25f01. r1 SoaresfetaLheartNbHH19W Homo sapiens cDNA clone IMAGE : 341689 5'similar to 2891 7910 12931 1. 57 1. 6E+o W58426. 1 EST HUMAN gb : D29805 N-ACETYLLACTOSAMINE SYNTHASE (HUMAN) ; 3921 8921 5. 49 1. 6E+00 BF570077. 1 EST_HUMAN 602186095T1 NIH_MGC_45 Homo sapiens cDNA clone IMAGE : 4310591 3' 4226 9220 14199 1. 68 1. 6E+00 AF155827. 1 NT Homo sapiens proliferation-associated SNF2-like protein (SMARCA6) mRNA, complete cds 4226 9220 14200 1. 68 1. 6E+00 AF155827. 1 NT Homo sapiens proliferation-associated SNF2-like protein (SMARCA6) mRNA, complete cds 4911 9890 14863 2. 77 1. 6E+00 Y11344. 1 NT Mus musculus ST6GalNAclll gene, exon 2 4911 9890 14864 2.77 1.6E+00 Y11344.1 NT Mus musculus ST6GalNAclll gene, exon 2 33 5113 10099 4. 61 1. 5E+00 U53449. 1 NT Ratlus norveglcus jun dimerization protein 2 ad-2) mRNA, complete cds 2315294 10303 1. 98 1. 5E+OOAE002201. 2 NT Chlamydophila pneumonlae AR39, section 32 of 94 of the complete genome Page 4 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Probe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID Dalabase Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value 616 5643 1.7 1.5E+00 6752961 NT Mus musculus a disintegrin and metalloproteinsse domain (ADAM) 15 (metargidin) (Adam15), mRNA 2344 7318 12438 1. 68 1. 5E+00 AJ131402. 1 NT Potato virus A RNA complete genome, isolate U 2444 7414 12530 1. 67 1. 5E+00 6678350 NT Mus musculus T-cell lymphoma lnvasion and metastasis 1 (Tiam1), mRNA 3065 7318 12438 2. 59 1. 5E+00 AJ131402. 1 NT Potato virus A RNA complete genome, isolate U 30 5110 10095 1. 17 1. 4E+00 7661685 NT Homo sapiens DKFZP586M0122 protein (DKFZP586M0122), mRNA 30 5110 10096 1. 17 1. 4E+00 7661685 NT Homo sapiens DKFZP586M0122 protein (DKFZP586M0122), mRNA 2272 7248 7. 96 1. 4E+00 U67922. 1 NT Ovis aries prion protein gene, complete cds 2598 7560 12677 1. 44 1. 4E+00 X74463. 1 NT Human papillomavirus type 7 genomic DNA Fugu rubripes neurofibromatosis type 1 (NF1), A-kinase anchor protein (AKAP84), BAW protein (BAW), ana 2701 7658 12771 3. 21 1. 4E+00 AF064564. 2 NT WSB1 protein (WSB1) genes, complete cds Fugu rubripes neurofibromatosis type 1 (NF1), A-kinase anchor protein (AKAP84), BAW protein (BAW), anc 2701 7658 12772 3. 21 1. 4E+00 AF064564. 2 NT WSB1 protein (WSB1) genes, complete cds 3259 8272 0. 89 1. 4E+00 5453733 NT Homo sapiens Mad4 homolog (MAD4) mRNA 4453 9443 1. 34 1. 4E+00 BF681547. 1 EST_HUMAN 602156687F1 NIH_MGC_83 Homo sapiens cDNA clone IMAGE : 4297556 5' 4994 9966 14944 1. 02 1. 4E+00 BE972426. 1 EST_HUMAN 601652250F1 NIH_MGC_82 Homo sapiens cDNA clone IMAGE : 3935556 5' 565 5598 1. 44 1. 3E+00 Z73640. 1 NT M. mucedo gene encoding 4-Dihydromethyl-trisporate dehydrogenase 891 5909 10949 2. 13 1. 3E+00 AJ271192. 1 NT Cantharellus sp. partial 25S rRNA gene, isolate Tibet 1112 6118 19. 14 1. 3E+00 Y19213. 1 NT Homo sapiens putative psihHbA pseudogene for hair keratin, exons 2 to 7 1278 6277 11318 14.53 1. 3E+00 4507998 NT Homo sapiens zinc finger protein 157 (HZF22) (ZNF157) mRNA 1278 6277 11319 14. 53 1. 3E+00 4507998 NT Homo sapiens zinc finger protein 157 (HZF22) (ZNF157) mRNA 1338 6336 1. 06 1. 3E+00 U61730.2 NT Coix lacryma-jobi dihydrodipicolinate synthase (dapA) gene, complete cds 1574 6571 2. 03 1. 3E+00 AE002338. 2 NT Chlamydia muridarum, section 66 of 85 of the complete genome 2479 7448 1. 67 BE966735. 3E+00 EST_HUMAN 601661233R1 NIH_MGC_72 Homo EST-HE-MAN cDNA clone IMAGE:3915945 3' Fugu rubripes gamma-aminobutyric acid receptor beta subunit gene, partial cds ; 55kd erythrocyte membrane protein (P55), synaptic vesicle-associated integral membrane protein (VAMP-1), procollagen C-proteinase 3517 8525 13536 0. 68 1. 3E+00 AF016494. 1 NT enhancer protein (PCOLCE) genes, complete c> 642 5670 10674 8. 78 1. 2E+00 AA676246. 1 SET_HUMAN zi22d08.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 431535 3' 813 5834 10867 0. 86 1. 2E+00 P05228 SWISSPROT HISTIDINE-RICH PROTEIN PRECURSOR (CLONE PFHRP-III) 813 5834 10868 0. 86 1. 2E+00 P05228 SWISSPROT HISTIDINE-RICH PROTEIN PRECURSOR (CLONE PFHRP-III) 813 5834 10869 0. 86 1. 2E+00 P05228 SWISSPROT HISTIDINE-RICH PROTEIN PRECURSOR (CLONE PFHRP-III) 867 5885 1. 83 1. 2E+00 8924234 NT Homo sapiens hypothetical protein PR03077 (PR03077), mRNA 1143 6147 11179 6. 07 1. 2E+00 AF080245. 2 NT Elaeis oleifera sesquiterpene synthase mRNA, complete cds 11866187 11224 1. 7 1. 2E+00 AJ252242. 1 NT pea seed-borne mosaic virus complete genome Page 5 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databsse Top Hit Descriptor ID NO: Signal BLAST E No. NO : NO Value Source 11866187112251. 71. 2E+00 AJ252242. 1 NT pea seed-borne mosaic virus complete genome 1960 6946 12048 0. 97 1. 2E+00 AF140631. 1 NT Homo sapiens G-protein coupled receptor 14 (GPR14) gene, complete cds 3092 8108 13124 6. 13 1. 2E+00 AL161563. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 63 3092 8108 13125 6.13 1.2E+00 AL161563. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 63 3213 8228 3.29 1. 2E+00 P54910 SW ISSPROT CONJUGAL TRANSFER PROTEIN TRBE PRECURSOR 3277 8289 13314 0. 73 1. 2E+00 AF188740. 1 NT Homo sapiens LHX3 gene, intron 2 3630 8636 13641 8. 37 1.2E+00 U75902. 1 NT Mus musculus subtilisin-like serine protease LPC (PC7) gene, exons 1 to 9, partial cds 3880 8881 13884 2.04 1. 2E+00 BF373570. 1 ESTHUMAN MRO-FT0175-050900-203-g061FT0175 Homo sapiens cDNA 4174 8289 13314 0. 97 1. 2E+00 AF188740. 1 NT Homo sapiens LHX3 gene, intron 2 4273 9266 14257 1. 01 1. 2E+00 6980951 NT Rattus norvegicus Glycine receptor alpha 2 subunit (glycine receptor, neonatal) (Glra2), mRNA 4346 9337 2.1 1. 2E+00 M87060. 1 NT Rattus rattus cardiac AE3 gene, exons 1-23 4392 9383 14365 0. 99 1. 2E+00 AL161509. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 21 4429 9419 14405 1. 92 1. 2E+00 AF156495.1 NT Homo sapiens post-synaptic density 95 (DLG4) gene, complete cds 4456 9446 5. 44 1. 2E+00 Y09200. 1 NT T. pinnatum chloroplast rbcl. gene, partial 461 5498 10509 1 1.1E+00 D86980. 1 NT Human mRNA for KIAA0227 gene, partial cds 1725 6720 11798 1. 36 1. 1E+00 AW995393. 1 EST HUMAN QVO-BN0042-170300-163-g12 BN0042 Homo sapiens cDNA 3255 8268 13290 7. 24 1. lE+00 AL163213. 2 NT Homo sapiens chromosome 21 segment HS21C013 3255 8268 13291 7. 241. 1E+00 AL163213. 2NT Homo sapiens chromosome 21 segment HS21C013 3406 8415 13443 0. 72 1. 1E+00 8922641 NT Homo sapiens hypothetical protein FLJ10749 (FLJ10749), mRNA 3479 8487 2. 43 1. 1E+00 8922973 NT Homo sapiens hypothetical protein FLJ11280 (FLJ11280), mRNA wf54h11. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2359461 3'similar to 3499 8507 13522 0.93 1.1E+00 Al808360. 1 EST HUMAN SW : P531 HUMAN Q12888 P53-BINDING PROTEIN 53BP1 ; 3636 8642 13647 1. 321. 1E+00 AE003886. 1 NT Xylella fastidiosa, section 32 of 229 of the complete genome 3636 8642 13648 1.32 1.1E+00 AE003886. 1 NT Xylella fastidiosa, section 32 of 229 of the complete genome 3840 8842 13849 0. 95 1.1E+00 8922641 NT. Homo sapiens hypothetical protein FLJ10749 (FLJ10749), mRNA 3913 8913 13908 0. 88 1. 1E+00 6755205 NT Mus musculus proteasome (prosome, macropain) subunit, beta type 7 (Psmb7), mRNA 4094 9088 8.1 1.1E+00 5835331 NT R. unicornis complete mitochondrial genome 4834 9818 14796 4. 26 1.1E+00 U18466. 1 NT African swine fever virus, complete genome 4899 9878 14847 1. 11 1. 1E+00 X78425. 1 NT E. faecalis pbp5 gene 5024 9995 14968 0.71 1.1E+00 P25396 sWISSPROT TELLURITE RESISTANCE PROTEIN TEHA 5058 10028 14996 0. 97 1.1E+00 AJ251835. 1 NT Mus musculus Kcnq1, Ltrpc5, Mash2, Tapa-1, Tssc4 and Tssc6 genes, alternative transcripts 98 5175 3.83 1.0E+00 U23808. 1 NT Xenopus laevis rhodopsin gene, complete cds 113 5185 10194 2. 17 1. 0E+00 D88425. 1 NT Cavia cobaya mRNA for serine/threoine kinase, complete cds 415 5452 2.22 1.0E+00 AB021684. 1 NT Marchantia polymorpha genes for 26S rRNA, 5S rRNA, 18S rRNA, 5. 8S rRNA and 26S rRNA Page 6 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database ID NO: Signal BLAST E No Top Hit Descriptor NO : NO : Value Source 571 5604 10602 1.57 1.0E+00 AJ251660. 1 NT Girardia tigrina mRNA for homeodomain transcription factor (so gene) 6695694 107039. 11. 0E+00 AL163218. 2 NT Homo sapiens chromosome 21 segment HS21C018 670 5695 0. 89 1. OE+00 AF125984. 1 NT Aedes aegypti mucin-like protein MUC1 mRNA, complete cds 1365 7746 3. 02 1. OE+00 X80416. 1 NT V. carteri Algal-CAM mRNA 1719 6714 11791 1.39 1.0E+00 AB006531. 1 NT Plautia stali intestine virus RNA for nonstructural polyprotein, capsid protein precursor, complete cds 2414 7385 12504 1.05 1.0E+00 P48355 SWISSPROT DNA GYRASE SUBUNIT B 2414 7385 12505 1.05 1.0E+00 P48355 SWISSPROT DNA GYRASE SUBUNIT 8 2806 7826 12841 3. 99 1.0E+00 P24008 SWISSPROT 3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 1 (STEROID 5-ALPHA-REDUCTASE 1) (SR TYPE 1) 2806 7826 12842 3.99 1.0E+00 P24008 SWISSPROT 3-OXO-5-ALPHA-STEROID 4-DEHYDROGENASE 1 (STEROID 5-ALPHA-REDUCTASE 1) (SR TYPE 1) 2897 7916 0. 98 1. 0E+W 014226 SWISSPROT HYPOTHETICAL 67. 9 KD PROTEIN C6F12. 08C IN CHROMOSOME I af26g08. s1 SoarestotaLfetusNb2HF89w Homo sapiens cDNA clone IMAGE : 1032830 3'similar to 3126 8142 13163 0. 82 1. OE+00 AA628453. 1 EST HUMAN WP : C42D8. 3 CE04204: contains element MER22 MER22 repetitive element ; 3519 5175 0. 92 1. OE+00 U23808. 1 NT Xenopus laevis rhodopsin gene, complete cds 3597 8604 13612 1. 71 1. OE+0 AJ223816. 1 NT Agaricus bisporus mRNA for tyrosinase Homo sapiens calcium channel alpha1 E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively 3956 8954 13943 1 1.0E+00 AF223391. 1 NT spliced 4153 9148 1. 071. 0E+00 8922245 NT Homo sapiens hypothetical protein FLJ10139 (FLJ10139), mRNA 4660 9645 14633 1. 73 1. OE+00 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21C047 4751 9736 1.35 1.0E+00 U75741. 1 NT Taenia ovis 45W antigen (ToW4) gene, complete cds 4859 9840 0. 93 1. OE+00 D10852. 1 NT Rattus norvegicus mRNA for N-acetylglucosaminyltransferase III, complete cds 4978 9954 1. 12 1. OE+00 AF200817. 1 NT Pilot whale morbillivirus phosphoprotein (P) gene, partial cds 5031 10002 1. 36 1. OE+00 AJ245481. 2 NT Human immunodeficiency virus type 1 proviral complete genome, isolate 95ML84 2566 7529 12647 0. 95 9. 9E-01 AL163302. 2 NT Homo sapiens chromosome 21 segment HS21C102 3524 8531 0. 82 9. 9E-01 AF174585. 1 NT Apple mosaic virus RNA 2 putative polymerase gene, complete cds 519 5554 10557 0. 97 9. 8E-01 P22567 SW ISSPROT AMINO-ACID ACETYLTRANSFERASE (N-ACETYLGLUTAMATE SYNTHASE) (AGS) (NAGS) 2729 7686 0.98 9.8E-01 AF174644.1 NT Xenopus aevis rac GTPase mRNA, complete cds 4315 9307 14291 0.68 9. 6E-01 AF197925. 1 NT Bromus inermis putative cytosofic phosphogfucomutase (pgm1) mRNA, complete cds 4315 9307 14292 0.68 9.6E-01 AF197925. 1 NT Bromus inermis putative cytosolic phosphoglucomutase (pgm1) mRNA, complete cds 4336 9327 14312 1.34 9.6E-01 AW799674.1 EST_HUMAN PM2-UM0053-240300-005-f12 UM0053 Homo sapiens cDNA 2404 7375 12495 1. 44 9. 5E-01 7705591 NT Homo sapiens CGI-125 protein (LOC51003), mRNA 3694 8698 13699 2.08 9.5E-01 BE902340. 1 EST_HUMAN 601675639F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3958473 5' Page 7 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar 0 ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID Database ID NO: Signal BLAST E No Top Hit Descriptor NO: NO: Source Value 3694 8698 13700 2. 08 9.5E-01 BE902340. 1 EST_HUMAN 601675639F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE:3958473 5' 3128 8144 3. 87 9. 4E-01 AF165990. 1 NT Bartonella clarridgeiae RNA polymerase beta subunit (rpoB) gene, partial cds 3146 8162 1. 76 9. 4E-01 AF080595. 1 NT Pimpinella brachycarpa zinc finger protein (ZFP1) mRNA, complete cds 1694 6689 1. 02 9. 3E-01 AF242382. 1 NT Homo sapiens phytanoyl-CoA hydroxylase (PHYH) gene, exon 5 2562 7526 12644 2. EST_HUMAN RC5-BT0503-271199-011-B01 BT0503 Homo EST-HUMAN cDNA 3166 8182 13205 3.17 9. 2E-01 BE622702. 1 EST HUMAN 601441338T1 NIH MGC 72 Homo sapiens cDNA clone IMAGE : 3916184 3' 2065 7047 2.24 9.1E-01 8923056 NT Homo sapiens hypothetical protein FLJ20048 (FLJ20048), mRNA 3132 8148 13168 0 83 9. 1E-0 T26418. 1 EST HUMAN AB200G8R Infant brain, LLNL array of Dr. M. Soares 1NIB Homo sapiens cDNA clone LLAB200G8 5' 3132 8148 13169 0.83 9.1E-01 T26418.1 EST_HUMAN AB200G8R Infant brain, LLNL array of Dr. M. Scares 1NIB HomosapienscDNA cloneLLAB200G8 5' 4254 9248 14232 1.01 9.0E-01 AF099810. 1 NT Homo sapiens neurexin III-alpha gene, partial cds 4411 9401 14386 2.16 8.8E-01 026350 SWISSPROT PUTATIVE F420-DEPENDENT NADP REDUCTASE 462 5499 10510 1. 83 8. 7E-01 AF106953. 2 NT Homo sapiens SOS1 (SOS1) gene, partial cds 2804 7824 12839 5.88 8.7E-01 AA595863. 1 EST_HUMAN nn05f11.s1 NCl_CGAP_Pr4. 1 Homo sapiens cDNA clone IMAGE : 1076877 Pseudomonas aeruginosa topoisomerase (top), putative transcriptional regulatory protein OhbR (ohbR). ortho- halobenzoate 1, 2-dioxygenase beta-ISP protein OhbA (ohbA), OhbC (ohbC), ortho-halobenzoate 1, 2- 4847982948. 7E-01 AF121970. 1 NT dioxygenase alpha-ISP protein OhbB (ohbB), and put> 471 5507 1.63 8.6E-01 X17012.1 NT Rat IGFII gene for insulin-like growth factor 11 848 5867 10908 8.21 8.6E-01 W69089. 1 EST HUMAN zd44eO3. r1 Soares fetal heart NbHH19W Homo sapiens cDNA clone IMAGE : 343516 5' 3539 8545 13552 0. 72 8. 6E-01 AL161565. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 65 3710 8714 13716 1. 03 8. 6E-01 U49724. 1 NT Drosophila melanogaster merlin (Dmerlin) mRNA, complete cds 732 5755 10776 2.07 8.3E-01 M93437. 1 NT Thermus thermophilus cytochrome c-552 (cycA) and CycB (cycB) genes, complete cds 3021 8038 13047 3. 04 8. 3E-01 AL161506. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 18 3905 8905 13903 2. 61 8.3E-01 Y19177. 1 NT Streptomyces antibioticus polyketide biosynthetic gene cluster 1999 6982 12086 1.66 8. 2E-01 AB000489. 1 NT Rattus norvegicus mRNA for RPHO-1, complete cds 2608 7570 1.22 8.2E-01 AW376990. 1 EST_HUMAN IL3-CT0219-161199-031-C08 CT0219 Homo sapiens cDNA 3819 8821 13828 0. 93 8. 2E-01 AF063417. 1 NT Tanystylum orbiculare elongation factor 1-alpha mRNA, partial cds 4935 9912 14890 0.99 8. 2E-01 AB000489. 1 NT Rattus norvegicus mRNA for RPHO-1, complete cds 2684 7642 0.93 8.1E-01 AF191839. 1 NT Mus musculus TANK binding Kinase TBK1 (Tbk1) mRNA, complete cds 3376 8384 13404 2. 84 8.1E-01 AF055066. 1 NT Homo sapiens MHC class 1 region 3376 8384 13405 2. 84 8. 1E-01 AF055066. 1 NT Homo sapiens MHC class 1 region 176 5240 2.17 8.0E-01 AJ271510. 1 NT Staphylococcus aureus partial pta gene for phosphate actyitransferase allele 15 286 5344 10356 8. 83 8. 0E-01 AJ132772. 1 NT Bos taurus futb and rtlf genes Page 8 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database ID NO: Signal BLAST E No Top Hit Descriptor NO: NO: Source Value 1984 6969 1.42 8.0E-01 BF630962.1 EST_HUMAN 602072473F1 NCl_CGAP_Bm67 Homo sapiens cDNA clone IMAGE:4215091 5' 3003 8021 13034 1. 22 8. 0E-01 AF127897. 1 NT Saimiri boliviensis olfactory receptor (SB027) gene, partial cds 3239 8252 13273 1. 18 8. OE-01 AB006193. 1 NT Mus musculus gene for oviductal glycoprotein, complete cds 3621 8628 1. 19 8. 0E-01 AL162758. 2 NT Neisseria meningitidis serogroup A strain Z2491 complete genome ; segment 7/7 4402 9393 14377 7. 03 B. OE-01 X83739. 2 NT G. gallus mRNA for nicotinic acelylcholine receptor (nAChR) beta 3 subunit 4822 9806 14788 1. 12 8. 0E-01 7657352 NT Mus musculus myosin IXb (Myo9b), mRNA 451 5488 10503 1. 55 7. 9E-01 D11476. 1 NT Lymantria dispar nuclear polyhedrosis virus gene for DNA polymerase, complete cds 705 5729 0. 79 7. 9E-01 AE002130. 1 NT Ureaplasma urealyticum section 31 of 59 of the complete genome 1568 MM 14. 05 7. 9E-01 AB040885. 1 NT Homo sapiens mRNA for KIAA1452 protein, partial cds 2201 7179 12302 8. 75 7. 9E-01 AB004816. 1 NT Oryctolagus cuniculus mRNA for mitsugumin29, complete cds 2202 7180 12303 1.53 7.9E-01 AF130459.1 NT Danio rerio Trp4-associated protein Tap1A (tap1A) mRNA, complete cds 3438 8446 13472 2.93 7.9E-01 AF228664.1 NT Gallus gallus SOX8 transcription factor (SOX8) mRNA, complete cds 4176 9170 1 0. 7. 9E-01 BE263612, 1 EST HUMAN 601192033F1 NIH MGC 7 Homo sapiens cDNA clone IMAGE : 3535785 5' 4479 9469 14449 1. 35 7. 9E-01 6753745 NT Mus musculus embigin (Emb), mRNA 4479 9469 14450 1. 35 7. 9E-01 6753745 NT Mus musculus embigin (Emb). mRNA 865 5883 2. 29 7. 8E-01 Z43785. 1 EST HUMAN HSC1KH041 normalized infant brain cDNA Homo sapiens cDNA clone c-1kh04 2213 7190 12311 2. 82 7. 8E-01 AW959567. 1 EST HUMAN EST371637 MAGE resequences, MAGF Homo sapiens cDNA 4563 9551 14537 0. 79 7. 8E-01 U87305. 1 NT Rattus norvegicus transmembrane receptor Unc5H1 mRNA, complete cds 4871 9850 1. 51 7. 8E-01 AW753353. 1 EST HUMAN RC3-CT0254-130100-023-c02 CT0254 Homo sapiens cDNA 143 5209 10223 4. 43 7. 7E-01 AF184345. 1 NT Lycopersicon hirsutum ADP-glucose pyrophosphoryiase large subunit (AGP-L1) mRNA, complete cds Mus musculus major histocompatibility locus class 11 region : major histocompatibility protein class il alpha chain (alpha) and major histocompatibility protein class It beta chain (IEbeta) genes, complete cds ; 716 5739 1. 61 7. 7E-01 AF050157. 1 NT butyrophilin-like (NG9), butyrophilin-li> 2640 7600 12714 1.56 7. 7E-01 033915 SWISSPROT CITRATE SYNTHASE 3520 8527 13538 3. 71 7. 7E-01 AF118085. 1 NT Homo sapiens PRO1975 mRNA, complete cds 4272 9265 14255 3. 89 7. 7E-01 AF199488. 1 NT Coturnix coturnix japonica sub-species japonica beta-actin mRNA, partial cds 4272 9265 14256 3. 89 7. 7E-01 AF199488. 1 NT Coturnix coturnix japonica sub-species japonica beta-actin mRNA, partial cds 509 5544 1. 38 7. 5E-01 AL163301. 2 NT Homo sapiens chromosome 21 segment HS21C101 579 5611 10610 1 7. 5E-01 AF020503. 1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydrolase (FHIT) gene, exon 5 5070 10039 15006 0. 95 7. 5E-01 6981387 NT Rattus norvegicus Protein phosphatase 1, catalytic subunit, beta isoform (Ppplcb), mRNA tn14bO9 x1 NCl_CGAP_Brn25 Homo sapiens cDNA clone IMAGE : 2167577 3'similar to contains Alu 1113 6119 11147 1. 25 7. 4E-01A1598146. 1 EST HUMAN repetitive element ; contains element MIR repetitive element ; Page 9 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST. E No. Source Top Hit Descriptor Value 2281 7257 12375 0. 97 7. 4E-01 AB011106. 1 NT Homo sapiens mRNA for KIAA0534 protein, partial cds 3643 8649 13655 0. 87 7. 4E-01 AF112538.1 NT Malva pusilla actin (Act1) mRNA, complete cds 4184 9177 14161 8. 61 7. 4E-01 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21C046 xp83d04. x1 NCiCGAPOv40 Homo sapiens cDNA clone IMAGE : 2746951 3'similar to contains element 5013 9984 14960 1. 07 7. 4E-01 AW270642. 1 EST_HUMAN AER35 MER35 repetitive element ; 4483 9473 14453 0. 84 7. 3E-01 AE001166. 1 NT Borrelia burgdorferi (section 52 of 70) of the complete genome 4562 9550 14536 4. 94 7. 3E-01 AF225421. 1 NT Homo sapiens HT017 mRNA, complete cds 5046 10017 14986 0.92 7. 3E-01 AJ400861. 1 NT Cicer arietinum partial mRNA for putative UDP-glycose 821 5841 2. 09 7. 2E-01 L29281. 1 NT Rattus norvegicus initiation factor-2 kinase (elF-2a) mRNA, complete cds 1917 6903 11997 3. 43 7. 2E-01 X79140. 1 NT N. tabacum NeIF-4A13 mRNA 2392 7363 12485 1. 25 7. 2E-01 AB009605. 1 NT Gallus gallus gene for melanocortin 2-receptor, complete cds 2993 8011 13023 1. 4 7. 2E-01 AF198100. 1 NT Fowlpox virus, complete genome 3370 8378 13398 2. 78 7. 2E-01 AF065606. 1 NT Giardia intestinalis variant-specific surface protein (vsp417-6) gene, vsp417-6/A-I allele, complete cds 3533 8539 13545 0. 98 7. 2E-01 AB002307. 1 NT Human mRNA for KIAA0309 gene, partial cds 3780 8783 13787 2. 44 7.2E-01 BF338350.1 EST_HUMAN 602035589F1 NCl_CGAP_Brn64 Homo sapiens cDNA ozone IMAGE : 4183222 5' 4624 9609 14597 3.41 7.2E-01 D90314.1 NT Lmesenteroides gene for sucrose phosphorylase (EC 2.4.1.7) Homo sapiens transcription factor IGHM enhancer 3, JM11 protein, JM4 protein, JM5 protein, T54 protein, JM10 protein, A4 differentiation-dependent protein, triple LIM domain protein 6, and synaptophysin genes, 4949 9926 14904 1. 37 7. 2E-01 AF196779. 1 NT complete cds ; and L-type calcium channel a> Homo sapiens transcription factor GHM enhancer 3, JM11 protein, JM4 protein, JM5 protein, T54 protein, JM10 protein, A4 differentiation-dependent protein, triple LIM domain protein 6, and synaptophysin genes, 4949 9926 14905 1. 37 7. 2E-01 AF196779. 1 NT complete cds ; and L-type calcium channel a> NUCLEOSIDE TRIPHOSPHATASE I (NUCLEOSIDE TRIPHOSPHATE PHOSPHOHYDROLASE I) (NPI- 4975 9951 14929 0. 78 7. 2E-01 P33066 SW ISSPROT I) Rana catesbeiana mRNA for bullfrog skeletal muscle calcium release channel (ryanodine receptor) alpha 683 5707 10719 9. 06 7. 1 E-01 D21070. 1 NT isoform(RyR1), complete cds 2989 8007 13020 14. 39 7. 1E-01 AJ270777. 1 NT Homo sapiens partial TCF-4 gene for T-cell transcription factor-4, exons 15-16 4086 9080 14070 3. 49 7. 1E-01 7305360 NT Mus musculus otogelin (Otog), mRNA 4086 9080 14071 3. 49 7. 1E-01 7305360 NT Mus musculus otogelin (Otog), mRNA 1209 6209 11248 2. 58 7. 0E-01 AB014514. 1 NT Homo sapiens mRNA for KIAA0614 protein, partial cds 1209 6209 11249 2.58 7. 0E-01 AB014514. 1 NT Homo sapiens mRNA for KIAA0614 protein, partial cds yz73e07. s1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE : 288708 3'similar to 2381 7353 12474 1.03 7. 0E-01 N62412. 1 EST HUMAN contains Alu repetitive element ; Page 10 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO : Signal BLAST E No. Database Top Hit Descriptor NO: NO: Source Value yz73e07.s1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE:288708 3' slmilar to 2381 7353 12475 1. 03 7. 0E-01 N62412. 1 EST HUMAN contains Alu repetitive element ; 4889 9868 1. 81 7. 0E-01 AL163301. 2 NT Homo sapiens chromosome 21 segment HS21C101 Candida albicans squalene epoxidase (CAERG1) gene, complete cds and translational regulator gene, partial 955 5971 11004 15. 93 6. 9E-01 U69674. 1 NT cds Candida albicans squalene epoxidase (CAERG1) gene, complete cds and translational regulator gene, partial 955 5971 11005 15. 93 6. 9E-01 U69674. 1 NT cds 1291 6290 1133 1. 7 6. 9E-01 AA593530. 1 EST HUMAN nn28aO9. s1 NCI_CGAP_Gas1 Hom saplens cDNA clone IMAGE : 1085176 3' 3148 8164 13184 1. 56 6. 9E-01 AE002271. 2 NT Chlamydia muridarum, section 3 of 85 of the complete genome 942 5959 10992 2. 14 6. 8E-01 AF017784. 1 NT Giardia intestinalis carbamate kinase gene, complete cds 2603 7565 1. 2 6.8E-01 D90917. 1 NT SynechocysUs sp. PCC6803 complete genome, 27/27, 3418852-3573470 4439 9429 14413 1. 39 6. 8E-01 J00762. 1 NT Rat (hooded) prolactin gene : exon iii and flanks Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-celis 1 (NFKB1) gene, complete 296 5353 10366 24. 4 6. 7E-01 AF213884. 1 NT cds Homo sapiens nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (NFKB1) gene, complete 337 5389 10396 21. 87 6. 7E-01 AF213884. 1 NT cds zx12g12. s1 Soares_total_fetus Nb2HF8 9w Homo sapiens cDNA clone IMAGE : 786310 3'similar to 2086 7067 12179 1. 15 6. 7E-01 AA451864. 1 EST HUMAN contains element TAR1 repetitive element ; Drosophila melanogaster Mst85C gene, complete cds ; NMDMC isoform (Nmdmc) gene, complete cds, 2102 7763 12198 2. 56 6. 7E-01 AF186073. 1 NT alternatively spliced ; and transcription factor (Relish) gene, complete cds, alternatively spliced 2927 7946 12963 | 4 7E-01 6678580 NT Mus musculus Wiskott-Aldrich syndrome protein (Wasp), mRNA 4331 9322 14308 0. 7 6. 7E-01 X74421. 1 NT S. tuberosum mRNA for glucose-6-phosphate dehydrogenase 2628 7588 12700 1.28 6.6E-01 AF199339.1 NT Homo sapiens lens epithelium-derived growth factor gene, alternatively spliced, complete cds Homo sapiens sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain 3409 8418 13446 0. 93 6. 6E-01 4506880 NT (TM) and short cytoplasmic domain, (semaphorin) 5A (SEMA5A) mRNA 3574 8581 13587 3. 48 6. 6E-01 Y07669. 1 NT C. albicans random DNA marker, 282bp Human hereditary haemochromatosis region, histone 2A-like protein gene, hereditary haemochromatosis 3993 8990 1. 05 6. 6E-01 U91328. 1 NT (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds 618 5645 10647 1. 67 6. 5E-01 M75140. 1 NT H. vulgaris Na, K-ATPase alpha subunit mRNA, complete cds 618 5645 10648 1. 67 6. 5E-01 M75140. 1 NT H. vulgaris Na, K-ATPase alpha subunit mRNA, complete cds 3348 8357 13375 4. 84 6. 5E-01 AB041225. 1 NT Mus musculus gene for Tob2, complete cds 4159 9154 14137 5.18 6.5E-01 AJ272265.1 NT Homo sapiens SPP2 gene for secreted phosphoprotein 24 precursor, exons 1-8 Page 11 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database ID NO: Signal BLAST E No Top Hit Descriptor NO: NO: Source Value 4894 9873 14840 2.32 6.5E-01 U28921.1 NT Phaseolus vulgaris ATPase gamma subunit mRNA, nuclear gene encoding mitochondrial protein, partial cds 5073 10042 15008 1. 39 6. 5E-01 8924057 NT Homo sapiens hypothetical protein PRO1580 (PRO1580), mRNA 250 5310 10321 8. 85 6. 4E-01 U48848. 1 NT Drosophila melanogaster 8kd dynein light chain mRNA, complete cds 2511 7479 12594 0.9 6. 4E-01 AF161184. 1 NT Pseudomonas fluorescens tryptophan halogenase (prnA) gene, complete cds 3374 8382 13402 3. 74 6. 4E-01 U48854. 2 NT Mus musculus dystroglycan 1 (DAG1) gene, exons 1 and 2 and complete cds 3771 8774 13778 1.19 6. 4E-01 AB046827. 1 NT Homo sapiens mRNA for KIAA1607 protein, partial cds 4364 9355 14334 0. 74 6. 4E-01 Y12488. 1 NT M. musculus whn gene 4364 9355 14335 0. 74 6. 4E-01 Y12488. 1 NT M. musculus whn gene 431 5469 10488 3.16 6. 3E-01 P05228 SWISSPROT HISTIDINE-RICH PROTEIN PRECURSOR (CLONE PFHRP-III) 531 5566 10569 1. 73 6. 3E-01 U32689. 1 NT Haemophilus influenzae Rd section 4 of 163 of the complete genome 2098 7079 12193 25.01 6. 3E-01 U81136. 1 NT Shigella flexneri multi-antibiotic resistance locus 2503 7471 12587 1. 82 6. 3E-01 U75331. 1 NT Gallus gallus bone morphogenetic protein 1 (BMP1) mRNA, partial cds 2503 7471 12588 1. 82 6. 3E-01 U75331. 1 NT Gallus gallus bone morphogenetic protein 1 (BMP1) mRNA, partial cds 2949 7968 0. 67 6. 3E-01 Y17275. 1 NT Lycopersicon esculentum p69a gene, complete CDS 2330 7304 3.53 6.1E-01 6678076 NT Mus musculus secreted acidic cystine rich glycoprotein (Sparc), mRNA 4405 9396 14379 0. 98 6. 1 E-01 4557538 NT Homo sapiens solute carrier family 26 (sulfate transporter), member 2 (SLC26A2) mRNA 491 5527 10535 1. 08 6.0E-01 D87675. 1 NT Homo sapiens DNA for amytoid precursor protein, complete cds 557 5591 2. 76 6.0E-01 5802999 NT Homo sapiens adaptor-related protein complex 3, mu 2 subunit (CLA20), mRNA 1345 6342 11393 1. 38 6. OE-01 AF065253. 1 NT Human respiratory syncytial virus strain CH93-53b attachment protein (G) gene, complete cds 3725 8729 13727 0. 81 6. OE-01 AJ233396. 1 NT Viral hemorrhagic septicemia virus N, P, M, G, Nv, L genes, French strain 07-71 3923 8923 13914 2. 18 6. OE-01 X16842. 1 NT Xenopus mRNA for desmin 4064 9058 1. 03 6. OE-01 AF058895. 1 NT Homo sapiens Notch3 (NOTCH3) gene, exons 26, 27, and 28 985 6000 11031 1. 03 5. 9E-01 U32701. 1 NT Haemophilus influenzae Rd section 16 of 163 of the complete genome 3196 8212 13233 9.18 5. 9E-01 AL163267. 2 NT Homo sapiens chromosome 21 segment HS21 C067 3196 8212 13234 9. 18 5. 9E-01 AL163267. 2 NT Homo sapiens chromosome 21 segment HS21 C067 4100 9094 3 5.9E-01 AF162756. 1 NT Rattus norvegicus cenexin 2 mRNA, partial cds 1866 6855 11943 1. 05 5. 8E-01 P40472 ISSPROT SIM1 PROTEIN 3876 8877 13880 0. 97 5. 8E-01 BF695738. 1 EST HUMAN 601852474F1 NIH MGC 56 Homo sapiens cDNA clone IMAGE : 4076131 5' 4383 9374 14353 4. 15. 8E-01 AB009077. 1 NT Vigna radiata mRNA for proton pyrophosphatase, complete cds 4662 9637 1.31 5.8E-01 AF110846.1 NT Megaselia scalaris sex-lethal homolog (Megsxl) gene, partial cds, altematively spliced products 4775 9759 2.29 5.8E-01 AW769483. 1 EST HUMAN hl64f10.x1 NCl_CGAP_Kid13 Homo sapiens cDNA clone IMAGE : 3005995 3' 1463 6460 11518 1. 04 5. 7E-01 P06727 SWISSPROT APOLIPOPROTEIN A-IV PRECURSOR (APO-AIV) 1463 6460 11519 1.04 5.7E-01 P06727 SWISSPROT APOLIPOPROTEIN A-IV PRECURSOR (APO-AIV) Page 12 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Top Hit Descriptor DO: NO: Source Value 3152 8168 13188 1.38 5.7E-01 Q9WTJ2 SWISSPROT PUTATIVE TRANSCRIPTION FACTOR OVO-LIKE 1 (MOVO1) (MOVO1A) 3425 8433 2. 37 5. 7E-01 AB033503. 1 NT Populus euramericana peacs-2 mRNA for 1-aminocyclopropane-1-carboxyiate synthase, complete cds 5042 10013 14982 0. 95 5. 7E-01 L41867. 1 NT Drosophila extra sex combs gene, exon 1-4, complete cds 3283 8295 13320 1. 11 5. 6E-01 AB018283. 2 NT Homo sapiens mRNA for Kit0740 protein, partial cds 3283 8295 13321 1. 11 5.6E-01 AB018283. 2 NT Homo sapiens mRNA for KIAA0740 protein, partial cds 4117 9111 14095 2. 28 5. 6E-01 D83135. 1 NT Chicken TBP gene, exon8, complete cds 1192 6193 11230 1. 79 5. 5E-01 8393912 NT Rattus norvegicus Propionyl Coenzyme A carboxylase, beta polypeptide (Pccb), mRNA I GAG POLYPROTEIN [CONTAINS : INNER COAT PROTEIN P12 ; CORE PROTEIN P15 ; CORE SHELL 2629 7589 12701 2. 21 5. 5E-01 P03341 SWISSPROT PROTEIN P30 ; NUCLEOPROTEIN P10] GAG POLYPROTEIN [CONTAINS : INNER COAT PROTEIN P12 ; CORE PROTEIN P15 ; CORE SHELL 2629 7589 12702 2. 21 5. 5E-01 P03341 SWISSPROT PROTEIN P30 ; NUCLEOPROTEIN P10] 2848 7868 12884 0. 79 5. 5E-01 5902085 NT Homo sapiens superkiller viralicidic activity 2 (S. cerevisiae homolog)-like (SKIV2L), mRNA 2992 8010 1. 33 5. 5E-01 H46219. 1 EST HUMAN yo18a10. s1 Soares adult brain N2b5HB55Y Homo sapiens cDNA clone IMAGE : 178266 3' 3163 8179 13201 3. 6 5. 5E-01 AF227240. 1 NT Rabbit oral papillomavirus, complete genome 3608 8615 13624 1. 37 5. 5E-01 P48755 SW ISSPROT FOS-RELATED ANTIGEN-1 4982 9957 14935 0. 99 5. 5E-01 U69097. 1 NT Bos taurus MHC class 11 beta-chain BoLA-DIB1 gene, partial cds 144 5210 10224 13. 58 5. 4E-01 7657266 NT Homo sapiens KIAA0929 protein Msx2 interacting nuclear target (MINT) homolog (KIAA0929), m 144 5210 10225 13. 58 5. 4E-01 7657266 NT Homo sapiens KIAA0929 protein Msx2 interacting nuclear target (MINT) homolog (KIAA0929), mRNA Pseudomonas syringae pv. tomato strain DC3000 AvrE (avrE), HrpW (hrpW), and GstA (gstA) genes, 580 5612 10611 1. 77 5. 4E-01 AF232006. 1 NT complete cds ; and unknown genes Pseudomonas syringae pv. tomato strain DC3000 AvrE (avrE), HrpW (hrpW), and GstA (gstA) genes, 580 5612 10612 1. 77 5. 4E-01 AF232006. 1 NT complete cds ; and unknown genes 1252 6250 11291 2. 4 5. 4E-01 AW896087. 1 EST_HUMAN QV4-NNO040-070400-160-c04 NN0040 Homo sapiens cDNA 2048 7030 2. 35 5. 4E-01 AE002247. 2 NT Chlamydophila pneumoniae AR39, section 74 of 94 of the complete genome 2193 7172 12293 1. 53 5. 4E-01 AJ276682. 1 NT Drosop[hila melanogaster mRNA for 15,15' beta carotene dioxygenase (beta-diox gene) Homo sapiens HLA class III region containing tenascin X (tenascin-X) gene, partial cds ; cytochrome P450 21- hydroxyiase (CYP21 B), complement component C4 (C4B) G11, helicase (SKI2W), RD, complement factor B 512 5547 10552 1. 61 5. 3E-01 AF019413. 1 NT (Bf), and complement component C2 (C2) genes, > 2710 7667 12779 10. 07 5. 3E-01 4506328 NT Homo sapiens protein tyrosine phosphatase, receptor-type, zeta polypeptide 1 (PTPRZ1) mRNA 2710 7667 12780 10. 07 5. 3E-01 4506328 NT Homo sapiens protein tyrosine phosphatase, receptor-type, zeta polypeptide 1 (PTPRZ1) mRNA 3169 8185 13206 3. 67 5. 3E-01 AF087658. 1 NT Homo sapiens secreted C-type lectin precursor (LSLCL) gene, complete cds Page 13 of 209 Table 4 Single Exon Probes Expressed in HBL100 Probe Exon MOst Similar ORS SET Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descritpor NO: NO: Source Value 4090 9084 1.22 5.3E-01 U39687.1 NT Mycoplasma genitalium section 9 of 51 of the complete genome 806 5827 10857 12. 62 5. 2E-01 L20770. 1 NT Drosophila melanogaster helix-loop-helix mRNA, complete cds NUCLEAR FACTOR OF ACTIVATED T CELLS 5 (T CELL TRANSCRIPTION FACTOR NFAT5) (NF-AT5 1146 6150 11182 11. 32 5. 2E-01 Q9WV30 SWISSPROT (REL DOMAIN-CONTAINING TRANSCRIPTION FACTOR NFAT5) 1171 6174 11208 6. 03 5. 2E-01 AF224492. 1 NT Homo sapiens phospholipid scramblase 1 gene, complete cds 1847 6836 4. 17 5. 2E-01 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21C085 2085 7066 12178 1. 52 5. 2E-01 AB018283. 2 NT Homo sapiens mRNA for KIAA0740 protein, partial cds 3046 8063 13071 1. 72 5. 2E-01 U65942. 1 NT Chlamydophila abortus strain S26/3 POMP91A and POMP90A precursor, genes, complete cds 3321 8331 11. 56 5. 2E-01 AL116780. 1 NT Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation 3360 8368 13387 2. 52 5. 2E-01 AA984165. 1 EST HUMAN am77g05. s1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE : 1616504 3' Medicago sativa chloroplast malate dehydrogenase precursor (p1 mdh) mRNA, nuclear gene encoding 3547 8554 0. 79 5. 2E-01 AF020269. 1 NT chloroplast protein, complete cds 4358 9349 1. 46 5. 2E-01 AF093796. 1 NT Avian infectious bronchitis virus isolate variant 2 S1 spike glycoprotein gene, partial cds 4960 9937 1. 01 5. 2E-01 AL163281. 2 NT Homo sapiens chromosome 21 segment HS21C081 613 5640 10643 1. 5. 1E-01 M58509. 1 NT Human adrenodoxin reductase gene, exons 3 to 12 640 5668 10671 3. 68 5. 1E-01 AJ233944. 1 NT Polyangium vitellinum (strain PI vt1) 16S rRNA gene 640 5668 10672 3. 68 5. 1E-01 AJ233944. 1 NT Polyangium vitellinum (strain PI vt1) 16S rRNA gene 3963 8961 13951 4.37 5. 1E-01 AI858495.1 EST_HUMAN wl39b12.x1 NCl_CGAP_Ut1 Homo sapiens cDNA clone IMAGE : 2427263 3' 4062 9056 14043 2. 47 5. 1E-01 P96380 SWISSPROT TRANSCRIPTION-REPAIR COUPLING FACTOR (TRCF) 2076 7058 12167 1. 0E 5. 0E-01 4885552 NT Homo sapiens postmeiotic segregation increased 2-like 9 (PMS2L9), mRNA 2076 7058 12168 1. 03 5. 0E-01 4885552 NT Homo sapiens postmeiotic segregation increased 2-like 9 (PMS2L9), mRNA Buchnera aphidicola genomic fragment containing (chaperone Hsp60) groEL, DNA biosynthesis initiating protein (dnaA), ATP operon (atpCDGAHFEB), and putative chromosome replication protein (gidA) genes, 2083 7064 12174 1. 08 5. OE-01 AF008210. 1 NT complete cds ; and termination factor Rho (rho) gene> Buchnera aphidicola genomic fragment containing (chaperone Hsp60) groEL, DNA biosynthesis initiating protein (dnaA), ATP operon (atpCDGAHFEB), and putative chromosome replication protein (gidA) genes, 2083 7064 12175 1. 08 5. 0E-01 AF008210. 1 NT complete cds ; and termination factor Rho (rho) gene> 3740 8744 13744 1. 11 5. OE-01 L38483. 1 NT Rattus norvegicus jagged protein mRNA, complete cds 3782 8785 13788 2. 97 5. 0E-01 AB033010. 1 NT Homo sapiens mRNA for KIAA1184 protein, partial cds 781 5802 10832 2. 6 4. 9E-0 BF571462. 1 EST_HUMAN 602076649F1 NIH_MGC_62 Homo sapiens cDNA clone IMAGE : 4243860 5' 1619 6616 11682 4.93 4.9E-01 AJ243955. 1 NT Xenopus laevis mRNA for c-Jun protein, 1978 BP 1864 6853 11941 1. 4. 9E-01 U40869. 1 NT Cavia porcellus pulmonary surfactant protein A (SP-a) mRNA, complete cds 3460 8468 1.14 4. 8E-01 AA912842. 1 EST_HUMAN ol32a09.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 1525144 3' 3658 8664 13669 1.62 4. 6E-01 BF693300. 1 EST_HUMAN 602081103F1 NIH_MGC_81 Homo sapiens cDNA clone IMAGE : 4245481 5' Page 14 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hil SEQ ID SEQ ID ID NO : Signal BLAST E No. Database Top Hit Descriptor Value 3658 8664 13670 1. 62 4. 6E-01 BF693300. 1 EST_HUMAN 602081103F1 NIH_MGC_81 Homo sapiens cDNA clone IMAGE : 4245481 5' 2799 7819 128364. 48 4. 5E-01 AA677086. 1 EST_HUMAN zi55d02.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 454179 3' BASEMENT MEMBRANE-SPECIFIC HEPARAN SULFATE PROTEOGLYCAN CORE PROTEIN 3243 8256 13277 4. 25 4. 5E-01 Q05793 SWISSPROT PRECURSOR (HSPG) (PERLECAN) (PLC) 3293 8304 13329 1. 39 4.5E-01 AF1276378.1 NT Mus musculus DNA polymerase epsilon catalytic subunit (Pole) gene, exons 2 through 12 3917 8917 1.06 4.5E-01 Q28247 SWISSPROT COLLAGEN ALPHA 5 (IV) CHAIN 4052 10058 5.38 4. 5E-01 AW873495. 1 EST_HUMAN ho60g02.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 3041610 3' 4786 9770 14754 1. 4. 5E-01 BE963445. 2 EST_HUMAN 601657225R1 NIH_MGC_67 Homo sapiens cDNA clone IMAGE : 3866023 3' 1985 6970 2. 19 4. 4E-01 6680503 NT Mus musculus integral membrane-associated protein 1 (Itmap1), mRNA VASCULAR ENDOTHELIAL GROWTH FACTOR B PRECURSOR (VEGF-B) (VEGF RELATED 2325 7299 12420 4. 73 4. 4E-01 P49765 SWISSPROT FACTOR) 3241 8254 13275 1. 19 4. 4E-01 AF058790. 1 NT Rattus norvegicus SynGAP-b mRNA, complete cds 3241 8254 13276 1. 19 4. 4E-01 AF05B790. 1 NT Rattus norvegicus SynGAP-b mRNA, complete cds 3245 8258 13279 1. 81 4. 4E-01 BF056726. 1 EST_HUMAN 7j91d02.y1 NCl_CGAP_Brn16 Homo sapiens cDNA clone IMAGE : 3393795 5' 4113 9107 1. 55 4. 4E-01 BE378707. 1 EST_HUMAN 601237139F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3609393 5' 4938 9915 14894 6. 65 4. 4E-01 U61154. 1 NT Buzura suppressaria nucleopolyhedrosisvirus ecdysteroid UDP-glucosyltransferase (egt) gene, complete cds 408 5445 10465 1. 95 4. 3E-01 AF155218.1 NT Callithrixjacchus MWILW opsin gene, upstream flanking region 408 5445 10466 1. 95 4. 3E-01 AF155218. 1 NT Callithrixiacchus MW/LW opsin gene, upstream flanking region 2801 7821 1. 34 4. 3E-01 AW935269. 1 EST HUMAN CM2-DT0003-010200-077-c01 DT0003 Homo sapiens cDNA 2986 8004 13016 0. 72 4. 3E-01 AW999477. 1 ESTHUMAN MRO-BN0070-270300-008-g04 BN0070 Homo sapiens cDNA 4028 9024 14011 1. 2 4. 3E-01 J00306. 1. NT Human somatostatin gene and flanks 4282 5445 10465 1. 07 4. 3E-01 AF155218. 1 NT Callithrixjacchus MW/LW opsin gene, upstream flanking region 4282 5445 10466 1. 07 4. 3E-01 AF155218. 1 NT Callithrix jacchus MW/LW opsin gene, upstream flanking region 4944 9921 1. 08 4. 3E-01 9635250 NT Xestia c-nigrum granulovirus, complete genome 1341 7745 11389 1. 24 4. 2E-01 Q39102 SWISSPROT CELL DIVISION PROTEIN FTSH HOMOLOG PRECURSOR 3528 8534 13541 3. 83 4. 2E-01 AE003947. 1 NT Xylella fastidiosa, section 93 of 229 of the complete genome 3552 8559 13565 2. 9 4. 2E-01 AI280338.1 EST_HUMAN ql94b01.x1 Soares NhHMPu_S1 Homo sapiens cDNA clone IMAGE : 1879945 3' 3629 10057 0. 86 4. 2E-01 N81203. 1 EST HUMAN 788iE1 fetal brain cDNA Homo sapiens cDNA clone 788iE1-K similar to R07879, Z40498 nj69hO1. s1 NCl CGAP Pr10 Homo sapiens cDNA clone IMAGE : 997777 similar to gb : M33600 HLA CLASS 4558 9546 14531 5. 45 4. 2E-01 AA534093. 1 EST HUMAN 11 HISTOCOMPATIBILITY ANTIGEN DR-1 BETA CHAIN (HUMAN) ; 4636 9621 14613 3. 8 4. 2E-01 R13467. 1ESTHUMAN yf77e01. r1 Scares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 28278 5' 1077 6084 11113 1. 52 4. 1E-01 AI905481. 1 EST HUMAN RC-BT091-210199-142 BT091 Homo sapiens cDNA 10866093111222. 09 4. 1E-01 AV705243. 1 EST HUMAN AV705243 ADB Homo sapiens cDNA clone ADBAHF08 5' Page 15 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit A@@@@@@@ Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: NO: Dource Value 1086 6093 11123 2.09 4.1E-01 AV705243.1 EST_HUMAN AV705243 ADB Homo sapiens cDNA clone ADBAHF08 5' 1573 6570 11632 0.94 4.1E-01 AI905949. 1 EST HUMAN PM-BT103-270499-684 BT103 Homo sapiens cDNA 2638 7598 12712 1.1 4.1E-01 7705283 NT Homo sapiens anaphase-promoting complex subunit 7 (APC7), mRNA 2869 7888 12908 2. 49 4.1E-01 AL161536. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 36 2869 7888 12909 2.49 4.1E-01 AL161536. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 36 3229 8244 13266 0. 74. 1E-01 AA906344. 1 EST_HUMAN oj94b08.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 1505943 3' 4152 9147 14131 2. 75 4.1E-01 AJ249207. 1 NT Rhodococcus sp. AD45 isoG, isoH, isol, isoJ, isoA, isoB, isoC, isoD, isoE and isoF genes 4178 9172 0. 74 4.1E-01 AA909257. 1 ESTHUMAN om33d02. s1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 1542819 3' 4532 9522 14509 1. 37 4. 1E-01 AV747880. 1 EST HUMAN AV747880 NPC Homo sapiens cDNA clone NPCBDF10 5' 10226031 11061 0. 81 4. OE-01 8404656 NT Laqueus rubellus mitochondrion, complete genome 1319 6317 11365 1.16 4.0E-01 AF203478.1 NT Drosophila melanogaster Dalmatian (dmt) mRNA, complete cds 1454 6451 3. 09 4.0E-01 6679258 NT Mus musculus platelet derived growth factor receptor, beta polypeptide (Pdgfrb), mRNA 2733 5207 10222 2. 12 4.0E-01 6678490 NT Mus musculus ubiquitin-protein ligase e3 componen n-recognin (Ubr1), mRNA 2898 7917 12937 1. 16 4.0E-01 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21C080 2898 7917 12938 1. 16 4. OE-01 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21C080 Streptococcus pneumoniae YIIC (yIIC), YIID (yIID), penicillin-binding protein 2x (pbp2x), and undecaprenyi- phosphate-UDP-MurNAc-pentapeptide phospho-MurNAc-pentapeptide transferase (mraY) genes, complete 3612 8619 13629 2. 74 4.0E-01 AF068903. 1 NT cds 3737 8741 13740 3. 44 4.0E-01 AJ277511. 1 NT Ovis aries partial JD2 gene for T cell receptor delta chain (TCRDJ2), exon 1 3737 8741 13741 3. 44 4.0E-01 AJ277511.1 NT Ovis aries partial JD2 gene for T cell receptor delta chain (TCRDJ2), exon 1 I 4672 9657 8. 6 4. OE-01 Q31849 SWISSPROT NADH-PLASTOQUINONE OXIDOREDUCTASE CHAIN 5, CHLOROPLAST ! 1359 6356 11407 1. 57 3.9E-01 AF206618. 1 NT Gorilla gorilla carboxyl-ester lipase (CEL) gene, complete cds 2571 7534 12651 2. 87 3. 9E-01 AB033019. 1 NT Homo sapiens mRNA for KIAA1193 protein, partial cds 2633 7593 12705 2. 98 3. 9E-01 X82032. 1 NT H. sapiens B-myb gene 2633 7593 12706 2. 98 3. 9E-01 X82032. 1 NT H. sapiens B-myb gene 3023 8040 13049 4. 17 3.9E-01 AJ225896. 1 NT Sinorhizobium meliloti egl, syrB2, cya3 genes and orf3 3964 8962 13952 1.39 3. 9E-01 BF592611. 1 EST_HUMAN 7i61d01.x1 NCl_CGAP_Brn16 Homo sapiens cDNA clone IMAGE : 3339169 3' 159 5225 27.46 3. 8E-01 7019488 NT Homo sapiens protein kinase PKNbeta (pknbeta), mRNA 502 5537 3. 87 3. SE-01 AB029291. 1 NT Mus musculus pcm-1 mRNA for pericentrioiar material-1, complete cds 2496 7464 12579 3. 42 3. 8E-01 AF214117. 1 NT Arabidopsis thaliana putative c-myb-like transcription factor (MYB3R-3) mRNA, complete cds 2560 7775 12641 3. 09 3. 8E-01 6678002 NT Mus musculus solute carrier family 1, member 6 (Slc1a6), mRNA 2933 7952 0. 823. 8E-01 AJ251057. 1 NT Human immunodeficiency virus type 1 complete genome (isolate 98SE-MP1213) 2974 7992 13007 2. 37 3. 8E-01 AF043383. 1 NT Pieuronectes americanus aminopeptidase N @ (ampN) gene, partial cds 3403 8412 13438 8.3 3.8E-01 AL161518.2 NT Arabidopsis thaliana DNA chromosome 4, ontig fragment No. 30 Paga 16 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar SEQ ORF SEQ Expression (Top) Hit Top Hit Acession ID NO: Signal BLAST E No. Database Top Hit Descriptor Value 3461 8469 0.77 3.8E-01 AI807219.1 EST_HUMAN wf38b12.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA done) IMAGE : 2357855 3' 3671 8676 13679 0. 93 3. 8E-01 BE154080. 1 EST_HUMAN PM0-HT0339-200400-010-G01 HT0339 Homo sapiens cDNA 2410 7381 12499 5. 9 3. 7E-01 AB037831. 1 NT Homo sapiens mRNA for KIAA1410 protein, partial cds 3378 8386 13407 10. 03 3. 7E-01 AF056336. 1 NT Danio rerio bone morphogenetic protein 4 precursor (BMP4) gene, complete cds 3779 8782 13786 1. 12 3. 7E-01 AA319482. 1 EST HUMAN EST21715 Adrenal gland tumor Homo sapiens cDNA 5'end 4108 9102 14089 7. 57 3. 7E-01 AI218707. 1 EST_HUMAN ok39c07.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone IMAGE : 1510188 3' 4193 9186 14167 1. 55 3. 7E-01 AW878037. 1 EST_HUMAN MR3-OT0007-080300-104-b02 OT0007 Homo sapiens cDNA 4265 9258 14248 3. 38 3. 7E-01 AE002408. 1 NT Neisseria meningitidis serogroup B strain MC58 section 50 of 206 of the complete genome 980 5995 8. 26 3. 6E-01 U89241. 1 NT Human mibp gene, partial cds 1295 6293 11339 2. 38 3. 6E-01 T80255. 1 EST HUMAN yd03e05. r1 Soares infant brain 1 NIB Homo sapiens cDNA clone IMAGE : 24443 5' 1295 6293 11340 2.38 3. 6E-01 T80255. 3. EST_HUMAN yd03e05.r1 Soares infant T80255. 1NIB Homo sapiens cDNA clone IMAGE sapiens 24443 5' 1872 6861 11949 3. 81 3. 6E-01 AW590184. 1 EST_HUMAN hg33f02.x1 NCl_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2947419 3' 1872 6861 11950 3.81 3. 6E-01 AW590184. 1 EST_HUMAN hg33f02.x1 NCl_CGAP_GC6 Homo sapiens cDNA clone IMAGE:2947419 3' 1912 6898 11993 4. 6 3. 6E-01 AF216207. 1 NT Mus musculus ribosomal protein S19 (Rps19) gene, complete cds 2208 7185 0. 97 3. 6E-01 AB002321. 1 NT Human mRNA for KIAA0323 gene, partial cds 2326 7300 2. 62 3. 6E-01 X76725. 1 NT P. irregular (P3804) gene for actin 2417 7388 12508 0. 95 3. 6E-01 AW812033. 1 EST HUMAN RC5-ST0171-181099-011-g07 ST0171 Homo sapiens cDNA PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE (PROTEIN-BETA-ASPARTATE METHYLTRANSFERASE) (PIMT) (PROTEIN L-ISOASPARTYL METHYLTRANSFERASE) (L- 2558 7523 12639 1.1 3.6E-01 P24206 SW ISSPROT ISOASPARTYL PROTEIN CARBOXYL METHYLTRANSFERASE) 2828 10055 7. 88 3. 6E-01 AF199485. 1 NT Drosophila melanogaster 3388 8396 13420 1.83 3.6E-01 X76758. X76758. 1 NT H. sapiens serotonin transporter gene, exons 9 and 10 3388 8396 13421 1. 83 3. 6E-01 X76758. 1 NT H. sapiens serotonin transporter gene, exons 9 and 10 4284 9276 14264 0. 98 3. 6E-01 BE707883. 1 EST_HUMAN RC1-HT0545-150600-014-b12 HT0545 Homo sapiens cDNA 4610 9596 14582 1. 49 3. 6E-01 Y11526. 1 NT Z. mays mRNA for casein kinase alpha subunit 4849 9831 14806 2.79 3.6E-01 AW339393. 1 EST_HUMAN ha02g04.x1 NCl_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 2872566 3' 5037 10008 14978 1. 25 3. 6E-01 AE001187.1 NT Treponema palidum section 3 of 87 of the complete genome 115 5187 10196 0. 85 3. 5E-01 AL161536. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 36 209 5273 10285 2.22 3.5E-01 6678933 NT Mus musculus mannose receptor, C type 2 (Mrc2), mRNA 715 5738 10755 1. 11 3. 5E-01 7706136 NT Homo sapiens GAP-like protein (LOC51306), mRNA 715 5738 10756 1.11 3.5E-01 7706136 NT Homo sapiens GAP-like protein (LOC51306), mRNA 771 5793 10821 2. 75 3. 5E-01 BF129796. 1 EST HUMAN 601811060R1 NIH MGC 48 Homo sapiens cDNA clone IMAGE : 4053951 3' 1580 6577 11641 0. 92 3.5E-01 BF310688.1 EST_HUMAN 601894653F NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 4124244 5' Page 17 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database ID NO: Signal BLAST E No Top Hit Descriptor NO: NO: Source Value 1600 6596 11557 2 3.5E-01 U35776.1 NT Rattus norveglcus ADP-rlbosylation factor-directed GTPase activating protein mRNA, complete cds 2219 7196 12318 1. 43 3. 5E-01 P06798 SWISSPROT HOMEOBOX PROTEIN HOX-A4 (HOX-1. 4) (MH-3) 2531 7774 12616 1. 81 3. 5E-01 AA223252. 1 ESTHUMAN zr08a09. s1 Stratagene NT2 neuronal precursor 937230 Homo sapiens cDNA clone IMAGE : 650872 3' 3717 8721 1. 97 3. 5E-01 AA642138. 1 EST HUMAN nr60d03. s1 NCI_CGAP_Lym3 Homo sapiens cDNA clone IMAGE : 1172357 3' 4140 9135 14118 2. 06 3. 5E-01 AF071253. 1 NT Danio rerio homeobox protein (hoxb5b) gene, complete cds 4777 9761 14748 5. 2 3.5E-01 M18349.1 NT Rat leukocyte common antigen (L-CA) gene, exons 1 through 5 Homo sapiens partial N-myc (exon 3), HPV45 L2, HPV45 L1, HPV45 E6, HPV45 E7 and HPV45 E1 genes 697 5721 1. 44 3. 4E-01 AJ242956. 1 NT isolated from IC4 cervical carcinoma cell line 961 5976 11010 5. 81 3. 4E-01 Y09798. 2 NT Pseudomonas fluorescens colR, cols genes, orf222 and partial inaA gene 1306 6304 11351 1. 76 3. 4E-01 Y00554. 1 NT Azotobacter vinelandii nifA gene for NifA protein (positive regulatory element) 2338 7312 12433 1. 56 3. 4E-01 D90909. 1 NT Synechocystis sp. PCC6803 complete genome, 11/27, 1311235-1430418 2932 7951 12968 0. 8 3. 4E-01 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 2932 7951 12969 0. 8 3. 4E-01 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 3076 8092 13106 1. 04 3. 4E-01 D90909. 1 NT Synechocysts sp. PCC6803 complete genome, 11/27, 1311235-1430418 3088 8104 13119 7. 39 3. 4E-01 U83905. 1 NT Canis familiars rod photoreceptor cGMP-gated channel alpha-subunit (CNGC1) mRNA, complete cds 3268 8281 13303 1. 07 3. 4E-01 AF034862. 1 NT Homo sapiens pulmonary surfactant protein D, promoter region and exon 1 Methyiovorus sp. strain SS1 putative GrpE (grpE), DnaK (dnaK), and putative DnaJ (dnaJ) genes, complete 3453 8461 13488 4. 36 3. 4E-01 AF106835. 1 NT cds 7n94a01. x1 NCl_CGAP_Ov18 Homo sapiens cDNA clone IMAGE : 3572232 3'similar to TR : Q9UJ15 3701 8705 2. 49 3. 4E-01 BF449010. 1 EST HUMAN Q9UJ15 DJ18C9. 1 3939 8937 1. 48 3. 4E-01 AA584196. 1 EST_HUMAN no11b10.s1 NCl_CGAP_Phe1 Homo sapiens cDNA clone IMAGE : 1100347 3' 4509 9499 14478 1. 77 3. 4E-01 BE069912. 1 EST HUMAN MR4-BT0403-230200-202-c01 BT0403 Homo sapiens cDNA qj95c05. x1 NCiCGAPKid3 Homo sapiens cDNA clone IMAGE : 1867208 3'similar to contains Alu repetitive 4794 9778 4. 55 3. 4E-01 AI240973. 1 EST HUMAN element 14 5094 10078 16. 57 3. 3E-01 X07990. 1 NT Rhizobium leguminosarum sym plasmid pRL5JI nodX gene 106 5094 10078 4. 08 3. 3E-01 X07990. 1 NT Rhizobium leguminosarum sym piasmid pRL5Jl nodX gene 445 5482 10500 0. 92 3. 3E-01 AL161545. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 45 627 5655 10660 1. 67 3. 3E-01 7662485 NT Homo sapiens Kit1100 protein (KIAA1100), mRNA 1181 6183 11221 4.2 3.3E-01 Q12446 SWISSPROT PROLINE-RICH PROTEIN LAS17 1288 6287 11331 3. 14 3. 3E-01 BF568880. 1 EST HUMAN 602184016T1 NIH_MGC 42 Homo sapiens cDNA clone IMAGE : 4300251 3' 1569 6566 11628 1.18 3.3E-01 6753685 NT Mus musculus disintegrin 5 (Dtgn5), mRNA Page 18 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Database NO : NO : Value Source. Vafue 1700 6695 2. 03 3. 3E-01 AA332734. 1 EST HUMAN EST36722 Embryo, 8 week I Homo sapiens cDNA 5'end 1978 6963 1. 4 3. 3E-01 AF031148. 1 NT Methylococcus capsulatus strain Bath outer membrane protein MopB (mopB) gene complete cds Homo sapiens uridine monophosphate synthetase (orotate phosphoribosyl transferase and aotidine-5'- 2340 7314 2. 72 3. 3E-01 4507834 NT decarboxylase) (UMPS) mRNA 2g7g 7898 12921 1. 79 3. 3E-01 AJ251805. 1 NT Bacteriophage phi-Ye03-12 complete genome INTERLEUKIN-12 ALPHA CHAIN PRECURSOR (IL-12A) (CYTOTOXIC LYMPHOCYTE MATURATION 2947 7966 0. 67 3. 3E-01 002743 SW ISSPROT FACTOR 35 KD SUBUNIT) (CLMF P35) 2gg1 7999 13012 1. 05 3. 3E-01 AJ007932. 2 NT Streptomyces argillaceus mithramycin biosynthetic genes 3415 8424 13452 1. 05 3. 3E-01 AB012922. 1 NT Homo sapiens MTA1-L1 gene, complete cds 3720 8724 13724 1. 91 3. 3E-01 084645 SW ISSPROT EXODEOXYRIBONUCLEASE V BETA CHAIN GENOME POLYPROTEIN [CONTAINS : N-TERMINAL PROTEIN (P1) ; HELPER COMPONENT 3727 8731 13729 1. 01 3. 3E-01 P22602 SW ISSPROT PROTEINASE (HC-PRO) ; PROTEIN P3] 3858 8860 13866 1. 38 3. 3E-01 AL161498. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 10 3895 8895 13893 1. 71 3. 3E-01 AF200446. 1 NT Hypoxylon fragifame chifin synthasa gene, partial cds 4166 9161 2. 36 3. 3E-01 4759025 NT Homo sapiens RAS protein ac6vator like 1 (GAP1 like) (RASAL1) mRNA 4242 9236 1. 68 3. 3E-01 D31662. 1 NT Rattus norvegicus DNA fa regucalcin, partial cds tp78b12. x1 NCI_CGAP Ut3 Homo sapiens cDNA clone IMAGE : 2205407 3'similar to gb : X57522 ANTIGEN 4551 9539 1. 7 3. 3E-01 AI539114. 1 EST HUMAN PEPTIDE TRANSPORTER 1 (HUMAN) ; 4690 9675 14658 1. 36 3. 3E-01 D64003. 1 NT Synechocystis sp. PCC6803 complete genome, 22/27, 2755703-2868766 ox04g09. x1 Soares fetal liver spleen 1NFLS S1 Homo sapiens cDNA clone IMAGE : 1655392 3'similar to 5071 10040 15007 0. 98 3. 3E-01 A1021992. 1 EST HUMAN contains element MER4 repe66ve element ; 454 5491 1. 61 3. 2E-01 AF018261. 1 NT Rattus norvegicus EH domain binding protein Epsin mRNA, complete cds 708 5732 0. 78 3. 2E-01 AL161561. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 61 1144 6148 11180 10. 13 3. 2E-01 AF047013. AF047013. NT Fusarium poae virus 1 RNA2 putative RNA dependent RNA polymerase gene, compiete cds 1263 6261 11304 2. 24 3. 2E-01 Z50202. 1 NT P. wlgaris arc5-1 gene 1367 6364 11413 5. 08 3. 2E-01 Q48624 SW ISSPROT LACTOSE PERMEASE (LACTOSE-PROTON SYMPORT) (LACTOSE TRANSPORT PROTEIN) 1737 6732 11809 1. 1 3. 2E-01 Z36041. 1 NT S. cerevisiae chromosome II eading frame ORF YBR172c 1746 6741 11820 4. 36 3. 2E-01 AW957194. 1 EST HUMAN EST369264 MAGE resequences, MAGD Homo sapiens cDNA 1746 6741 11821 4. 36 3. 2E-01 AW 957194. 1 EST_HUMAN EST369264 MAGE resequences, MAGD Homo sapiens cDNA 2096 7077 12191 1. 78 3. 2E-01 BF203817. 1 EST HUMAN 601868804F1 NIH MGC_17 Homo sapiens cDNA clone IMAGE : 4111512 5' 2470 7438 2. 59 3. 2E-01 7710079 NT Mus musculus Pbxlknotted 1 homeobox (Pknox1), mRNA 2637 7597 12711 1. 11 3. 2E-01 AF060568. 1 NT Homo sapiens promyelocytic leukemia zinc finger protein (PLZF) gene, complete cds 3045 8062 13070 1. 07 3. 2E-01 BF380745. 1 EST HUMAN IL2-UT0073-180900-161-H11 UT0073 Hano sapiens cDNA Page 19 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Simiar Top Hit SEQ ID SEQ Ip RF SEQ Expression (Top) Hit Top Hit Acession pabase Top Hit Descriptor NO : NO : ID N0 : Signal BLAST E No. Source, Value Rabbit beta-like globin gene cluster encoding the epsion, gamma, delta (pseudogene) and beta globin 4270 9263 14253 1. 73 3. 2E-01 M18818. 1 NT poypeptides, complete cds 4369 9362 14342 1. 43 3. 2E-01 Q10268 SW ISSPROT HYPOTHETICAL 81. 7 KD PROTEIN C13G7. 04C IN CHROMOSOME I PRECURSOR 4597 9585 7. 8 3. 2E-01 BF693617. 1 EST HUMAN 602081972F1 N ! H MGC 81 Homo sapiens cDNA clone IMAGE : 4246505 5' 5054 10025 14994 1. 02 3. 2E-01 AL161514. 2 NT Arabidopsis thaliana DNA chromosome 4, config fragment No. 26 ye90h06. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 125051 5'similar to 2600 7562 12680 2. 32 3. 1E-01 R18051. 1 EST HUMAN gb : M64241 QM PROTEIN (HUMAN) ; 2625 7709 12696 2. 72 3. 1E-01 7661971 NT Homo sapiens KIAA0174 gene product (KIAA0174), mRNA 2625 7709 12697 2. 72 3. 1E-01 7661971 NT Homo sapiens KIAA0174 gene product (KIAA0174), mRNA 2785 7806 1. 27 3. 1E-01 AW629036. 1 EST HUMAN hi46h08. x1 Soares NFL_T_GBC S1 Homo sapiens cDNA clone IMAGE : 2975391 3' 3100 8116 3. 43 3. 1 E-01 AB029069. 1 NT Mus musculus gene for SedThr kinase KKIAMRE, exon 6 3811 8814 13820 0. 89 3. 1 E-01 AJ251586. 9 NT Daucus carota mRNA for transcription factor E2F (E2F gene) 4806 9790 14772 5. 69 3. 1 E-01 AE003984. 1 NT Xylella fastidiosa, section 130 aF 229 of the complete genome 71 7689 10158 1. 59 3. OE-01 6755083 NT Mus musculus protein kinase C, epsilon (Pkce), mRNA 252 5312 10323 9. 02 3. OE-01 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1l2 1204 6205 11242 1. 55 3. OE-01 AW300400. 1 EST NUMAN xs63f08. c1 NCI_CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2774343 3' 1478 6475 11532 3. 47 3. OE-01 AJ006755. 1 NT Balaenoptera physalus gene encoding atrial natriuretic peptide 1760 6752 11837 1. 03 3. OE-01 X99082. 1 NT A. immersus putative gene encoding integrase, Mars2 (RP) 3139 8155 4. 06 3. OE-01 AB030481. 1 NT Corynebacterium sp. ALY-1 alyPG gene for polyguluronate lyase, complete cds 3774 8777 13781 1. 61 3. OE-01 AW817785. 9 EST HUMAN PM9-ST0262-261199-001-g01 ST0262 Homo sapiens cDNA _ 3867 8868 13871 0. 98 3. OE-01 AJ271736. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 2/2 ; 4382 9373 14352 2. 26 3. OE-01 AJ006755. 1 NT Balaenoptera physalus gene encoding atrial natriuretic peptide. 4589 9577 0. 99 3. OE-01 AF157835. 1 NT Bacteriophage APSE-1, complete genome 1973 6958 12063 1. 16 2. 9E-01 AE000736. 1 NT Aquffex aeolicus section 68 of 109 of the complete genome 3110 8126 13146 1. 04 2. 9E-01 AF078111. 1 NT Xenopus laevis transcription factor E2F mRNA, complete cds 3178 8194 13216 2. 82 2. 9E-01 AW754239. 1 EST HUMAN PM1-CT0326-171299-001-f12 CT0326 Homo sapiens cDNA 3178 8194 13217 2. 82 2. 9E-01 AW754239. 1 EST HUMAN PM1-CT0326-171299-001-f12 CT0326 Homo sapiens cDNA tp21a11. x1 NCI CGAP Gas4 Homo sapiens cDNA clone IMAGE : 2188412 3'similar to gb : D15050 NIL-2-A 3801 8804 13809 0. 7 2. 9E-01 AI610836. 1 EST HUMAN ZINC FINGER PROTEIN (HUMAN) ; contains element L1 repetitive elemenf ; 3959 8957 13946 12. 27 2. 9E-01 AB016426. 1 NT Cavia porcellus mRNA for glutathione s-transferase, complete cds zs57d12. r1 NCI_CGAP GCB1 Homo sapiens cDNA clone IMAGE : 701591 5'similar to contains Alu 4359 9350 14329 1. 05 2. 9E-01, 4A284468. 1 EST HUMAN repe6tive element ; 4545 9534 0. 85 2. 9E-01 AL163207. 2 NT Homo sapiens chromosome 21 segment HS21 C007 : z a. Page 20 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Value wa06f03. x1 NCl_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2297309 3'similar to contains L1. t2 L1 4946 9923 1. 33 2. 9E-01 AI670899. 1 EST HUMAN repetitive element ; 5020 9991 1. 01 2. 9E-01 AL161585. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 81 563 5597 2. 11 2. 8E-01 U67136. 1 NT Rattus norvegicus A-kinase anchoring protein AKAP150 mRNA, complete cds 568 5601 0. 7 2. 8E-01 L28145. 1 NT Prune dwarf virus movement protein, complete cds ; coat protein, complete cds 1067 6075 11108 2. 54 2. 8E-01 AF168050. 1 NT Guira guia oocyte maturation factor Mos (c-mos) gene, partial cds 1258 6256 11298 0. 98 2. 8E-01 BE313442. 1 EST_HUMAN 601148733F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 3163688 5' 1258 6256 11299 0. 98 2. 8E-01 BE313442. 1 EST_HUMAN 601148733F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 3163688 5' 1272 6270 11310 0. 92 2. 8E-01 D86550. 1 NT Human mRNA for serinelthreonine protein kinase, complete cds 1689 6685 11761 2. 03 2. 8E-01 AW860020. 1 ESTHUMAN QV1-CT0364-120200-065-b05CT0364 Homo sapiens cDNA 1962 6948 12050 1. 44 2. 8E-01 AL047620. 1 EST HUMAN DKFZp58612321 r1 586 (synonym : hutel) Homo sapiens cDNA clone DKFZp58612321 2073 7055 12165 1 2. 8E-01 AW511195. 1 ESTHUMAN hd44b03. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2912333 3' 2401 7372 12493 2. 06 2. 8E-01 AE000494. 1 NT Escherichia coli K-12 MG1655 section 384 of 400 of the complete genome 2401 7372 12494 2. 06 2. 8E-01 AE000494. 1 NT Escherichia coli K-12 MG1655 section 384 of 400 of the complete genome 2476 7445 1. 49 2. 8E-01 AL161565. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 65 2594 7557 12671 1.4 2.8E-01 AB020975. 1 NT Arabidopsis thaliana mRNA for lipoyltransferase, complete cds 2901 7920 1. 35 2. 8E-01 AF179480. 1 NT Toxoplasma gondii 90kDa heat-shock protein (HSP90) mRNA, partial cds 2902 7921 12941 2. 3 2. 8E-01 Z14037. 1 NT B. taurus microsatellite (ETH121) 2902 7921 12942 2. 3 2. 8E-01 Z14037. 1 NT B. taurus microsatellite (ETH121) 32948305 13330 0. 85 2. 8E-01 AP000004. 1 NT Pyrococcus horikoshii OT3 genomic DNA, 777001-994000 nt. position (4/7) 3890 8890 13888 1. 44 2. 8E-01 AE001180. 1 NT Borrelia burgdorferi (section 66 of 70) of the complete genome 4005 9001 0. 91 2. 8E-01 AE004450. 1 NT Pseudomonas aeruginosa PA01, section 11 of 529 of the complete genome ov44g10. x1 SoarestestisNHT Homo sapiens cDNA clone IMAGE : 1640226 3'similar to contains Alu 4075 9069 2. 36 2. 8E-01 A1090868. 1 EST_HUMAN repetitive element ; contains element MER22 repetitive element ; 4337 9328 14313 2. 15 2. 8E-01 P13615 SWISSPROT RNA POLYMERASE BETA SUBUNIT (LARGE STRUCTURAL PROTEIN) (L PROTEIN) 4682 9667 14649 2. 85 2. 8E-01 AF030154. 1 NT Bovine adenovirus 3 complete genome 4711 9696 14680 1. 28 2. 8E-01 BF528188. 1 EST HUMAN 602042601 F1 NCl_CGAP_Brn67 Homo sapiens cDNA clone IMAGE : 4180129 5' ql59c11.x1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE : 1876628 3'similar to contains Alu 4731 9716 14701 2. 3 2. 8E-01 AI272669. 1 EST HUMAN repetitive element ; contains element LTR5 repetitive element ; 5004 9975 14950 1. 68 2. 8E-01 AA767084. 1 EST_HUMAN oa41hO1. s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE : 1307569 3' 473 5509 10522 2. 82 2. 7E-01 Y17324. 1 NT Rattus norvegicus CDK104 mRNA zx39b10. s1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE : 788827 3'similar to 609 5636 10637 2. 19 2. 7E-01 AA450061. 1 EST HUMAN contains Alu repetitive element ; 1242 6240 11281 1. 57 2. 7E-01 AB004906. 1 NT Ipomoea purpurea transposable element Tip100 gene for transposase, complete cds Page 21 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Source NO: NO: Source Value 1582 6579 1.3 2.7E-01 X79815.1 NT G.lamblia SR2 gene 1691 6687 11762 2. 21 2. 7E-01 W58067.1 EST HUMAN zd22h10.r1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone MAGE : 341443 5' GAG POLYPROTEIN [CONTAINS : INNER COAT PROTEIN P12 ; CORE PROTEIN P15 ; CORE SHELL 1739 6734 11811 1. 16 2. 7E-01 P03341 SWISSPROT PROTEIN P30 ; NUCLEOPROTEIN P10] 2077 7762 1. 42 2. 7E-01 AF047575. 1 NT Rattus norvegicus vesicular monoamine transporter type 2, promoter region and exon 1 2306 7281 12399 6. 34 2. 7E-01 Y13868. 1 NT Feline immunodeficiency virus env gene, isolate ITT0088PIU (M88), partial ta43c11.x2 NIH_MGC_Lu25 Homo sapiens cDNA clone IMAGE : 2046836 3'similar to contains element L1 2386 7357 12479 2.78 AI928015. 2. 7E-01 AI310858. 1 EST HUMAN repetitive element ; 2915 7934 0. 72 2. 7E-01 BF088284. 1 ESTHUMAN CM1-HT0875-060900-385-e05HT0875 Homo sapiens cDNA 3904 8904 13902 1. 98 2. 7E-01 1 1 EST HUMAN wo92e11. x1 NCI CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2462828 3' 3918 8918 13911 2. 26 2. 7E-01 L77569. 1 NT Homo sapiens DiGeorge syndrome critical region, telomeric end 4739 9724 14709 1. 14 2. 7E-01 L27516. NT TYriticum aestivum (Wcs66) gene, complete cds 4875 9854 4. 25 2. 7E-01 AW856131. 1 EST HUMAN RC1-CT0286-230200-01&e03 CT0286 Homo sapiens cDNA 467 7721 10516 1. 71 2. 6E-01 P78411 SWISSPROT IROQUOIS-CLASS HOMEODOMAIN PROTEIN IRX-2 477 5514 1. 09 2. 6E-01 D16459. 1 NT Bos taurus mRNA for mb-1, complete cds 1370 6367 11416 1. 36 2. 6E-01 BE885087. 1 EST HUMAN 601510838F1 NIH MGC 71 Homo sapiens cDNA clone IMAGE : 3912345 5' 1410 6408 11466 0. 92 2. 6E-01 AB013290. 1 NT Glycine max pseudogene for Bd 30K 1856 6845 11932 4. 92 2. 6E-01 AL161472. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 2 1856 6845 11933 4. 92 2. 6E-01 AL161472. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 2 bb04d10. x1 NIH_MGC_14 Homo sapiens cDNA clone IMAGE : 2958451 3'similar to gb : M36072 60S RIBOSOMAL PROTEIN L7A (HUMAN) ; gb : M14689_cds1 Mouse surfeit locus surfeit 3 protein gene 2037 70207. 2. 6E-01 AW733152. 1 EST HUMAN (MOUSE) ; 2092 7073 12187 1. 04 2. 6E-01 M11844. 1 NT Human prealbumin gene, complete cds 2402 7373 3. 5 2. 6E-01 Y12996. 1 NT B.marltimus rbcL gene 2475 7444 5. 05 2. 6E-01 BE272440. 1 EST HUMAN 601126016F1 NIH_MGC_9 Homo sapiens cDNA clone IMAGE : 2990043 5' 3018 8035 1. 09 2. 6E-01 AW974531. 1 EST HUMAN EST386635 MAGE resequences, MAGM Homo sapiens cDNA 3558 8565 13572 2. 15 2. 6E-01 AF229118. 1 NT Homosapiens acetyicholinesterasecdlagen-like tail subunit (COLQ) gene, exons 1A 2 3 4 and 5 3983 8981 13966 0. 94 2. 6E-01 AW959510. 1 EST-HUMAN EST371580 MAGE resequences, MAGF Homo sapiens cDNA 4031 9027 14016 17. 72 2. 6E-01 BE080598. 1 EST HUMAN QV1-BT0630-040400-132-e03 BT0630 Homo sapiens cDNA Enterococcus faecium strain N97-330 vanD glycopepfide resistance gene cluster, complete cds ; and 4231 9225 14208 1. 2 2. 6E-01 AF175293. 1 NT unknown gene 4417 9407 14394 1. 2 2. 6E-01 AA457617. 1 EST HUMAN aa89d07. r1 Stratagene fetal retina 937202 Homo sapiens cDNA clone IMAGE : 838477 5' 4512 9502 14481 2. 55 2.6E-01 U01103.1 NT Arabidopsis thaliana PSI type III chlorophyll a/b-binding protein (Lhca3*1) mRNA, complete cds Page 22 of 209 Table 4 Single Exon Probes Expressed in HBL1Q0 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession @@@ @@ @@@ @@ @@@@@@@@ SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Value 4577 9565 14553 1. 46 2. 6E-01 AF142703. 1 NT Ophrestia radicosa maturase-like protein (matiS) gene, complete cds ; chloroplast gene for chloroplast produce 4812 9796 14779 3.7 2.6E-01 H04858.1 EST_HUMAN yj51e05.r1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 152288 5' 4877 9856 1.53 2.6E-01 AA884625. 1 ESTHUMAN am33b11. s1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 1468605 3' 5069 10038 15005 1.24 2. 6E-01 M37701. 1 NT P. chrysosporium lignin peroxidase genes, complete cds Homo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit (ATP5D), nuclear 239 5301 10311 1. 57 2.5E-01 4502296 NT gene encoding mitochondrai protein, mRNA Homo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit (ATP5D), nuclear 240 5301 10311 2. 3 2. 5E-01 4502296 NT gene encoding mitochondrial protein, mRNA 253 5313 7. 98 2. 5E-01 M26501. 1 NT Starfish (P. ochraceus) cytoplasmic actin gene, complete cds 822 5842 10878 0. 98 2. 5E-01 U09964. 1 NT Mus musculus ICR/Swiss glyceraldehyde 3-phosphate dehydrogenase (Gapd-S) gene, complete cds 1043 6053 0. 73 2. 5E-01 AE002156. 1 NT Ureaplasma urealyticum section 57 of 59 of the complete genome 1104 6111 11142 14. 95 2.5E-01 T89837. 1 EST_HUMAN ye11g07.r1 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE : 117468 5' 1690 6686 3. 61 2.5E-01 4885406 NT Homo sapiens hyperpotarization activated cyclic nucleode-gated potassium channel 4 (HCN4) mRNA 1844 7757 11922 1. 29 2. 5E-01 BE696604. 1 EST HUMAN PM4-CT0400-310700-005-d08 CT0400 Homo sapiens cDNA 1844 7757 11923 1. 29 2. 5E-01 BE696604. 1 EST_HUMAN RM4-CT0400-310700-005-d08 CT0400 Homo sapiens cDNA 2342 7316 6. 55 2. 5E-01 AE000675. 1 NT Aquifex aeolicus section 7 of 109 of the complete genome 2565 7528 12646 12. 5E-01 X95310. 1 NT B. taurus mRNA for D-aspartate oxidase 3328 8338 3. 33 2. 5E-01 AW973471. 1 EST HUMAN EST385464 MAGE resequences, MAGM Homo sapiens cDNA 3457 8465 13492 1. 12 2. 5E-01 AF233875. 1 NT Danio rerio peptide YY precursor gene, complete cds 3473 8481 13500 6.31 2. 5E-01 AL161517. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 29 3757 8760 13759 1.55 2.5E-01 AI741483. 1 EST_HUMAN wg11c07.x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone IMAGE : 2364780 3' 3757 8760 13760 1. 55 2. 5E-01 AI741483. 1 EST_HUMAN wg11c07x1 Soares_NSF_F8_9W_OT_PA_P_S1 Homo sapiens cDNA clone IMAGE:2364780 3' 3955 8953 1. 09 2. 5E-01 P32323 SWISSPROT A-AGGLUTININ ATTACHMENT SUBUNIT PRECURSOR 4191 9184 0. 91 2. 5E-01 Q03314 SWISSPROT RHIB PROTEIN 4606 9594 1. 31 2. 5E-01 Q27225 SWISSPROT MOLT-INHIBITING HORMONE PRECURSOR (MIH) 4611 9597 14583 4. 67 2. 5E-01 AF007768. 1 NT Choristoneura fumiferana diapause associated protein 2 (DAP2) mRNA, complete cds 4637 9622 14614 2. 24 2.5E-01 AE004416. 1 NT Vibrio chderae chromosome 11, section 73 of 93 of the complete chromosome Mus musculus annexin V gene, intron 4 segment containing 5'LTR and gag portion of MuERV-L (murine 4658 9643 3.61 2.5E-01 AJ230113. 1 NT endogenous retrovirus) element 4722 9707 14694 0. 8 2.5E-01 U83656. 1 NT Rattus norvegicus NF-KB gene, promotor region 549 5583 10585 1. 12 2. 4E-01 AA936316. 1 EST-HUMAN on70d04. s1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 1562023 3' Page 23 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database ID NO: Signal BLAST E No Top Hit Descriptor Va ! use 838 5857 10898 1. 73 2. 4E-01 BF576124. 1 EST HUMAN 602132442F1 NIH MGC 81 Homo sapiens cDNA clone IMAGE : 4271578 5' 1285 6284 11326 14. 3 2. 4E-01 AJ289880. 1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and LZTFL1 gene 1285 6284 11327 14. 3 2. 4E-01 AJ289880. 1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and LZTFL1 gene 1814 6804 14. 57 2.4E-01 AF267753.1 NT Mesembryanthemum crystallinum putative potassium channel protein Mkt1p mRNA, complete cds 1859 6848 11936 1. 09 2. 4E-01 AF251708. 1 NT Zaocys dhumnades fructose-1, 6-bisphosphatase mRNA, complete cds 2104 7084 1. 04 2. 4E-01 P45384 SWISSPROT IMMUNOGLOBULINA1 PROTEASEPRECURSOR (IGA1 PROTEASE) 2200 7178 12301 1. 86 2. 4E-01 AE000680. 1 NT Aquifex aeoXicus section 12 of 109 of the complete genome _ 7h23dO4. x1 NCl_CGAP_Co16 Homo sapiens cDNA clone IMAGE : 3316807 3'similar to SW : PRSB XENLA 2320 7295 12415 131. 89 2. 4E-01 BF002171. 1 EST HUMAN 042586 26S PROTEASE REGULATORY SUBUNIT 6A ; 2467 7436 12553 2. 1 2. 4E-01 Z36534. 1 NT D. discoideum (Ax3-K) ponA gene 2688 7646 12761 1.53 2.4E-01 X71783.1 NT S.pombe swi6 gene 2713 7670 12783 3. 6 2. 4E-01 AF030154. 1 NT Bovine adenovirus 3 complete genome 3059 8076 3. 61 2. 4E-01 U72726. 1 NT Oryza longistaminata receptor kinase-like protein, family member D, and retrofit (gag/pol) genes, complete cds 3075 8091 13105 1. 58 2. 4E-01 X74209. 1 NT H. sapiens AGT gene, Pstl fragment of intron 4 3675 8680 13683 0. 71 2. 4E-01 AE000312. 1 NT Escherichia coli K-12 MG1655 section 202 of 400 of the complete genome 3920 8920 0. 95 2. 4E-01 D29960. 1 NT Rattus norvegicus mRNA for alphaB crystallin-related protein, complete cds 4986 9960 14937 1. 38 2. 4E-01 AF252302. 1 NT Oncorhynchus mykiss shaker-related potassium channel Tsha2 gene, compjete cds 388 5426 10441 0. 83 2. 3E-01 S75898. 1 NT aromatase [Poephila guttata=zebra finches, ovary, mRNA, 3188 nt] 631 5659 4.7 2.3E-01 U39713.1 NT Mycoplasma genitalium section 35 of 51 of the complete genome 659 5686 10693 27. 33 2. 3E-01 U67596. 1 NT Methanococcus jannaschii section 138 of 150 of the complete genome 921 5937 10968 3. 41 2. 3E-01 BE311893. 1 EST_HUMAN 601142073F1 NIH_MGC_14 Homo sapiens cDNA clone IMAGE : 3505818 5' 1592 6588 11650 2. 09 2. 3E-01 Y10887. 2 NT Mus musculus cdh5 gene, exon 1, partial 1994 6978 1. 08 2. 3E-01 AJ235353. 1 NT Homo sapiens partial intron 3 of the wild type AF-4/FEL gene 2378 7350 12470 1. 58 2. 3E-01 BE297718. 1 EST_HUMAN 601175562F1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE : 3531015 5' 2579 7542 12656 0.92 2.3E-01 M1319. 3E-01 M11319. gene, complete Human 2751 6362 11411 1. 97 2. 3E-01 AB015033. 1 NT Marinilabilia agarovorans gyrB gene for DNA gyrase subunit B, partial cds, strain : lFO 14957 no16d06.s1 NCl_CGAP_Phe1 Homo sapiens cDNA clone IMAGE : 1100843 3'similar to contains Alu 2893 7912 12933 0. 83 2. 3E-01 AA601379. 1 EST HUMAN repetitive element ; contains element THR repetitive element ; 3010 8027 6. 01 2. 3E-01 R21732. 1 EST_HUMAN yh21b07.s1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 130357 3' 3216 8231 13252 0. 91 2. 3E-01 U65391. 1 NT Lycopersicon esculentum PRF (Prf) gene, complete cds 3288 8299 13326 1. 23 2.3E-01 H69836. 1 EST HUMAN yr97h10. r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 213283 5' 3832 8834 5. 25 2. 3E-01 7662133 NT Homo sapiens KIAA0450 gene product (KIAA0450), mRNA 4224 9218 14197 6.38 2.3E-01 R82252. 1 EST_HUMAN yj17f01.r1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE:149017 5' Page 24 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database ID NO: Signal BLAST E No Top Hit Descriptor @@@ @@@ @@@@@@ NO : NO : Value Source 4277 9270 1. 85 2. 3E-01 L78789. 1 NT Mus musculus renin (Ren-1c) gene, promoter region 4329 9320 14305 0.87 2.3E-01 D90899. 1 NT Synechocystis sp. PCC6803 complete genome, 1/27, 1-133859 4360 9351 14330 2.08 2. 3E-01 AF092535. 1 NT Homo sapiens mitogen-activated protein kinase p38delta (PRKM13) mRNA, complete cds 4422 9412 14400 5.79 2.3E-01 5031984 NT Homo sapiens nuclear transport factor 2 (placental protein 15) (PP15) mRNA 4882 9861 14832 0. 69 2. 3E-01 AB032400. 1 NT Mus musculus tulip 1 mRNA, complete cds oz14a10.x1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 1675290 3'similar to 88 5165 10176 0. 8 2. 2E-01 AlO52190. 1 EST HUMAN TR : Q13040 Q13040 ATP-BINDING CASSETTE PROTEIN ; 1532 6530 11590 2. 42 2. 2E-01 AF187850. 1 NT Homo sapiens PPAR delta gene, promoter region 1969 6954 1. 16 2. 2E-01 AF171901. 1 NT Trimeresurus malabaricus cytb gene, partial cds ; mitochondrial gene for mitochondrial product 2035 7018 12128 1. 86 2. 2E-01 M34640. 1 NT Fresh-water sponge Emf1 alpha collagen (COLF1) gene 2339 7313 12434 4. 2 2. 2E-01 BF677538. 1 EST_HUMAN 602085608F1 NIH_MGC_83 Homo sapiens cDNA clone IMAGE : 4249969 5' 2512 7480 12595 1.38 2. 2E-01 BE618258.1 EST_HUMAN 601462629F1 NIH_MGC_67 Homo sapiens cDNA clone IMAGE : 3866190 5' 2512 7480 12596 1. 38 2. 2E-01 BE618258. 1 EST_HUMAN 601462629F1 NIH_MGC_67 Homo sapiens cDNA clone IMAGE : 3866190 5' 2812 7832 12848 5.48 2. 2E-01 BE155625. 1 ESTHUMAN PM2-HT0353-281299-003-a12 HT0353 Homo sapiens cDNA 2812 7832 12849 5.48 2. 2E-01 BE155625. 1 EST HUMAN PM2-HT0353-281299-003-a12 HT0353 Homo sapiens cDNA 2850 7870 1. 44 2. 2E-01 AF020503. 1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydroiase (FHlT) gene, exon 5 3308 8319 2.3 2.2E-01 AL161562.2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 62 3667 8672 13676 1. 51 2. 2E-01 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 3724 8728 1. 61 2. 2E-01 AF155728. 1 NT Xiphophorus maculatus truncated Rex1 retrotransposon reverse transcriptase (RT) pseudogene 4091 9085 1. 09 2. 2E-01 AF119102. 1 NT Drosophila melanogaster UNC-119 (unc-119) gene, complete cds Mus musculus mixed lineage kinase 3 (Mlk3) and two pore domain K+ channel subunit (Kcnk6) genes, 4098 9092 14078 6. 31 2. 2E-01 AF155142. 1 NT complete cds 4141 9136 14119 2. 24 2. 2E-01 AF117340. 1 NT Mus musculus MAP kinase kinase kinase 1 (Mekk1) mRNA, complete cds 4141 9136 14120 2.24 2.2E-01 AF117340.1 NT Mus musculus MAP kinase kinase kinase 1 (Mekk1) mRNA, complete cds 4230 9224 14206 1. 24 2. 2E-01 U01307. 1 NT Human scRNA (BC200 beta) pseudogene ** 4230 9224 14207 1. 24 2. 2E-01 U01307. 1 NT Human scRNA (BC200 beta) pseudogene 4679 9664 1. 4 2. 2E-01 D50604. 1 NT Human beta-cytoplasmic actin (ACTBP9) pseudogene 4684 9669 14651 2. 15 2. 2E-01 AA211216. 1 EST HUMAN zq87c05. r1 Stratagene hNT neuron (#937233) Homo sapiens cDNA clone IMAGE : 648968 5' 4878 9857 1.24 2. 2E-01 L13299. 1 NT Mus musculus vinculin gene, exon 3 yr42h09. r1 Scares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 208001 5'similar to 4961 9938 14915 0.91 2.2E-01 H60548. 1 EST HUMAN gb : Z14116_ma1 CD59 GLYCOPROTEIN PRECURSOR (HUMAN) ; 956 5972 11006 1.5 2.1E-01 AA569289. 1 EST HUMAN nm31e11. s1 NCI CGAP Lip2 Homo sapiens cDNA clone IMAGE : 1061804 959 5974 11008 1.79 2.1E-01 AL161504. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 16 Page 25 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database @@ @@@ @@@@@@ @@@@@ @ @@ Top Hit Descriptor NO : NO : Sourie Valut 1107 6113 2.15 2.1E-01 AE002314. 2 NT Chlamydia muridarum, section 45 of 85 of the complete genome 1179 6181 11217 1. 22 2. 1E-01 6754299 NT Mus musculus interferon (alpha and beta) receptor 2 (Ifnar2), mRNA 1179 6181 11218 1. 22 2.1E-01 6754299 NT Mus musculus interferon (alpha and beta) receptor 2 (Ifnar2), mRNA ok73e02. s1 NCl CGAP GC4 Homo sapiens cDNA clone IMAGE : 1519610 3' similar to gb : K02765 1869 6858 11946 1.52 2.1E-01 AA906824. 1 EST HUMAN COMPLEMENT C3 PRECURSOR (HUMAN) ; 2094 7075 12189 2.42 2.1E-01 BF695073. 1 EST_HUMAN 602083129F1 NIH_MGC_81 Homo sapiens cDNA clone IMAGE : 4247503 5' 2854 7874 12889 2. 25 2. 1E-01 6912M5 NT Homo sapiens potassium voltage-gated channel, subfamily H (eag-related), member 4 (KCNH4), mRNA 3718 8722 6. 22 2.1E-01 9838361 NT Beta vulgaris mitochondrion, complete genome 3940 8938 13930 1. 28 2.1E-01 P11675 SWISSPROT IMMEDIATE-EARLY PROTEIN IE180 3940 8938 13931 1. 28 2. 1 E-01 P11675 SW ISSPROT IMMEDIATE-EARLY PROTEIN IE180 4128 9123 0.9 2.1E-01 AF124526. 1 NT Orchestia cavimana calcium-binding protein BP23 precursor (BP23) gene, complete cds 4251 9245 1. 2E 2. 1E-01 AB033041. 1 NT Homo sapiens mRNA for KIAA1215 protein, partial cds 4443 9433 14416 1.66 2.1E-01 AB010273. 1 NT Homo sapiens pshsp47 gene, complete cds 4719 9704 1469042. 1E-01 AJ009794. 1 NT Homo sapiens hox11 proto-oncogene, exons 1 to 3 and hug-1 gene GENOME POLYPROTEIN [CONTAINS : CAPSID PROTEIN C (CORE PROTEIN) (P22) ; ENVELOPE GLYCOPROTEIN E1 (GP32) (GP35) ; ENVELOPE GLYCOPROTEIN E2 (GP68) (GP70) (NS1) ; PROTEIN P7 ; NONSTRUCTURAL PROTEIN NS2 (P21) ; PROTEASE/HELICASE NS3 (P70) ; NONSTRUCTURAL 5030 10001 0. 92 2.1E-01 P26660 SWISSPROT PROTEIN 5067 10036 15003 1 2.1E-01 U02948. 1 NT Human surfactant protein-C (SP-C) gene, complete cds 200 5264 10278 2. 64 2.0E-01 AB017437. 1 NT Gallus gallus mRNA for avena, complete cds 530 5565 2. 17 2. OE-01 7705601 NT Homo sapiens CGI-18 protein (LOC51008), mRNA 690 5714 10728 1. 32 2.0E-01 M77085. 1 NT O. cunniculus germline IgH heavy chain V-H pseudogene, allotype VHa2 801 5822 10852 5. 78 2. OE-01 AF027865. 1 NT Mus musculus Major Histocompatibility Locus class 11 region 996 6008 11038 0.66 2.0E-01 D90905.1 NT Synechocystis sp. PCC6803 complete genome, 7/27, 781449-920915 1108 6114 11144 2. 91 2. OE-01 AL163213. 2 NT Homo sapiens chromosome 21 segment HS21 C013 1235 6233 11277 1.34 2.0E-01 AJ132695. 5 NT Homo sapiens rac1 gene 1289 6288 11332 1. 08 2. 0E-01 AW384937. 1 EST HUMAN PM1-HT0422-291299-002-c06 HT0422 Homo sapiens cDNA 1432 6429 1. 01 2.0E-01 AJ243957. 1 NT Plum pox virus strain M, complete genome, isolate PS 1456 6453 11512 7. 19 2.0E-01 4503508 NT Homo sapiens dystrobrevin, alpha (DTNA), mRNA 1520 6517 11574 3. 81 2. OE-01 AB007974. 1 NT Homo sapiens mRNA, chromosome 1 specific transcript KIAA0505 1526 6523 11580 1.54 2.0E-01 AF260700. 1 NT Homo sapiens sodiumliodide symporter mRNA, partial cds 1658 6654 11726 1.48 2.0E-01 U22346. 1 NT Human bradykinin B1 receptor (bradyb1) gene, complete cds 1679 6675 1.48 2.0E-01 AF111170.3 NT Homo sapiens 14q32 Jagged2 gene, complete cds ; and unknown gene Page 26 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells , Probe Exon Most Similar Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor NO Value Source Value 1720 6715 3. 96 2. OE-01 U67525. 1 NT Methanococcus jannaschii section 67 of 1 50 of the complete genome 1853 6842 11930 0. 93 2. OE-01 8922238 NT Homo sapiens hypothetical protein FLJ10120 (FLJ10120), mRNA 2286 7262 1. 21 2. OE-01 X82877. 1 NT H. sapiens Na+-D-glucose cotransport regulator gene HOMEOBOX PROTEIN GLABRA2 (HOMEOBOX-LEUCINE ZIPPER PROTEIN ATHB-10) (HD-ZIP 3407 8416 13444 0.68 2.0E-01 P46607 SWISSPROT PROTEIN ATHB-10) xp15b02.x1 NCI_CGAP_HN9 Homo sapiens cDNA clone IMAGE : 2740395 3'similar to contains element 3489 849 0. 67 2. OE-01 AW238005. 1 EST HUMAN MER21 repetitive element; 3624 8631 13637 0. 7 2. OE-01 P34641 SWISSPROT CED-11 PROTEIN 3919 8919 13912 0.77 2.0E-01 X83997. 1 NT C. parasitica eapC gene 4433 9423 9.72 2.0E-01 BE826165. 1 EST HUMAN QV4-EN0032-190500-223-e03 EN0032 Homo sapiens cDNA 4874 9853 14828 7.34 2.0E-01 8922080 NT Homo sapiens hypothetical protein ASH1 (ASH1), mRNA 4931 9908 14885 0. 93 2. 0E-01 Y19216. 1 NT Homo sapiens putative psihHbD pseudogene for hair keratin, exons 1 to 9 4979 7840 7.46 2.0E-01 AF074990. 1 NT Homo sapiens full length insert cDNA YH85A11 5045 10016 14985 1.19 2.0E-01 AF197159. 1 NT Mus musculus cubilin mRNA, partial cds 5059 10029 1. 29 2. OE-01 T47785. 1 EST_HUMAN yb17a10.r1 Stratagene fetal spleen (#937205) Homo sapiens cDNA clone IMAGE:71418 5' 110 5182 9. 3 1. 9E-01 7549743 NT Rattus norvegicus Aryl hydrocarbon receptor nuclear translocator 1 (Arnt1), mRNA 350 5402 10412 5. 34 1.9E-01 AF004353. 1 NT Mus musculus pate ear (ep) gene, wild type allele, 3'region, part'al cds 648 5676 10682 24. 68 1. 9E-01 U32581. 2 NT Homo sapiens lambda/iota protein kinase C-interacting protein mRNA, complete cds 648 5676 10683 24. 68 1. 9E-01 U32581. 2 NT Homo sapiens lambda/iota protein kinase C-interacting protein mRNA, complete cds 655 5683 10690 6. 04 1. 9E-01 BE070801. 1 EST_HUMAN RC3-BT0502-251199-011-d01 BT0502 Homo sapiens cDNA 656 5683 10690 5. 32 1. 9E-01 BE070801. 1 EST_HUMAN RC3-BT0502-251199-011-d01 BT0502 Homo sapiens cDNA 971 5986 0. 96 1. 9E-01 7305180 NT Mus musculus interleukin 2 receptor, gamma chain (II2rg), mRNA 1087 6094 11124 12. 01 1.9E-01 AA358813. 1 EST_HUMAN EST67784 Fetal lung II Homo sapiens cDNA 5' end 1353 6350 11400 3. 22 1. 9E-01 AF061282. 1 NT Sorghum bicolor 22 kDa kafirin cluster 1409 6407 2. 64 1. 9E-01 AF184623. 1 NT Plasmodium vivax reticulocyte binding protein-2 (rbp-2) gene, complete cds 2319 7294 12414 2. 22 1. 9E-01 8922533 NT Homo sapiens hypothetical protein FLJ10581 (FLJ10581), mRNA 2852 7872 12887 3. 91 1. 9E-01 U66066. 1 NT Sigmodon hispidus p53 gene, partial cds 2868 7887 5. 5 1.9E-01 J00922.1 NT Gallus gallus ovalbumin (Y) gene, complete cds 3311 8322 13345 4. 28 1. 9E-01 D13197. 1 NT Mouse gene for immunoglobulin diversity region D1 3399 8408 13434 4. 85 1.9E-01 R16467. 1 EST HUMAN yf42f10. r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 129547 5' 3716 8720 13721 0. 77 1. 9E-01 AF264017. 1 NT Rattus norvegicus arylacetamide deacetylase gene, complete cds 3746 8750 b13749 1 1.9E-01 P39768 SWISSPROT PAIR-RULE PROTEIN ODD-PAIRED 3886 8887 13886 3.37 1.9E-01 AB006784.1 NT Schizosaccharomyces pombe DNA for cytoplasmic dynein heavy chain, complete cds 3968 8967 13956 1. 47 1. 9E-01 AW754106. 1 EST HUMAN CM3-CT0315-271199-045-b11 CT0315 Homo sapiens cDNA Page 27 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Mot Similar Top Hit SEQ ID SEQ ID (Top) Hit Top Hit Acession Database Top Hit Descriptor NO: I NO : Signal BLAST E No. Source Value 4110 9104 14090 1 1.9E-01 BE834943.1 EST_HUMAN MR1-FN0010-290700-007-d04 FN0010 Homo sapiens cDNA 4851 9833 1. 11 1. 9E-01 AF223642. 1 NT Rattus norvegicus chemokine receptor CXCR3 mRNA, complete cds 32 5112 10098 1. 73 1. 8E-01 U73200. 1 NT Mus musculus p116Rip mRNA, complete cds 258 7717 10327 1. 97 1. 8E-01 AB022090. 1 NT Mus musculus Cctg gene for chaperonin containing TCP-1 gamma subunit, partial cds Homo sapiens calcium channel, voltage-dependent, beta 2 subunit (CACNB2) mRNA, and translated 369 5418 10433 1. 48 1. 8E-01 4502532 NT products 737 5760 10783 1. 61 1. 8E-01 AB021490. 2 NT Oryzias latipes gene for membrane guanyla cyclase OIGCI, complete cds 967 5982 11014 0. 75 1. 8E-01 AI912212. 1 EST_HUMAN wd71f02.x1 NIH_MGC_Lu24 Homo sapiens cDNA clone IMAGE : 2337051 3' 1075 6082 11112 1. 17 1. 8E-01 AF000580. 1 NT Dictyostelium discoideum plasmid Ddp5, complete genome 1269 6267 11309 7. 26 1. 8E-01 AL117189. 1 NT Yersinia pestis plasmid pCD1 1810 6800 1. 18 1. 8E-01 4505036 NT Homo sapiens latent transforming growth factor beta binding protein 4 (LTBP4) mRNA qg22d10. x5 NCl_CGAP_Kids Homo sapiens cDNA clone IMAGE : 1761811 3' similar to TR:O75936 O7593@ 1829 6819 1. 34 1. 8E-01 AI733708. 1 EST HUMAN GAMMA BUTYROBETAINE HYDROXYLASE ; Mus musculus Scya6, Scya9, Scya16-ps, Scya5 genes for small inducible cytokine A6 precursor, small 1871 6860 11948 1. 42 1. 8E-01 AB051897. 1 NT inducible cytokineA9 precursor, Scya16 pseudogene, small inducible cytokineA5 preeursor, complete cds 2620 7582 1. 63 1. 8E-01 AW935728. 1 EST HUMAN QV3-DT0018-081299-036-g04 DT0018 Homo sapiens cDNA 2827 7848 1. 6 1. 8E-01 AF184589. 1 NT Jonopsidium acaule LEAFY protein (LEAFY2) gene, partial cds 2833 7853 12873 1. 28 1. 8E-01 AW182300. 1 ESTHUMAN xj41a03. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2659756 3' 3051 8068 13077 2. 5 1. 8E-01 AW995178. 1 EST HUMAN QVO-BN0041-070300-147-c04 BN0041 Homo sapiens cDNA 3285 8296 13322 0. 68 1. 8E-01 BF183582. 1 EST_HUMAN 601809723R1 NIH_MGC_18 Homo sapiens cDNA clone IMAGE : 4040621 3' yj45e01. s1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 151704 3' similar to contains Alu 3540 8546 13553 0. 78 1. 8E-01 H03369. 1 EST HUMAN repetitive element ; yj45e01. s1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 151704 3'similar to contains Alu 3540 8546 13554 0. 78 1. 8E-01 H03369. 1 EST HUMAN repetitive element ; 4206 9199 1. 21 1. 8E-01 D37954. 1 NT Bovine NB25 mRNA for MHC class 11 (BoLA-DQB), complete cds 4424 9414 14401 5. 34 1. 8E-01 AL161556. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 56 Mus musculus Scya6, Scya9, Scya16-ps, Scya5 genes for small inducible cytokine A6 precursor, small 4627 9612 14601 3. 53 1. 8E-01 AB051897. 1 NT inducible cytokine A9 precursor, Scya16 pseudogene, small inducible cytokine A5 precursor, complete cds. 4661 9646 14634 1. 89 1. 8E-01 X92179. 1 NT S. tuberosum mRNA for alcohol dehydrogenase 4880 9859 14830 2. 79 1. 8E-01 AW814270. 1 EST HUMAN MR3-ST0203-151299-112-g06 ST0203 Homo sapiens cDNA 4924 9901 14877 6. 38 1. 8E-01 AF181258. 1 NT Mesocricetus auratus Na-taurocholate cotransporting polypeptide mRNA, partial cds 4942 9919 14899 1. 04 1. 8E-01 AI439881.1 EST_HUMAN ti57e04.x1 NCl_CGAP_Lym12 Homo sapiens cDNA clone IMAGE : 2134590 3' Page 28 of 209 Table 4 Singie Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession pabase Top Hit Descriptor ID NO : Signal BLAST E N Source. NO : NO : Value 5061 10030 14997 0. 93 1. 8E-01 X60206. 1 NT Escherichia cdi reverse transcriptase, retron EC86 5061 10030 14998 0. 93 1. 8E-01 X60206. 1 NT Escherichia cdi reverse transcriptase, retron EC86 572 5605 10603 1. 66 1. 7E-01 BE385164. 1 EST HUMAN 601274604F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3615768 5' 796 5817 10848 1. 92 1. 7E-01 X53330. 1 NT P. dumerilii histone gene cluster for core histones H2A, H2B, H3 and H4 946 5963 2 1. 7E-01 P35616 SW ISSPROT NEUROFILAMENT TRIPLET L PROTEIN (NEUROFILAMENT LIGHT POLYPEPTIDE) (NF-L) 1041 6051 11080 1. 31 1. 7E-01 AF081810. 1 NT Lymanfria dispar nucleopolyhedrovirus, complete genome 1Q41 6051 19089 1. 31 1. 7E-01 AF081810. 1 NT Lymantria dispar nucleopdyhedrovirus, complete genome 1781 6773 11865 1. 64 1. 7E-01 AL161573. 2 NT Aabidopsis thaliana DNA chromosome 4, contig fragment No. 69 1935 6921 1. 96 1. 7E-01 AF255051. 1 NT Homo sapiens BNIP3H (BNIP3H) gene, complete cds ; nuclear gene for mitochondrial product Vibrio cholerae hypoxanthine phosphoribosyltransferase (hpt) gene, partial cds, hemagglutininlproteasa 2788 7809 12826 2. 05 1. 7E-01 AF000716. 1 NT regulatory protein (hapR) gene, complete cds, and YRAL VIBCO gene, partial cds Vibrio cholerae hypoxanthine phosphoribosyltransferase (hpt) gene, partial cds, hemagglutininlprotease 27gg 7809 12827 2. 05 1. 7E-01 AF000716. 1 NT regulatory protein (hapR) gene, complete cds, and YRAL VIBCO gene, partia (cds 2856 7876 12892 1. 55 1. 7E-01 AA336909. 1 EST HUMAN EST41651 Endometrial tumor Homo sapiens cDNA 5'end 2928 7947 12964 1. 1 1. 7E-01 AJ238736. 1 NT Naja naja atra cbc 1 gene, exons 1-3 2928 7947 12965 1. 1 1. 7E-01 AJ238736. 1 NT Naja naja atra cbc 1 gene, exons 1-3 3032 8049 13058 1. 45 1. 7E-01 AF081514. 1 NT Taxus canadensis geranylgeranyl diphosphate synthase mRNA, complete cds Anabaena sp. ORF4 (partial), ORF3, ORF2, ORF1, adpA gene, adpB gene, adpC gene, adpD gene, adpE 3363 8371 13391 1. 56 1. 7E-01 AJ269505. 1 NT gene and adpF gene 3527 8533 13540 1. 17 1. 7E-01 AJ224877. 1 NT Homo sapiens hap1 gene, compiete CDS, Homo sapiens derivative 11 breakpoint fragment : partial intron 10 of the ALL-1IMLLIHRX gene fused to intron _ 3835 8837 13843 6. 17 1. 7E-01 AJ235377. 1 NT 5 of the AF-4IFEL gene 4428 9418 1. 89 1. 7E-01 X52936. 1 NT Schistocerca gegaria alpha repetitive DNA qh57e09. x1 Soares fetal liver spfeen 1 NFLS S1 Homo sapiens cDNA clone IMAGE : 1848808 3'similar to 4691 9676 14659 1. 31 1. 7E-01 AI247635. 1 EST HUMAN contains OFR. b1 OFR repetitive element ; 4932 9909 14886 8. 76 1. 7E-01 A8007941. 1 NT Homo sapiens mRNA for KIAA0472 protein, partial cds 126 5194 10208 1. 54 1. 6E-01 AF217532. 1 NT Homo sapiens mevalonate kinase gene, exon 6 and 7 671 7695 10705 1. 11 1. 6E-01 R31497. 1 EST HUMAN Yh5f12. r1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 135599 5' 1474 6471 11529 1. 07 1. 6E-01 AA548863. 1 EST HUMAN nk28d12. s1 NCI_CGAP Co11 Homo sapiens cDNA clone IMAGE : 1014839 3' 9490 6487 11542 2. 68 1. 6E-01 AF298117. 1 NT Homo sapiens homeobox protein OTX2 gene, complete cds 1883 6872 11961 1. 19 1. 6E-01 P22063 SW 15SPROT AXNIN-1 PRECURSOR (AXONAL GLYCOPROTEIN TAG-1) 1938 6924 1. 08 1. 6E-01 U10334. 1 NT Crassostrea gigas RNA polymerase II largest subunit mRNA, partial cds Page 29 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession TP Hit SEQ ID SEQ ID ID NO : Signal BLAST E No. Database NO : NO : Souce, Value 2322 7768 12418 0. 98 1. 6E-01 X94232. 1 NT H. sapiens mRNA for novel T-cell activation protein 2422 7393 12514 1. 22 1. 6E-01 AB037729. 1 NT Homo sapiens mRNA fa KIAA1308 protein, partial cds 2821 7842 12859 10. 97 1. 6E-01 AF185589. 1 NT Homo sapiens cytochrome P450 3A4 (CYP3A4) gene, promoter region 2821 7842 12860 10. 97 1. 6E-01 AF185589. 1 NT Homo sapiens cytochroma P450 3A4 (CYP3A4) gene, promoter region 3548 8555 13562 1. 16 1. 6E-01 AJ003165. 1 NT Populus trichocarpa cv. Trichobel ABI3 gene 3548 8555 13563 1. 16 1. 6E-01 AJ003165. 1 NT Populus trichocarpa cv. Trichobel ABI3 gene 3685 8689 13692 0. 73 1. 6E-01 AE000962. 1 NT Archaeoglobus fulgidus section 145 of 172 af the complete genome 3894 8894 2. 51 1. 6E-01 AE004413. 1 NT Vibrio cholerae chromosome II, secfion 70 of 93 of the complete chromosome 4202 9195 14177 10. 56 1. 6E-01 AF179680. 1 NT Homo sapiens apelin gene, complete cds 4334 9325 2. 61 1. 6E-01 AW 968601. 1 EST HUMAN EST380677 MAGE resequences, MAGJ Homo sapiens cDNA 4340 9331 3. 9 1. 6E-01 6753319 NT Mus musculus chaperonin subunit 3 (gamma) (Cct3), mRNA z184h09. s1 Stratagene colon (#1937204) Homo sapiens cDNA clone IMAGE : 511361 3'similar to TR : E221955 47gg g772 14755 1. 47 1. 6E-01 AA088343. 1 EST HUMAN E221955 38, 855 BP SEGMENT OF CHROMOSOME XIV. ; 4810 9794 14776 1. 19 1. 6E-01 AJ006356. 1 NT LyGOpersicon esculentum Rsal fragment 2, satellite region 4810 9794 14777 1. 19 1. 6E-01 AJ006356. 1 NT Lycopersicon esculentum Rsal fragment 2, satellite region LOW-DENSITY LIPOPROTEIN RECEPTOR-RELATED PROTEIN 2 PRECURSOR (MEGALIN) 5029 10000 14973 1. 21 1. 6E-01 P98158 SW ISSPROT (GLYCOPROTEIN 330) 246 5306 10316 1. 38 1. 5E-01 BE710087. 1 EST HUMAN IL3-HT0619-040700-197-E05 HT0619 Homo sapiens cDNA 246 5306 10317 1. 38 1. 5E-01 BE710087. 1 EST HUMAN IL3-HT0619-040700-197-E05 HT0619 Homo sapiens cDNA 582 7694 2. 11 1. 5E-01 AV711696. 1 EST HUMAN AV711696 DCA Homo sapiens cDNA clone DCAADH06 5' 774 5796 10824 1. 39 1. 5E-01 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 1080 6087 11116 1. 81 1. 5E-01 AJ251885. 1 NT Homo sapiens partial SLC22A2 gene for organic cafion transporter (OCT2), exon 1 1096 6103 2. 69 1. 5E-01 L36125. 1 NT Rattus norvegicus insulin-responsive glucose transporter (GLUT4) gene, 5'end 1197 6198 11234 0. 7 1. 5E-01 AW 195516. 1 EST HUMAN m39d11. x1 NCI CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2696085 3' 1256 6254 11295 2. 85 1. 5E-01 D26535. 1 NT Human gene for dihydrolipoamide succinyltransferase, complete cds (exon 1-15) 1256 6254 11296 2. 85 1. 5E-01 D26535. 1 NT Human gene for dihydrolipoamide succinyltransferase, complete cds (exon 1-15) 1451 6448 11509 1. 47 1. 5E-01 AF117340. 1 NT Mus musculus MAP kinase kinase kinase 1 (Mekk1) mRNA, complete cds 2639 7599 12713 1. 34 1. 5E-01 BF695381. 1 EST HUMAN 602083269F1 NIH MGC 81 Homo sapiens cDNA clone IMAGE : 4247537 5' xw56a02. C1 NCI CGAP Pan1 Homo sapiens cDNA clone IMAGE : 2831978 3'similar to gb : X55072_rna1 2843 7863 1. 06 1. 5E-01 AW 572516. 1 EST HUMAN THYROID HORMONE RECEPTOR ALPHA-1 (HUMAN) ; oo68d05. s1 NCI_CGAP GC4 Homo sapiens cDNA clone IMAGE : 1571337 3'similar to gb : M11433 3274 8286 13310 4. 64 1. 5E-01 AA935049. 1 EST HUMAN RETINOL-BINDING PROTEIN I, CELLULAR (HUMAN) ; 3670 8675 13678 2. 01 1. 5E-01 U09964. 1 NT Mus musculus ICR/Swiss glyceraldehyde 3-phosphate dehydrogenase (Gapd-S) gene, complete cds . Page 30 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ors (Top) Hit Top Hit Acession Database Top Hit Descriptor NO : NO : ID NO: Signal BLASTE No. Source Value Homo sapiens pyruvate dehydrogenase kinase, isoenzyme 1 (PDK1), nuclear gene encoding mitochondrial 3684 8688 13691 1. 04 1. 5E-01 7108358 NT protein, mRNA 3775 8778 13782 2. 39 1. 5E-01 AW665983. 1 EST_HUMAN hJ10f06.x1 Soares_NEL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2981411 3' 3937 8936 13929 0. 96 1. 5E-01 AW366659. 1 ESTHUMAN RC2-HT0149-191099-012-c09 HT0149 Homo sapiens cDNA 4059 9053 14040 8. 83 1. 5E-01 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 4585 9573 14563 1. 41 1.5E-01 BF687665. 1 EST_HUMAN 602067192F1 NIH_MGC_57 Homo sapiens cDNA clone IMAGE : 4066223 5' 4607 7599 12713 2. 83 1. 5E-01 BF695381. 1 EST HUMAN 602083269F1 NIH MGC 81 Homo sapiens cDNA clone IMAGE : 4247537 5' 4840 9824 14799 1. 66 1. 5E-01 AL161560. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 60 297 5354 1. 51 1. 4E-01 AF009663. 1 NT Homo sapiens T cell receptor beta locus, TCRBV8S5P to TCRBV21S2A2 region 899 5917 2. 72 1. 4E-01 D78638. 1 NT Xenopus laevis mRNA for DNA (cytosine-5-)-methyltransferase, complete cds 1240 6238 2. 11 1. 4E-01 T91864. 1 EST_HUMAN yd54c01.s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 112032 3' 1709 6704 1. 2 1. 4E-01 6679980 NT Mus musculus growth differentiation factor 5 (Gdf5), mRNA 1712 6707 11783 1. 61 1. 4E-01 AE001710. i NT Thermotoga maritima section 22 of 136 of the complete genome 1939 6925 8. 74 1. 4E-01 AA720615. 1 EST_HUMAN ny72d07.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE : 1283821 3' 2720 7677 12791 4. 16 1. 4E-01 AI933496. 1 EST HUMAN wm74d01. x1 NCI CGAP Ut2 Homo sapiens cDNA clone IMAGE : 2441665 3' 3464 8472 1. 55 1. 4E-01 BF341524. 1 EST_HUMAN 602013527F1NCI_CGAP_Brn64 Homo sapiens cDNA clone IMAGE : 4149126 5' 3803 8806 13811 1. 17 1. 4E-01 R59232. 1 EST_HUMAN yg97a03.r1 Soares Infant brain 1NIB Homo sapiens cDNA clone IMAGE : 41467 5' 3803 8806 13812 1. 17 1. 4E-01 R59232. 1 EST HUMAN yg97a03. r1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 41467 5' 4050 9046 14034 11. 16 1. 4E-01 A1699094. 1 EST_HUMAN tx56c02.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 2273570 3' 4050 9046 14035 11.16 1.4E-01 A1699094. 1 EST_HUMAN tx56c02.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 2273570 3' 4115 9109 14094 3. 6 1. 4E-01 AE001710. 1 NT Thermotoga maritima section 22 of 136 of the complete genome 320 5375 10384 2. 28 1. 3E-01 4758467 NT Homo sapiens G protein-coupled receptor 50 (GPR50) mRNA 320 5375 10385 2. 28 1. 3E-01 4758467 NT Homo sapiens G protein-coupled receptor 50 (GPR50) mRNA 525 5560 10563 1.88 1.3E-01 AB013139. 1 NT Homo sapiens gene for NBS1, complete cds 630 5658 10663 1. 51 1. 3E-01 AJ277606. 1 NT Human calicivirus HU/NLV/Girlingtonl93/UK RNA for capsid protein (ORF2), strain HU/NLV/Girlington/93/UK 630 5658 10664 1. 51 1. 3E-01 AJ277606. 1 NT Human calicivirus HU/NLV/Girlington/93/UK RNA for capsid protein (ORF2), strain HU/NLV/Girlington/93/UK 834 5853 10893 0. 83 1. 3E-01 X53330. 1 NT P. dumerilii histone gene cluster for core histones H2A, H2B, H3 and H4 883 5901 10942 1. 49 1. 3E-01 AF139518. 1 NT Rattus norvegicus A-kinase anchor protein mRNA, complete cds 1010 6020 11049 1. 51 1. 3E-01 AL117078. 1 NT Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation 1110 61162. 671. 3E-01 AL115265. 1 NT Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation 1196 6197 11233 1. 71 1. 3E-01 AV712467. 1 EST HUMAN AV712467 DCA Homo sapiens cDNA clone DCAAFF05 5' 1418 6415 0. 91 1. 3E-01 AF146277. 1 NT Homo sapiens adapter protein CMS mRNA, complete cds Page 31 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Top Hit Descriptor NO: NO: Source Value 1919 6905 11999 1.66 1.3E-01 AL117078.1 NT Botrytis cinerea strain T4 cDNA library under conditions of nitrogen deprivation Rhodopseudomonas acidophila pucB5, pucA5, pucB6, pucA6, pucB7, pucA7, pucB8, pucA8 and pucC 2106 7086 0. 97 1. 3E-01 AJ243578. 1 NT genes and ORF151 2228 7205 1. 04 1. 3E-01 AW812104. 1 EST_HUMAN RC4-ST0173-191099-032-d12 ST0173 Homo sapiens cDNA 2318 7293 2. 79 1. 3E-01 AE001016. 1 NT Archaeoglobus fulgidus section 91 of 172 of the complete genome 2510 7478 12593 1. 78 1. 3E-01 M86918. 1 NT Carassius auratus keratin type I mRNA, complete cds Homo sapiens transcription factor IGHM enhancer 3, JM11 protein, JM4 protein, JM5 protein, T54 protein, JM10 protein, A4 differentiation-dependent protein, triple LIM domain protein 6, and synaptophysin genes, 3278 8290 13315 0. 98 1. 3E-01 AF196779. 1 NT complete cds ; and L-type calcium channel a' 3368 8376 13396 1. 03 1. 3E-01 M21572. 1 NT Bovine branched chain alpha-keto acid dihydrolipoyi transacylase mRNA, complete cds 3639 8645 13651 0. 81 1. 3E-01 AP000001. 1 NT Pyrococcus horikoshii OT3 genomic DNA, 1-287000 nt. position (1/7) 3639 8645 13652 0. 81 1. 3E-01 AP000001. 1 NT Pyrococcus horikoshii OT3 genomic DNA, 1-287000 nt. position (1/7) 3882 8883 1. 37 1. 3E-01 AL161581. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 77 3938 5658 10663 1. 73 1. 3E-01 AJ277606. 1 NT Human calicivirus HU/NLV/Girlington/93/UK RNA for capsid protein (ORF2), strain HU/NLV/Girlington/93/UK 3938 5658 10664 1. 73 1. 3E-01 AJ277606. 1 NT Human calicivirus HU/NLV/Girlington/93/UK RNA for capsid protein (ORF2), strain HU/NLV/Girlington/93/UK 4016 9012 0. 82 1. 3E-01 AF020713. 1 NT Bacteriophage SPBc2 complete genome 4034 9030 4. 19 1. 3E-01 AW364341. 1 ESTHUMAN QV3-DT0018-081299-036-a03DT0018 Homo sapiens cDNA 4042 9038 14028 1. 79 1. 3E-01 AF026805. 1 NT Schistosoma mansoni fructose bisphosphate aldolase mRNA, complete cds 4061 9055 14042 18. 31 1. 3E-01 AW273741. 1 EST HUMAN xv23f10. x1 Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE : 2813995 3' 4187 9180 1. 36 1. 3E-01 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21 C080 4403 9394 14378 2. 77 1. 3E-01 BE272339. 1 EST HUMAN 601126096F1 NIH MGC 9 Homo sapiens cDNA clone IMAGE : 2990063 5' 4940 9917 14897 1. 76 1. 3E-01 AI432531. 1 EST_HUMAN th38c10. x1 NCI CGAP_Pan1 Homo sapiens cDNA clone IMAGE : 2120562 3' tf39b02.x1 NCI_CGAP_Bm23 Homo sapiens cDNA clone IMAGE : 2098539 3'similar to gb : U05760_rna1 382 5457 10474 9. 01 1. 2E-01 AI421744. 1 EST HUMAN ANNEXIN V (HUMAN) ; 421 5079 1. 74 1. 2E-01 U66912. 1 NT Dictyostelium discoideum ORF DG1016 gene, partial cds 543 5577 2. 9 1. 2E-01 AF039442. 1 NT Homo sapiens colon cancer antigen NY-CO-45 mRNA, partial cds 1358 6355 11405 2.5 1. 2E-01 AU149146. 1 EST HUMAN AU149146 NT2RM4 Homo sapiens cDNA clone NT2RM4001691 3' 1358 6355 11406 2. 5 1. 2E-01 AU149146. 1 ESTHUMANAU149146NT2RM4 Homo sapiens cDNA clone NT2RM4001691 3' 1364 6361 3. 56 1. 2E-01 AV735249. 1 EST_HUMAN AV735249 cdA Homo sapiens cDNA clone cdAAJB11 5' al48e09.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 1460584 3'similar to TR : Q16671 1477 6474 1. 03 1. 2E-01 AA897474. 1 EST HUMAN Q16671 ANTI-MULLERIAN HORMONE TYPE 11 RECEPTOR PRECURSOR. ; Page 32 of 209 Table 4 Sinale Exon PrnhPS Fxnrascai in NRI 1f1 (1 C. allc Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID DAtabase Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value NUCLEAR FACTOR OF ACTIVATED T-CELLS, CYTOPLASMIC 4 (T CELL TRANSCRIPTION FACTOR 1591 6587 11649 1. 16 1. 2E-01 Q14934 SWISSPROT NFAT3) (NF-ATC4) (NF-AT3) 1611 6607 11671 2. 24 1. 2E-01 Al285402. 1 EST HUMAN qt69f09. x1 NCI CGAP_Eso2 Homo sapiens cDNA clone IMAGE : 1960553 3' 1731 6726 9. 87 1. 2E-01 X89211. 1 NT H. sapiens DNA for endogenous retroviral like element 1876 6865 3. 06 1. 2E-01 AW449368. 1 EST_HUMAN HI-H-Bl3-aki-e-10.0-Ul.s1 NCI_CGAP_Sub5 Homo sapiens cDNA clone IMAGE : 2734554 3' 2118 7098 12211 2. 31 1. 2E-01 BF248490. 1 EST_HUMAN I601821567F1 NIH MGC 62 Homo sapiens cDNA clone IMAGE : 4046224 5' 2514 7482 12598 1. 4 1. 2E-01 AW996556. 1 ESTHUMAN QV3-BN0046-220300-129-f10 BN0046 Homo sapiens cDNA 2769 7790 12812 1. 4 1. 2E-01 U18018. 1 NT Human E1A enhancer binding protein (E1A-F) mRNA, partial cds as80c09. x1 Barstead colon HPLRB7 Homo sapiens cDNA clone IMAGE : 2335024 3'similar to gb : L05095 2832 7852 12872 2. 31 1. 2E-01 AI720470. 1 EST HUMAN 60S RIBOSOMAL PROTEIN L30 (HUMAN) ; 2864 7884 12904 3. 25 1. 2E-01 M16364. 1 NT Human creatine kinase-B mRNA, complete cds 2936 7599 12973 0.9 1.2E-01 X56882.1 NT Wheat mRNA for a group 3 late embryogenesis abundant protein (LEA) 3159 8175 13198 1. 63 1. 2E-01 AW370668. 1 EST HUMAN QV1-BT0259-261099-021-d05 BT0259 Homo sapiens cDNA 3183 8199 0. 79 1. 2E-01 U67600. 1 NT Methanococcus jannaschii section 142 of 150 of the complete genome 3442 8450 13476 0. 67 1. 2E-01 X56882. 1 NT Wheat mRNA for a group 3 late embryogenesis abundant protein (LEA) i 3442 8450 13477 0. 67 1. 2E-01 X56882. 1 NT Wheat mRNA for a group 3 late embryogenesis abundant protein (LEA) 3525 8407 0. 71 1. 2E-01 Z99118. 1 NT Bacillus subtilis complete genome (section 15 of 21) : from 2795131 to 3013540 4058 9052 14038 1. 91 1. 2E-01 Z54255. 1 NT P. clarkii mRNA ; repeat region (ID 2MRT7) I 4058 9052 14039 1. 91 1. 2E-01 Z54255. 1 NT P. clarkii mRNA ; repeat region (ID 2MRT7) Rana ridibunda pituitary adenytate cyciase-activating potypeptide variant 2 precursor, mRNA, complete cds, 4648 9633 0. 92 1. 2E-01 AF221633. 1 NT alternatively spliced 4857 9838 14811 9. 45 1. 2E-01 BF577357. 1 EST HUMAN 602135185F1 NIH MGC 81 Homo sapiens cDNA clone IMAGE : 4290165 5' 4937 9914 14892 3. 98 1. 2E-01 Al163227. 2 NT Homo sapiens chromosome 21 segment HS21C027 4937 9914 14893 3. 98 1. 2E-01 AL163227. 2 NT Homo sapiens chromosome 21 segment HS21 C027 5057 10027 4. 85 1. 2E-01 Q57599 SWISSPROT RIBONUCLEASE HII (RNASE Hil) 560 5594 10594 0. 74 1. 1E-01 Al561003. 1 EST_HUMAN tn18d08.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE : 2167983 3' nm08g11.s1 NCI_CGAP_Co10 Homo sapiens cDNA clone IMAGE : 1059620 3'similar to gb : X06985rna1 611 5638 10604 6. 71 1. 1 E-01 AA569006. 1 EST HUMAN HEME OXYGENASE 1 (HUMAN) ; 1037 6047 11077 1. 16 1. 1E-01 BF697308. 1 EST_HUMAN 602129847F1 NIH_MGC_56 Homo sapiens cDNA clone IMAGE : 4286771 5' 1069 6077 1. 46 1. 1 E-01 AL161560. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 60 1141 7740 111177 3. 26 1. 1E-01 AW972158. 1 EST HUMAN EST384142 MAGE resequences, MAGL Homo sapiens cDNA 1230 6229 11274 1. 76 1. 1E-01 D64004. 1 NT Synechocyslis sp. PCC6803 complete genome, 23/27, 2868767-3002965 1488 6485 11540 1. 79 1. 1E-01 AU140363. 1 EST HUMAN AU140363 PLACE2 Homo sapiens cDNA clone PLACE2000403 5' 2250 7227 2. 31 1. 1 E-01 6755215 NT Mus musculus pre T-ceil antigen receptor alpha (Ptcra), mRNA Page 33 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top huit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor Value 2468 7707 1.24 1.1E-01 6978676 NT Rattus norvejious Procollagen 11 alpha 1 (Col2a1), mRNA 2782 7803 12820 1.07 1.1E-01 S82418.1 NT Interleukin-12 p35 subunit mice, Genomic, 700 nt, segment 4 of 5] 2963 7981 12995 0. 83 1. 1E-01 F03265. 1 EST HUMAN HSC1RF022 normalized infant brain cDNA Homo sapiens cDNA clone c-1 rf02 3' 3267 8280 1. 57 1. 1E-01 6753231 NT Mus musculus calcium channel, voltage-dependent, T type, alpha 1G subunit (Cacna1g), mRNA 3336 8346 13364 2.75 1.1E-01 BE393186.1 EST_HUMAN 601308679F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3627066 5' 3369 8377 13397 1.5 1.1E-01 X62135. 1 NT C. reinhardtii nuclear gene on linkage group XIX yq62g08. s1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 200414 3'similar to contains 3411 8420 13449 1. 26 1. 1E-01 R96946. 1 EST HUMAN Alu repetitive element ; 3501 8509 135230. 81. 1E-01 Y07695. 1 NT A. immersus gene for transposase 3619 8626 0.84 1.1E-01 P97384 SWISSPROT ANNEXIN XI (CALCYCLIN-ASSOCIATED ANNEXIN 50) (CAP-50) 3627 8634 13639 1. 44 1.1E-01 X52708. 1 NT G. gallus gene encoding non-histone chromosomal protein HMG-14b, exons 4 and 5 3994 8991 13975 1.01 1.1E-01 AW819412. 1 EST_HUMAN MR3-ST0290-290100-025-g07 ST0290 Homo sapiens cDNA 3994 8991 139761. 011. 1E-01 AW819412. 1 EST_HUMAN MR3-ST0290-290100-025-g07 ST0290 Homo sapiens cDNA 4131 9126 9. 98 1. 1E-01 AF157066. 1 NT Drosophila melanogaster klarsicht protein (klar) mRNA, complete cds Tapa-1=integral membrane protein TAPA-1 [mice, B cell lymphom line 38C13, Genomic, 1973 nt, segment 1 4505 9495 14473 0. 96 1. 1E-01 S44957. 1 NT of 7] 4685 9670 146521. 091. 1E-01 Y07695. 1 NT A. immersus gene for transposase 4856 9837 0. 86 1. 1E-01 AW026547. 1 EST_HUMAN wv14h02.x1 NCI_CGAP_Brn23 Homo sapiens cDNA clone IMAGE : 2529555 3' 5002 9973 1 1.1E-01 8923317 NT Homo sapiens hypothetical protein FLJ20342 (FLJ20342), mRNA 1182 6184 3. 86 1.0E-01 O62855 SWISSPROT DEOXYRIBONCULEASE II PRECURSOR (DNASE ii) (ACID DNASE) (LYSOSOMAL DNASE tt) ws08d01.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2496577 3'similar to contains MER7. t3 1253 6251 11292 1.81 1.0E-01 Al985499. 1 EST HUMAN MER7 repetitive element ; 1369 6366 11415 1.69 1.0E-01 AL161504. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 16 3434 8442 13468 0. 99 1. OE-01 BF033991. 1 EST_HUMAN 601456301F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3859849 5' 3637 8643 13649 0. 87 1. OE-01 BF239818. 1 EST_HUMAN 601906489F1 NIH_MGC_54 Homo sapiens cDNA clone IMAGE : 4134071 5' 3848 8850 13858 2. 47 1. OE-01 BF365703. 1 ESTHUMAN QV2-NT0048-160800-316-e05NT0048 Homo sapiens cDNA I 4283 9275 14263 1. 88 1. OE-01 AE002265. 2 NT Chlamydophila pneumoniae AR39, section 91 of 94 of the complete genome 4423 9413 1. 85 1.0E-01 A1792349. 1 EST HUMAN an32c04. y5 Gessler Wilms tumor Homo sapiens cDNA clone IMAGE : 1700358 5' 4574 9562 14550 1. 74 1. OE-01 U50450. 1 NT Drosophila melanogaster tyrosine kinase p45 isaform (fer) mRNA, complete cds 4767 9751 14740 2. 04 1. 0E-01 AW952344. 1 EST HUMAN EST364414 MAGE resequences, MAGB Homo sapiens cDNA 4967 9944 14921 0.93 1.0E-01 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21 C047 5022 9993 3.42 1.0E-01 BE881566. 1 EST HUMAN 601490280F1 NIH_MGC 69 Homo sapiens cDNA clone IMAGE : 3892842 5' 2714 7671 12784 1. 21 9.9E-0.2 BE545554. 1 EST HUMAN 601070219F1 NIH_MGC 12 Homo sapiens cDNA clone IMAGE : 3456365 5' 2714 7671 12785 1. 21 9. 9E-02 BE545554. 1 EST HUMAN 601070219F1 NIH MGC 12 Homo sapiens cDNA clone IMAGE : 3456365 5' Page 34 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Top Hit Descriptor NO: NO: Source Value 3192 8208 13229 1.36 9.3E-02 AF099810.1 NT Homo eapiens neurexin III-alpha gene, partial cds 559 5593 1.41 9.8E-02 X56338. 1 INT O.sativa RAmy3C gene for alpha-amylase 3072 8088 13101 4.03 9.8E-02 AF184274. 1 NT Daucus carota leucoanthocyanidin dioxygenase 2 (LDOX) mRNA, LDOX-2 allele, complete cds 4102 9096 14081 5. 22 9. 8E-02 AF257329. 1 NT Leptosphaeria maculans beta-tubulin mRNA, complete cds 4102 9096 14082 5. 22 9. 8E-02 AF257329. 1 NT Leptosphaeria maculans beta-tubulin mRNA, complete cds 13326330 11379 1. 38 9. 7E-02 AB005808. 1 NT Aloe arborescens mRNA for NADP-malic enzyme, complete cds 1550 6547 1. 11 9. 7E-02 4503710 NT Homo sapiens fibroblast growth factor receptor 3 (achondroplasia, thanatophoric dwarfism) (FGFR3) mRNA 2199 7177 12300 1. 44 9. 7E-02 BE168660. 1 EST_HUMAN QV1-HT0516-070300-095-a04 HT0516 Homo sapiens cDNA 3875 8876 3.83 9. 7E-02 Q99795 SWISSPROT CELL SURFACE A33 ANTIGEN PRECURSOR (GLYCOPROTEIN A33) 1966 6951 12054 0. 92 9. 6E-02 Al080721. 1 EST_HUMAN oz47d11.x1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE : 1678485 3' 19666951 12055 0. 92 9.6E-0.2 Al080721. 1 ESTHUMAN oz47d11. x1 SoaresNhHMPuS1 Homo sapiens cDNA clone IMAGE : 1678485 3' 4219 9212 14191 5. 54 9. 6E-02 Z32686. 2 NT Proteus mirabilis fimbrial operon, strain Hl4320 4842 9825 14800 1. 16 9. 6E-02 AW966230. 1 EST_HUMAN ESt378303 MAGE resequences, MAGI Homo sapiens cDNA 4972 9948 14926 0. 97 9. 6E-02 BE061729. 1 EST HUMAN RC5-BT0254-031099-011-a03 BT0254 Homo sapiens cDNA 3985 8983 13969 2. 27 9. 5E-02 AW992395. 1 EST HUMAN CM2-BN0023-050200-087-f12 BN0023 Homo sapiens cDNA 4947 9924 14902 0. 93 9. 5E-02 U63374. 1 NT Lycopersicon esculentum poygalacturonase isoenzyme 1 beta subunit gene, complete cds 1797 6788 11.822 2. 69 9.4E-02 BF671063. 1 EST_HUMAN 602150882F1 NIH_MGC_81 Homo sapiens cDNA clone IMAGE : 4291917 5' 3788 8791 13796 5.14 9. 4E-02 Z33059. 1 NT M. capricolum DNA for CONTIG MC073 2920 7939 1.7 9. 3E-02 4809280 NT Homo sapiens BAl1-associated protein 3 (BAIAP3) mRNA 2959 7978 6. 91 9. 3E-02 6912525 NT Homo sapiens nasopharyngeal epithelium specific protein 1 (NESG1), mRNA 3182 8198 13222 2.33 9. 3E-02 BF575511. 1 EST HUMAN 602133086F1 NIH MGC 81 Homo sapiens cDNA clone IMAGE : 4288269 5' 4029 9025 14012 3. 03 9. 3E-02 BE391943. 1 EST_HUMAN 601286082F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3607653 5' 40299025 140133. 039. 3E-02 BE391943. 1 EST_HUMAN 601286082F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3607653 5' 4595 9583 2.31 9. 3E-02 AV732224. 1 EST HUMAN AV732224 HTF Homo sapiens cDNA clone HTFAUA06 5' 228 5291 10297 7. 76 9. 2E-02 U60315. 1 NT Molluscum contagiosum virus subtype 1, complete genome 228 5291 10298 7. 76 9.2E-02 U60315. 1 NT Molluscum contagiosum virus subtype 1, complete genome 228 5291 10299 7. 76 9. 2E-02 U60315. 1 NT Molluscum contagiosum virus subtype 1, complete genome 2166 7145 3.57 9. 2E-02 R54156. 1 EST HUMAN yg98f07. r1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 41618 5' 3106 8122 13140 3. 95 9. 2E-02 Q28631 SWISSPROT MAJOR EPIDIDYMIS-SPECIFIC PROTEIN E4 (EPIDIDYMAL PROTEIN BE-20) 3233 8248 13269 0. 82 9.2E-02 AA534354. 1 EST_HUMAN nf79e01.s1 NCI_CGAP_Co3 Homo sapiens cDNA clone IMAGE : 926136 3' 3505 8513 1. 06 9. 2E-02 6755215 NT Mus musculus pre T-cell antigen receptor alpha (Ptcra), mRNA 4116 9110 0. 98 9. 2E-02 U92048. 1 NT Human herpesvirus 1 strain KOS-63, latency-associated transcript, promoter region 4181 9174 0. 72 9. 2E-02 BE299722. 1 EST HUMAN 600944365F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 2960176 5' Page 35 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top HIt Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value 4504 9494 14472 1.99 9.2E-02 X96402.1 NT G.gallus Mia-CK gene 420 5078 10063 6. 08 9. 1E-02 X77665. 1 NT O. cuniculus k12 keratin gene 3585 8592 0. 93 9. 1E-02 AW372569. 1 EST HUMAN PM2-BT0349-161299-001-f02 BT0349 Homo sapiens cDNA 4357 9348 14328 1. 84 9. 1E-02 AL161554. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 54 FOLATE RECEPTOR ALPHA PRECURSOR (FR-ALPHA) (FOLATE RECEPTOR 1) (FOLATE RECEPTOR, ADULT) (ADULT FOLATE-BINDING PROTEIN) (FBP) (OVARIAN TUMOR-ASSOCIATED 735 5758 10780 4. 53 9. 0E-02 P15328 SWlSSPROT ANTIGEN MOV18) (KB CELLS FBP) hv39g10.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3175842 3'similar to contains Alu 1594 6590 11651 5. 3 9. 0E-02 BE220482. 1 EST HUMAN repetitive element ; 2731 7688 12802 2. 97 9. OE-02 AF138522. 1 NT HIV-1 p8c095-06 from USA envelope glycoprotein (env) gene, partial cds 2731 7688 12803 2. 97 9. 0E-02 AF138522. 1 NT HIV-1 p8c095-06 from USA envelope glycoprotein (env) gene, partial cds 3262 8275 13299 0. 76 9. 0E-02 AF279135. 1 NT Dictyostelium discoideum spore coat structural protein SP65 (cotE) gene, complete cds 4175 9169 14157 0. 87 9. 0E-02 S68757. 1 NT corticosteroid-binding globulin [Saimiri sciureus=squirrel monkeys, liver, mRNA, 1474 nt] 4175 9169 14158 0. 87 9. 0E-02 S68757. 1 NT corticosteroid-binding globulin [Saimiri sciureus=squirrel monkeys, liver, mRNA, 1474 nt] 4294 9286 14274 0. 91 9. 0E-02 P55268 SWISSPROT LAMININ BETA-2 CHAIN PRECURSOR (S-LAMININ) 4533 9523 14510 2. 08 9. 0E-02 X65740. 2 NT Plasmodium falciparum P-type ATPase 3 gene 5028 9999 14972 1. 01 9. 0E-02 Q24597 SWISSPROT REGULATORY PROTEIN ZESTE 1411 6409 11467 1.4 8. 9E-02 BF701593. 1 EST_HUMAN 602129030F2 NIH_MGC_56 Homo sapiens cDNA clone IMAGE : 4285951 5' 1411 6409 11468 1. 4 8. 9E-02 BF701593. 1 EST HUMAN 602129030F2 NIH MGC 56 Homo sapiens cDNA clone IMAGE : 4285951 5' 4076 9070 2. 28 8. 9E-02 AF286055. 1 NT Atrichum angustatum AtranFlo2 protein (AtranFlo2) gene, partial cds 1355 6352 11402 1. 5 8. 8E-02 Q27474 SWISSPROT PROBABLE DNA LIGASE (POLYDEOXYRIBONUCLEOTIDE SYNTHASE [ATP]) 3807 8810 13816 1. 16 8. 8E-02 AA299128. 1 EST HUMAN EST11595 Uterus Homo sapiens cDNA 5'end TRANSCRIPTION INITIATION FACTOR TFIID 135 KDA SUBUNIT (TAFII-135) (TAFI1135) (TAFII-130) 3924 8924 3. 83 8. 8E-02 000268 SWISSPROT (TAF11130) 1608 6604 116671. 11 8. 7E-02 Al167281. 1 ESTHUMAN ox65b01. s1 SoaresNhHMPuS1 Homo sapiens cDNA clone IMAGE : 1661161 3' Homo sapiens zinc finger protein 92 (ZFP92), expressed-Xq28STS protein (XQ280RF), and biglycan (BGN) 3610 8617 13626 4. 63 8. 7E-02 U82695. 2 NT genes, complete cds ; and plasma membrane calcium ATPase isoform 3 (PMCA3) gene, partial cds Homo sapiens zinc finger protein 92 (ZFP92), expressed-Xq28STS protein (XQ280RF), and biglycan (BGN) 3610 8617 13627 4. 63 8. 7E-02 U82695. 2 NT genes, complete cds ; and plasma membrane calcium ATPase isoform 3 (PMCA3) gene, partial cds 4568 9556 14544 1. 24 8. 7E-02 AF178636. 1 NT Mus musculus JNK interacting protein-3a (Jip3) mRNA, complete cds Methanobacterium thermoautotrophicum from bases 1176181 to 1189406 (section 101 of 148) of the 4929 9906 1. 08 8. 7E-02 AE000895. 1 NT complete genome Page 36 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top nit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Value 1233 6231 11275 6. 22 8. 6E-02 AJ271736. 1 NT Homo sapiens Xq pseudoautosomat region ; segment 2/2 2182 7161 12281 1. 3 8.6E-02 BE408667. 1 EST_HUMAN 601304016F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3638643 5' 3114 8130 13149 3. 35 8. 6E-02 L05468. 1 NT Trichomonas vaginalis beta-tubulin (but) gene, complete cds 3559 8566 3.97 8.6E-02 AF153362. 1 NT Dictyostelium discoideum adenyy cyclase (acrA) gene, complete cds 2333 7307 12428 1.85 8.5E-0.2 AE000652. 1 NT Helicobacter pytori 26695 section 130 of 134 of the complete genome 2596 7776 12674 3. 69 8.4E-02 W69330. 1 EST HUMAN zd44e11. r1 Soares fetal heart NbHH19W Homo sapiens cDNA clone IMAGE : 343532 5' 4228 9222 14202 0. 99 8.4E-02 AF257213. 1 NT Cavia porcellus glycoprotein alpha-subunit mRNA, complete cds 4228 9222 14203 0. 99 8.4E-02 AF257213. 1 NT Cavia porcellus glycoprotein alpha-subunit mRNA, complete cds 3512 8520 13532 5. 85 8. 3E-02 P75334 SWISSPROT HYPOTHETICAL LIPOPROTEIN MG309 HOMOLOG PRECURSOR 4559 9547 14532 0.83 8.3E-02 AB038490.1 NT Homo sapiens gene for fukutin, complete cds 1360 6357 6. 17 8. 2E-02 Y08170. 2 NT Gallus gallus mRNA for for OBCAM protein gamma isoform 1465 6462 11521 2.51 8. 2E-02 AF167077. 2 NT Canis familiaris glutamate transporter (EAAT4) mRNA, complete cds 3000 8018 2. 44 8. 2E-02 AL163206. 2 NT Homo sapiens chromosome 21 segment HS21C006 3713 8717 1. 37 8. 2E-02 AL161498. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 10 3901 8091 13899 1.16 8.2E-02 AL163206.2 NT Homo sapiens chromosome 21 segment HS21 C006 4161 9156 14139 5. 75 8.2E-02 P48960 SWISSPROT LEUCOCYTE ANTIGEN CD97 PRECURSOR 4161 9156 14140 5.75 8.2E-02 P48960 SWISSPROT LEUCOCYTE ANTIGEN CD97 PRECURSOR 4161 9156 14141 5.75 8.2E-02 P48960 SWISSPROT LEUCOCYTE ANTIGEN CD97 PRECURSOR 4909 9888 14861 3. 34 8. 2E-02 U76009. 1 NT Mus musculus zinc transporter (ZnT-3) gene, complete cds 5027 999814971 1. 29 8.2E-02 AU119830. 1 EST HUMAN AU119830 HEMBA1 Homo sapiens cDNA clone HEMBA1006744 5' Pseudomonas putida malonate decarboxytase gene cluster (mdcA, mdcB, mdcC, mdcD, mdcE, mdcG, 1464 6461 11520 1. 06 8.1E-02 Ab017138.1 NT mdcH, mdcL. and mdcM genes), complete cds 5062 1003114999 1*058. 1E-02 AE004673. 1 NT Pseudomonas aeruginosa PA01, section 234 of 529 of the complete genome I 6 7711 10072 4. 12 8.0E-02 AW954653. 1 EST HUMAN EST366723 MAGE resequences, MAGC Homo sapiens cDNA 1659 7752 11728 9. 298. 0E-02 D26535. 1 NT Human gene for dihydrolipoamide succinyitransferase, complete cds (exon 1-15) 1659 7752 1172 9. 29 0.8E-02 D26535. 1 NT Human gene for dihydrolipoamide succinyltransferase, complete cds (exon 1-15) 1862 6851 11939 2. 81 8. OE-02 BE067219. 1 EST HUMAN PM3-BT0347-170200-001-b08 BT0347 Homo sapiens cDNA 2400 7371 2.75 8.0E-02 BF246744. 1 EST_HUMAN 601855548F1 NIH_MGC_57 Homo sapiens cDNA clone IMAGE : 4075619 5' 2830 7850 12869 0. 67 8.0E-02 AL445067.1 NT Thermoplasma acidophilum complete genome; segment 5/5 3726 8730 13728 0. 73 8.0E-02 AW966118.1 EST_HUMAN EST378191 MAGE resequences, MAGI Homo sapiens cDNA i 3958 8956 0.76 8.0E-02 4503034 NT Homo sapiens cAMP responsive element binding protein-like 2 (CREBL2) mRNA 4631 9616 14607 1.25 8.0E-02 AI434202. 1 EST HUMAN ti31g02. x1 NCI_CGAP Gas4 Homo sapiens cDNA clone IMAGE : 2132114 3' 4669 9654 7.08 8.0E-02 X72794. 1 NT M.musculus gene for gelatinase B 5060 5939 10971 0.67 8.0E-02 U60315.1 NT Molluscum contagiosum virus subtype 1, complete genome Page 37 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Eression (Top) Hit Top Hit Acession Top Hit Descriptor ID NO : Signal BLAST E No. Database NO : NO : Source, Value 2109 7089 12203 2. 54 7. 9E-02 BE250008. 1 EST HUMAN 600943191F1 NIH_MGC_15 Homo sapiens cDNA clone IMAGE : 2959510 5' ar98c08. x1 Barstead colon HPLRB7 Homo sapiens cDNA cione IMAGE : 2173646 3'similar to gb : Z26876 2910 7929 12948 11. 99 7. 9E-02 AI582029. 1 EST HUMAN 60S RIBOSOMAL PROTEIN L38 (HUMAN) ; 3760 8763 13763 5. 05 7. 9E-02 6681044 NT Mus musculus colony stimulating factor 1 receptor (Csf1 r), mRNA 3760 8763 13764 5. 05 7. 9E-02 6681044 NT Mus musculus colony stimulaHng factor 1 receptor (Csf1 ), mRNA 4666 9651 1. 4 7. 9E-02 AB008019. 1 NT Arabidopsis thaliana RXW 24L mRNA, partial cds . oo59d02. y5 NCI CGAP Lu5 Homo NCl_CGAP_Lu5 cDNA clone IMAGE sapiens 1570467 5'similar IMAGE contains L1. 5'similar L1 1191 6192 11228 1. 59 7. 8E-02 AI793275. 1 EST HUMAN repe64ve element ; oo59d02. y5 NCI CGAP Lu5 Homo sapiens cDNA clone IMAGE : 1570467 5'similar to contains L1. t3 L1 1191 6192 11229 1. 59 7. 8E-02 A1793275. 1 EST HUMAN repetitive element ; 2332 7306 12426 1 7. 8E-02 AF221942. 1 NT Sus scrofa telomerase RNA pseudogena 2332 7306 12427 1 7. SE-02 AF221942. 1 NT Sus scrofa telomerase RNA pseudogene 3663 8668 1. 25 7. 8E-02 BE250048. 1 EST HUMAN 600943055F1 NIH MGC 15 Homo sapiens cDNA clone IMAGE : 2959693 5' 4914 8668 3. 15 7. 8E-02 BE250048. 1 EST HUMAN 600943055F1 NIH MGC 15 Homo sapiens cDNA clone IMAGE : 2959693 5' tg48g12. x1 Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE : 2112070 3'similar to contains 5066 10035 15002 1. 02 7. 8E-02 AI418520. 1 EST HUMAN MER10. t3 MER10 repetitive element ; 3506 8514 2. 61 7. 7E-02 AJ238093. 1 NT Homo sapiens partial AF-4 gene, exons 2 to 7 and Alu repeat elements 3303 8314 13340 2. 57 7. 6E-02 BE514432. 1 EST HUMAN 601316426F1 NIH MGC 8 Homo sapians cDNA clone IMAGE : 3634903 5' 3324 8334 13354 0. 82 7. 6E-02 AA296447. 1 EST HUMAN EST112214 Cerebellum II Homo sapiens cDNA 5'end similar to similar to protocadherin 43 776 5798 10826 1. 86 7. 5E-02 5902093 NT Homo sapiens solute carrier family 6 (neurotransmitter transporter, glycine), member 9 (SLC6A9), mRNA- 776 5798 10827 1. 86 7. 5E-02 5902093 NT Homo sapiens solute carrier family 6 (neurotransmitter transporter, glycine), member 9 (SLC6A9), mRNA. 1877 6866 11954 1. 15 7. 5E-02 AL163278. 2 NT Homo sapiens chromosome 21 segment HS21 C078 4377 9368 14348 0. 92 7. 5E-02 AB015961. 1 NT Homo sapiens IL-18 gene for interleukin-18, intron 1 and exon 2- 474 5510 10523 1. 1 7. 4E-02 AW 838547. 1 EST HUMAN RC5-LT0054-260100-011-H09 LT0054 Homo sapiens cDNA 3514 8522 13533 0. 77 7. 4E-02 AI807885. 1 EST HUMAN wF43h01. x1 Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE : 2358385 3'. 4566 9554 14541 1. 18 7. 4E-02 L78810. 1 NT Homo sapiens ADP/ATP carrier protein (ANT-2) gene, complete cds 4650 9635 14628 2. 97 7. 4E-02 6978442 NT Rattus norvegicus Activin recepta like kinase 1 (Acvrl1), mRNA 4784 9768 14752 1. 75 7. 4E-02 6678492 NT Mus musculus ubiquinfin c-terminai hydrolase related polypeptide (Uchrp), mRNA 466 5503 10514 1. 23 7. 3E-02 BE964961. 2 EST HUMAN 601658738R1 NIH MGC 69 Homo sapiens cDNA ctone IMAGE : 3886209 3' 466 5503 10515 1. 23 7. 3E-02 BE964961. 2 EST HUMAN 601658738R1 NIH_MGC 69 Homo sapiens cDNA clone IMAGE : 3886209 3' 676 5701 10709 3. 86 7. 3E-02 AE001789. 1 NT Thermotoga marifima section 101 of 136 of the complete genome 1450 7748 11508 2. 33 7. 3E-02 AW 900281. 1 EST HUMAN CMO-NN1004-130300-284-g08 NN1004 Homo sapiens cDNA- F : Page 38 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: NO: Source Value 1808 7756 11.5 7.3E-02 AL163302.2 NT Homo sapiens chromosome 21 segment HS21C102 4838 9822 1. 14 7. 3E-02 U12283. 1 NT Mus musculus transcription factor USF2 (USF2) gene, exons 8-10 and complete cds Methanobacterium thermoautotrophicum from bases 1029155 to 1039934 (section 88 of 148) of the complete 120 5190 10202 1. 2 7. 2E-02 AE000882. 1 NT genome Methanobacterium thermoautotrophicum from bases 1029155 to 1039934 (section 88 of 148) of the complete 120 5190 10203 1. 2 7. 2E-02 AE000882. 1 NT genome 1446 6443 11501 1. 67 7. 2E-02 AL163301. 2 NT Homo sapiens chromosome 21 segment HS21 C101 1446 6443 11502 1. 67 7. 2E-02 AL163301. 2 NT Homo sapiens chromosome 21 segment HS21 C101 Human immunodeficiency virus type 1 isolate 26 reverse transcriptase (pol) gene, internal fragment, partial 2478 7447 1. 86 7. 2E-02 U14794. 1 NT cds 3791 8794 13799 1. 62 7. 2E-02 AW298322. 1 EST_HUMAN UI-H-BW0-ajl-a-05-0-UI.s1 NCI_CGAP_Sub6 Homo sapiens cDNA clone IMAGE : 2732049 3' 4220 9213 14192 5. 17 7. 2E-02 BF572307. 1 EST HUMAN 602077757F1 NIH_MGC_62 Homo sapiens cDNA clone IMAGE : 4251950 5' 1863 6852 11940 1. 58 7. 1E-02 L02290. 1 NT Human immunodeficiency virus type 1 (D9) proviral structural capsid protein (gag) gene, partial cds 2225 7202 1. 06 7. 1 E-02 AE004890. 1 NT Pseudomonas aeruginosa PA01, section 451 of 529 of the complete genome 2230 7207 12322 5. 78 7. 1E-02 BF208902. 1 EST_HUMAN 601872281F1 NIH_MGC_53 Homo sapiens cDNA clone IMAGE : 4092981 5' ba10b05. y1 NIH MGC 7 Homo sapiens cDNA clone IMAGE : 2823921 5'similar to gb : X52851_rna1 PEPTIDYL-PROLYL CIS-TRANS ISOMERASE A (HUMAN) ; gb : X52803 Mouse mRNA for cyclophilin 4997 9969 14946 1. 09 7. 1E-02 BE208576. 1 EST HUMAN (MOUSE) ; 524 5559 10562 1. 16 7. 0E-02 Q07092 SWISSPROT COLLAGEN ALPHA 1 (XVI) CHAIN PRECURSOR 1470 64670. 92 7. OE-02 X96677. 1 NT M. artiellia Mtcut-1 gene 1724 6719 11797 1. 17 7. OE-02 AA056343. 1 EST_HUMAN zi66f04.s1 Stratagene colon (#937204) Homo sapiens cDNA clone IMAGE : 509599 3' 2960 7979 12993 2. 25 7. OE-02 AW138152. 1 EST_HUMAN HI-H-BI1-acy-c-07-0-UI.s1 NCI_CGAP_Sub3 Homo sapiens cDNA clone IMAGE : 2716020 3' ai65a12. s1 Soares testis NHT Homo sapiens cDNA clone 1375678 3'similar to gb : K03002 60S 3802 8805 13810 0. 82 7. 0E-02 AA815438. 1 EST HUMAN RIBOSOMAL PROTEIN L32 (HUMAN) ; _ 3931 8931 13922 1. 07 7. 0E-02 BE070264. 1 EST HUMAN QV4-BT0407-280100-090-e10 BT0407 Homo sapiens cDNA 4017 9013 0.96 7.0E-02 AW792962. 1 ESTHUMAN CMO-UM0001-060300-270-e12UM0001 Homo sapiens cDNA 4093 9087 14076 1. 2 7. 0E-02 AF077821. 1 NT Canis familiaris inducible nitric oxide synthase mRNA, complete cds 4773 9757 14745 7. 8 7. OE-02 BF381987. 1 EST_HUMAN 601816291F1 NIH_MGC_56 Homo sapiens cDNA clone IMAGE : 4050071 5' 510 5545 10548 17. 58 6. 9E-02 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21 C010 510 5545 10549 17. 58 6. 9E-02 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 1313 6310 1. 29 6. 9E-02 4507968 NT Homo sapiens regulator of Gz-selective protein signaling (ZGAP1) mRNA, and translated products 3703 8707 13709 1. 03 6. 9E-02 Q06364 SWISSPROT 26S PROTEASOME REGULATORY SUBUNIT S3 (NUCLEAR ANTIGEN 21D7) Page 39 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databas NO NO : Signal BLAST E No. Source NO: NO: Source Value 3703 8707 13710 1.03 6.9E-02 Q06364 SWISSPROT 26S PROTEASOME REGULATORY SUBUNIT S3 (NUCLEAR ANTIGEN 21D7) ae30f02. r1 Gessler Wilms tumor Homo sapiens cDNA clone IMAGE : 897339 5'similar to gb : M22382 1843 6833 11920 1. 06 6. 8E-02 AA496759. 1 EST HUMAN MITOCHONDRIAL MATRIX PROTEIN P1 PRECURSOR (HUMAN) ; ae30f02. r1 Gessler Wilms tumor Homo sapiens cDNA clone IMAGE : 897339 5'similar to gb : M22382 1843 6833 11921 1. 06 6. 8E-02 AA496759. 1 EST HUMAN MITOCHONDRIAL MATRIX PROTEIN P1 PRECURSOR (HUMAN) ; 1865 6854 11942 3. 07 6. 8E-02 AF156673. 1 NT Homo sapiens putative hepatic transcription factor (WBSCR14) gene, complete cds 3027 8044 13053 1. 01 6. 8E-02 AA781996. 1 EST_HUMAN ai75a06.s1 Soares_testis_NHT Homo sapiens cDNA clone 1376626 3' 3027 8044 13054 1. 01 6. 8E-02 AA781996. 1 EST_HUMAN al75a06. s1 Soares testis NHT Homo sapiens cDNA clone 1376626 3' 3027 8044 13055 1. 01 6. 8E-02 AA781996. 1 EST_HUMAN al75a06.s1 Soares_testis_NHT Homo sapiens cDNA clone 1376626 3' 1497 6495 1. 66 6. 7E-02 AF115536. 1 NT Oncorhynchus mykiss TAP1 protein (OnmyTAP1) mRNA, OnmyTAP1*01 allele, complete cds 1854 6843 11931 1. 32 6. 7E-02 AI220285. 1 EST_HUMAN qg79e04.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 1841406 3' 3635 8641 13646 4. 56 6. 7E-02 P17278 SWISSPROT HOMEOBOX PROTEIN HOX-D4 (CHOX-A) at12eO9. x1 Barstead aorta HPLRB6 Homo sapiens cDNA clone IMAGE : 2354920 3'similar to 1330 6328 11376 1. 14 6. 6E-02 AI735509. 1 EST HUMAN SW : LIN1 NYCCO P08548 LINE-1 REVERSE TRANSCRIPTASE HOMOLOG. ; 1352 6349 11399 0. 98 6. 6E-02 AF245116. 1 NT Drosophila melanogaster cactin mRNA, complete cds 2117 7097 12210 1. 74 6. 6E-02 AJ289241. 1 NT Mus musculus Capn12 gene for calpain 12, exons 1-21, three alternative transcipts 3382 8390 13413 9. 7 6. 6E-02 R64306. 1 EST HUMAN yi8b10. s1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 139579 3' 3398 8406 13432 2. 63 6. 6E-02 7108357 NT Homo sapiens mesothelin (MSLN), transcript variant 1, mRNA 3398 8406 13433 2. 63 6. 6E-02 7108357 NT Homo sapiens mesothelin (MSLN), transcript variant 1, mRNA 3965 8963 13953 1. 53 6. 6E-02 AF260225. 1 NT Homo sapiens TESTIN 2 and TESTIN 3 genes, complete cds, alternatively spliced 4819 9803 14783 9. 95 6E-02 Q61703 Q61703 SWISSPROT INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H2 PRECURSOR (ITI HEAVY CHAIN H2) 4819 9803 14784 9. 95 6. 6E-02 Q61703 SWISSPROT INTER-ALPHA-TRYPSIN INHIBITOR HEAVY CHAIN H2 PRECURSOR (ITI HEAVY CHAIN H2) 577 5609 10608 2 6. 5E-02 BF027639. 1 EST HUMAN 601671046F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3954178 5' 972 5988 11022 3. 15 6. 5E-02 7706068 NT Homo sapiens E2F-like protein (LOC51270), mRNA 1368 6365 11414 2.48 6. 5E-02 U47624. 1 NT Xenopus laevis alpha (E)-catenin mRNA, complete cds 1697 6692 11768 1. 65 6. 5E-02 AE000764. 1 NT Aquifex aeolicus section 96 of 109 of the complete genome 570 5603 10601 1. 52 6. 4E-02 X94549. 1 NT A. carterae precursor of peridinin-chlorophyila-protein (PCP) gene 1695 6690 11765 0. 96 6. 4E-02 AE001777. 1 NT Thermotoga maritima section 89 of 136 of the complete genome 1695 6690 11766 0. 96 6. 4E-02 AE001777. 1 NT Thermotoga maritima section 89 of 136 of the complete genome 2946 7965 12984 1. 88 6. 4E-02 6996923 NT Mus musculus histone deacetylase 5 (Hdac5), mRNA 4928 9905 14883 1. 21 6. 4E-02 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21 C047 Mus musculus major histocompatibility locus class tit regions Hsc70t gene, partial cds ; smRNP, G7A, NG23, 1716 6711 11788 2. 39 6. 3E-02 AF109905. 1 NT MutS homolog, CLCP, NG24, NG25, and NG26 genes, complete cds ; and unknown genes Page 40 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORFSEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO : Signal BLAST E No. Source Top Hit Descriptor Value 3523 8530 2. 55 6. 3E-02 P37092 SWISSPROT HEAT SHOCK PROTEIN 70 HOMOLOG 4129 9124 14108 3.54 6. 2E-02 AL161572. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 68 Rattus norvegicus differentation-associated Na-dependent inorganic phosphate cotransporter (DNPI) mRNA, 4212 9205 1 6. 2E-02 AF271235. 1 NT complete cds 4450 9440 5.96 6. 2E-02 Q62191 SWISSPROT 52 KD RO PROTEIN (SJOGREN SYNDROME TYPE A ANTIGEN (SS-A)) (RO (SS-A)) (R052) yg97a12. s1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 41477 3'similar to gb : X57198_cds1 5016 9987 14963 1.58 6. 2E-02 R59526. 1 EST_HUMAN TRANSCRIPTION ELNGATION FACTOR S-II (HUMAN) ; 254 5314 10324 3. 36 6. 1E-02 D16471. 1 NT Human mRNA, Xq terminal portion 3885 8886 2. 17 6. 1E-02 U73325. 1 NT Arabidopsis thaliana K+ inward rectifying channel protein (AtKC1) gene, complete cds 5000 9971 14947 0. 92 6. 1E-02 Y12503. 1 NT S.scrofa mRNA for Man9-mannosidase 5018 9989 1. 37 6. 1E-02 AI220330. 1 EST HUMAN qg90e08. x1 Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE : 1842470 3' 1243 6241 11282 0. 88 6. 0E-02 AE001777. 1 NT Thermotoga maritima section 89 of 136 of the complete genome Mesocestoides coti mitochondrial DNA, NADH dehydrogenase subunit 4, tRNA-Gln, tRNA-Phe, tRNA-Met, 2700 7657 2.19 6. 0E-02 AB031289. 1 NT ATPase subunit 6, and NADH dehydrogenase subunit 2 2866 5173 10183 0. 91 6. OE-02 AA188730. 1 EST HUMAN zp78c04. r1 Stratagene HeLa cell s3 937216 Homo sapiens cDNA clone IMAGE : 626310 5' 2866 5173 10184 0. 91 6. 0E-02 AA188730. 1 ESTHUMAN zp78c04. r1 Stratagene HeLa cell s3 937216 Homo sapiens cDNA clone IMAGE : 626310 5' 3158 8174 13196 1. 62 6.0E-02 AA372376. 1 EST_HUMAN EST84266 Colon adenocarcinoma IV Homo sapiens cDNA 5' end similar to tissue-specific protein 3158 8174 13197 1. 62 6. 0E-02 AA372376. 1 EST HUMAN EST84266 Colon adenocarcinoma IV Homo sapiens cDNA 5'end similar to tissue-specific protein 3549 8556 2. 87 6. OE-02 BE964443. 2 EST HUMAN 601658150R1 NIH_MGC 68 Homo sapiens cDNA clone IMAGE;3876060 3' 229 5292 10300 6. 97 5. 9E-02 AW934719. 1 EST_HUMAN RC1-DT0001-290100-012-e10 DT0001 Homo sapiens cDNA 2914 7933 12952 2. 79 5. gE-02 AF190269. 1 NT Mus musculus p53 tumor suppressor gene, exon 10 and 11, partial cds ; alternatively spliced 920 5936 3. 97 5. 8E-02 D90110. 1 NT Thiobacillus ferrooxidans merC, merA genes and URF-1 1620 6617 11683 2. 52 5. 8E-02 Q61768 SWISSPROT KINESIN HEAVY CHAIN (UBIQUITOUS KINESIN HEAVY CHAIN) (UKHC) 2789 7810 0. 94 5. 8E-02 AJ223621. 1 NT Populus trichocarpa CCoAOMT1 gene, exon 1 to exon 5 3579 8586 13591 1. 8 5.8E-02 AE001775. 1 NT Thermotoga maritima section 87 of 136 of the complete genome 4229 9223 14204 5. 55 5. 8E-02 AW051927. 1 EST_HUMAN wx24c02.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2544578 3' 4229 9223 14205 5. 55 5. 8E-02 AW051927. 1 ESTHUMAN wx24c02. x1 NCiCGAPKid11 Homo sapiens cDNA clone IMAGE : 2544578 3' qh56f01. x1 SoaresfetaiJiverspieen1NFLSS1 Homo sapiens cDNA clone IMAGE : 1848697 3'similar to 4416 9406 14392 4. 67 5. 8E-02 AI247505. 1 EST HUMAN gb : M13142 COAGULATION FACTOR Xi PRECURSOR (HUMAN) ; qh56f01.x1 Soares_fetal_liver_spieen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 1848697 3'similar to 4416 9406 14393 4. 67 5. 8E-02 AI247505. 1 EST HUMAN gb : M13142 COAGULATION FACTOR XI PRECURSOR (HUMAN) ; 4441 9431 2.28 5. 8E-02 AF096264. 1 NT Gallus gallus tyrosine kinase JAK1 (JAK1) mRNA, complete cds 4992 996414942 4. 11 5. 8E-02 7657006 NT Homo sapiens dual adaptor of phosphotyrosine and 3-phosphoinositides (DAPP1), mRNA Page 41 of 209 Table 4 Sinale Exon Probes Exnressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor NO: NO: ID NO: Signal BLASTE E No. Source Valut . ou63b05.s1 NCI_CGAP_Br2 Homo sapiens cDNA clone IMAGE : 1632465 3' similar to WP : C37A2. 2 2983 8001 13013 1. 08 5. 7E-02 AI081644. 1 EST_HUMAN CE08611 ; 2998 8016 13029 1. 11 5. 7E-02 AF119117. 1 NT Homo sapiens dopamine transporter (SLC6A3) gene, complete cds Chironomus thummi thummi globin VIIA. 1 (ctt-7A. 1), globin 9. 1 (ctt-9. 1), globin II-beta (ctt-2beta), non- 3625 8632 1. 02 5. 7E-02 AF001292 1 NT functionai globin Xlil (ctt-13RT), globin XII (ctt-12) and globin Xl (ctt-11) genes, complete cds 3712 8716 13718 2. 21 5. 7E-02 AW966791. 1 EST HUMAN EST378865 MAGE resequences, MAGI Homo sapiens cDNA 4546 9535 1. 05 5. 7E-02 M95099. 1 NT Boys taurus lysozyme gene (cow 3), complete cds 1495 6493 11548 1. 7 5. 6E-02 AF094455. 1 NT Hydrocotyie rotundifolia ribosomal protein L16 (rpl16) gene, intron, chloroplastgenefor chloroplast product 2226 7203 1. 01 5. 6E-02 BE904308. 1 EST HUMAN 601494578F2 NIH MGC 70 Homo sapiens cDNA clone IMAGE : 3896610 5' 4506 9496 14474 1. 56 5. 6E-02 AB013100. 1 NT Lycopersicon esculentum LE-ACS6 mRNA for 1-aminocyclopropane-1-carboxylate synthase, complete cds 4557 9545 14530 0. 84 5. 6E-02 AA290599. 1 EST_HUMAN zs45c01.s1 NCI_CGAP_GCG1 Homo sapiens cDNA clone IMAGE : 700416 3' 2583 7546 12660 4. 77 5. 5E-02 X97869. 1 NT H. sapiens gene encoding La autoantigen 3143 8159 13181 3. 85 5. 5E-02 6755501 NT Mus musculus SH3 domain protein 1B (Sh3d1B), mRNA 4095 9089 14077 t. 04 5. 5E-02 L41561. 1 NT Gallid herpesvirus mRNA fragment 1270 6268 0. 75 5. 4E-02 AF157623. 1 NT Homo sapiens HTRA serine protease (PRSS11) gene, complete cds 2953 7972 0. 93 5. 4E-02 AJ277468. 1 NT Oryza sativa rbbi3-1 gene for putative Bowman Birk trypsin inhibitor 3337 10056 6. 65 5. 4E-02 BE073468. 1 EST_HUMAN RC5-BT0559140200-012-C03 BT0559 Homo sapiens cDNA 1036 6046 11075 1. 48 5. 3E-02 AW391248. 1 EST HUMAN QVO-ST0213-021299-062-a09 ST0213 Homo sapiens cDNA 1036 6046 11076 1.48 5. 3E-02 AW391248. 1 EST_HUMAN. QV0-ST0213-021299-062-a09 ST0213 Homo sapiens cDNA ye37f12. r1 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE:119951 5' similar to gb : K01506 1476 6473 11531 7. 24 5. 3E-02 T94759. 1 EST_HUMAN HLA CLASS II HISTOCOMPATIBILITY ANTIGEN, DP (1) ALPHA CHAIN (HUMAN) ; 2424 7395 12516 1.9 5.E-02 AJ276408. 1 NT Pseuldomonas putida ttgS gene 2872 7891 12913 0. 82 5. 3E-02 M58417. 1 NT Drosophila melanogaster laminin B2 gene, complete cds 2872 7891 12914 0. 82 5. 3E-02 M58417. 1 NT Drosophila melanogaster laminin B2 gene, complete cds 3079 8095 13109 4. 71 5. 3E-02 AJ276408. 1 NT Pseudomonas putida ttgS gene 4916 9894 14868 9. 18 5. 3E-02 M80463. 1 NT Mus musculus caudal type homeobox-1 (Cdx-1) gene, complete cds 2221 7198 56. 99 5. 2E-02 5031908 NT Homo sapiens meprin A, alpha (PABA peptide hydroalse) (MEP1A) mRNA 3040 8057 13065 2. 21 5. 2E-02 AJ277661. 1 NT Homo sapiens partial LM01 gene for LIM domain only 1 protein, exon 1 3040 8057 13066 2. 21 5. 2E-02 AJ277661. 1 NT Homo sapiens partial LM01 gene for IM domain only 1 protein, exon 1 4156 9151 14133 3. 13 5. 2E-02 U07132. 1 NT Human steroid hormone receptor Ner-I mRNA, complete cds 2303 7278 0. 96 5. 1E-0 AL134071. 1 EST HUMAN DKFZp547D073 r1 547 (synonym : hfbr1) Homo sapiens cDNA clone DKFZp547D073 5' 4845 9827 0.91 5.1E-02 AB031740.1 NT Homo sapiens PBII gene for salivary proline-rich protein P-B, complete cds Page 42 of 209 Table 4 Single Exon Probes Expressed in NBL1Q0 Celis Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Eression (Top) Hit Top Hit Acession pabase Top NO : NO : NO : Signal BLAST E N Sourca, Value 479 5516 10526 1. 87 5. OE-02 AF098004. 1 NT Mus musculus fatty acid amide hydrdase gene, exon 10 1185 6186 11223 12. 26 5. OE-02 Z99104. 1 NT Bacillus subtilis complete genome (section 1 of 21) : from 1 to 213080 SALIVARYACIDIC PROLINE-RICH PHOSPHOPROTEIN 1I2 PRECURSOR (PRP-1IPRP-3) (PRP-2IPRP 1944 6930 12029 2. 86 5. OE-02 P02810 SW ISSPROT 4) (PIF-F/PIF-S) (PROTEIN AIPROTEIN C) [CONTAINS : PEPTIDE P-C] 2746 5987 11021 1. 3 5. OE-02 U72742. 1 NT Oryctolagus cuniculus UDP-glucuronosylUansferase (UGT2B13) mRNA, complete cds 3263 8276 1. 24 5. OE-02 7305610 NT Mus musculus Unc-51 like kinase 2 (C. elegans) (UIk2), mRNA 3515 8523 1. 04 S. OE-02 U32782. 1 NT Haemophilus influenzae Rd sec6on 97 of 163 of the complete genome 3600 8607 13615 7. 06 5. OE-02 U12769. 2 NT Antheraea pernyi period clock protein homdog mRNA, complete cds 223 5285 30. 11 4. 9E-02 M14230. 1 NT Chicken 28-kDa vitamin D-dependent calcium-binding protein (CaBP-28) mRNA, complete cds 368 5417 10431 2. 47 4. 9E-02 AF275948. 1 NT Homo sapiens ABCA1 (ABCA1) gene, compiete cds 368 5417 10432 2. 47 4. 9E-02 AF275948. 1 NT Homo sapiens ABCA1 (ABCA1) gene, complete cds 2803 7823 12838 0. 89 4. 9E-02 U32636. 1 NT Zea mays phytoene synthase (Y1) gene, complete cds 3217 8232 13253 1. 52 4. 9E-02 P54258 SW ISSPROT ATROPHIN-1 (DENTATORUBRAL-PALLIDOLUYSIAN ATROPHY PROTEIN) zq48a12. s1 Stratagene hNT neuron (#1937233) Hano sapiens cDNA clone IMAGE : 632926 3'similar to 3490 8498 2. 64 4. 9E-02 AA188940. 1 EST I-IUMAN contains Alu repetitive element ; contains element MSR1 repeti6ve element ; 3511 8519 13530 0. 71 4. 9E-02 AA400914. 1 EST HUMAN zt78a03. s1 Soares testis_NHT Homo sapiens cDNA clone IMAGE : 728428 3' 3511 8519 13531 0. 71 4. 9E-02 AA400914. 1 EST HUMAN zt78a03. s1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 728428 3' 4692 9677 14660 2. 02 4. 9E-02 AW 167821. 1 EST HUMAN xg56g10. x1 NCI_CGAP Ut4 Homo sapiens cDNA clone IMAGE : 2632386 3' 4692 9677 14661 2. 02 4. 9E-02 AW 167821. 1 EST HUMAN xg56g10. x1 NCI_CGAP Ut4 Homo sapiens cDNA clone IMAGE : 2632386 3' 328 5381 10390 1. 13 4. 8E-02 D16471. 1 NT Human mRNA, Xq terminal portion 329 5381 10390 2. 65 4. 8E-02 D16471. 1 NT Human mRNA, Xq terminal portion 485 5522 10531 8. 84 4. 8E-02 AF003100. 1 NT Arabidopsis thaliana AP2 domain containing protein RAP2. 7 mRNA, partial cds zc49b02. s1 Soares senescent_fibroblasts_NbHSF Homo sapiens cDNA clone IMAGE : 325611 3'similar to. 2211 7188 12309 1. 27 4. 8E-02 W 51983. 1 EST HUMAN gb : M30938 LUPUS KU AUTOANTIGEN PROTEIN P86 (HUMAN) ; _ 3136 8152 13175 2. 05 4. 8E-02 X17144. 1 NT Tefrahymena rostrata histone H311 and histone H411 intergenic DNA 268 5327 10338 1. 22 4. 6E-02 BE153583. 1 EST HUMAN PMO-HT0339-251199-003-g05 HT0339 Homo sapiens cDNA 730 5753 10774 2. 86 4. 6E-02 AE000445. 1 NT Escherichia cdi K-12 MG1655 sec6on 335 of 400 of the complete genome am50d02. s1 Johnston frontal cortex Homo sapiens cDNA cione IMAGE : 1538979 3'similar to TR : P90533 1273 6271 0. 71 4. 6E-02 A1014255. 1 EST_HUMAN P90533 LIMA ; contains element LTR1 repetitive element ; 1342 6339 11390 2. 41 4. 6E-02 AV727059. 1 EST HUMAN AV727059 HTC Homo sapiens cDNA clone HTCBW C01 5' m24f03. x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2694653 3'similar to SW : GRF1 HUMAN- : 2418 7389 12509 2. 09 4. 6E-02 AW236023. 1 EST FiUMAN Q12849 G-RICH SEQUENCE FACTOR-1 ; 2736 5327 10338 2. 36 4. 6E-02 BE153583. 1 EST I-IUMAN PMO-HT0339-251199-003-g05 HT0339 Homo sapiens cDNA 3416 7958 12976 0. 76 4. 6E-02 BE153583. 1 EST HUMAN PMO-HT0339-251199-003-g05 HT0339 Homo sapiens cDNA' Y 'C C Page 43 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Eression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Database Top Hit Descriptor Source NO : NO : Value 4004 9000 0. 86 4. 6E-02 AF220365. 1 NT Mus musculus nucleolar RNA helicase IIIGu (ddx21) gene, complete cds 444 5481 10499 1. 67 4. 5E-02 P22448 SW ISSPROT RETiNOIC ACID RECEPTOR BETA (RAR-BETA) 1199 6200 11236 0. 81 4. 5E-02 AF005730. 1 NT Marburg virus strain MIS. AfricalJohannesburgl197510zolin VP35 gene, complete cds 1199 6200 11237 0. 81 4. 5E-02 AF005730. 1 NT Marburg virus strain MIS. AfricalJohannesburg/197510zolin VP35 gene, complete cds 1768 6760 11847 4. 15 4. 5E-02 P32182 SW ISSPROT HEPATOCYTE NUCLEAR FACTOR 3-BETA (HNF-3B) 2051 7033 12145 2. 17 4. 5E-02 AE003964. 1 NT Xylella fastidiosa, section 110 of 229 of the complete genome 3640 8646 13653 3. 82 4. 5E-02 AL163278. 2 NT Homo sapiens chromosome 21 segment HS21 C078 9 52g2 3. 85 4. 4E-02 BE972733. 1 EST_HUMAN 601652154F1 NIH_MGC 82 Homo sapiens cDNA clone IMAGE : 3935388 5' 2039 7022 3. 33 4. 4E-02 P31568 SW ISSPROT HYPOTHETICAL PROTEIN (ORF 2280) 2419 7390 12510 1. 11 4. 4E-02 AW 875475. 1 EST HUMAN QV2-PT0012-010300-070-g02 PT0012 Homo sapiens cDNA 3555 8562 13568 1. 81 4. 4E-02 AF159160. 1 NT Mycococcus xanthus serineJthreonine kinase Pkn10 (pknl0) gene, complete cds Homo sapiens S164 gene, partial cds ; PS1 and hypothetical protein genes, complete cds ; and S171 gene, 4493 9483 14461 0. 99 4. 4E-02 AF109907. 1 NT partial cds Homo sapiens S164 gene, partial cds ; PS1 and hypothetical protein genes, complete cds ; and S171 gene, 4493 9483 14462 0. 99 4. 4E-02 AF109907. 1 NT partial cds 772 5794 10822 6. 82 4. 3E-02 AF003249. 1 NT Morone saxa6lis myosin heavy chain FM3A (FM3A) mRNA, complete cds 3345 8354 13372 8. 44 4. 3E-02 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 3575 8582 1. 23 4. 3E-02 AF060568. 1 NT Homo sapiens promyelocytic leukemia zinc finger protein (PLZF) gene, complete cds g12 5833 10866 1. 73 4. 2E-02 AU123327. 1 EST HUMAN AU123327 NT2RM2 Homo sapiens cDNA clone NT2RM2000020 5' 855 5874 1. 9 4. 2E-02 AU123327. 1 EST HUMAN AU123327 NT2RM2 Homo sapiens cDNA clone NT2RM2000020 5' wC34g01. x1 NCI CGAP Pit1 Homo sapiens cDNA clone IMAGE : 2545584 3'similar to TR : Q63291 Q63291 885 5903 10944 1. 49 4. 2E-02 AW 003645. 1 EST HUMAN L1 RETROPOSON, ORF2 MRNA ; contains L1. t3 L1 L1 repetitive element ;- 1682 6678 2. 39 4. 2E-02 AL445066. 1 NT Thermoplasma acidophilum complete genome ; segment 415. 3580 8587 13592 1. 51 4. 2E-02 P23091 SW ISSPROT TRANSFORMING PROTEIN MAF 4600 9588 14575 3. 38 4. 2E-02 BF342995. 1 EST HUMAN 602017105F1 NCI_CGAP Brn64 Homo sapiens cDNA clone IMAGE : 4152672 5' 2605 7567 12682 1. 08 4. 1E-02 AE002330. 2 NT Chlamydia muridarum, section 60 of 85 of the complete genome 4347 9338 8. 04 4. 1E-02 AW893484. 1 EST HUMAN QV1-NN0012-180400-164-f06 NN0012 Homo sapiens cDNA 4954 9939 0. 67 4. 1E-02 X85880. 1 NT L. monocytogenes type 3 partial iap gene (strain 443) 1603 6599 11660 0. 9 4. OE-02 AI675392. 1 EST HUMAN wb98h01. x1 NCI_CGAP Pr28 Homo sapiens cDNA clone IMAGE : 2313745 3' 3170 8186 13207 3. 01 4. OE-02 AB040904. 1 NT Homo sapiens mRNA for KIAA1471 protein, partial cds 1103 6110 11141 3. 41 3. 9E-02 BF516149. 1 EST HUMAN UI-H-BW 1-anx h-08-0-UI. s1 NCI_CGAP Sub7 Homo sapiens cDNA clone IMAGE : 3084134 3' 1326 6324 11371 1. 9 3. 9E-02 P41047 SW ISSPROT FAS ANTIGEN LIGAND 1921 6907 12001 1. 85 3. 9E-02 AJ403386. 1 NT M. musculus DNA for desmin-binding fragment DesD7 f Page 44 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Simllar Probe Exon Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor NO : NO : ID NO : Signal BLAST E No. Source Value Homo sapiens succinate dehydrogenase complex, subunit C, integral membrane protein, 15kD (SDHC) 2632 7592 1.51 3.9E-02 4506862 NT mRNA 977 5992 11025 5. 31 3. 7E-02 P19137 SWISSPROT LAMININ ALPHA-1 CHAIN PRECURSOR (LAMININ A CHAIN) 2175 7154 12274 5. 07 3. 7E-02 AI984806. 1 EST_HUMAN wr85e08.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2494502 3' 2502 7470 12586 1. 04 3. 7E-02 AB018261. 1 NT Homo sapiens mRNA for KIAA0718 protein, partial cds 2976 7994 13008 1. 04 3. 7E-02 P79944 SW lSSPROT EOMESODERMlN 2978 7996 13009 3. 76 3. 7E-02 BF312963. 1 EST_HUMAN 601896233F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE:4125584 5' Mus musculus potassium large conductance pH-sensitive channel, subfamily M, alpha member 3 (Kcnma3), 3372 8380 1. 16 3. 7E-02 6680541 NT mRNA 3115 8131 13150 43. 98 3. 6E-02 AP000003. 1 NT Pyrococcus horikoshii OT3 genomic DNA, 544001-777000 nt. position (3/7) 3570 8577 13583 0. 8 3. 6E-02 X73221. 1 NT H. vulgare Ss1 gene for sucrose synthase Homo sapiens genomic region containing hypervariable minisatellites chromosome 10 [10q26. 3] of Homo 3578 8585 13590 0. 73 3. 6E-02 AL096806. 1 NT sapiens 884 5902 10943 1. 6 3. 5E-02 U09506. 1 NT Drosophila melanogaster tiggrin mRNA, complete cds 993 6006 11037 1. 11 3 5E-02 AF253417. 1 NT Homo sapiens microsomal epoxide hydrdase (EPHX1) gene, complete cds 1531 6529 11588 1. 01 3. 5E-02 BF678085. 1 EST_HUMAN 602085136F1 NIH_MGC_83 Homo sapiens cDNA clone IMAGE : 4249377 5' 1531 6529 11589 1. 01 3. 5E-02 BF678085. 1 EST_HUMAN 602085136F1 NIH_MGC_83 Homo sapiens cDNA clone IMAGE : 4249377 5' 4092 9086 14075 3. 28 3. 5E-02 AE001773. 1 NT Thermotoga maritima section 85 of 136 of the complete genome 4189 9182 14165 1. 13 3. 5E-02 P53780 SWISSPROT CYSTATHIONINE BETA-LYASE PRECURSOR (CBL) (BETA-CYSTATHIONASE) (CYSTEINE LYASE) 573 5606 10604 1. 18 3. 4E-02 AK024424. 1 NT HomosapiensmRs _, _ 573 5606 10605 1.18 3. 4E-02 AK024424. 1 NT Homo sapiens mRNA for FLJ00013 protein, partial cds 574 5606 10604 4. 61 3. 4E-02 AK024424. 1 NT Homo sapiens mRNA for FLJ00013 protein, partial cds 574 5606 10605 4. 61 3. 4E-02 AK024424. 1 NT Homo sapiens mRNA for FLJ00013 protein, partial cds xv26d07. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2814253 3'similar to 1034 6044 11073 3. 13 3. 4E-02 AW274020. 1 EST HUMAN SW : C211 HUMAN P53801 PUTATIVE SURFACE GLYCOPROTEIN C210RF1 PRECURSOR ; 1187 6188 9. 31 3. 4E-02 11345459 NT Homo sapiens hypothetical protein FLJ13220 (FLJ13220), mRNA yc20e06. r1 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE : 81250 5'similar to contains ; 2328 7302 12422 1. 86 3. 4E-02 T57160. 1 EST HUMAN MER29 repetitive element 3346 8355 13373 1. 39 3. 4E-02 AL163208. 2 NT Homo sapiens chromosome 21 segment HS21C008. 3688 8692 13694 0. 7 3. 4E-02 BE839514. 1 EST_HUMAN RC3-FN0155-060700-011-d10 FN0155 Homo sapiens cDNA 3821 8823 13830 3. 3 3. 4E-02 AW794952. 1 EST HUMAN RC6-UM0015-210200-021-A10 UM0015 Homo sapiens cDNA 4467 9457 14437 3. 07 3. 4E-02 X59799. 1 NT M. musculus S-antigen gene promoter region 4892 9871 2.79 3.4E-02 Q26457 SWISSPROT LA PROTEIN HOMOLOG (LA RIBONUCLEOPROTEIN) (LA AUTOANTIGEN HOMOLOG) Page 45 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descrintor Va) use Value 4906 9885 14856 1. 61 3. 4E-02 AJ012469. 1 NT Caenorhabditis elegans mRNA for DYS-1 protein, partial 371 5420 15. 62 3. 3E-02 AA398735. 1 EST_HUMAN zt75e08.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE : 728198 3' 1149 6153 11185 14. 2 3. 3E-02 AB035867. 1 NT Cricetuius griseus CYP2A17 mRNA for cytochrome P450 2A17, complete cds 1599 6595 11656 1. 34 3. 3E-02 AF110763. 1 NT Homo sapiens skeletal muscle LIM-protein 1 (FHL1) gene, complete cds 2031 7014 1. 55 3. 3E-02 R09112.1 EST_HUMAN yf26c09.r1 Soaresfetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 127888 5' 4053 6595 11656 2. 72 3. 3E-02 AF110763. 1 NT Homo sapiens skeletal muscle LIM-protein 1 (FHL1) gene, complete cds 4343 9334 14318 2. 06 3. 3E-02 6755862 NT Mus musculus tumor rejection antigen gp96 (Tra1), mRNA 132 5198 10214 2. 52 3. 2E-02 AJ002005. 1 NT Oryctolagus cuniculus gene encoding ileal sodium-dependent bile acid transporter 1109 6115 11145 14. 14 3. 2E-02 AF096275. 1 NT Drosophila melanogaster heat shock protein 68 (hsp68) gene, hsp68d allele, complete cds 1109 6115 11146 14. 14 3. 2E-02 AF096275. 1 NT Drosophila melanogaster heat shock protein 68 (hsp68) gene, hsp68d allele, complete cds 2060 7042 8. 6 3. 2E-02 P28955 SW ISSPROT LARGE TEGUMENT PROTEIN 3061 8078 13091 13. 22 3. 2E-02 BE867353. 1 EST HUMAN 601442431 F1 NIH_MGC_65 Homo sapiens cDNA clone IMAGE : 3846727 5' 3632 8638 13644 1. 3 3. 2E-02 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 3854 8856 13861 0. 88 3. 2E-02 Z74103. 1 NT S. cerevisiae chromosome IV reading frame ORF YDL055c 3854 8856 13862 0. 88 3. 2E-02 Z74103. 1 NT S. cerevisiae chromosome IV reading frame ORF YDL055c 4097 9091 17. 68 3. 2E-02 X94768. 1 NT H. sapiens RP3 gene (XLRP gene 3) 4622 9607 14595 3. 39 3. 2E-02 AF114182. 1 NT Saxdfraga nidifica maturase (matK) gene, chloroplast gene encoding chloroplast protein, partial cds Vitreoscilla sp. outer membrane protein homolog gene, complete cds ; Trp repressor binding protein gene, 4681 9666 14648 2. 57 3. 2E-02 AF067083. 1 NT partial cds ; and unknown genes 1241 6239 1. 62 3. 1E-02 4503416 NT Homo sapiens dual specificity phosphatase 4 (DUSP4) mRNA 1286 6285 11328 1. 44 3. 1E-02 P18845 SWISSPROT NEURONAL ACETYLCHOLINE RECEPTOR PROTEIN, ALPHA-3 CHAIN PRECURSOR (GF-ALPHA-3) 1852 6841 11929 0.95 3. 1 E-02 6671564 NT Mus musculus adaptor-related protein complex AP-3, delta subunit (Ap3d), mRNA 4998 9117 12.58 3. 1 E-02 AU119006. 1 EST HUMAN AU119006 HEMBA1 Homo sapiens cDNA clone HEMBA1004842 5' 5072 10041 5. 51 3. 1E-02 5730074 NT Homo sapiens fibrinogen-like 2 (FGL2), mRNA 1584 6581 11. 29 3. 0E-02 AF187125. 1 NT Pityokteines minutus cytochrome oxidase l gene, partial cds ; mitochondrial gene for mitochondrial product 2509 7477 12592 0. 99 3. 0E-02 AA402242. 1 EST HUMAN zt65h03. r1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 727253 5' 3486 8494 13511 0. 95 3. 0E-02 M94176. 1 NT Saccharomyces cerevisiae stem-loop mutation supressor SSL2 gene, complete cds 3569 8576 13582 2. 92 3. 0E-02 AF247644. 1 NT Pseudomonas fluorescens family 11 aminotransferase gene, complete cds 3660 8665 0. 72 3. OE-02 AW820223. 1 EST_HUMAN QV2-ST0296-15020-040-e09 ST0296 Homo sapiens cDNA 4885 9864 14834 7. 12 3. OE-02 AF281074. 1 NT Homo sapiens neuropilin 2 (NRP2) gene, complete cds, alternatively spliced 48859864 14835 7. 12 3. 0E-02 AF281074. 1 NT Homo sapiens neuropilin 2 (NRP2) gene, complete cds, alternatively spliced . Page 46 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value Homo sapiens mitochondriel glutatione reductase and cytocolle glutathione reductase (GRD1) gene, 2368 7769 12459 1. 06 2. 9E-021AF228703. 1 NT completecds, altemativelyspliced 3827 8829 13836 1. 37 2. 9E-02 H72805.1 EST_HUMAN yu07e10.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 233130 5' MULTIDRUG RESISTANCE-ASSOCIATED PROTEIN 5 (ABC TRANSPORTER MOAT-C) (PABC11) 3877 8878 13881 0. 73 2. 9E-02 015440 SW ISSPROT (SMRP) 4858 9839 14812 1. 81 2. 9E-02 X65137. 1 NT S. vulgare pepC gene for PEP carboxylase 4858 9839 14813 1. 81 2. 9E-02 X65137. 1 NT S. vulgare pepC gene for PEP carboxylase 2406 7377 1 2. 8E-02 AA782516. 1 ESTHUMAN ai55c09. s1 SoaresparathyroidtumorNbHPA Homo sapiens cDNA clone 1360912 3' 3286 8297 13323 1. 1 2. 8E-02 AF066063. 1 NT Homo sapiens retinal fascin (FSCN2) gene, exon 2 3286 8297 13324 1. 1 2. 8E-02 AF066063. 1 NT Homo sapiens retina) fascin (FSCN2) gene, exon 2 4185 9178 0. 8 2. 8E-02 8393751 NT Rattus norvegicus microtubule-associated protein tau (Mapt), mRNA Human germline T-cell receptor beta chain Dopamine-beta-hydroxylase-like, TRY1, TRY2, TRY3, TCRBV27S1P, TCRBV22S1A2N1T, TCRBV9S1A1T, TCRBV7S1A1N2T, TCRBV5S1A1T, TCRBV13S3, TCRBV6S7P, TCRBV7S3A2T, TCRBV13S2A1T, TCRBV9S2A2PT, TCRBV7S2A1N4T, 1457 6454 11513 1. 09 2. 7E-02 U66059. 1 NT TCRBV13S9/13S> 3347 8356 13374 1. 84 2. 7E-02 AL161494. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 6 4079 9073 14060 2. 12 2. 7E-02 N47258. 1 EST_HUMAN yy86h12.r1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE : 280487 5' 4079 9073 14061 2. 12 2. 7E-02 N47258. 1 EST_HUMAN yy86h12.r1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE : 280487 5' ye39f04. s1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 120127 3'similar to contains 5040 10011 14980 1. 04 2. 7E-02 T95073. 1 EST HUMAN Alu repetitive element ; 566 5599 10598 0. 98 2. 6E-02 AL163282. 2 NT Homo sapiens chromosome 21 segment HS21 C082 1350 6347"0. 932. 6E-02AW850515. 1 ESTHUMAN) L3-CT0219-280100-062-C09CT0219 Homo sapiens cDNA' 2305 7280 12398 1. 97 2. 6E-02 AA490021. 1 EST_HUMAN abO2bO2. s1 Stratagene fetal retina 937202 Homo sapiens cDNA clone IMAGE : 839595 3' 2307 7282 12400 1. 99 2. 6E-02 6754241 NT Mus musculus histidine rich calcium binding protein (Hrc), mRNA 2307 7282 12401 1.99 2.6E-02 6754241 NT Mus musculus histidine rich calcium binding protein (Hrc), mRNA Mus musculue MHC class III region RD gene, partial ede; Bf, C2, GOA, NG22, GO, HSP70, HSP70, HSC70t 2845 7865 1. 39 2. 6E-02 AF109906. 1 NT and smRNP genes, complete cds ; G7A gene, partial cds ; and unknown genes 4749 9734 14720 3. 57 2. 6E-02 L12032. 1 NT Chicken dorsalin-1 MRNA, complete cds 4759 9743 14728 1. 27 2. 6E-02 AJ403239. 1 NT M. musculus DNA for vimentin-binding fragment VimE7 4759 9743 14729 1. 27 2. 6E-02 AJ403239. 1 NT M. musculus DNA for vimentin-binding fragment VimE7 4897 9876 14844 1.74 2.6E-02 AE002014.1 NT Deinococcus radiodurans R1 section 151 of 229 of the complete chromosome 1 xa52b04. x1 NCI CGAP Sar4 Homo sapiens cDNA clone IMAGE : 2570383 3'similar to SW : Y069 HUMAN 4921 9899 14874 2.61 2.6E-02 AW241154.1 EST_HUMAN Q15041 HYPOTHETICAL PROTEIN KIAA0069 ; 528 5563 10566 1. 4 2. 5E-02 AI793130. 1 EST HUMAN on26f06. y5 NCI CGAP Lu5 Homo sapiens cDNA clone IMAGE : 1557827 5' Page 47 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID ID NO: Signal BLAST E No. Source NO: NO: Source Value 528 5563 10567 1.4 2.5E-02 AI793130.1 EST_HUMAN on26f06.y5 NCI_CGAP_Lu5 Homo sapiens cDNA clone IMAGE: 1557827 5' 800 5821 10851 12. 78 2. 5E-02 BE974314. 1 EST HUMAN 601680305R2 NIH_MGC 83 Homo sapiens cDNA clone IMAGE : 3950665 3' 858 5877 10918 4.21 2.5E-02 BE974314. 1 EST HUMAN 601680305R2 NIH MGC 83 Homo sapiens cDNA clone IMAGE : 3950665 3' 2689 7647 1.83 2.5E-02 U12571.1 NT Rattus norvegicus rabphilin-3A mRNA, complete cds 2885 7904 129243. 252. 5E-02 X99697. 1 NT H. carterae mRNA for fucoxanthin chlorophyil a/c binding protein, Fcp1 2885 7904 12925 3. 25 2.5E-02 X99697. 1 NT H. carterae mRNA for fucoxanthin chlorophyl a/c binding protein, Fcp1 i 3932 10049 13923 0.85 2.5E-02 BE701165.1 EST_HUMAN PM2-NN0128-080700-001-a12 NN0128 Homo sapiens cDNA 3932 10049 13924 0. 85 2. 5E-02 BE701165. 1 ESTHUMAN PM2-NN0128-080700-001-a12 NN0128 Homo sapiens cDNA 4083 9077 14065 5. 51 2. 5E-02 AW592114. 1 EST_HUMAN hf36h08.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2934015 3' 173 5237 10249 0.68 2.4E-02 AI378582.1 EST_HUMAN tc72c07.x1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE : 2070156 3' 1563 6560 11622 1. 86 2. 4E-02 H65884. 1 EST_HUMAN yr75f11.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 211149 5' 1993 7760 12082 1.31 2.4E-02 P01901 SISSPROT H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, K-B ALPHA CHAIN PRECURSOR (H-2K (B)) 1993 7760 12083 1. 31 2. 4E-0 P01901 SWlSSPROT H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, K-B ALPHA CHAIN PRECURSOR (H-2K (B)) 4243 9237 14220 1. 46 2.4E-02 J05110. 1 NT T. thermophila calcium-binding 25 kDa (TCBP 25) protein mRNA, complete cds 4390 9381 14362 1.56 2. 4E-02 P01901 SWISSPROT H-2 CLASS I H1STOCOMPATIBILITY ANTIGEN, K-B ALPHA CHAIN PRECURSOR (H-2K (B)) 4390 9381 143631. 562. 4E-02 P01901 SW ISSPROT H-2 CLASS I HISTOCOMPATIBILITY ANTIGEN, K-B ALPHA CHAIN PRECURSOR (H-2K (B)) 1834 6824 3. 68 2.3E-02 W05340. 1 EST_HUMAN za84g08.r1 Soares_fetal_lung_NbHL19W Homo sapiens cDNA clone IMAGE : 299294 5' 1848 6837 5.18 2.3E-02 U94165.1 NT 4 Homo sapiens mammary tumor-associated protein INT6 (INT6) gene, exon 4 2289 7264 12382 1. 88 2. 3E-02 Z74293. 1 NT S. cerevisiae chromosome IV reading frame ORF YDL245c 3598 8605 13613 5. 37 2. 3E-02 Z20377. 1 EST HUMAN HSAAACADH P, Human foetal Brain Whole tissue Homo sapiens cDNA 4026 9022 14009 0. 76 2. 3E-0 L24799. 1 NT Gallus gallus connexin 45. 6 (Cx45. 6) gene, complete cds 4026 9022 14010 0.76 2.3E-02 L24799.1 NT Gallus gallus connexin 45.6 (Cx45.6) gene, complete cds 4296 9288 14275 1. 14 2. 3E-02 AW899107. 1 EST HUMAN CM4-NN0080-290400-160-b04 NN0080 Homo sapiens cDNA 4327 9319 14301 0. 85 2. 3E-02 BE935225. 1 EST HUMAN CM3-MT0118-010900-318-g07 MT0118 Homo sapiens cDNA 4327 9319 14302 0. 85 2. 3E-02 BE935225. 1 EST HUMAN CM3-MT0118-010900-318-g07 MT0118 Homo sapiens cDNA 4328 10051 14303 0.82 2.3E-02 AW593693. 1 EST_HUMAN xs25d08.x1 NCI_CGAP_Ut2 Homo sapiens cDNA clone IMAGE : 2770671 3' 4328 10051 14304 0. 82 2. 3E-0 AW593693. 1 EST_HUMAN xs25d08.x1 NCI_CGAP_Ut2 Homo sapiens cDNA clone IMAGE : 2770671 3' 4463 9453 14434 2. 76 2.3E-02 BF026487. 1 EST_HUMAN 601672279F1 NIH_MGC_20 Homo sapiens cDNA clone IMAGE : 3955386 5' 4463 9453 14435 2.76 2.3E-02 BF026487. 1 EST_HUMAN 601672279F1 NIH_MGC_20 Homo sapiens cDNA clone IMAGE:3955386 5' 4860 9841 0. 95 2. 3E-02 7662173 NT Homo sapiens KIAA0547 gene product (KIAA0547), mRNA 4977 9953 14931 0. 77 2. 3E-02 AF257110. 1 NT Rattus norvegicus guanine nucleotide binding protein gamma subunit 11 mRNA, complete cds 4977 9953 14932 0.77 2.3E-02 AF257110. 1 NT Rattus norvegicus guanine nucleotide binding protein gamma subunit 11 mRNA, complete cds Columba livia nucloside diphosphate kinase (NDPK) gene, nuclear gene encoding mitochondrial protein, L 728 5751 10772 2.95 2.2E-02 AF018267.1 NT complete cds Page 48 of 209 Table 4 Single Exon Probes Expressed in HBL100 P Pobe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No, Database Top Hit Descriptor NO : NO : Source, Value 1708 6703 1. 21 2. 2E-02 4557448 NT Homo sapiens chromodomain helicase DNA binding protein 2 (CHD2) mRNA 1723 6718 11795 0, 97 2. 2E-02 P07313 SW ISSPROT MYOSIN LIGHT CHAIN KINASE, SKELETAL MUSCLE 1723 6718 11796 0. 97 2. 2E-02 P07313 SW ISSPROT MYOSIN LIGHT CHAIN KINASE, SKELETAL MUSCLE (MLCK) 1965 6950 12053 1. 43 2. 2E-02 Z82001. 1 NT S. pneumoniae pcpA gene and open reading fames 3350 8359 1. 88 2. 2E-02 AA577785. 1. EST HUMAN nn24a04. s1 NCI_CGAP Gas1 Homo sapiens cDNA clone IMAGE : 1084782 3' 3561 8568 3. 57 2. 2E-02 AF083094. 1 NT Infectious bursal disease virus segment B strain IL4 VP1 gene, complete cds 3763 8766 13769 1. 18 2. 2E-02 AW 601317. 1 EST HUMAN PMO-BT0340-170100-004-b03 BT0340 Homo sapiens cDNA 3820 8822 13829 0. 68 2. 2E-02 Z74293. 1 NT S. cerevisiae chromosome IV reading frame ORF YDL245c 416 5453 4. 33 2. 1E-02 AV761502. 1 EST HUMAN AV761502 MDS Homo sapiens cDNA clone MDSADG01 5' 446 5483 7. 77 2. 1E-02 AF029726. 1 NT Dictyostelium discoideum histidine kinase C (dhkC) mRNA, complete cds Bacillus subtilis cotKLM cluster, CotK (cotK), CotL (cotL), and spore coat protein CotM (cotM) genes, 1244 6242 11283 7. 23 2. 1 E-02 U72073. 1 NT complete cds 1744 6739 11816 1. 17 2. 1E-02P02438 SWISSPROT KERATIN, HIGH-SULFURMATRIXPROTEIN, B2A 1744 6739 11817 1. 17 2. 1E-02 P02438 SW ISSPROT KERATIN, HIGH-SULFUR MATRIX PROTEIN, B2A 1744 6739 11818 1. 17 2. 1 E-02 P02438 SW ISSPROT KERATIN, HIGH-SULFUR MATRIX PROTEIN, B2A 2744 5792 10820 3. 7 2. 1E-02 N29266. 1 EST HUMAN yx43h07. r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 264541 5' 3502 8510 13524 0. 94 2. 9E-02 AA461271. 1 EST HUMAN ac63b09. r1 Soares total_fetus Nb2HF8 9w Homo sapiens cDNA clone IMAGE : 796121 5' 4009 9005 13993 0. 67 2. 1 E-02 Z74293. 1 NT S. cerevisiae chromosome IV reading frame ORF YDL245c 4182 9175 14159 0. 8 2. 1E-02 BF343655. 1 EST HUMAN 602015306F1 NCI CGAP Bm64 Homo sapiens cDNA clone IMAGE : 4151161 5' 4322 9314 14297 1. 92 2. 1E-02 U44914. 1 NT Barelia burgdorferi plasmid cp32-2, erpC and erpD genes, complete cds ; and unknown genes 4333 9324 14310 1. 35 2. 1E-02 AI768127. 1 EST HUMAN w981d11. x1 Soares NSF_F8 9W OT PA P S1 Homo sapiens cDNA clone IMAGE : 2371509 3' 4540 9358 0. 68 2. 1E-02 Y19213. 1 NT Homo sapiens putative psihHbA pseudogene fa hair kera6n, exons 2 to 7 4572 9560 14549 5. 38 2. 1 E-02 Y08501. 1 NT A. thaliana mitochondrial genome, part A 4591 9579 14569 0. 76 2. 1E-02 AA665737. 9 EST HUMAN a955g12. s1 Gessler Wilms tumor Homo sapiens cDNA clone IMAGE : 1126918 3' 7g51c08. x1 NCI_CGAP Pr28 Homo sapiens cDNA clone IMAGE : 3309998 3'similar to contains MER1. t3 _ 6pg7 10080 1. 78 2. OE-02 BF002932. 1 EST HUMAN MER1 repetitiveefement ; 18 5098 10081 9. 9 2. OE-02 AW 895565. 1 EST HUMAN QV4-NN0038-270400-187-h05 NN0038 Homo sapiens cDNA 257 5317 10326 2. 81 2. OE-02 6753635 NT Mus musculus DinB homolo9 1 (E. coli) (Dinb1), mRNA 293 5350 10363 1. 85 2. OE-02 AA456538. 1 EST HUMAN aa15b10. r1 Soares NhHMPu S1 Homo sapiens cDNA clone IMAGE : 813307 5' 790 5811 10841 1. 4 2. OE-02 6753635 NT Mus musculus DinB homolog 1 (E. coli) (Dinb1), mRNA Homo sapiens genomic region containing hypervariabla minisatellites chromosome 1 [1 p36. 33] af Homo 1071 6079 11110 1 2. OE-02 AL096805. 1 NT sapiens 1180 6182 11219 1. 31 2. OE-02 8922391 NT Homo sapiens hypothetical protein FLJ10379 (FLJ10379), mRNA 1180 6182 11220 1. 31 2. OE-02 8922391 NT Homo sapiens hypothetical protein FLJ10379 (FLJ10379), mRNA L. Page 49 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Database Top Hit Descriptor NO : NO : Value Souce, 1835 6825 11912 1. 69 2. OE-02 8922453 NT Homo sapiens hypothefical protein FLJ10486 (FLJ10486), mRNA 1835 6825 11913 1. 69 2. OE-02 8922453 NT Homo sapiens hypothetical protein FLJ10486 (FLJ10486), mRNA 2726 7683 1. 8 2. OE-02 AL161532. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 32 7g51c08. x1 NC CGAP Pr28 Homo sapiens cDNA clone IMAGE : 3309998 3'similar to contains MER1. t3 3005 5097 10080 1. 66 2. OE-02 BF002932. 1 EST HUMAN MER1 repetitive element ; Mus musculus sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6B 3079 8087 2. 21 2. OE-02 7305474 NT (Sema6b), mRNA 3156 8172 1. 54 2. OE-02 AF095588. 1 NT Aabidopsis thaliana C2H2 zinc finger protein FZF mRNA, complete cds 3900 8900 13898 1. 61 2. OE-02 M18095. 1 NT P. wlgaris hydroxyprdine-rich glyooprotein (HRGP) mRNA, 3'end 4943 9920 0. 84 2. OE-02 AI271995. 1 EST HUMAN c1I83e03. x1 NCI CGAP ICid3 Homo sapiens cDNA clone IMAGE : 1866076 3' nf19a07. s1 NCI CGAP Pr1 Homo sapiens cDNA clone IMAGE : 914196 similar to contains L1. t1 L1 684 5708 10720 1. 76 1. 9E-02 AA572764. 1 EST HUMAN repefitiveelement ; 1988 6973 12077 2. 16 1. 9E-02 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 1988 6973 12078 2. 16 1. 9E-02 AL163303. 2 NT Homo sapiens chromosane 21 segment HS21 C103 2434 7405 12523 1. 18 1. 9E-02 AL161550. 2 NT Arabidopsis thaliana DNA chromosome 4, conGg fragment No. 50 2835 7855 12874 7. 79 1. 9E-02 AA713856. 1 EST FiUMAN nw04f05. s1 NCI_CGAP SS1 Homo sapiens cDNA clone IMAGE : 1238337 3' 2881 7900 12922 1. 67 1. 9E-02 AV648669. 1 EST HUMAN AV648669 GLC Homo sapiens cDNA clone GLCBLH07 3' 3185 8201 0. 75 1. 9E-02 AB033611. 1 NT Urotrichus talpoides mitochondriat gene for cytochrome b, complete cds 3530 8536 0. 91 1. 9E-02 N52250. 1 EST_HUMAN Y8b02. s1 Soares multiple sclerosis 2NbHMSP Homo sapiens cDNA clone IMAGE : 284331 3' 3622 8629 8. 78 1. 9E-02 BE738088. 1 EST HUMAN 601572682F1 NIH_MGC 57 Homo sapiens cDNA clone IMAGE : 3839564 5' qn04c07. x1 NCI_CGAP Lu5 Homo sapiens cDNA clone (MAGE : 1897260 3'similar to contains Alu repetitive'T 3633 8639 13645 0. 74 1. 9E-02 AI301183. 1 EST HUMAN element ; 3934 8933 13926 1. 5 1. 9E-02 AF141940. 1 NT Mycoplasma imitans VIhA1 precursor (vIhA1) and VIhA2 precursor (vIhA2) genes, partial cds _ 4070 9064 14053 1. 75 1. 9E-02 P09081 SW ISSPROT HOMEOTIC BICOID PROTEIN (PRD-4) 4070 9064 14054 1. 75 1. 9E-02 P09081 SW ISSPROT HOMEOTIC BICOID PROTEIN (PRD-4) tj46d04. x1 Soares NSF F8 9W OT_PA P S1 Homo sapiens cDNA clone IMAGE : 2144551 3'similar to 4410 9400 14385 2. 81 1. 9E-02 AI452999. 1 EST HUMAN confains Alu epe6tive element ; 4852 7405 12523 3. 15 1. 9E-02 AL161550. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fagment No. 80 hn52c06. x1 NCI CGAP Co17 Homo sapiens cDNA clone IMAGE : 3027274 3'similar to contains element 344 5396 10403 1. 36 1. 8E-02 AW 771104. 1 EST HUMAN MER29 repetitive element ; 1142 6146 11178 1. 42 1. 8E-02 X17664. 1 NT H. francisci mRNA for myelin basic protein (MBP) 2607 7569 12684 1. 45 1. 8E-02 AE004544. 1 NT Pseudomonas aeruginosa PA01, section 105 of 529 of the complete genome 3138 8154 0. 69 1. 8E-02 Af805829. 1 EST hiUMAN te52a09. x1 Soares NFL T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2090296 3' 3793 8796 13801 0. 91 1. 8E-02 AW 879122. 1 EST HUMAN MR1-OT0011-280300-009-g04 OT0011 Homo sapiens cDNA 4i : Page 50 of 209 Table 4 Single Exon Probes Expressed in HRI 100 Cells Probe Exon Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor Valut Value 3793 8796 13802 0.91 1.8E-02 AW879122. 1 =STHUMAN MR1-OT0011-280300-009-g040T0011 Homo sapiens cDNA 3970 8969 1. 08 1. 8E-02 AA861446. 1 EST_HUMAN ak24h04.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE: 1406935 3' 4306 9298 14284 1. 59 1. 8E-02 AW936363. 1 EST HUMAN QV4-DT0021-301299-071-b11 DT0021 Homo sapiens cDNA 4798 9782 14765 0. 1. 8E-02 O60810 SWISSPROT HYPOTHETICAL PROTEIN DJ845O24. 2 895 5913 10952 1. 21 1. 7E-02 BE394869. 1 EST HUMAN 601310626F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3632190 5' hf34a03.x1 Soares_NFL_T_BGC_S1 Homo sapiens cDNA clone IMAGE:2933740 3' similar to contains 1753 6747 11828 2. 15 1. 7E-02 AW573183. 1 EST HUMAN L1. t1 L1 repetitive element ; hf34a03.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:2933740 3' similar to contains 1753 6747 11829 2. 15 1. 7E-02 AW573183. 1 ESTHUMAN L1. ML1 repetitive etement ; 1833 6823 2. 15 1. 7E-02 AL163204. 2 NT Homo sapiens chromosome 21 segment HS21 C004 2054 7036 7. 25 1. 7E-02 AB004816. 1 NT Oryctolagus cuniculus mRNA for mitsugumin29, complete cds 2569 7532 1. 47 1. 7E-02 7657495 NT Homo sapiens putative Rab5 GDP/GTP exchange factor homologue (RABEX5), mRNA 2929 7948 12966 1. 09 1. 7E-02 AI147615. 1 EST HUMAN qb22a08. x1 Soares_pregnant uterus NbHPU Homo sapiens cDNA clone IMAGE : 1696982 3' hm45a04.x1 NCI_CGAP_RDF1 Homo sapiens cDNA clone IMAGE : 3015534 3'similar to contains 3433 8441 4. 78 1. 7E-02 AW827368. 1 EST HUMAN MER19. b1 MER19 repetitive element ; ac19f04. s1 Stratagene ovary (#937217) Homo sapiens cDNA clone IMAGE : 856927 3'similar to contains Alu 4045 9041 1. 04 1. 7E-02 AA669618. 1 EST HUMAN repetitive element ; contains element MER24 repetitive element ; 4077 9071 1. 86 1. 7E-02 R02506. 1 EST HUMAN ye86fO8. r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 124647 5' qm08g07. x1 NCI_CGAP_Lu5 Homo sapiens cDNA clone IMAGE : 1881276 3'similar to gb : X52359 ZINC 4332 9323 14309 1. 34 1. 7E-02 AI305279. 1 EST_HUMAN FINGER PROTEIN 30 (HUMAN) ; hf34a03. x1 Soares_NFL_T_GBC-S1 Homo sapiens cDNA clone IMAGE : 2933740 3'similar to contains 4396 9387 14370 1. 47 1. 7E-02 AW573183. 1 EST HUMAN L1. t1 L1 repetitive element ; 4576 9564 14552 1. 82 1. 7E-02 V00641. 1 NT Messenger RNA for anglerfish (Lophius americanus) somatostatin 11 4668 9653 6. 38 1. 7E-02 A1015076. 1 EST_HUMAN ov51e02.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1640858 3' 507 5542 1. 83 1. 6E-02 AL021929. 1 NT Mycobacterium tuberculosis H37Rv complete genome ; segment 13/162 1616 6613 116801. 13 1. 6E-02 Y18889. 1 NT Treponema maltophilum flaB2, flaB3 and fliD genes for flagellin subunit proteins and CAP protein homologue 2187 7166 12285 1. 01 1. 6E-02 Q64176 SW ISSPROT LIVER CARBOXYLESTERASE 22 PRECURSOR (EGASYN) (ESTERASE-22) 2187 7166 12286 1.01 1.6E-02 Q64176 SWISSPROT LIVER CARBOXYLESTERASE 22 PRECURSOR (EGASYN)(ESTERASE-22) 2572 7535 12652 1. 12 1. 6E-02 AA484872. 1 EST_HUMAN ne81d06.s1 NCI_CGAP_Ew1 Homo sapiens cDNA clone IMAGE : 910667 2622 7584 0. 95 1. 6E-02 AB014534. 1 NT Homo sapiens mRNA for KIAA0634 protein, partial cds 2951 7970 12987 0. 73 1.6E-02 AF11282. 1 INT Lasaea sp. isolate lBd cytochrome oxidase ni gene, partial cds ; mitochondrial gene for mitochondrial product, 3447 8455 13481 5.11 1.6E-02 AW850652.1 EST_HUMAN IL3-CT0219-160200-063-C07 CT0219 Homo sapiens cDNA Page 51 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top HIt SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession DAtabase Top Hit Descriptor NO NO: Signal BLAST E No. Value 3758 8761 13761 1.1 1.6E-02 AL163301. 2 NT Homo sapiens chromosome 21 segment HS21C101 Mus musculus major histocompatibility complex region NG27, NG28, RPS28, NADH oxidoreductase, NG29, KIFC1, Fas-binding protein, BING1, tapasin, RaIGDS-like, KE2, BING4, beta 1, 3-galactosyl transferase, and 4051 9047 1. 82 1. 6E-02 AF110520. 1 NT RPS18 genes, complete cds ; Sacm21 gene, partial 742 5765 23. 96 1. 5E-02 8923734 NT Homo sapiens transcription factor (HSA130894), mRNA 2081 7062 12171 2. 42 1. 5E-02 N39521. 1 EST HUMAN yv27b07. s1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 243925 3' 2110 7090 12204 1. 29 1.5E-02 AL161594. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 90 2987 8005 13017 1. 241. 5E-02 AJ006216. 1 NT Homo sapiens CACNA1 F gene, exons 1 to 48 2987 8005 13018 1.24 1.5E-02 AJ006216. 1 NT Homo sapiens CACNA1 F gene, exons 1 to 48 3641 8647 13654 0.88 1.5E-02 BF092942. 1 ESTHUMAN MR4-TN0115-080900-201-b12 TN0115 Homo sapiens cDNA 5006 9977 14952 0. 97 1. 5E-02 4503534 NT Homo sapiens eukaryotic translation initiation factor 4E (EIF4E) mRNA 414 5451 1. 25 1.4E-02 AE002230. 2 NT Chlamydophila pneumoniae AR39, section 58 of 94 of the complete genome 1101 6108 11138 3. 55 1. 4E-02 7705980 NT Homo sapiens NESH protein (LOC51225), mRNA 1237 6235 1.37 1.4E-02 U32800. 1 NT Haemophilus influenzae Rd section 115 of 163 of the complete genome 1279 6278 2. 38 1. 4E-02 U67779. 1 NT Xenopus laevis neurogenin related 1b (X-NGNR-1b) mRNA, complete cds 1486 6483 0. 96 1. 4E-02 AV723785. 1 EST HUMAN AV723785 HTB Homo sapiens cDNA clone HTBAHH11 5' 2926 7945 12962 0. 87 1. 4E-02 7657040 NT Homo sapiens down-regulated in metastasis (DRIM), mRNA Bifidobacterium longum Na+/H+ antiporter (nhaB), cytosine deaminase, and alpha-galactosidase (aglL) 3140 8156 13177 2. 22 1. 4E-02 AF160969. 2 NT genes, complete cds ; and N-acetylglucosamine/xylose repressor protein (nagC/xylR) gene, partial cds 3314 8325 13347 0. 73 1. 4E-02 AW074212. 1 EST HUMAN x09d09. x1 NCI CGAP GU1 Homo sapiens cDNA clone IMAGE : 2575793 3' 3404 8413 13439 6.12 1.4E-02 AL161586. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 82 3404 8413 13440 6. 12 1.4E-02 AL161586.2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 82 3572 8579 13585 10.27 1.4E-02 8996918 NT Mus musculus histocompatibility 2, complement component factor B (H2-Bf), mRNA 4361 9352 14331 7. 06 1. 4E-02 AW962688. 1 EST HUMAN EST374761 MAGE resequences, MAGG Homo sapiens cDNA 4361 9352 14332 7. 06 1. 4E-02 AW962688. 1 EST HUMAN EST374761 MAGE resequences, MAGG Homo sapiens cDNA 4726 9711 14695 6. 63 1.4E-02 BE733142. 1 ESTHUMAN 601567403F1NHMGC21 Homo sapiens cDNA clone IMAGE : 3842280 5' 4726 9711 14696 6. 63 1.4E-02 BE733142. 1 EST_HUMAN 601567403F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3842280 5' 1828 6818 1. 4 1. 3E-02 BE739263. 1 EST HUMAN 601556462F1 NIH MGC 58 Homo sapiens cDNA clone IMAGE : 3826335 5' 1915 6901 11994 1. 7 1. 3E-0 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21C001 2934 7953 12970 0. 87 1. 3E-02 M81725. 1 NT Oenothera berteriana NADH dehydrogenase subunit 2 (nad2) gene, exons 1-2 3141 8157 13178 1. 9 1.3E-02 BF697081. 1 EST_HUMAN 602129475F1 NIH_MGC_56 Homo sapiens cDNA clone IMAGE : 4286203 5' 3141 8157 13179 1. 9 1.3E-02 BF697081.1 EST_HUMAN 602129475F1 NIH_MGC_56 Homo sapiens cDNA done) IMAGE : 4286203 5' 3862 8864 1. 18 1. 3E-02 AF169288. 1 NT Mus musculus beta-sarcoglycan gene, complete cds : Page 52 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: No: Source Value Human gemine T-cell receptor beta chain TCRBV17S1A1T, TCRBV2S1, TCRBV10S1P, TCRBV29S1P, TCRBV19S1P, TCRBV15S1, TCRBV11S1A1T, HVB relic, TCRBV28S1P, TCRBV34S1, TCRBV14S1, 4771 9755 14742 1. 07 1. 3E-02 U66061. 1 NT TCRBV3S1, TCRBV4S1A1T, TRY4, TRY5, TRY6, TRY7, TRY8, TCRBD1, TCRBJ1S1, TCRBJ1S2, > zf65g01. r1 Scares retina N2b4HR Homo sapiens cDNA clone IMAGE : 381840 5'similar to contains element 353 5404 10414 4. 04 1. 2E-02 AA059299. 1 EST_HUMAN L1 repetitive element; 450 5487 10502 1. 52 1. 2E-02 P38898 SEISSPROT HYPOTHETICAL 17. 1 KD PROTEIN IN PUR5 3'REGION qd68e12. x1 Soares testisNHT Homo sapiens cDNA clone IMAGE : 1734670 3'similar to contains L1. t1 L1 729 5752 10773 5. 89 1. 2E-02 AI183522. 1 EST_HUMAN repetitive element; 2112 7092 12206 1. 27 1. 2E-02 AL163213. 2 NT Homo sapiens chromosome 21 segment HS21 C013 2115 7095 12209 1. 07 1. 2E-02 AV731704. 1 EST HUMAN AV731704 HTF Homo sapiens cDNA clone HTFBHG11 5' 2375 7347 12468 1. 15 1. 2E-02 AW172350. 1 EST_HUMAN xj37e09.x1 Scares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2659432 3' 2564 7347 12468 1.16 1.2E-02 AW172350.1 EST_HUMAN xj37e09.x1 Soares-NFL_T_GBC_S1 Homo sepiens cDNA clone IMAGE:2659432 3' 3028 8045 6.93 1.2E-02 AA075418.1 EST_HUMAN zm88e03.r1 Stratagene ovarian cancer (#937219) Homo sapiens cDNA clone IMAGE:545020 5' 3215 8230 13251 1. 97 1. 2E-02 R62805. 1 EST HUMAN yi11 b08. s1 Scares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 138903 3' 4729 9714 14699 0.95 1. 2E-02 6754367 NT Mus musculus interferon regulatory factor 5 (Irf5), mRNA Human hereditary haemochromatosis region, histone 2A-like protein gene, hereditary haemochromatosis 4761 9745 14731 3. 66 1. 2E-02 U91328. 1 NT (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds 4876 9855 1. 51 1. 2E-02 AB019786. 1 NT Cynops pyrrhogaster CpUbiqT mRNA, partial cds 4912 9891 14865 2. 45 1. 2E-02 AV731704. 1 EST HUMAN AV731704 HTF Homo sapiens cDNA clone HTFBHG11 5' 5025 9996 14969 1. 29 1. 2E-02 AF185576. 1 NT Mus muscuius P02yzinc finger transcription factor ODA-8 mRNA, complete cds 1250 6248 11289 1. 26 1. 1E-02 AA070364. 1 EST HUMAN zm69e11. s1 Stratagene neuroepithelium (#937231) Homo sapiens cDNA clone IMAGE : 530924 3' 1668 6664 11739 1. 43 1. 1 E-02 X75491. 1 NT H. sapiens LIPA gene, exon 4 1668 6664 11740 1. 43 1. 1E-02 X75491. 1 NT H. sapiens LIPA gene, exon 4 1987 6972 12076 3. 37 1. 1E-02 BF345263. 1 EST_HUMAN 602018037F1 NCI_CGAP_Bm67 Homo sapiens cDNA clone IMAGE : 4153808 5' 2808 7828 4. 07 1. 1E-02 N99523. 1 EST_HUMAN za40e05.r1 Soares fetal Ilver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 295040 5' tq95b10.x1 NCI_CGAP_Ov23 Homo sapiens cDNA clone IMAGE : 2216539 3'similar to SW : XPF_HUMAN 3444 8452 13479 3. 11 1. 1E-02 AI653508. 1 EST_HUMAN Q92889 DNA-REPAIR PROTEIN COMPLEMENTING XP-F CELL ; 3990 8988 0. 69 1. 1E-02 AW813796. 1 EST_HUMAN RC3-ST0197-10200-015-g11 ST0197 Homo sapiens cDNA 4683 9668 14650 2. 3 1. 1E-02 AL048383. 2 EST_HUMAN DKFZp586E0924_s1 586 (synonym : hute1) Homo sapiens cDNA clone DKFZp586E0924 7 5087 10073 8. 28 1. OE-02 AW846120. 1 EST HUMAN MR3-CT0176-111099-003-e10 CT0176 Homo sapiens cDNA 2497 7465 0. 95 1. OE-02 AA806389. 1 EST HUMAN oc22h08. s1 NCI CGAP GCB1 Homo sapiens cDNA clone IMAGE : 1350495 3' 3016 8033 13044 2.95 1.0E-02 BE835556.1 EST_HUMAN RC0-FN0025-250500-021-d02 FN0025 Homo sapiens cDNA Page 53 of 209 Table 4 Single Exon Probes Expresed in HRI 100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor ID NO: Signal BLAST E No. Value 3188 8204 13226 0. 98 1. 0E-02 BE968999. 1 EST_HUMAN 601649967R1 NIH_MGC_74 Homo sapiens cDNA clone IMAGE : 3933689 3' 4632 9617 14608 4. 68 1. OE-02 6753521 NT Mus musculus corticotropin releasing hormone receptor 2 (Crhr2), mRNA 4697 9682 14665 3. 4 1. OE-02 R96567. 1 EST HUMAN yq54h01. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 199633 5' 4855 9836 14810 1. 72 1. OE-02 L05632. 1 NT Human glycoprotein hormone alpha-subunit (GCA) gene, 5'flank wh42f09.x1 NCI_CGAP_Kid11 Homo sapiens cDNA done) IMAGE : 2383433 3'similar to contains element 882 5900 10941 2. 15 9. 0E-03 At796126. 1 EST HUMAN MER22 MER22 repetitive element ; 1245 6243 1.46 9. 0E-03 BE781889. 1 EST_HUMAN 601470242F1 NIH_MGC_67 Homo sapiens cDNA clone IMAGE : 3873346 5' 2331 7305 12425 1. 79 9. OE-03 AL161559. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 59 4828 9812 14793 1. 02 9. 0E-03 BE047949. 1 EST HUMAN tz44e10. y1 NCI CGAP Brn52 Homo sapiens cDNA clone IMAGE : 2291466 5' 5026 9997 14970 0. 98 9. OE-03 6753521 NT Mus musculus corticotropin releasing hormone receptor 2 (Crhr2), mRNA zh30e03. s1 Soares_pineal_gland_N3HPG Homo sapiens cDNA clone IMAGE : 413596 3'similar to contains 498 5534 2. 78 8. 0E-03 AA723007. 1 EST HUMAN Alu repetitive element ; 974 5990 1102352. 19 8. OE-03 AF106656. 1 NT Homo sapiens adenylosuccinate lyase gene, complete cds 2095 7076 12190 1. 39 8.0E-03 AL163283. 2 NT Homo sapiens chromosome 21 segment HS21C083 3280 8292 13317 0. 68 8. 0E-03 AJ131016. 1 NT Homo sapiens SCL gene locus 3591 8598 13603 1.23 8. OE-03 P32644 SW (SSPROT HYPOTHETICAL 127. 0 KD PROTEIN IN RAD24-BMH1 INTERGENIC REGION 3591 8598 13604 1.23 8. OE-03 P32644 ISSPROT HYPOTHETICAL 127. 0 KD PROTEIN IN RAD24-BMH1 INTERGENIC REGION 4134 9129 14112 0. 95 8. 0E-03 BE840049. 1 ESTHUMAN QVO-FN0181-140700-304-g10FN0181 Homo sapiens cDNA 4258 9252 14239 5.95 8.0E-03 BF363327. 1 EST HUMAN CM4-NN0119-300600-223-b05 NN0119 Homo sapiens cDNA 685 5709 10721 11. 47 7. OE-03 AF097183. 1 NT Cryptosporidium parvum HC-10 gene, complete cds 685 5709 10722 11. 47 7. 0E-03 AF097183. 1 NT Cryptosporidium parvum HC-10 gene, complete cds 962 5977 11011 11. 8 7. 0E-03 AF243376. 1 NT Glycine max glutathione S-transferase GST 21 mRNA, partial cds 1099 6106 11136 2. 63 7. OE-03 AV731712. 1 EST HUMAN AV731712 HTF Homo sapiens cDNA clone HTFAZF10 5' 1372 6369 11418 2. 08 7. 0E-03 AA668298. 1 EST_HUMAN ab79b09.s1 Stratagene fetal retina 937202 Homo sapiens cDNA clone IMAGE : 853145 3' 1473 6470 11528 4. 65 7. 0E-03 AW303599. 1 EST HUMAN xv2 b02. x1 Soares NFL_T_GBC S1 Homo sapiens cDNA clone IMAGE : 2813739 3' 2196 7765 12297 1. 36 7. OE-03 P04929 SWISSPROT HISTIDINE-RICH GLYCOPROTEIN PRECURSOR 3680 8685 13687 0. 92 7. OE-03 AW444463. 1 EST_HUMAN UI-H-BI3-abk-c-10-0-UI.s1 NCI_CGAP_Sub5 Homo sapiens cDNA clone IMAGE : 2733691 3' 3722 8726 13726 0. 78 7. OE-03 AF196344. 1 NT Rattus norvegicus neuronal nicotinic acetylcholine receptor subunit (Alpha10) mRNA, complete cds 4213 9206 1. 05 7. OE-03 U60086. 1 NT Dictyostelium discoideum multidrug resistance transporter/Ser protease (tagC) mRNA, complete cds xe34f09.x1 NCI_CGAP_Ut1 Homo sapiens cDNA clone IMAGE : 2609033 3' similar to TR : Q12987 Q12987 4404 9395 1. 12 7. 0E-03 AW117711. 1 EST HUMAN ACIDIC 82 KDA PROTEIN. ; 4468 9458 1.1 7.0E-03 AW630888.1 EST_HUMAN hh89a05.y1 NCI_CGAP_GU1 Homo sapiens cDNA clone IMAGE:2969936 5' Page 54 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Tap hit Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top HIt Descriptor NO : NO : ID NO: Singal BLAST E No. Source Value 4830 9814 .267 7.0E-03 AL163278. 2 NT Homo sapiens chromosome 21 segment HS21 C078 hd22a05. x1 Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE : 2910224 3'similar to 1221 6221 11263 9. 46 6. 0E-03 AW511148. 1 EST HUMAN SW : PXR_HUMAN 075469 ORPHAN NUCLEAR RECEPTOR PXR ; hd22a05. x1 Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE : 2910224 3'similar to 1221 6221 11264 9. 46 6. OE-03 AW511148. 1 EST_HUMAN SW:PXR_HUMAN @ 075469 ORPHAN NUCLEAR RECEPTOR PXR ; 2699 7656 12770 1. 02 6. OE-03 AF112374. 1 NT Danio rerio odorant receptor gene cluster 2820 7841 12857 4. 19 6. 0E-03 AA759135. 1 EST HUMAN ah78e11. s1 Soares testis NHT Homo sapiens cDNA clone 1321772 3' 2820 7841 12858 4. 19 6. OE-03 AA759135. 1 EST_HUMAN ah78e11.s1 Soares_testis_NHT Homo sapiens cDNA clone 1321772 3' 3173 8189 2. 51 6. OE-03 H75690. 1 EST HUMAN yr77h04. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 211351 5' 3232 8247 0. 85 6. 0E-03 AF190338. 1 NT Notoncus sp. cytochrome c oxidase subunit 11 gene, partial cds ; mitochondrial gene for mitochondrial product Fugu rubripes zinc finger protein, isotocin, fatty acid binding protein, sepiapterin reductase and vasotocin 3298 8309 13335 1. 31 6. 0E-03 U90880. 1 NT genes, complete cds Fugu rubripes zinc finger protein, isotocin, fatty acid binding protein, sepiapterin reductase and vasotocin 3298 8309 13336 1. 31 6. 0E-03 U90880. 1 NT genes, complete cds 3470 8478 1. 13 6. OE-03 W37985. 1 EST HUMAN zc13a11. r1 Soares_parathyroid tumor NbHPA Homo sapiens cDNA clone IMAGE : 322172 5' 3576 8583 13588 5. 07 6. 0E-03 BF510986. 1 EST HUMAN Ul-H-BI4-apm-c-06-0-Ul. s1 NCI CGAP Sub8 Homo sapiens cDNA clone IMAGE : 3087754 3' 3611 8618 136281. 29 6. 0E-03 BE077356. 1 EST HUMAN RC1-BT0606-260400-014-a07 BT0606 Homo sapiens cDNA 36908694 136961. 18 6. 0E-03 6754029 NT Mus musculus glucosamine-6-phosphate deaminase (Gnpi), mRNA 3850 8852 0. 86 6. 0E-03 BE250108. 1 EST_HUMAN 600942904F1 NIH_MGC_15 Homo sapiens cDNA clone IMAGE : 2959513 5' 4199 9192 1. 31 6. OE-03 N58946. 1 EST_HUMAN yy62h10.s1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE : 278179 3' 4239 9233 1. 27 6. OE-03 A1016833. 1 EST_HUMAN ov33c11.x1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE : 1639124 3' 4556 9544 14529 6. 94 6. 0E-03 AA324242. 1 EST-HUMAN EST27116 Cerebellum II Homo sapiens cDNA 5'end similar to EST containing Alu repeat 4950 9927 0. 98 6. OE-03 L34170. 1 NT Human germiline UBE1L gene similar to the gene for ubiquifin-activa6ng enzyme, exons 1-22 Chiamydia trachomatis partial ORFB ; aminoacyi-tRNA synthase, complete cds ; complete ORFA, and grpE- 661 5688 10696 1.81 5. 0E-03 L25105.1 NT like protein, complete cds Chlamydia trachomas partial ORFB ; aminoacyl-tRNA synthase, complete cds ; complete ORFA, and grpE- 661 5688 10697 1.81 5. OE-03 L25105. 1 NT like protein, complete cds Chlamydia trachomas partial ORFB ; aminoacyl-tRNA synthase, complete cds ; complete ORFA, and grpE- 662 5688 10696 2. 2 5.0E-03 L25105. 1 NT. like protein, complete cds Chlamydia trachomas partial ORFB ; aminoacyl-tRNA synthase, complete cds ; complete ORFA, and grpE- 662 5688 10697 2. 5. 0E43 L25105. 1 NT like protein, complete cds 1095 6102 11132 1. 24 5.0E-03 AJ010457. 1 NT Arabidopsis thaliana mRNA for DEAD box RNA helicase, RH3 2610 7572 12686 2. 3 5. 0E43 AB033006. 1 NIT Homo sapiens mRNA for KIAA1180 protein, partial cds Page 55 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top HIt ORE SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor NO : NO : ID NO: Signal BLAST E No. Source Value 2865 7885 12905 0. 69 5. OE-03 BE266057. 1 EST HUMAN 601194796F1 NIH MGC 7 Homo sapiens cDNA clone IMAGE : 3538799 5' 3063 8080 13094 3. 89 5. 0E-03 T87623. 1ESTHUMAN yc81f09. s1 Scares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 22395 3' 3081 8097 2. 71 5. OE-03 AL161491. 2 NT Arabidopsis thaliana DNA chromosome 4, contig fragment No. 3 3093 8109 13126 1. 36 5. 0E-03 R71794. 1 EST_HUMAN yj86g02.s1 Soares breast 2NbHBst Homo sapiens cDNA clone IMAGE : 155666 3' 3204 8219 0. 75 5. 0E-03 AJ297357. 1 NT Homo sapiens partial LiMD1 gene for UM domains containing protein 1 and KIAA0851 gene 3616 8623 13631 3. 67 5. 0E-03 AF147449. 2 NT Pseudomonas aeruginosa strain PA01 penicillin-binding protein 1B (ponB) gene, complete cds 3673 8678 13681 0. 67 5. 0E-03 U38914. 1 NT Citrus sinensis seed storage protein citrin mRNA, complete cds 3864 8866 1. 7 5. 0E-03 AA299675. 1 EST HUMAN EST12218 Uterus tumor I Homo sapiens cDNA 5'end 4179 8678 13681 0. 68 5. 0E-03 U38914. 1 NT Citrus sinensis seed storage protein citrin mRNA, complete cds 4476 9466 14445 0. 78 5. 0E-03 AJ131016. 1 NT Homo sapiens SCL gene locus 4580 9568 14557 1. 55 5. 0h AI752367. 1 EST_HUMAN cn15c02.x1 Normal Human Trabecular Bone Cells Homo sapiens cDNA clone NHTBC cn15c02 random 232 5295 10304 2. 13 4. OE-03 AW500196. 1 EST_HUMAN UI-HF-BN0-ack-h-04-0-UI.r1 NIH_MGC_50 Homo sapiens cDNA clone IMAGE : 3076831 5' 319 5374 10383 1. 88 4. OE-03 R46482. 1ESTHUMAN yg51e04. s1 Scares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 35988 3' 440 5477 10495 0. 67 4. 0E-03 P54675 SWISSPROT PHOSPHATIDYLINOSITOL 3-KINASE 3 (PI3-KINASE) (PTDINS-3-KINASE) (PI3K) 598 5629 10628 3. 1 4. OE-03 AA939339. 1 EST_HUMAN on75g12.s1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 1562566 3' 866 5884 10927 1. 7 4. OE-03 R46482. 1 EST_HUMAN yg51e04.s1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 35988 3' 900 5918 1 2. 96 4. OE-03 AW749101. 1 EST HUMAN RC3-BT0333-110100-012-f01 BT0333 Homo sapiens cDNA 1133 6138 11169 24. 08 4. 0E-03 AA099777. 1 EST HUMAN zi81aO8. r1 Stratagenecolon (#937204) Homo sapienscDNA clone IMAGE : 510998 5' 1151 6155 11188 1. 42 4. OE-03 AW794740. 1 ESTHUMAN RC6-UM0014-170400-023-G01UM0014 Homo sapiens cDNA 1284 6283 11325 1. 02 4. OE-03 AA284374. 1 EST_HUMAN zs59a01.r1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE : 701736 5' 1551 6548 1. 29 4. OE-03 AV708305. 1 EST HUMAN AV708305 ADC Homo sapiens cDNA clone ADCAKB06 5' 1704 6699 11775 1. 99 4. OE-03 U33472. 1 NT Rattus norvegicus type 1 astrocyte and dfaetory-iimbic associated protein AT1-46 mRNA, complete cds 1968 6953 12058 7. 06 4. 0E-03 AA099777.1 EST_HUMAN zi81a08.r1 Stratagene colon (#937204) Homo sapiens cDNA clone IMAGE : 510998 5' 2186 7165 1. 43 4. 0E-03 BE410556.1 EST_HUMAN 601304161F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3638510 5' 2215 7192 12314 1. 14 4. OE-03 AW794740. 1 EST_HUMAN RC6-UM0014-170400-023-G01 UM0014 Homo sapiens cDNA Homo sapiens X28 region near ALD locus containing dual specificity phosphatase 9 (DUSP9), ribosomal protein Ll8a (RPL18a), Ca2+/Calmodulin-dependent protein kinase I (CAMKI), creatine transporter (CRTR), 2498 7466 12580 1. 56 4. OE-03 U52111. 2 NT CDM protein (CDM), adrenoleukodystrophy protein > Homo sapiens X28 region near ALD locus containing dual specificity phosphatase 9 (DUSP9), ribosomal protein L18a (RPL18a), Ca2+/Calmodulin-dependent protein kinase I (CAMKI), creatine transporter (CRTR), 2498 7466 12581 1. 56 4. OE-03 U52111. 2 NT CDM protein (CDM), adrenoleukodystrophy protein > 2619 7581 12691 2.52 4. 0E-03 AJ277365. 1 NT Homo sapiens polyglutamine-containing C140RF4 gene Page 56 of 209 Table 4 Sinple Exon Probes Exaressed in HBL100 Cells Probe Similar ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value 2619 7581 12692 2.52 4.0E-03 AJ277365.1 NT Homo sapiens polyglutamine-containing C14ORF4 gene 2624 7585 12695 1. 04 4. OE-03 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21C084 3154 8170 13191 1. 06 4. OE-03 BE154134. 1 ESTHUMAN PM1-HT0340-151299-003-h08HT0340 Homo sapiens cDNA 3154 8170 13192 1. 06 4. OE-03 BE154134. 1 EST'HUMAN PM1-HT0340-151299-003-h08 HT0340 Homo sapiens cDNA 3452 8460 13486 0.92 4.0E-03 AW188426. 1 EST_HUMAN xj98f04.x1 NCI_CGAP_Co18 Homo sapiens cDNA clone IMAGE : 2665279 3' 3452 8460 12487 0.92 4.0E-03 AW188426. 1 EST_HUMAN xj98f04.x1 NCI_CGAP_Co18 Homo sapiens cDNA clone IMAGE : 2665279 3' 3815 8548 13556 1. 02 4. OE-03 Q13606 SW ISSPROT OLFACTORY RECEPTOR 511 (OLFACTORY RECEPTOR-LIKE PROTEIN OLF1) 3889 8889 1. 73 4.0E-03 AJ011712. 1 NT Homo sapiens TNNT1 gene, exons 1-11 (and joined CDS) ab18a08. x5 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE : 841142 3'similar to contains Alu 4480 9470 14451 1.08 4.0E-03 AI732754. 1 EST HUMAN repetitive element ; 370 5419 10434 2. 62 3. OE-03 AF011920. 1 NT Homo sapiens protein kinase CK2 catalytic subunit alpha gene, exon 1 868 5886 10928 5. 77 3. OE-03 AF011920. 1 NT Homo sapiens protein kinase CK2 catalytic subunit alpha gene, exon 1 nc73c05.s1 NCI_CGAP_Pr2 Homo sapiens cDNA clone IMAGE : 782984 similar to contains Alu repetitive 1621 6618 11684 2. 72 3. 0E-03 AA468110. 1 EST HUMAN element ; 2231 7208 7. 09 3.0E-03 Z32521. 1 NT S. cereale (cv. Halo) mRNA for triosephosphate isomerase 2232 7209 12323 0. 92 3. OE-03 U46858. 1 NT Mus musculus intestinal trefoil factor gene, partial cds 2232 7209 12324 0. 92 3.0E-03 U46858.1 NT Mus musculus intestinal trefoil factor gene, partial cds 2923 7942 0. 93 3. OE-03 Y09006. 1 NT Arabidopsis thaliana rpoMt gene 3009 8026 13038 4. 49 3. OE-03 BE379296. 1 EST HUMAN 601237982F1 NIH MGC 44 Homo sapiens cDNA clone IMAGE : 3609933 5' 3078 8094 13108 3. 09 3.0E-03 AW802687. 1 EST_HUMAN IL2-UM0076-240300-056-D03 UM0076 Homo sapiens cDNA 3333 8343 13361 2. 06 3. OE-03 U34606. 1 NT Mus musculus alpha-1 (XVIII) collagen (COL18A1) gene, exon 1 and 2 3342 8351 7. 49 3. OE-03 Y12500. 1 NT C. elegans samdc gene 3870 8871 13876 7. 06 3.0E-03 AV762392, 1 EST_HUMAN AV762392 MDS Homo sapiens cDNA clone MDSBSG01 5' 3870 8871 13877 7. 05 3. OE-03 AV762392. 1 EST HUMAN AV762392 MDS Homo sapiens cDNA clone MDSBSG01 5' 3925 8925 13915 1.6 3.0E-03 AI792278. 1 EST_HUMAN ah04f09. y5 Gessler Wilms tumor Homo sapiens cDNA clone IMAGE : 1155689 5' 4027 9023 1. 04 3.0E-03 Z32521. 1 NT S.cereale (cv. Halo) mRNA for triosephosphate isomerase 4271 9264 14254 4. 26 3.0E-03 AJ011432. 1 NT Rattus norvegicus gdnf gene 4338 9329 0. 68 3.0E-03 BE348739. 1 EST HUMAN ht68g08. x1 NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3151934 3' 4387 9378 14359 5. 15 3.0E-03 AI536141. 1 EST HUMAN xu8. P1Q. H3 conorm Homo sapiens cDNA 3' ab18aO8. x5 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE : 841142 3'similar to contains Alu 4686 9671 14653 2. 19 3. 0E-03 AI732754. 1 EST HUMAN repetitive element ; 4705 9690 14673 6. 19 3.0E-03 BE787945.1 EST_HUMAN 601482715F1 NIH_MGC_68 Homo sapiens cDNA clone IMAGE : 3885483 5' 4968 9945 14922 0.92 3.0E-03 4506414 NT Homo sapiens RAP1, GTPase activating protein 1 (RAP1GA1) mRNA 4968 9945 14923 0. 92 3.0E-03 4506414 NT Homo sapiens RAP1, GTPase activating protein 1 (RAP1 GA1) mRNA Page 57 of 209 Table 4 Sing (e Exon Probes Expressed in HBL100 Cells Most Similar To Hit SEQ D SEQ ID F SEQ Eression (Top) Nit Top Hit Acession paase Top Hit Descriptor NO : NO : ID NO : Signal BLAST E No. grce Value Value 5014 9985 14961 0. 98 3. OE-03 S52213. 1 NT CD11 b=leukocyta integrin alpha chain [human, Genomic, 104 nt, sagment 23 of 31] 511 5546 10550 0. 69 2. OE-03 Q04652 SW ISSPROT RING CANAL PROTEIN (KELCH PROTEIN) 511 5546 10551 0. 69 2. OE-03 Q04652 SW ISSPROT RING CANAL PROTEIN (KELCH PROTEIN) 777 7731 90. 87 2. OE-03 T70874. 1 EST HUMAN yd15h03. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 108341 5' 1346 6343 11394 1. 92 2. OE-03 M20783. 1 NT Human alpha-2-plasmin inhibitor gene, exans 6 and 7 1348 6345 11396 1. 98 2. OE-03 AA661605. 1 EST HUMAN nu86f01. s1 NCI CGAP AIv1 Homo sapiens cDNA clona MAGE : 1217593 1357 6354 11404 8. 74 2. OE-03 AF284446. 1 NT Homo sapiens tumor-related protein DRC2 (DRC2) gene, complete cds PLATELET ENDOTHELIAL T'ETRASPAN ANTIGEN 3 (PETA-3) (GP27) (MEMBRANE GLYCOPROTEIN 1458 6455 11514 4. 39 2. OE-03 P48509 SWISSPROT SFA-1) (CD151 ANTIGEN) Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehlers-Danlos syndroma 1485 6482 11537 1. 7 2. OE-03 4557836 NT type VI) (PLOD) mRNA Homo sapiens procollagen-lysine, 2-oxoglutarate 5-dioxygenase (lysine hydroxylase, Ehlers-Danlos syndrome 1485 6482 11538 1. 7 2. OE-03 4557836 NT type VI) (PLOD) mRNA 1556 6553 4. 37 2. OE-03 P29400 SW ISSPROT COLLAGEN ALPHA 5 (IV) CHAIN PRECURSOF2 1734 6729 11806 1. 16 2. OE-03 AA450138. 1 EST HUMAN nc42a10. r1 Soares total fetus Nb2HF8 9w Homo sapiens cDNA clone IMAGE : 789114 5' 1949 6935 12036 1. 19 2. OE-03 AF302691. 1 NT Mus musculus myelin eression factor-3-like protein gene, partial cds 2188 7167 12287 1. 03 2. OE-03 AL163302. 2 NT Homo sapiens chromosome 21 segment HS21 C102 2504 7472 3. 15 2. OE-03 AW 137782. 1 EST HUMAN UI-H-BI1-adi-g-10-0-Ul. s1 NCI CGAP_Sub3 Homo sapiens cDNA clone IMAGE : 2717010 3' 3332 8342 13360 5. 57 2. OE-03 AA450138. 1 EST HUMAN ac42a10. 1 Soares total fetus Nb2HF8 9w Homo sapiens cDNA clone IMAGE : 789114 5' 3338 8347 13365 0. 86 2. OE-03 BF568955. 1 EST HUMAN 602183960T1 NIH MGC 42 Homo sapiens cDNA clone IMAGE : 4300070 3' : _ H. sapiens DMA, DMB, HLA-Z1, IPP2, LMP2, TAP1, LMP7, TAP2, DOB, DQB2 and RING8, 9, 13 and 14 3582 8589 13593 5. 61 2. OE-03 X87344. 1 NT genes' 3997 8993 13980 2. 35 2. OE-03 P03374 SW ISSPROT ENV POLYPROTEIN [CONTAINS : COAT PROTEIN GP52 ; COAT PROTEIN GP36] 4099 9093 8. 68 2. OE-03 U68491. 1 NT Rattus norvegicus 5-hydroxytryptamine7 recepta gene, partial cds. 4303 9295 1. 17 2. OE-03 AW297380. 1 EST HUMAN UI-H-BWO-air-g-03-0-UI. s1 NCI CGAP Sub6 Homo sapiens cDNA clone IMAGE : 2730413 3' 4307 9299 14285 0. 92 2. OE-03 A1064746. 1 EST HUMAN HA0507 Human fetal liver cDNA library Homo sapiens cDNA 4415 9405 14390 2. 22 2. OE-03 L42512. 1 NT Drosophila melanogaster shortsighted class 2 (shs) mRNA, complete cds 4415 9405 14391 2. 22 2. OE-03 L42512. 1 NT Dosophila melanogaster shortsighted class 2 (shs) mRNA, complete cds 4573 9561 1. 9 2. OE-03 R87773. 1 EST HUMAN yo45e02. s1 Soares adult brain N2b4HB55Y Homo sapiens cDNA cione IMAGE : 180890 3' Homo sapiens X-linked anhidroitic ectodermal dyspiasia protein gene (EDA), exon 2 and flanking repeat 4861 9842 14814 0. 94 2. OE-03 AF003528. NT regians 436 5474 10490 1. 26 1. OE-03 H96471. 1 EST HUMAN yt98c08. r1 Soaresineal gland N3HPG Homo sapiens cDNA clone IMAGE : 232334 5' as70b08. x1 Barstead cdon HPLRB7 Homo sapiens cDNA clone IMAGE : 2334039 3'similar to TR : Q13825 = 819 5839 10876 1. 31 1. OE-03 AI720263. 1 EST HUMAN Q13825 AU-BINDING PROTEINIENOYL-COA HYDRATASE. ; Page 58 of 209 Tab) e 4 Single Exon Probes Expressed in HBL100 Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SET ID SEQ ID ID NO : Signal BLAST E No. Source Top Hit Descriptor Value as70b08. x1 Barstead colon HPLRB7 Homo sapiens cDNA clone IMAGE : 2334039 3'similar to TR : Q13825 819 5839 10877 1. 1. 0E-03 AI720263. 1 EST_HUMAN Q13825 AU-BINDING PROTEIN/ENOYL-COA HYDRATASE. ; 1078 6085 11114 3. 35 1. OE-03 AI865788. 1 EST_HUMAN wk86a06.x1 NCI_CGAP_Pan1 Homo sapiens cDNA clone IMAGE : 2422258 3' 1098 6105 11135 1. 17 EST_HUMAN wx93e10.x1 NCI_CGAP_Mel15 Homo EST_HUMAN cDNA clone IMAGE Homo 2551242 CDNA wd86aO1. x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 2338440 3'similar to contains Alu 1148 6152 11184 4. 08 1. OE-03 Al692616. 1 EST HUMAN repetitive element ; 1977 6962 12069 2. 86 1. OE-03 P47808 SWISSPROT HIGH MOLECULAR WEIGHT FORM OF MYOSIN I (HMWMI) 2093 7074 12188 4. 54 1. 0E-03 AJ131016. 1 NT Homo sapiens SCL gene locus 2911 7930 12949 1. 8 1. OE-03 AB033117. 1 NT Homo sapiens mRNA for KIAA1291 protein, partial cds CARBONIC ANHYDRASE Vl PRECURSOR (CARBONATE DEHYDRATASE VI) (CA-VI) (SECRETED 3118 8134 13153 2.17 1. 0E-03 P18915 SWISSPROT CARBONIC ANHYDRASE) (SALIVARY CARBONIC ANHYDRASE) CARBONIC ANHYDRASE VI PRECURSOR (CARBONATE DEHYDRATASE VI) (CA-VI) (SECRETED 3118 8134 13154 2. 17 1. 0E-03 P18915 SWISSPROT CARBONIC ANHYDRASE) (SALIVARY CARBONIC ANHYDRASE) 3228 8243 13265 0. 75 1. 0E-03 P08547 SWISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 3466 8474 13498 0. 76 1. OE-03 U68061. 1 NT Human MUC2 gene, promoter region 3466 8474 13499 0. 76 1. 0E-03 U68061. 1 NT Human MUC2 gene, promoter region 3581 8588 1. 51 1. 0E-03 AB044400. 1 NT Homo sapiens SVMT gene for synaptic vesicle monoamine transporter, exons 14, 15 3831 8833 13840 0. 76 1. 0h Z49649. 1 NT S. cerevisiae chromosome X reading frame ORF YJR149w 4312 9304 14288 5. 29 1. OE-03 BE939162. 1 ESTHUMAN RC1-TN0128-160800-021-g01TN0128 Homo sapiens cDNA TCBAP1D4909 Pediatric pre-B cell acute lymphoblastic leukemia Baytor-HGSC projectTCBA Homo 4349 9340 14321 4. 94 1. OE-03 BE246536. 1 EST HUMAN sapiens cDNA clone TCBAP4909 4528 9518 14505 0. 91 1. OE-03 U29449. 1 NT Caenorhabditis elegans spliced leader RNA (SL3 alpha), (SL4), and (SL5) genes 4677 9662 14644 1. 69 1. OE-03 A1073485. 1 EST HUMAN ov45c04. x1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 1640262 3' 4677 9662 14645 1. 69 1. 0E-03 Alto73485. 1 EST HUMAN ov45c04. x1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE : 1640262 3' 4678 9663 5.57 1.0E-03 BE154067. 1 EST HUMAN PMO-HT0339-200400-010-D02 HT0339 Homo sapiens cDNA 4905 9884 14855 8. 4 1. OE-03 046409 SW ISSPROT APOLIPOPROTEIN A-IV PRECURSOR (APO-AIV) ai61c12. s1 SoarestestisNHT Homo sapiens cDNA clone 1375318 3'similar to SW : AATC CHICK P00504 4896 9875 14843 1. 32 9. OE-04 AA815400. 1 EST HUMAN ASPARTATE AMINOTRANSFERASE, CYTOPLASMIC ; s 4056 9050 4. 35 8. OE-04 P08547 SWISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 4619 9604 14592 2. 42 8. OE-04 U29185. 1 NT Homo sapiens prion protein (PrP) gene, complete cods 2335 7309 12430 1. 75 7. OE-04 U29185. 1 NT Homo sapiens prion protein (PrP) gene, complete cds 2642 7602 12715 1.09 7.0E-04 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 3207 8222 13244 1.03 7.0E-04 4885170 NT Homo sapiens chromosome X open reading frame 6 (CXORF6) mRNA 3853 8855 13860 1.51 6. OE-04 AI862525. 1 EST_HUMAN wj15a11.x1 NCI_CGAP_Kid12 Homo sapiens cDNA clone IMAGE : 2402876 3' Page 59 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Vaque 4060 9054 14041 3. 01 6. 0E-04 U45983. 1 NT Homo sapiens CCR8 chemokine receptor (CMKBR8) gene, complete cds 643 5671 10675 10. 72 5. 0E-04 O10341 SWISSPROT HYPOTHETICAL 29. 3 KD PROTEIN (ORF92) 1472 6469 1. 4 5. 0E-04 AW851844. 1 EST HUMAN QVO-CT0225-021099-030-a07 CT0225 Homo sapiens cDNA nk27e11. s1 NCI CGAP Co11 Homo sapiens cDNA clone IMAGE : 1014764 3'similar to contains Alu 3329 8339 13357 1. 21 5. 0E-04 AA548931. 1 EST HUMAN repetitive element ; 665 5691 10700 1. 07 4. 0h U32748. 1 NT Haemophilus influenzas Rd section 63 of 163 of the complete genome as70b08. x1 Barstead colon HPLRB7 Homo sapiens cDNA clone IMAGE : 2334039 3'similar to TR : Q13825 837 5856 10896 1. 34 4. 0E-04 AI720262.1 EST_HUMAN Q13825 AU-BINDING PROTEIN/ENOYL-COA HYDRATASE.; as70b08. x1 Barstead colon HPLRB7 Homo sapiens cDNA clone IMAGE : 2334039 3'similar to TR : Q13825 837 5856 10897 1. 34 4. 0E-04 AI720263. 1 EST HUMAN Q13825 AU-BINDING PROTEINIENOYL-COA HYDRATASE. ; 1437 6434 11491 2. 18 4. 0E-04 AW753356. 1 EST_HUMAN RC3-CT0254-130100-023-fO1 CT0254 Homo sapiens cDNA 2030 7013 12122 1. 19 4. 0E-04 AL163278. 2 NT Homo sapiens chromosome 21 segment HS21C078 2075 7057 0. 94 4. 0E-04 AL046704. 1 EST_HUMAN DKFZp434D059_r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434D059 5' 2554 7519 12636 2. 01 4. 0E-04 096615 SWISSPROT SERICIN-2 (SILK GUM PROTEIN 2) 3091 8107 13123 3. 3 4. 0E-04 AF281074. 1 NT Homo sapiens neuropilin 2 (NRP2) gene, complete cds, alternatively spliced 3776 8779 1. 12 4. 0E-04 AL163267. 2 NT Homo sapiens chromosome 21 segment HS21 C067 nh10a10. s1 NCI_CGAP_C01 Homo sapiens cDNA clone IMAGE : 951930 3'similar to gb : M21121 T-CELL 4196 9189 14169 2. 79 4. 0E-04 AA576331. 1 EST-HUMAN SPECIFIC RANTES PROTEIN PRECURSOR (HUMAN) ; nh10a10. s1 NCI_CGAP_C01 Homo sapiens cDNA clone IMAGE : 951930 3'similar to gb : M21121 T-CELL 4196 9189 14170 2. 79 4. 0E-04 AA576331. 1 EST_HUMAN SPECIFIC RANTES PROTEIN PRECURSOR (HUMAN) ; 4407 9398 14382 2. 39 4. 0E-04 AA086324. 1 ESTHUMAN zn61c08. s1 Stratagene muscle 937209 Homo sapiens cDNA clone IMAGE : 562670 3' 4915 9893 14867 3.42 4.0E-04 BE560660. 1 EST_HUMAN 601345895F1 NIH_MGC_8 Homo sapiens cDNA clone IMAGE:3678910 5' yy78b10.s1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE : 279643 3'similar to 5019 9990 14965 1. 02 4. 0E-04 N48313. 1 EST_HUMAN contains Alu repetitive element ; 155 5221 10233 2. 94 3. OE-04 AL119426. 1 EST HUMAN DKFZp761J221 r1 761 (synonym : hamy2) Homo sapiens cDNA clone DKFZp761J221 5' 195 5259 10272 1. 5 3. 0E-04 P49259 SWISSPROT 180 KD SECRETORY PHOSPHOLIPASE A2 RECEPTOR PRECURSOR (PLA2-R) 869 5887 10929 2. 14 3. 0E-04 U83991. 1 NT Human short chain acyi CoA dehydrogenase gene, exons 1 and 2 1803 6794 11884 1. 69 3. 0E-04 AI262100. 1 EST HUMAN qz28d03. y1 NCI CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2028197 5' 1817 6807 2. 03 3. 0E-04 AI399674. 1 EST HUMAN th23a02. x1 NCI_CGAP Pr28 Homo sapiens cDNA clone IMAGE : 2119082 3' 3236 8250 13271 4. 06 3. 0E-04 P25147 SWISSPROT INTERNALIN B PRECURSOR 3857 8859 13865 3. 31 3. 0E-04 P49448 SWISSPROT GWTAMATE DEHYDROGENASE 2 PRECURSOR (GDH) 3942 8940 1. 31 3. 0E-04 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1/2 3976 8974 1. 09 3. OE-04 BE140609. 1 EST HUMAN RCO-HT0014-310599-028 HT0014 Homo sapiens cDNA 4671 9656 5.1 3. 0E-04 DE153778. 1 EST HUMAN PMO-HT0339-190200-007-g12 HT0339 Homo sapiens cDNA T Page 60 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells i Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ NO : Signal BLAST E No. Source Top Hit Descriptor Value Homo sapiens SCG10 like-protein, helicase-like protein NHL, M68, and ADP-ribosylation factor related 175 5239 10250 1. 52 2. 0E-04 AF217796. 1 NT protein 1 (ARFRP1) genes, complete cds 475 5511 105241 5.11 2.0E-04 AU146707. 1 EST HUMAN AU146707 HEMBB1 Homo sapiens cDNA clone HEMBB1001253 3' 896 5914 10953 6. 36 2. OE-04 M86524. 1 NT Human dystrophin gene 896 5914 10954 6.36 2.0E-04 M86524.1 NT Human dystrophin gene qh98e11. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 1855052 3'similar to contains 1160 6164 3. 63 2. 0E-04 AI286021. 1 EST HUMAN MER3. b2 MER3 repetitive element ; 1167 6170 2. 07 2. OE-04 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 1796 6787 0. 97 2. OE-04 AF224268. 1 NT Mus musculus 5'flanking region of Pitx3 gene zu39b05. s1 Soares ovary tumor NbHOT Homo sapiens cDNA clone IMAGE : 740337 3'similar to contains Alu 2120 7100 1. 21 2. OE-04 AA478980. 1 EST HUMAN repetitive element Human germline T-cell receptor beta chain TCRBV17S1A1T, TCRBV2S1, TCRBV10S1P, TCRBV29S1P, TCRBV19S1P, TCRBV15S1, TCRBV11S1A1T, HVB relic, TCRBV28S1P, TCRBV34S1, TCRBV14S1, 2500 7468 12583 3. 15 2. 0E-04 U66061. 1 NT TCRBV3S1, TCRBV4S1A1T, TRY4, TRY5, TRY6, TRY7, TRY8, TCRBD1, TCRBJ1S1, TCRBJ1S2, > 2918 7937 12954 1.18 2. 0E-04 A1124529. 1 EST HUMAN am58c09. x1 Johnston frontal cortex Homo sapiens cDNA clone IMAGE : 1539760 3' 3260 8273 13296 1. 1 2.0E-04 5174736 NT Homo sapiens tubulin, beta, 4 (TUBB4) mRNA 3351 8360 13376 2. 67 2. 0E-04 BE082317. 1 EST HUMAN QV2-BT0636-070500-194-b07 BT0636 Homo sapiens cDNA 3381 8389 13412 0. 91 2. OE-04 U34374. 1 NT Human tyrosine kinase TXK (txk) gene, exons 9 and 10 3816 8818 13825 1. 04 2. 0E-04 AW978441. 1 EST HUMAN EST390550 MAGE resequences, MAGP Homo sapiens cDNA 4020 9016 5. 76 2. 0E-04 U01029. 1 NT Phaseolus vulgaris nitrate reductase (PVNR2) gene, complete cds 4534 9524 14511 1. 51 2. 0E-04 H96265. 1 EST_HUMAN yu01e11.r1 Soares_pineal_gland_N3HPG Homo sapiens cDNA clone IMAGE : 232556 5' 4534 9524 14512 1. 51 2. 0E-04 H96265. 1 EST_HUMAN yu01e1.r1 Soares_pineal_gland_N3HPG Homo sapiens cDNA clone IMAGE : 232556 5' 4651 9636 1. 39 2. 0E-04 U09226. 1 NT Gallus gallus proteasome 28 kDa subunit homdog mRNA, complete cds 4891 9870 14838 1.87 2.0E-04 AB037997.1 NT Danio rerio hagoromo gene, exons 1 to 6, partial cds yx26c09.s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 262864 3'similar to contains 758 5779 10806 1. 09 1. 0E-04 H99646. 1 EST HUMAN Ll. t1 L1 repetitive element ; RETROVIRUS-RELATED POL POLYPROTEIN [CONTAINS : REVERSE TRANSCRIPTASE ; 1058 6067 11097 1. 86 1. OE-04 P11369 SWISSPROT ENDONUCLEASE] 1097 6104 11133 3. 05 1. 0E-04 AW013847. 1 EST_HUMAN UI-H-BI0-aab-e-09-0-UI.s1 NCI_CGAP_Sub1 Homo sapiens cDNA clone IMAGE : 2708825 3' 1097 6104 11134 3. 05 1. 0E-04 AW013847. 1 EST HUMAN UI-H-BIO-aab-e-09-0-Ul. s1 NC (CGAP Sub1 Homo sapiens cDNA clone IMAGE : 2708825 3' 1312 6309 3.3 1.0E-04 U62918.1 NT Anguille anguilla doparmine D1A1 receptor (d1A1) gene, complete cds Page 61 of 209 Table 4 Single Exon Probes Expressed in HRI 100 Cells Probe Exon Most Similar SEO ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit NO N NO : Signal BLAST E No. Database Top Hit Descriptor Value Kapos's sarcoma-associated herpesvirus ORF 68 gene, partial cds ; and ORF 69, kaposin, v-FLIP, v-cyclin, latent nuclear antigen, ORF K14, v-GPCR, putative phosphoribosylformylglycinamidine synthase, and LAMP 1588 6585 11646 3. 97 1. OE-04 AF148805. 1 NT (LAMP) genes, complete cds Kaposi's sarcoma-associated herpesvirus ORF 68 gene, partial cds ; and ORF 69, kaposin, v-FLIP, v-cyclin, latent nuclear antigen, ORF K14, v-GPCR, putative phosphoribosylformylglycinamidine synthase, and LAMP 1588 6585 11647 3. 97 1. OE-04 AF148805. 1 NT (LAMP) genes, complete cds 1824 6814 11908 1. 7 1. OE-04 AB048342. 1 NT Equus caballus DNA, chromosome 24q14, microsatellite TKY36 3211 8226 13248 0.96 1.0E-04 Q62203 SWISSPORT SPLICEOSOME ASSOCIATED PROTEIN 62 (SAP 62) (SPLICING FACTOR 3A SUBUNIT 2) (SF3A66) tj01f11.x1 NCI_CGAP_Gas4 Homo sapiens cDNA clone IMAGE:2140296 3' simlar to contains Alu repetitive 3651 8657 13663 0. 72 1. OE-04 AI440282. 1 EST HUMAIN element ; 3948 8946 13936 1. 86 1. 0E-04 M14042. 1 NT Mouse alpha 1 type-IV collagen mRNA 3967 8966 13955 1.09 1.0E-04 AV647727. 1 EST HUMAN AV647727 GLC Homo sapiens cDNA clone GLCBBD04 3' 4925 9902 14878 1. 64 1. 0E-04 7662015 NT Homo sapiens KIAA0237 gene product (KIAA0237), mRNA 4925 9902 14879 1.64 1. OE-04 7662015 NT Homo sapiens Kl0237 gene product (KIAA0237), mRNA 689 5713 10727 1. 84 9. 0E-05 AA718933. 1 ESTHUMAN ah45c11. s1 SoarestestisNHT Homo sapiens cDNA clone 1292468 3' 811 5832 10865 1. 19 8. 0E-05 AJ251646. 1 NT Pisum sativum mRNA for beta-1, 3 glucanase (gns2 gene) 853 5872 12. 78 8. 0E-05 AJ251646. 1 NT Pisum sativum mRNA for beta-1, 3 glucanase (gns2 gene) 4353 9344 14323 0. 67 8. 0E-05 AW044605. 1 EST_HUMAN wy78a04.x1 Soares_NSF_F8_9W_OT_PA_P-S1 Homo sapiens cDNA clone IMAGE : 2554638 3' 345 5397 10404 11. 8 7. 0E-05 AW847445. 1 EST HUMAN RC3-CT0208-220999-011-E04 CT0208 Homo sapiens cDNA 345 5397 10405 11.8 7.0E-05 AW847445. 1 EST HUMAN RC3-CT0208-220999-011-E04 CT0208 Homo sapiens cDNA 562 5596 10595 1. 24 7. 0E-05 L49075. 1 EST HUMAN HUM072014F Human fovea cDNA Homo sapiens cDNA clone EST HFD072014 562 5596 10596 1. 24 7. OE-05 L49075. 1 EST_HUMAN HUM072014F Human fovea cDNA Homo sapiens cDNA clone EST HFD072014 PROBABLE GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE, MITOCHONDRIAL PRECURSOR 1038 6048 11078 1. 43 7 OE-05 Q22949 SWISSPROT (GPAT) 2646 7606 12718 2. 46 7. 0E-05 AL163278. 2 NT Homo sapiens chromosome 21 segment HS21C078 3085 8101 13116 4. 38 7. 0E-05 AB009080. 1 NT Dictyostelium discoideum gene for TRFA, complete cds 3617 8624 1. 24 7. 0E-05 AI432413. 1 ESTHUMAN tg73c09. x1 SoaresNhHMPuS1 Homo sapiens cDNA clone IMAGE : 2114416 3' 3943 8941 0. 72 7. 0E-05 AF111167. 2 NT Homo sapiens jun dimerization protein gene, partial cds ; cfos gene, complete cds ; and unknown gene 4247 9241 14225 1. 61 7. 0E-05 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21 C001 4769 9753 14741 0. 66 7. 0E-05 9845300 NT Rat cytomegalovirus Maastricht, complete genome 1976 6961 12067 1. 6 6. 0E-05 4885170 NT Homo sapiens chromosome X open reading frame 6 (CXORF6) mRNA 1976 6961 12068 1. 6 6. 0E-05 4885170 NT Homo sapiens chromosome X open reading frame 6 (CXORF6) mRNA 95 Page 62 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similor Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value _ wb54hO6. x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2309531 3'similar to gb : J03250 DNA 2513 7481 12597 1. 15 6. OE-05 Al655241. 1 EST HUMAN TOPOISOMERASE I (HUMAN) ; 27425696 10704 2. 86 6.0E-05 AF053630. 1 NT Homo sapiens monocyte/neutrophil elastase inhibitor gene, complete cds 1379 6376 11424 65.'; 5. 0E-05 AW392086. 1 EST HUMAN QV4-ST0234-241199-040-h11 ST0234 Homo sapiens cDNA 1826 6816 2. 39 5. OE-05 8923891 NT Homo sapiens 22kDa peroxisomal membrane protein-like (LOC55895), mRNA 2790 7811 12828 0. 675. 0E-05 AJ251058. 1 NT Homo sapiens MEP1A gene, promoter region and exon 1 3872 8873 13878 4. 04 5.0E-05 AJ251884. 1 NT Homo sapiens partial SLC22A3 gene for extraneuronal monoamine transporter (EMT), exon 1 2735 5289 3.87 4.0E-05 U12821. 1 NT Human renin (REN) gene, 5'flanking region 4355 9346 14325 0.92 4.0E-05 P49193 SWISSPROT RETINAL-BINDING PROTEIN (RALBP) 4355 9346 14326 0.92 4.0E-05 P49193 SWISSPROT RETINAL-BINDING PROTEIN (RALBP) 47259710 0. 99 4.0E-05 AF164488. 1 NT Cryptosporidium parvum isolate Zaire 15 kDa glycoprotein gp15 gene, partial cds qh64c10. x1 Soares_Fetal_liver_spleen_1NFLS_S1. Homo sapiens cDNA clone IMAGE : 1849458 3'similar to 672 5697 10706 0. 69 3.0E-05 AI248061. 1 EST HUMAN contains Alu repetitive element ; contains element KER repetitive element ; 1042 6052 11082 1. 01 3.0E-05 AW273851. 1 EST_HUMAN xv24g03.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2814100 3' 1114 6120 11148 1. 64 3.0E-05 BF037898. 1 EST_HUMAN 601461463F1 NIH_MGC_66 Homo sapiens cDNA done IMAGE : 3865142 5' 1114 6120 11149 1.64 3.0E-05 BF037898. 1 EST HUMAN 601461463F1 NIH MGC 66 Homo sapiens cDNA clone IMAGE : 3865142 5' 2648 7608 12720 0. 92 3.0E-05 Q6234 SWISSPROT SKELEMIN 4257 9251 14237 9. 42 3.0E-05 BE169211. 1 EST HUMAN PM1-HT0521-120200-001-e10 HT0521 Homo sapiens cDNA 4257 9251 14238 9. 42 3. OE-05 BE169211. 1 EST HUMAN PM1-HT0521-120200-001-e10 HT0521 Homo sapiens cDNA 4342 9333 14316 0. 94 3.0E-05 AA368679. 1 EST-HUMAN EST79996 Placenta l Homo sapiens cDNA similar to similar to p53-associated protein 4342 9333 14317 0.94 3.0E-05 AA368679.1 EST_HUMAN EST79996 Placenta I Homo sapiens cDNA similar to similar to p53-associated protein 4486 9476 14456 0.76 3.0E-05 AF149773.1 NT Homo sapiens NOD1 protein (NOD1) gene, exons 1, 2, and 3 4695 9680 14663 0.93 3.0E-05 Au125721. 1 EST HUMAN AU125721 NT2RM4 Homo sapiens cDNA clone NT2RM4002075 5' qh98e11. x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 1855052 3'similar to contains 2262 7239 12356 1.33 2.0E-05 AI286021. 1 EST HUMAN MER3. b2 MER3 repetitive element ; 2506 7474 12589 1. 85 2. OE-05 M13792. 1 NT Human adenosine deaminase (ADA) gene, complete cds zq46a12. r1 Stratagene hNT neuron (#937233) Homo sapiens cDNA clone IMAGE : 632734 5'similar to 2641 7601 5. 26 2.0E-05 AA160562. 1 EST HUMAN contains Alu repetitive element ; contains element L1 repetitive element ; 3064 8081 13095 1.66 2.0E-05 BE066036. 1 EST HUMAN 4 3272 8284 13307 0. 85 2. OE-05 AF184614. 1 NT Homo sapiens p47-phox (NCF1) gene, complete cds 3287 8298 13325 1. 06 2. OE-05 X89211. 1 NT H. sapiens DNA for endogenous retroviral like element 3414 8423 0.69 2.0E-05 X95465. 1 NT S. cerevisiae 12. 8 Kbp fragment of the left arm of chromosome XV 2623 7777 12694 1. 66 1. 0E-05 AL163282. 2 NT Homo sapiens chromosome 21 segment HS21 C082 35658572 13576 2. 01 1. 0E-05 AF088273. 1 NT Drosophila melanogaster strain Lamto 120 Suppressor of Hairless (Su (H)) gene, partial cds ! Page 63 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO : NO :... Source Value Homo sapiens calcium channel alpha subunit (CACNA1E) gene, exons 7-49, and partial cds, alternatively 3723 8727 1. 18 1. OE-05 AF223391. 1 NT spliced 3860 8862 13867 10. 08 1. OE-05 P81274 SWISSPROT MOSAIC PROTEIN LGN 4049 9045 14033 1. 04 1. OE-05 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 4155 9150 14132 1. 76 1. 0E-05 AA431119. 1 EST HUMAN zw69g04. r1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 781494 5' 4703 9688 14671 1. 9 1.0E-05 AW419134. 1 EST_HUMAN xy49g11. x1 NCl_CGAP_Lu34. 1 Homo sapiens cDNA clone IMAGE : 2856548 3' 2601 7563 12681 2. 89 9. OE-06 AI583811. 1 EST_HUMAN tt73a06.x1 NCI_CGAP_HSC3 Homo sapiens cDNA clone IMAGE : 2246386 3' 3022 8039 13048 4. 56 9. 0E-06 AI218983. 1 EST_HUMAN qg11b08.x1 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA clone IMAGE : 1759191 3' 3529 8535 2. 64 9. OE-06 M61755. 1 NT Human alanine : glyoxylate aminotransferase (AGXT) gene, exons 1 and 2 2458 7771 12543 1. 52 8. 0E-06 AW362539. 1 EST HUMAN RC3-CT0283-201199-011-h11 CT0283 Homo sapiens cDNA ab90f10. s1 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE : 854251 3'similar to contains 964 5979 1. 3 7.0E-06 AA669729. 1 EST HUMAN MER20. t1 MER20 repetitive element ; 1412 6410 11469 2. 42 7. 0E-06 7662177 NT Homo sapiens KIAA0555 gene product (KIAA0555), mRNA qw16g09.x1 NCI_CGAP_Ut3 Homo sapiens cDNA clone IMAGE : 1991296 3'similar to contains Alu repetitive 2802 7822 6. 96 7. 0E-06 AI368252. 1 EST HUMAN element ; 2847 7867 12883 1. 16 6. 0E-06 BE069189. 1 EST HUMAN QV3-BT0379-010300-105-d11 BT0379 Homo sapiens cDNA 2871 7890 12912 1. 03 6. 0E-06 Q01456 SWISSPROT OVARIAN ABUNDANT MESSAGE PROTEIN (OAM PROTEIN) 3609 8616 13625 0. 93 6. 0E-06 BE069189. 1 EST HUMAN QV3-BT0379-010300-105-d11 BT0379 Homo sapiens cDNA 4609 7890 12912 1. 96 6. OE-06 Q01456 SWISSPROT OVARIAN ABUNDANT MESSAGE PROTEIN (OAM PROTEIN) ox08e02. x1 SoaresfetaU'verspfeen1NFLSS1 Homo sapiens cDNA clone IMAGE : 1655738 3'similar to 4616 9601 14587 2. 14 6. 0E-06 A1040099. 1 EST HUMAN contains MER8. t2 MER8 repetitive element ; ya48c03. r1 Soares infant brain 1 NIB Homo sapiens cDNA clone IMAGE : 53254 5'similar to contains Alu 639 5667 10670 5. 76 4. 0E-06 R16267. 1 EST HUMAN repetitive element ; contains L1 repetitive element ; xc69g12.x1 NCI_CGAP_Eso2 Homo sapiens cDNA clone IMAGE : 2589574 3'similar to contains Alu 836 5855 10895 6. 98 4. OE-06 AW 103354. 1 ESTHUMAN repetitive etement : contains efement MER21 repetitive element ; 1314 6311 11359 3. 35 4. OE-06 AI334928. 1 EST_HUMAN tb33e09.x1 NCI_CGAP_HSC2 Homo sapiens cDNA clone IMAGE : 2056168 3' 1314 6311 11360 3. 35 4. OE-06 At334928. 1 EST_HUMAN tb33e09.x1 NCI_CGAP_HSC2 Homo sapiens cDNA clone IMAGE : 2056168 3' 1445 6442 11500 1. 85 4. OE-06 BF365612. 1 EST HUMAN QV2-NT0046-200600-250-hO7 NT0046 Homo sapiens cDNA 2203 7181 12304 1. 59 4. OE-06 AW015401. 1 EST HUMAN Ul-H-BlO-aat-f-05-0-Ul. s1 NCI_CGAP Sub1 Homo sapiens cDNA clone IMAGE : 2710425 3' 2990 8008 13021 0. 74 4. OE-06 AF198349. 1 NT Gallus gallus Dach2 protein (Dach2) mRNA, complete cds 3799 8802 13807 1. 07 4. 0E-06 AW848295. 1 EST_HUMAN IL3-CT0214-150200-074-B03 CT0214 Homo sapiens cDNA wl94c10. x1 NCl_CGAP_Brn25 Homo sapiens cDNA clone MAGE : 2432562 3'similar to contains element- 4663 9648 14635 1. 95 4. OE-06 AI886939. 1 EST HUMAN MER22 repetitive element ; Page 64 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Q..-MostSimiiar-,,., Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Eression (Top) Hit Top Hit Acession Top Hit Descriptor ID NO : Signal BLAST E No. Database NO : NO : Value Source, 5047 10018 14987 1. 04 4. OE-06 AW817268. 1 EST HUMAN QVO-ST0247-090200-105-c05 ST0247 Homo sapiens cDNA 34b08. s1 Soares fetal liver spleen_1 NFLS S1 Homo sapiens cDNA clone IMAGE : 432663 3'similar to 2099 7080 12194 1. 29 3. OE-06 AA700562. 1 EST HUMAN contains Ll. t1 L1 repe66ve element ; zi34b08. s1 Soares fetal liver spleen 1 NFLS S1 Homo sapiens cDNA clone IMAGE : 432663 3'similar to 2099 7080 12195 1. 29 3. OE-06 AA700562. 1 EST HUMAN contains L1. t1 L1 repetitive element ; 2205 7182 1. 19 3. OE-06 AF202635. 1 NT Homo sapiens PP1200 mRNA, complete cds ak48g11. s1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 1409252 3'similar to contains LTR1. t3 2851 7871 12886 1. 09 3. OE-06 AA868218. 1 EST HUMAN LTR1 repeti6ve element ; w122a05. x1 NCI_CGAP_Ut1 Homo sapiens cDNA clona IMAGE : 2425616 3'similar to TR : 060734 060734 3191 8207 2. 25 3. OE-06 AI857779. 1 EST HUMAN LINE-1 LIKE PROTEIN ; contains L1. t2 L1 repeti6ve element ; 3695 8699 13701 1. 73 3. OE-06 BE047094. 1 EST HUMAN hq64d12. x1 NCI_CGAP I-IN13 Homo sapiens cDNA clone IMAGE : 3124151 3' 3695 8699 13702 1. 73 3. OE-06 BE047094. 1 EST HUMAN hq64d12. x1 NCI CGAP HN13 Homo sapiens cDNA clone IMAGE : 3124151 3' Homo sapiens gene for alpha-1-microglobulin-bikunin, econs 1-5 (encoding alpha-1-microglobulin, N- 4430 9420 14406 2. 51 3. OE-06 X54816. 9 NT terminus.) 4766 9750 14738 0. 95 3. OE-06 J04038. 1 NT Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, complete cds 4766 9750 14739 0. 95 3. OE-06 J04038. 1 NT Human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene, complete cds 202 5266 2. 36 2. OE-06 P54366 SW ISSPROT HOMEOBOX PROTEIN GOOSECOID 1535 6533 4. 34 2. OE-O6 P21414 SW ISSPROT POL POLYPROTEIN [GONTAINS : PROTEASE ; REVERSE TRANSCRIPTASE ; ENDONUCLEASE] wa04a03. x1 NCI_CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2297068 3'similar to contains MER30. b1 2315 7290 12411 3. 21 2. OE-06 AI672138. 1 EST HUMAN MER30 repe66ve element ; 2397 7368 12489 1. 48 2. OE-06 P04929 SWISSPROT HISTIDINE-RICH GLYCOPROTEIN PRECURSOR 2494 7462 12577 4. 19 2. OE-06 P06719 SW SSPROT KNOB-ASSOCIATED HISTIDINE-RICH PROTEIN PRECURSOR (KAHRP) 3441 8449 13475 1. 07 2. OE-06 AV657555. 1 EST_HUMAN AV657555 GLC Homo sapiens cDNA clone GLCFDB05 3' 3676 8681 13684 1. 7 2. OE-06 AA173518. 1 EST HUMAN zp02e05. r1 Stratagene ovarian cancer (#1937219) Homo sapiens cDNA clone IMAGE : 595232 5' 3689 8693 13695 1. 8 2. OE-06 AB030896. 1 NT Mus musculus gene for odorant receptor A16, complete cds ORGANIC CATION/CARNITINE TRANSPORTER 2 (SOLUTE CARRIER FAMILY 22, MEMBER 5) (HIGH -.'' 34 5114 10100 1. 11 1. OE-06 076082 SWfSSPROT AFFINITYSODIUM-DEPENDENT CARNITINE COTRANSPORTER) 649 5677 10684 1. 24 1. OE-06 AF084364. 1 NT Mus musculus D6MM5E protein (D6Mm5e) mRNA, complete cds, 1425 6422 11481 1. 53 1. OE-06 P09125 SW ISSPROT MEROZOITE SURFACE PROTEIN CMZ-8 1491 6489 11544 0. 92 1. OE-06 AL163278. 2 NT Homo sapiens chromosome 21 segment HS21 C078 1948 6934 12034 3. 32 1. OE-06 AF184614. 1 NT Homo sapiens p47-phox (NCF1) gene, complete cds 1948 6934 12035 3. 32 1. OE-06 AF184614. 1 NT Homo sapiens p47-phox (NCF1) gene, complete cds 4244 9238 14221 13. 65 1. OE-06 U07561. 1 NT Human ABL gene, exon 1 b and intron 1 b, and putative M8604 Mef protein (M86Q4 Met) gene, complete cds :' a Page 65 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis -...-MostSimiiar-,,.. Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Eression (Top) Hit Top Hit Acession Top Hit Descriptor ID NO : Signal BLAST E No. Database NO : NO : Source, Value 4933 9910 14887 1. 24 1. OE-O6 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 4933 9910 14888 1. 24 1. OE-06 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 359 5409 10421 1. 02 9. OE-07 AF003529. 1 NT Homo sapiens glypican 3 (GPC3) gene, partial cds and flanking repeat regions 359 5409 10422 1. 02 9. OE-07 AF003529. 1 NT Homo sapiens glypican 3 (GPC3) gene, partiai cds and flanking repeat regions 4625 9610 14598 5. 07 8. OE-07 AI28896. 1 EST HUMAN q182g07. x1 Soares NhHMPu S1 Hano sapiens cDNA clone IMAGE : 1878876 3' 4625 9610 14599 5. 07 8. OE-07 AI288596. 1 EST HUMAN q182g07. x1 Soares NhHMPu S1 Homo sapiens cDNA clone IMAGE : 1878876 3' 1868 6857 11945 2. 73 6. OE-07 AW 855558. 1 EST HUMAN CM3-CT0277-221099-024-e11 CT0277 Homo sapiens cDNA Homo sapiens HLA class III region containing tenascin X (tenascin-X) gene, partial cds ; cytochrome P450 21- hydroxylase (CYP21 B), complement component C4 (C4B) G11, helicase (SKI2W), RD, complement factor B 2429 7392 12513 2. 45 6. OE-07 AF019413. 1 NT (B, and complement component C2 (C2) genes, > 3865 8867 1. 99 6. OE-07 P41479 SW ISSPROT HYPOTHETICAL 24. 1 KD PROTEIN IN LEF4-P33 INTERGENIC F2EGION 324 5378 0. 9 5. OE-07 AI831893. 1 EST HUMAN wh64f10. x1 NCI CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2385547 3' 1040 6050 1. 83 5. OE-07 AA380630. 1 EST HUMAN EST93615 Supt cells Homo sapiens cDNA 5'end 4511 9501 14480 1. 28 S. OE-07 AF149774. 1 NT Homo sapiens NOD1 protein (NOD1) gene, exons 4 through 14 and complete cds 3893 8893 13892 1. 86 4. OE-07 AW 009602. 1 EST HUMAN ws84h05. x1 NCI_CGAP Co3 Homo sapiens cDNA clone IMAGE : 2504697 3' Human microfibril-associated glycoprotein (MFAP2) gene, puta6ve promoter region and alternatively spliced 438 5476 90492 5. 45 3. OE-07 U19719. 1 NT untransfated exons 578 5610 10609 2. 79 3. OE-07 AJ271735. 1 NT Homo sapiens Xq pseudoaufosomai region ; segment 9 ! 2 1356 6353 11403 1. 32 3. OE-07 M99149. 1 NT Human polymaphic microsatellite DNA 1586 6583 1. 79 3. OE-07 M64857. 1 NT Human IgK subgroup I germline gene, exons 1 and 2, V-region 018 allele 2224 7201 12320 0. 94 3. OE-07 M99149. 1 NT Human polymorphic microsatellite DNA. 2399 7370 12491 26. 28 3. OE-07 BE005077. 1 EST HUMAN MRO-BN0115-020300-001-f11 BN0115 Homo sapiens cDNA 2399 7370 12492 26. 28 3. OE-07 BE005077. 1 EST HUMAN MRO-BN0115-020300-001-f11 BN0115 Homo sapiens cDNA 2964 7982 12996 0. 85 3. OE-07 T84704. 1 EST HUMAN yd50f12. r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 111695 5' 3086 8102 13117 1. 64 3. OE-07 P38739 SW ISSPROT HYPOTHET (CAL 63. 8 KD PROTEIN IN GUT1-RIM1 INTERGENIC REGION PRECURSOR 4587 9575 14566 8. 15 3. OE-07 AV650201. 1 EST HUMAN AV650201 GLC Homo sapiens cDNA clone GLCCCD01 3'. yc14h09. s1 Stratagene lug (#1937210) Homo sapiens cDNA clone IMAGE : 80705 3'similar to similar to 4895 9874 14841 1. 6 3. OE-07 T57850. 1 EST HUMAN gb : M62982 ARACHIDONATE 12-LIPOXYGENASE (HUMAN) yc14h09. s1 Sfratagene lung (#t937210) Homo sapiens cDNA clone IMAGE : 80705 3'similar to similar to 4895 9874 14842 1. 6 3. OE-07 T57850. 1 EST HUMAN gb : M62982 ARACHIDONATE 12-LIPOXYGENASE (HUMAN) 29 5109 10094 2. 94 2. OE-07 AF262988. 1 NT Homo sapiens TRF2-interacting telomeric RAP1 protein (RAP1) mRNA, complete cds 153 5219 10231 23. 84 2. OE-07 L77569. 1 NT Homo sapiens DiGeorge syrtdrome cri6cal region, telomeric end 153 5219 10232 23. 84 2. OE-07 L77569. 1 NT Homo sapiens DiGeorge syndrome critical region, telomeric end 181 5244 10255 129. 68 2. OE-07 U38849. 1 NT Fugu rubripes beta-cytoplasmic (vascula) actin gene, complete cds Page 66 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Eression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Database NO : NO : Value Sce, 739 5762 10785 1. 46 2. OE-07 AF003530. 1 NT Homo sapiens homeobox protein CDX4 (CDX4) gene, complete cds and flanking repeat regions 739 5762 10786 1. 46 2. OE-07 AF003530. 1 NT Homo sapiens homeobox protein CDX4 (CDX4) gene, complete cds and flanking repeat regions u08b07. s1 Stratagene NT2 neuronal precursor 937230 Homo sapiens cDNA clone IMAGE : 650869 3'similar 929 5946 10980 1. 99 2. OE-07 AA223260. 1 EST HUMAN to gb : L31860 GLYCOPHORIN A PRECURSOR (HUMAN) ; contains Alu repetitive element ; yc15g04. s1 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE : 80790 3'similar to contains L1 930 5947 10981 11. 68 2. OE-07 T63042. 1 EST HUMAN repe66ve element ; 1145 6149 11181 0. 93 2. OE-07 Q26768 SW ISSPROT l6 AUTOANTIGEN 1565 6562 11625 2. 13 2. OE-07 Q09701 SW ISSPROT HYPOTHETICAL 72. 5 KD PROTEIN C2F7. 10 IN CHROMOSOME I 3604 8611 13619 20. 05 2. OE-07 AF125348. 1 NT Homo sapiens caveolin 1 (CAV1) gene, exon 3 and partial cds 1085 6092 1. 43 1. OE-07 AL163282. 2 NT Homo sapiens chromosome 21 segment HS21 C082 2754 6488 11543 2. 14 1. OE-07 P09256 SWISSPROT GLYCOPROTEIN GPV 3659 6092 3. 91 1. OE-07 AL163282. 2 NT Homo sapiens chromosome 21 segment HS21 C082 4169 9164 14150 2. 86 1. OE-07 AV718662. 1 EST HUAAAN AV718662 GLC Homo sapiens cDNA clone GLCFNF04 5' 4169 9164 14151 2. 86 1. OE-07 AV718662. 1 EST HUMAN AV718662 GLC Homo sapiens cDNA clone GLCFNF04 5' 4973 9949 14927 1. 22 1. OE-07 AA019181. 1 EST HUMAN ze56g02. r1 Soares etina N2b4HR Homo sapiens cDNA clone IMAGE : 363026 5' 604 7725 2. 23 8. OE-08 AI911352. 1 EST HUMAN wd16b05. x1 Soares NFL_T GBC S1 Homo sapiens cDNA clone IMAGE : 2328273 3' 1033 6043 0. 8 8. OE-08 BE795469. 1 EST HUMAN 601590133F1 NIH MGC 7 Homo sapiens cDNA clone IMAGE : 3943976 5' 3468 8476 1. 7 8. OE-08 BE795469. 1 EST HUMAN 601590133F1 NIH_MGC 7 Homo sapiens cDNA clone IMAGE : 3943976 5' 79 5156 10167 3. 2 7. OE-08 Q02357 SW ISSPROT ANK1RIN 1 (ERYTHROCYTE ANKYRIN) : 1344 6341 11392 42. 98 7. OE-08 X04809. 1. NT Rat mRNA for ribosomal protein L31 3495 8503 13517 0. 7 7. OE-08 P15305 SW ISSPROT DYNEIN HEAVY CHAIN (DYHC) 3495 8503 13518 0. 7 7. OE-08 P15305 SW ISSPROT DYNEIN HEAVY CHAIN 807 5828 10858 2. 78 6. OE-08 AL163248. 2 NT Homo sapiens chromosome 21 segment HS21 C048 807 5828 10859 2. 78 6. OE-08 AL163248. 2 NT Homo sapiens chromosome 21 segment HS21 C048- 2302 7277 12397 3. 8 6. OE-08 BE144398. 1 EST HUMAN MRO-HT0166-191199-004-g09 HT0166 Homo sapiens cDNA 2g88 8006 13019 2. 09 6. OE-08 7662473 NT Homo sapiens KIAA1074 protein (KIAA1074), mRNA 4126 9121 14106 0. 92 6. OE-08 AL163248. 2 NT Homo sapiens chromosome 21 segment HS21 C048 83 5160 10171 2. 15 5. OE-08 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 nh03b09. s1 NCI_CGAP Thyl Homo sapiens cDNA clone IMAGE : 943193 simitar to contains Alu repetitive 2174 7153 12273 1. 68 5. OE-08 AA493851. 1 EST HUMAN element ; 1722 6717 11793 1. 2 4. OE-08 P25723 SW ISSPROT DORSAL-VENTRAL PATTERNING TOLLOID PROTEIN PRECURSOR 1722 6717 11794 1. 2 4. OE-08 P25723 SW ISSPROT DORSAL-VENTRAL PATTERNING TOLLOID PROTEIN PRECURSOR 2815 7835 1. 1 4. OE-08 AL079581. 1 EST HUMAN DKFZp434J0426 r1 434 (synonym : htes3) Homo sapiens cDNA clona DKFZp434J0426 5'_' : Page 67 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQID SEQID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor NO : NO : ID NO : Signal BLAST E No. Source Valut 206 5270 8. 87 2. OE-08 AW302996. 1 EST_HUMAN xr87f06.x1 NCI_CGAP_Lu26 Homo sapiens cDNA clone IMAGE : 2767139 3' zw48f07. r1 SoarestotatfetusNb2HF89w Homo sapiens cDNA clone IMAGE : 773317 5'similar to contains 227 5290 7. 99 2. 0E-08 AA425598. 1 EST HUMAN Alu repeti6ve etement ; contains element MER15 repetitive element ; 493 5529 10537 2. 63 2. OE-08 AF198349. 1 NT Gallus gallus Dach2 protein (Dach2) mRNA, complete cds 652 5680 10686 9. 13 2. OE-08 AW886438. 1 EST HUMAN MRO-OT0080-240200-001-g08 OT0080 Homo sapiens cDNA 652 5680 10687 9. 13 2. OE-08 AW886438. 1 EST HUMAN MRO-OT0080-240200-001-g08 OT0080 Homo sapiens cDNA 975 5991 26. 56 2. 0E-08 BE280477. 1 EST HUMAN 601155321F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3138893 5' 1323 6321 11369 1. 74 2. 0E-08 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21C047 1702 6697 1. 75 2. OE-08 BE734871. 1 EST_HUMAN 601570463F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3845199 5' 1818 6808 3. 33 2. 0E-08 AW270271. 1 EST HUMAN xp43f11. x1 NCI CGAP HN11 Homo sapiens cDNA clone IMAGE : 2743149 3' nw64h01. s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE : 1251409 3'similar to contains L1. t3 L1 2352 7326 12443 1. 22 2. 0E-08 AA731948. 1 EST HUMAN repetitive element ; 2472 7441 1. 31 2. OE-08 K00216. 1 NT Sheep His-tRNA-GUG 3135 8151 13173 6. 15 2. OE-08 042280 SWISSPROT WNT-14 PROTEIN PRECURSOR 3135 8151 13174 6. 15 2. 0E-08 042280 SWISSPROT WNT-14 PROTEIN PRECURSOR 3769 8772 1. 61 2.0E-08 AW813620. 1 ESTHUMAN RC3-ST0197-161099-012-b03 ST0197 Homo sapiens cDNA aa26c07. r1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE : 814380 5'similar to contains L1. t2 L1 4281 9274 1. 32 2. 0E-08 AA459040. 1 EST HUMAN repetitive element ; he17h08. x2 NC) CGAPCML1 Homo sapiens cDNA clone IMAGE : 2919327 3'similar to contains Alu 4800 9784 3. 44 2.0E-08 AW572881.1 EST_HUMAN repetitive element ; 1738 6733 11810 1. 26 1. OE-08 AF125348. 1 NT Homo sapiens caveolin 1 (CAV1) gene, exon 3 and partial cds 1998 6981 1. 58 1. 0E-08 BE141959.1 EST_HUMAN PM2-HT0130-150999-001-f12 HT0130 Homo sapiens cDNA TCBAP1D5232 Pediatric pre-B cell acute Iymphoblastic leukemia Baytor-HGSC projet=TCBA Homo 3119 8135 13155 1. 06 1. OE-08 BE246844. 1 EST HUMAN sapiens cDNA clone TCBAP5232 TCBAP1D5232 Pediatric pre-B cell acute lymphoblastic leukemia Baylor-HGSC project=TCBA Homo 3119 8135 13156 1. 06 1. OE-08 BE246844. 1 EST HUMAN sapiens cDNA clone TCBAP5232 4120 9114 14100 3. 28 9. OE-09 AL163279. 2 NT Homo sapiens chromosome 21 segment HS21C079 4120 9114 14101 3. 28 9. 0E-09 AL163279. 2 NT Homo sapiens chromosome 21 segment HS21 C079 3526 8532 1. 62 7. 0E-09 D86842. 1 NT Homo sapiens DNA for 3-ketoccyl-CoA thiolase beta-subunit of mitochondrial trifunctional protein, exon 2, 3 4310 9302 14287 1. 19 6. 0E-09 AF111167. 2 NT Homo sapiens jun dimerization protein gene, partial cds ; cfos gene, complete cds; and unknown gene 4821 9805 14787 4. 99 6. 0E-09 BE169421. 1 EST HUMAN PM1-HT0527-160200-001-h05 HT0527 Homo sapiens cDNA 1390 6387 11439 2. 89 5. 0E-09 BE149264. 1 EST_HUMAN RC2-HT0252-120200-014-h10 HT0252 Homo sapiens cDNA Page 68 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession pabase Top Hit Descriptor ID NO : Signal BLAST E Nw Source NO : NO : Value 1816 6806 11899 1. 29 5. OE-09 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 517 5552 1. 41 4. OE-09 AL163282. 2 NT Homo sapiens chromosome 21 segment HS21C082 950 5966 2. 28 4. OE-09 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 1441 6438 11495 2. 05 4. OE-09 9558718 NT Homo sapiens hypothetical potein (AF038169), mRNA 2362 7336 12453 17. 61 4. OE-09 AA350878. 1 EST HUMAN EST58385 Infant brain Homo sapiens cDNA 5'end similar to similar to heat shock protein, 90 kDa hu09e09. x1 NCI_CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3166120 3'similar to contains MER18. t3 2290 7265 12383 3. 39 3. OE-09 BE222239, 1 EST HUMAN MER18 repeti6ve element ; hu09e09. x1 NCI_CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3166120 3'similar to contains MER18. Y3 24g2 7450 12563 1. 04 3. OE-09 BE222239. 1 EST HUMAN MER18 repetitive element ; 2578 7541 12655 0. 92 3. 0E-09 P23249 SW) SSPROT PROTEIN MOV-10 hu09e09. x1 NCI_CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3166120 3'similar to contains MER18. 13 2578 7541 12655 0. 92 3. OE-09 P23249 SW ISSPROT PROTEIN MOV-10 3254 8267 13289 1. 29 3. OE-09 BE222239. 1 EST_HUMAN MER18 repetitive element ; 32g2 8303 5. 3 3. OE-09 AA442272. 1 EST HUMAN zv54a04. r1 Soares testis_NHT Homo sapiens cDNA clone IMAGE : 757422 5' 4302 9294 14281 4. 42 3. OE-09 AF175325. 1 NT Hamo sapiens eukaryotic ini6ation factor 4AI (EIF4A1) gene, partial cds 4381 9372 14351 1. 63 3. OE-09 Q9Y3R5 SW ISSPROT 258. 1 KDA PROTEIN C210RF5 (KIAA0933) 1239 6237 11280 13. 77 2. OE-09 AL163284. 2 NT Homo sapiens chomosome 21 segment HS21 C084 1618 6615 14. 91 2. OE-09 AL118573. 1 EST HUMAN DKFZp761B1710 r1 761 (synonym : hamyl) Homo sapiens cDNA clone DKFZp761B1710 5' 2265 7242 12359 8. 68 2. oE-09 Q9Y3R5 SW ISSPROT 258. 1 KDA PROTEIN C21 ORF5 (KIAA0933) 3834 8s36 13842 3. 1 2. OE-09 060241 SW ISSPROT BRAN-SPECIFIC ANGIOGENESIS INHIBITOR 2 PRECURSOR 1092 6099 11128 1. 68 1. OE-09 5031624 NT Homo sapiens CCAAT-box binding transcription factor (CBF2) mRNA 6099 11129 1. 68 1. OE-09 5031624 NT Homo sapiens CCAAT-box-binding Uanscription factor (CBF2) mRNA 1092 qy64e11. x1 NCI_CGAP Bn25 Homo sapiens cDNA clone IMAGE : 2016812 3'similar to contains MER12. t2 2428 7399 1. 35 1. OE-09 AI356086. 1 EST HUMAN MER12 repeti6ve element ; Homo sapiens basic transcrip6on factor 2 p44 (btf2P4) 9ene, partial cds, neuronai apoptosis inhibitory 7839 12856 1. 69 1. OE-09 U80017. 1 NT protein (naip) and survival motor neuon protein (smn) genes, complete cds 2ggp 17. 33 1. OE-09 M28699. 1 NT Homo sapiens nucleolar phosphoprotein B23 (NPM1) mRNA, complete cds 2819 2855 7875 12891 17. 33 1. oE-09 M28699. 1 NT Homo sapiens nucleolar phosphoprotein B23 (NPM1) mRNA, complete cds 2855 7875 2966 7984 12998 0. 69 1. OE-09 BE535440. 1 EST HUMAN 601058602F1 NIH MGC 10 Homo sapiens cDNA clone IMAGE : 3445177 5' zh35b03. s1 Soaresinealland_N3HPG Homo sapiens cDNA clone IMAGE : 414029 3'similar to contains _ 9639 6. 25 1. OE-09 AA7192971 EST HUMAN Alu repetitive element ; contains element MER22 repe6tiva element ; 1290 6289 11333 1. 94 9. OE-10 AW 867740. 1 EST HUMAN MRO-SN0040-050500-002-c07 SN0040 Homo sapiens cDNA 4654 we78h03. x1 Soares_Dieckgraefe_colon_NHCD Homo sapiens cDNA clone IMAGE : 2347253 3'similar to 914 60S RiBOSOMAL PROTEIN L29 ; contains element PTR5 repetitive element ; 2760 7781 12806 7. 26 9. OE-10 AI870071. 1 EST HUMAN SW : RL29 HUMAN P47 Page 69 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession pabasa Top Hit Descriptor ID NO : Signal BLAST E N Souce NO : NO : Value 146 5212 10226 11. 08 8. OE-10 U63630. 2 NT Homo sapiens MCM4 (MCM4) and DNA-PKcs (PRKDC) genes, partial cds 4078 9072 14059 1. 87 8. OE-10 AA36832. 1 EST HUMAN EST89564 Small intestine I Homo sapiens cDNA 5'end 692 5716 10732 39. 41 7. OE-10 7706225 NT Homo sapiens TPA inducible protein (LOC51586), mRNA 692 5716 10733 39. 41 7. OE-10 7706225 NT Homo sapiens TPA inducible protein (LOC51586), mRNA 1583 6580 11643 1. 69 7. OE-10 Q13342 SW ISSPROT LYSP100 PROTEIN (LYMPHOID-RESTRICTED HOMOLOG OF SP100) 2488 7456 9. 17 7. OE-10 P08547 SW ISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 3014 8031 13041 2. 59 7. OE-10 X00$56. 1 NT H. sapiens DHFR gene exon 3 Homo sapiens ASCL3 gene, CEGP1 gene, C11orf14 gene, C11orf15 gene, C11orf16 gene and C11orf17 902 5920 10956 2. 89 6. OE-10 AJ400877. 1 NT gene 2606 7568 12683 1. 11 6. OE-10 AI424405. 1 EST HUMAN tf2d07. x1 NCI_CGAP Pr28 Homo sapiens cDNA clone IMAGE : 2095021 3' 4598 9586 2. 52 6. OE-10 AW 853719. 1 EST HUMAN RC3-CT0254-031099-012-g12 CT0254 Homo sapiens cDNA 751 5773 4. 91 5. OE-10 AL046804. 1 EST NUMAN DKFZp434N219 r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434N219 5' 3395 8403 13429 1. 15 5. OE-90 Q01033 SW ISSPROT HYPOTHETICAL GENE 48 PROTEIN 4833 9817 14795 1. 25 5. OE-10 AF181897. 1 NT Homo sapiens W RN (W RN) gene, complete cds qg09f09. x1 Soareslacenta 8to9weeks 2NbHP8to9W Homo sapiens cDNA clone IMAGE : 1759049 3' 111 5183 1. 48 4. OE-10 AI221083. 1 EST HUMAN similar to contains LTR8. b2 LTR8 repetitive element ; 576 5608 10607 1. 35 4. OE-10 AA515260. 1 EST HUMAN nf64a01. s1 NCI_CGAP Co3 Homo sapiens cDNA clone IMAGE : 924648 3' hg58g03. x1 NCI_CGAP GC6 Homo sapiens cDNA clone IMAGE : 2949844 3'similar to contains Alu 1950 6936 12037 1. 75 4. OE-10 AW 594709. 1 EST HUMAN repeti8ve element ; 2499 7467 12582 3. 14 4. OE-10 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 yy32f06. s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 272963 3'similar to contains 904 5921 10958 1. 72 3. OE-10 N36113. 1 EST HUMAN L1. t1 L1 repe6tive element ; 6331 4. 3 3. OE-10 AY005150. 1 NT Homo sapiens extracellular glycoProtein lacritin precursor, gene, complete cds 1333 CENP B 5116 10102 1. 49 2. OE-10 P48988 SW ISSPROT MAJOR CENTROMERE AUTOANTIGEN B (CENTROMERE PROTEIN B) (-) 116 90903 1. 49 2. OE-10 P48988 SW ISSPROT MAJOR CENTROMERE AUTOANTIGEN B (CENTROMERE PROTEIN B) (CENP-B) 36 36 5 Homo sapiens basic transcrip6on factor 2 p44 (btf2p44) gene, partial cds, neuronal apoptosis inhibitory 1857 6s46 2. 98 2. OE-10 U80017. 1 NT protein (naip) and survival motor neuron protein (smn) genes, complete cds 1. 37 2. OE-10 BF675047. 1 EST HUMAN 602136640F1 NIH MGC 83 Homo sapiens cDNA clone IMAGE : 4273377 5' 1479 6476 1. 01 1. OE-10 AW 867767. 1 EST HUMAN MRO-SN0038-290300-001-f01 SN0038 Homo sapiens cDNA 1570 6567 11629 2. 18 1. OE-10 AV652123. 1 EST HUMAN AV652123 GLC Homo sapiens cDNA clone GLCCXA11 3' 7473 2. 43 1. OE-10 AW 852001. 1 EST HUMAN QVO-CT0225-191199-058-e08 CT0225 Homo sapiens cDNA p 8428 13454 0. 69 1. OE-10 AW832992. 1 EST HUMAN QV2-TT0003-161199-013-g10 TT0003 Homo sapiens cDNA 2505 8470 0. 97 1. OE-10 AL041685. 1 EST HUMAN DKFZp434N1317 r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434N1317 5', 342 470 1. 16 1. OE-10 AL041685. 1 EST HUMAN DKFZp434N1317 r1 434 (synonm : htes3) Homo sapiens cDNA clone DKFZp434N1317 5' 3462 3754 8, Page 70 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession ! D NO : Signal BLAST E No, Database Sourca NO : NO : Value Homo sapiens nuclear factor of kappa light pdypep6de gene enhancer in B-celis 1 (NFKB1) gene, complete 3908 8908 6. 93 1. OE-10 AF213884. 1 NT cds Homo sapiens X28 region near ALD locus containing dual speccity phosphatase 9 (DUSP9), ribosomal protein LlBa (RPL18a), Ca2+ICalmodulin-dependent protein kinase I (CAMKI), creatine transporte (CRTR), 4007 9003 13990 4. 83 1. oE-10 U52111. 2 NT CDM protein (CDM), adrenoleukodystrophY protein > Homo sapiens X28 region near ALD locus containing dual specificity phosphatase 9 (DUSP9), ribosomal protein L18a (RPL18a), Ca2+ICalmodulin-dependent protein kinase I (CAMKI), creatine transporter (CRTR), 4007 9003 13991 4. 83 1. OE-10 U52111. 2 NT CDM protein (CDM), adrenoleukodystrophy protein > 4013 9009 13999 2. 16 1. OE-10 A8031069. 1 NT Homo sapiens PCCX1 mRNA for protein containing CXXC domain 1, complete cds 4046 9042 2. 35 1. OE-10 M30629. 1 NT Human pregnancy-specific glycoprotein beta-1 (SP1) mRNA, last exon 259 5318 10328 1. 16 9. OE-11 BE145600. 1 EST_HUMAN IL2-HT0203-29109&016-c08 HT0203 Homo sapiens cDNA 2046 7028 12139 4. 32 9. OE-11 AL134395. 1 EST I-IUMAN DKFZp547D225_r1 547 (synonym : hfbr1) Homo sapiens cDNA clone DKFZp547D225 5' 2046 7028 12140 4. 32 9. OE-11 AL134395. 1 EST HUMAN DKFZp547D225 r1 547 (synonym : hfbr1) Homo sapiens cDNA clone DKFZp547D225 5' 32gg 8310 13337 2. 54 9. OE-11 AL134395. 1 EST hiUMAN DKFZp547D225 r1 547 (synonym : hfbrl) Homo sapiens cDNA clone DKFZp547D225 5' 3299 8310 13338 2. 54 9. OE-11 AL134395. 1 EST HUMAN DKFZp547D225 r1 547 (synonyn'i : hfbr1) Homo sapiens cDNA clone DKFZp547D225 5' 4371 9363. 14343 0. 69 9. OE-11 AA775985. 1 EST HUMAN ae78f01. s1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE : 970297 3' yn53f11. s1 Soares adult brain N2b5HB55Y Homo sapiens cDNA clone 1MAGE : 172173 3'similar to contains 3042 8059 8. 11 8. oE-11 H19971. 1 EST HUMAN L1 repetitive element ;- 3930 8930 13921 4. 2 8. OE-11 N23712. 1 EST HUMAN YW46e06. s1 W eizmann Olfactory Epithelium Homo sapiens cDNA clone IMAGE : 255298 3' 1422 6419 11479 1. 51 7. OE-11 AA330642. 1 EST HUMAN EST34392 Embryo, 6 week i Homo sapiens cDNA 5'end 409 5446 10467 5. 12 6. OE-11 M55270. 1 NT Human matrix Gla protein (MGP) gene, complete cds 409 5446 10468 5. 12 6. OE-11 M55270. 1 NT Human matrix Gla protein (MGP) gene, complete cds 12 5092 10076 0. 93 5. OE-11 AL163283. 2 NT Homo sapiens chromosome 21 segment HS21 C083 3284 5092 10076 0. 92 5. OE-11 AL163283. 2 NT Homo sapiens chromosome 21 segment HS21 C083 4107 9101 14088 1. 53 5. OE-11 P48034 SW ISSPROT ALDEHYDE OXIDASE 6374 5. 75 4. OE-11 AA436042. 1 EST HUMAN zu1b12. r1 Soares testis NHT Homo sapiens cDNA clone 7675 1278s 4. 93 4. oE-19 BE885900. 1 EST HUMAN 601507531F1 NIH MGC 71 Homo sapiens cDNA clone IMAGE : 3909295 5' 1377 2900 7919 12940 1. 26 4. OE-11 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21 C047 g5 g475 14455 0. 75 4. OE-11 D44666. 1 EST HUMAN HUMSUPY069 Human brain cDNA Homo sapiens cDNA clone 069 1460 6457 11516 18. 61 3. OE-11 6679077 NT Mus musculus expresser ! in non-metastatic cells 2, protein (NM23B) (Nme2), mRNA p, g 3. OE-11 AI816933. 1 EST HUMAN W1306. x1 NCI_CGAP Kid12 Homo sapiens cDNA clone IMAGE : 2404811 3' 4154 9149 1. 05 3. OE-11 AA309248. 1 EST HUMAN EST180120 Liver, hepatocellular carcinoma Homo sapiens cDNA 5'end qf36c04. x1 Soares tes6s_NHT Homo sapiens cDNA clone IMAGE : 1752102 3'similar to contains MER10. 945 5962 10995 1. 02 2. OE-11 AI150502. 1 EST HUMAN MER10 repeBfiva element ; j Page 71 of 209 Table 4 Sinble Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Value 1166 6169 11204 3. 84 2. 0E-11 R24807. 1 EST HUMAN yg43e12. r1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 35144 5' 1166 6169 11205 3.84 2. 0E-11 R24807.1 EST_HUMAN yg432e12.r1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 35144 5' Gallus gallus rho-globin, beta-H globin, beta-A globin, epsilon-globin, and olfactory receptor-like protein 1576 6573 11634 3. 97 2. 0E-11 L17432.1 NT COR3'beta (COR3'beta) genes, complete cds Gallus gallus rho-globin, beta-H globin, beta-A globin, epsilon-globin, and olfactory receptor-like protein 1576 6573 11635 3. 97 2. 0E-11 L1 7432. 1 NT COR3'beta (COR3'beta) genes, complete cds 2691 7649 12763 0. 93 2. 0E-11 AF087913. 1 NT Human endogenous retrovirus HERV-P-T47D 3123 8139 13161 5. 68 2. OE-11 P10263 SWISSPROT RETROVIRUS-RELATED GAG POLYPROTEIN (VERSION 1) 3251 8264 13285 0. 74 2. 0E-11 AI478617. 1 EST HUMAN tm54cO9. x1 NCI CGAP Kid11 HomosapienscDNAclonelMAGE21619363' 3417 8425 0. 94 2. 0E-11 AF020503. 1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydrolase (FHIT) gene, exon 5 4321 9313 1. 04 2. 0E-11 BE065537. 1 EST_HUMAN RC3-BT0316-170200-014-eO5 BT0316 Homo sapiens cDNA 4474 9464 0. 75 2. 0E-11 AL163227. 2 NT Homo sapiens chromosome 21 segment Hs21C027 4779 9763 1. 84 2. 0E-11 BE062558. 1 EST_HUMAN QV2-BT0258-261099-014-a01 BT0258 Homo sapiens cDNA 668 5693 10702 0. 79 1. OE-11 AJ131016. 1 NT Homo sapiens SCL gene locus 775 5797 10825 1. 24 1. 0E-11 AL163209.2 NT Homo sapiens chromosome 21 segment HS21 C009 1198 6199 11235 2. 94 1. 0E-11 AL163279. 2 NT Homo sapiens chromosome 21 segment HS21CO79 1469 6466 1. 4 1. 0E-11 AF119914. 1 NT Homo sapiens PRO3078 mRNA, complete cds 1986 6971 12075 1. 14 1.0E-11 P16258 SWISSPROT OXYSTEROL-BINDING PROTEIN 2067 7049 12157 2. 2 1. OE-11 AF000573. 1 NT Homo sapiens homogentisate 1, 2-dioxygenase gene, complete cds 3419 8427 13453 0. 93 1. OE-11 BE004315. 1 EST HUMAN CMO-BN0105-170300-292-d12 BN0105 Homo sapiens cDNA 4526 9516 14502 1. 42 7. 0E-12 Q05904 SWISSPROT 34 KD SPICULE MATRIX PROTEIN PRECURSOR (LSM34) 3471 8479 0.75 6.0E-12 AV730554. 1 EST_HUMAN AV730554 HTF Homo sapiens cDNA clone HTFAW F06 5' nz88f11. s1 NCfCGAPGCB1 Homo sapiens cDNA clone IMAGE : 1302573 3'similar to contains Alu 4222 9216 14195 9. 87 6. OE-12 AA732516. 1 EST HUMAN repetitive element ; 5074 10043 15009 1. 48 6. 0E-12 M22486. 1 NT Human chromosome 21 distal long arm DNA 1026 6036 11068 2. 25 5. 0E-12 T06573.1 EST_HUMAN EST04462 Fetal brain, Stratagene (cat#936206) Homo sapiens cDNA clone HFBDV33 3306 8317 13343 1. 16 5. OE-12 BE047779. 1 EST HUMAN tz42b05. y1 NCI CGAP Brn52 Homo sapiens cDNA clone IMAGE : 2291217 5' 3644 8650 13656 7.07 5.0E-12 AJ271736. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 2/2 242 5303 10313 4. 12 4. 0E-12 AA700326.1 EST_HUMAN zj74g11.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 460676 3' 243 5303 10313 6. 02 4. 0E-12 AA700326. 1 EST_HUMAN zj74g11.s1 Soares_fetal_liver_spleen_1NFLS-S1 Homo sapiens cDNA clone IMAGE : 460676 3' tx26h05.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE:2270745 3' similar to TR:Q13539 Q13539 4487 9477 14457 0.7 4.0E-12 AI689984.1 EST_HUMAN MARINER TRANSPOSASE.; Page 72 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Most Similar Top Hit Probe EX ORF SEQ Expression (Top) Hit Top Hit Acession, EQ ID a BLAST E N DSoubce Top Hit Descriptor Vaiue hd13d01. x1 Soares_NFL_T_GBC S1 Homo sapiens cDNA clone IMAGE : 2909377 3'similar to TR : 014597 NO : N : Value hd13d01. x1 Soares_NFL_T_GBC S1 Homo sapiens cDNA clone IMAGE : 299377 3'similar to TR : 014517 5639 10641 3. 43 3. OE-12 AW341683. 1 EST HUMAN 014517 SMRP. ; 612 612 5639 10642 3. 43 3. OE-12 AW341683. 1 EST HUMAN 014517 SMRP. ; 609 11673 1. 82 2. OE-12 AW 802131. 1 EST HUMAN IL5-UM0071-120400-065-a05 UM0071 Homo sapiens cDNA 13418 0. 75 2. OE-12 6754495 NT Mus musculus keratin-associated protein 6. 2 (Krtap6-2) mRNA 1613 6 3386 8394 3998 ggg4 13981 0. 85 2. OE-12 J01884. 1 NT Rat U3A small nuclear RNA 3998 ggg4 13982 0. 85 2. OE-12 J01884. 1 NT Rat U3A small nuclear RNA 9289 2. 34 2. OE-12 BE063509. 1 EST HUMAN CMO-BT0281-039199-087-a03 BT0281 Homo sapiens cDNA 4742 9727 14712 1. 72 2. OE-12 070306 SW ISSPROT TBX15 PROTEIN (T-BOX PROTEIN 15) 4297 hh90a09. x1 NCI_CGAP GU1 Homo sapiens cDNA clone IMAGE : 297040 3'similar to contains MER18. t1 4742 9727 14713 1. 72 2. OE-12 070306 SW ISSPROT TBX15 PROTEIN (T-BOX PROTEIN 15) wm51f07. x1 NCI_CGAP Ut2 Homo sapiens cDNA clone IMAGE : 2439493 3'similar to contains L1. b3 L1 123 5192 10205 1. 65 1. OE-12 AW 627674. 1 EST HUMAN MER18 repetitive element ; 1941 6927 1. 12 1. OE-12 AI871726. 1 EST HUMAN repeti6ve element ; 1. 34 1. OE-12 AF000991. 1 NT Homo sapiens testis-specific Testis TranscriptY 2 (TTY2) mRNA, partial cds 1. 34 1. OE-12 AF000991. 1 NT Homo sapiens testis-specific Testis Tanscript Y 2 (TTY2) mRNA, partial cds 2997 8015 13027 3gg 29. 33 1. OE-12 AU132248. 1 EST HUMAN AU132248 NT2RP3 Homo sapiens cDNA clone NT2RP3004070 5 2997 8015 13028 2g. g3 1. OE-12 AU132248. 1 EST HUMAN AU132248 NT2RP3 Homo sapiens cDNA ctone NT2RP3004070 5' 3783 8786 1. 53 9. OE-13 AJ271735. 1 NT Homo sapiens X9 Pseudoautosomal region ; segment 112 3783 8786 137 0. 85 9. OE-13 AB029900. 1 NT Homo sapiens CST gene for cerebroside sulfotransferase, exon 1, 2, 3, 4, 5 3543 8550 5731 10747 4. 84 8. OE-13 U29185. 1 NT Homo sapiens prion protein (PrP) gene, complete cds 3843 8845 13854 10748 4. 84 8. OE-13 U29185. 1 NT Homo sapiens prion protein (PrP) gene, completa cds Homo sapiens basic transcription factor 2 p44 (btf2p44) gene, partial cds, neuronal apoptosis inhibitory 707 - p7 5731 7 2. 36 8. OE-13 U80017. 1 NT protein (naip) and survival motor neuron protein (smn) genes, complete cds -3 AL163207. 2 NT Homo sapiens chromosome 21 segment HS21 C007 1802 6793 11883 0. 67 5. OE-13 R78338. 1 EST HUMAN N84. r1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 145759 5' 2044 7026 12137 3. 41 6. OE 1 zt77a12. s1 Soares testis_NHT Homo sapiens cDNA clone IMAGE : 728350 3'similar to contains Alu 3248 8261 36 5. OE-13 AA435773. 1 EST HUMAN repetitive element ; contains element MER22 repetitive element ; 9. 36 4. OE-13 AW 38614. 1 EST HUMAN PM2-HT0224-221099-001-e11 HT0224 Homo sapiens cDNA 3313 1. 32 4. OE-13 AF003529. 1 NT Homo sapiens glypican 3 (GPC3) gene, partial cds and flanking repeat regions 1830 6820 1. 02 4. OE-13 AA454054. 1 EST HUMAN d07. r1 Soares testis_NHT Homo sapiens cDNA clone IMAGE : 795469 5' Fiomo sapiens X tinked anhidroi6e ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeet 2391 7362 4604 9592 3. OE-13 AF003528. 1 NT regions Page 73 of 209 Tabie 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit BLAST E Database Top Hit Descriptor Probe Exon pRF SEQ Eression (Top) Hit Top Hit Acession SEQ ID SEQ ID ID NO : Signal No. N0 : N : Value Sourca 3. OE-13 AA43o310. 1 EST HUMAN 98r1 Soares testis_NHT Homo sapiens cDNA clone IMAGE : 781406 5' 12404 1. 24 3. OE-13 AJ271736. 1 NT Homo sPs Xq Psaudautosomal regiorc se9ment 212 854 5873 6. 84 2. 01 3. OE-13 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21 C010 2309 7284 3. 02 3. OE-13 BF372962. 1 EST HUMAN CM3-FT0100-140700-242-h08 FT0100 Homo sapiens cDNA 2408 3 3. OE-13 AA745844. 1 EST HUMAN ob18d02. s1 NCI_CGAP Kid5 Homo sapiens cDNA clone IMAGE : 1324035 3 Homo sapiens X28 reJion near ALD locs containing dual specoitY Phosphatase 9 (DUSP9), ribosomal 2593 7556 126 protein LlBa (RPL18a), Ca2+ICalmodulin-dependent protein kinase I (CAMKI), crea6ne transporter (CRTR), 13 8129 2. 06 31 10229 2. 57 2. OE-13 U52111. 2 NT CDM protein (CDM), adrenoleukodystroPhY Protein > _gg 2. OE-13 U23839. 1 NT Danio rerio fibroblast growth factor receptor 4 mRNA, complete cds 149 52 0. 1 NT Homo sapiens DNA polYreerase delta small subunit (POLD2) gene, exons 1 through 11 and compete cds 3 52gg 10310 OE-13 BF431899. 1 EST HUMAN nab76f05. x1 Soares_NSF F8 9W OT PA P S1 Homo sapiens cDNA clone IMAGE : 3' 1251 6249 11290 6. 43 2. OE-13 AF23971 Homo sapiens S164 gene, partial cds ; PS1 and hypoecal protein genes, compiete cds ; and S171 gene, 8 g3 13245 1. 32 32 3428 8436 13462 1. 88 2. OE-13 AF109907, 1 NT partial cds 2p2 2. OE-13 AL163278. 2 NT Homo sapiens chromosoma 21 segment HS21 C078 1. OE-13 S74129. 1 NT FGF-1=fibroblast gowth factr 1 [human, kidney, Genomio 342 nt, segment 2 af 2] 3992 8989 H. sapiens DMA, DMB, HLA-Z1 PP2 LMP2, TAP1, LMP7, TAP2 OB, DQB2 and RING8, 9, 13 and 14 Zgg 5346 10358 1. 09 g5 10936 4. 81 1. OE-13 AJ007973. 1 NT Homo sapiens LGMD2B gene 877 5$ _ nw21g02. s1 NCI_CGAP GCBO Homo sapiens cDNA clone IMAGE : 1241138 3'similar to contains THR. t3 13 11362 1. 45 1. OE-13 X87344. 1 NT genes 1316 63 6957 12062 1. 73 1. OE-13 AA720574. 1 EST HUMAN THR repetitive et ; 1. OE-13 BF340987. 1 EST HUMAN 602038009F1 NCI_CGAP Brn64 hiom sapiens cDNA clone IMAGE : 4185866 5' '4c01. s1 Soares testis_NHT Homo sapiens cDNA clone 1391232 3'similar to contains MER19. t1 MER19 a2 4461 9451 14432 1. 6- aj24c01_s1 Soaes testis_NHT Homo sapiens cDNA clone 1391232 3'similar to contains MER19. t1 MER19. gg 10391 2. 84 9. OE-14 AA781159. 1 EST HUMAN repeti6ve element ; 331 53 332 5384 10392 2. 83 9. OE-14 AA781159. 1 EST HUMAN repetitive element ; 3. 07 9. OE-14 AW a61577. 1 EST HUMAN RC4-CT0322-080100-013-d09 CT0322 Homo sapiens cDNA g. gg 9. OE-14 AB038162. 1 NT Homo sapiens TFF gene cluster for trefal factor, complete cds 2427 7398 9. OE-14 AW 513296. 1 EST HUMAN xo54h05. x1 NCI_CGAP Ut1 Homo saPiens cDNA clone IMAGE : 2707833 3' 2680 7638 1275 NT Human DNA, SINE repeti6ve element 13062 5. 01 NT Saguinus oediPus gene for seminal vesicle secreted protein semenogein I 3037 8054 13715 7. 31 9. OE-14 D14547. 1 3708 8712 8. OE-14 BE468263. 1 EST HUMAN hz71c09. x1 NCI_CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3213424 3', 4612 9598 14584 1. 69 9. OE-14 AJ002153. 1 8426 1. 04 EST HUMAN N72e03. r1 Soares piacenta Nb2HP Homo sapiens cDNA clone IMAGE : 144796 3' 3418 3849 8851 3. 5 8. OE-14 R76269. 1 t Page 74 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit Probe Exon pRF SEQ Expression (Top) Hit Top Hit Acession pabase, SEQ ID SEQ ID ID NO : Signal BLAST E Top Hit Descriptor NO : NO : N'Sourca Value xf67e10. x1 NCI_CGAP Gas4 Homo sapiens cDNA clona IMAGE : 2623146 3'similar to contains MER10. t2 1590 7751 2. 98 7. OE-14 AW 151673. 1 EST HUMAN MER10 repetitive element ; g_gg 6. OE-14 AF020503. 1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydrolase (FHIT) gene, exon 5 CANALICULAR MULTISPECIFIC ORGANIC ANION TRANSPORTER 1 (MULTIDRUG RESISTANCE- 366 5415 104 10644 4. 27 5. OE-14 Q63120 SW ISSPROT ASSOCIATED PROTEIN 2) (CANALICULAR MULTIDRUG RESISTANCE PROTEIN) 03b05 : x1 NCI_CGAP GU1 Homo sapiens cDNA clone IMAGE : 2575185 3'similar to contains L1. t2 L1 614 5641 4886 9865 14836 1. 45 5. OE-14 AW 073791. 1 EST HUMAN repetitive element ; 1106 7739 1. 59 4. OE-14 P04928 SW ISSPROT S-ANTIGEN PROTEIN PRECURSOR 1839 6829 11918 5. 27 4. OE-14 AJ007973. 1 NT Homo sapiens LGMD2B gene p. gg 4. OE-14 AA046502. 1 EST HUMAN zk67a06. r1 Soaresregnant uterus NbHPU Homo sapiens cDNA clone IMAGE : 487858 5' yy73c12. s1 Soares_multiple sclerosis 2NbHMSP Homo sapiens cDNA clone IMAGE : 279190 3'similar to 3666 8 4168 9163 14149 0. 8 4. OE-14 N46328. 1 EST HUMAN contains L1. t3 L1 repeti6ve element ; 935 5952 10984 1. 12 3. OE-14 X95466. 1 NT R. norvegicus mRNA for CPG2 protein 3gg 5427 10442 2. 33 2. OE-14 AJ271736. 1 NT Hamo sapiens Xq pseudoautosomal region se9ment 212 3gg 5427 10443 2. 33 2. OE-14 AJ271736. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 212 681 7727 10716 7. 63 2. OE-14 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 7298 1. 24 2. OE-14 AW 372868. 1 EST HUMAN RC5-BT0377-091299-031-D12 BT0377 Homo sapiens cDNA 7366 1. 08 2. OE-14 7657529 NT Homo sapiens rhabdoid tumor deletion region protein 1 (RTDR1), mRNA 2324 2455 7425 12539 1. 41 2. OE-14 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21 C009 2395 11088 1. 59 1. OE-14 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 1382 6379 11427 4. 61 1. OE-14 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21C068 1050 g 11428 4. 61 1. OE-14 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21 C068 Homo sapiens chromosome X region from filamin (FLN) gene to glucose-6-phosphate dehydrogenase 1382 637 1955 6941 12043 29. 53 1. OE-14 L44140. 1 NT (G6PD) gene, complete cds's 4. 33 1. OE-14 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 343 7317 12437 18 1. OE-14 AF001689. 1 NT Homo sapiens ibosomal protein L23A (RPL23A) gene oomPete cds 2121 7101 1221 7gg3 12916 1. 2 1. OE-14 P05227 SW ISSPROT HISTIDINE-RICH PROTEIN PRECURSOR (CLONE PFHRP-II) 2 . 5g 1. OE-14 BF335227. 1 EST HUMAN RC2-CT0432-310700-013-a09 1 CT0432 Homo sapiens cDNA 2$ 5_gg 1. OE-14 BF335227. 1 EST HUMAN RC2-CT0432-310700-013-a09 1 CT0432 Homo sapiens cDNA 3094 8110 13127 13800 1. 7 1. OE-14 AA682994. 1 EST HUMAN 912. s1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE : 971350 3 3094 8110 131 14320 1. 99 1. OE-14 AW 275852. 1 EST HUMAN xq39h10. x1 NCI_CGAP Lu28 Homo sapiens cDNA clone IMAGE : 2753059 3' 3792 8795 11596 1. 39 9. OE-15 7427522 NT Homo sapiens Protein tyrosine phosphatase, recePtor tYPe. T (PTPRT), mRNA 4348 9339 1541 6539 Page 75 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Most Similar Top Hit Probe EX pRF SEQ Expression (Top) Hit Top Hit Acession pabase SEQ ID SEQ I ID NO : Signal BLAST E No. Top Hit Descriptor NO : NO : Value Source Vaiue Homo sapiens transcription factor IGHM enhancer 3, JM11 protein, JM4 protem, JM5 protein, T54 protein, JM10 protein, A4 differenfia6on-dependent protein, triple LIM domain protein 6, and synaptophysin genes 2108 7088 1. 04 9. OE-15 AF196779. 1 NT complete cds ; and L-type calcium channel a> 5513 1. 02 S. OE-15 BE261482. 1 EST HUMAN 601148632F1 NIH MGC 19 Homo sapiens cDNA clone IMAGE : 3164023 5' g7g 5994 11027 4. 97 6. OE-15 AJ271736. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 2l2 2739 407 5444 10464 4. 86 5. OE-15 AL163208. 2 NT Homo sapiens chromosome 21 segment HS21 C008 Human hereditary haemochromatosis region, histone 2A-like protein gene, hereditary haemochromatosis 12759 1. 12 5. OE-15 U91328. 1 NT (HLA-H) gene, RoRet gene, and sodium phosphate transporter (NPT3) gene, complete cds 424 5082 10066 2. 43 4. OE-15 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 2686 7644 LY1142F Human fetal heart, Lambda ZAP Express Homo sapiens cDNA clone LY1142 5'similar to 4096 9090 7. 1 3. OE-15 N89452. 1 EST HUMAN ANF (CARDIODILATIN) 4770 9754 2. 28 3. OE-15 P92485 SW ISSPROT NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 5 Homo sapiens calcium channel alpha1 E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively Homo sapiens calcium channel alpha1E subunit (CACNA1E) gene, exons 7-49 and partial cds, alternatively 24g 5308 10319 2. 86 2. OE-15 AF223391. 1 NT spliced 367 5416 10429 2. 77 2. 0E-15 AF223391. 1 NT ; spticed Homo sapiens calcium channel alpha1 E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively 367 5416 10429 2. 77 2. OE-15 AF223391. 1 NT spliced ht09g01. x1 NCI_CGAP Kid13 Homo sapiens cDNA clone IMAGE : 3146256 3'similar to contains MER29. b3 3g7 5416 10430 2. 77 2. OE-15 AF223391. 1 NT spliced htO9g01. x1 NCI_CGAP Kid13 Homo sapiens cDNA clone IMAGE : 3146256 3'similar to contains MER29. b3 2311 7286 12406 1. 11 2. OE-15 BE350127. 1 EST HUMAN MER29 repetitive element ; Homo sapiens calcium channel alpha1E subunit (CACNA1E) gene, exons 7-49, and partial cds, alternatively 2311 7286 12407 1. 11 2. OE-15 BE350127. 1 EST HUMAN MER29 repetitive element ; Homo sapiens calcium channel alphalE subunit (CACNA1E) gene, exons 7-49, and partial cds, alterna6vely- 3430 8438 13464 0. 91 2. OE-15 AF223391. 1 NT spliced 26h01. x1 NCI_CGAP NN10 Homo sapiens cDNA clone IMAGE : 2741521 3'similar to contains L1. Y3 L1 3430 8438 13465 0. 91 2. OE-15 AF223391. 1 NT spliced wf07f06. x1 Soares_NFL_T_GBC S1 Homo sapiens cDNA clone IMAGE : 2349923 3'similar to TR : Q61043 3954 8952 13942 1. 02 2. OE-15 AW 238499. 1 EST HUMAN repetitive element ; 2. OE-15 AI806335. 1 EST HUMAN C161043 NINEIN. ; b<16h05. x1 NCI_CGAP Lu24 Homo sapiens cDNA clone IMAGE : 2270745 3'similar to TR : Q13539 Q13539 44, 2702 7659 1. 94 1. OE-15 AI689984. 1 EST HUMAN MARINER TRANSPOSASE. ; , 7g 1. OE-15 BE043584. 1 EST HUMAN hk40e02. y1 NCI_CGAP OY4 Homo sapiens cDNA clone IMAGE : 2999162 5 2943 7962 129 Page 76 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit Probe Exon pRF SEQ Expression (Top) Hit Top Hit Acession pabase SEQ ID SEQ ID p NO : Signal BLAST E Top Hit Descriptor No. gource NO : NO : Value 3069 8085 13099 1. 42 1. OE-15 P08547 SW ISSPROT LINE-1 REVERSE TRANSCRIPTASE HOMOLOG 4234 9228 14212 0. 99 1. OE-15 BE182696. 1 EST HUMAN RC3-HT0649-100500-022-b05 HT0649 Homo sapiens cDNA 4963 9940 14917 0. 93 1. OE-15 AI984928. 1 EST HUMAN wr86e04. x1 NCI_CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2494590 3' 2080 7061 6. 06 6. OE-16 AW 972611. 1 EST HUMAN EST384702 MAGE esequences, MAGL Homo sapiens cDNA 2_3 5. OE-16 AJ251154. 1 NT Mus musculus olfactory receptor cluster, OR37A, OR37B, OR37C, OR37E genes and OR37D pseudogene ot80c04. s1 Soares total fetus Nb2HF8_9w Hbmo sapiens cDNA clone IMAGE : 1623078 3'similar to 1461 6458 115 2609 7571 12685 1. 58 S. OE-16 AA992176. 1 EST HUMAN contains element L1 repefi6ve element ; p, gg 4. OE-16 AB001523. 1 NT Homo sapiens gene for TMEM1 and PW P2, complete and partial cds 2317 7292 12412 1. 01 4. OE-16 AW 797168. 1 EST HUMAN QV1-UM0036-200300-115-g02 UM0036 Homo sapiens cDNA 2317 7292 12413 1. 01 4. OE-16 AW797168. 1 EST HUMAN QV1-UM0036-200300-195-g02 UM0036 Homo sapiens cDNA 3375 8383 13403 4. 99 4. OE-16 Q16653 SWISSPROT MYELIN-OLIGODENDROCYTE GLYCOPROTEIN PRECURSOR 4019 9015 14002 4. 76 4. OE-16 BE083875. 1 EST HUMAN PM4-BT0650-010400-002-g09 BT0650 Homo sapiens cDNA 4019 9015 14003 4. 76 4. OE-16 BE083875. 1 EST HUMAN PM4-BT0650-010400-002-g09 BT0650 Homo sapiens cDNA 5199 10215 0. 97 3. OE-16 AW 022862. 1 EST HUMAN df45c01. yi Morton Fetal Cochlea Homo sapiens cDNA clone IMAGE : 2486376 5' 5199 10216 0. 97 3. OE-16 AW022862. 1 EST HUMAN df45c01. y1 Morton Fetal Cochlea Homo sapiens cDNA clone IMAGE : 2486376 5' 933 1. 75 3. OE-16 AL046445. 1 EST HUMAN DKFZp434P037 r1 434 (synonym : htes3) Nomo sapiens cDNA clone DKFZp434P037 5' 133 472 5508 2. 13 3. OE-16 AF135446. 1 NT Homo sapiens TSX (TSX) pseudogene, exon 5 463 5500 _ ENVELOPE GLYCOPROTEIN GP340 (MEMBRANE ANTIGEN) (MA) [CONTAINS : GLYCOPROTEIN 1426 6423 11482 3. 79 3. OE-16 Q28983 SW ISSPROT ZONADHESIN PRECURSOR 2944 4. 23 3. OE-16 P03200 SWISSPROT GP220] 8832 13839 0. 78 3. OE-16 T08169. 1 EST I-tUMAN EST06060 Infant Brain, Bento Soares Homo sapiens cDNA clone HIBBA13 5'end 3830 0. 67 3. oE-16 AF020503. 1 NT Homo sapiens FRA38 common fragile region, diadenosine triphosphate hydrolase (FHIT) gene, exon 5 9769 14753 1. 06 3. OE-16 AV661393. 1 EST HUMAN AV661393 GLC Homo sapiens cDNA clone GLCGSA01 3' 3851 8853 2. OE-16 AL163279. 2 NT Homo sapiens chromosome 21 segment HS21 C079 4785 2617 7579 0. 99 2. OE-16 J03061. 1 NT Human SSAV-related endogenous retrovirai LTR-like element 957 59 9048 14036 0. 92 2. OE-16 X89211. 1 NT H. sapiens DNA for endogenous retroviral like element 2. 54 1. OE-16 AF200719. 1 NT Homo sapiens pituitary tumor transforming gene protein (PTTG) 9ene, complete cds 4054 sf39g11. s1 Soares total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE : 1034084 3'similar to g 5247 10256 5456 25. 58 1. OE-16 AA628592. 1 EST HUMAN contains OFR. t2 OFR repe6tive element ; 12009 1. 7 1. OE-16 BF327942. 1 EST HUMAN QVO-BN014&070700-293-a10 BN0148 Homo sapiens cDNA 38 3. 23 9. OE-17 AW900048. 1 EST_HUMAN CM1-NN1003-200300-153-e01 NN1003 Homo sePiens cDNA 6913 1002 6012 1. 83 8. OE-17 AW 88701. 1 EST HUMAN (1V0-OT0032-080300-155-d01 OT0032 Homo sapiens cDNA 3654 8660 13665 Page 77 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Source NO: NO: Source Value 3797 8800 2.95 8.0E-17 AL163280.2 NT Homo sapiens chromosome 21 segment HS21C080 1431 64281. 93 7. 0E-17 6753097 NT Mus musculus apolipoprotein B edifing complex 2 (Apobec2), MRNA 203 5267 10281 5. 89 6. 0E-17 AW983880. 1 EST_HUMAN RC1-HN0003-220300-021-b04 HN0003 Homo sapiens cDNA 418 5076 10060 2.71 5. OE-17 T64110. 1 EST_HUMAN yc05h08.r1 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE : 79839 5' 3550 8557 13564 0. 94 4. 0E-17 AA643697. 1 EST HUMAN ni96e05. s1 NCI CGAP Co10 Homo sapiens cDNA clone IMAGE : 1058528 3' 2041 7023 12133 1. 06 3. 0E-17 AW119123. 1 EST_HUMAN xd89c09.x1 Soares_NFL-T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2604784 3' 3121 8137 1. 31 3. 0E-17 P35410 SWISSPROT MAS-RELATED G PROTEIN-COUPLED RECEPTOR MRG 3557 8564 13570 1. 36 3. 0E-17 BE326522. 1 EST_HUMAN hw05b04.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3181999 3' 3557 8564 13571 1. 36 3. 0E-17 BE326522. 1 EST_HUMAN hw05b04.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3181999 3' qt63a06.x1 NCI_CGAP_Eso2 Homo sapiens cDNA clone IMAGE : 1959922 3'similar to contains Alu 351 MM 10413 2. 52 2. 0E-17At270080. 1 EST HUMAN repetitive element ; qt63a06.x1 NCI_CGAP_Eso2 Homo sapiens cDNA clone IMAGE : 1959922 3' similar to contains Alu 352 5403 10413 2. 83 2. OE-17 AI270080. 1 EST_HUMAN repetitive element ; 973 599 2. 23 2. 0E-17 AA722932. 1 EST_HUMAN zh81d04.s1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone lMAGE : 399751 3' 2379 7351 12471 1. 92 2. OE-17 Q28983 SWISSPROT ZONADHESIN PRECURSOR 2379 7351 12472 1. 92 2. 0E-17 Q28983 SWISSPROT ZONADHESIN PRECURSOR NEUROFILAMENT TRIPLET H PROTEIN (200 KDA NEUROFILAMENT PROTEIN) (NEUROFILAMENT 2859 7879 12897 6. 43 2. 0E-17 P12036 SWISSPROT HEAVY POLYPEPTIDE) (NF-H) Homo sapiens X28 region near ALD locus containing dual specificity phosphatase 9 (DUSP9), ribosomal protein L18a (RPL 18a), Ca2+/Calmodulin-dependent protein kinase I (CAMKI), creatine transporter (CRTR), 3693 8697 4. 37 2. 0E-17 U52111. 2 NT CDM protein (CDM), adrenoleukodystrophy protein > 741 5764 10789 3. 03 1.0E-17 P08183 SWISSPROT MULTIDRUG RESISTANCE PROTEIN 1 (P-GLYCOPROTEIN 1) 167066660. 97 1.0E-17 AJ271736. 1 Homo sapiens Xq pseudoautosomal region ; segment 212 1730 6725 118034. 26 1.0E-17 AL163207. 2 NT Homo sapiens chromosome 21 segment HS21 C007 2057 7039 12148 1.51 1.0E-17 P02461 SWISSPROT COLLAGEN ALPHA 1 (Ill) CHAIN PRECURSOR 2274 7250 12367 2. 06 1.0E-17 U79410. 1 NT Homo sapiens thrombospondin 2 (THBS2) gene, promoter region and exons 1A and 1B Homo sapiens mannosidase, beta A, lysosomal (MANBA) gene, and ubiquitin-conjugating enzyme E2D 3 3488 8496 1. 05 1. 0E-17 AF224669. 1 NT (UBE2D3) genes, complete cds 4015 9011 8. 44 1.0E-17 R09942. 1 EST HUMAN yf30e07. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 128388 5' 3698 8702 13705 1. 84 8. OE-98 4758977 NT Homo sapiens protein tyrosine phosphatase, non-receptor type substrate 1 (PTPNS1)mRNA xx10b04.x1 NCI_CGAP_Pan1 Homo sapiens cDNA clone IMAGE : 2837071 3' similar to gb : L20868 60S 347 5399 10407 65. 43 7. 0E-18 AW316976. 1 EST_HUMAN RIBOSOMAL PROTEIN L4 (HUMAN) ; xx10b04.x1 NCI_CGAP_Pan1 Homo sapiens cDNA clone IMAGE : 2837071 3' similar to gb:L20868 60S 347 5399 10408 65. 43 7. 0E-18 AW316976. 1 ESI_HUMAN RIBOSOMAL PROTEIN L4 (HUMAN) ; Page 78 of 209 Table 4 Single Exon Probes Expressed Most Similar Top Hit gLAST E N Sou ce Top Hit Descriptor Probe ExonD ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ p NO : Signal NO : N Value 6. OE-18 X71791. 2 NT Rattus nave9icus partial GdnIPn-1 gene for glia-deriverJ nebnlprotease nepn I, enhancer region PROTEIN-GLUTAMINE GAMMA-GLUTAMYLTRANSFERASE (TISSUE TRANSGLUTAMINASE) 3221 8236 13257 1. 06 qm65g11. x1 Soareslacenta 8to9weeks 2NbHP8to9W Homo sapiens cDNA clone IMAGE : 1893668 3' 4603 9591 4. 28 6. OE-18 P52181 SW SSPROT (TGASE C) (TGC) 5. OE-18 AI280214. 9 EST_HUMAN similar to contains Alu repetitive element ; ho36h04. x1 NCI_CGAP Ut1 Homa saPens cDNA clone IMAGE : 3039511 3'similar to contains MER29. b3 130 6135 11166 11. 59 1 ho36h04x1 NCI_CGAP Ut1 Homo sapiens cDNA clone IMAGE : 3039511 3'similar to contains MER29. b3 125 5193 10206 1. 37 4. OE-18 BE044076. 1 EST HUMAN MER29 repe6tive element ; nq24f11. s1 NCI_CGAP Co10 Homo sapiens cDNA clone IMAGE : 1144845 3'similar to gb : M26326 g3 10207 1. 37 4. OE-18 BE044076. 1 EST HUMAN MER29 repetitive element ; 125 51 g 21. 73 4. OE-18 AA621814. 1 EST HUMAN KERATIN,'TYPE CYTOSKELETAL 18 (HUMAN) ; N-ACETY-LACTOSAMINIDE BETA-1, 6-N-ACETIGLUCOSAMINYLTRANSFERASE (N- 1678 6674 1174 p gg 4. oE-18 Q06430 SW ISSPROT ACETY-GLUCOSAMtNYLTRANSFERASE) (I-BRANCHING ENZYME) (IGNT) N-ACETYI-LACTOSAMINIDE BETA-1, 6-N-ACETY-GLUCOSAMINYLTRANSFERASE (N- 2138 7118 12232 1 g 4. OE-18 Q06430 SW ISSPROT ACETYLGLUCOSAMINYLTRANSFERASE) (1-BRANCHING ENZlNIE) (GNT) ob23h11. s1 NCI_CGAP Kid5 Homo sapiens cDNA clone IMAGE : 1324581 3'similar to SW : RS5_HUMAN g 7118 12233 0. 9 213 ggg 34. 38 3. OE-18 AA814196. 1 EST HUMAN P4678240S RIBOSOMAL PROTEIN S5. ; 10967 3. 2 3. OE-18 BE088634. 1 EST HUMAN CMO-BT0690-210300-298-g7 BT0690 Homo sapiens cDNA 8848 13856 1. 1 3. OE-18 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21 C047 5933 2. OE-18 AW 836820. 1 EST HUMAN QV1-LT0036-150200-070-e07 LT0036 Homo sapiens cDNA 3846 2. OE-18 BE256097. 1 EST HUMAN 601114352F1 NIH MGC 16 Homo sapiens cDNA clone IMAGE : 3355044 5' 24g 5309 10320 4. 3 13076 0. 93 2. OE-18 Q39575 SW ISSPROT DYNEIN GAMMA CHAIN, FLAGELLAR OUTER ARM y3g05. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 120536 5'similar to contains 1135 6140 211. 43 3050 8067 zt11d06. r1 NCI_CGAP GCB1 Homo sapiens cDNA clone IMAGE : 712811 5'similar to contains MER19. t2 0. 87 1. OE-18 T95406. 1 EST HUMAN L1 ePefi6ve element ; 4291 9283 zt11d06. r1 NCI_CGAP GCB1 Homo sapiens cDNA clone IMAGE : 712811 5'similar to contains MER19. t2 5576 10582 5. 62 9. OE-19 AA281961. 1 EST HUMAN MER19 repetitive element ; 541 3. 19 9. OE-19 AA281961. 1 EST HUMAN MER19 repetitive element ; -19 AW974902. 1 EST HUMAN EST387007 MAGE esequences, MAGN Homo sapiens cDNA 542 5576 1058 1031 6041 1. 33 8. OE 4758139 NT Homo saPens DEADM (Asp-Glu-Ala-AspIHis) box polYpePtide 6 (RNA helicase, 54kD) (DDX6) mRNA 1. 56 6. OE-19 AW 852930. 1 EST HUMAN PMO-CT0248-131099-001-g01 CT0248 Homo sapiens cDNA 2184 7163 12283 1. 86 7. OE-19 ; 9330 14314 1. 45 6. OE-19 P34986 SW ISSPROT OLFACTORY RECEPTOR 6 (M50) 3692 8696 9330 14315 1. 45 6. OE-19 P34986 SW ISSPROT OLFACTORY RECEPTOR 6 (M50) 4339 4339 Page 79 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORFSEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: NO: Source Value 4655 9640 1.38 6.0E-19 AJ271735.1 NT Homo sapiens Xq pseudoautosomal region; segment 1/2 4865 9845 148201 1. 45 6. 0E-19 AL120817. 1 EST_HUMAN DKFZp762F192_r1 762 (synonym : hmel2) Homo sapiens cDNA clone DKFZp762F192 5' 550 5584 10586 0. 91 4. 0E-19 AB007970. 1 NT Homo sapiens mRNA, chromosome 1 specific transcript KIAA0501 2611 7573 12687 1. 1 4. 0E-19 BF697362. 1 EST_HUMAN 602130910F1 NIH_MGC_56 Homo sapiens cDNA clone IMAGE : 4287674 5' 3762 8765 13767 0. 98 3. 0E-19 Q28997 SWISSPROT BETA-2 ADRENERGIC RECEPTOR 3762 8765 13768 0. 98 3. 0E-19 Q28997 SW ISSPROT BETA-2 ADRENERGIC RECEPTOR 4163 9158 14144 1. 07 3. 0E-19 043900 SWISSPROT LIM-ONLY PROTEIN 6 (TRIPLE LIM DOMAIN PROTEIN 6) 4163 9158 14145 1. 07 3. 0E-19 043900 SWISSPROT LIM-ONLY PROTEIN 6 (TRIPLE LIM DOMAIN PROTEIN 6) 4324 9316 14298 1. 25 3. 0E-19 AV708136. 1 ESTHUMAN AV708136ADC Homo sapiens cDNA clone ADCAMA11 6 2489 7457 12572 24. 97 2. 0E-19 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21C001 qo91e02. x1 NC) CGAPKid5 Homo sapiens cDNA clone IMAGE : 1915898 3'similar to TR : Q69386 Q69386 4323 9315 1. 37 2. 0E-19 AI311783. 1 EST HUMAN POLIENV GENE ; 478 5515 1. 56 1. OE-19 BE408611. 1 EST HUMAN 601304125F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3638310 5' yo79g07. r1 Soares adult brain N2b4HB55Y Homo sapiens cDNA clone IMAGE : 184188 5'similar to contains 2100 7081 12196 1. 18 1. OE-19 H30795. 1 EST HUMAN MER10 repetitive element ; 2645 7605 2. 01 1. OE-19 D38044. 1 NT Human gene for Ah-receptor, exon 7-9 2774 7795 5. 45 1. OE-19 4758977 NT Homo sapiens protein tyrosine phosphatase, non-receptor type substrate 1 (PTPNS1) mRNA aj49b12. s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE : 1393631 3'similar to contains MER37. t2 3317 8327 13348 1. 46 1. OE-19 AA834967. 1 EST HUMAN MER37 repetitive element ; 3202 8217 13240 0. 74 7. 0E-20 BF326455. 1 EST HUMAN PM4-AN0096-050900-003-a04 AN0096 Homo sapiens cDNA 3478 8486 13505 3. 52 6. 0E-20 P39188 SWISSPROT ALU SUBFAMILY J SEQUENCE CONTAMINATION WARNING ENTRY 4150 9145 14129 2. 85 6. 0E-20 BE622434. 1 EST_HUMAN 601441231F1 NIH_MGC_72 Homo sapiens cDNA clone IMAGE : 3916231 6' 4464 9454 1. 02 5. 0E-20 AV725123. 1 EST HUMAN AV725123 HTC Homo sapiens cDNA clone HTCBTA01 5' 1581 6578 11642 1. 44 4. 0E-20 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21C047 2079 7060 12170 1. 22 3. 0E-20 U03888. 1 NT Human BXP21 gene 4088 9082 14073 1. 46 3. 0E-20 P23273 SWISSPROT OLFACTORY RECEPTOR-LIKE PROTEIN 114 zk36b12. s1 SoarespregnantuterusNbHPU Homo sapiens cDNA clone IMAGE : 484895 3'similar to 4491 9481 14460 1. 27 3. 0E-20 AA037616. 1 EST HUMAN contains L1. Y3 L1 repetitive element ; xr24e10. x1 NCI_CGAP_Ut4 Homo sapiens cDNA clone IMAGE : 2761098 3'similar to SW : RS5MOUSE 820 5840 48. 46 2. 0E-20 AW303868. 1 EST HUMAN P97461 40S RIBOSOMAL PROTEIN S5. ; ng69h09. s1 NCI_CGAP_Lip2 Homo sapiens cDNA clone IMAGE : 940097 similar to TR : G1224066 1094 6101 11130 3.05 2. 0E-20 AA516335. 1 EST HUMAN G1224066 ORF2 : FUNCTION UNKNOWN. ; ng69h09. s1 NCLCGAPLip2 Homo sapiens cDNA clone IMAGE : 940097 similar to TR : G1224066 109 6101 11131 3.05 2. 0E-20 AA516335. 1 EST HUMAN G1224066 ORF2 : FUNCTION UNKNOWN. ; Page 80 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Tap) Hit Top Hit Acession pabase Top Hit Descriptor NO : NO : ID NO : Signal BLAST E N Source. Value 24e10. x1 NCI_CGAP Ut4 Homo sapiens cDNA clone IMAGE : 2761098 3'similar to SW : RS5 MOUSE 2745 5840 36. 55 2. OE-20 AW 303868. 1 EST HUMAN P97461 40S RIBOSOMAL PROTEIN S5. ; 4789 9773 14756 4. 58 2. OE-20 Q28983 SW ISSPROT ZONADHESIN PRECURSOR 47gg 9773 14757 4. 58 2. OE-20 Q28983 SWISSPROT ZONADHESIN PRECURSOR 4969 9946 1. 73 2. OE-20 5174538 NT Homo sapiens malate dehydrogenase 1, NAD (saluble) (MDH1) mRNA zt11d06. r1 NCI_CGAP GCB1 Homo sapiens cDNA clone IMAGE : 712811 5'similar to contains MEFt19. t2 1963 7703 12051 2. 7 1. OE-20 AA291961. 1 EST HUMAN MER19 repefitive element ; hr84b06. x1 NCI_CGAP Kid11 Homo sapiens cDNA clone IMAGE : 3135155 3'similar to contains L1. t2 L1 4316 9308 14293 1. 12 1. OE-20 BF115158. 1 EST HUMAN epetitive element ; 2842 7862 1. 16 9. OE-21 AJ003514. 1 EST HUMAN AJ003514 Selected chromosome 21 cDNA library Homo sapiens cDNA clone MPIpll2-8J21 2016 6999 12102 1. 61 7. OE-21 P15800 SW ISSPROT LAMININ BETA-2 CHAIN PRECURSOR (S-LAMININ) (LAMININ CHAIN B3) 2016 6999 12103 1. 61 7. OE-21 P15800 SW ISSPROT LAMININ BETA-2 CHAIN PRECURSOR (S-LAMININ) (LAMININ CHAIN B3) 4133 9128 6. 12 7. OE-21 AA046502. 1 EST HUMAN 7Jc67a06. r1 Soaresregnant uterus NbHPU Homo sapiens cDNA cione IMAGE : 487858 5' 3gg7 gg85 13971 0. 98 6. OE-21 BE408611. 1. EST HUMAN 601304125F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3638310 5' 913 5929 10964 0. 79 5. OE-21 5902031 NT Homo sapiens protein tyrosine phosphatase, non-receptor type 21 (PTPN21), mRNA 4238 9232 14215 3. 34 5. OE-21 BE968839. 1 EST NUMAN 601649871 F1 NIH_MGC_74 Homo sapiens cDNA clone IMAGE : 3933880 5' 4548 5929 10964 0. 83 5. OE-21 5902031 NT Homo sapiens pratein tyrosine phosphatase, non-receptor type 21 (PTPN21), mRNA 4657 9642 14631 6. 35 5. OE-21 4885474 NT Homo sapiens melanoma antigen, family C, 1 (MAGEC1), mRNA oo86e08. s1 NCI_CGAP Kid5 Homo sapiens cDNA clone IMAGE : 1573094 3'similar to TR : G116530 Q16530 y 1696 6691 11767 1. 66 4. OE-21 AA970713. 1 EST HUMAN PMS3 MRNA ; contains OFR. t1 OFR repetitive element ; 1801 6792 11882 1. 05 3. OE-21 AA218891. 1 EST HUMAN zq15d06. s1 Stratagene fetal retina 937202 Homo sapiens cDNA clone IMAGE : 629771 3' 2212 7189 12310 0. 99 3. OE-21 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21 C001 3006 8023 13035 3. 56 3. OE-21 AJ007973. 1 NT Homo sapiens LGMD2B gene 145 5211 18. 78 2. OE-21 BE163247. 1 EST NUMAtJ QV3-NT0458-170200-090-g12 HT0458 Homo sapiens cDNA 922 5938 10969 0. 87 2. OE-21 AB007857. 2 NT Homo sapiens mRNA for KIAA0397 protein, partial cds 922 5938 10970 0. 87 2. OE-21 AB007857. 2 NT Homo sapiens mRNA for KIAA0397 protein, partial cds 1195 6196 2. 36 2. OE-21 BE064410. 1 EST HUMAN RC4-BT0311-141199-011-h06 BT0311 Homo sapiens cDNA 2567 7530 12648 2. 35 2. oE-21 Q28983 SWISSPROT ZONADHESIN PRECURSOR 2567 7530 12649 2. 35 2. OE-21 Q28983 SWISSPROT ZONADHESIN PRECURSOR n146c04. s1 NCI_CGAP Pr4 Homo sapiens cDNA clone IMAGE : 1043718 similar to contains MER29. b2 _ z 1236 6234 11278 1. 4 1. OE-21 AA557657. 1 EST HUMAN MER29 repetiBve element ; _ 1378 6375 10. 68 1. OE-21 A (601264. 1 EST HUMAN ar88d12. x1 Barstead colon HPLRB7 Homo sapiens cDNA clone IMAGE : 2152343 3'_ tz94a03. x1 NCI_CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2296204 3'similar to TR : Q15408 Q15408 4286 9278 14266 1. 11 9. OE-22 AI702438. 1 EST HUMAN NEUTRAL PROTEASE LARGE SUBUNIT ; _ e Page 81 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit ORF SEQ Eression (Top) Hit Top Hit Acession pabase Top Hit Descriptor SEQ ID SEQ ID p NO : Signal BLAST E No.. Source NO : N0 : Value 934 5951 5. 16 8. OE-22 BE144748. 1 EST HUMAN CMO-HT0179-281099-076-h05 HT0179 Homo sapiens cDNA 657 5684 10691 4. 6 7. OE-22 AL163246. 2 NT Homo sapis chromosome 21 segment HS21 C046 4160 9155 14138 2. 11 7. OE-22 Q61838 SWISSPROT ALPHA-2-MACROGLOBULIN PRECURSOR (ALPHA2M) 4g2 gg5 14826 0. 98 7. OE-22 AB008681. 1 NT Homo sapiens gene for ac6vin receptor type IIB, complete cds 3551 8558 13. 85 4. OE-22 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 112 tm14h10. x1 NCI_CGAP Co14 Homo sapiens cDNA clone IMAGE : 2156611 3'similar to gb : L19593 HIGH 944 5961 0. 98 3. OE-22 AI469679. 1 EST HUMAN AFFINITY INTERLEUKIN-8 RECEPTOR B (HUMAN) ; contains Ll. t1 L1 repetitive element ; w166b04. x1 NCI_CGAP Brn25 Homo sapiens cDNA clone IMAGE : 2429839 3'similar to SW : RL21 HUMAN 2495 7463 12578 2. 28 3. OE-22 AI859038. 1 EST HUMAN P46778 60S RIBOSOMAL PROTEIN L21. ; 3588 8595 1. 25 3. OE-22 D14718. 1 NT Human chromosomal protein HMG1 related gene qb28c07. x1 Soaresregnant uterus NbHPU Homo sapiens cDNA clone IMAGE : 1697580 3'similar to 4656 9641 14630 3. 17 3. OE-22 A1090125. 1 EST HUMAN contains MER12. t2 MER12 repetitive element ; 1913 6899 2. 09 2. OE-22 N24942. 1 EST_HUMAN yx73d05. s1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 267369 3' 2452 7422 12537 1. 44 2. OE-22 P24916 SW ISSPROT IMMEDIATE EARLY GENE 13 PROTEIN PRECURSOR 3335 8345 13363 3. 9 2. OE-22 8394043 NT Homo sapiens protein kinase, AMP-acfivated, gamma 3 non-catalytic subunit (PRKAG3), mRNA 4104 9098 14084 1. 53 2. OE-22 AW 817794. 1 EST_HUMAN PM1-ST0262-261199-001-d12 ST0262 Homo sapiens cDNA 1840 6830 11919 1. 41 1. OE-22 AW 865517. 1 EST HUMAN PM4-SN0020-010400-009-h02 SN0020 Homo sapiens cDNA 2507 7475 12590 1. 47 1. OE-22 U50871. 1 NT Human familial Alzheimer's disease (STM2) gene, complete cds 3326 8336 13356 1. 7 1. OE-22 D14547. 1 NT Human DNA, SINE epeti6ve element 3238 8251 2. 43 7. OE-23 AV647246. 1 EST HUMAN AV647246 GLC Homo sapiens cDNA clone GLCAW C07 3' 3349 8358 1. 59 6. OE-23 AF199333. 1 NT Rattus norvegicus RIM1B (Rim1B) mRNA, complete cds 4146 9141 14125 1. 04 6. OE-23 AL163249. 2 NT Homo sapiens chromosome 21 segment HS21 C049 5055 8358 1. 07 6. OE-23 AF199333. 1 NT Rattus norvegious RIM1B (Rim1B) mRNA, complete cds 658 5685 10692 3. 39 2. OE-23 AJ289880. 1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and LZTFL1. gene 1125 7697 3. 75 2. OE-23 M55270. 1 NT Human matrbc Gla protein (MGP) gene, compiete cds 2723 7680 12793 1. 37 2. OE-23 P22105 SW ISSPROT TENASCIN-X PRECURSOR (TN-X) (HEXABRACHION-LIKE) 2723 7680 12794 1. 37 2. OE-23 P22105 SW ISSPROT TENASCIN-X PRECURSOR (TN-X) (HEXABRACHION-LIKE) qs73f11. x1 NCI_CGAP Pr28 liomo sapiens cDtJA clana IMAGE : 1943757 3'similar to TR : Q13537 Q13537 300 1. 07 2. OE-23 AI201458. 1 EST HUMAN MER37 TRANSPOSABLE ELEMENT, COMPLETE CONSENSUS SEQUENCE. ; 3634 8640 4. 12 2. OE-23 BE165980. 1 EST_HUMAN MR3-HT0487-150200-113-g01 HT0487 Homo sapiens cDNA 3289 8 g7p 13874 2. 39 2. OE-23 H59931. 1 EST HUMAN yr16a02. r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 205418 5' 3869 887 13875 2. 39 2. OE-23 H59931. 1 EST HUMAN yr16a02. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE | 205418 5' 3869 8 4397 9388 14371 1. 16 1. OE-23 AL163252. 2 NT Homo sapiens chromosome 21 segment HS21C052 4620 9605 4. 78 1. OE-23 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21 C010 a Page 82 of 209 Table 4 Single Exon Probes Expressed Most Similar Top Hit Probe Exon pRF SEQ Expression (Top) Hit Top Hit Acession SEQ ID na BLAST E N DSoubce TP Hit Descriptor SEQ ID p NO : S9 ab75a08. s1 Stratagene fetal retina 937202 Homo sapiens cDNA cione IMAGE : 852758 3'similar to NO : N Value 548 5582 2. 52 9. OE-24 AA663213. 1 EST HUMAN TR : E19822 E19822 CA PROTEIN. ; 4513 9503 14482 1. 13 B. OE-24 P23269 SW ISSPROT OLFACTORY RECEPTOR-LIKE PROTEIN 13- 4513 9503 14483 1. 13 8. OE-24 P23269 SW ISSPROT OLFACTORY RECEPTOR-LIKE PROTEIN 13 784 0_g3 7. OE-24 AW 937954. 1 EST HUMAN QVO-DT0047-170200-122-a06 DT0047 Homo sapiens cDNA 7. OE-24 AL039498. 1 EST HUMAN DKFZp434A2311 r1 434 (synonym : htes3) Homo saPiens cDNA clone DKFZp434A2311 5' 3781 $ 2, 3 6. OE-24 AB001421. 1 NT Macaca fuscata mRNA for Testis-Specific Protein Y (TSPI, completa cds 4983 9988 1. 18 g 5848 10885 33. 51 6. OE-24 AL163249. 2 NT Homo sapiens chromosome 21 segment H$21 C049 696 5720 S. OE-24 AJ22943_1 NT Homo sapiens 959 kb contig beiween AML1 and CBR1 on chromosome 21q22, segment 313 82 1. 85 3. OE-24 F08337. 1 EST HUMAN HSCZRC061 normalized infant brain cDNA Homo sapiens cDNA clone c-uc06 3863 8865 13869 8. 31 , g 2. OE-24 AA167539. 1 EST HUMAN zP11f09. 1 Stratagene fetal re6na 937202 Homo sapiens cDNA cione IMAGE : 609161 5' 4919 9897 14871 p_7g 2. OE-24 AW898189. 1 EST HUMAN RC3-NN0068-09500-021-b03 NN0068 Homo sapiens cDNA 2285 7261 12379 6653 11725 2. 5 1. OE-24 7706340 NT Homo sapiens CGI-127 protein (LOC51646), mRNA 3709 8713 1. 1 1. OE-24 AW 82194. 1 EST HUMAN QVO-ST0294-100400-185-c10 ST0294 Homo sapiens cDNA 1657 1. OE-24 D86423. 1 NT Mus musculus mRNA for HGT keratin, partial cds 2602 7564 ne92e10. s1 NCI_CGAP Kid1 Homo sapiens cDNA clone IMAGE : 911754 similar to contains MER1. b2 4148 9143 1. 8 1. OE-24 AF143313. 1 NT Homo sapiens PTEN (PTEN) gene, exon 2 2954 4gg 3. 17 7. OE-25 AA483944. 1 EST HUMAN MER1 repe6tive element ; 11672 1. 2 5. OE-25 AW 850271. 1 EST HUMAN IL3-CT0219-161199-031-D04 CT0219 Homo sapiens cDNA 48 . OE-25 T98107. 1 EST HUMAN Ye56h04. r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 121783 5' 1612 6608 4. OE-25 AW $$7671. 1 EST HUMAN PM3 OT0093-280200-001-g07 OT0093 Homo sapiens cDNA 1421 6418 11478 1. 4- 3. 14 4. OE-25 BE170957. 1 EST HUMAN QV3-HT0543-140400-149-e11 HT0543 Homo sapiens cDNA 3318 8328 2. 89- 3. OE-25 8923321 NT Homo sapiens hypoetical protein FLJ20344 (FLJ20344), 4190 9183 mRNA 13281 3. 16 3. OE-25 8923321 NT Homo sapiens hypothetical protein FLJ20344 (FLJ20344), mRNA 3246 8259 1328 3. 49 2. OE-25 5032158 NT Homo sapiens transducin (beta)-like 1 (TBL1) mRNA 601511530F1 NIH MGC 71 Homo sapiens cDNA clone IMAGE : 3913087 5' 3246 8259 6327 11375 g. EST HUMAN 1329 2245 7222 12341 6. 05 2. OE-25 BE88801 7ep 12556 9. 38 2. oE-25 P17008 SW ISSPROT 40S RIBOSOMAL PROTEIN S16 60 14047 1. 69 2. OE-25 P17008 SW ISSPROT 40S RIBOSOMAL PROTEIN S16 2758 p 14048 1. 69 2. OE-25 P17008 SW ISSPROT 40S RIBOSOMAL PROTEIN S16 EST HUMAN DKFZp434H0313 r1 434 (synonym : htes3) Homo sapiens cDNA cione DKFZp434H0313 5' 4066 4066 906 363 5412 10425 0. 75 1. OE-25 AL040229. 1 2. 15 1. OE-25 9635487 NT Human endogenous retovirus, complete genome OT ATP SYNTHASE LIPID-BINDING PROTEIN P2 PRECURSOR (ATPASE PROTEIN 9) (SUBUNIT C) 1229 6228 2367 7341 12458 1. 69 1. OE-25 Q06055 SW ISSPR 14679 2. 8 1. OE-25 BE162737. 1 EST HUMAN PM1-HT0454-080100-002-h09 HT0454 Homo sapiens cDNA 383 12502 1. 14 9. OE-26 AL163218. 2 NT Homo sapiens chromosome 21 segment HS21 C018 4710 9695 2412 Page 83 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit Probe Exon ression (Top) Hit Top Hit Acession patabase SEQ ID SEQ ID RF SEQ E Top Hit Descriptor ID NO : Signal BLAST E N Source NO : NO : Value Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat 1542 6540 91597 1. 66 7. OE-26 AF003528. 1 NT regions 3873 8874 13879 1. 32 7. OE-26 X89211. 1 NT H. sapiens DNA for endogenous retroviral like element 4018 1. 76 7. OE-26 AW340153. 1 EST HUMAN hd02e12. x1 Soares_NFL_T_GBC S1 Homo sapiens cDNA clone IMAGE : 2908366 3' 4035 9031 g, p2 6. OE-26 AF029308. 1 NT Homo sapiens chromosome 9 duplication of the T cell receptor beta locus and trypsinogen gene families , 7g 6. OE-26 AA206131. 1 EST HUMAN zq52h04. r1 Stratagene neuroepithelium (#t937231) Homo sapiens cDNA clone IMAGE : 645271 5'_ 2164 7143 12261 as38h08. x1 Barstead aorta HPLRB6 Homo sapiens cDNA clone IMAGE : 2319519 3'similar to 3271 8283 13306 as38h08. x1 Barstead aorta HPLRB6 Homo sapiens cDNA clone IMAGE : 2319519 3'similar to 1158 6162 91196 5. 17 5. OE-26 AI708235. 1 EST HUMAN WP : F49C12. 11 CE03371 ; 1158 6162 11197 5. 17 5. OE-26 AI708235. 1 EST HUMAN W P : F49C12. 11 CE03371 ; 1721 6716 11792 1. 25 3. OE-26 D14547. 1 NT Human DNA, SINE repetitive element zn30d08. r1 Stratagene neuroepithelium NT2RAMI 937234 Homo sapiens cDNA clone IMAGE : 548943 5' Z. 2g 3. OE-26 AA115895. 1 EST HUMAN similar to gb : M14338 VITAMIN K-DEPENDENT PROTEIN S PRECURSOR (HUMAN) ; zo30f10. r1 Stratagene colon (3204) Homo sapiens cDNA clone IMAGE : 588427 5'similar to TR : G695374 1981 6966 13697 1. 2 3. OE-26 AA152464. 1 EST HUMAN G695374 TH1R01 RECEPTOR INTERACTOR ; zo30f10. r1 Shatagene colon (#937204) Homo sapiens cDNA clone IMAGE : 588427 5'similar to TR : G695374 3691 8695 13698 1. 2 3. OE-26 AA152464. 1 EST HUMAN G695374 THYRDD RECEPTOR INTERACTOR ; 673 5698 10707 5. 99 2. OE-26 AL163282. 2 NT Homo sapiens chromosome 21 segment HS21 C082 3691 8695 2. OE-26 AL038099. 2 EST HUMAN DKFZp566L171 s1 566 (synonym : hflcd2) Homo sapiens cDNA clone DKFZp566L171 3' 99 5. 27 2. OE-26 X86694. 1 NT M. musculus mRNA for astrocyHc phosphoprotein, PEA-15 1831 6821 2. 34 137 5203 10218 58. 33 1. OE-26 BE170371. 1 EST HUMAN QV4-HT0538-020300-123-a02 HT0538 Homo sapiens cDNA 3160 8176 131 p 12575 1. 04 1. OE-26 BE814995. 1 EST HUMAN MR2-BN0114-240500-030-g07 BN0114 Homo sapiens cDNA 57. 21 1. OE-26 AF261085. 1 NT Homo sapiens glyceraldehyde-3-Phosphate dehydrogenase (GADPH) mRNA, complete cds wj49c04. x1 NCI_CGAP Lu19 Homo sapiens cDNA clone IMAGE : 2406150 3'similar to contains THR. b2 2492 746 2615 7577 11 5091 10075 2. 7 8. OE-27 AI831462. 1 EST HUMAN THR repe6tive element ; 553 5587 2. 73 8. OE-27 AL163227. 2 NT Homo sapiens chromosome 21 segment HS21 C027 au87h08. x1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2783295 3'similar to gb : K00558 _ 11440 83. 09 8. OE-27 AW 162737. 1 EST HUMAN TUBULIN ALPHA-1 CHAIN (HUMAN) ; au87h08. x1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2783295 3'similar to gb : K00558 =. 6388 1391 1391 6388 11441 83. 09 S. OE-27 AW 162737. 1 EST_HUMAN TUBULIN ALPHA-1 CHAIN (HUMAN) ; 12199 1. 71 8. OE-27 AW 864776. 1 EST HUMAN PM2-SN0018-220300-002-a07 SN0018 Homo sapiens cDNA ADP, ATP CARRIER PROTEIN, LIVER ISOFORM T2 (ADPIATP TRANSLOCASE 3) (ADENINE 2103 7083' 3147 2. 66 S. OE-27 P12236 SW ISSPROT NUCLEOTIDE TRANSLOCATOR 3) (ANT 3) 3111 Page 84 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Most Similar Top Hit Probe Exon pRF SEQ Eression (Top) Hit Top Hit Acession pabase, SEQ ID SEQ ID ID NO : Signal BLAST E Top Hit Descriptor Source NO : N Value 675 5700 1. 35 7. OE-27 Z70664. 1 NT Human endo9enous etroviral element HC2 hi51h12. x1 Soares_NFL_T_GBC S1 Homo sapiens cDNA clona IMAGE : 2975879 3'similar to TR : 076040 4918 9896 2. 5 7. OE-27 AW629172. 1 EST HUMAN 076040 ORF2 : FUNCTION UNKNOWN. ; 1990 6975 12os0 2. 62 3. oE-27 X60658. 1 NT R. rattus RYA3 mRNA for a potential ligand-binding protein 4149 9144 14128 1. 26 3. OE-27 BE071924. 1 EST HUMAN PMO-BT0527-090100-001-d11 BT0527 Homo sapiens cDNA 42 522 10110 36. 86 2. OE-27 AF054187. 1 NT Homo sapiens alpha NAC mRNA, complete cds nk01b10. s1 NCI_CGAP Pr11 Homo sapiens cDNA clone IMAGE : 1000699 similar to gb : M17886 60S 1855 6844 37. 38 2. OE-27 AA565345. 1 EST HUMAN ACIDIC RIBOSOMAL PROTEIN P1 (HUMAN) ; hi51h12. x1 Soares_NFL T GBC S1 Homo sapiens cDNA clone IMAGE : 2975879 3'similar to TR : 076040 3036 8053 10. 93 2. OE-27 AW629172. 13189 1. 99 2. OE-27 AF111167. 2 NT Homo sapiens jun dimerization Protein gene, partial cds ; cfos gene comPete cds ; and unknown gene 3153 8169 13190 1. 99 2. OE-27 AF111167. 2 NT Homo sapiens jun dimerization protein gene, partial cds ; cfos gene, complete cds ; and unknown gene 433 5471 1. 55 1. OE-27 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 3153 8169 Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 1l2 (DLEC1, ORCTL3, ORCTL4 genes, 981 5996 11028 1. 2 1. 0E-27 AB026898. 1 Tcomptete cds) htO9g01. x1 NCI_CGAP Kid13 Homo sapiens cDNA clone IMAGE : 3146256 3'similar to contains MER29. b3 11028 1. 2 1. OE-27 AB026898. 1 NT campiete cds) hw17c11. x1 NCI_CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3183188 3'similar to TR : Q07314 Q07314 s 3966 8964 1. 02 1. OE-27 BE350127. 1 EST HUMAN MER29 repetitive element ; 2. 16 9. OE-28 BE348399. 1 EST HUMAN SECRETED NEUREXIN III-ALPHA-C PRECURSOR. [3j TR : Q07280 TR : Q07313 ; 309 5364 10375 2. 37 9. OE-28 AU126260. 1 EST HUMAN AU126260 NT2RP1 Homo sapiens cDNA clone NT2RP1000443 5' 141 5206 6 66 11200 15. 2 7. OE-28 AU142750. 1 EST HUMAN AU142750 Y79AA1 Homo sapiens cDNA clone Y79AA1000824 5' wo98c07. x'1 NCI_CGAP Pan1 Homo sapiens cDNA clone IMAGE : 2455692 3'similar to contains THR. b1 1162 316 5371 2. 32 5. OE-28 AI921003. 1 EST HUMAN THR repetitive element ; . 66 5. OE-28 R79762. 1 EST HUMAN 8O. r1 Soares piacenta Nb2HP Homo sapiens cDNA clone IMAGE : 146443 5' m33c09. x1 NCI_CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2695504 3'similar to SW : GG95_HUMAN 3902 8902 13900 2552 7517 12635 2. 12 4. OE-28 AW 195066. 1 EST HUMAN 18379 GOLGIN-95. ; 1. 09 4. OE-28 4505316 NT Homo sapiens myosin phosphatase, target subunit 1 (MYPT1), mRNA 052 13069 5. 95 4. OE-28 BE409100. 1 EST HUMAN 601300703F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3635305 5' 2908 7927 12945 Homo sapiens metalloprotease-like, disintegrin-like, cysteinrich potein 2 epsilon (ADAM22) mRNA, 3035 8 1264 6262 1. 66 3. OE-28 AF155382. 1 NT complete cds g7 5164 10175 8. 92 2. DE-28 BE062167. 1 EST HUMAN RC1-BT0254-220300-019-c05 BT0254 Homo sapiens cDNA 6151 11183 10. 35 2. OE-28 Y11107. 3 NT Homo sapiens ITGB4 gene for integrin beta 4 subunit, exons 3-41 1147 Page 85 of 209 Table 4 Single Exon Probes Expressed in Most Similar Top Hit gLAST E N DSource Top Hit Descriptor Probe EX ORF SEQ Eression (Top) Hit Top Hit Acession. SEQ ID SEQ ID NO : Signal qo35b06. x1 NCI_CGAP Lu5 Homo sapiens cDNA clone IMAGE : 1910483 3'similar to contains L1. b2 L1 NO : N : Value 2405 7376 12496'1. 68 2. OE-28 AI348634. 1 EST HUMAN » bti ent ; 13318 0. 68 2. OE-28 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21 C009 6445 11504 1. 71 1. OE-28 D38044. 1 NT Human gene for Ah-receptor, exon 7'9 3281 8293 2. 04 1. OE-28 BF333236. 1 EST HUMAN QV1-BT0821-120900-360 b03 BT0821 Homo sapiens cDNA 1448 1. OE-28 U09410. 1 NT Human zinc finger protein ZNF131 mRNA, partial cds 2158 7137 12256 gg74 14949 1. 55 1. oE-28 P23275 SW ISSPROT OLFACTORY RECEPTOR 15 (OR3) 4436 9426 1. 17 7. OE-29 AW966447. 1 EST HUMAN EST378521 MAGE resequences, MAGI Hamo sapiens cDNA 5003 7. OE-29 BE254708. 1 EST HUMAN 6011149F1 NIH MGC_16 Homo sapiens cDNA clone IMAGE : 3355367 5' 0 g r 3sE3 m0r bTR : 016476 \ 8484 1. 34 w 69b01. x1 NCI_CGAP Brn25 Homo sapiens cDNA clone IMAGE : 2466985 3'similar to TR : 015475 1567 6564 11627 P OE-29 AI936748. 1 EST HUMAN 015475 UNNAMED HERV-H PROTEIN ; contains LTR7. b1 LTR7 epetitive element ; 3476 1. 37 5. OE-29 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 590 5621 10620 9. 68 6. EST HUMAN cn15c02. x1 Normal Human Trabecular Bone Cells Homo sapiens cDNA clone NHTBC cn15c02 andom 4839 9823 3. OE-29 AB042297. 1 NT Homo sapiens PTS gene fw 6-PY'uvoyltetrahydroPterin synthase, complete cds 3161 8177 1. 75 4. OE-29 A1752367. 1- 1. 15 2. OE-29 AF084869. 1 NT Homo sapiens envelope protein RIC-6 (env) gene, complete cds 4290 9282 14270 1. 29 1. 15 2. OE-29 AF084869. 1 NT Homo sapiens envelopa protein RIC-6 (env) gene comPete cds wr65d10. x1 NCI_CGAP Ut1 Homo sapiens cDNA clone IMAGE : 2492563 3'similar to TR : 015546 015546 489 5525 10532 10533 qgg 5525 w65d10. x1 NCI_CGAP Ut1 Homo sapiens cDNA clone IMAGE : 2492563 3'similar to TR : 015546 015546 11553 4. 66 2. OE-29 AI963604. 1 EST HUMAN HERV-E ENVELOPE GLYCOPROTEIN ; 1501 6499 4. 66 2. OE-29 AI963604. 1 EST HUMAN HERV-E ENVELOPE GLYCOPROTEIN ; 4134 2. 09 2. OE-29 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21 C068 1501 6499 11554 1. 34 7. OE-30 BE091133. 1 EST HUMAN PM4-BT0724-150400-004-d11 BT0724 Homo sapiens cDNA 4157 9152 p. g 6. OE-30 X51755. 1 NT Human lambda-immunoglobulin constant region complex (germline) 1484 6481 6. OE-30 D25303. 1 NT Human mRNA for integin alpha subunit, complete cds 1534 6532 -0 BE008026. 1 EST HUMAN C1V0-BN0147-290400-214-f12 BN0147 Homo sapiens cDNA 36 6731 11808 1. 1 17- 0. 93 6. OE-30 BEO08o26. 1 EST HUMAN QVO-BN0147-290400-214-f12 BN0147 Homo sapiens cDNA tgg2g03. x1 NCI_CGAP CLL1 Nomo sapiens cDNA clone IMAGE : 2116276 3'simitar to contains Alu 3117 8133 13152 2. 42 6. OE 3 8133 13152 4614 3906 8906 13904 40. 12 5. OE-30 AI399992. 1 EST HUMAN repetitive element ; 1. 93 4. OE-30 AW 937471. 1 EST HUMAN QV3-DT0043-090200-080-c06 DT0043 Homo sapiens cDNA 4. OE-30 AW 93471. 1 EST HUMAN QV3-DT0043-0920-080-c06 DT0043 Homo sapiens cDNA 1. 93 qq93c05. x1 Soares total fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE. 1938920 3'similar to 2082 7063 12172 2082 7063 12173, 3. 35 3. OE-30 AI338551. 1 EST HUMAN ins MER29. b2 MER29 repetitive element ; _ 3. OE-30 AF128893. 1 NT Homo sapiens telomerase ravarse transcriptase (TERT) gene, exons 1-6 1134 6139- 13680 3672 8677 Page 86 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORFSEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value 667 5692 10701 1.21 2.0E-30 AW857315.1 EST_HUMAN CM0-CT0307-310100-158-h03 CT0307 Homo sapiens cDNA 1068 6076 2. 46 2. 0E-30 F08688. 1 IEST HUMAN IHSC23F051 normallzed infant brain cDNA Homo sapiens cDNA clone c-23f05 1449 6446 11505 8. 55 2. 0E-30 Be175877.1 EST_HUMAN RC5-HT0582-110400-013-H08 HT0582 Homo sapiens cDNA 2643 7603 12716 6. 5 2. OE-30 BE765232. 1 EST HUMAN IL2-NT0101-280700-116-E04 NT0101 Homo sapiens cDNA 2849 7869 12885 6. 88 2. 0E-30 AF114156.1 NT Homo spaines Y-linked zinc finger protein (ZFY) gene, complete cds 3700 8704 13707 2. 23 2. OE-30 AW206581. 1 EST_HUMAN UI-H-BI1-afo-c-120-0-UI.s1 NCI_CGAP_Sub3 Homo sapiens cDNA clone IMAGE : 2722558 3 3' 4633 9618 14609 1. 61 2. 0E-30 BE298945. 1 EST HUMAN 601119860F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 3029438 5' 4633 9618 14610 1. 61 2. 0E-30 BE298945. 1 EST_HUMAN 601119860F1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE : 3029438 5' 284 5342 10355 13. 84 1. OE-30 C18939. 1 EST HUMAN C18939 Human placenta cDNA (TFujiwara) Homo sapiens cDNA clone GEN-570C01 5 ; id30b04. x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA done IMAGE : 2910991 3'similar to contains 533 5568 10571 3. 42 1. OE-30 AW468897. 1 EST HUMAN MER1. t3 MER1 MER1 repetitive element ; 706 5730 107463. 15 1. 0E-30 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 2150 7129 12246 2.78 1.0E-30 AA664377. 1 EST_HUMAN ac77b08.s1 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE : 868599 3' 2393 7364 12486 1.95 1.0E-30 BF347728. 1 EST_HUMAN 602022560F1 NCI_CGAP_Brn67 Homo sapiens cDNA clone IMAGE : 4157991 5' 2980 7998 13011 0.79 1.0E-30 AA315045.1 EST_HUMAN EST186868 HCC cell line (matastasis to liver in mouse) it Homo sapiens cDNA 5'end 1060 6069 11100 6. 71 8. 0E-31 8923389 NT Homo sapiens hypothetical protein FLJ20420 (FLJ20420), mRNA 2347 7321 23. 47 8. 0E-31 AL163208. 2 NT Homo sapiens chromosome 21 segment HS21 C008 4763 9747 14733 1.01 8. 0E-31 P23275 SWISSPROT OLFACTORY RECEPTOR 15 (OR3) 4763 9747 14734 1. 01 8. OE-31 P23275 SW ISSPROT OLFACTORY RECEPTOR 15 (OR3) 701 5725 2.43 7. 0E-31 AA372637. 1 EST HUMAN EST84555 Colon adenocarcinoma IV Homo sapiens cDNA 6'end 2597 7559 12675 2. 15 7. 0E-31 BE326517. 1 EST_HUMAN hw05a11.x1 NCI_CGAP_Lu24 Homo saplens cDNA clone IMAGE : 3182012 3' 2597 7559 12676 2. 15 7. 0E-31 BE326517. 1 EST HUMAN hw05a11. x1 NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3182012 3' Homo sapiens calcium channel alpha subunit (CACNA1E) gene, exons 7-49, and partial cds, alternativefy 3594 8601 2. 5 6. 0E-31 AF223391. 1 NT spliced 192 5256 10268 3.17 5.0E-31 M60694.1 NT Homo sapiens type I DNA toposiomerase gene, exon 8 192 5256 10269 3. 17 5. 0E-31 M60694. 1 NT Homo sapiens type) DNA topoisomerase gene, exon 8 591 $622 3. 18 4. 0E-31 AJ271735. 1 NT Homo sapiens Xq pseudoautosomat region ; segment 1/2 1782 6774 1. 4 4. OE-31 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21 C080 2716 7673 1.63 4. 0E-31 5730038 NT Homo sapiens SET domain and mariner transposase fusion gene (SETMAR) mRNA 2519 7486 12604 1. 54 3. 0E-31 6005871 NT Homo sapiens SEC63, endopiasmic reticulum translocon component (S. cerevisiae) like (SEC63L), mRNA 1873 6862 11951 2. 35 2. OE-31 AW838171. 1 EST_HUMAN QV2-LT0051-260300-111-f03 LT0051 Homo sapiens cDNA 2152 7131 12248 1. 01 2. OE-31 At393388. 1 EST_HUMAN tg44g05.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE;211672 3' 2278 7254 12373 1.52 2.0E-31 AL119245.1 EST_HUMAN DKFZp761G1513_r1 761 (synonym : hamy2) Homo sapiens cDNA clone DKFZp761G1513 5' Page 87 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Cells Probe Similar- ORF SEQ Expression (Top)Hit Top Hit Acession ID NO : Signal BLAST E No.Database Top Hit Descriptor NO: NO: Source Value aa88f11.s1 Stratagene fetal retina 937202 Homo saplens cDNA clone IMAGE:838413 3' similar to contains 2373 7345 12465 4. 03 2. 0E-31 AA458824. 1 EST HUMAN THR. t2 THR repetitive element ; Homo sapiens MAGE-B2 (MAGE-B2), MAGE-B3 (MAGE-B3), MAGE-B4 (MAGE-B4), and MAGE-B1 15 5095 10079 10. 14 1. OE-31 U93163. 1 NT (MAGE-B1) genes, complete cds 1623 6620 11686 9.05 1.0E-31 O95371 SWISSPROT OLFACTORY RECEPTOR 2C1 1623 6620 11687 9. 05 1. OE-31 095371 SWISSPROT OLFACTORY RECEPTOR 2C1 1623 6620 11688 9.05 1.0E-31 O95371 SWISSPROT OLFACTORY RECEPTOR 2C1 4501 9491 14467 1. 26 1.0E-31 AL134376. 1 EST HUMAN DKFZp547B235_r1 547 (synonym : hfbr1) Homo sapiens cDNA clone DKFZp547B235 5' 4501 9491 14468 1. 26 1.0E-31 AL134376. 1 EST_HUMAN DKFZp547B235_r1 547 (synonym : hfbr1) Homo sapiens cDNA clone DKFZp547B235 5' 2024 7007 12113 5.49 8.0E-32 AI056770. 1 EST_HUMAN oz15a09.x1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 1675384 3' 1017 6027 11057 84. 695. 0E-32 AF116627. 1 NT Homo sapiens PRO1181 mRNA, complete cds 918 5934 1. 7 4. OE-32 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21C046 453 5490 10505 2. 46 3.0E-32 Y17293. 1 NT Homo sapiens FLI-1 gene, partial 1427 6424 11483 57.22 3.0E-32 AV731500. 1 EST HUMAN AV731500 HTF Homo sapiens cDNA clone HTFAKC07 5' 2604 7566 1. 49 1. OE-32 D84430. 1 NT Homo sapiens mRNA for phenylalanyi tRNA synthetase, complete cds 3020 8037 1. 65 1. OE-32 BE743299. 1 EST HUMAN 601573207F1 NIH MGC 9 Homo sapiens cDNA clone IMAGE : 3834433 5' hw07c05. x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3182216 3'similar to TR : 088539 O88539 3400 8409 5. 22 9. OE-33 BE327112. 1 EST HUMAN WW DOMAIN BINDING PROTEIN 11. ; i 62 5142 10144 11. 74 7. OE-33 5031736 NT Homo sapiens short-chain alcohol dehydrogenase family member (HEP27) mRNA 62 5142 10145 11. 74 7. OE-33 5031736 NT Homo sapiens short-chain alcohol dehydrogenase family member (HEP27) mRNA to 12b09.x1 NCI_CGAP_Ut2 Homo sapiens cDNA clone IMAGE : 2178809 3'similar to contains OFR.t1OFR 2097 7078 12192 1.93 7.0E-33 AI590115. 1 EST HUMAN repetitive element ; 2576 7539 7. 47. 0E-33 AV730056. 1 EST HUMAN AV730056 HTF Homo sapiens cDNA clone HTFAVE06 5' 2757 6655 11727 1. 01 7. CE-33 AV730015. 1 EST_HUMAN AV730015 HTF Homo sapiens cDNA clone HTFANF08 5' 3168 8184 14. 85 7.0E-33 AW971307. 1 EST HUMAN EST383396 MAGE resequences, MAGL Homo sapiens cDNA 3652 8658 0. 8 6.0E-33 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21C085 1740 6735 1.43 5.0E-33 BF373515. 1 ESTHUMANQV1-FT0169-100700-271-a02 FT0169 Homo sapiens cDNA 1842 6832 0. 97 5. 0E-3 11141884 NT Homo sapiens solute carrier family 5 (choline transporter), member 7 (SLC5A7), mRNA 1858 6847 11934 4.15 5.0E-33 4507208 NT Homo sapiens spermidine synthase (SRM) mRNA 1858 6847 11935 4. 15 S. OE-33 4507208 NT Homo sapiens spermidine synthase (SRM) mRNA 2210 7187 1. 615. 0E-33 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21C085 3945 8843 13933 1. 87 5. OE-33 AB014599. 1 NT Homo sapiens mRNA for KIAA0699 protein, partial cds 5065 10034 15001 1. 075. 0E-33 M64350. 1 NT Human TCR variable region Va30 subfamily gene (VA30, JA, CA segments), 5'end 1111 6117 1.87 4.0E-33 AL163207. 2 NT Homo sapiens chromosome 21 segment HS21C007 Page 88 of 209 Table 4 sigle Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit ID NO: Signal BLAST E No. NO: NO: Source Value 2066 7048 12156 1.97 4.0E-33 4758987 NT Homo sapiens RAB1, member RAS oncogene family (RAB1) mRNA ab51 b11. r1 Stratagene lung carcinoma 937218 Homo sapiens cDNA clone IMAGE : 844317 5'similar to 2353 7327 1. 14 4. 0E-33 AA626621. 1 EST HUMAN contains Alu repetitive element ; contains MER28. b2 MER28 repetitive element ; 2474 7443 12558 2. 2 4. OE-33 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21 C010 4356 9347 14327 1. 46 4. 0E-33 AW293349. 1 EST_HUMAN UI-H-B12-ahl-c-03-0-UI.s1 NCI_CGAP_Sub4 Homo sapiens cDNA clone IMAGE : 2727149 3' ht09g01. x1 NCI_CGAP_Kid13 Homo sapiens cDNA clone IMAGE : 3146256 3'similar to contains MER29. b3 1073 6081 5. 08 3. OE-33 BE350127. 1 EST HUMAN MER29 repetitive element ; h<09g01. x1 NCtCGAPKid13 Homo sapiens cDNA clone IMAGE : 3146256 3'similar to contains MER29. b3 1074 6081 3. 92 3. 0E-33 BE350127. 1 EST HUMAN MER29 repetitive element ; 2382 7770 1. 73 3. 0E-33 AV647851. 1 EST HUMAN AV647851 GLC Homo sapiens cDNA clone GLCBCF09 3' qb67g03. x1 Soares_fetal heart_NbHH19W Homo sapiens cDNA clone IMAGE : 1705204 3'similar to 16 5096 1. 02 2. 0E-33 AI160189. 1 EST HUMAN contains OFR. t1 OFR repetitive element ; qb67g03. x1 Soares fetal heart NbHH19W Homo sapiens cDNA clone IMAGE : 1705204 3'similar to 105 5096 2. 37 2. 0E-33 AI160189. 1 EST HUMAN contains OFR. t1 OFR repetitive element ; 4295 9287 5. 1 2. OE-33 BE159039. 1 ESTHUMAN MRO-HT0405-160300-202-d08 HT0405 Homo sapiens cDNA ab51g11. r1 Stratagenelung carcinoma937218 HomosapienscDNA clone IMAGE : 844388 5'similar to 4826 9810 14791 30. 71 2. 0E-33 AA626683. 1 EST HUMAN gb : X00734_cds1 TUBULIN BETA-5 CHAIN (HUMAN) ; 4922 9900 14875 2. 2 2. OE-33 11421332 NT Homo sapiens hypothetical protein SIRP-b2 (SIRP-b2), mRNA 4922 9900 14876 2. 2 2. 0E-33 11421332 NT Homo sapiens hypothetical protein SIRP-b2 (SIRP-b2), mRNA Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat 9 5089 1. 61 1. OE-33 AF003528. 1 NT regions 4368 9360 14340 1. 09 8. 0E-34 BE062570. 1 ESTHUMAN QV2-BT0258-071299-019-g07 BT0258 Homo sapiens cDNA 1419 6416 11476 2. 31 7. 0E-34 T70845. 1 EST_HUMAN yd15e05.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 108320 5' 468 5504 10517 1. 48 6. 0E-34 U10991. 1 NT Human G2 protein mRNA, partial cds 468 5504 10518 1. 48 6. OE-34 U10991. 1 NT Human G2 protein mRNA, partial cds 1841 6831 2. 53 5. OE-34 7706500 NT Homo sapiens Npw38-binding protein NpwBP (LOC51729), mRNA 4893 9872 14839 5.04 5.0E-34 U30883. 1 NT Human splicing factor SRp55-1 (SRp-55) mRNA, complete cds 1952 6938 12040 1. 36 4. 0E-34 Al804667. 1 EST_HUMAN tt94c06.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE : 2249194 3' 2647 7607 12719 0. 92 4. 0E-34 8922807 NT Homo sapiens hypothetical protein FLJ10989 (FLJ10989), mRNA 3099 8115 13133 1.38 4. 0E-34 5803166 NT Homo sapiens splicing factor 3a, subunit 3, 60kD (SF3A3), mRNA ADP, ATP CARRIER PROTEIN, LIVER ISOFORM T2 (ADP/ATP TRANSLOCASE 3) (ADENIN 1475 6472 11530 14. 56 1. OE-34 P12236 SW ISSPROT NUCLEOTIDE TRANSLOCATOR 3) (ANT 3) Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat 3589 8596 13600 1. 32 1. OE-34 AF003528. 1 NT regions Page 89 of 209 Table 4 Sinale Exon Probes Exnressed in HBL100 Cells Probe Similar- ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: NO: Source Value 3957 8955 13944 0.93 1.0E-34 AY009397.1 NT Homo sapiens WNT3 precursor (WNT3) mRNA, complete cds 3957 8955 13945 0. 93 1. OE-34 AY009397. 1 NT Homo sapiens WNT3 precursor (WNT3) mRNA, complete cds 4352 9343 4. 5 1. 0E-34 BE071414. 1 EST_HUMAN RC2-BT0505-240400-016-h08 BT0506 Homo sapiens cDNA 3560 8567 13573 1. 41 9. OE-35 AW663302. 1 EST HUMAN hh77b06. y1 NCI CGAP GU1 Homo sapiens cDNA clone IMAGE : 2968787 5' 224 5286 25. 01 8. OE-35 6031190 NT Homo sapiens prohibitin (PHB) mRNA naa33a08.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 3258134 3'similar to TR : 075912 1699 6694 11770 2. 3 8. OE-35 BF589937. 1 EST HUMAN 075912 DIACYLGLYCEROL KINASE IOTA. ; naa33a08. x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 3258134 3'similar to TR : 075912 1699 6694 11771 2. 3 8.0E-35 BF589937. 1 EST HUMAN 075912 DIACYLGLYCEROL KINASE IOTA. ; 4717 9702 14688 3. 04 8. 0E-35 BF183195. 1 EST_HUMAN 601809588F1 NIH_MGC_18 Homo sapiens cDNA clone IMAGE : 4040324 5' 1388 6385 11437 1. 5 6. 0E-35 AA757115. 1 EST HUMAN ah53h03. s1 Soares testis NHT Homo sapiens cDNA clone 1309397 3' 1925 6911 12006 1. 65 6. OE-35 6005975 NT Homo sapiens zinc finger protein 208 (ZNF208), mRNA 5063 10032 0. 75 6. 0E-35 8923389 NT Homo sapiens hypothetical protein FLJ20420 (FLJ20420), mRNA 1671 6667 11742 1. 53 5. OE-35 X63392. 1 NT H. sapiens immunoglobulin kappa light chain variable region L14 2711 7668 12781 1. 05 5. 0E-35 AB007866. 2 NT Homo sapiens mRNA for KIAA0406 protein, partial cds 2939 7959 12977 1. 22 5. OE-35 6912639 NT Homo sapiens Ring1 and YY1 binding protein (RYBP), mRNA Homo sapiens clk2 kinase (CLK2), propin1, cote1, glucocerebrosidase (GBA), and metaxin genes, complete cds ; metaxin pseudogene and glucocerebrosidase pseudogene ; and thrombospondin3 (THBS3) gene, partial 4285 9277 14265 1. 3 5.0E-35 AF023268. 1 NT cds 1408 6406 11465 66. 29 4. OE-35 BE257907. 1 EST HUMAN 601109719F1 NIH MGC 16 Homo sapiens cDNA clone IMAGE : 3350405 5' yu98a07. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 241236 5'similar to contains 1783 6775 11866 19. 99 4. 0E-35 H91193. 1 EST HUMAN PTR5 repetitive element ; 1544 6542 11600 52.74 3. OE-35 BE268182. 1 EST_HUMAN 601125260F1 NIH_MGC_8 Homo sapiens cDNA clone IMAGE : 3345063 5' 2269 7246 1. 68 3. 0E-35 AF224492. 1 NT Homo sapiens phospholipid scramblase 1 gene, complete cds K6932F Human fetal heart, Lambda ZAP Express Homo sapiens cDNA clone K6932 5'similar to 108 7713 10192 1. 21 2. 0E-35 N88965. 1 EST_HUMAN REPETITIVE ELEMENT 1168 6171 11206 1. 09 2. 0E-35 T11909. 1 EST_HUMAN A971 F Heart Homo sapiens cDNA clone A971 2156 7135 12254 2. 74 2. 0E-35 AB018413. 1 NT Homo sapiens mRNA for Gab2, complete cds hi86a12. x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2979166 3'similar to 2612 7574 12688 1.92 2. 0E-35 AW665005. 1 EST HUMAN SW : TR12 HUMAN Q14669 THYROID RECEPTOR INTERACTING PROTEIN 12 ; 3480 8488 0. 84 2. 0E-35 AB020702. 1 NT Homo sapiens mRNA for KIAA0895 protein, partial cds TCBAP2E4328 Pediatric pre-B cell acute lymphoblastic leukemia Baylor-HGSC project=TCBA Homo sapiens 3814 8817 13823 0. 81 2. 0E-35 BE247575. 1 EST_HUMAN cDNA clone TCBAP4328 Page 90 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar set SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. source NO: NO: Source Value TCBAP2E4328 Pediatric pre-B cell acute lymphoblastic leukemia Baylor-HGSC project-TCBA Homo sapiens 3814 8817 13824 0. 81 2. OE-35 BE247575. 1 EST HUMAN cDNA clone TCBAP4328 4535 9525 2. 63 2. 0E-35 H49239.1 EST_HUMAN yq19a12.r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 274079 5' 47 5128 10119 6. 23 1. 0E-35 AA631949. 1 EST_HUMAN fmfc16 Regional genomic DNA specific cDNA library Homo sapiens cDNA clone CR12-1 47 5128 10120 6. 23 1. OE-35 AA631949. 1 EST HUMAN fmfc16 Regional genomic DNA specific cDNA library Homo sapiens cDNA clone CR12-1 743 5766 10790 140. 65 1. OE-35 AW389473. 1 EST_HUMAN IL2-ST0162-131099-006-d12 ST0162 Homo sapiens cDNA 743 5766 10791 140. 65 1. 0E-35 AW389473. 1 EST HUMAN IL2-ST0162-131099-006-d12 ST0162 Homo sapiens cDNA yd93a01. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 115752 5'similar to 898 5916 1. 2 1. OE-35 T87947. 1 EST HUMAN SP : A44282 A44282 RETROVIRUS-RELATED POL POLYPROTEIN-HUMAN ; 2471 7439 12555 2. 17 1. 0E-35 7705994 NT Homo sapiens hypothetical protein (LOC51233), mRNA ht09g01. x1 NCI_CGAP_Kid13 Homo sapiens cDNA clone IMAGE : 3146256 3'similar to contains MER29. b3 2694 7652 12766 1. 37 1. OE-35 BE350127. 1 EST HUMAN MER29 repetitive element ; ht09g01.x1 NCI_CGAP_Kid13 Homo sapiens cDNA clone IMAGE : 3146256 3'similar to contains MER29. b3 2694 7652 12767 1. 37 1. 0E-35 BE350127. 1 EST HUMAN MER29 repetitive element ; 3070 8086 13100 1. 24 1. OE-35 6006030 NT Homo sapiens transcription elongation factor B (SIII), polypepfide 1-like (TCEB1L) mRNA 3090 8106 13121 2. 49 1. 0E-35 AV650422. 1 EST HUMAN AV650422 GLC Homo sapiens cDNA clone GLCCEF06 3' 3090 8106 13122 2. 49 1. 0E-35 AV650422. 1 EST HUMAN AV650422 GLC Homo sapiens cDNA clone GLCCEF06 3' 4298 9290 14276 4. 67 1. OE-35 7656905 NT Mus musculus activin receptor interacting protein 1 (Arip1-pending), mRNA 4298 9290 14277 4. 67 1. OE-35 7656905 NT Mus musculus activin receptor interacting protein 1 (Arip1-pending), mRNA 3879 8880 13883 1. 75 9. 0E-36 AW821707. 1 EST HUMAN RC3-ST0315-180200-013-f12 ST0315 Homo sapiens cDNA 2860 7880 12898 2. 3 7. OE-36 AW857579. 1 ESTHUMAN CM1-CT0315-091299-063-d07 CT0315 Homo sapiens cDNA 3044 8061 4. 78 7. 0E-36 4557498 NT Homo sapiens C-terminal binding protein 2 (CTBP2) mRNA 1957 6943 12045 1. 89 6. 0E-36 7706622 NT Homo sapiens ninjurin 2 (NINJ2), mRNA 2351 7325 6. 02 6. 0E-36 AB035346. 1 NT Homo sapiens TCL6 gene, exon 12 3554 8561 13567 1. 16 6. 0E-36 BF515101. 1 EST_HUMAN UI-H-BW1-anv-c-12-0-UI.s1 NCI_CGAP_Sub7 Homo sapiens cDNA clone IMAGE : 3083542 3' 138 5204 10219 10. 77 5. 0E-36 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1/2 2678 7636 12751 24. 76 5. 0E-36 BE388436. 1 EST_HUMAN 601285567F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3607289 5' 3531 8537 13542 1.37 5.0E-36 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21 C009 4643 9628 14621 2. 22 5. OE-36 5729729 NT Homo sapiens AP15-like 1 (API5L1), mRNA 4643 9628 14622 2. 22 5. OE-36 5729729 NT Homo sapiens API5-like 1 (API5L1), mRNA 1205 6206 11243 2. 05 4. 0E-36 BE010038.1 EST_HUMAN PM3-BN0176-100400-001-g04 BN0176 Homo sapiens cDNA RETROVIRUS-RELATED POL POLYPROTEIN [CONTAINS : REVERSE TRANSCRIPTASE ; 1416 6413 1147 1. 38 4. 0E-36 P10266 SW ISSPROT ENDONUCLEASE] 1606 6602 11664 1. 78 4. 0E-36 BE382574. 1 EST_HUMAN 601298574F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 3628386 5' Page 91 of 209 Tabie 4 Single Exon Probes Fcpressed in HBL100 Cells Probe Exon MostSimilar TopHit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor NO: NO: ID NO: Signal BLAST E No. source Value 2161 7140 4. 99 4. OE-36 AW247772. 1 EST HUMAN 2820020. Sprime NIH_MGC_7 Homo sapiens cDNA clone IMAGE : 2820020 5' 3275 8287 13311 0.98 4.0E-36 BE389299. 1 EST_HUMAN 601282266F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3604168 5' 3275 8287 13312 0.98 4.0E-36 BE389299. 1 EST_HUMAN 601282266F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3604168 5' 687 5711 10725 2.91 3.0E-36 AF099810. 1 NT Homo sapiens neurexin III-alpha gene, partial cds 1468 6465 11524 1. 33. 0E-36 AF110239. 1 NT Homo sapiens calcium/calmodulin-stimulated cyclic nucleotide phosphodiesterase (PDE1A) gene, partial cds 1468 6465 11525 1. 3 3.0E-36 AF110239. 1 NT Homo sapiens calciumicalmodulin-stmulated cyclic eotidephosphodiesterase (PDE1A) gene, partial cds 2235 7212 12329 2. 14 3.0E-36 7662401 NT Homo sapiens KIAA0952 protein (KIAA0952), mRNA 4373 9365 14345 6.39 3.0E-36 10181139 NT Mus musculus junctophilin 1 (Jp1-pending), mRNA 3096 8112 13130 6. 65 2. OE-36 BE259267. 1 EST HUMAN 601106343F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE : 3342706 5' 4802 9786 14768 17. 96 2.0E-36 AW880376. 1 EST HUMAN QVO-OT0030-240300-174-h04 OT0030 Homo sapiens cDNA 874 5892 10933 1. 87 1. OE-36 BE409310. 1 EST HUMAN 601300938F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3635480 5' 2084 7065 12176 1.85 1.0E-36 BE146523. 1 EST_HUMAN RC1-HT0217-131199-021-hO7 HT0217 Homo sapiens cDNA 2084 7065 12177 1. 85 1. 0E-36 BE146523. 1 EST HUMAN RC1-HT0217-131199-021-h07 HT0217 Homo sapiens cDNA 2139 7119 12234 1.5 1.0E-36 BF673761. 1 EST_HUMAN 602136493F1 NIH_MGC_83 Homo sapiens cDNA clone IMAGE:4272886 5' 3269 8282 1. 42 1. OE-36 AF156962. 1 NT Homo sapiens human endogenous retrovirus W proC6-19 protease (pro) gene, partial cds 3276 8288 13313 0. 98 8. OE-37 4757979 NT Homo sapiens chimerin (chimaerin) 2 (GHN2) mRNA 1265 6263 2. 66 7. OE-37 AL042800. 1 EST_HUMAN DKFZp434E0422_r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434E0422 5' 1705 6700 11776 1.1 7.0E-37 AF111167. 2 NT Homo sapiens jun dimerization protein gene, partial cds ; cfos gene, complete cds; and unknown gene 1705 6700 11777 1. 1 7.0E-37 AF111167. 2 NT Homo sapiens jun dimerization protein gene, partial cds ; cfos gene, complete cds ; and unknown gene 4955 9932 1. 57 6. OE-37 R10039. 1 EST HUMAN yf25a02. r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 127850 5' 2357 7331 12447 2.14 4.0E-37 AA702794.1 EST_HUMAN zi90b04.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 448015 3' 5038 10009 0. 91 4. OE-37 N62051. 1 EST HUMAN EST52g10 WATM1 Homo sapiens cDNA clone 52g10 similar to human STS G04101 1967 6952 12056 1. 95 3.0E-37 AL048956.1 EST_HUMAN DKFZp434L2418_r1 434 (synonym: htes3) Homo sapiens cDNA clone DKFZp434L2418 1967 6952 12057 1. 95 3. OE-37 AL048956. 1 EST HUMAN DKFZp434L2418 r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434L2418 2441 7441 1.2 3.0E-37 AW961150.1 EST_HUMAN EST373222 MAGE resequences, MAGF Homo sapiens cDNA 2895 7914 3. 82 3. OE-37 AW961150. 1 EST HUMAN EST373222 MAGE resequences, MAGF Homo sapiens cDNA 4831 9815 0.76 3.0E-37 BF035327. 1 EST_HUMAN 601458531F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3862086 5' 380 5455 10472 1. 71 2. OE-37 D89790. 1 NT Homo sapiens mRNA for AML1, complete cds 380 5455 10473 1. 71 2. OE-37 D89790. 1 NT Homo sapiens mRNA for AML1, complete cds 1064 6072 11104 2. 16 2.0E-37 AU131202. 1 EST_HUMAN AU131202 NT2RP3 Homo sapiens cDNA clone NT2RP3002166 5' Page 92 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value 1064 6072 11105 2.16 2.0E-37 AU131202.1 EST_HUMAN AU131202 NT2RP3 Homo sapiens cDNA clone NT2RP3002166 5' 19236909 120041. 452. 0E-37 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21 C047 Homo sapiens cytochrome P450, subfamily XXVIIA (steroid 27-hydroxylase, cerebrotendinous 3798 8801 13806 4. 94 2. OE-37 4503210 NT xanthomatosis), polypeptide 1 (CYP27A1b) mRNA 4123 9118 14104 0. 78 2. OE-37 4826685 NT Homo sapiens DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1 (DDX1) mRNA 2034 7017 12127 3. 59 1.0E-37 AL163281. 2 NT Homo sapiens chromosome 21 segment HS21 C081 3124 8140 0. 98 1. OE-37 AW862082. 1 EST_HUMAN RC3-CT0347-210400-016-h03 CT0347 Homo sapiens cDNA 3855 8857 13863 1.18 1.0E-37 AF189011. 1 NT Homo sapiens ribonuclease III (RN3) mRNA, complete cds 4783 9767 14751 2. 02 1. OE-37 BF371719. 1 EST_HUMAN QV0-FN0180-280700-318-c10 FN0180 Homo sapiens cDNA 1202 6203 11240 1. 69 8. OE-38 11436955 NT Homo sapiens Grb2-associated binder 2 (KIAA0571), mRNA 2425 7396 12517 1.23 8.0E-38 BF346221. 1 EST HUMAN 602018401 F1 NC) CGAPBrn67 Homo sapiens cDNA done) IMAGE : 4153992 5' 2119 7099 12212 5. 28 7. OE-38 AW972825. 1 EST HUMAN EST384920 MAGE resequences, MAGL Homo sapiens cDNA 2969 7987 13001 2. 99 6. OE-38 BF033033. 1 EST_HUMAN 601455722F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3859348 5' 717 5740 10757 1. 86 5. OE-38 AW971819. 1 EST HUMAN EST383908 MAGE resequences, MAGL Homo sapiens cDNA 2385 7356 12478 4. 11 5. OE-38 AJ237740. 1 NT Homo sapiens BIBIIR gene (partial), exon 8 4991 7356 12478 1. 09 5.0E-38 AJ237740. 1 NT Homo sapiens RIBIIR gene (partial), exon 8 119 5189 10200 3. 97 4. 0E-38 Z25466. 1 NT B. taurus mitochondrial aspartate aminotransferase mRNA, complete CDS 119 5189 10201 3.97 4.0E-38 Z25466. 1 NT B. taurus mitochondrial aspartate aminotransferase mRNA, complete CDS 2043 7025 2.4 3.0E-38 AF003530. 1 NT Homo sapiens homeobox protein CDX4 (CDX4) gene, complete cds and flanking repeat regions 3613 8620 1. 58 3. OE-38 7549807 NT Homo sapiens HIRA interacting protein 4 (dnaJ-like) (HIRIP4), mRNA 3765 8768 13772 1. 58 3. OE-38 P53538 SW ISSPROT SSU72 PROTEIN 3765 8768 13773 1. 58 3. OE-38 P53538 SWISSPROT SSU72 PROTEIN 4482 9472 1. 26 3. OE-38 BE279301. 1 EST HUMAN 601157633F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3504272 5' 51 5132 10127 1. 71 2. OE-38 AL163248. 2 NT Homo sapiens chromosome 21 segment HS21C048 13616358 11408 8. 04 2. OE-38 5902097 NT Homo sapiens SMT3 (suppressor of mif two 3, yeast) homolog 2 (SMT3H2), mRNA zs30d01.r1 Soares ovary tumor NbHOT Homo sapiens cDNA clone IMAGE : 770785 5'similar to 1607 6603 11665 1.7 2.0E-38 AA437353. 1 EST HUMAN SW ;MA12_RABIT P45701 MANNOSYL-OLIGOSACCHARIDE ALPHA-1,2-MANNOSIDASE; zw30d01. r1 Scares ovary tumor NbHOT Homo sapiens cDNA clone IMAGE : 770785 5'similar to 1607 6603 11666 1.7 2.0E-38 AA437353. 1 EST HUMAN SW : MA12 RABIT P45701 MANNOSYL-OLIGOSACCHARIDE ALPHA-1, 2-MANNOSIDASE ; 3455 8463 0.91 2.0E-38 AF070670. 1 NT Homo sapiens protein phosphatase 2C alpha 2 mRNA, complete cds 4448 943 14421 5 2. 0E-38 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA 4953 9930 14909 0.75 2.0E-38 BE296224. 1 EST HUMAN 601177386F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 3532580 5' 4953 9930 14910 0. 75 2.0E-38 BE296224. 1 EST HUMAN 601177386F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 3532580 5' Page 93 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Top Hit Descriptor Value zu62b02. r1 SoarestestisNHT Homo sapiens cDNA clone IMAGE : 742539 5'similar to contains element 1076 6083 1. 97 1. OE-38 AA401570. 1 EST HUMAN MER19 repeti6ve element ; 1953 6939 12041 3. 28 1. 0E-38 4885288 NT Homo sapiens guanine nucleotide binding protein-like 1 (GNL1), mRNA 1970 6955 12059 0. 96 1. 0E-38 7661969 NT Homo sapiens KIAA0173 gene product (KIAA0173), mRNA 2423 7394 12515 2.9 1.0E-38 AF270831. 1 NT Homo sapiens cyclin K (CCNK) gene, exon 7 4192 9185 14166 0. 72 1. 0E-38 4505016 NT Homo sapiens low density lipoprotein receptor-related protein 6 (LRP6) mRNA, and translated products 4197 9190 14171 1.27 1.0E-38 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21C003 4197 9190 14172 1.27 1.0E-38 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 4466 9456 144361. 06 1. 0E-388922543 NT Homo sapiens hypothetical protein FLJ10600 (FLJ10600), mRNA 55 5136 10133 6. 42 8. 0E-39 4502312 NT Homo sapiens ATPase, H+ transporting, lysosomal (vacuolar proton pump) 16kD (ATP6C) mRNA 1371 6368 11417 1. 13 8. OE-39 4758229 NT Homo sapiens estrogen receptor-binding fragment-associated gene 9 (EBAG9) mRNA wh53f10. x1 NCI CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2384491 3'similar to TR : P87890 P8789 ( 1793 6784 1. 43 8. OE-39 AI823404. 1 EST HUMAN POL PROTEIN ; 2038 7021 12130 4. 22 7. 0E-39 AL163227. 2 NT Homo sapiens chromosome 21 segment HS21 C027 Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat 992 6005 11036 1. 9 S. OE-39 AF003528. 1 NT regions at36b04. x1 Barstead colon HPLRB7 Homo sapiens cDNA clone IMAGE : 2374063 3'similar toTR : Q15408 2916 7935 12953 6. 76 5. OE-39 AI750154. 1 EST HUMAN Q15408 NEUTRAL PROTEASE LARGE SUBUNIT ; contains LTR7. t1 LTR7 repetitive element ; 546 5580 10584 50. 63 4. OE-39 AB015610. 1 NT Chlorocebus aethiops mRNA for ribosomal protein S4X, complete cds 3492 8500 13514 0. 7 4. OE-39 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 48 5129 10121 18. 3 3. 0E-39 AA631949.1 EST_HUMAN fmfc16 Regional genomic DNA specific cDNA library Homo sapiens cDNA clone CR12-1 48 5129 10122 18.3 3.0E-39 AA631949.1 EST_HUMAN fmfo16 Regional genomic DNA specifio cDNA library Homo sapiena cDNA clone CR12-1 48 5129 10123 18. 3 3.0E-39 AA631494. 1 ESTHUMAN fmfc16 Regional genomic DNA specific cDNA library Homo sapiens cDNA clone CR12-1 886 5904 18. 94 2. 0E-39 BE409203. 1 EST HUMAN 601301607F1 NIH_MGC 21 Homo sapiens cDNA clone IMAGE : 3636289 5' 901 5919 8. 24 2. 0E-39 AI525119. 1 EST HUMAN promrna-7. D01. r bvtumor Homo sapiens cDNA 5' 1015 6025 3. 1 2. OE-39 AF000573. 1 NT Homo sapiens homogentisate 1, 2-dioxygenase gene, complete cds 1498 6495 89. 79 AW372318. OE-39 EST_HUMAN PM0-BT0340-211290-003-d02 BT0340 EST-HUMAN PMO-BT0340-211299-003-dO2 cDNA nw21g02.s1 NCI_CGAP_GCB0 Homo sapiens cDNA clone IMAGE : 1241138 3'similar to contains THR. t3 1929 6915 12012 2.58 2. 0E-39 AA720574. 1 EST HUMAN THR repetitive element ; 2556 7521 12638 1. 41 2. 0E-39 AL163248. 2 NT Homo sapiens chromosome 21 segment HS21 C048 4279 9272 14261 1. 83 2.0E-39 BF370207. 1 EST_HUMAN RC4-FN0037-290700-011-a10 FN0037 Homo sapiens cDNA 1482 6479 11535 11.08 1.0E-39 AJ006345. 1 NT Homo sapiens KVLQT1 gene Page 94 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. Source Value 1482 6479 11536 11. 08 1. OE-39 AJ006345. 1 NT Homo sapiens KVLQT1 gene 1499 6497 11550 4. 37 1. OE-39 7657020 NT Homo sapiens DKFZp434P211 protein (DKFZP434P211), mRNA 4525 9515 14500 15. 01 1.0E-39 AW951995. 1 EST_HUMAN EST364065 MAGE resequences, MAGB Homo sapiens cDNA 4525 9515 14501 15. 01 1. 0E-39 AW951995. 1 EST HUMAN EST364065 MAGE resequences, MAGB Homo sapiens cDNA 4564 9552 14538 7. 93 1. OE-39 7657020 NT Homo sapiens DKFZp434P211 protein (DKFZP434P211), mRNA 551 5585 10587 1. 74 9. OE-40 5803210 NT Homo sapiens UDP-glucose pyrophosphorylase 2 (UGP2), mRNA 1215 6214 11253 11. 93 9. 0E-40 4755145 NT Homo sapiens AE-binding protein 1 (AEBP1) mRNA 1215 6214 11254 11. 93 9. OE-40 4755145 NT Homo sapiens AE-binding protein 1 (AEBP1) mRNA Homo sapiens tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory) (TIMP3 ; 1423 6420 11480 1. 06 9. 0E-40 4507512 NT mRNA 3697 8701 13704 1. 19 9. OE-40 4503764 NT Homo sapiens fragile X mental retardation 1 (FMR1) mRNA 3866 10048 13870 3. 4 9. 0E-40 AB033070. 1 NT Homo sapiens mRNA for KIAA1244 protein, partial cds 4370 9214 14193 0. 88 9. 0E-40 4507848 NT Homo sapiens ubiquitin specific protease 13 (isopeptidase T-3) (USP13) mRNA 2968 7986 13000 0. 96 8. 0E-40 AA078165. 1 EST HUMAN 7H15A04 Chromosome 7 HeLa cDNA Library Homo sapiens cDNA clone 7H15A04 3823 8825 4. 61 8. 0E-40 BE396541. 1 EST HUMAN 601288958F1 NIH MGC 8 Homo sapiens cDNA clone IMAGE : 3619166 5' 2654 7614 12724 6. 7 6. 0E-40 AA361275. 1 EST HUMAN EST70527 T-cell lymphom Homo sapiens cDNA 5'end similar to similar to zinc finger protein family 2654 7614 12725 6. 7 6. 0E-40 AA361275. 1 EST HUMAN EST70527 T-cell lymphom Homo sapiens cDNA 5'end similar to similar to zinc finger protein family 2529 7495 12615 1.57 5. 0E-40 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 tt91 b01. x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE : 2248873 3'similar to TR : 073505 073505 1838 6828 11917 1. 77 4. 0E-40 686005. 1 EST_HUMAN. POL PROTEIN.; Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat 2050 7032 2. 06 4. 0E-40 AF003528. 1 NT regions 4264 9257 14247 8. 89 4. OE-40 7662117 NT Homo sapiens KIAA0433 protein (KIAA0433), mRNA 4011 9007 13996 0. 99, 3. 0E-40 AI925949. 1 EST HUMAN wh12f07. x1 NCI CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2380549 3' 323 5377 3.68 2.0E-40 AI223036.1 EST_HUMAN qq52h08.x1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE:1838847 3' xr24e10.x1 NCI_CGAP_Ut4 Homo sapiens cDNA clone IMAGE:2761098 3' similar to SW:RS5_MOUSE 786 5807 47. 86 2. 0E-40 AW303868. 1 EST HUMAIN P97461 40S RIBOSOMAL PROTEIN S5. ; 1790 6781 2. 37 2. 0E-40 AV731601. 1 EST HUMAN AV731601 HTF Homo sapiens cDNA clone HTFAZE05 5' Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 7 (PSMA7) mRNA, and translated 1894 6882 11973 6. 41 2. 0E-40 4505188 NT products Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 7 (PSMA7) mRNA, and translated 1894 6882 11974 6. 41 2. 0E-40 4506188 NT products Page 95 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Probe Exon Most Similar ORS SET Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source NO: NO: Source Value 2105 7085 12200 1.63 2.0E-40 5453592 NT Homo sapiens adenylyl cyclase-associated protein 2 (CAP2) mRNA 2618 7580 1. 3 2. 0E-40 BE275932. 1 EST HUMAN 601121567F1 NIH_MGC 20 Homo sapiens cDNA done) IMAGE : 3345784 5' 3053 8070 13080 4. 08 2. OE-40 5453592 NT Homo sapiens adenylyl cyclase-associated protein 2 (CAP2) mRNA 4745 9730 14717 1. 8 2.0E-40 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21C080 4745 9730 14718 1. 8 2. OE-40 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21C080 5005 9976 14951 1 2. OE-40 4505880 NT Homo sapiens plasminogen (PLG) mRNA 872 5890 1. 42 1. 0E-40 AA225989. 1 EST_HUMAN nc09a09.s1 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE : 1007608 2548 7513 12631 1. 42 1. OE-40 BF036881. 1 EST_HUMAN 601460375F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3863803 5' bb79a10.y1 NIH_MGC_10 Homo sapiens cDNA clone IMAGE : 3048570 5'similar to TR : Q9Z158 Q9Z158 2614 7576 1. 6 1. OE-40 BE018348. 1 EST HUMAN SYNTAXIN 17. ; 2663 7622 12733 0. 99 1. 0E-40 BF541030. 1 EST_HUMAN 602068604F1 NIH_MGC_58 Homo sapiens cDNA done) IMAGE : 4067736 5' 2663 7622 12734 0. 99 1. 0E-40 BF541030. 1 EST_HUMAN 602068604F1 NIH_MGC_58 Homo sapiens cDNA clone IMAGE : 4067736 5' 3224 8239 1. 22 1. 0E-40 4507142 NT Homo sapiens sorting nexin 3 (SNX3) mRNA 4478 9468 14448 5. 47 1. OE-40 4508012 NT Homo sapiens zinc finger protein 200 (ZNF200) mRNA, and translated products 3715 8719 13720 1. 02 9. OE-41 W01596. 1 EST_HUMAN za36aO2. r1 Soares fetal liver spleen 1 N FLS Homo sapiens cDNA clone IMAGE : 294602 5' 818 7732 10874 1. 8 7. 0E-41 AI934364. 1 EST_HUMAN wp04h04.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2463895 3' 818 7732 10875 1. 8 7. 0E-41 AI934364. 1 EST_HUMAN wp04h04.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2463895 3' 5041 10012 14981 1. 23 7. 0E-41 11431114 NT Homo sapiens hypothetical protein (FLJ10996), mRNA 278 5336 10350 2. 15 6.0E-41 AB037163. 1 NT Homo sapiens DSCR5b mRNA, complete cds 2052 7034 12146 4. 09 6. OE-41 7657042 NT Homo sapiens Down syndrome candidate region 1 (DSCR1), mRNA 1766 6758 11844 1. 57 5. 0E-41 T62628. 1 ESTHUMAN yc03e10. s1 Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE : 79626 3' 390 5428 1. 45 4. 0E-41 BE156318. 1 EST-HUMAN 1081 6088 11117 1. 03 4. 0E-41 AU119344. 1 EST HUMAN AU119344 HEMBA1 Homo sapiens cDNA clone HEMBA1005583 5' ow45e06. s1 SoaresparathyroidtumorNbHPA Homo sapiens cDNA clone IMAGE : 1649794 3'similar to 1385 6382 11432 10. 1 4. 0E-41 AI027117. 1 EST HUMAN TR : 000597 000597 CYTOCHROME C-LIKE POLYPEPTIDE. contains LTR5. b1 LTR5 repetitive element ; ow45e06. s1 SoaresparathyroidtumorNbHPA Homo sapiens cDNA clone IMAGE : 1649794 3'similar to 1385 6382 11433 10. 1 4. 0E-41 AI027117. 1 EST HUMAN TR : 000597 000597 CYTOCHROME C-LIKE POLYPEPTIDE. ; contains LTR5. b1 LTR5 repetitive element ; 1398 6395 11450 3. 73 4. 0E-41 AB008681. 1 NT Homo sapiens gene for activin receptor type IIB, complete cds tm96c04. x1 NC) CGAPBrn25 Homo sapiens cDNA clone IMAGE : 2165958 3'similar to contains OFR. b1 1595 6591 11652 4. 42 4. 0E-41 AI500406. 1 EST HUMAN OFR repetitive element ; 2818 7838 12854 3. 89 4. 0E-41 AJ229041. 1 NT Homo sapiens 959 kb contig between AML1 and CBR1 on chromosome 21q22 ; segment 1/3 2818 7838 12855 3. 89 4. 0E-41 AJ229041. 1 NT Homo sapiens 959 kb contig between AML1 and CBR1 on chromosome 21q22 ; segment 1/3 Page 96 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Cells » Most Similar ORS SET Expression (Top) Hit Top Hit Acession Top HIt SEQ ID SEQ ID ID NO: Signal BALST E No. Source NO: NO: Source Value 4021 9017 14004 2.21 4.0E-41 X92685.1 NT H.sapiens DNase I hypersensitive site (HSS-3) enhancer element 933 5950 10983 1. 82 3. OE-41 AB030176. 1 NT Homo sapiens PAD-H19 mRNA for peptidylarginine deiminase type II, complete cds Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 1/2 (DLEC1, ORCTL3, ORCTL4 genes, 4208 9201 14183 3.05 3.0E-41 AB026898. 1 NT complete cds) 1789 6527 11586 49. 39 2. OE-41 U43701. 1 NT Human ribosomal protein L23a mRNA, complete cds 1918 6904 11998 1. 61 2. OE-41 AA331940. 1 EST HUMAN EST35818 Embryo, 8 week I Homo sapiens cDNA 5'end 2157 7136 12255 5. 84 2. OE-41 D86962. 1 NT Human mRNA for KIAA0207 gene, complete cds 2206 7183 12306 15. 51 2.0E-41 X89631.1 NT G.gorilla DNA for ZNF80 gene homolog 2755 6527 11586 16. 52 2. OE-41 U43701. 1 NT Human ribosomal protein L23a mRNA, complete cds 3748 8752 13751 0.8 2.0E-41 5032106 NT Homo sapiens son of seveniess (Drosophila) homolog 1 (SOS1) mRNA 4488 9478 14458 1.15 2.0E-41 AL163267. 2 NT Homo sapiens chromosome 21 segment HS21 C067 4488 9478 14459 1.15 2.0E-41 AL163267. 2 NT Homo sapiens chromosome 21 segment HS21 C067 3133 8149 13170 1. 16 1.0E-41 BE869735. 1 EST HUMAN 601445647F1 NIH MGC 65 Homo sapiens cDNA clone IMAGE : 3849803 5' 3133 8149 13171 1.16 1.0E-41 BE869735. 1 EST_HUMAN 601445647F1 NIH_MGC_65 Homo sapiens cDNA clone IMAGE : 3849803 5' 4434 9424 14409 15.19 1.0E-41 6678468 NT Mus musculus tubulin alpha 6 (Tuba6), mRNA 460 5497 10508 5. 2 8.0E-42 AF003530. 1 NT Homo sapiens homeobox protein CDX4 (CDX4) gene, complete cds and flanking repeat regions 4976 9952 14930 0. 94 8. OE-42 6679031 NT Mus musculus neural precursor cell expressed, developmentally down-regulated gene 1 (Nedd1), mRNA 919 5935 1.72 7.0E-42 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21C085 1819 6809 11900 3.13 6.0E-42 AF012872. 1 NT Homo sapiens phosphatidylinositol 4-kinase 230 (pi4K230) mRNA, complete cds 1819 6809 11901 3. 13 6. OE-42 AF012872. 1 NT Homo sapiens phosphatidylinositol 4-kinase 230 (pi4K230) mRNA, complete cds xp29f08.x1 NCI_CGAP_HN10 Homo sapiens cDNA clone IMAGE : 2741799 3'similar to contains L1. t1 L1 2227 7204 2. 79 6. 0E-4= AW238656. 1 ESTHUMAN repetitive eiement ; 136 5202 5. 47 5. OE-42 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1/2 435 5473 10489 1. 36 5. OE-42 BE217913. 1 EST_HUMAN hv31e11.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3175052 3' 483 5520 8. 24 5.0E-42 5730038 NT Homo sapiens SET domain and mariner transposase fusion gene (SETMAR) mRNA 484 5521 3. 56 5. OE-42 5730038 NT Homo sapiens SET domain and mariner transposase fusion gene (SETMAR) mRNA 744 5767 10792 23. 3 4. OE-42 AF055066. 1 NT Homo sapiens MHC class 1 region 744 5767 10793 23. 3 4.0E-42 AF055066. 1 NT Homo sapiens MHC class 1 region 1049 6058 11087 4. 34 4. OE-42 AF189011. 1 NT Homo sapiens ribonuclease III (RN3) mRNA, complete cds 4071 9065 14055 1. 98 4. OE-42 X59417. 1 NT H. sapiens PROS-27 mRNA 4106 9100 14087 0. 92 4.0E-42 AF246219. 1 NT Homo sapiens SNARE protein kinase SNAK mRNA, complete cds 4127 9122 14107 4. 17 4.0E-42 4506496 NT Homo sapiens regulatoryfactor X, 4 (influences HLA class 11 expression) (RFX4) mRNA 4451 9441 14422 13. 19 4. OE-42 4508008 NT Homo sapiens zinc finger protein 177 (ZNF177) mRNA Page 97 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon top Hit ORF SEQ expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor Valut Value ab14e10.s1 Stratagene lung(#937210) Homo sapiens cDNA clone IMAGE:8409810 3' similar to contains 104 5181 0.9 3.0E-42 AA486105.1 ESF_HUMAN THR.12 THR repelitive element; 1452 6449 115101 2. 61 2. OE-42 BF376834. 1 EST HUMAN RCO-TN0079-110900-024-g07 TN0079 Homo sapiens cDNA 2346 7320 2. 82 2. OE-42 AW898344. 1 EST HUMAN RC3-NN0070-270400-011-h10 NN0070 Homo sapiens cDNA 2358 7332 12448 4. 89 2. OE-42 AW250059. 1 EST HUMAN 2819293. 3prime NIH MGC 7 Homo sapiens cDNA clone IMAGE : 2819293 3' 724 5746 10767 2. 19 1. OE-42 X57147. 1 NT Human endogenous retrovirus pHE. 1 (ERV9) 1025 6035 11067 1. 09 1. OE-42 AW295809. 1 EST_HUMAN UI-H-Bl1-afh-e-04-0-UI.s1 NCI_CGAP_sub3 Homo sapiens cDNA clone IMAGE : 2721871 3' 1084 6091 11120 1. 08 1. OE-42 AJ251818. 1 NT Homo sapiens partial C9 gene for complement component C9, exon1 1084 6091 11121 1. 08 1. OE-42 AJ251818. 1 NT Homo sapiens partial C9 gene for complement component C9, exon 1 Homo sapiens NADH-ubiquinone oxidoreductase AGGG subunit precursor homolog mRNA, nuclear gene 1223 7742 11267 11. 95 1. OE-42 AF067166. 1 NT encoding mitochondrial protein, complete cds Homo sapiens NADH-ubiquinone oxidoreductase AGGG subunit precursor homolog mRNA, nuclear gene 1223 7742 11268 11.95 1.0E-42 AF067166.1 NT encoding mitochondrial proitein, complete cds 1661 6657 11731 1. 13 1. OE-42 11423219 NT Homo sapiens rec (LOC51201), mRNA 2473 7442 12557 1. 26 1. OE-42 5174458 NT Homo sapiens major histocompatibility complex, class II, DM alpha (HLA-DMA) mRNA Homo sapiens origin recognition complex, subunit 5 (yeast homolog)-like (ORC5L) mRNA, and translated 2894 7913 12934 5. 85 1. OE-42 4505524 NT products 3626 8633 13638 2. 28 1. 0E-42 7662027 NT Homo sapiens KIAA0255 gene product (KIaA0255), mRNA# 3705 8709 13712 0. 92 1. OE-42 5031610 NT Homo sapiens Golgi vesicular membrane trafficking protein p18 (BET1) mRNA 3825 8827 13834 1. 08 1. OE-42 AL163267. 2 NT Homo sapiens chromosome 21 segment HS21 C067 4124 9119 13834 1. 89 1. OE-42 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21 C080 4462 9452 14433 0. 75 1. 0E-42 AW813617.1 EST_HUMAN RC3-ST0197-161099-012-a03 ST0197 Homo sapiens cDNA 4602 9590 14577 2. 94 1. OE-42 5803122 NT Homo sapiens proteasome inhibitor (PI310, mRNA 4602 9590 14578 2. 94 1. OE-42 5803122 NT Homo sapiens proteasome inhibitor (Pl31 j, mRNA 4634 9619 14611 5. 64 1. OE-42 4506758 NT Homo sapiens ryanodine receptor 3 (RYR3) mRNA 644 5672 10676 12. 63 8. 0E-43 AV736824. 1 EST HUMAN AV736824 CB Homo sapiens cDNA clone CBLAKH08 5' 644 5672 10677 12. 63 8. OE-43 AV736824. 1 EST HUMAN AV736824 CB Homo sapiens cDNA clone CBLAKH08 5' 691 5715 10729 5. 28 8. 0E-43 8923276 NT Homo sapiens hypothetical protein FLJ20297 (FLJ20297), mRNA 691 5715 10730 5. 28 8. 0E-43 8923276 NT Homo sapiens hypothetical protein FLJ20297 (FLJ20297), mRNA 691 5715 10731 5. 28 8. 0E-43 8923276 NT Homo sapiens hypothetical protein FLJ20297 (FU20297), MRNA 3556 8563 13569 8. 21 7. OE-43 AW246442. 1 EST HUMAN 2822251. 5prime NIH MGC 7 Homo sapiens cDNA clone IMAGE : 2822251 5' ne72dO6. sl NCI-CGAP-Ewl Homo sapiens cDNA clone IMAGE : 909803 similar to gb : L05095 60S 1324 6322 24. 66 6. 0E-43 AA491890. 1 EST HUMAN RIBOSOMAL PROTEIN L30 (HUMAN) ; 2516 7484 2. 96 6. 0E-43 AV708201. 1 EST HUMAN AV708201 ADC Homo sapiens cDNA clone ADCACC10 5' Page 98 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon OrF SEQ Expression (Top) Hit top Hit Acession top Hit SEQ ID ORF SEQ Expression (Top) Hit top run Auession Databace Top Hit Descriptor NO : NO : Signal BLAST E No. source NO: NO: Source Value 142 5208 1.96 5.0E-43 AL163213.2 NT Homo sapiens chromosome 21 segment HS21C013 499 5535 10541 3.04 5.0E-43 AA382780.1 EST_HUMAN EST96033 Testis Homo sapiens cDNA 5'end 27737794 128141. 62 5. OE-43 AV732578. 1 EST HUMAN AV732578 HTF Homo sapiens cDNA crone HTFANC06 5' Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat 958 7696 110071 5. 71 4. OE-43 AF003528. 1 NT regions Homo sapiens calcium channel alpha1 E subunit (CACNA1E) gene, exons 7-49, and partial cds, alternatively 1194 6195 3.19 3.0E-43 AF223391. 19 | spliced 1656 6652 11724 4. 45 3. OE-43 X97869. 1 NT H. sapiens gene encoding La autoantigen AML1-EVI-1=AML1-EVI-1 fusion protein {rearranged translocation} [human, leukemic cell line SKH1, mRNA 3491 8499 13513 1. 29 3. 0E-43 S69002. 1 NT Mutant, 5938 nt] 4167 9162 14148 0. 69 3. 0E-43 AA548154. 1 EST HUMAN nk55dO6. s1 NCI_CGAP_Pr7 Homo sapiens cDNA clone IMAGE : 1017419 qd61c09. x1 SoarestestisNHT Homo sapiens cDNA clone IMAGE : 1733968 3'similar to contains PTR7. t3 183 5246 21. 1 2. 0E-43 AI190764. 1 EST HUMAN PTR7 PTR7 repetitive element ; 1610 6606 11669 2. 07 1. Oye-43 AF154836. 1 NT Homo sapiens Ras-like GTP-binding protein (RAB27A) gene, exons 1b and 2 1610 6606 11670 2. 07 1. 0E-43 AF154836. 1 NT Homo sapiens Ras-like GTP-binding protein (RAB27A) gene, exons 1b and 2 1666 6662 11737 1. 71 1. 0E-43 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21C084$BF348283. 26527612 12722 4. 87 1. 0E-43 NCI 1 EST-HUMAIN 602022313F1 CGAP Brn67 Homo sapiens cDNA clone IMAGE : 4157666 5' 8791 5897 10938 4.62 8.0E-44IAI222985. 1 ESTHUMAN qh23g01. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 1845552 3' 879 5897 10939 4.62 8.0E-44 AI222985. 1 EST HUMAN qh23g01. x1 Soares NFL_T_GBC S1 Homo sapiens cDNA clone IMAGE : 1845552 3' 4721 9706 14692 1. 2 8. OE-44 AW373185. 1 ESTHUMAN RC5-BT0503-081299-011-g12 BT0503 Homo sapiens cDNA 4721 9706 14693 1. 2 8. OE-44 AW373185. 1 ESTHUMAN RC5-BT0503-081299-011-g12 BT0503 Homo sapiens cDNA 651 5679 1. 08 7. 0E-44 R06035. 1 EST HUMAN ye89e1. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 124920 5' 2172 7151 12270 1. 31 7. 0E-44 5031886 NT Homo sapiens LIM domain-containing preferred translocation partner in lipoma (LPP) mRNA 2896 7915 12935 2. 47 7. OE-44 AF048729. 1 NT Homo sapiens minisatellite ms32 repeat region 2896 7915 12936 2. 47 7. 0E-44 AF048729. 1 NT Homo sapiens minisatellite ms32 repeat region 3772 8775 13779 2. 74 7. OE-44 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 41199H3 14098 1. 21 7.0E-44 AF231919.1 NT Homo sapiens chromosome 21 unknown mRNA 4119 9113 14099 1. 21 7. OE-44 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 301 5358 3.07 5.0E-44 AJ289880.1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and LZTFL1 gene 330 5382 1. 86 5. 0E-44 AJ289880. 1 NT Homo sapiens KIAA0851 gene (partial), XT3 gene and LZTFL1 gene 3330 8340 13358 3. 09 4. OE-44 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 4854 9835 1.24 4. OE-44 AI435225. 1 EST HUMAN Iti11d02.x1 NCI_CGAP_Pan1 Homo sapiens cDNA clone IMAGE : 2130147 3' 1748 6742 1. 97 3. OE-44 6912477 NT Homo sapiens karyopherin alpha 6 (importin alpha 7) (KPNA6), mRNA 2460 7429 12546 1. 98 3. 0E-44 BE880626. 1 EST HUMAN 601491529F1 NIH_MGC_69 Homo sapiens cDNA clone IMAGE : 3893839 5' Page 99 of 209 Table 4 Single Exon Probes Ecpressed in HBL100 Cells Probe Exon Most Similar SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession TP Hit ID NO : Signai BLAST E No. Database Top Hit Descriptor NO : NO : Value Source. 3024 8041 13050 6. 08 3. OE-44 AA169851. 1 EST HUMAN zp18b05. r1 Stratagene fetal redna 937202 Homo sapiens cDNA clone IMAGE : 609777 5' 1032 6042 11071 2. 58 2. OE-44 4826685 NT Homo sapiens DEADIH (Asp-Glu-Ala-Asp/His) box polypeptide 1 (DDX1) mRNA 1032 6042 11072 2. 58 2. OE-44 4826685 NT Homo sapiens DEAD/H (Asp-Glu-Ala-Asp/His) box polypep6de 1 (DDX1) mRNA 1188 6189 11226 4. 87 2. OE-44 5803200 NT Homo sapiens transmembrane trafficking protein (TMP21), mRNA 1188 6189 11227 4. 87 2. OE-44 5803200 NT Homo sapiens transmembrane trafficking protein ('fMP21), mRNA 9293 6291 11337 4. 09 2. OE-44 AF133588. 1 NT Homo sapiens RAB36 (RqB36) mRNA, complete cds hw14g06. x1 NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3182938 3'similar to SW : OXYB I-IUMAN 1351 6348 11398 1. 58 2. OE-44 BE465325. 1 EST HUMAN P22059 OXYSTEROL-BINDING PROTEIN. 2091 7072 12186 2. 43 2. OE-44 AF070651. 1 NT Homo sapiens 6ssutype bone marrow zinc finger protein 4 mRNA, complete cds 2536 7501 3. 54 2. OE-44 5901933 NT Homo sapiens adaptor related protein complex 4, sigma 1 subunit (CLAPS4), mRNA 3390 8398 13424 1. 97 2. OE-44 D87675. 1 NT tfomo sapiens DNA for amyloid precursor protein, complete cds 4437 9427 14411 1. 65 2. OE-44 AW864379. 1 EST HUMAN PM4-SN0016-120500-003-a04 SN0016 Homo sapiens cDNA 4644 9629 14623 1. 01 2. OE-44 7706128 NT Homo sapiens oxysterol 7alpha-hydroxylase (CYP39A1), mRNA 4644 9629 14624 1. 01 2. OE-44 7706128 NT Homo sapiens oxysterol 7alpha-hydroxylase (CYP39A1), mRNA 53 5134 10130 8. 52 1. OE-44 7657334 NT Homo sapiens Misshapen/NIK-related kinase (MINK), mRNA 53 5134 10131 8. 52 1. OE-44 7657334 NT Homo sapiens Misshapen/NIK-related kinase (M1NK), mRNA 575 5607 90606 1. 72 1. OE-44 AW853132. 1 EST HUMAN RC1-CT0249-030300-026-h12 CT0249 Homo sapiens cDNA 1178 6180 1. 29 1. OE-44 AW994803. 1 EST HUMAN RC1-BN0039-110300-012-b01 BN0039 Homo sapiens cDNA 1539 6537 3. 96 1. OE-44 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 i zw53d02. r1 Soares total fetus Nb2HF8 9w Homo sapiens cDNA clone IMAGE : 773763 5'similar to 2163 7142 12259 3. 27 1. OE-44 AA434554. 1 EST HUMAN contains THR. t3 THR repetitive element ; zw53d02. r1 Soares total fefus Nb2HF8 9w Homo sapiens cDNA clone IMAGE : 773763 2163 7142 12260 3. 27 1. OE-44 AA434554. 1 EST HUMAN contains THR. i3 THR repetitive element ; Homo sapiens transcription factor IGHM enhancer 3, JM11 protein, JM4 protein, JM5 protein T54 protein, JM10 protein, A4 differentia6on-dependent protein, triple LIM domain protein 6, and synaptophysin genes, 2685 7643 12758 1, 26 1. OE-44 AF196779. 1 NT complete cds ; and L-type calcium channel a> 3642 8648 4. 21 1. OE-44, 4A455869. 1 EST HUMAN aa01c09. s1 Soares NhHMPu S1 Homo sapiens cDNA clone IMAGE : 811984 3' 4945 9922 14900 0. 76 9. OE-44 AJ130755. 9 fVT Homo sapiens alpha satellite DNA, M1 monomer type 4945 9922 14901 0. 76 1. OE-44 AJ130755. 1 NT Homo sapiens alpha satellite DNA, M1 monomer type 4445 9435 14418 1. 61 9. OE-45 8922391 NT Hano sapiens hypothetical protein FLJ10379 (FLJ10379), mRNA 4445 9435 14419 1. 61 9. OE-45 8922391 NT Homo sapiens hypothetica protein FLJ10379 (FLJ10379), mRNA 2453 7423 12538 4. 1 8. OE-45 5174718 NT Homo sapiens TRK-fused gene (NOTE : non-standard symbol and name) (TFG) mRNA--' 4910 9889 14862 8. 47 8. OE-45 5174718 NT Homo sapiens TRK-fused gene (NOTE : non-sfandard symbo ! and name) (TFG) mRNA : s 1. 91 7. OE-45 AL160131. 1 NT Novel human gene mapping to Page 100 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ @ID SEQ ID ID NO: Signal BLASTE No. Source Top Hit Descriptor Value wb99c06.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE : 2313802 3'similar to contains L1. t1 L1 1521 6518 1. 01 6. 0E-45 AI675425.1 EST_HUMAN repetitive element ; au83h07.x1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE:2782909 3' simllar to 3871 8872 9. 19 6. 0E-45 AW 157570. 1 EST HUMAIN SW : R13AHUMAN P40429 60S RIBOSOMAL PROTEIN L13A ; 881 5899 1. 17 5. OE-45 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21C003 1956 6942 12044 2. 41 5. OE-45 BF333627. 1 EST HUMAN CM4-CN0044-180200-515-f01 CN0044 Homo sapiens cDNA tg94f07.x1 NCI_CGAP_CLL1 Homo sapiens cDNA clone IMAGE : 2116453 3'similar to SW : PAX1 MOUSE 3137 8153 13176 2. 09 5. 0E-45 AI523766. 1 EST HUMAN P09084 PAIRED BOX PROTEIN PAX-1.; 1126 6131 11161 13. 11 4. 0E-45 X95826.1 NT H. sapiens ART4 gene 2229 7206 12321 3. 69 4. OE-45 BE265622. 1 EST_HUMAN 601194440F1 NIH_MGC_7 Homo sapiens cDNA clone IMAGE : 3538425 5' 3946 8944 13934 1. 07 4. 0E-45 4503422 NT Homo sapiens dUTP pyrophosphatase (DUT) mRNA 3256 8260 1.58 3.0E-45 T71480.1 EST_HUMAN yd35f07.r1 Soares fetal liver epleen 1NFLS Homo sepiens cDNA clone IMAGE:110245 5' 3971 8269 1.768 3.0E-45 T71480.1 EST_HUMAN yd35f07.r1 Soares fetal liver spieen 1NFLS Homo sapiens cDNA clone IMAGE:110245 5' 2429 7400 1.54 2. 0E-45 AL163218. 2 NT Homo sapiens chromosome 21 segment HS21C018 2962 7980 12994 1. 28 2. 0E-45 AJ243213. 1 NT Homo sapiens partial 5-HT4 receptor gene, exons 2 to 5 124 5443 3. 37 1. 0E-45 BE389855.1 EST_HUMAN 601284360F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3606183 5' 406 5443 3. 74 1.0E-45 BE389855. 1 EST HUMAN 601284360F1 NIH MGC 44 Homo sapiens cDNA clone IMAGE : 3606183 5' 469 5505 10519 1. 3 1. OE-45 4506412 NT Homo sapiens RAP1A, member of RAS oncogene family (RAP1A), mRNA 1155 6159 11193 1. 76 1. OE-45 7657290 NT Homo sapiens Langerhans cell specific c-type lectin (LANGERIN), mRNA 3030 8047 13057 7. 83 1. 0E-45 U3216S. 1 NT Human pro-a2 chain of collagen type XI (COL11A20 gene, co9mplete cds 3412 8421 13450 0. 79 1. OE-45 8659558 NT Homo sapiens chromosome 21 open reading frame 1 (C21orf4), mRNA 4350 9341 14322 4. 49 1. OE-45 BE396633. 1 EST HUMAN 601289116F1 NIH MGC 8 Homo sapiens cDNA clone IMAGE : 3619803 5' 632f08. x1 NCI CGAP_Gas4 Homo sapiens cDNA clone IMAGE : 2132199 3'similar to gb : J00314 ma2 2374 7346 12466 26. 07 8. 0E-46 Al433261. 1 EST HUMAN TUBULIN BETA-1 CHAIN (HUMAN) ; ti32fO8. x1 NCI_CGAP_Gas4 Homo sapiens cDNA clone IMAGE : 2132199 3'similar to gb : J00314_rna2 2374 7346 12467 26. 07 8. 0E-46 AI433261. 1 EST HUMAN TUBULIN BETA-1 CHAIN (HUMAN) ; 2177 7156 12276 1. 08 7. OE-46 U46007. 1 NT Rattus norvegicus espin mRNA, complete cds 4447 9437 9. 38 7. OE-46 BE386165. 1 EST HUMAN 601277292F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3618119 5' 4662 9647 1. 73 7. 0E-46 BE064386. 1 ESTHUMAN RC4-BT0310-110300-015-f10BT0310 Homo sapiens cDNA wm31f08.x1 NCI_CGAP_Ut4 Homo sapiens cDNA clone IMAGE : 2437575 3'similar to contains MER19. t2 2681 7639 12754 3. 59 6. OE-46 AI884381. 1 EST HUMAN MER19 repetitive element ; wm31f08. x1 NCiCGAPUt4 Homo sapiens cDNA clone IMAGE : 2437575 3'similar to contains MER19. t2 2681 7639 12755 3.59 6. 6. CE-46 A1884381. 1 EST HUMAN MER19 repetitive element ; 204 5268 4. 44 5. OE-46 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21 C010 Page 101 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hif SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor Value 3450 8458 13484 1. 07 5. 0E-46 BE677194. 1 EST_HUMAN 7d81g01. x1 Lupski_dorsal_root_ganglion Homo sapiens cDNA clone IMAGE : 3279408 3' 3450 8458 13485 1. 07 5. OE-46 BE677194. 1 EST_HUMAN 7d81g01.x1 Lupski_dorsal_root_ganglion Homo sapiens cDNA clone IMAGE : 3279408 3' no54e09. s1 NCtCGAPSS1 Homo sapiens cDNA clone IMAGE : 1104520 3' similar to gb : X53741_rna1 635 5663 2. 5 4. 0E-46 AA601143. 1 EST HUMAN FIBULIN-1, ISOFORM A PRECURSOR (HUMAN) ; hl86c03.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3008836 3'similar to gb : X14008_rna 1665 6661 11735 8. 01 4. 0E-46 AW770544. 1 EST HUMAN LYSOZYME C PRECURSOR (HUMAN) ; contains element MER37 repetitive element ; hl86c03.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3008836 3'similar to gb : X14008 ma1 1665 6661 11736 8. 01 4. 0E-46 AW770544. 1 EST HUMAN LYSOZYME C PRECURSOR (HUMAN) ; contains element MER37 repetitive element ; 2666 7625 12737 2. 58 4. OE-46 M18048. 1 NT Human endogenous retrovirus RTVL-H2 4293 9285 14272 0. 97 4. OE-46 AB014522. 1 NT Homo sapiens mRNA for KIAA0622 protein, partial cds 4293 9285 14273 0. 97 4. OE-46 AB014522. 1 NT Homo sapiens mRNA for KIAA0622 protein, partial cds 2222 7199 12319 1. 23 3. OE-46 7657203 NT Homo sapiens acidic 82 kDa protein mRNA (HSU15552), mRNA 2361 7335 12452 2. 18 3. 0E-46 AF160212. 1 NT Homo sapiens VAMP-associated 33 kDa protein mRNA, complete cds 4269 9262 14252 0. 72 3. 0E-46 4506376 NT Homo sapiens mitogen-acvated protein kinase kinase kinase kinase 3 (MAP4K3), mRNA 4630 9615 14605 1. 22 3. OE-46 Z73660. 1 NT H. sapiens 19 lambda light chain variable region gene (7c. 11. 2) germline ; Ig-Light-Lambda ; VLambda 4630 9615 14606 1. 22 3. OE-46 Z73660. 1 NT H. sapiens 19 lambda light chain variable region gene (7c. 11. 2) germline ; Ig-Light-Lambda ; Lambda ne06a09. s1 NCI_CGAP_Co3 Homo sapiens cDNA clone IMAGE : 880408 3'similar to contains THR. b2 THR 827 5847 10884 8. 39 2. 0E-46 AA468646.1 EST_HUMAN repetitive element ; Homo sapiens Bruton's tyrosine kinase ( 1601 6597 11658 2. 53 2. OE-46 U78027. 1 NT (L44L) and FTP3 (FTP3) genes, complete cds zt59e02. r1 Soares_testis_NHt Homo sapiens cDNA clone IMAGE : 726650 5'similar to SW : RSP1MOUSE 4815 9799 147801. 31 2. OE-46 AA399286. 1 EST_HUMAN Q01730. RSP-1 PROtEIN. ; 1213 6212 11252 5. 7 1. OE-46 4502694 NT Homo sapiens cell division cycle 10 (homologous to CDC10 of S. cerevisiae) (CDC10) mRNA 1538 6536 11594 1. 27 1. OE-46 7662177 NT Homo sapiens KIAA0555 gene product (KIAA0555), mRNA 1538 6536 11595 1. 27 1. OE-46 7662177 NT Homo sapiens KIAA0555 gene product (K (AA0555), mRNA 2218 7195 12317 3. 91 1. OE-46 AW978516 1 EST HUMAN EST390625 MAGE resequences, MAGP Homo sapiens cDNA 2336 7310 12431 2. 62 1. OE-46 H97330. 1 ESTHUMAN EST48b095 WATM1 Homo sapiens cDNA clone 48bO95 np78b02. s1 NCLCGAPPr2 Homo sapiens cDNA clone IMAGE : 1132395 similar to gb : X76717 H. sapiens 3176 8192 13213 8. 47 1. OE-46 AA631912. 1 EST_HUMAN MT-1 mRNA. (HUMAN) ; 4723 9708 3 1. OE-46 AB023197. 1 NT Homo sapiens mRNA for KIAA0980 protein, partial cds Page 102 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon OrF SEQ Expression (Top) Hit Top Hit Acession Top Jit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Value 757 5778 3. 81 9. OE-47 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1/2 hl93e04. x1 NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3009534 3'similar to TR : 075703 075703 4776 9760 14747 2. 41 9.0E-47 AW770928.1 EST_HUMAN HYPOTHETICAL 12.4 KD PROtEIN.: 1772 6764 11852 90. 6 8.0E-47 Y18536. 1 NT Homo sapiens HLA-C gene, exon 5, individual 19323 1772 6764 11853 90. 6 8. 0E-47 Y18536. 1 NT Homo sapiens HLA-C gene, exon 5. individual 19323 2644 7604 12717 1.38 8.0E-47 5453955 NT Homo saplens prolein phosphalase 2, regulatory subunit B (B56), epsilon isoform (PPP2R5e) mRNA 2957 7976 12991 1.73 8.0E-47 AJ229043.1 NT Homo sapiens 959 kb contig between AML1 and cBR1 on chromosome 21q22, segment 3/3 2477 7466 12559 1. 23 6. OE-47 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 1376 6373 11423 5. 93 4. OE-47 4557556 NT Homo sapiens E1A binding protein p300 (EP300) mRNA 540 5575 10580 3. 84 3. OE-47 BE907634. 1 EST_HUMAN 601497639F1 NH_MGC_70 Homo sapiens cDNA clone IMAGE : 3899721 5' 540 5575 10581 3. 84 3, OE-47 BE907634. 1 EST_HUMAN 601497639F1 NIH_MGC_70 Homo sapiens cDNA clone MAGE : 3899721 5' 808 5829 10860 4. 98 3. OE-47 N57483. 1 EST_HUMAN yy54b04.s1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE : 277327 3' 931 5948 10982 7. 81 3. OE-47 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21C084 3230 8245 13267 0. 94 3. OE-47 4504116 NT Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1) mRNA 3859 8861 6.7 3.0E-47 U93181. 1 NT Homo sapiens 1 phosphatase Homo sapiens 4237 9231 14214 1. 253. 0E-47 M12959. 1 NT Human T-cell receptor active alpha-chain mRNA from JM cell line, complete cds 147 5213 10227 1. 29 2.0E-47 4505318 NT Homo sapiens myosin phosphatase, target subunit 2 (MYPT2), mRNA 953 5969 11001 2. 67 2.0E-47 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21 C009 953 5969 11002 2. 67 2.0E-47 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21 C009 1558 6555 11616 2 2. OE-47 7662109 NT Homo sapiens KIAA0426 gene product (KIAA0426), mRNA 1638 6635 11705 3. 76 2.0E-47 AA524514. 1 EST HUMAN ng43h12. s1 NCI CGAP_Co3 Homo sapienscDNA clone IMAGE : 937607 3' 4221 9215 14194 1.6 2.0E-47 4504866 NT Homo sapiens ring finger protein (C3HC4 type) 8 (RNF8), mRNA 4259 9253 14240 1. 822. 0E-47 AA569592. 1 EST_HUMAN nf23g07.s1 NCI_CGAP_Pr1 Homo sapiens cDNA clone IMAGE : 914652 4259 9253 14241 1. 82 2.0E-47 AA569592. 1 EST HUMAN nf23g07. s1 NCI_cGAP_Pr1 Homo sapiens cDNA done) IMAGE : 914652 4376 9367 14347 2. 23 2.0E-47 5174648 NT Homo saplens Rev/Rex activation domain binding protein-related (RAB-R) mRNA 4667 9652 14640 1.32 2.0E-47 AW965166. 1 EST_HUMAN EST377239 MAGE resequences, MAGI Homo sapiens cDNA 1381 6378 11426 4.13 1.0E-47 AI333429.1 EST_HUMAN qp99h03.x1 Soares_fetal_lung_NbHL 19W Homo saples cDNA clone IMAGE:1931189 3' 3731 8735 13732 2.17 1.0E-47 BE280477. 1 EST HUMAN 601155321 F1 NIH_MGC 21 Homo sapiens cDNA clone IMAGE : 3138893 5' 3731 8735 13733 2.17 1.0E-47 BE28047. 1 ESt_HUMAN 601155321F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3138893 5' 4904 9883 14854 3.23 1.0E-47 AW813906.1 EST_HUMAN RC3-sT0197-130400-017-h02 ST0197 Homo sapiens cDNA Homo sapiens calcium channel alpha1E subunit (CACNA1 E) gene, exons 7-49, and partial cds, alternatively 1575 6572 11633 3. 85 9 OE48 AF223391. 1 NT spliced 1231 6230 1. 49 8.0E-48 4501900 NT Homo sapiens aminoacyiase 1 (ACY1), mRNA Page 103 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit Probe Exon ar r-/-r \..--r L. A'TopH ! t SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Database Top Hit Descriptor Source, NO : NO : Value 1232 6230 1. 54 8. OE-48 4501900 NT Homo sapiens aminoacylase 1 (ACY1), mRNA hk61b03. x1 NCI CGAP Lym12 Homo sapiens cDNA clone IMAGE : 3001133 3'similar to gb : X64707 3062 8079 13092 3. 91 8. OE-48 AW 768477. 1 EST HUMAN BREAST BASIC CONSERVED PROTEIN 1 (HUMAN) ; hk61b03. x1 NCI CGAP Lym12 Homo sapiens cDNA clone IMAGE : 3001133 3'similar to gb : X64707 3062 8079 13093 3. 91 8. OE-48 AW768477. 1 EST HUMAN BREAST BASIC CONSERVED PROTEIN 1 (HUMAN) ; 487 5524 1. 31 7. OE-48 AB033035. 1 NT Homo sapiens mRNA for KIAA1209 protein, partial cds 488 5524 19. 03 7. OE-48 A8033035. 1 NT Homo sapiens mRNA for KIAA1209 protein, partial cds 1467 6464 11523 1. 05 7. OE-48 6912719 NT Homo sapiens tousled-like kinase 1 (TLK1), mRNA 1597 6593 11654 5. 73 7. OE-48 5730038 NT Homo sapiens SET domain and mariner transposase fusion gene (SETMAR) mRNA 3518 8526 13537 7. 99 6. OE-48 AI761111. 1 EST HUMAN wi69h03. c'1 NCI_CGAP Kid12 Homo sapiens cDNA clone IMAGE : 2398613 3' 3237 10046 13272 1. 51 S. OE-48 4826891 NT Homo sapiens phosphodiesterase 1A, calmodulin-dependent (PDE1A) mRNA 1931 6917 12015 28. 02 3. OE-48 4885170 NT Homo sapiens chromosome X open reading frame 6 (CXORF6) mRNA 1931 6917 12016 28. 02 3. OE-48 4885170 NT Homo sapiens chromosome X open reading frame 6 (CXORF6) mRNA 4125 9120 0. 99 3. OE-48 AA009541. 1 EST HUMAN zi04g03. r1 Soares fetal liver spleen 1NFLS S1 Homo sapiens cDNA clone IMAGE : 429844 5' 5 5086 10071 1. 14 2. OE-48 AA465007. 1 EST HUMAN ac8c03. r1 Soares ovary tumor NbHOT Homo sapiens cDNA clone IMAGE : 810052 5' 46 5127 1011 S 2. 18 2. OE-48 AA631940. 1 EST HUMAN fmfc7 Regional genomic DNA specc cDNA library Homo sapiens cDNA clone CR17-26 TCBAP1D3842 Pediatric pre-B cell acute lymphoblastic leukemia Baylor-HGSC project=TCBA Homo 4401 9392 14376 0. 95 2. OE-48 BE246065. 1 EST_HUMAN sapiens cDNA clone TCBAP3842 4803 9787 14769 1. 1 2. OE-48 T03176. 1 EST HUMAN F82E2 Fetal brain, Stratagene Homo sapiens cDNA clone FB2E2 3'end 4803 9787 14770 1. 1 2. OE-48 T03176. 1 EST HUMAN FB2E2 Fetal brain, Stratagene Homo sapiens cDNA cione FB2E2 3'end, 5064 10033 15000 1. 11 2. OE-48 AW 470877. 1 EST HUMAN m67a10. x1 NCI CGAP GC6 Homo sapiens cDNA clone IMAGE : 2689242 3' 57 5137 10135 8. 01 1. OE-48 7706534 NT Homo sapiens cispla6n resistance-associated overexpressed protein (LOC51747), mRNA 862 5881 10922 4. 37 1. OE-48 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nepn-II, Alzheimer disease) (APP), mRNA 1059 6068 11098 3. 2 1. OE-48 7657430 NT Homo sapiens EBNA-2 co-activator (100kD) (p100), mRNA 1059 6068 11099 3. 2 1. OE-48 7657430 NT Homo sapiens EBNA-2 co-activator (100kD) (p100), mRNA 6276 11317 4. 07 1. OE-48 5032032 NT Homo sapiens RNA binding moFrf protein 6 (RBM6) mRNA 1874 6863 11952 15. 16 1. OE-48 AL163302. 2 NT Homo sapiens chromosome 21 segment HS21 C102 3408 8417 13445 1. 25 1. OE-48 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 4959 9936 14914 0. 91 1. OE-48 M10976. 1 NT Human endogenous retroviral DNA (4-1), complete retrovial segment 1959 6945 12047 0. 92 8. OE-49 A8026497. 1 NT Mus musculus MysPDZ mRNA for myosin containing PDZ domain, complete cds 139 5430 10445 4. 24 7. OE-49 5729990 NT Horno sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4) mRNA 139 5430 10446 4. 24 7. OE-49 5729990 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4) mRNA 3g2 5430 10445 3. 24 7. OE-49 5729990 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4) mRNA Page 104 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit Probe Exon ORF SEQ Expression SEQ ID SEQ ID ORF SEQ Expression (Top) Hit top Hit acession Database Top Hit Describptor NO : NO : ID NO: Signal BLASTE No. Source Value 392 5430 10446 3. 24 7. 0E-49 5729990 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4) mRNA 393 5430 10445 2. 99 7. 0E-49 5729990 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4) mRNA 393 5430 10446 2. 99 7. 0E-49 5729990 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4) mRNA 1201 6202 11239 3. 59 7. 0E-49 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 ba55g05. x1 NIH_MGC_10 Homo sapiens cDNA clone IMAGE : 2900504 3'similar to gb : X17206 40S RIBOSOMAL PROTEIN S4 (HUMAN) ; gb : M20632 Mouse LLRep3 protein mRNA from a repetitive element, 197 5261 10274 180. 31 6. OE-49 AW731740. 1 EST HUMAN complete (MOUSE) ; 1340 6338 11387 0. 99 6. OE-49 BF038269. 1 EST_HUMAN 601457738F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3861272 5' 1340 6338 11388 0. 99 6. OE-49 BF038269. 1 EST_HUMAN 601457738F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3861272 5' 3999 8995 13983 0. 98 6. OE-49 AL162091. 1 EST_HUMAN DKFZp761A138_s1 761 (synonym : hamy2) Homo sapiens cDNA clone DKFZp761A138 3' 702 5726 10741 7. 25 5. OE-49 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 702 5726 10742 7. 25 5. 0E-49 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 zp29c07. r1 Stratagene neuroepithelium (#937231) Homo sapiens cDNA clone IMAGE : 610860 5'similar to 1758 6751 11835 3. 85 5. 0E-49 AA172121. 1 EST HUMAN TR : G233226 G233226 RTVL-H PROTEIN. ; contains LTR7. t3 LTR7 LTR7 repetitive element ; 2677 7635 12750 7. 57 5. 0E-49 U17714.1 NT Homo sapiens putative tumor suppressor ST13 (sT13) mRNA, complete cds 3199 8215 13236 9.74 5.0E-49 11436355 NT Homo saplens slmllar to ribosomal protein S27 (metallopanstimulln 1) (H. saplens) (LOC63362), mRNA xI06b01.x1 NCI_CGAP_Ut4 Homo saplens cDNA clone IMAGE:2675593 3' similer to WP:B0350.2B 521 5556 10558 22. 86 4. 0E-49 AW189533. 1 EST_HUMAN CE06703 ; 556 5590 10591 0. 9 3. OE-49 X68968. 1 NT H. sapiens mRNA for acetyl-CoA carboxylase ze31c05. r1 Scares retina N2b4HR Homo sapiens cDNA clone IMAGE:360584 5' similar to contains L1.t3 L1 2575 7538 1. 21 3. 0E-49 AA016131. 1 EST HUMAN repetitive element ; 4824 9808 14790 2. 5 3. OE-49 U46999. 1 NT Human type IV collagen (COL4A6) gene, exon 40 4999 9970 0. 94 3. OE-49 L78810. 1 NT Homo sapiens ADP/ATP carrier protein (ANT-2) gene, complete cds 653 5681 3. 06 2. OE-49 BE165980. 1 EST HUMAN MR3-HT0487-150200-113-g01 HT0487 Homo sapiens cDNA 3151 8167 13187 1. 44 2. 0E-49 N26446. 1 EST HUMAN yx23d06. r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 262571 5 888 5906 5. 66 1. 0E-49 BF035327.1 EST_HUMAN 601458531F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3862086 5' 1522 6519 11575 27. 09 1. 0E-49 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA 1765 6757 11843 5. 52 1. OE-49 BE255216. 1 EST HUMAN 601115769F1 NIH MGC 16 Homo sapiens cDNA clone IMAGE : 3356273 5' 169 5234 10244 2. 76 8. 0E-50 AL163202. 2 NT Homo sapiens chromosome 21 segment HS21 C002 709 5733 10749 1. 82 8. 0E-590 X95097. 2 NT Homo sapiens mRNA for VIP receptor 2 709 5733 10750 1. 82 8. 0E-50 X95097. 2NTHomo sapiens mRNA for VIP receptor 2 1726 6721 11799 13. 5 8. 0E-50 4501890 NT Homo sapiens actinin, alpha 1 (ACTN1) mRNA Page 105 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit ORF SEQ Expresslon (Top) Hit top Hit Acesslon SEQ ID SEQ ID Database top Hit Descriptor ID NO: signal BLASTE No. Value 2411 7382 12500 1. 29 8. OE-50 7706394 NT Homo sapiens p47 (LOC51674), mRNA 2411 7382 12501 1. 29 8. 0E-50 7706394 NT Homo saplens p47 (LOC51674), mRNA 2627 7587 12699 3. 51 8. OE-50 4826658 NT Homo sapiens capping protein (actin filament) muscle Z-line, beta (CAPZB), mRNA 4217 9210 0. 88 6. 0E-50 BE794381. 1 EST_HUMAN 601589565F1 NIH_MGC_7 - Homo sapiens cDNA clone IMAGE : 3943577 5' 1757 6750 11833 1.19 5.0E-50 BF332938. 1 EST HUMAN CMO-BT0792-300500-398-b05 BT0792 Homo sapiens cDNA 1757 6750 11834 1. 19 5. 0E-50 BF332938. 1 EST HUMAN CMO-BT0792-300500-398-b05 BT0792 Homo sapiens cDNA no54e09. s1 NCI_CGAP_SS1 Homo sapiens cDNA clone IMAGE : 1104520 3'similar to gb : X53741_rna1 906 5923 1.83 4.0E-50 AA601143. 1 EST HUMAN FIBULIN-1, ISOFORM A PRECURSOR (HUMAN) ; 3365 8373 13393 0.98 4. 0E-50 AL163248. 2 NT Homo sapiens chromosome 21 segment HS21 C048 1898 6886 2. 31 3. 0E-50 M18048. 1 NT Human endogenous retrovirus RTVL-H2 2447 7417 12532 1.05 3.0E-50 BE259196. 1 EST_HUMAN 601109717F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE : 3350309 5' 3225 8240 13262 0.89 3.0E-50 AA746142.1 EST_HUMAN ob03f06.s1 NCI_CGAP_Kid3 Homo sapiens cDNA clone IMAGE : 1322627 3' hg26eO1. x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2946744 3'similar to SW : C1TCHUMAN 5032 10003 14974 1. 04 3. 0E-50 AW593866. 1 EST_HUMAN P11586 C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC ;conteins Alu repetitive element ; 769 5790 14.02 2. 0E-50 AF055066. 1 NT Homo sapiens MHC class 1 region 1063 6071 11103 5. 6 2. OE-50 4557752 NT Homo sapiens midline 1 (Opitz/BBB syndrome) (MID1) mRNA 1417 6414 11475 1.29 2.0E-50 AF138303. 1 NT Homo sapiens decorin D mRNA, complete cds, alternatively spliced 3214 8229 13250 0. 78 2. 0E-50 AF111168. 2 NT Homo sapiens serine palmitoyi transferase, subunit 11 gene, complete cds ; and unknown genes 4138 9133 14116 1.11 2.0E-50 D86424. 1 NT Mus musculus mRNA for high-sulfur keratin protein, partial cds 459 5496 10507 1. 74 1. OE-50 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21 C009 2304 7279 7. 62 1. OE-50 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1/2 np98e09. s1 NCI CGAP Lu1 Homo sapiens cDNA clone IMAGE : 1142440 3'similar to gb : X12671 ma1 4435 9425 14410 12. 15 8. 0E-51 AA610842. 1 EST HUMAN HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN A1 (HUMAN) ; xn34a03. x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2695564 3'similar to TR : Q9Z340 2950 7969 12986 1. 33 7. 0E-51 AW274720. 1 EST HUMAN Q9Z340 ATYPICAL PKC SPECIFIC BINDING PROTEIN. ; 3209 8224 13246 1. 63 7. 0E-51 AW889219. 1 ESTHUMAN QV4-NT0028-200400-180-d05 NT0028 Homo sapiens cDNA xn34a03.x1 NCI CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2695564 3'similar to TR : Q9Z340 3282 8294 13319 0.82 7. 0E-51 AW274720. 1 EST HUMAN Q9Z340 ATYPICAL PKC SPECIFIC BINDING PROTEIN. ; 4043 9039 14029 1. 25 7. 0E-51 AL079628. 1 EST HUMAN DKFZp434B2229 r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434B2229 5' 4043 9039 14030 1. 25 7. 0E-51 AL079628. 1 EST HUMAN DKFZp434B2229 r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434B2229 5' 4225 9219 14198 2. 54 7. 0E-51 AW295603. 1 EST HUMAN UI-H-BWO-aip-b-05-0-Ul. s1 NCI CGAP Sub6 Homo sapiens cDNA clone IMAGE : 2729817 3' 1496 6494 11549 1.16 6.0E-51 6678763 NT Homo sapiens putative DNA binding protein (M96), mRNA Page 106 of 209 Table 4 Single Exon Probes Expressed in HBL.100 Cells Most Similar Probe exon top Hit ORF SEQ Expresslon (Top) Hit top Hit Acession SEQ ID SEQ ID ID NO: Sianal BI ASTE NE Database Top Hit Desoriptor Valut Value 1934 6920 12019 3.34 6.0E-51 7657266 NT Homo sapiens KIAA0929 protein Mx2 interacting nuclear tyarget (MINT) homolog (KIAA0929), mRNA 3393 8401 13427 15. 79 6. OE-51 7657266 NT Homo sapiens kIAA0929 protein Msx2 interacting nuclear target (MINT) homolog (KIAA0929), mRNA 783 5804 10834 5. 86 5. 0E-51 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 794 5815 10846 1. 74 5. 0E-51 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 976 7736 11024 1. 07 5. 0E-51 AL133204. 1 NT Novel human gene mapping to chomosome X 1571 6568 11630 1. 42 5. OE-51 5031980 NT Homo sapiens 26S proteasome-associated pad1 homolog (POH1) mRNA 2517 7485 12601 6.01 5.0E-51 AJ007558.1 NT Homo sapiens mRNA for nucleoporin 155 3841 8843 13850 3. 67 5. 0E-51 M30938. 1 NT Human Ku (p70/p80) subunit mRNA, complete cds 3841 8843 13851 3. 67 5. 0E-51 M30938. 1 NT Human Ku (p70/p80) subunit mRNA, complete cds 4900 9879 14848 1. 54 5. 0E-51 AB037832. 1 NT Homo sapiens mRNA for KIAA1411 protein, partial cds tr81c09.x1 NCI_CGAP_Pan1 Homo sapiens cDNA clone IMAGE : 2224720 3'similar to gb : M26326 135 5201 10217 20.61 3.0E-51 AI587348.1 EST_HUMAN KERATIN, TYPEI CYTOSKELETAL 18 (HUMAN); tr81c09.x1 NCI_CGAP_Pan1 Homa sapiens cDNA clons IMAGE:2224720 3' simller to gb:M26326 1157 6161 11195 35. 84 3. OE-51 AI587348. 1 EST HUMAN KERATIN, TYPE I CYTOSKELETAL 18 (HUMAN) ; I 1882 6871 11960 1. 08 3. 0E-51 AA211296. 1 ESTHUMAN zq87g01. s1 Stratagene hNT neuron (#937233) Homo sapiens cDNA clone IMAGE : 649008 3' 4200 9193 14175 2. 01 3. 0E-51 AL159142. 1 NT Nove human gene mapping to chomosome 22 Homo sapiens ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman. 365 5414 10427 2. 45 2. 0E-51 4507798 NT syndrome0 (UBE3A) mRNA 679 5704 10712 1. 65 2. 0E-51 BE391063. 1 EST_HUMaN 601285694F1 NIH_MGC_44 Homo saplens cDNA clone IMAGE:3607463 5' 679 5704 10713 1. 65 2. 0E-51 BE391063. 1 EST_HUMAN 601285694F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3607463 5' zr30a05. r1 Stratagene NT2 neuronal precursor 937230 Homo sapiens cDNA clone IMAGE : 664880 5'similar 1649 6645 11718 6. 36 2. 0E-51 AA233352. 1 EST HUMAN to TR : G233226 G233226 RTVL-H PROTEIN. contains LTR7. t3 LTR7 repetitive element ; 3648 8654 13660 2. 94 2. 0E-51 AI492415. 1 EST_HUMAN ti27g03.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2131732 3' 114 518e 10195 45. 03 1. OE-51 4503528 NT Homo sapiens eukaryofic translation initiation factor 4A, isoform 1 (EIF4A1) mRNA 1462 6459 51. 24 1. OE-51 AV742248. 1 EST_HUMAN AV742248 CB Homo sapiens cDNA clone CBFBCC12 5' nw21g02.s1 NCI_CGAP_GCB0 Homo sapiens cDNA clone IMAGE : 1241138 3'similar to contains THR. t3 151 5217 10230 7. 75 8. 0E-52 AA720574. 1 EST HUMAN THR repetitive element ; 1466 6463 11522 1. 35 8. 0E-52 X84900.1 NT H. sapiens mRNA for laminin-5, alpha3b chain 1614 6610 11674 2. 31 8. 0E-52 11968028 NT Homo sapiens hypothetical protein FLJ13556 similar to N-myc downstream regulated 3 (FLJ13556), mRNA 1614 6610 11675 2. 31 8. 0E-52 11968028 NT Homo sapiens hypothetical protein FLJ13556 similar to N-myc downstream regulated 3 (FLJ13556), mRNA Page 107 of 209 Table 4 Sinnlra Fxnn Prnhac Fvnrceecrl in 1-IRI'll1 (1 f'ellc Most Slmilar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO : Signal BLAST E No. Source Top Hit Desoriptor NO: NO: Source Value 3888 6610 11674 6.2 8.0E-52 11968028 NT Homo sapiens hypolhetical protein FLJ 13556 slmller to N-mye dowmstream regulated 3 (fLJ13556), mRNA 3888 6610 11675 6.2 8.0E-52 11968028 NT Homo sapiens hypothetical protein FLJ13556 similar to N-myc downstream regulated 3 (FLJ13556), mRNA Homo sapiens S164 gene, partial cds ; PS1 and hypothetical protein genes, complete cds ; and S171 gene, 1655 6651 11723 3. 39 6. OE-52 AF109907. 1 NT partial cds 4318 9310 14296 2.8 5.0E-52 Z78898. 1 NT H. sapiens flow-sorted chromosome 6 Hindlil fragment, SC6pA18H7 1622 6619 11685 1. 32 4. OE-52 AF257318. 1 NT Homo sapiens SH3-containing protein SH3GLB1 mRNA, complete cds 1750 6744 11823 2.08 4.0E-52 4758843 NT Homo sapiens nucleoporin 155kD (NUP155) mRNA 3826 8828 13835 0. 81 4.0E-52 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA Homo sapiens 5, 10-methylenetetrahydrofolate dehydrogenase, 5, 10-methylenetetrahydrofolate 4514 9504 14484 1. 26 4.0E-52 5174590 NT cyclohydrolase, 10-formyltertrahydrofolate synthetase (MTHFD) mRNA 3975 8973 10.25 3.0E-52 11437042 NT Homo sapiens hypothetical protein FLJ10675 (FLJ10675), MRNA 558 5592 10592 1. 85 2. OE-52 M10976. 1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 558 5592 10593 1. 85 2. OE-52 M10976. 1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 1718 6713 11790 1. 15 2. OE-52 AB007899. 1 NT Homo sapiens KIAA0439 mRNA, partial cds 1964 6949 12052 1 2. OE-52 AB033075. 1 NT Homo sapiens mRNA for Kit1249 protein, partial cds bb66b07.y1 NIH_MGC_9 Homo sapiens cDNA clone IMAGE : 3030421 5'similar to gb : X16493 M. musculus 2426 7397 12518 3.12 2.0E-52 BE207575. 1 EST HUMAN mRNA for Zpf-1 zinc finger protein (MOUSE) ; 2662 7621 19. 48 2. OE-52 BF677892. 1 EST_HUMAN 602084710F1 NIH_MGC_83 Homo sapiens cDNA clone IMAGE : 4248891 5' 4818 9802 14782 3. 17 2. OE-52 AL137188. 3 NT Novel human gene mapping to chromosome 20, similar to membrane transporters 4853 9834 14808 1.1 2.0E-52 Al141802 1 ESTHUMANqa56e05. s1 SoaresNhHMPuS1 Homo sapiens cDNA clone IMAGE : 1690784 3' 4853 9834 14809 1.1 2.0E-52 AI141802. 1 EST_HUMAN qa56e05.s1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE : 1690784 3' 529 5564 10568 1. 37 1. OE-52 AA634445. 1 EST_HUMAn zu75h12.s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE : 743879 3' 1354 6351 11401 9. 59 1. OE-52 4504026 NT Homo sapiens glutamate-ammonia ligase (glutamin synthase) (GLUL) mRNA 2465 7434 1. 67 1. OE-52 4502238 NT Homo sapiens arytsuifatase D (ARSD), transcript variant 1, mRNA pol=reverse transcriptase homolog {retroviral element} [human, endogenous retroviral element RTVL-Hp1, 2985 8003 13015 1. 99 1. OE-52 S61070. 1 NT Genomic, 660 nt] 3702 8706 13708 1. 3 9.0E-53 4506064 NT Homo sapiens protein kinase, cAMP-dependent, regulatory, type il, beta (PRKAR2B) mRNA 4267 9260 14250 1. 22 9.0E-53 AF001446. 1 NT Homo sapiens core binding factor alpha1 subunit (CBFA1) gene, exon 3 5033 10004 14975 1. 19 9. OE-53 AB040937. 1 NT Homo sapiens mRNA for KIAA1504 protein, partial cds 3982 8980 13965 15.99 5.0E-53 4758543 NT Homo sapiens heterogeneous nuclear ribonucleoprotein C (C1/C2) (HNRPC) mRNA 50 5131 10125 1. 63 4.0E-63 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21C085 5C 5131 10126 1. 53 4.0E-53 AL163265. 2 NT Homo sapiens chromosome 21 segment HS21C085 Page 108 of 209 i'abte 4 Single Exon Probes Expressed in HBL100 Celis Most Similar Probe Exon Top HIt ORF SEQ Expression (Top) Hit top Hit Acession SEQ ID SEQ ID ID NO: signal BLASTE NO. Top Hit Descriptor NO: NO: Source Value 4675 9660 14643 0.96 4.0E-53 7705414 NT Homo saplens hook1 protein (HOOK10, mRNA Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 1/2 (DLEC1, ORCTL3, ORCTL4 genes, 2589 7552 12667 1. 47 3. 0E-53 AB026898. 1 NT complete cds) 3647 8653 13659 1. 94 3. OE-53 AW050836. 1 EST HUMAN wz22c07. x1 Soares Dieckgraefe colon NHCD Homo sapiens cDNA clone IMAGE : 2558796 3' 4457 9447 14427 0. 73 3. 0E-53 AW803563. 1 EST HUMAN IL2-UM0081-240300-055-D03 UM0081 Homo sapiens cDNA 455 5492 3. 58 2. 0E-53 AA366556. 1 EST HUMAN EST77525 Pancreas tumor III Homo sapiens cDNA 5'end Homo sapiens Bruton's tyrosine kinase (BTK), alpha-D-galactosidase A (GLA), L44-like ribosomal protein 2266 7243 12380 20. 13 2. 0E-53 U78027. 1 NT (L44L) and FTP3 (FTP3) genes, complete cds Homo sapiens ATPase, H+ transporting, iysosoma) 13355 1.23 1.0E-53 AB026898. proton pump) 31 kD ; Vacuolar proton-ATPase, 2466 0E-54 2. salens cDNA clone IMAGE E ; V-ATPase, subunit E (ATP6E), mRNA 3147 8163 13183 0. 92 2. 0E-53 7705687 NT Homo sapiens leucine aminopeptidase (LOC51056), mRNA 3172 8188 13210 2. 53 2. 0E-53 AF083822.1 NT Homo sapiens dihydropyridine receptor alpha 2 subunit (CACNA2D1) gene, exon 6 3947 8945 13935 2. 5 2. 0E-53 M61873. 1 NT Human Krueppel-related DNA-binding protein (TF34) gene, partial cds 4365 9356 14336 0. 92 2. 0E-53 4506962 NT Homo sapiens SKAP55 homologue (SKAP-HOM) mRNA 1420 6417 11477 1. 56 1. OE-53 AJ271736. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 2/2 Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 1/2 (DLEC1, ORCTL3, ORCTL4 genes, 3325 8335 13355 1.23 1.0E-53 AB026898. 1 NT complete cds) 4807 9791 14773 1. 54 1. OE-53 BE296386. 1 EST HUMAN 601176725F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 3531919 5' 5039 10010 14979 0. 97 1. OE-53 AW957429. 1 EST HUMAN EST369619 MAGE resequences, MAGE Homo sapiens cDNA 207 5271 10283 4. 09 8. 0E-54 BE386785. 1 EST HUMAN 601272863F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3614031 5' 1799 6790 11880 2. 71 8. 0E-54 4504610 NT Homo sapiens insulin-likegrowthfactor 2receptor (1GF2R) mRNA 4596 9584 14573 0. 71 8. 0E-54 4507848 NT Homo sapiens ubiquitin specific protease 13 (isopeptidase T-3) (USP13) mRNA 4596 9584 14574 0. 71 8. 0E-54 4507848 NT Homo sapiens ubiquitin specific protease 13 (isopeptidase T-3) (USP13) mRNA ai79c12. s1 Soares testis NHT Homo sapiens cDNA clone 1377046 3'similar to contains MER30. t3 MER30 383 5458 10475 1. 58 7. 0E-54 AA812537. 1 EST HUMAN repetitive element ; 1794 6785 11875 1. 37 7. 0E-54 Y16645. 1 NT Homo sapiens mRNA for monocyte chemotactic protein-2 yw68d12. s1 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA clone IMAGE : 257399 3' 2142 7121 12237 4. 24 7. 0E-54 N27177. 1 EST HUMAN similar to contains LTR7. b3 LTR7 repetitive element ; 5043 10014 14983 1.08 7.0e-54 A1276750. 1 EST_HUMAN ql64e10.x1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE : 1877130 3' 23 5103 10088 5. 96 6. 0E-54 AB003618. 1 NT Homo sapiens DNA for MICB, exon 4, 5 and partial cds 384 5459 10476 1. 73 6. OE-54 8922148 NT Homo sapiens hypothetical protein DKFZp434M035 (DKFZp434M035), mRNA 384 5459 10477 1. 73 6. OE-54 8922148 NT Homo sapiens hypothetical protein DKFZp434M035 (DKFZp434M035), mRNA 3210 8225 13247 2. 1 6. 0E-54 8922148 NT Homo sapiens hypothetical protein DKFZp434M035 (DKFZp434M035), mRNA 3898 8898 13896 1. 11 6. 0E-54 4502872 NT Homo sapiens chloride channel 6 (CLCN6) mRNA Page 109 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLASTE No. Dalsbase Top Hit Descriptor Value 4696 9681 14664 1. 19 6. 0E-54 4505806 NT Homo sapiens phosphatidylinostio 4-kinase, catalytic, alpha polypeptide (PIK4CA) mRNA 4724 9709 2. 36 6. 0E-54 Y09846. 1 NT H. sapiens shc pseudogene, p66 isoform 4841 9709 2. 18 6. 0E-54 Y09846. 1 NT H. sapiens shc pseudogene, p66 isoform 2090 7071 12185 3. 25 5. 0E-54 P51523 SWISSPROT ZINC FINGER PROTEIN 84 (ZINC FINGER PROTEIN HPF2) 182 5245 263. 62 4. 0E-54 AF110103. 1 NT Tupaia belangeri beta-acfln MRNA, partial cds EST177696 Jurkat t-cellsw VI Homo sapiens cDNA 5'end similar to glyceraldehyde-3-phosphate 941 5958 10991 140. 55 4. 0E-54 AA306764. 1 EST HUMAN dehydrogenase 1769 6761 11848 2. 55 4. 0E-54 D38521. 1 NT Human mRNA for K) AA0077 gene, partiat cds 1769 6761 11849 2. 55 4. OE-54 D38521. 1 NT Human mRNA for KIAA0077 gene, partial cds wd26d11. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2329269 3'similar to TR : 002711 3131 8147 1. 39 4. 0E-54 AI935086. 1 EST HUMAN 002711 PRO-POL-DUTPASE POLYPROTEIN ; 92 5169 10179 30. 76 3. 0E-54 AA313487. 1 EST HUMAN _ | carcinoma (HCC) cell line Homo sapiens cDNA 5'end 2551 7516 1. 04 3. 0E-54 AI908757. 1 ESTHUMAN L-BT189-190399-007 BT189 Homo sapiens cDNA 636 5664 10668 6. 13 2. 0E-54 5031900 NT Homo sapiens killer cell lectin-like receptor subfamily G, member 1 (KLRG1), mRNA 1347 6344 11395 2. 11 2. 0E-54 4507164 NT Homo sapiens nuclear antigen Sp100 (SP100) mRNA nt78a09.s1 NCI_CGAP_Pr3 Homo sapiens cDNA clone IMAGE : 1204600 similar to contains element L1 1515 6513 11569 1. 19 2. 0E-54 AA655008. 1 EST HUMAN repetitive element ; au92g03. y1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2783764 5'similar to 2469 7437 12554 1. 47 2. 0E-54 AW163175. 1 EST HUMAN SW : CUL 1_HUMAn Q13616 CULLIN HOMOLOG 1 ; 2524 7490 12610 1. 65 2. 0E-54 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21 C010 wy60b12. x1 SoaresNSFF89WOTPAPS1 Homo sapiens cDNA clone IMAGE : 2552927 3'similar to 2824 7845 12865 1. 51 2. 0E-54 AW057524. 1 EST HUMAN TR : Q62084 Q62084 PHOSPHOLIPASE C NEIGHBORING ; nj45g09. s1 NC) CGAPPr9 Homo sapiens cDNA clone IMAGE : 995488 similar to gb : X53777 60S 3472 8480 7. 32 2. 0E-54 AA532925. 1 EST HUMAN RIBOSOMAL PROTEIN L23 (HUMAN) ; 4082 9076 3. 11 2. 0E-54 4502642 NT Homo sapiens chaperonin containing T-complex subunit 6 (CCT6) mRNA 4319 9311 1.14 2.0E-54 AF206161.1 NT Homo sapiens syncylin precursor, mRNA, complele cds 5052 10023 14992 0. 92 2. 0E-54 4506962 NT Homo sapiens SKAP55 homologue (SKAP-HOM) mRNA 4341 9332 1. 35 1. OE-54 BF315418. 1 EST_HUMAN 601899230F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 4128535 5' 1298 6296 1. 8 8. OE-55 Y07829. 2 NT Homo sapiens RFB30 gene for RING finger protein yf26e04. r1 Scares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 127998 5'similar to 1065 6073 11106 1. 85 7. OE-55 R09346. 1 EST HUMAN SP : C561_BOVIN P10897 CYTOCHROME ; 1733 6728 11804 2. 5 5. 0E-55 AA704971. 1 EST_HUMAN zj95b09.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 462617 3' 1733 6728 11805 25 5.0E-55 AA704971.1 EST_HUMAN zj95b09.s1 Soares_fetal_Liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 462617 3' 4626 9611 14600 1. 31 5. OE-55 AW206021. 1 EST HUMAN UI-H-BI1-afy-g-09-0-Ul. s1 NCI CGAP Sub3 Homo sapiens cDNA clone IMAGE:2723536 3' Page 110 of 209 Tabie 4 Single Exon Probes Expressed in HBL100 Celis Probe Exon Most Simlar ORF SEQ Expression (Top) Hit top Hig Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLASt E No. Database Top Hit Descriptor Value 56 7712 10134 6. 49 4. OE-55 AW957994. 1 EST_HUMAN EST370064 MAGE resequences, MAGE Homo sapiens cDNA 664 5690 10699 29.4 4.0E-55 4826973 NT Homo sapiens RNA binding motif protein, Y chromosome, family 1, member A1 (RBMY1A1) mRNA 1414 6412 11471 1. 58 4. 0E-55 7661713 NT Homo sapiens predicted osteoblast protein (GS3786), mRNA 1414 6412 11472 1. 58 4. OE-55 7661713 NT Homo sapiens predicted osteoblast protein (GS3786), mRNA 7j52b10. x1 Soeres_NSF_f8_9W_OT_PA_P_S1 Homo sapiens cDNA clone IMAGE : 3390043 3'similar to 1483 6480 1. 43 4. OE-55 BF061411. 1 EST HUMAN contains L1. t3 L1 repetitive element ; 1974 6959 12064 1. 95 4. OE-55 4506180 NT Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 2 (PSMA2) mRNA 1974 6959 12065 1. 95 4. OE-55 4506180 NT Homo sapiens proteasome (prosome, macropain) subunit, alpha type, 2 (PSMA2) mRNA 2032 7015 12123 3. 3 4. OE-55 4503314 NT Homo sapiens diacyiglycerol kinase, gamma (90kD) (DGKG) mRNA 2032 7015 12124 3.3 4.0E-55 4503314 NT Homo sapiens diacyiglyceroi kinase, gamma (90kd) (DGKG) mRNA 2247 7224 12344 4. 51 4. OE-55 4507794 NT Homo sapiens ublqultin-conjugating enzyme E2 variant 1 (UBE2V1) mRNA 3206 8221 13243 1. 2 4. OE-55 AL163300. 2 NT Homo sapiens chromosome 21 segment HS21 C10 49859959 149362. 084. 0E-55 BE698671. 1 ESTHUMAN RC2-UT0023-290700-011-f03UT0023 Homo sapiens cDNA 376 5423 10438 2 2. OE-55 X57147. 1 NT Human endogenous retrovirus pHE.1 (ERV9) 547 5581 1. 08 2.0E-55 M10976. 1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 641 5669 10673 4. 78 2. OE-55 4507296 NT Homo sapiens syntaxin-binding protein 1 (STXBP1) mRNA, and translated products Homo sapiens ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angelman 2889 7908 12929 1. 7 2. 0E-55 4507798 NT syndrome) (UBE3A) mRNA 4629 9614 14604 2. 97 2. OE-55 BE719986. 1 EST_HUMAN CM1-HT0876-150800-357-g03 HT0876 Homo sapiens cDNA 95 61272 10182 2.86 1.0E-55 4505060 NT Homo sapiens mannose-8-phosphate receptor (calion de[emdemt_ (m6PR) mRNA 189 5253 10264 113.02 1.0E-55 U09823.1 NT Oryetolagus cunl;culus New Zealand white elongation factor 1 alpha (Rabefla2) mRNa, complele cds 569 5602 10600 14. 24 1.0E-55IA1026718. 1 EST_HUMAN ov85g09.x1 Soeres_Testis_NHT Homo sapiens cDNA clone IMAGE : 1644160 3' 1132 6137 11168 6. 88 1. OE-55 AB020710. 1 NT Homo sapiens mRNA for KIAA0903 protein, partial cds 1911 6897 11991 5 1.0E-55 BE277861. 1 EST HUMAN 601120116F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 2967027 5' 1911 6897 11992 5 1.0E-55 BE2767861. 1 EST HUMAN 601120116F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 2967027 5' 2263 7240 6. 39 1. OE-55 5803174 NT Homo sapiens SMA3 (SMA3), mRNA 2446 7416 12531 63. 73 I. OE-55 X13111. 1 NT Human mRNA for HLA-A11 E, a MHC class I molecule (major histocompatibility complex) 2483 7451 12564 3. 06 1. OE-55 AB007866. 2 NT Homo sapiens mRNA for KIAA0406 protein, partial cds 2483 7451 12565 3.06 1.0E-55 AB007866. 2 NT Homo sapiens mRNA for KIAA0406 protein, partial cds 2538 7503 12622 14.65 1.0E-55 L54057. 1 NT Homo sapiens CLP mRNA, partial cds 2717 7674 12787 0. 98 1. OE-55 AB033045. 1 NT Homo sapiens mRNA for KIAA1219 protein, partial cds Homo sapiens 5, 10-methylenetetrahydrofdate dehydrogenase, 5, 10-methylenetetrahydrofolate 3389 8397 13422 1.83 1.0E-55 5174500 NT cycvlohydrolase, 10-formyltatrahydrofolate synthetase (MTHFD) mRNA Page 111 of 209 Table 4 Single Fxnn Prahes Exnressed in HBI 100 cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLASTE No. Source Top Hit Descriptor Value Homo sapiens 5,10-methylenetetrahydrofolate dehydrogenase, 5, 10-methylenstetrahydrofolate 3389 8397 13423 1. 83 1. OE-55 5174590 NT cyciohydrolase, 10-formyltetrahydrofdate synthetase (MTHFD) mRNA 3883 8884 13882 3. 86 1. OE-55 AL163267. 2 NT Homo sapiens chromosome 21 segment HS21C067 4170 9165 14152 1.64 1.0E-55 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 4592 9580 1. 01 1. OE-55 N77261. 1 EST HUMAN yv44g03. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 245620 5' 4958 9935 14913 1. 081. 0E-55 8923125 NT Homo sapiens hypothetical protein FLJ20126 (FLJ20126), mRNA 5015 9986 14962 2. 2 1. OE-55 BE077198. 1 EST HUMAN RC5-BT0605-150200-031-B11 BT0605 Homo sapiens cDNA yn62g03. r1 Soares adult brain N2b5HB55Y Homo sapiens cDNA clone IMAGE : 173044 5' similar to contains 2659 7619 12730 3. 83 7. OE-56 H19934. 1 EST HUMAN THR repetitive element ; 1653 6649 11721 1. 83 5. OE-56 AW997712. 1 EST_HUMAN RC3-BN0053-170200-0011-h01 BN0053 Homo sapiens cDNA 28 5108 10092 44. 14 4.0E-56 AF141349. 1 NT Homo sapiens beta-tubulin mRNA, complete cds 28 5108 10093 44. 14 4. OE-56 AF141349. 1 NT Homo sapiens beta-tubulin mRNA, complete cds 2636 7596 12709 7. 3 4. OE-56 4507728 NT Homo sapiens tubulin, beta polypeptide (TUBB) mRNA 2636 7596 12710 7.3 4.0E-56 457728 NT Homo sapiens tubulin, beta polypeptide (TUBB) mRNA Homo sapiens X-linked anhidroitic ectodermal dyspiasia protein gene (EDA), exon 2 and flanking repeat 2740 5557 10560 3. 56 4.0E-56 AF003528. 1 NT regions 1322 6320 11368 3. 78 3. OE-56 8924029 NT Homo sapiens hypothetical protein PRO1304 (PRO1304), mRNA 1727 6722 11800 3. 22 3.0E-56 6912743 NT Homo sapiens 5'-3'exoribonuclease 2 (XRN2), mRNA 2089 7070 12184 1. 19 3. OE-56 6912697 NT Homo sapiens oncogene TC21 (TC21), mRNA 3052 8069 13.78 1. 47 3. OE-56 AA325826. 1 EST HUMAN EST28889 Cerebellum II Homo sapiens cDNA 5'end 3052 8069 13079 1. 47 3. OE-56 AA325826. 1 EST HUMAN EST28889 Cerebellum II Homo sapiens cDNA 5'end 3745 8749 2.03 3.0E-56 AF055066. 1 NT Homo sapiens MHC class 1 region 3818 8820 13827 1.28 3.0E-56 BE393512. 1 ES%T_HUMAN 601310203F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3631848 5' 4263 9256 14246 1. 01 3. OE-56 7657042 NT Homo sapiens Down syndrome candidate region 1 (DSCR1), mRNA 4300 9292 14279 4. 75 3. OE-56 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21 C068 4440 9430 14414 2. 24 3.0E-56 5902085 NT Homo sapiens superkiller viralicldic activity 2 (S. cerevisiae homoiog)-like (SKIV2L), mRNA 4941 9918 14898 0. 72 3.0e-56 6912593 NT Homo sapiens phosphotidylinosito transfer protein, beta (PITPNB), mRNA 4984 9918 14898 0. 83 3. OE-56 6912593 NT Homo sapiens phosphotidyiinositol transfer protein, beta (PITPNB), mRNA 520 5555 1. 87 2. OE-56 AA199818. 1 ESTHUMAN zq52a08. s1 Stratagene neuroepithelium (#937231) Homo sapiens cDNA clone IMAGE : 645206 3' 723 7729 10765 1. 67 2.0E-56 BE064386. 1 EST HUMAN RC4-BT0310-110300-015-f10 BT0310 Homo sapiens cDNA 723 7729 10766 1. 67 2. OE-56 BE064386. 1 EST HUMAN RC4-BT0310-110300-015-f10 BT0310 Homo sapiens cDNA 23217296 12416 0. 94 2.0E-56 M260-61. 1 NT Human cGMP phosphodiesterase alpha subunit (CGPR-A) mRNA, complete cds 2321 7296 12417 0. 94 2.0E-56 M26061.1 NT Human cGMP phosphodiesterase alpha subunit (CGPR-A) mRNA, complete cds 2919 7938 12955 1.04 2.0E-56 AB037835.1 NT Homo sapiens mRNA for KIAA1414 protein, partial cds Page 112 of 209 Table 4 Sinqle Exort Probes Exaressed irt H8L100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor NOI: t NO : Signa) BLAST E No. Source Value 3242 8255 1. 89 2. 0E-56 AB008681. 1 NT Homo sapiens gene for activin receptor type ! ! B, complete cds 3456 8464 13491 1. 29 2. OE-56 AV703184. 1 EST-HUMAN AV703184 ADB Homo sapiens cDNA clone ADBCFG10 5' 965 5980 4. 42 1. 0E-56 AF190930.1 NT Macaca fascicularis protein tyrosine phosphatase (PRL-1) mRNA, complete cds zt54b09. r1 Soares ovary tumor NbHOT Homo sapiens cDNA clone IMAGE : 726137 5'similar to gb : M94654 1489 6486 11541 2. 19 1. 0E-56IAA293036. 1 EST_HUMAN INTERLEUKIN ENHANCER-BINDING FACTOR (HUMAN) ; 3590 8597 13601 2.26 1.0E-56 AW589833.1 EST_HUMAN hg23c11.x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2946452 3' 3590 8597 13602 2. 26 1. 0E-56 AW589833. 1 EST_HUMAN hg23c11.x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2946452 3' 620 5647 1. 82 9. 0E-57 AW880885. 1. EST HUMAN QVO-OT0033-070300-152-h03 OT0033 Homo sapiens cDNA 4081 9075 14063 0. 97 9. 0E-57 4758279 NT Homo sapiens EphA4 (EPHA4) mRNA 4081 9075 14064 0. 97 9. 0E-57 4758279 NT Homo sapiens EphA4 (EPHA4) mRNA 295 5352 10365 2. 81 8. 0E-57 AW816405. 1 EST HUMAN QV4-ST0234-181199-037-f05 ST0234 Homo sapiens cDNA xr05d10. x1 NCI_CGAP_Brn53 Homo sapiens cDNA clone IMAGE : 2759251 3'similar to gb : U05875 873 5891 10932 7. 02 8. 0E-57 AW264599. 1 EST_HUMAN INTERFERON_GAMMA RECEPTOR BETA CHAIN PRECURSOR (HUMAN) ; 1780 6772 11864 1. 69 8. 0E-57 AA496109. 1 EST HUMAN zv51b12. r1 Soares tests NHT Homo sapiens cDNA clone IMAGE : 757151 5' 3297 8308 13333 1. 37 8. 0E-57 4758279 NT Homo sapiens EphA4 (EPHA4) mRNA 3297 8308 13334 1. 37 8. 0E5-7 4758279 NT Homo sapiens EphA4 (EPHA4) mRNA 4869 9848 14825 0. 81 8. 0E-57 BE299916. 1 EST_HUMAN 600944440F1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE : 2960864 5' 2561 7525 12642 0. 97 7. 0E-57 7657592 NT Homo sapiens smg GDS-ASSOCIATED PROTEIN (SMAP), mRNA I 2561 7525 12643 0. 97 7. OE-57 7657592 NT Homo sapiens smg GDS-ASSOCIATED PROTEIN (SMAP), mRNA I 3177 8193 13214 1. 16 7. OE-57 7242158 NT Homo sapiens NME7 (NME7), mRNA 3177 8193 13215 1. 16 7. 0E-57 7242158 NT Homo sapiens NME7 (NME7), mRNA 3197 8213 13235 0. 74 7. 0E-57 6005979 NT Homo sapiens Kruppel-like factor 8 (KLF8), mRNA 3786 8789 13793 2. 1 7. OE-57 AF012872. 1 NT Homo sapiens phosphatidyfinostto) 4-kinase 230 (pi4K230) mRNA, complete cds 3786 8789 13794 2.1 7.0E-57 AF012872.1 NT Homo sapiens phosphatidyinositol 4-kinase 230 (plk4K230) mRNA, compl;ete cds 4308 9300 1. 78 7. 0E-57 AF020503. 1 NT Homo sapiens FRA3B common fragile region, diadenosine triphosphate hydrolase (FHIT) gene, exon 5 Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 1/2 (DLEC1, ORCTL3, ORCTL4 genes, 3668 8673 13677 2. 42 4. 0E-57 AB026898. 1 NT complete cds) Homo sapiens ubiquitin protein ligase E3A (human papilloma virus E6-associated protein, Angel;man 795 5816 10847 1. 52 3. 0E-57 4507798 NT syndrome) (UBE3A) mRNA nc13fO7. s1 NCl_CGAP_Pr1 Homo sapiens cDNA clone IMAGE : 1008037 similar to SW : RS10HUMAN 1311 6308 153. 33 3. 0E-57 AA230279. 1 EST HUMAN P46783 40S RIBOSOMAL PROTEIN S10. ; 2327 7301 124211. 31 3. 0E-57 AA348335. 1 EST HUMAN EST54770 Hippocampus II Homo sapiens cDNA 5'end Page 113 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORF SEQ Expresslon (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: NO: Source Value 7f33b10.x1 NCI_CGAP_CLL1 HOmo sampiens cDNA clone IMAGE:3296443 3' simllar to WP:Y47H9C.2 2631 7591 12703 1. 45 3. 0E-57 BE676622. 1 EST HUMAN CE20263 ; 7f33b10. x1 NCI_CGAP_CLL1 Homo sapiens cDNA clone IMAGE : 3296443 3'similar to WP : Y47H9C. 2 2631 7591 12704 1. 45 3. OE-57 BE676622. 1 EST HUMAN CE20263 ; 3482 8490 13506 1. 73 3. 0E-57 AF232708. 1 NT Homo sapiens cell-line tsA201 a chloride ion current inducer protein I (Cln) gene, complete cds 3614 8621 31. 12 3. OE-57 AW853964. 1 EST HUMAN RC3-CT0254-110300-027-d10 CT0254 Homo sapiens cDNA 1471 6468 11526 1. 55 2. 0E-57 AF246219. 1 NT Homo sapiens SNARE protein kinase SNAK mRNA, complete cds 1471 6468 11527 1. 55 2. OE-57 AF246219. 1 NT Homo sapiens SNARE protein kinase SNAK mRNA, complete cds 2337 7311 12432 1. 04 2. 0E-57 BE172526. 1 ESTHUMAN MRO-HT0559-010400-009-h10HT0559 Homo sapiens cDNA 3354 8362 3. 52 2. 0E-57 AL163204. 2 NT Homo sapiens chromosome 21 segment HS21 C004 3474 8482 13501 0. 68 2. 0E-57 R07702. 1 EST_HUMAN ye98h01.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 125809 5' 3474 8482 13502 0. 68 2. 0E-57 R07702.1 EST_HUMAN ye98h01.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 125809 5' 3828 8830 13837 0. 83 2. 0E-57 BE073264. 1 ESTHUMAN MRO-BT0551-060300-103-b03BT0551 Homo sapiens cDNA 4063 9057 14044 1. 05 2. 0E-57 AA018299. 1 ESTHUMAN ze40c06. r1 Scares retina N2b4HR Homo sapiens cDNA clone IMAGE : 361450 5' 4063 9057 14045 1. 05 2. 0E-57 AA018299. 1 EST_HUMAN ze40cO6. r1 Soares retina N2b4HR Homo sapiens cDNA clone IMAGE : 361450 5' 4379 9370 14349 8. 09 2. 0E-57 AL163283. 2 NT Homo sapiens chromosome 21 segment HS21 C083 2170 7149 12266 1. 48 1. OE-57 AW503208. 1 EST_HUMAN UI-HF-BN0-akt-g-07-0-UI.r1 NIH_MGC_50 Homo sapiens cDNA clone IMAGE : 3078348 5' 584 5615 1. 9 8. OE-58 BE868715. 1 EST-HUMAN 601445948F1 NIH_MGC_^5 Homo sapiens cDNA clone IMAGE : 3850211 5' tr534b07. x1 NCI_CGAP_Ov23 Homo sapiens cDNA clone IMAGE : 2220181 3'similar to TR : 015475 015475 646 5674 10679 4. 07 8. 0E-58 AI798376. 1 EST HUMAN UNNAMED HERV-H PROTEIN ; tr34b07. x1 NCI_CGAP_Ov23 Homo sapiens cDNA clone IMAGE : 2220181 3'similar to TR : 015475 015475 646 5674 10680 4. 07 8. OE-58 AI798376. 1 EST HUMAN UNNAMED HERV-H PROTEIN ; 1820 6810 11902 1. 74 8. OE-58 11434921 NT Homo sapiens putative protein O-mannosyttransferase (POMT2), mRNA 1820 6810 11903 1. 74 8. OE-58 11434921 NT Homo sapiens putative protein O-mannosyltransferase (POMT2), mRNA 2906 7925 2. 79 8. OE-58 7706132 NT Homo sapiens DHHC1 protein (LOC51304), mRNA 2192 7171 12292 0. 98 6. OE-58 BE395061. 1 EST_HUMAN 601309465F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3631000 5' 2314 7289 12410 10. 37 6. 0E-58 AU130689.1 EST_HUMAN AU130689 NT2RP3 Homo sapiens cDNA clone NT2RP3001263 5' TCAAP1E1219 Pediatric acute myelogenous leukemia cell (FAB M1) Bayior-HGSC project=TCAA Homo 2831 7851 12870 0. 94 6. 0E-58 BE242150. 1 EST HUMAN sapiens cDNA clone TCAAP1219 TCAAP1E1219 Pediatric acute myelogenous leukemia cell (FAB M1) Baylor-HGSC project=TCAA Homo 2831 7851 12871 0. 94 6. OE-58 BE242150. 1 EST HUMAN sapiens cDNA clone TCAAP1219 298 5355 10367 3. 24 5. 0E-58 4507334 NT Homo sapiens synaptojanin 1 (SYNJ1), mRNA 700 5724 10740 6.5 5.0E-58 BE763984. 1 ESTHUMAN RC4-NT0057-160600-016-b05NT0057 Homo sapiens cDNA 1175 6178 11213 3. 64 5. OE-58 AW797948. 1 EST HUMAN CM3-UM0043-240300-127-e07 UM0043 Homo sapiens cDNA Page 114 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID ID NO: @@@@@@ @@ @@@@ @@ Database Top Hit Descriptor Value Source Value 1175 6178 11214 3.64 5.0e-58 AW797948.1 EST_HUMAN CM3-UM0043-240300-127-e07 UM0043 Homo sapiens cDNA 1176 6178 11213 2. 81 5. 0E-58 AW797948. 1 EST HUMAN CM3-UM0043-240300-127-e07 UM0043 Homo sapiens cDNA 1176 6178 11214 2. 81 5. 0E-58 AW797948. 1 ESTHUMAN CM3-UM0043-240300-127-e07UM0043 Homo sapiens cDNA 3249 8262 13283 9. 15 5. 0E-58 AA988183. 1 EST_HUMAN or98e07.s1 NCI_CGAP_Lu5 Homo sapiens cDNA clone IMAGE : 1603908 3' ts89e07. x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2238468 3'similar to SW : PR02ACACA 4136 9131 14114 0. 98 5. 0E-58 AI636745. 1 EST_HUMAN P19984 PrOFILIN II ; Homo sapiens ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit (oligomycin sensitivity I 372 5421 10435 8. 26 4. 0E-58 4502302 NT conferring protein) (ATP50) mRNA 788 5809 10839 1. 73 4. 0E-58 4504634 NT Homo sapiens interleukin 10 receptor, beta (IL10RB), mRNA Homo sapiens coagulation factor IX (plasma thromboplastic component ; Christmas disease, hemophilia B) 1440 6437 11494 1. 06 4. 0E-58 4503648 NT (F9) mRNA 2559 7524 12640 2.19 4.0E-58 U36251.1 NT Human beta-prime adaptin (BAM22) gene, exon 3 3655 8661 13666 1. 09 4. OE-58 5031660 NT Homo sapiens EGF-like repeats and discoidin I-like domains 3 (EDIL3), mRNA 333 5385 1. 23 3. OE-58 R17879. 1 EST HUMAN yg10e02. r1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 31693 5' 1366 6363 11412 1. 98 3. 0E-58 4758981 NT Homo sapiens peptide YY (PYY) mRNA 3105 8121 13138 3. 33 3. 0E-58 BF569848. 1 EST_HUMAN 602185789F1 NIH_MGC_45 Homo sapiens cDNA clone IMAGE : 4309943 5' 3105 8121 13139 3. 33 3. 0E-58 BF569848. 1 EST_HUMAN 602185789F1 NIH_MGC_45 Homo sapiens cDNA clone IMAGE : 4309943 5' 926 5943 10977 7. 87 2. OE-58 AF068624. 1 NT Homo sapiens 5-aminolevulinate synthase 2 (ALAS2) gene, complete cds ba08b07. y1 NIH_MGC_7 Homo sapiens cDNA clone IMAGE : 2823733 5'similar to gb : X69391 60S RIBOSOMAL PROTEIN L6 (HUMAN) ; gb : X81987 M. musculus mRNA for TAX responsive element binding 1271 6269 27. 01 2. 0E-58 BE208532. 1 EST HUMAN protein (MOUSE) ; 712 5736 10752 0. 84 1. OE-58 M65134. 1 NT Human complement component C5 mRNA, 3'end 1051 6060 11089 9. 81 1. 0E-58 6274549 NT Homo sapiens NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (22kD, B22) (NDUFB9), mRNA 1307 6305 11352 1. 04 1. OE-58 AW957182. 1 EST-HUMAN EST369252 MAGE resequences, MAGD Homo sapiens cDNA 1307 6305 11353 1. 04 1. 0E-58 AW957182. 1 EST HUMAN EST369252 MAGE resequences, MAGD Homo sapiens cDNA 1373 6370 11419 3. 35 1. 0E-58 AJ238093. 1 NT Homo sapiens partial AF-4 gene, exons 2 to 7 and Alu repeat elements 1624 6621 11689 1. 46 1. OE-58 BE466132. 1 EST_HUMAN hy10f08.x1 NCI_CGAP__GC6 Homo sapiens cDNA clone IMAGE : 3196935 3' 2582 7545 12659 0. 96 1. OE-58 AF217514. 1 NT Homo sapiens uncharacterized bone marrow protein BM038 mRNA, complete cds 2730 7687 12801 1. 98 1. OE-58 4759169 NT Homo sapiens sterol regulatory element binding transcription factor 2 (SREBF2) mRNA 4811 9795 14778 5. 66 1. 0E-58 AI141063. 1 ESTHUMAN oz43h01. x1 SoaresNhHMPuS1 Homo sapiens cDNA clone IMAGE : 1678129 3' 2168 7147 12264 69.17 8. 0E-59 4507378 NT Homo sapiens TATA box binding protein (TBP) mRNA 177 7714 2. 96 6. 0E-59 BF035327.; 1 EST_HUMAN 601458531F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3862086 5' Page 115 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Slmllar Probe Exon top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID ID NO: Signal BLAST E No. source NO: NO: source Value au93h05.x1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE:27838665 3' simllar to 1715 6710 11786 9.16 5.0e-59 AW157281.1 EST_HUMAN TR:O75785 075786 GANGLIOSIDE-INDUcED DIFFERENTIATION ASSOCIATED PROTEIN 1.; au93h05.x1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE:2783865 3' slmllar to au93h05.x1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2783865 3'similar to 1715 6710 11787 9. 16 5. 0E-59 AW 157281. 1 EST_HUMAN TR : 075786 075786 GANGLIOSIDE-INDUCED DIFFERENTIATION ASSOCIATED PROTEIN 1. ; 3054 8071 13081 6. 86 5. 0E-59 AI807484. 1 ESTHUMAN wf48c11. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2358836 3' 4523 9513 14498 9. 33 5. OE-59 X83497. 1 NT H. sapiens DNA for ZNF80-linked ERV9 long terminal repeat 785 5806 10837 2. 84 4. 0E-59 D80006. 1 NT Human mRNA for KIAA0184 gene, partial cds Homo sapiens phosphatidylinositol-4-phosphate 5-kinase, type II, beta (PIP5K2B) mRNA, and translated 1218 6217 11258 0. 67 4. 0E-59 4505818 NT products Homo sapiens phosphatidylinositol-4-phosphate 5-kinase, type il, beta (PIP5K2B) mRNA, and translated 1218 6217 11259 0. 67 4. 0E-59 4505818 NT producte 5017 9988 14964 0. 96 4. 0E-59 AI990847. 1 EST HUMAN ws32e12. x1 NCI CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2498926 3' 10 5090 4. 74 3. 0E-59 AW965524. 1 EST HUMAN EST377582 MAGE resequences, MAGI Homo sapiens cDNA 225 5287 10295 4. 43 3. 0E-59 7662247 NT Homo sapiens KIAA0680 gene product (KIAA0680), mRNA 1672 6668 11743 8. 3 3. 0E-59 4505860 NT Homo sapiens plasminogen activator, tissue (PLATa) mRNA 1672 6668 11744 8. 3 3. 0E-59 4505860 NT Homo sapiens plasminogen activator, tissue (PLATa) mRNA 2071 7053 12162 5. 59 3. 0E-59 AB029035. 1 NT Homo sapiens mRNA for KIAA1112 protein, partial cds 2071 7053 12163 5. 59 3.0E-59 AB029035. 1 NT Homo sapiens mRNA for KIAA1112 protein, partial cds 2697 7779 12769 0.98 3. 0E-59 AF232299. 1 NT Homo sapiens NF1-2 pseudogene, exon 17 3056 8073 13085 3. 67 3. 0E-59 4502014 NT Homo sapiens A kinase (PRKA) anchor protein 1 (AKAP1), mRNA 3056 8073 13086 3. 67 3. 0E-59 4502014 NT Homo sapiens A kinase (PRKA) anchor protein 1 (AKAP1), mRNA 3735 8739 13738 1. 33 3. 0E-59 4508044 NT Homo sapiens zona pellucid glycoprotein 2 (sperm receptor) (ZP2) mRNA 4547 9536 14523 1. 09 3. 0E-59 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21C084 4693 9678 14662 1. 64 3. 0E-59 7427522 NT Homo sapiens protein tyrosine phosphatase, receptor type, T (PTPRT), mRNA 4884 9863 0.92 3.0E-59 m95961. 1 NT Human prohormone converting enzyme (NEC2) gene, exon 2 162 5228 37. 68 1. 0E-59 BE296411. 1 EST_HUMAN 601176757F1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE:3531927 5' ca56h11.s1 NCI_CGAP_GCB1 Homo sapiens cDNA clone IMAGE : 1309029 3'similar to TR : Q13537 2544 7509 2. 32 1. 0E-59 AA748468. 1 ESt_HUMAN Q13537 MER37 TRANSPOSABLE ELEMENT, COMPLETE CONSENSUS SEQUENCE. ; 754 5776 10803 2. 17 8. 0E-60 AW977845. 1 EST HUMAN EST389849 MAGE resequences, MAGO Homo sapiens cDNA 1443 6440 11497 8. 32 8. 0E-60 4759159 NT Homo sapiens small nuclear ribonucleoprotein D3 polypeptide (18kD) (SNRPD3) mRNA 2107 7087 12201 1. 59 8. 0E-60 5174656 NT Homo sapiens differentiation-related gene 1 (nickel-specific induction protein) (RTP) mRNA 2107 7087 12202, 1. 59 8. 0E-60 5174656 NT Homo sapiens differentiation-rotated gene 1 (nickel-specific induction protein) (RTP) mRNA 745 5768 10974 33.65 7.0E-60 AF055066.1 NT Homo sapiens MHC class 1 region 746 5768 10794 109. 11 7. OE-60 AF055066. 1 NIT Homo sapiens MHC class 1 region Page 116 of 209 Table 4 Sinale Exon Probes Exoressed in HRt nn rpn Probe Exon ORF SFQ Fxnression Most Similar Top Hit ORFSEQ Expression (Top) Hit Top Hit Acession Database Top Hit NO: NO: ID NO: Signal BLAST E No. Dauatbase top Hit Descriptor Source Value 805 5826 10856 1.3 7.0E060 4504534 NT Homo sapiens interleukin 10 recpeotr, beta (IL 10RB), mRNA 20707052121611. 237. 0E-60 AF077188. 1 NTHomo sapiens cullin 4A (CUL4A) mRNA, complete cds 2712 7669 12782 0. 98 7. 0E-60 AB011153. 1 NT Homo sapiens mRNA for KIAA0581 protein, partial cds 4055 9049 14037 4. 26 7. OE-60 4505488 NT Homo sapiens ornithine decarboxylase 1 (ODC1) mRNA 2114 7094 12208 1.15 6.0E-60 BE964974. 2 EST_HUMAN 601658751R1 NIH_MGC_69 Homo sapiens cDNA clone IMAGE : 3886069 3' 82 5159 10169 0. 96 5.0E-60 AI807917.1 EST_HUMAN wf52c07.x1 Soares_NFL. T GBC S1 Homo sapiens cDNA clone IMAGE : 23592123' 82 5159 10170 0. 96 5. 0E-60 Al807917. 1 EST_HUMAN wf52c07.x1 Soares_NFL_T GBC S1 Homo sapiens cDNA clone IMAGE : 2359212 3' 2173 7152 12271 1. 15 4. OE-60 AW503208. 1 EST_HUMAN UI-HF-BNO-akt-g-07-0-UI.r1 NIH MGC 50 Homo sapiens cDNA clone IMAGE : 3078348 5' 2173 7152 12272 1.15 4.0E-60 AW503208. 1 EST_HUMAN UI-HF_BN0-akt-g-07-0-Ul.r1 NIH_MGC_50 Homo saplens cDNA clone IMaGE : 3078348 5' 2903 7922 1. 51 4. OE-60 AA299037. 1 EST HUMAN EST11498 Uterus Homo sapiens cDNA 5'end similar to similar to retrovirus-related pol 1822 6812 11905 3.27 3.0E-60 BE562611, 1 EST_HUMAN 6013364456F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3690395 5' 1822 6812 11906 3.27 3.0E-60 BE662611. 1 EST HUMAN 601336446F1 NIH MGC 44 Homo sapiens cDNA clone IMAGE : 3690395 5' 1832 6822 9. 77 3. 0E-60 6031190 NT Homo sapiens prohibition (PHB) mRNA 43359326143111. 673. 0E-60 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1/2 31 5111 10097 1. 22 2.0E-60 AY008285. 1 NT Homo sapiens soute carrier (SLC25A18) mRNA, complete cds ; nuclear gene for mitochondrial product 1399 6396 11451 6. 79 2. OE-60 Z11694. 1 NT H. sapiens 41 kDa protein kinase related to rat ERK2 1683 6679 11752 1. 46 2. OE-60 M24603. 1 NT Human bcr protein mRNA, 5'end 1692 6688 11763 1. 24 2.0E-60 AY008285. 1 NT Homo sapiens solute carrier (SLC25A18) mRNA, complete cds ; nuclear gene for mitochondrial product 2532 7497 12617 1. 04 2. OE-60 7657229 NT Homo sapiens interleukin 17 receptor (IL1 7R), mRNA 3498 8506 13521 0. 82 2.0E-60 4757867 NT Homo sapiens v-raf murine sarcoma viral oncogene homolog B1 (BRAF) mRNA 3817 8819 13826 0.8 2.0E-60 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 518 5553 10556 1 1.0E-601BE178586. 1 EST HUMAN PM3-HT0605-270200-001-e06 HT0605 Homo sapiens cDNA 3806 8809 13815 1. 97 1. OE-60 AU143389. 1 EST HUMAN AU143389 Y79AA1 Homo sapiens cDNA clone Y79AA1001854 5 4799978314766 1. 67 1. OE-60 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 1082 6089 11118 2. 32 9.0E-61 AU119344. 1 EST HUMAN AU119344 HEMBA1 Homo sapiens cDNA clone HEMBA1005583 5' 2599 7561 12678 1. 72 8.0E-61 AW006478. 1 EST_HUMAN wt05b 10.x1 NCI_CGAP_Co3 Homo sapiens cDNA clone IMAGE : 2506555 3' 2599 7561 12679 1.72 8.0E-61 AW006478. 1 EST_HUMAN wt05b10.x1 NCI_CGAP_Co3 Homo sapiens cDNA clone IMAGE : 2506555 3' 2.34 8.0E-61 X57147. 1 NT Human endogenous retrovirus pHE. 1 (ERV9) i 128 5195 10209 1.8 7.0E-61 7706670 NT Homo sapiens PXR2b protein (PXR2b), mRNA 128 5195 10210 1.8 7.0E-61 7706670 NT Homo sapiens PXR2b protein (PXR2b), mRNA 4923 5195 10209 2. 92 7.0E-61 7706670 NT Homo sapiens PXR2b protein (PXR2b), mRNA 4923 5195 10210 2.92 7.0E-61 7706670 NT Homo sapiens PXR2b protein (PXR2b), mRNA Page 117 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most slmllar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database top Hit Descriptor ID NO: signal BLAST E No. NO : NO : Value Source 263 5322 10331 4. 42 6.0e-61 BE409310. 1 EST_HUMAN 601300938F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3635480 5' 802 5823 10853 2. 26 6. OE-61 BE409310. 1 ESTHUMAN 601300938F1NHMGC21 Homo sapiens cDNA clone IMAGE : 3635480 5' 1301 6299 11345 12. 89 6.0E-61 AF119860. 1 NT Homo sapiens PRO2014 mRNA, complete cds 1609 6605 11668 2.82 6.0E-61 AA596033. 1 EST_HUMAN nn66h09.s1 NCI_CGAP_Lar1 Homo sapiens cDNA clone IMAGE : 1088897 3' 2068 7050 12158 0.95 6.0E-61 AY008285. 1 NT Homo sapiens solute carrier (SLC25A18) mRNA, complete cds ; nuclear gene for mitochondrial product 3234 8249 13270 13.67 6.0E-61 AU130689. 1 EST HUMAN AU130689 NT2RP3 Homo sapiens cDNA clone NT2RP3001263 5' 358 5408 10420 0. 86 5. OE-61 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA 1639 6636 11706 3. 51 5.0E-61 4506008 NT Homo sapiens protein phosphatase 1, regulatory subunit 10 (PPP1 R10) mRNA 2965 7983 12997 2. 15 5. 0E-61 AL163279. 2 NT Homo sapiens chromosome 21 segment HS21 C079 3125 8141 13162 1. 7 5. 0E-61 450216 NT Homo sapiens amyloid beta (A4) precursor protein (protease nexin-ii, Alzheimer disease) (APP), mRNA 3874 8875 1.66 5.0E-61 AJ229041, 1 NT Homo sapiens 959 kb contig between AML1 and CBR1 on chromosome 21q22; segment 1/3 4843 5408 10420 1. 16 5. OE-61 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA 495 5531 10538 1.33 2.0E-61 8922829 NT Homo sapiens hypothetical protein FLJ11026 (FLJ11026), mRNA 1193 6194 11231 3. 21 2. 0E-61 BE168410. 1 EST HUMAN QV3-HT0513-060400-147-d01 HT0513 Homo sapiens cDNA 1193 6194 11232 3.21 2.0E-61 BE168410.1 EST_HUMAN QV3-HT0513-606400-147-d01 HT0513 Homo sapiens cDNA yv53d11. s1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 246453 3'similar to 1626 6623 11692 1.75 2.0E-61 N53039. 1EST-HUMAN gb : L25444 60S RIBOSOMAL PROTEIN L35A (HUMAN) ; 2570 7533 1. 36 2. OE-61 N39397. 1ESTHUMAN yy03f11. r1 Scares metanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 270189 5' 432 5470 0. 75 1. OE-61 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 764 5785 10814 1. 16 1. OE-61 5453829 NT Homo sapiens origin recognition complex, subunit 2 (yeast homolog)-like (ORC2L) mRNA 1732 6727 0. 96 1. OE-61 U32657. 1 NT Human polymorphic trinucleotide repeat in X-linked renitis pigmentosa (RP3) gene region 1821 6811 11904 4. 18 1. OE-61 6005983 NT Homo sapiens zona pellucida glycoprotein 3A (sperm receptor) (ZP3A), mRNA xn1 1b09.y1 NCI_CGAP_Li5 Homo sapiens cDNA clone IMAGE : 2693369 5'similar to contains element 2134 7114 12227 1.82 1.0e-61 AW827281. 1 EST HUMAN MSR1 repetitive element ; 2761 7782 12807 2. 42 1. OE-61 BE386363. 1 EST_HUMAN 601273513F1 NIH_MGC_20 Homo sapiens cDNA clone IMAGE : 3614667 5' 3291 8302 13328 0. 73 1. OE-61 7662319 NT Homo sapiens KIaA0806 gene product (KIAA0806), mRNA 4317 9309 14294 0. 75 1. OE-61 4759249 NT Homo sapiens TRAF family member-associated NFKB activator (TANK) mRNA 4317 9309 14295 0. 75 1.0E-61 4759249 NT Homo sapiens TRAF family member-associated NFKB activator (TANK) mRNA 4708 9693 14676 9. 13 1. OE-61 AW298181. 1 EST HUMAN Ul-H-BWO-ajt-b-08-0-Ul. s1 NCI CGAP Sub6 Homo sapiens cDNA clone IMAGE : 2732871 3' 4708 9693 14677 9.13 1.0E-61 AW298181. 1 EST HUMAN UI-H-BWO-ajt-b-08-0-Ul. s1 NCl_CGAP Sub6 Homo sapiens cDNA clone IMAGE : 2732871 3' oc66h11. s1 NCfCGAPGCB1 Homo sapiens cDNA clone IMAGE : 1354725 3'similar to SW : POL_MLVRK 4420 94 P31795 POL POLYPROTEIN ; Page 118 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit Probe Exon SEQ ID @SEQ ID OrF SEQ Expression (Top) Hit top Hit Acession RF SEQ EXpression (Top) HIt lop Hit Acession database top Hit Descriptor Value 1090 6097 11126 1. 09 7. OE-62 AV714334. 1 EST HUMAN AV714334 DCB Homo sapiens cDNA clone DCBAMA08 5' NUCLEOLAR TRANSCRIPTION FACTOR 1 (UPSTREAM BINDING FACTOR 1) (UBF-1) 3427 8435 13461 0. 93 7. OE-62 P17480 SWISSPROT (AUTOANTIGEN NOR-90) 2930 7949 1. 56 6. OE-62 U09410. 1 NT Human zinc finger protein ZNF131 mRNA, partial cds 3300 8311 4. 7 6. 0E-62 11418255 NT Homo sapiens CGI_56 protein (CGI-56), mRnA wx51e07. x1 NCtCGAPLu28 Homo sapiens cDNA clone IMAGE : 2547204 3'similar to SW : GG95_HUMAN 413 5450 10470 3. 65 5. OE-62 |AI950528. 1 EST HUMAN Q08379 GOL GIN-95.; contains element MER22 repetitive element ; 2341 7315 12435 3.43 5. OE-62 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1/2 2341 7315 124363. 43 5. 0E-62 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1/2 2515 7483 12599 0.98 5. OE-62 U39487. 1 NT Human xanthine dehydrogenase/oxidase mRNA, complete cds 2515 7483 12600 0. 98 5. OE-62 U39487. 1 NT Human xanthine dehydrogenase/oxidase mRNA, complete cds 3334 8344 13362 2.46 5.0e-62 4506758 NT Homo sapiens ryanodine receptor 3 (RYR3) mRNA zw78e09. s1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 782344 3'similar to SW : NRDC_RAT 4201 9194 14176 1. 82 5. OE-62 AA431093. 1 EST HUMAN P47245 NARDILYSIN ; 4427 9417 1. 12 5. OE-62 AW905887. 1 EST HUMAN RC5-NN1089-100500-021-H03 NN1089 Homo sapiens cDNA au71d03. y1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2781701 5'similar to gb : M37104 830 5850 10887 5. 18 4. 0E-62 AW161479. 1 EST HUMAN ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL PRECURSOR (HUMAN) ; au71d03. y1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2781701 5'similar to gb : M37104 830 5850 10888 5. 18 4. 0E-62 AW161479. 1 EST HUMAN ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL PRECURSOR (HUMAN) ; au71d03. y1 Schneider fetal brain 00004 Homo saplens cDNA clone IMAGE : 2781701 5'similartogb : M37104 831 5850 10887 3. 4. 0E-62 AW161479. 1 EST HUMAN ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL PRECURSOR (HUMAN) ; au71 d03.yl Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2781701 5'similar to gb : M37104 831 5850 10888 3. 4. 0E-62 AW161479. 1 EST HUMAN ATP SyNTHASE COUPLING FACTOR 6, MITOCHONDRIAL PRECURSOR (HUMAN) ; wf12b08. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2350359 3'similar to 2389 7360 12482 4. 4. 0E-62 AI627900. 1 EST HUMAN gb : X57138_rna1 HISTONE H2B.2 (HUMAN) ; wf12b08. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2350359 3'similar to 2389 7360 12483 4. 43 4. 0E-62 Al82790. 1 EST HUMAN gb : X57138_ma1 HISTONE H2B. 2 (HUMAN) ; 3315 832 7. 88 4. OE-62 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA 5023 9994 14967 0. 97 4. 0E-62 4758323 NT Homo sapiens enhancer of zeste (Drosophila) homolog 2 (EZH2) mRNA 73 5151 10160 1. 46 3. OE-62 4557794 NT Homo sapiens neurofibromin 2 (bilateral acoustic neuroma) (NF2) mRNA 2972 3. OE-62 AB040909. 1 NT Homo sapiens mRNA for Kit1476 protein, partial cds 2972 7990 13004 0.71 3.0E-62 AB040909.1 NT Homo sapiens mRNA for KIAA1476 protein, partial cds 3615 8622 13630 8. 12 3. OE-62 X52858. 1 NT Human cyclophilin-related processed pseudogene 1211 6211 11251 2,89 2.0E-62 AL163284.2 NT Homo sapiens chromosome 21 segment HS21C084 Page 119 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor Value 1027 6037 11069 1. 58 1. OE-62 AF248540. 1 NT Homosapiens intersecn 2 (SH3D1B) mRNA, completecds 1512 6510 11567 15. 3 1. OE-62 L78810. 1 NT Homo sapiens ADP/ATP carrier protein (ANT-2) gene, complete cds af70e11. r1 SoaresNhHMPuS1 Homo sapiens cDNA clone IMAGE : 1047404 5'similar to WP : K01H12. 1 1763 6755 11840 1. 92 1. OE-62 AA625207. 1 EST HUMAN CE03453 ; 2844 7864 12882 1.18 1.0e-62 AL039044. 1 EST HUMAN DKFZp566F104 r1 566 (synonym : hfkd2) Homo sapiens cDNA clone DKFZp566F104 5' 3339 8348 2. 49 1. OE-62 AB040911. 1 NT Homo sapiens mRNA for KIAA1478 protein, partial cds 4395 9386 14369 1. 631. OE-62 8923201 NT Homo sapiens hypothetical protein FLJ20212 (FLJ20212), mRNA ziO6bO8. r1 SoarespregnantuterusNbHPU Homo sapiens cDNA clone IMAGE : 491511 5'similar to 4956 9933 14911 0. 98 1. OE-62 AA148822. 1 EST HUMAN SW : C561_BOVIN P10897 CYTOCHROME B561.; 336 5388 10395 1.82 9.0E-63 AW816405. 1 ESt_HUMAN QV4-ST0234-181199-037-f05 ST0234 Homo sapiens cDNA 2284 7260 1. 099. 0E-63 C18159. 1 EST HUMAN C18159 Human placenta cDNA (TFujiwara) Homo sapiens cDNA clone GEN-558C10 5' 3928 8928 13918 9. 26 9.0E-63 AB002348. 2 NT Homo sapiens mRNA for KIAA0350 protein, partial cds 3928 8928 13919 9. 26 9.0E-63 AB002348. 2 NT Homo sapiens mRNA for KIAA0350 protein, partial cds ze31d08. r1 Scares retina N2b4HR Homo sapiens cDNA clone IMAGE : 360591 5'similar to 5056 10026 14995 4. 71 9. OE-63 AA015938. 1 EST HUMAN SW : UN13_CAEEL P27715 PHORBOL ESTER/DIACYLGLYCEROL-BINDING PROTEIN UNC-13. [1] ; 2282 7258 12376 2. 39 8. 0E-63 4557734 NT Homo sapiens monoamine oxidase A (MAOA), nuclear gene encoding mitochondrial protein, mRNA 2313 7288 12409 5. 14 8. 0E-63 5031810 NT Homo sapiens IL2-inducible T-cefi kinase (ITK), mRNA 3379 8387 13408 4.89 80.E-63 AF198349. 1 NT Gallus gallus Dach2 protein (Dach2) mRNA, complete cds 3379 8387 13409 4. 89 8. OE-63 AF198349. 1 NT Gallus gallus Dach2 protein (Dach2) mRNA, complete cds 4143 9138 14122 3. 27 8.0E-63 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21 C068 916 5932 1. 84 7.0E-63 AI872137. 1 EST_HUMAN wm55g11. x1 NCI CGAP Ut2 Homo sapiens cDNA clone IMAGE : 2439908 3' 3247 8260 13282 0. 7 4.0E-63 AL163278. 2 NT Homo sapiens chromosome 21 segment HS21 C078 3719 8723 13722 2. 01 4.0E-63 AB014607. 1 NT Homo sapiens mRNA for KIAA0707 protein, partial cds 37198723137232. 014. 0E-63 AB014607. 1'NT Homo sapiens mRNA for KIAA0707 protein, partial cds 1895 6883 11975 2. 673. 0E-63 AB018260. 1 NT Homo sapiens mRNA for KIAA0717 protein, partial cds 2706 7663 12774 1.34 3.0E-63 J00310. 1 NT Human Met-tRNA-i gene 1 2748 6219 11262 10. 16 3.0E-63 6005963 NT Homo sapiens zinc finger protein 144 (Mel-18) (ZNF144), mRNA 191 5255 10267 2. 47 2. 0E-63 U07804. 1 NT Human DNA topol#omerace mRNA, partial cds 198 5262 10275 1. 85 2. OE-63 4885226 NT Homo sapiens eyes absent (Drosophila) homolog 2 (EYA2), mRNA Homo sapiens glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), catalytic (72. 8kD) (GLCLC) 494 5530 1. 36 2. 0E-63 4557624 NT mRNA 816 5837 10872 4. 72 2.0E-63 7657042 NT Homo sapiens Down syndrome candidate region 1 (DSCR1), mRNA 1533 6531 11591 1.52 2.0E-63 AB030388. 1 NT Homo sapiens RHCE mRNA for Rh blood CE group antigen polypeptide, complete cds Page 120 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Too Hit SEQ ID SEQ ID OrF SEQ Expression (Top) Hit Top Hit Acession NO : NO Value 1533 6531 11592 1. 52 2. OE-63 AB030388. 1 NT Homo sapiens RHCE mRNA for Rh blood CE group antigen polypepbde, complete cds 1729 6724 11802 3 2. OE-63 BE410739. 1 EST HUMAN 601301627F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3636103 5' 3083 8099 13114 1.58 2.0E-63 4502166 NT Homo sapiens amytoid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) (APP), mRNA 3212 8227 13249 2.2 2.0E-63 AF109718. 1 NT Homo sapiens chromosome 3 subtelomeric region 3809 8812 13818 1. 4 2. OE-63 L39891. 1 NT Homo sapiens polycystc kidney disease-associated protein (PKD1) gene, complete cds 4716 9701 14687 1. 23 2.0E-63 AF111167. 2 NT Homo sapiens jun dimerization protein gene, partial cds ; cfos gene, complete cds ; and unknown gene 4216 9209 14188 3. 33 1. OE-63 F08485. 1 EST HUMAN HSCZVC111 normalized infant brain cDNA Homo sapiens cDNA clone c-zvd11 4216 9209 14189 3. 33 1. OE-63 F08485. 1 EST HUMAN HScZVD111 mormalized infant brain cDNA Homo sapiens cDNA clone c-zvd11 1029 6039 9. 14 8.0E-64 BE280796. 1 EST HUMAN 601155232F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3139038 5' 3451 8459 0. 9 7.0E-64 BE394321. 1 EST HUMAN 601311455F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3633204 5' 45939581 145703. 257. 0E-64 4507490 NT Homo sapiens thimet oligopeptidase 1 (THOP1) mRNA 4593 9681 14571 3.25 7.0E-64 4507490 NT Homo sapiens thimet oiigopeptidase 1 (THOP1) mRNA wb51eO7. x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2309220 3'similar to gb : M15182 BETA- 1684 6680 11753 5. 45 6.0E-04 Al651992. 1 EST HUMAN GLUCURONIDASE PRECURSOR (HUMAN) ; wb51e07.x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2309220 3'similar to gb : M15182 BETA- 1684 6680 11754 5.45 6.0E-64 AI651992. 1 EST HUMAN GWCURONIDASE PRECURSOR (HUMAN) ; 3049 8066 13074 5. 09 6. OE-64 AW026445. 1 EST HUMAN wv13e03.x1 NCI_CGAP_Brn23 Homo sapiens cDNA clone IMAGE : 2529436 3' 3049 8066 13075 5.09 6.0E-64 AW025445.1 EST_HUMAN wv13e03.x1 NCI_CGAP_Bm23 Homo sapiens cDNA clone IMAGE : 2529436 3' 810 5831 10863 3. 24 5.0E-64 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 810 5831 10864 3.24 5.0E-64 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 1318 6316 11364 2.38 5.0E-64 AB020710. 1 NT Homo sapiens mRNA for KIaA0903 protein, partial cds 1397 6394 11448 1.35 5.0E-64 L40933. 1 NT Homo sapiens phosphoglucomutase-related protein (PGMRP) gene, complete cds 1397 6394 11449 1. 35 5.0E-64 L40933. 1 NT Homo sapiens phosphoglucomutase-related protein (PGMRP) gene, complete cds 1673 6669 11745 1.5 5.0E-64 U89358. 1 NT Human 1 (3) mbt protein homolog mRNA, complete cds 2753 6447 11506 4. 17 5. OE-64 7662205 NT Homo sapiens KIAA0618 gene product (KIAA0618), mRNA 2753 6447 11507 4. 17 5. OE-64 7662205 NT Homo sapiens KIAA0618 gene product (KIAA0618), mRNA 3852 8854 13859 8.23 5.0E-64 AF017433. 1 NT Homo sapiens putative transcription factor CR53 (CR53) mRNA, partial cds 3988 8986 13972 1. 02 5.0E-64 AB020710. 1 NT Homo sapiens mRNA for KIAA0903 protein, partial cds 2135 7115 12228 3.23 3.0E-64 C18895. 1 EST HUMAN C18895 Human placenta cDNA (TFujiwara) Homo sapiens cDNA clone GEN-569E02 5' 3359 8367 13385 1.37 3. 0E-64 AV711714. 1 EST HUMAN AV711714 DCA Homo sapiens cDNA clone DCAAMC01 5' 3359 8367 13386 1. 37 3.0E-64 AV711714.1 EST_HUMAN AV711714 DCA Homo sapiens cDNA clone DCAAMC01 5' 11 6080 11111 1.32 2.0E-64 AA60-9940. 1 EST HUMAN af09d08.s1 Soares_testis_NHT Homo saplens cDNA clone MAGE : 1031151 3' Page 121 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similer Probe Exon Top Hit ORF SEQ Expression (Top) HIt Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor NO : NO : ID NO: Signal BLASTE No. source Value 1374 6371 11420 5. 42 2. 0E-64 4757701 NT Homo sapiens elF4E-like cap-binding protein (4EHP) mRNA wo87b01. x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2462281 3'similar to contains element 2454 7424 1. 92 2. 0E-64 AI927030. 1 EST HUMAN L1 repetitive element ; 2459 7428 12544 2. 92 2. OE-64 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21C046 2459 7428 12545 2. 92 2. 0E-64 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 Homo sapiens glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), 3067 8083 13097 0. 91 2. 0E-64 4504068 NT nuclear gene encoding mitochondrial protein, mRNA 256 5316 10325 1.68 1.0E-64 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA au60c01. x1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2519136 3'similar to 1741 6736 11812 51. 93 1. 0E-64 AI929419. 1 EST HUMAN gb : L21696_eds1 PROTHYMOSIN ALPHA (HUMAN) ; contains elemenbt MSR1 repetitive element ; 2942 7961 12981 0. 81 1. OE-64 4507334 NT Homo sapiens synaptojanin 1 (SYNJ1), mRNA Homo sapiens transcription factor IGHM enhancer 3, JM11 protein, JM4 protein, JM5 protein, T54 protein, JM10 protein, A4 differentiation-dependent protein, triple LIM domain protein 6, and synaptophysin genes, 3432 8440 13467 5 1. OE-64 AF196779. 1 NT complete cds ; and L-type calcium channel a> 3503 8511 13525 1. 46 1. OE-64 AF228527. 1 NT Homo sapiens TRIAD3 mRNA, partial cds 3503 8511 13526 1. 46 1. OE-64 AF228527. 1 NT Homo sapiens TRIAD3 mRNA, partial cds 3805 8808 13814 0. 87 1. 0E-64 8922829 NT Homo sapiens hypothetical protein FLJ11026 (FLJ11026), mRNA 2214 7191 12312 1. 09 9. 0E-65 X89211. 1 NT H. sapiens DNA for endogenous retroviral like eiement 2214 7191 12313 1.09 9.0E-65 X89211.1 NT H.sapiens DNA for endogenous retroviral like element 1039 6049 11079 3. 17 6. 0E-65 AV721898. 1 EST HUMAN AV721898 HTB Homo sapiens cDNA clone HTBBZC06 5' nj86d10. s1 NCI CGAP Pr11 Homo sapiens cDNA clone IMAGE : 999379 similar to gb : K03002 60S 1880 6869 48. 55 6. 0E-65 AA550929. 1 EST HUMAN RIBOSOMAL PROTEIN L32 (HUMAN) ; 625 5652 10656 0. 96 5. 0E-65 AF064604. 1 NT Homo sapiens KE03 protein mRNA, partial cds 1335 6333 11381 1. 94 5. 0E-65 7661951 NT Homo sapiens KIAA0156 gene product (KIAA0156), mRNA 1335 6333 11382 1. 94 5. 0E-65 7661951 NT Homo sapiens KIaA0156 gene product (KIAA0156), mRNA 3181 8197 13220 1. 88 5. 0E-65 4507848 NT Homo sapiens ubiquitin specific protease 13 (isopeptidase T-3) (USP13) mRNA 3181 8197 13221 1. 88 5. OE-65 4507848 NT Homo sapiens ubiquitin specific protease 13 (isopeptidase T-3) (USP13) mRNA 193 5257 10270 3.82 4.0E-85 AL120419. 1 ESTHUMAN DKFZp761G108r1 761 (synonym : hamy2) Homo sapiens cDNA clone DKFZp761G108 5' 736 5759 10781 1. 07 4. 0E-65 A1266468. 1 EST HUMAN qm46e01. x1 Soares_placenta8to9weeks 2NbHP8to9W Homo sapiens cDNA clone IMAGE : 1891800 3' 736 5759 10782 1. 07 4. 0E-65 AI266468. 1 EST_HUMAN qm46e01.x1 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA clone IMAGE : 1891800 3' 1062 607C 11102 6, 88 4. 0E-65 4826735 NT Homo sapiens fragile X mental retardation, autosomal homdog 1 (FXR1), mRNA 1455 6452 11511 18. 94 4. 0E-65 4506636 NT Homo sapiens ribosomal protein L34 (RPL34) mRNA Page 122 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit Probe Exon OrF SEQ Expression Probe ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acesslon Database Top Hit Descriptor ID NO: Signal BLASTE No. NO: NO: Value Source 2275 7251 12368 3. 52 4. 0E-65 BE221469. 1 EST_HUMAN hu25e04.x1 NCI_CGAP_Mel15 Homo sapiens cDNA clone IMAGE : 3171102 3' 2275 7251 12369 3. 52 4. 0E-65 BE221469. 1 EST_HUMAN hu25e04.x1 NCI_CGAP_Mel15 Homo sapiens cDNA clone IMAGE : 3171102 3' 3845 8847 13855 1. 07 4. 0E-65 AW993185. 1 EST_HUMAN RC2-BN0033-160200-013 a03 BN0033 Homo sapiens cDNA 96 5174 10185 1. 82 3. 0E-65 503 1976 NT Homo sapiens pre-B-cell colony-enhancing factor (PBEF) mRNA 97 5174 10185 1. 75 3. 0E-65 5031976 NT Homo sapiens pre-B-cell colony-enhancing factor (PBEF) mRNA 1212 7699 12.12 3.0E-65 X78932.1 NT H.sapiens HZF9 mRNA for zinc finger protein 1527 6524 11581 1. 55 3. 0E-65 4504626 NT Homo sapiens immunoglobin superfamily, member 3 (IGSF3) mRNA, and translated products ov23f03. s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE : 1638173 3'similar to contains element 1787 6779 11871 1. 62 3. 0E-65 A1000692. 1 EST HUMAN MSR1 repetitive element ; 2921 7940 12956 0. 89 3. 0E-65 D87078. 2 NT Homo sapiens mRNA for KIaA0235 protein, partial cds 3203 8218 13241 0. 83 3. OE-65 4504950 NT Homo sapiens laminin, beta 1 (LAMB10, mRNA ov23f03. s1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 1638173 3'similar to contains element 3638 8644 13650 1. 17 3. 0E-65 A1000692. 1 EST HUMAN MSR1 repetitive element ; 4515 9505 14485 1. 45 3. 0E-65 6912385 NT Homo sapiens rab6 GTPase activating protein (GAP and centrosome-associated) (GAPCENA), mRNA 3320 8330 13351 6. 2 2. OE-65 BF680294. 1 EST HUMAN 602155062F1 NIH MGC 83 Homo sapiens cDNA clone IMAGE : 4295966 5' 89 5166 1. 12 1. 0E-65 BF125544. 1 EST_HUMAN 601763488F1 NIH_MGC_20 Homo sapiens cDNA clone IMAGE : 4026501 5' 534 5569 10572 1. 79 1. OE-65 7657495 NT Homo sapiens putative Rab5 GDP/GTP exchange factor homologue (RAB5), mRNA 1989 6974 12079 1. 12 1. 0E-65 AB040946. 1 NT Homo sapiens mRNA for KIAA1513 protein, partial cds 3290 8301 13327 0. 79 1. 0E-65 BE466681. 1 EST_HUMAN hz24a09.x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 3208888 3' 3892 8892 13890 1. 89 1. OE-65 4504082 NT homo saplens glyplcan 4 (GPC4) mRNA 3892 8892 13891 1. 89 1. OE-65 4504082 NT Homo sapiens glypican 4 (GPC4) mRNA 4084 9078 14066 3. 43 1. OE-65 AW029340. 1 EST_HUMAN wx09c09.x1 NCI_CGAP_Gas4 Homo saplens cDNA clone IMAGE:2543152 3' 4084 9078 14067 3.43 1.0E-65 AW029340.l 1 EST_HUMAN wx09c09.x1 NCI_CGAP_Gas4 Homo sapiens cDNA clone IMAGE : 2543152 3' 70 5149 10156 1. 28 9. 0E-66 AL160311. 1 NT Novel human gene mapping to chomosome 22 70 5149 10157 1. 28 9. 0E-66 AL160311. 1 NT Novel human gene mapping to chomosome 22 1336 6334 11383 2. 47 9. 0E-66 5031980 NT Homo sapiens 26S proteasome-associated pad1 homolog (POH1) mRNA 1336 6334 11384 2. 47 9. 0E-66 5031980 NT Homo sapiens 26S proteasome-associated pad1 homolog (POH1) mRNA 1453 6450 3. 8 9. OE-66 M87299. 1 NT Human transposon-like element, partial 4553 9541 14526 0. 96 9. 0E-66 AL137163. 1 NT Novel human gene mapping to chomosome X wn57h07. x1 NCI_CGAP_Lu19 Homo sapiens cDNA clone IMAGE : 2449597 3'similar to WP : F15G9. 4A 4240 9234 142161. 02 6. OE-66 AI924653. 1 EST_HUMAN CE18595 ; wn57h07. x1 NCI CGAP_Lu19 Homo sapiens cDNA clone IMAGE : 2449597 3'similar to WP : F15G9. 4A 4240 9234 14217 1.02 6.0E-66 Al924653.1 EST_HUMAN CE18595 ; Page 123 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most similar Probe Exon Top HIt ORF SEQ Expressaion (Top) HIt Top Hit Acession SEQ ID SEQ ID Databas NO SEQ Expression (lop) Rit Top rut Acession Database Top Hit Decripter NO: NO: Value Source wn57h07. x1 NCI CGAP_Lu19 Homo sapiens cDNA clone IMAGE : 2449597 3'similar to WP : F15G9. 4A 4240 9234 14218 1. 02 6. OE-66 AI924653. 1 EST HUMAN CE18595 ; 1349 6346 11397 1. 86 5. OE-66 BE064410. 1 EST-HUMAN RC4-BT0311-141199-011-h06BT0311 Homo sapiens cDNA 4939 9916 14895 0.81 5.0E-66 BE898644. 1 EST HUMAN 601681592F1 NIH MGC 9 Homo sapiens cDNA clone IMAGE : 3951791 5' 4939 9916 14896 0. 81 5. OE-66 BE898644. 1 EST HUMAN 601681592F1 NIH MGC 9 Homo sapiens cDNA clone IMAGE : 3951791 5' 782 5803 10833 2. 44 4. OE-66 6679816 NT Mus musculus fragile X mental retardation syndrome 1 homolog (Fmr1), mRNA 1698 6693 11769 1.24 4. OE-66 AW897798. 1 EST HUMAN RC1-NN00653-100500-022-a02 NN063 Homo sapiens cDNA 2217 7194 123161 2.12 4. OE-66 X89211. 1 NT H. sapiens DNA for endogenous retroviral like element 2403 7374 2. 81 4. OE-66 AJ223364. 1 NT Homo sapiens germ-line DNA upstream of Jkappa locus 4638 9623 4. 36 4. OE-66 9635487 NT Human endogenous retrovirus, complete genome Homo sapiens solute carrier family 25 (mitochondrial carrier ; adenine nucleotide translocator), member 5 1402 6399 11455 34. 87 3. OE-66 4502098 NT (SLC25A5), nuclear gene encoding mitochondrial protein, mRNA Homo sapiens solute carrier family 25 (mitochondrial carrier ; adenine nucleotide translocator), member 5 1402 6399 11456 34. 87 3. 0E-66 4502098 NT (SLC25A5), nbuclear gene encoding mitochondrial protein, mRNA yz27g12. r1 Soares_multiple_sclerosis_2NbHMSP Homo sapiens cDNA clone IMAGE : 284326 5'similar to 1936 6922 12020 1.02 3.0E-66 N55323.1 EST_HUMAN SW : H2B1 TIGCA P35068 HISTONE H2B. 1/H2B. 2. [2] PIR : B56612 ; yz27g12.r1 Soares_multiple_sclerosls_2NbHMSP Homo sapiens cDNA clone IMAGE : 284326 5'similar to 1936 6922 12021 1.02 3. OE-66 N55323. 1 EST HUMAN SW : H2B1 TIGGA P35068 HISTONE H2B. 1/H@B, 2. [2] PIR : B56612 ; yz27g12. r1 Soaresmu) tipfescferosis2NbHMSP Homo sapiens cDNA clone IMAGE : 284326 5'similar to 1936 6922 12022 1. 02 3. 0E-66 N55323. 1 EST HUMAN SW : H2B1 TIGCA P35068 HISTONE H2B. 1/H2B. 2. [2] PIR : B56612 ; 2635 7595 12708 2. 42 3. OE-66 11141880 NT Homo sapiens TGF (beta)-induced transcription factor 2 (TGIF2), mRNA 3043 8060 13069 7. 45 3.0E-66 7662223 NT Homo sapiens KLAA0649 gene product (KIAA0649), mRNA 52 5133 10128 1.38 2. OE-66 7657334 NT Homo sapiens Misshapen/NiK-related kinase (MINK), mRNA 52 5133 10129 1. 38 2. OE-66 7657334 NT Homo sapiens Misshapen/NIK-related kinase (MINK), mRNA Homo sapiens origin recognition complex, subunit 5 (yeast homolog)-like (ORC5L) mRNA, and translated 419 5077 10061 1. 14 2. OE-66 4505524 NT products Homo sapiens origin recognition complex, subunit 5 (yeast homolog)-like (ORC5L) mRNA, and translated 419 5077 10062 1. 14 2. 0E-66 4505524 NT products 1791 6782 11874 2. 52 2. OE-66 AL163301. 2 NT Homo sapiens chromosome 21 segment HS21 C101 2905 7924 12943 0.98 2. OE-66 X65859. 1 NT H. sapiens pseudogene for the low affinity IL-8 receptor 3679 8684 13686 0. 82 2. 0E-66 AL117233. 1 NT Novel human gene mapping to chomosome 1 4137 9132 14115 0.91 2. 0E-66 8923768 NT Homjo sapiens histone deacetylase 8 (HDAC8 gene) (HSA277724), mRNA 452 9511 14495 39. 41 2. 0E-66 AJ133267. 2 NT Homo sapiens HLA-B gene for human leucocyte antigen B 4521 9511 14496 39. 41 2. 0E-66 AJ133267. 2 NT Homo sapiens HLA-B gene for human leucocyte antigen B Page 124 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor NO: NO: ID NO: Signal BLAST E No. Sourie Vaque 1644 6640 1. 17 1. OE-66 BE887173. 1 EST HUMAN 601508376F1 NIH_MGC_71 Homo sapiens cDNA clone IMAGE : 3909931 5' 2823 7844 128631. 49 1. OE-66 AV717817. 1 EST HUMAN AV717817 DBC Homo sapiens cDNA clone DCBADC07 5' 2823 7844 12864 1.49 1.0E-66 AV717817.1 EST_HUMAN AV717817 DBC Homo sapiens cDNA clone DCBADC07 5' 4260 7844 12863 3.88 1. 0E-66 AV717817. 1 EST HUMAN AV717817 DCB Homo sapiens cDNA clone DCBADC07 5' 4260 7844 12864 3. 88 1. OE-66 AV717817. 1 EST HUMAN AV717817 DCB Homo sapiens cDNA clone DCBADC07 5' au75d02. x1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2782083 3'similar to gb : M37104 379 5454 10471 4. 95 7. 0E-67 AW 162232. 1 EST_HUMAN ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL PRECURSOR (HUMAN) ; EST96812 Testis Homo sapiens cDNA 5'end similar to similar to C. elegans hypothetical protein, cosmid 1363 6360 11410 2. 15 7. 0E-67 AA383461. 1 ESTHUMAN ZK353 1523 6520 11576 1. 98 7. 0E-67 W85947. 1 EST HUMAN zh56b05.r1 Soares_fetal_liver_spleen_1NFLS-S1 Homo sapiens cDNA clone IMAGE : 416049 5' 1523 6520 11577 1. 98 7. 0E-67 W85947. 1 EST HUMAN zh56b05.r1 Soares_fetal_liver-spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 416049 5' 1982 6967 12072 1. 31 7. OE-67 7657243 NT Homo sapiens inositol 1, 3, 4-triphosphate 5/6 kinase (ITPK1), mRNA 1982 696 12073 1. 31 7. OE-67 7657243 NT Homo sapiens inositol 1, 3, 4-triphosphate 5/6 kinase (ITPK1), mRNA au75d02. x1 Schneider fetal brain 00004 Homo sapiens CDNA clone IMAGE : 2782083 3'similar to gb : M37104 2738 5454 10471 4. 77 7. 0E-67|AW162232. 1 EST HUMAN ATP SYNTHASE COUPLING FACTOR 6, MITOCHONDRIAL PRECURSOR (HUMAN) ; 555 5589 10590 1. 53 6. OE-67 X68968. 1 NT H. sapiens mRNA for acetyl-CoA carboxylase 787 5808 10838 2.36 6.0E-67 Z17227. 1 NT Homo sapiens mRNA for transmebrane receptor protein 1254 6252 11293 1. 35 6. OE-67 Y14320. 1 NT Homo sapiens PMP69 gene, exons 3, 4, 5, 6 & 7 3095 8111 13129 1.52 6.0E-67 4506434 NT Homo sapiens retinoblastoma 1 (including osteosarcoma) (RB1) mRNA 3353 8361 13377 1. 44 6.0E-67 4507332 NT Homo sapiens Synapsin III (SYN3) mRNA, and translated products 3353 8361 13378 1.44 6. 0E-67 4507332 NT Homo sapiens Synapsin III (SYN3) mRNA, and translated products 4567 9555 14542 3. 45 6.0E-67 7657020 NT Homo spaiens DKFZp434P211 protein (DKFZP434P211), mRNA 4567 9555 14543 3.45 6. 0E-67 7657020 NT Homo sapiens DKFZp434P211 protein (DKFZP434P211), mRNA 4805 9789 0. 86 6. OE-67 4507848 NT Homo sapiens ubiquitin specific protease 13 (isopeptidase T-3) (USP13) mRNA 5012 9983 14958 1. 01 6.0E-67 AF016898. 1 NT Homo sapiens B-ATF gene complete cds 5012 9983 14959 1. 01 6. OE-67 AF016898. 1 NT Homo sapiens B-ATF gene, complete cds 3150 8166 13186 2. 02 5. 0E-67 AF009660. 1 NT Homo sapiens T cell receptor beta locus, TCRBV7S3A2 to TCRBV12S2 region 1308 6306 11354 1. 2 4. 0E-67R90819. 1 EST HUMAN ny02d11.r1 Soares adult brain N2b4hB55Y Homo sapiens cDNA clone IMAGE : 167253 5' 2741 5654 10659 1.65 3.0E-67 AA333768. 1 EST-HUMAN EST37903 Embryo, 9 week Homo sapiens cDNA 5'end 3371 8379 13399 1.01 3. 0E-67 BE064410. 1 EST HUMAN RC4-BT0311-141199-011-h06 BT0311 Homo sapiens cDNA 4555 9543 14528 3. 04 3. OE-67 AW869159. 1 ESTHUMAN MR3-SN0066-040500-008-f01 SN0066 Homo sapiens cDNA hw16g09. x1 NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3183136 3'similar to WP : F23H11. 9 188 5252 10263 1.1 2.0E-67 BE348354. 1 EST HUMAN CE09617 ; 835 5854 10894 5. 38 2. OE-67 AW816405. 1 EST HUMAN QV4-ST0234-181199-037-f05 ST0234 Homo sapiens cDNA Page 125 of 209 Table 4 Singie Exon Proues Expressed in HRI 100 Cells Probe Exon Most Similar SEQ ORF SEQ Expressin (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. source NO: NO: Source Value 1088 6095 2.11 2.0E-67 AF167460.1 NT Homo sapiens double stranded RNA activaled protein kinase (PKR) gene, exons 2a, 2, 3, and ba72g0.5y1 NIH_MGC-20 Homo sapiens cDNA clone IMAGE:2905976 5' similar to TR:O94892 O94892 1845 6834 11924 1. 28 2. OE-67 BE303037. 1 EST HUMAN KIAA0798 PROTEIN. ; ba72g05. y1 N) HMGC20 Homo sapiens cDNA clone IMAGE : 2905976 5'similar to TR : 094892 094892 1845 6834 11925 1. 28 2. 0E-67 BE303037. 1 EST-HUMAN KIAA0798 PROTEIN. ; 2179 7158 12277 1. 81 2. OE-67 11422946 NT Homo sapiens hypothetical protein dJ462023. 2 (DJ462023. 2), mRNA 2179 7158 12278 1. 81 2. OE-67 11422946 NT Homo sapiens hypothetical protein dJ462023. 2 (DJ462023. 2), mRNA 2323 7297 12419 1. 29 2. 0E-67 AF309561. 1 NT Homo sapiens KRAB zinc finger protein ZFQR mRNA, complete cds I 2364 7338 12455 2. 28 2. 0E-67 4758795 NT Homo sapiens developmentally regulated GTP-binding protein 1 (DRG1), mRNA 3387 8395 13419 3. 66 2. 0E-67 AA625755. 1 EST_HUMAN zu91g01.s1 Soares)_testis_NHT Homo sapiens cDNA clone IMAGE : 745392 3' 3896 8896 13894 2. 87 2. 0E-67 AL163300. 2 NT Homo sapiens chromosome 21 segment HS21 C100 251 5311 10322 2. 94 1. OE-67 4502166 NT omo saplens amyioid beta (A4) precursor protein (protease nexin-ii, Alzheimer disease) (APP), mRNA 698 5722 10738 2. 16 1. OE-67 AA702794. 1 EST_HUMAN zi90b04.s1 SoaresA_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 448015 3' 2111 7091 12205 2. 23 8. 0E-68 BE870732. 1 EST_HUMAN 601448558F1 NIH_MGC_65 homo sapiens cDNA clone IMAGE: 3852254 5' zq82h10. r1 Stratagene hNT neuron (#937233) Homo sapiens cDNA clone IMAGE : 648163 5'similar to i 3778 8781 13784 4.83 8. 0E-68 AA209456. 1 EST HUMAN SW : SAV SULAC Q07590 SAV PROTEIN. ; zq82h10. r1 Stratagene hNT neuron (#937233) Homo sapiens cDNA clone IMAGE : 648163 5'similar to 3778 8781 13785 4. 83 8. 0E-68 AA209456. 1 EST HUMAN SW : SAV_SULAC Q07590 SAV PROTIEN. ; 1851 6840 1. 93 6. 0E-68 AW503842. 1 EST_HUMAN UI-HF-BN0-alb-c-07-0-Ul.r1 NIH_MGC_50 Homo sapiens cDNA clone IMAGE : 3078924 5' 809 5830 10861 3. 82 5. 0E-68 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 809 5830 10862 3. 82 5. 0E-68 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 2707 7664 12775 1. 09 5. 0E-68 AF231919. 1-NT Homo sapines chromosome 21 unknown mRNA 3074 8090 13104 3. 02 5. 0E-68 AB037852. 1 NT Homo sapiens mRNA for KIAA1431 protein, partial cds 4057 9051 0. 68 5. OE-68 4826967 NT Homo sapiens retinoblastoma-binding protein 2 (RBBP2) mRNA 4363 9354 14333 0. 66 5. 0E-68 AL157645. 1 EST_HUMAN DKFZp547D207_R1 547 (synonym: hfbr1) Homo sapiens cDNA clone DKFZp547D207 5' 2456 7426 12540 1. 15 4. OE-68 11421388 NT Homo sapiens transcription factor NRF (NRF), mRNA 2456 7426 12541 1. 15 4. 0E-68 11421388 NT Homo sapiens transcription factor NRF (NRF), mRNA 4816 9800 16. 14 4.0E-68 P04406 SW ISSPROT GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, LIVER 3577 8584 13589 7. 2 3.0E-68 AF236082. 1 NT Mus musculus G-protein coupled receptor GPR73 (Gpr73) mRNA, complete cds 2791 10054 39. 36 2. 0E-68 D00522. 1 NT Cricetulus longicaudatus mRNA for EF-1 alpha, complete cds 7f15f02. x1 NCI-CGAP-CLL1 Homo sapiens cDNA clone IMAGE : 3294747 3'similar to TR : 080828 080828 3915 8915 13909 0. 68 2. 0E-68 BE675766. 1 EST HUMAN HYPOTHETICAL 88. 8 KD PROTEIN. ; 4543 9532 14520 1. 64 2. 0E-68 AB008681. 1 NT Homo sapiens gene for activin receptor type IIB, complete cds Page 126 of 209 Table 4 Sinale Exon Probes Exnressed in HBL100 Cells-' Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databas@ ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: NO: Source Value 78 5155 10168 0.94 1.0E-68 4505222 NT Homo sapiens meningioma (disrupted in baianced transtocation) 1 (MN1), mRNA 294 5351 10364 11. 12 1. OE-68 AW816405. 1 EST HUMAN QV4-ST0234-181199-037-f05 ST0234 Homo sapiens cDNA 2190 7169 12289 1. 74 1. OE-68 AB011149. 1 NT Homo sapiens mRNA for KIAA0577 protein, complete cds 2190 7169 12290 1.74 1.0E-68 AB011149. 1 NT Homo sapiens mRNA for KIAA0577 protein, complete cds 3903 8903 13901 0.97 1.0E-68 BE296032. 1 EST HUMAN 601177002F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 3532344 5' 4901 9880 14849 1.18 1.0E-68 BE296032. 1 EST HUMAN 601177002F1 NIH_MGC 17 Homo sapiens cDNA clone IMAGE : 3532344 5 20 5100 10083 7. 87 9. OE-69 5031976 NT Homo sapiens pre-B-cell coiony-enhancing factor (PBEF) mRNA 20 5100 10084 7. 87 9. OE-69 5031976 NT Homo sapiens pre-B-cell cdony-enhancing factor (PBEF) mRNA 1011 6021 11050 2. 59 9. OE-69 5031980 NT Homo sapiens 26S proteasome-associated pad1 homolog (POH1) mRNA 1011 6021 11051 2.59 9.0E-69 5031980 NT Homo sapiens 26S proteasome-associated pad1 homolog (POH1) mRNA 5035 10006 14977 0. 94 9. OE-69 4507164 NT Homo sapiens nuclear antigen Sp100 (SP100) mRNA 3302 8313 1. 28 8. OE-69 AJ237744. 1 NT Homo sapiens RIBIIR gene (partial), exon 12 516 5551 0.99 4.0-E-69 Al873630. 1 EST HUMAN wm26h11. x1 NCI CGAP Ut4 Homo sapiens cDNA clone IMAGE : 2437125 3' 385 5460 10478 5.07 3.0E-69 BE258012. 1 EST_HUMAN 601110371F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE : 3351352 5' 6085635 10636 2. 03 3.0E-69 AF221712. 1 NT Homo sapiens Smad-and Olf-interacting zinc finger protein mRNA, partial cds yd08a02. r1 Scares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 24880 5'similar to SP : A48836 1524 6521 1.63 3.0E-69 T80514. 1 EST_HUMAN A48836 SPEGF III=EGF REPEAT-CONTAINING FIBROPELLIN-LIKE PROTEIN-SEA URCHIN ; 4804 9788 14771 0. 97 3. OE-69 AB037732. 1 NT Homo sapiens mRNA for KJAA1311 protein, partial cds 4971 8965 13954 1.66 3.0E-69 Al765888. 1 EST_HUMAN wh66g08.x1 NCl_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 2385758 3' 129 5438 10457 2. 01 2. OE-69 AF160252. 1 NT Homo sapiens KIAA0553 protein gene, complete cds ; and alphallb protein gene, partial cds 129 5438 10458 2.01 2.0E-69 AF160252. 1 NT Homo sapiens KIAA0553 protein gene, complete cds ; and aiphallb protein gene, partial cds 402 5438 10457 5. 22 2.0E-69 AF160252. 1 NT Homo sapiens KIAA0553 protein gene, complete cds ; and alphatlb protein gene, partial cds 4025438 10458 5. 22 2.0E-69 AF160252. 1 NT Homo sapiens KIAA0553 protein gene, complete cds ; and alphallb protein gene, partial cds 1846 6835 11926 2. 59 2. OE-69 BE257857. 1 EST_HUMAN 601109444F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE : 3350074 5' 2770 7791 3. 44 2.0E-69 AA431157. 1 EST HUMAN zw71g02. r1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 781682 5' 4970 9947 14924 1. 2 2. OE-69 4504148 NT Homo sapiens glutamate receptor, metabotropic 8 (GRM8) mRNA 4970 9947 14925 1. 2 2. 0E-69 4504148 NT Homo sapiens glutamate receptor, metabotropic 8 (GRM8) mRNA 1664 6660 11734 1.7 1.0E-69 AF053768. 1 NT Rattus norvegicus brain specific cortactin-binding protein CBP90 mRNA, partial cds 2270 7766 12363 1. 55 8. OE-70 AA230303. 1 EST_HUAN nc13d12.r1 NCl_CGAP_Pr1 Homo sapiens cDNA clone IMAGE : 1008023 ! 4248 9242 14226 2 8.0E-70 L77566.1 NT Homo sapiens DGS-I mRNA, 3'end 1777 6769 11860 4. 59 7.0E-70 AI497807.1 EST-HUMAN tm89f01.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE : 2165305 3' 1777 6769 11861 4.59 7.0E-70 AI497807.1 EST_HUMAN tm89f01.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE : 2165305 3' 1890 6879 11969 2.37 7.0E-70 AA282955. 1 EST HUMAN zt15h04. r1 NCI CGAP GCB1 Homo sapiens cDNA clone IMAGE : 713239 5' Page 127 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Similar SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acessoin Database Top Hit Descriptor NO: 1 NO : Signal BLAST E No. Source Value 2011 6994 6.81 7.0E-70 5031668 NT Homo sapiens tumor suppressor deleted in oral cancer-related 1 9DOC-1R) mRNA 4103 9097 14083 3. 55 7. OE-70 4757723 NT Homo sapiens adenylate cyclase 3 (ADCY3) mRNA 860 5879 10920 4. 09 6. 0E-70 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nexin-11, Alzheimer disease) (APP), mRNA 2078 7059 12169 4. 32 6. 0E-70 M30938. 1 NT Human Ku (p70/p80) subunit mRNA, complete cds 2437 7408 12525 1. 12 6. OE-70 8923899 NT Homo sapiens CMP-N-acetyineuraminic acid synthase (LOC55907), mRNA 2481 7772 12561 1.53 5.0E-70 7662307 NT Homo sapiens KIAA0792 gene product (KIAA0792), mRNA 2481 7772 12562 1. 53 5. OE-70 7662307 NT Homo sapiens KIAA0792 gene product (KIAA0792), mRNA 1554 6551 11610 3. 24 3. 0E-70 BE071796. 1 EST HUMAN RCO-BT0522-071299-011-a12 BT0522 Homo sapiens cDNA 1554 6551 11611 3. 24 3. 0E-70 BE071796. 1 EST_HUMAN RC0-BT0522-071299-011-a12 BT0522 Homo sapiens cDNA 39 5119 10107 1.16 2. 0E-70 AF012872. 1 NT Homo sapiens phosphatidylinositol 4-kinase 230 (pi4K230) mRNA, complete cds yy07a10. r1 Soares mefanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 270522 5'similar to 680 5705 10714 11. 75 2. 0E-70 N42161. 1 EST HUMAN SW : D3HI_RAT P29266 3-HYDROXYISOBUTYRATE DEHYDROGENASE PRECURSOR ; yy07a10. r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 270522 5'similar to 680 5705 10715 11. 75 2. 0E-70 N42161. 1 EST_HUMAN SW : D3HI_RAT P29266 3-HDYROXYISOBUTYRATE DEHYDROGENASE PRECURSOR ; 695 5719 10737 1.73 2.0E-70 AI246899. 1 EST HUMAN qx51h01. x1 NCI CGAP Pan1 Homo sapiens cDNA clone IMAGE : 2004913 3' 1005 6015 11045 2.33 2. 0E-70 8923669 NT Homo sapiens hypothetical protein FLJ20758 (FLJ20758), mRNA 1165 6168 11202 1. 5 2. 0E-70 7661983 NT Homo sapiens KIAA0193 gene product (KIAA0193), mRNA 1165 6168 11203 1. 5 2. OE-70 7661983 NT Homo sapiens KIAA0193 gene product (KIAA0193), mRNA zp45h05. r1 Stratagene HeLa cell s3 937216 Homo sapiens cDNA clone IMAGE : 612441 5'similar to 1615 6612 11678 1. 22 2. 0E-70 AA180093. 1 EST HUMAN TR : G1041293 G1041293 D2085. 5 ; zp45h05. r1 Stratagene HeLa cell s3 937216 Homo sapiens cDNA clone IMAGE : 612441 5'similar to 1615 6612 11679 1.22 2. 0E-70 AA180093. 1 EST HUMAN TR : G1041293 G1041293 D2085. 5 ; 1703 6698 11774 1. 85 2. 0E-70 AL163202. 2 NT Homo sapiens chromosome 21 segment HS21C002 zf48g04. r1 Soares retina N2b4HR Homo sapiens cDNA clone IMAGE : 380214 5'similar to SW : GAGHTL1A 2256 7233 7. 98 2. 0E-70 AA054010. 1 EST-HUMAN P03345 GAG POLYPROTEIN ; 2415 7386 12506 1. 57 2. 0E-70 AB011173. 1 NT Homo sapiens mRNA for KIAA0601 protein, partial cds 3728 8732 13730 1. 26 2. OE-70 AL133207. 2 NT Novel human gene mapping to chomosome X 3838 8840 13847 1.17 2.0E-70 AJ246003. 1 NT Homo sapiens Spast gene for spastin protein 3936 8935 13928 4. 94 2. 0E-70 M69181. 1 NT Human nonmuscle myosin heavy chain-B (MYH10) mRNA, partial cds 4069 9063 14051 1. 03 2. 0E-70 L78810. 1 NT Homo sapiens ADP/ATP carrier protein (ANT-2) gene, complete cds 4069 9063 14052 1. 03 2. 0E-70 L78810. 1 NT Homo sapiens ADP/ATP carrier protein (ANT-2) gene, complete cds Homo sapiens transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyltransferase) (TGM3) 3309 8320 3. 18 1. OE-70 4507476 NT mRNA Page 128 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession pabase Top Hit Descriptor ID NO : Signal BLAST E N NO : NO : Value. 2148 7127 12244 14. 02 5. OE-71 AF056322. 1 NT Homo sapiens SP100-HMG nuclear autoan6gen (SP100) mRNA, complete cds 4001 8997 13986 0. 87 5. OE-71 AW 816405. 1 EST HUMAN QVT0234-181199-037-f05 ST0234 Homo sapiens cDNA 103 5180 10191 0. 97 4. OE-71 4507592 NT Homo sapiens tumor necrosis facta (ligand) superfamily, member 10 (TNFSF10) mRNA 348 5400 10409 215. 31 4. OE-71 AF157626. 1 NT Equus caballus glyceraldehyde-3-phosphate dehydrogenase mRNA, part'al cds 348 5400 10410 215. 31 4. OE-71 AF157626. 1 NT Equus caballus giyceraldehyde-3-phosphate dehydrogenase mRNA, partial cds 2816 7836 12851 1. 81 4. OE-71 4505880 NT Homo sapiens plasminogen (PLG) mRNA 4304 9296 14282 7. 98 4. OE-71 AF056322. 1 NT Homo sapiens SP900-HMG nuclear autoantigen (SP100) mRNA, complete cds 4827 9811 14792 6. 12 4. OE-71 7657602 NT Homo sapiens putative heme-binding protein (SOUL), mRNA 1210 6210 11250 16. 61 2. OE-71 AL163206. 2 NT Homo sapiens chromosome 21 segment HS21 C006 oy15e03. s1 Soares senescent fibroblasts NbHSF Homo sapiens cDNA clone IMAGE : 1665916 3'similar to 632 5660 10665 1. 87 1. OE-71 A1077927. 1 EST HUMAN contains LOR1. b2 LOR1 repe66ve element ; g27 5g44 10978 6. 13 1. OE-71 7706281 NT Homo sapiens neuronal cell death-related protein (LOC51616), mRNA 1083 6090 11119 4. 33 1. OE-71 AF205890. 1 NT Homo sapiens disabled-2 gene, exons 2 through 15 and compiete cds 1320 6318 11366 8. 38 1. OE-71 AF012872. 1 NT Homo sapiens phospha6dylinositol 4-kinase 230 (pi4K230) mRNA, complete cds 2029 7012 12120 1. 35 1. OE-71 AB017007. 1 NT Homo sapiens PMS2L16 mRNPc, partial cds 2p29 7012 12121 1. 35 1. OE-71 AB017007. 1 NT Homo sapiens PMS2L16 mRNA, partial cds 2621 7583 12693 3. 09 1. OE-71 7657153 NT Homo sapiens hairylenhancer-of-split relaterJ with YRPW mo6f-like (HEYL), mRNA 3422 8430 13456 2. 36 1. OE-71 AF119665. 1 NT Homo sapiens inorganic pyrophosphatase mRNA, complete cds 3516 8524 13534 5. 34 1. OE-71 AF246219. 1 NT Homo sapiens SNARE protein kinase SNAK mRNA, complete cds 3516 8524 13535 5. 34 1. OE-71 AF246219. 1 NT Homo sapiens SNARE protein kinase SNAK mRNA, complete cds 02 15 Human Epidermal Kera6nocyte Subtraction Library-Upregulated 3563 8570 13575 0. 73 1. OE-71 BE122850. 1 EST HUMAN clone 02 15 5'similar to Homo sapiens chromosome 19 02_15 Human Epidermal Kera6nocyte Subtraction Library-Upregulated Transcripts Homo sapiens cDNA 3563 8570 13576 0. 73 1. OE-71 BE122850. 1 EST HUMAN clone 02_15 5'similar to Homo sapiens chomosome 19- 3656 8662 13667 1. 73 1. OE-71 AF218904. 1 NT Homo sapiens attrac6n precursor (ATRN) gene, exon 19 4345 9336 14319 2. 05 1. OE-71 D28476. 1 NT Human mRNA for KIAA0045 gene, complete cds 4460 9450 14431 1. 19 1. OE-71 H23176. 1 EST HUMAN ym56h10. r1 Soares infant brain 1 NIB Homo sapiens cDNA clone IMAGE : 52528 5' wk95g03. x1 NCI CGAP Lu19 Homo sapiens cDNA clone IMAGE : 2423188 3'similar to TR : 086705 086705 405 5441 10460 0. 74 9. OE-72 AI857635. 1 EST HUMAN HYPOTHETICAL 38. 6 KD PROTEIN. ; contains Alu repeti6ve element ; wk95g03. x1 NCI CGAP Lu19 Homo sapiens cDNA clone IMAGE : 2423188 3'simila to TFt : 086705 086705. 405 5441 10461 0. 74 9. OE-72 AI857635. 1 EST HUMAN HYPOTHETICAL 38. 6 KD PROTEIN. ; contains Alu repebGve element ; 3996 8992 13977 1. 54 7. OE-72 4501866 NT Homo sapiens aconitase 2, mitochondrial (AC02), nuclear gene encoding mitocondrial protein, mRNA i : Page 129 of 209 Table 4 Single Fxnn Pmbes Expressed in HRI 100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value 3996 8992 13978 1. 54 7. 0E-72 4501866 NT Homo sapiens aconitese 2, mitochondrial (AC02), nuclear gene encoding mitocondrial protein, mRNA 3996 8992 13979 1. 54 7. 0E-72 4501866 NT Homo sapiens aconitase 2, mitochondrial (AC02), nuclear gene encoding mitocondrial protein, mRNA 64 5144 10148 2. 81 5. 0E-72 BF333707. 1 EST HUMAN QVO-CS0010-150900-398-e11 CS0010 Homo sapiens cDNA 64 5144 10149 2. 81 5. 0E-72 BF333707. 1 EST HUMAN QVO-CS0010-150900-398-e11 CS0010 Homo sapiens cDNA 65 5144 10148 18. 08 5. 0E-72 BF333707. 1 EST HUMAN QVO-CS0010-150900-398-e11 CS0010 Homo sapiens cDNA 65 5144 10149 18.08 5. 0E-72 BF333707. 1 EST_HUMAN QV0-CS0010-150900-398-e11 CS0010 Homo sapiens cDNA 1122 6128 2. 73 5. 0E-72 L11645. 1 NT Homo sapiens alpha-tubulin MRNA, complete cds 4676 9661 1. 32 4. 0E-72 11034844 NT Homo sapiens hypothetical protein dJ1057B20. 2 (DJ1057B20. 2), mRNA 5048 10019 14988 1. 07 4. 0E-72 AB033104. 1 NT Homo sapiens mRNA for KIAA1278 protein, partial cds 19 5099 10082 2. 48 3. 0E-72 5031976 NT Homo sapiens pre-B-cell colony-enhancing factor (PBEF) mRNA 892 5910 1. 27 3. 0E-72 AA723823. 1 EST_HUMAN ah63a06.s1 Soares_testis_NHT Homo sapiens cDNA clone 1310290 3' 1137 6142 11171 11. 37 3. 0E-72 U16306. 1 NT Human chondroitin sulfate proteoglycan versican VO splice-variant precursor peptide mRNA, complete cds 1137 6142 11172 11. 37 3. 0E-72 U16306. 1 NT Human chondroitin sulfate proteoglycan versican VO splice-variant precursor peptide mRNA, complete cds 1174 6177 11211 1. 12 3. 0E-72 U80226. 1 NT Human gamma-aminobutyric acid transaminase mRNA, partial cds 1174 6177 11212 1.12 3.0E-72 U80226.1 NT Human gamma-aminobutyric acid transaminAse mRNA, partial cds 3001 8019 13032 12. 79 3. 0E-72 AJ229043. 1 NT Homo sapiens 959 kb contig between AML1 and CBR1 on chromosome 21q22, segment 3/3 3205 8220 13242 2. 5 3. OE-72 8923548 NT Homo sapiens hypothetical protein FLJ20585 (FLJ20585), mRNA TCR V delta 2-C alpha =T-cell receptor delta and C alpha fusion gene {alternatively spliced, splice junction) 3732 8736 13734 3. 01 3. 0E-72 S77589. 1 NT (humain, precursor B-cell line REH, mRNA Partial, 211 nt] 4414 9404 14389 3. 55 3. 0E-72 11416196 NT Homo sapiens hypothetical protein (FLJ11127), mRNA 4621 9606 14593 1. 08 3. OE-72 AF167572. 1 NT Homo sapiens protein methyttransferase (JBP1) mRNA, complete cds 4621 9606 14594 1. 08 3. 0E-72 AF167572. 1 NT Homo sapiens protein mehtyltransferase (JBP1) mRNA, complete cds 2022 7005 12110 1.03 1.0E-72 AA846225.1 EST_HUMAN ai83d02.s1 Soares_parathyroid_tumor-NbHPA Homo sapiens cDNA clone IMAGE : 1387395 3' 1433 6430 11486 1. 25 9. 0E-73 AW374968. 1 EST HUMAN MRO-CT0063-071099-002-h11 CT0063 Homo sapiens cDNA ws55c06. x1 NCI_CGAP Brn25 Homo sapiens cDNA clone IMAGE : 2501098 3'similar to TR : Q59050 1021 6030 11060 3. 27 8. 0E-73 AW071755. 1 EST HUMAN Q59050 HYPOTHETICAL PROTEIN MJ1656. ; 1117 6123 11153 0. 98 7. 0E-73 8923290 NT Homo sapiens hypothetical protein FLJ20309 (FLJ20309), mRNA 3227 8242 13264 1. 64 7. 0E-73 AL163206. 2 NT Homo sapiens chromosome 21 segment HS21 C006 4787 9771 1.48 7. 0E-73 AL163282. 2 NT Homo sapiens chromosome 21 segment HS21C082 157 5223 1. 71 6. 0E-73 AL163218. 2 NT Homo sapiens chromosome 21 segment HS21C018 Page 130 of 209 Table 4 SinnlP Fxtzn Prnhae FYnracal in HRI 9f1f1 fclle Probe Exon Most similar SEQ ID SEQ ID ORF SEQ Expressoin (Top) Hit Top Hit Acession Top Hit NO NO: Signal BLAST E No. Database toph It Descriptor Value 1321 6319 11367 1. 38 3. OE-73 AW843789. 1 EST HUMAN CMO-CN0044-260100-164-f08 CN0044 Homo sapiens cDNA 1825 6815 11909 1. 1 3. 0E-73 11435913 NT Homo sapiens heme-binding protein (HEBP), mRNA 1825 6815 11910 1.1 3. OE-73 11435913 NT Homo sapiens heme-binding protein (HEBP), mRNA 841 5860 10900 2. 37 2. OE-73 AF139897. 1 NT Homo sapiens BASS1 (BASS1) mRNA, partial cds 1906 6893 3. 12 2. OE-73 AW898081. 1 EST HUMAN RC3-NN0066-270400-011-c04 NN0066 Homo sapiens cDNA 3108 8124 13143 3. 99 2. OE-73 4502582 NT Homo sapiens caspase 8, apoptosis-related cysteine protease (CASP8) mRNA 4311 9303 1. 02 2. OE-73 AL163283. 2 NT Homo sapiens chromosome 21 segment HS21C083 1745 6740 11819 2. 61 1. OE-73 AU121585. 1 EST_HUMAN AUX121585 MAMMA1 Homo sapiens cDNA clone MAMMA1000490 5' 2413 7384 12503 1. 04 1. OE-73 AF198349. 1 NT Gallus gallus Dach2 protein (Dach2) mRNA, complete cds 731 5754 10775 1. 76 8. 0E-74 4557426 NT Homo sapiens CD39-like 4 (CD39L4) mRNA 1909 6895 11989 2. 57 7. 0E-74 AJ001689. 1 NT Homo sapiens NKG2D gene, exon 10 3253 8266 13288 1. 22 7. 0E-74 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 Homo sapiens S164 gene, partial cds ; PS1 and hypothetical protein genes, complete cds ; and S171 gene, 1105 6112 11143 4 6. 0E-74 AF109907. 1 NT partial cds 1587 6584 11645 0. 92 6. 0E-74 AW263177. 1 ESTHUMAN xn78g07. x1SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2700636 3' 2252 7229 12347 53. 86 6. 0E-74 BE388260. 1 EST=UMAN 601283521F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3605453 5' 2252 7229 12348 53. 86 6. OE-74 BE388260. 1 EST HUMAN 601283521 F1 NIH MGC 44 Homo sapiens cDNA clone IMAGE : 3605453 5' 2793 7813 12831 0. 97 6. 0E-74 AW014039. 1 EST HUMAN Ul-H-BIO-aah-h-03-0-Ul. s1 NCI CGAP Sub1 Homo sapiens cDNA clone IMAGE : 2709365 3' 2793 7813 12832 0. 97 6. 0E-74 AW014039. 1 EST-HUMAN UI-H-BI0-aah-h-03-0-Ul.s1 NCI_CGAP_Sub1 Homo sapiens cDNA clone IMAGE : 2709365 3' 3631 8637 13642 1. 37 6. 0E-74 BE048846. 1 EST_HUMAN hr54e11.x1 NCI-CGAP_Kld11 Homo sapiens cDNA clone IMAGE:3132332 3' 3631 8637 13643 1. 37 6. 0E-74 BE048846. 1 EST_HUMAN hr54e11.x1 NCI_CGAP_Kid11 Homo sapiens cDNA clone IMAGE : 3132332 3' Homo spaiens DEAD/H 9Asp-Glu-Ala-Asp/His) box polypeptide 11 (S.cerevisiae CHL1-like halicase) 4866 9846 14821 1.55 6.0E-74 4758135 NT (DDX11) mRNA Homs sapiens DEAD/H 9Asp-Glu-Ala-Asp/His0 box polypeptide 11 (S.cereviciee CHL1-like helicase) Homo sapiens DEAD/H (Asp-Glu-Aia-Asp/His) box polypeptide 11 (S. cerevisiae CHL, 1-like helicase) 4866 9846 14822 1. 55 6. 0E-74 4758135 NT (DDX11) mRNA 894 5912 10951 2. 15 5. OE-74 AW020986. 1 EST NUMAN df17c09. y1 Morton Fetal Cochlea Homo sapiens cDNA clone IMAGE : 2483704 5' 2630 7590 6. 51 5. OE-74 AW362756. 1 ESTHUMAN PMO-CT0289-271099-001-h07CT0289 Homo sapiens cDNA 277 5335 10349 5.48 4. 0E-74 D87675. 1 NT Homo spaiens DNA for amyloid precursor protien, complete cds 842 5861 10901 5. 8 4. 0E-74 AB028942. 1 NT Homo sapiens mRNA for KIAA1019 protein, partial cds Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 1/2 (DLEC1, ORCTL3, ORCTL4 genes, 1922 6908 12002 1. 42 4. 0E-74 AB026898. 1 NT complete cds) Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 1/2 (DLEC1, ORCTL3, ORCTL4 genes, 1922 6908 12003 1. 42 4. 0E-74 AB026898. 1 NT complete cds) 2019 7002 12106 5. 12 4. 0E-74 4506192 NT Homo sapiens proteasome (prosome, macropain) subunit, beta type, 1 (PSMB1) mRNA Page 131 of 209 Table 4 Sinale Exon Probes Expressed in HBI. 100 Cells Most Similar Probe Exon ORF SEQ Expresioin (Top) Hit Top Hit Acession Top Hit SEQ ID I SEQ ID n : Signas BLAST E No. Source NO: NO: Source Value 2019 7002 12107 5.12 4.0E-74 4506192 NT Homo saplens proteasome (prosome, macropain) subunit, beta type,1 (PSMB1) mRNA 2074 7056 12166 1. 18 4. 0E-74 AB032994. 1 NT Homo sapiens mRNA for KIAA1168 protein, partial cds 3017 8034 13045 5. 03 4. 0E-74 AJ006976. 1 NT Homo sapiens PLP gene 3449 8457 13483 0. 81 4. 0E-74 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21 C010 3950 8948 13937 1. 22 4. 0E-74 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21 C047 4425 9415 14402 1.96 4. OE-74 7662183 NT Homo sapiens KIAA0569 gene product (KIAA0569), mRNA 4481 9471 14452 1. 19 4. 0E-74 Z17227. 1 NT Homo sapiens mRNA for transmebrane receptor protein Homo sapiens hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thidaselenoy-Coenzyme A 4902 9881 148501. 02 4. 0E-74 4504326 NT hydratase (trifunctional protein), beta subunit (HADHB) mRNA Homo sapiens hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A 4902 9881 14851 1. 02 4. 0E-74 4504326 NT hydratase (trifunctonal protein), beta subunit (HADHB) mRNA 5011 9982 14957 0. 91 4. 0E-74 AB037863. 1 NT Homo sapiens mRNA for KIAA1442 protein, partial cds 943 5960 10993 263. 61 2. OE-74 7669491 NT Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPD), mRNA 943 5960 10994 263. 61 2. OE-74 7669491 NT Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPD), mRNA 1156 6160 11194 1. 2 2. 0E-74 AF020092. 1 NT Human endogenous retrovirus HERV-K-T47D wx51e07.x1 NCI_CGAP_Lu28 Homo sapiens cDNA clone IMAGE : 2547204 3'similar to SW : GG95_HUMAN 1225 6224 11270 2. 76 2. 0E-74 AI950528. 1 EST HUMAN Q08379 GOLGIN-95. ; conins element MER22 repetitive element ; Homo sapiens epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene 1560 6557 11618 3. 33 2. OE-74 4885198 NT homolog) (EGFR) mRNA A Homo sapiens epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b) oncogene 1560 6557 11619 3.33 2. 0E-74 4885198 NT homolog) (EGFR) mRNA 2526 7492 12612 3. 99 2. 0E-74 AI557280. 1 EST HUMAN PT2. 1_15_G11.r tumor2 Homo sapiens cDNA 3' 4846 9828 14802 2. 77 2. 0E-74 AL355092. 1 NT Novel human gene mapping to chomosome 22 4846 9828 14803 2.77 2.0E-74 AL355092.1 NT Hovel human gene mapping to chomosome 22 4850 9832 14807 3. 98 2. 0E-74 J02963. 1 NT Human platelet glycoprotein IIb mRNA, 3' end 54 5135 10132 2. 92 1.0E-74 765734 NT Homo sapiens MisshapenlNIK-related kinase (MINK), mRNA 335 5387 10394 4.23 1. 0E-74 AW816405. 1 EST HUMAN QV4-ST0234-181199-037-f05 ST0234 Homo sapiens cDNA 496 5532 10539 1. 19 1. 0E-74 8922829 NT Homo sapiens hypothetical protein FLJ11026 (FLJ11026), mRNA 503 5538 10544 29. 75 1.0E-74 X02344. 1 NT Homo sapiens beta 2 gene 596562710626 2. 36 1. 0E-74 4508020 NT Homo sapiens zinc finger protein 259 (ZNF259) mRNA 984 5999 11030 2. 04 1. OE-74 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21C046 2165 7144 12262 5 1.0E-74 AB002059. 1 NT Homo sapiens DNA for Human P2XM, complete cds 3066 8082 13096 3. 19 1.0E--74 4758697 NT Homo sapiens mannosidase, alpha, class 2A, member 1 (MAN2A1), mRNA 3822 8824 13831 0. 67 1.0E-74 4504116 NT Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1) mRNA Page 132 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most similar Probe Exon Top Hit ORF SEQ Expresson (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor NO : NO : Value Source Value 3822 8824 13832 0. 67 1. 0E-74 4504116 NT Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1) mRNA 3861 8863 13868 4.97 1. OE-74 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21 C068 hz73h08. x1 NCI CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3213663 3' similar to WP:B0511. 12 4145 9140 14124 1.12 1. 0E-74 BE467769. 1 EST HUMAN CE17351 ; 2573 7536 3.52 8. OE-75 AF176228. 1 NT Homo sapiens DNA cytosin-5 methyttransferase 3B (DNMT3B) mRNA, complete cds wk38a08. x1 NCI CGAP_Pr22 Homo sapiens cDNA clone IMAGE : 2417654 3'similar to gb : M14123 cds4 2257 7234 12352 0. 98 6. 0E-75 AI817415. 1 EST_HUMAN RETROVIRUS-RELATED POLPOLYPROTEIN (HUMAN) ; 112 5184 10193 3.57 4. OE-75 BE081333. 1 EST HUMAN QV1-BT0632-210200-079-e02 BT0632 Homo sapiens cDNA 456 5493 0.99 4.0E-75 N36757. 1ESTHUMANyx90h08. r1 Spares metanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 269055 5' 1728 6723 11801 1. 27 4. 0E-75 AW897203, 1 EST HUMAN CMO-NN0057-150400-335-a11 NN0057 Homo sapiens cDNA 27767797 12816 5. 17 4.0E-75 BE409464. 1 EST_HUMAN 601303866F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3638344 5' 3421 8429 13455 0. 97 4. OE-75 8922637 NT Homo sapiens hypothetical protein FLJ10747 (FLJ10747), mRNA 987 6002 11033 2.28 3. OE-75 AF157623. 1 NT Homo sapiens HTRA serine protease (PRSS11) gene, complete cds 988 6002 11033 2.29 3.0E-75 AF157623.1 NT Homo sapiens HTRA serine protease (PRSS11) gene, complete cds 1800 6791 11881 1. 84 3. 0E-75 AB011153. 1 NT Homo sapiens mRNA for KIAA0581 protein, partial cds 1887 6876 11966 2. 26 3. OE-75 5453871 NT Homo sapiens platelet-derived growth factor receptor-like (PDGFRL) mRNA 2053 7035 12147 0. 92 3. 0E-75 4507334 NT Homo sapiens synaptojanin 1 (SYNJ1), mRNA 2356 7330 12446 3. 59 3. 0E-75 4759153 NT Homo sapiens synaptosomal-associated protein, 29kD (SNAP29) mRNA 2955 7974 12989 0.8 3.0E-75 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21C001 3116 8132 13151 1. 01 3.0E-75 AB011153. 1 NT Homo sapiens mRNA for KIAA0581 protein, partial cds 3273 8289 13308 0. 75 3. OE-75 M72393. 1 NT Human calcium-dependent phospholipid-binding protein (PLA2) mRNA, complete cds 3273 8285 13309 0. 75 3. OE-75 M72393. 1 NT Human calcium-dependent phospholipid-binding protein (PLA2) mRNA, complete cds 4044 9040 14031 1. 58 3. 0E-75 D87675. 1 NT Homo sapiens DNA for amyioid precursor protein, complete cds 4314 9306 14290 0.82 3. OE-75 7662421 NT Homo sapiens KIAA0971 protein (KIAA0971), mRNA 4996 9968 0. 82 3. OE-75 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21C009 xg60d02. x1 NCl CGAP_Ut4 Homo sapiens cDNA clone IMAGE : 2632707 3'similar to contains PTR7. t1 2239 7216 12334 30. 85 1. 0E-75 AW 168135. 1 EST HUMAN PTR7 repetitive element ; 2876 7895 12919 3.35 1.0E-75 X52221. 1 NT H. sapiens ERCC2 gene, exons 1 & 2 (partial) 4552 9540 14525 1.71 1.0E-75 BE279301. 1 EST HUMAN 601157633F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3504272 5' 4993 9965 14943 1. 23 1.0E-75 BE894192. 1 EST HUMAN 601437130F1 NIH MGC 72 Homo sapiens cDNA clone IMAGE : 3922303 5' wb30b10.x1 NCi_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2307163 3'similar to TR : 075235 075235 45 5126 10116 3. 62 9. 0E-76 AI652648. 1 EST HUMAN TRAP1 ; wb30b10. x1 NCtCGAPGC6 Homo sapiens cDNA clone IMAGE : 2307163 3 similar to TR : 075235 075235 45 5126 10117 3. 62 9. 0E-76 AI652648. 1 EST HUMAN TRAP1 ; Page 133 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells a.. -MostSimitar-.... Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No, Database Top Hit Descriptor Source NO : NO : Value 924 5941 10974 0. 84 8. OE-76 4504374 NT Homo sapiens H facta 1 (complement) (HF1) mRNA 924 5941 10975 0. 84 8. OE-76 4504374 NT Homo sapiens H factor 1 (complement) (HF1) mRNA Zg3g 7859 12879 1. 17 8. OE-76 7706724 NT Homo sapiens mediator (Sur2), mRNA Homo sapiens dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex, 2-oxo- 767 5788 10817 1. 86 7. OE-76 5016092 NT glutarate complex, branched chain kefo acid dehydrogenase complex) (DLD) mRNA 3220 8235 13256 3. 47 7. OE-76 AF056490. 1 NT Homo sapiens cAMP-specfic phosphodiesterase 8A (PDE8A) mRNA, partial cds 3226 8241 13263 7. 12 7. OE-76 4505052 NT Homo sapiens lymphocyte anfigen 75 (LY75) mRNA, and translated products 4246 9240 14223 4. 97 7. OE-76 4507184 NT Homo sapiens sepiapterin reductase (7, 8-dihydrobiopterin : NADP+ obdoreductase) (SPR) mRNA 4246 9240 14224 4. 97 7. OE-76 4507184 NT Homo sapiens sepiapterin reductase (7, 8-dihydrobiopterin : NADP+ obdoreductase) (SPR) mRNA 1214 6213 16. 64 6. OE-76 BE396253. 1 EST HUMAN 601312099F1 NIH_MGC 44 Homo sapiens cDNA clone IMAGE : 3658757 5' 1903 6890 11983 15. 24 S. OE-76 D63874. 1 NT Human mRNA for HMG-1, complete cds 1903 6890 11984 15. 24 5. OE-76 D63874. 1 NT Human mRNA for HMG-1, complete cds' 1903 6890 11985 15. 24 5. OE-76 D63874. 1 NT Human mRNA for HMG-1, complete cds 3134 8150 13172 0. 8 4. OE-76 BE814096. 1 EST HUMAN QV3-BN0047-270700-283-g06 BN0047 Homo sapiens cDNA 623 5650 10653 1. 54 3. OE-76 BF516262. 1 EST_HUMAN UI-H-BW 1-anz b-04-0-Ui. s1 NCI CGAP Sub7 Homo sapiens cDNA clone IMAGE : 3083862 3' 623 5650 10654 1. 54 3. OE-76 BF516262. 1 EST HUMAN UI-H-BW1-anz-b-04-0-UI. s1 NCI CGAP Sub7 Homo sapiens cDNA clone IMAGE : 3083862 3' 1564 6561 11623 21. 41 3. OE-76 4503476 NT Homo sapiens eukaryotic transla6on elongation factor 1 beta 2 (EEF182) mRNA 1564 6561 11624 21. 41 3. OE-76 4503476 NT Homo sapiens eukaryoGc translation elonga6on factor 1 beta 2 (EEF1 B2) mRNA 3344 8353 13370 6. 25 3. OE-76 BF375689. 1 EST HUMAN RC5-ST0300-180100-033-A03 ST0300 Homo sapiens cDNA 3344 8353 13371 6. 25 3. OE-76 BF375689. 1 EST HUMAN RC5-ST0300-180100-033-A03 ST0300 Homo sapiens cDNA 279 5337 10351 1 2. OE-76 D84295. 9 NT Human mRNA for possible protein TPRDII, complete cds 340 5392 10398 1. 94 2. OE-76 D84295. 1 NT Human mRNA for possible protein TPRDII, complete cds 340 5392 10399 1. 94 2. OE-76 D84295. 1 NT Human mRNA for possible protein TPRDII, complete cds 458 5495 1. 42 2. OE-76 4557662 NT Homo sapiens immunoglobulin (CD79A) binding protein 1 (IGBP1) mRNA 585 5616 10615 1. 68 2. OE-76 4503944 NT Homo sapiens glucagon (GCG) mRNA 9094 6024 11056 1. 16 2. OE-76 4758053 NT Homo sapiens cAMP responsive element binding protein 1 (CREB1) mRNA 1504 6502 11558 2. 34 2. OE-76 4504028 NT Homo sapiens GM2 ganglioside activata protein (GM2A) mRNA 1504 6502 11559 2. 34 2. OE-76 4504028 NT Homo sapiens GM2 ganglioside activator protein (GM2A) mRNA 2768 7789 12811 3. 35 2. OE-76 P23266 SW ISSPROT OLFACTORY RECEPTOR-LIKE PROTEIN F5 zw64e02. s1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 780986 3'similar to SW : ITB5 HUMAN 3223 8238 13260 1. 89 2. OE-76 AA445992. 1 EST HUMAN P18084 INTEGRIN BETA-5 SUBUNIT PRECURSOR. ; zw64e02. s1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 780986 3'similar to SW : ITB5_HUMAN 3223 8238 13261 1. 89 2. OE-76 AA445992. 1 EST HUMAN P18084 INTEGRIN BETA-5 SUBUNIT PRECURSOR. ; Page 134 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ W SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. Value sc83b02. y5 Stratagene lung (#937210) Homo sapiens cDNA clone MAGE : 869163 5'similar to TR : 014591 3396 8404 13430 1.04 2.0E-76 Al821149. 4EST_HUMAN 1 EST HUMAN 014591 SIMILARITY TO P22059 ; zu70g11. r1 SoarestestisNHT Homo sapiens cDNA clone IMAGE : 743396 5 similar to WP : R05D3. 2 3682 8686 13688 7. 33 2. OE-76 AA400700. 1 9391 14375 1 2.0E-76 AL163283:2 NT Homo CE00281 ; 4400 9774 14758 6.31 20E-76 AW879618. sapiens chromosome 21 segment HS21C083 4790 EST-HUMAN QV3-OT0028-220300-132-b11 OT0028 1 187 5250 10261 3.03 8.0E-77 Homo sapiens cDNA 4173 9168 14155 5. 78 1. OE-76 D63874. 1 NT Human mRNA for HMG-1, complete cds 4173 9168 14156 5. 78 1. OE-76 D63874. 1 NT. Human mRNA for HMG-1, complete cds yp1 h02. r1 Soares breast 3NbHBst Homo sapiens cDNA clone IMAGE : 187155 5'similar to EST_HUMAN 601866926F1 NIH_MGC_17 R83144. 1 EST HUMAN SP : ANKB HUMAN Q01484 ANKYRIN, BRAiN VARIANT 1 ; 4391 9382 14364 1. 16 8. OE-77 BF205181. 1 (RNA) II Homo sapiens cDNA clone IMAGE : 4109503 5' 1889 6878 11968 1. 52 7. OE-77 AA625755. 1 EST HUMAN zu91g01. s1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 745392 3' 2345 7319 12439 9. 62 7. OE-77 4505944 NT Homo sapiens polymerase 2345 7319 12440 9.62 7.0E-77 4505944 NT Homo (DNA directed) polypeptide E (25kD) (POLR2E) mRNA polypeptide sapiens polymerase (RNA) II (DNA directed) 1511 6509 11566 2.09 6.0E-77 AI204066. E (25kD) (POLR2E) mRNA 260 5319 10329 5. 1 6. OE-77 4504600 NT Homo sapiens interferon (alpha, beta and omega) receptor 2 (IFNAR2) mRNA EST_HUMAN qe77h12.x1 soares-fetal_lung_NbHL19W 1 (MID1) mRNA Homo sapiens cDNA clone IMAGE : 1745063 3' 4772 9756 14743 0. 98 6. OE-77 4557752 NT Homo sapiens midline 1 (Opitz/BBB syndrome) (MID1) mRNA 4772 9756 14744 0. 98 6. OE-77 4557752 NT Homo sapiens midline 1 (Opitz/BBB syndrome) 1216 6215 11255 1.5 5.0E-77 AF041015. 1343 6340 11391 2.76 5.0E-77 1 NT 7 Homo sapiens glucokinase (GCK) gene, exon 2 2613 7575 12689 1.11 5.0E-77 AF162666. 4557250 NT Homo sapiens disintegrin and metalloprotease domain 10 (ADAM10) mRNA 3443 8451 13478 1.75 5.0E-77 8394518 1 NT Homo sapiens tousled-like kinase 1 (TLK1) mRNA, complete cds 2690 7648 12762 24 5. OE-77 4503160 NT Homo sapiens cullin 1 (CUL1) mRNA I-like domains 3 (EDIL3), mRNA NT Homo sapiens ubiquitin specific protease 18 (USP18), mRNA 4565 9553 14539 0. 99 5. OE-77 5031660 NT Homo sapiens EGF-like repeats and discoidin (EDIL3), 4565 9553 14540 0. 99 5. OE-77 5031660 NT Homo sapiens EGF-like repeats and discoidin I-like domains 3 66 5.0E-77 AL043953.1 EST_HUMAN DKFZp434G1728_r1 mRNA 4780 9764 14749 2. DKFZp434G1728 5' 434 (synonym : htes3) Homo sapiens cDNA clone 8627 13634 3620 09 1. 93 4. OE-77 AL449758. 1 EST HUMAN AL449758 Homo sapiens fetal brain (Stavrides GS) Homo sapiens cDNA 1928 6914 12010 1. 57 3. OE-77 5730038 NT Homo sapiens SET domain and mariner transposase fusion gene (SETMAR) mRNA 1928 6914 12011 1. 57 3. OE-77 5730038 NT Homo sapiens SET domain and mariner transposase fusion gene (SETMAR) mRNA 1334 6332 11380 2. 2.0E-77 AV764617. 1 EST-HUMAN AV764617 MDS Homo sapiens cDNA clone MDSBTF10 5' 1407 6405 11464 7. 17 2. OE-77 AW997712. 1 EST HUMAN RC3-BN0053-170200-011-h01 BN0053 Homo sapiens cDNA 2045 7027 12138 5. 42 2.0E-77 7706315 NT Homo sapiens CGI-79 protein (LOC51634), mRNA 2518 7773 12602 2. 22 2.0E-77 AB037836. 1 NT Homo sapiens mRNA for KIAA1415 protein, partial cds 2518 7773 12603 2. 22 2.0E-77 AB037836. 1 NT Homo sapiens mRNA for KiAA1415 protein, partial cds Page 135 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe @ Exon Most Similar Top Hit Prob Exon ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. source Value ho43b05. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 3040113 3'similar to 3922 8922 13813 1. 53 2. 0E-77 BE044316. 1 EST HUMAN SW : GAG2_HUMAN P10264 RETROVIRUS-RELATED GAG POLYPROTEIN ; tw22g02. x1 NCI CGAP Brn52 Homo sapiens cDNA clone IMAGE : 2260466 3'similar to TR : 065245 4288 9280 14268 0. 82 2. OE-77 AI613519 1 EST HUMAN 065245 F21E10. 7 PROTEIN. ; tw22g02. x1 NCI CGAP Brn52 Homo sapiens cDNA clone IMAGE : 2260466 3'similar to TR : 065245 4288 9280 14269 0. 82 2. OE-77 AI613519. 1 EST HUMAN 065245 F21E10. 7 PROTEIN. ; Homo sapiens glutamic-oxalocacetic transaminase 2, mitochondrial (aspartate aminotransferase 2) (GOT2), 4465 9455 1. 29 2. OE-77 4504068 NT nuclear gene encoding mitochondrial protein, mRNA ns68g12.s1 NCI_CGAP_Pr2 Homo sapiens cDNA clone IMAGE : 1188838 similar to SW : RU29 HUMAIN 4623 9608 14596 6. 49 2. OE-77 AA653025. 1 EST HUMAN P47914 60S RIBOSOMAL PROTEIN L29. [1] ; contains element MSR1 repetitive element ; 44 5124 10112 0. 89 1. 0E-77 AB033102. 1 NT Homo sapiens mRNA for KIAA1276 protein, partial cds 44 5124 10113 0. 89 1. 0E-77 AB033102. 1 NT Homo sapiens mRNA for KIAA1276 protein, partial cds 270 5329 10340 3. 11 1. OE-77 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) (APP), mRNA 270 5329 10341 3. 11 1. OE-77 4502166 NT Homo sapiens amyioid beta (A4) precursor protein (protease nexin-ii, Alzheimer disease) (APP), mRNA 864 7733 10925 6.08 1. OE-77 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) (APP), mRNA 864 7733 10926 6. 08 1. 0E-77 4502166 NT Homo sapiens amyioid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) (APP), mRNA 1875 6864 11953 1.58 1.0E-77 AW058119. 1 EST HUMAN wv83e05. xi Soares thymus_NHFTh Homo sapiens cDNA clone IMAGE : 2536160 3' 2376 7348 12469 1.33 1.0E-77 AB029024. 1 NT Homo sapiens mRNA for KIAA1101 protein, complete cds 2971 7989 13002 2.25 1.0E-77 4503300 NT Homo sapiens 2, 4-dienoyl CoA reductase 1, mitochondrial (DECR1), mRNA 4227 9221 14201 4. 08 1. OE-77 7706299 NT Homo sapiens CGI-60 protein (LOC51626), mRNA 4393 9384 14366 17. 29 1. OE-77 AJ229041. 1 NT Homo sapiens 959 kb contig between AML1 and CBR1 on chromosome 21 q22 ; segment 1/3 4516 9506 14486 2. 27 1. OE-77 6552322 NT Homo sapiens breast cancer 1, early onset (BRCA1), transcript variant BRCA1-exon4, mRNA 4554 9542 14527 0.74 1.0E-77 AI273014. 1 EST_HUMAN qv09g04.x1 NCI_CGAP_Kid8 Homo sapiens cDNA clone IMAGE : 1981110 3' 4728 9713 14698 1.24 1.0E-77 11418424 NT Homo sapiens collagen, type XII, alpha 1 9COL12A1), mRNA 4898 9877 14845 1. 42 1. OE-77 7661849 NT Homo sapiens KIAA0005 gene product (KIAA0005), mRNA 4898 9877 14846 1.42 1.0E-77 7661849 NT Homo sapiens KIAA0005 gene product (KIAA0005), mRNA 4989 9361 14341 0. 68 1. OE-77 4758053 NT Homo sapiens cAMP responsive element binding protein 1 (CREB1) mRNA 84 5161 10172 2. 26 6. OE-78 AU118789. 1 EST HUMAN AU118789 HEMBA1 Homo sapiens cDNA clone HEMBA1004354 5' 84 5161 10173 2. 26 6. 0E-78 AU118789. 1 EST HUMAN AU118789 HEMBA1 Homo sapiens cDNA clone HEMBA1004354 5' Page 136 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit Probe Exon @@@@@@ @@@@@@ Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value 3240 8253 13274 0.88 6.0E-78 BF344101. 1 EST_HUMAN 602016926F1 NCI_CGAP_Brn64 Homo sapiens cDNA clone IMAGE : 4152511 5' 217 5280 10291 1.01 5.0E-78 11422486 NT Homo sapiens hypothetical protein FLJ11316 (FLJ11316), mRNA ba54h03. y3 NIH_MGC_10 Homo sapiens cDNA clone IMAGE : 2900405 5'similar to WP : Y48B6A. 6 2491 7459 12574 4. 77 5. OE-78 AW673424. 1 EST HUMAN CE22121 ; 3301 8312 13339 4. 18 5. OE-78 M55586. 1 NT Human collagenase type IV (CLG4) gene, exon 6 1120 6126 11156 1. 66 4. OE-78 AL043314. 2 EST HUMAN DKFZp434N0323 r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434N0323 5' 1487 6484 11539 1.28 4.0E-78 AL355841.1 NT Novel human gene mapping to chomosome 22 2254 7231 12350 20. 59 4. OE-78 AF107405. 1 NT Homo sapiens pre-mRNA splicing factor (SFRS3) mRNA, complete cds 4195 9188 14168 1.87 4.0E-78 7656876 NT Homo sapiens syncytin (LOC30816), mRNA 4628 9613 14602 1. 75 4. OE-78 4505806 NT Homo sapiens phosphatidyiinositol 4-kinase, catalytic, alpha polypeptide (PIK4CA) mRNA 4628 9613 14603 1.75 4.0E-78 4505806 NT Homo sapiens phosphatidytinositd 4-kinase, catalytic, alpha polypeptide (PIK4CA) mRNA 160 5226 10235 3. 27 3. 0E-78 AF095901. 1 NT Homo sapiens eRF1 gene, complete cds 160 5226 10236 3. 27 3.0E-78 AF095901. 1 NT Homo sapiens eRF1 gene, complete cds 2240 7217 12335 1. 06 3. OE-78 4502142 NT Homo sapiens apoptosis inhibitor 3 (API3) mRNA 3142 8158 13180 1. 34 3. OE-78 4507164 NT Homo sapiens nuclear antigen Sp100 (SP100) mRNA 3678 8683 1.23 3.0E-78 AU140604. 1 EST_HUMAN AU140604 PLACE3 Homo sapiens cDNA clone PLACE3000373 5' 3048 8065 2. 47 2. OE-78 U04489. 1 NT Homo sapiens type IV collagen alpha 5 chain (COL4A5) gene, exon 20 3907 8907 1. 51 2. OE-78 AA311872. 1 EST HUMAN EST182583 Jurkat T-cells Vi Homo sapiens cDNA 5'end 5007 9978 14953 1.22 1.0E-78 4758843 NT Homo sapiens nucleoporin 155kD (NUP155) mRNA 4560 9548 14533 3. 65 9. OE-79 11525891 NT Homo sapiens peptideYY (PYY), mRNA 4714 9699 14684 5. 64 9. OE-79 BE000837. 1 ESTHUMAN RC2-BN0074-090300-014-c12BN0074 Homo sapiens cDNA 3657 8663 13668 0. 91 8. OE-79 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 4366 9357 14337 1.57 8.0E-79 D28476.1 NT Human mRNA for KIAA0045 gene, complete cds 4366 9357 14338 1.57 8.0E-79 D28476.1 NT Human mRNA for KIAA0045 gene, complete cds 3179 8195 13218 19. 1 7.0E-79 BE619648. 1 EST HUMAN 601472766T1 NIH MGC 68 Homo sapiens cDNA clone IMAGE : 3875657 3' 3103 8119 0. 99 4. OE-79 8922325 NT Homo sapiens hypothetical protein FLJ10283 (FLJ10283), mRNA 311 5366 10377 1. 61 3.0E-79 AF114488. 1 NT Homo sapiens intersectin short isofo 963 5978 11012 5. 56 3. OE-79 AF232708. 1 NT Homo sapiens cell-line tsA201a chloride ion current inducer protein (Cln) gene, complete cds 3025 8042 13051 2. 52 3.0E-79 U09410. 1 NT Human zinc finger protein ZNF131 mRNA, partial cds 285 5343 1. 02 2. OE-79 H63129. 1 EST HUMAN yr48f03. s1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 208541 3' 628 5656 10661 1.22 2.0E-79 BE379926. 1 EST_HUMAN 601159415F2 NIH-MGC_53 Homo sapiens cDNA clone IMAGE : 3511107 5' 915 5931 10966 1. 47 2.0E-79 4757841 NT Homo sapiens BCL2-like 2 (BCL2L2) mRNA 1018 6028 1. 19 2. OE-79 AI523747. 1 EST HUMAN th18h07. x1 NCI CGAP_Pr28 Homo sapiens cDNA clone IMAGE : 2118685 3' 1751 6745 11824 0. 92 2. OE-79 7657024 NT Homo sapiens Dickkopf gene 4 (DKK-4), mRNA Page 137 of 209 Table 4 Sinqle Exon Probes Exaressed in HBL100 Cells Probe Exon Most Similar Top Hit robe NO: NO: ID NO: Signal BLAST E No. Source Value 1751 6745 11825 0.92 2.0E-79 7657024 NT Homo sapiens Dickkopf gene 4 (DKK-4), mRNA 2087 7068 12180 3. 31 2.0E-79 4585863 NT Homo sapiens phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), mRNA 2087 7068 12181 3. 3 2. OE-79 4585863 NT Homo sapiens phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), mRNA 2130 7110 12223 2. 05 2. OE-79 AJ271408. 1 NT Homo sapiens mRNA for Fas-associated factor, FAF1 (Faf1 gene) 2249 7226 12346 2. 65 2. OE-79 AF244138. 1 NT Homo sapiens hepatocellular carcinoma-associated antigen 88 (HCA88) mRNA, complete cds 4041 9037 14027 1. 34 2. 0E-79 AJ271408. 1 NT Homo sapiens mRNA for Fas-associated factor, FAF1 (Faf1 gene) 3073 8089 13102 18. 41 9. OE-80 AA725848. 1 EST_HUMAN ai23e05.s1 Soares_testis-NHT Homo sapiens cDNA clone 1343648 3' 3073 8089 13103 18. 41 9. OE-80 AA725848. 1 EST HUMAN ai23e05. s1 Soares testis NHT Homo sapiens cDNA clone 1343648 3' 3521 8528 1.06 8.0E-80 U94387. 1 NT Homo sapiens Y chromosome spermatogenesis candidate protein (RBM) pseudogene mRNA, partial cds 4797 9781 14764 1. 67 7. OE-80 H04619. 1 EST HUMAN yj49d02. r1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 152067 5' tf58d02. x1 NCI CGAP Brn23 Homo sapiens cDNA clone IMAGE : 2103459 3'similar to SW : NUEMHUMAN 889 5907 10947 2.22 6.0E-80 AI422197. 1 EST_HUMAN Q16795 NADH-UBIQUINONE OXIDOREDUCTASE 39 KD SUBUNIT PRECURSOR ; 1604 6600 11661 2. 05 6. OE-80 U64898. 1 NT Homo sapiens NRD convertase mRNA, complete cds 2234 7211 12327 4. 78 6. OE-80 6631094 NT Homo sapiens minichromosome maintenance deficient (S. cerevisiae) 3 (MCM3), mRNA 2234 7211 12328 4. 78 6. OE-80 6631094 NT Homo sapiens minichromosome maintenance deficient (S. cerevisiae) 3 (MCM3), mRNA 4162 9157 14142 1. 33 6. OE-80 AB032981. 1 NT Homo sapiens mRNA for KIAA1155 protein, partial cds 4162 9157 14143 1. 33 6. OE-80 AB032981. 1 NT Homo sapiens mRNA for KIAA1155 protein, partial cds 583 5614 10614 5. 48 5. OE-80 4506228 NT Homo sapiens proteasome (prosome, macropain) 26S subunit, non-ATPase, 3 (PSMD3) mRNA 825 5845 10882 1. 83 5. OE-80 AF108830. 1 NT Homo sapiens serine-threonine protein kinase (MNBH) mRNA, complete cds 825 5845 10883 1. 83 5. OE-80 AF108830. 1 NT Homo sapiens serine-threonine protein kinase (MNBH) mRNA, complete cds 1165 6172 1. 23 5. 0E-80 X91647. 1 NT H. sapiens ncx1 gene (exon 12) 1429 6426 1.63 5.0E-80 AL163283. 2 NT Homo sapiens chromosome 21 segment HS21 C083 2300 7275 12394 1. 18 5. OE-80 U89358. 1 NT Human 1 (3) mbt protein homolog mRNA, complete cds 2363 7337 12454 8. 73 5. 0E-80 AB037855. 1 NT Homo sapiens mRNA for KIAA1434 protein, partial cds 2722 7679 12792 6. 29 5. OE-80 4504292 NT Homo sapiens H3 histone family, member J (H3FJ) mRNA 4796 9780 14763 1. 24 5. OE-80 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21 C068 216 5729 15.21 3.0E-80 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 4571 9559 14548 1.41 3. 0E-80 BF085009. 1 ESTHUMAN PMO-GN0018-040900-002-E03 GN0018 Homo sapiens cDNA 4752 9738 7 56 3. 0E-80 BE817465. 1 EST_HUMAN QV4-BN0263-040600-241-g10 BN0263 Homo sapiens cDNA 1762 6754 11839 3.95 2.0E-80 R35321. 1 EST HUMAN yg65a08. r1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 38060 5' 1823 68131 119071 1. 48 2.0E-80 AI444821.1 EST_HUMAN RET4B7 subtracted retina cDNA library Homo sapiens cDNA clone RET4B7 2002 6985 12089 3. 58 2.0E-80 AL,043116. 2 EST HUMAN = = t Page 138 of 209 Tabfe 4 Sinale Exon Probes Exaressed in HBL10 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID NO : NO: ID NO: Signal BLAST E No. top Hit Descriptor Value 338 5390 1.79 1.0E-80 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 791 5812 10842 1. 53 1. OE-80 AF231920. 1 NT Homo sapiens chromosome 21 unknown mRNA nn01f12. x5 NCl_CGAP_Co9 Homo sapiens cDNA clone IMAGE : 1076495 3'similar to contains OFR. t1 OFR 1914 6900 2. 27 1. OE-80 Al732656. 1 EST HUMAN repetitive element ; za39g07.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 294972 5'similar to contains 4674 9659 14642 0. 85 1. OE-80 N99520. 1 EST HUMAN Alu repetitive element ; ze21d10. r1 Soares_fetal_heart_NbHH19W Homo sapiens cDNA clone IMAGE : 359635 5'similar to SW : KRHA_RABIT @ Q02957 KERATIN, GLYCINE/TYROSINE-RICH OF HAIR. [1] ; contains element MER22 2144 7123 12239 0. 95 7. 0E-81 AA011080. 1 EST_HUMAN repetitive element ; 4262 9255 14244 6. 63 6. 0E-81 BE256829. 1 EST-HUMAN 601111970F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE : 3352840 5' 4262 9255 14245 6. 63 6. 0E-81 BE256829. 1 EST HUMAN 601111970F1 NIH MGC_16 Homo sapiens cDNA clone IMAGE : 3352840 5' 2155 7134 12253 5. 16 5. OE-81 BE268042. 1 EST_HUMAN 601125505F1 NIH_MGC_8 Homo sapiens cDNA clone IMAGE : 3345480 5' hn98d02. x1 NC) CGAPCo14 Homo sapiens cDNA clone IMAGE : 3035907 3'similar to SW : COPS BOVIN 1786 6778 11870 1. 6 4. 0E-81 AW779612. 1 ESTHUMAN P53620COATOMER GAMMA SUBUNIT ; 3097 8113 13131 3.66 4.0E-81 AB037766.1 NT Homo sapiens mRNA for KIAA1345 protei, pertial cds ws90h03.x1 NCI_CGAP_Co3 Homo sapiens cDNA clone IMAGE:2506269 3' similar to TR;O43815 O43815 3544 8551 13558 0. 78 4. 0E-81 AW004608. 1 EST-HUMAN STRIATIN- ; 4036 9032 14019 2. 04 4. 0E-81 AF263306. 1 NT Homo sapiens rab3 interacting protein variant 2 mRNA, partial cds 4036 9032 14020 2. 04 4. 0E-81 AF263306. 1 NT Homo sapiens rab3 interacting protein variant 2 mRNA, partial cds 4268 9261 14251 0. 99 4. 0E-81 8923209 NT Homo sapiens hypothetical protein FLJ20220 (FLJ20220), mRNA 1248 6246 11285 12. 27 3. 0E-81 Y18000. 1 NT Homo sapiens NF2 gene 1248 6246 11286 12. 27 3. 0E-81 Y18000. 1 NT Homo sapiens NF2 gene 2310 7285 12405 1. 5 3. 0E-81 AF077188. 1 NT Homo sapiens cullin 4A (CUL4A) mRNA, complete cds 2922 7941 12957 5. 68 3. 0E-81 4506280 NT Homo sapiens pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) (PTN) mRNA 2922 7941 12958 5. 68 3. 0E-81 4506280 NT Homo sapiens pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1) (PTN) mRNA 2759 7780 12804 2. 23 2. 0E-81 BE784636. 1 EST_HUMAN 601474072F1 NIH_MGC_68 Homo sapiens cDNA clone IMAGE : 3877121 5' 2759 7780 12805 2. 23 2. 0E-81 BE784636. 1 EST_HUMAN 601474072F1 NIH_MGC_68 Homo sapiens cDNA clone IMAGE : 3877121 5' 3686 8690 13693 0. 93 2. 0E-81 AW611542. 1 EST HUMAN hg85cO1. x1 NCI CGAP Kid11 Homo sapiens cDNA clone IMAGE : 2952384 3' 3568 8575 13581 1. 35 1. 0E-81 AW960658. 1 EST HUMAN EST372729 MAGE resequences, MAGF Homo sapiens cDNA zk45h09. r1 Soarespregnant uterus_NbHPU Homo sapiens cDNA clone IMAGE : 485825 5'similar to 4384 9375 14354 2. 11 1. 0E-81 AA040370. 1 EST HUMAN PIR : S52437 S52437 CDP-diacylgiycerd synthase-fruitfly ; 4510 9500 14479 10. 97 1. 0E-81 BE047996. 1 EST HUMAN tz45c04. y1 NCI CGAP Brn52 Homo sapiens cDNA clone IMAGE : 2291526 5' Page 139 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Epxressoin (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLASTE No. Database Top Hit Descriptor NO : NO : Value Source 13 6093 10077 4. 69 8.0E-82 AF161406. 1 NT Homo sapiens HSPC288 mRNA, partial cds 1075093 100775. 268. 0E-82 AF161406. 1 NT Homo sapiens HSPC288 mRNA, partial cds 261 5320 10330 2. 55 8.0E-82 U08988.1 NT Human CRFB4 gene, partial cds 804 5825 10855 2. 38 8. OE-82 U08988. 1 NT Human CRFB4 gene, partial cds 876 5894 10935 1. 93 8. 0E-82 U08988. 1 NT Human CRFB4 gene, partial cds 1459 6456 11515 1. 39 8.0E-82 AB037748. 1 NT Homo sapiens mRNA for KIAA1327 protein, partial cds Homo sapiens glutathione peroxidase 5 (epididymal androgen-related protein) (GPX5), transcript variant 2, 1617 6614 11681 1.23 8.0E-82 6715601 NT mRNA 4121 9115 14102 0. 84 8. OE-82 8923432 NT Homo sapiens hypothetical protein FLJ20461 (FLJ20461), mRNA 1424 6421 1.04 7.0E-82 BF035327. 1 EST_HUMAN 601458531F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3862086 5' 2693 7651 12765 1. 55 7.0E-82 AU144050. 1 EST HUMAN AU144050 HEMBA1 Homo sapiens cDNA clone HEMBA1000752 3' 1632 6629 11698 100. 34 4. OE-82 AF081484. 1 NT Homo sapiens alpha-tubulin isoform 1 mRNA, complete cds 275 5334 10347 16. 31 3. OE-82 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) (APP), mRNA 693 5717 10734 3. 55 3. OE-82 BE005705. 1 EST-HUMAN RC2-BN0120-010400-013-f02 BN0120 Homo sapiens cDNA 779 5800 10830 5.3 3.0E-82 514702 NT Homo sapiens transforming growth factor beta-activated kinase-binding protein 1 (TAB1), mRNA 859 587 10919 8. 88 3. 0E-82 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nexin-II, alzheimer disease) (APP), mRNA 1044 6054 90.59 3.0E-82 AA725848. 1 EST-HUMAIN ai23e05. s1 SoarestestisNHT Homo sapiens cDNA clone 1343648 3' 1337 6335 11385 0.96 3.0E-82 AW875073.1 EST_HUMAN RC6-PT0001-190100-021-B02 PT0001 Homo sapiens cDNA 1438 6435 11492 2. 02 3. OE-82 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 18606849 11937 1. 66 3. OE-82 BE813232. 1 ESTHUMANRC1-BNOOOS-260700-018-g04BN0005 Homo sapiens cDNA 3198 8214 2.15 3.0E-82 5453811 NT Homo sapiens neurotrophic tyrosine kinase, receptor, type 2 (NTRK2) mRNA 592 562'10621 1. 39 20E-82 AB023216. 1 NT Homo sapiens mRNA for KIAA0999 protein, partial cds 592 5623 10622 1. 39 2. OE-82 AB023216. 1 NT Homo sapiens mRNA for KIAA0999 protein, partial cds 1647 6643 11715 1. 78 2. 0E-82 AL046390. 1 EST HUMAN DKFZp434M117 r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434M117 5' 3756 8759 13758 0. 85 2.0E-82 D87675. 1 NT Homo sapiens DNA for amyloid precursor protein, complete cds 4112 9106 14092 0. 83 2.0E-82 4504116 NT Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1) mRNA 4426 9416 14403 1. 06 2. OE-82 AB029019. 1 NT Homo sapiens mRNA for KIAA1096 protein, partial cds 4426 9416 14404 1.06 2.0E-82 AB029019. 1 NT Homo sapiens mRNA for KIAA1096 protein, partial cds Homo sapiens wbscr1 (WBSCR1) and wbscr5 (WBSCR5) genes, complete cds, alternatively spliced and 4720 9705 14691 2. 77 2.0E-82 AF045555. 1 VINT replication factor C subunit 2 (RFC2) gene, complete cds 4908 9887 14859 1.58 2.0E-82 4507580 NT Homo sapiens tumor necrosis factor receptor superfamily, member 5 (TNFRSF5) mRNA 4908 9887 14860 1. 58 2. OE-82 4507580 NT Homo sapiens tumor necrosis factor receptor superfamily, member 5 (TNFRSF5) mRNA Page 140 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Too Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession TaP Hit Descriptor NO NO: Signal BLAST E No. Database Top Hit Descriptor Value 587 5618 10616 1. 67 1. OE-82 11545921 NT Homo sapiens melanoma differentiation associated protein-5 (MDA5), mRNA 1189 6190 1. 57 1. OE-82 BE885106. 1 EST HUMAN 601510859F1 NIH MGC_71 Homo sapiens cDNA clone IMAGE : 3912207 5' 1266 6264 11305 3.2 1.0E-82 BE064386. 1 EST_HUMAN RC4-BT0310-110300-015-f10 BT0310 Homo sapiens cDNA 1267 6265 11306 1. 14 1. 0E-82 AB011110. 2 NT Homo sapiens mRNA for KIAA0538 protein, partial cds 1389 6386 11438 4. 62 8. 0E-83 BE383973. 1 EST_HUMAN 601273346F1 NIH_MGC_20 Homo sapiens cDNA clone IMAGE : 3614362 5' 1642 7700 11709 4. 3 8.0E-83 N66951. 1 EST HUMAN za48f12. s1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 295823 3' 1339 6337 11386 1. 67 7. OE-83 AW385529. 1 EST HUMAN QV4-LT0016-271299-068-h11 LT0016 Homo sapiens cDNA no12h01. s1 NCI_CGAP_Phe1 Homo sapiens cDNA clone IMAGE : 1100497 3'similar to contains Alu 2794 7814 1. 62 7. 0E-83 AA584655. 1 EST_HUMAN repetitive element ; 7p37a07. x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE : 3647893 3'similar to TR : Q9Y316 Q9Y316 4670 9655 6. 92 7. 0E-83 BF221813. 1 ESTHUMAN DJ207H1. 1 ; 7p37a07. x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE : 3647893 3'similar to TR : Q9Y316 Q9Y316 5036 10007 1. 51 7. 0E-83 BF221813. 1 ESTHUMAN DJ207H1. 1 ; 401 5437 10456 1. 97 6. 0E-83 M33320. 1 NT Human platelet Glycoprotein llb (GPllb) gene, exons 2-29 hf31h03. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2933525 3'similar to 1749 6743 11822 7. 97 6. 0E-83 AW573088. 1 EST HUMAN SW : YBEB HAEIN P44471 HYPOTHETICAL PROTEIN HI0034, ; 2977 7995 1. 02 6. 0E-83 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 2996 8014 13026 1. 01 6. 0E-83 AA701457. 1 EST_HUMAN zi59xc05.s1 Soares_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 435080 3' 3483 8491 13507 0. 82 6. 0E-83 11430241 NT Homo sapiens hypothetical protein FLJ10379 (FLJ10379), mRNA 932 5949 1. 95 5. 0E-83 U17883. 1 NT Human succinate dehydrogenase iron-protein subunit (sdhB) gene, exon 5 1997 7704 2. 85 5. OE-83 AF006305. 1 NT Homo sapiens 26S proteasome regulatory subunit (SUG2) mRNA, complete cds 3553 8560 13566 1. 13 5. 0E-83 AL133207. 2 NT Novel human gene mapping to chomosome X 3810 8813 13819 0.99 5.0E-83 4885190 NT Homo sapiens deoxyribonuclease I (DNASE1), mRNA 4907 9886 14857 14. 02 5. 0E-83 4557013 NT Homo sapiens catalase (CAT) mRNA 4907 9886 14858 14. 02 5. 0E-83 4557013 NT Homo sapiens cataiase (CAT) mRNA Homo sapiens mannosidase, beta A, lysosomal (MANBA) gene, and ubiquitin-conjugating enzyme E2D 3 634 5662 10667 1. 76 4. 0E-83 AF224669. 1 NT (UBE2D3) genes, complete cds 3435 8443 13469 1. 08 4. 0E-83 BE888078. 1 EST HUMAN 601511580F1 NIH MGC 71 Homo sapiens cDNA clone IMAGE : 3913195 5' 982 5997 3. 79 3. 0E-83 AA368311. 1 EST-HUMAN EST79542 Placenta I Homo sapiens cDNA similar to similar to endogenous retrovirus ERV9 np87c07. s1 NCI_CGAP_Thy1 Homo sapiens cDNA clone IMAGE : 1133292 similar to contains THR. t2 THR 2705 7662 1. 44 3. 0E-83 AA632654. 1 EST HUMAN repetitive element ; ot64g05. s1 SoarestestisNHT Homo sapiens cDNA clone IMAGE : 1621592 3'similar to TR : Q92614 1764 6756 11841 1.7 2.0E-83 AA993492.1 EST-HUMAN Q92614 MYELOBLAST KIAA0216. ; Page 141 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expressin (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BALST E No. NO: NO: Source Valut ot64g05. s1 SoarestestisNHT Homo sapiens cDNA clone IMAGE : 1621592 3'similar to TR : Q92614 1764 6756 11842 1. 7 2. 0E-83 AA93492. 1 EST HUMAN Q92614 MYELOBLAST KIAA0216. ; 1884 6873 11962 3. 01 2. 0E-83 N66951. 1 EST-HUMAN za48fl2. sl Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 295823 3' 2779 7800 12818 2. 73 2. 0E-83 BE828694. 1 ESTHUMAN RC6-ET0046-280600-013-H12 ET0046 Homo sapiens cDNA 3195 8211 2. 22 2. 0E-83 11430834 NT Homo sapiens sal (Drosophila)-like 1 (SALL1), mRNA 3687 8691 0. 74 2. 0E-83 AL163202. 2 NT Homo sapiens chromosome 21 segment HS21 C002 4210 9203 14185 4. 16 2. 0E-83 AF202879. 1 NT Homo sapiens hematopoZeUc progenitor cell antigen CD34 precursor (CD34) mRNA, partial cds 4517 9507 14487 6. 32 2. 0E-83 7706398 NT Homo sapiens ankyrin repeat-containing protein ASB-2 (LOC51676), mRNA 4517 9507 14488 6. 32 2. 0E-83 7706398 NT Homo sapiens ankyrin repeat-containing protein ASB-2 (LOC51676), mRNA Homo sapiens hdyroxyacyl-Coenzyme A dehdyrogenease/3-ketoacyl-Coenzyme A thiolase/enoyl-Conezyme A 1387 6384 11435 2.31 1.0E-83 4504326 NT hydratase (trifunctional protein), beta subunit (HADHB) mRNA Homo sapiens hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacy !-Coenzyme A thiolase/enoyf-Coenzyme A 1387 6384 11436 2. 31 1. 0E-83 4504326 NT hydratase (trifunctonal protein), beta subunit (HADHB) mRNA 2585 7548 12663 1. 22 1. 0E-83 BE883690. 1 EST-HUMAN 601507375F1 NIH_MGC_71 Homo sapiens cDNA clone IMAGE : 3908754 5' 3777 8780 13783 5. 31 1. 0E-83 AF053768. 1 NT Rattus norvegicus brain specific cortactin-binding protein CBP90 mRNA, partial cds 4122 9116 14103 3. 74 1. OE-83 Z25822. 1 NT H. sapiens gene for mitochondrial dodecenoyi-CoA delta-isomerase, exon 3 4733 9718 14703 2. 36 1. 0E-83 4502166 NT Homo sapiens amyfoid beta (A4) precursor protein (protease nexini-ll, Alzheimer disease) (APP), mRNA 3707 8711 13714 4. 14 7. 0E-84 BE901209. 1 EST_HUMAN 601676023F1 NIH_MGC_21 Homo sapiens cDNA clone IMAGE : 3958853 5' 1276 6274 11312 2. 87 6. 0E-84 BE838864. 1 ESTHUMAN RC2-FN0119-200600-011-g05 FN0119 Homo sapiens cDNA 1276 6274 11314 2. 87 6. 0E-84 BE838864. 1 EST HUMAN RC2-FN0119-200600-011-g05 FN0119 Homo sapiens cDNA 2334 7308 12429 3. 11 6. 0E-84 AA776574. 1 EST-HUMAN ae86a03.s1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE:971020 3' 5044 10015 14984 0. 98 6. 0E-84 R05859. 1 EST_HUMAN ye93aO4. r1 Soares fetal liver spieen 1 NFLS Homo sapiens cDNA clone IMAGE : 125262 5' 704 5728 10745 0.8 5. 0E-84 AA382811. 1 EST_HUMAN EST96094 Testis Homo sapiens cDNA 5' end 2945 7964 1.24 5.0E-84 AF109718.1 NT Homo sapiens chromosome 3 subtelomeric region wa76c04. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 2302086 3'similar to 1386 6383 11434 2. 71 4. OE-84 685321. 1 EST HUMAN SW : NRDC HUMAN 043847 NARDILYSIN PRECURSOR ; 4792 9776 14760 1. 93 4. 0E-84 450592E NT Homo sapiens polymerase (DNA-directed), alpha (70kD0 (POLA2), mRNA 4793 9777 14761 1.73 4.0E-84 AF069601.2 NT Homo sapiens myosin light chain kinase isoform 2 (MLCK) mRNA, complate cds 314 5369 10380 1. 77 3. OE-84 AF026200. 1 NT Homo sapiens Bach1 protein homolog mRNA, partial cds 1920 6906 12000 1. 89 3. 0E-84 5453855 NT Homo sapiens pericentriolar material 1 (PCM1) mRNA 1958 6944 12046 3. 98 3. 0E-84 AL096880. 1 NT Novel human mRNA containing Zinc finger C2H2 type domains Homo sapiens DNA, DLEC1 to ORCTL4 gene region, section 1/2 (DLEC1, ORCTL3, ORCTL4 genes, 3510 8518 13529 0. 97 3. 0E-84 AB026898. 1 NT complete cds) Page 142 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe exon @@@@ @@@@@ Top Hit OR SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. Value 3662 8667 13673 5. 61 3.0E-84 AF014459. 1 NT Homo sapiens X-linked juvenile retinoschisis precursor protein (XLRS1) mRNA, complete cds 2047 7029 12141 4.68 2.0E-84 BE695397. 1 EST HUMAN CM1-BT0795-190600-272-b08 BT0795 Homo sapiens cDNA 2047 7029 12142 4. 68 2. OE-84 BE695397. 1 EST HUMAN CM1-BT0795-190600-272-b08 BT0795 Homo sapiens cDNA 2873 7892 12915 8. 93 2. OE-84 AF036943. 1 NT Homo sapiens myelin transcription factor 1-like (MYT1-l) mRNA, complete cds 2892 7911 12932 1. 41 2. OE-84 X89211. 1 NT H. sapiens DNA for endogenous retroviral like element 310 5365 10376 1.21 1.A84 AF114488, 1 NT Homo sapiens intersect short isoform (ITSN) mRNA, complete cds Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide 545 5579 10583 16. 96 1. OE-84 4507952 NT (YWHAZ) mRNA 710 5734 1. 23 1. OE-84 11427631 NT Homo sapiens complement component 5 (C5), mRNA 1274 6272 11311 2. 76 1. OE-84 AA984379. 1 EST_HUMAN am85b11.s1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE : 1629885 3' 2001 6984 12088 1. 81. 0E-84 BE392137. 1 EST_HUMAN 601308006F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3626257 5' 2162 7141 12258 1.8 1.0E-84 11427197 NT Homo sapiens pericentriolar material 1 (PCM1), mRNA 3665 8670 13675 2.55 1.0E-84 AA720851. 1 EST_HUMAN nw12e06.s1 NCI_CGAP_SS1 Homo sapiens cDNA clone IMAGE : 1239106 3' 4292 9284 14271 4. 33 1. OE-84 AJ229041. 1 NT Homo sapiens 959 kb contig between AML1 and CBR1 on chromosome 21q22 ; segment 1/3 4561 9549 14534 2. 93 1.0E-84 AL043314. 2 EST_HUMAN DKFZp434N0323_R1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434N0323 5' 4561 9549 14535 2. 93 1. OE-84 AL043314. 2 EST_HUMAN DKFZp434N0323 r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434N0323 5' 4756 9284 14271 2. 67 1. OE-84 AJ229041. 1 NT Homo sapiens 959 kb contig between AML1 and CBR1 on chromosome 21q22 ; segment 1/3 4952 9929 14907 0.71 1.0E-84 AW371947. 1 EST HUMAN RC4-BT0311-141299-012-g06 BT0311 Homo sapiens cDNA 4952 9929 14908 0. 71 1.0E-84 AW371947. 1 EST HUMAN RC4-BT0311-141299-012-g06 BT0311 Homo sapiens cDNA 952 5968 2. 75 9. OE-85 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21 C009 1056 6065 11094 10. 8 9. 0E-85 U51432. 1 NT Homo sapiens nuclear protein Skip mRNA, complete cds 1056 6065 11095 10. 8 9. OE-85 U51432. 1 NT Homo sapiens nuclear protein Skip mRNA, complete cds 1543 6541 11598 1. 1 9.0E-85 M33282. 1 NT Human plasminogen gene, exon 7 1543 6541 11599 1. 1 9. OE-85 M33282. 1 NT Human plasminogen gene, exon 7 1635 6632 11702 2. 05 9. OE-85 7657020 NT Homo sapiens DKFZp434P211 protein (DKFZP434P211), mRNA 3711 8715 13717 0. 91 9. OE-85 7019418 NT Homo sapiens nucleolar GTPase (HUMAUANTIG), mRNA 4130 9125 14109 1. 08 9.0E-85 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21 C080 4639 9624 14615 1. 44 9. OE-85 M33764. 1 NT Human ornithinedecarboxy) asegene, compietecds 4639 9624 14616 1. 44 9. OE-85 M33764. 1 NT Human ornithine decarboxyiase gene, complete cds 4757 9741 14726 1.1 9.0E-85 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21 C068 1119 6125 11155 34. 29 B7.0E-85 L05094. 1 NT Homo sapiens ribosomal protein L27 mRNA, complete cds 2271 7247 12364 2. 11 5.0E-85 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 1280 6279 11320 0.77 3.0E-85 AF096157. 1 NT Homo sapiens protein phosphatase 2A BR gamma subunit gene, exon 6 1742 6737 11813 6.32 3. 0E-85 T97495. 1 EST HUMAN ye53g09. r1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 121504 5' Page 143 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession pabase ID NO : Signal BLAST E No. Top Hit Descriptor NO : NO : Value Source 4188 9181 14164 1. 26 3. OE-85 BE267189. 1 EST HUMAN 601189704F2 NIH MGC 7 Homo sapiens cDNA clone IMAGE : 3533616 5' 4743 9728 14714 1. 48 3. OE-85 11024695 NT Homo sapiens F-box only protein 24 (FBX024), mRNA 4743 9728 14715 1. 48 3. OE-85 11024695 NT Homo sapiens F-box only protein 24 (FBX024), mRNA 4809 9793 14775 1 3. OE-85 7363442 NT Homo sapiens olfactory receptor, family 12, subfamily D, member 2 (OR12D2), mRNA 948 5964 10998 0. 72 2. OE-85 7657266 NT Homo sapiens KIAA0929 protein Msx1 interacting nuclear target (MINT) homolog (KIAA0929), mRNA 1023 6033 11064 1. 96 2. OE-85 AF248540. 1 NT Homo sapiens intersec6n 2 (SH3D1 B) mRNA, complete cds 1380 6377 11425 1. 33 2. OE-85 7706205 NT Homo sapiens CGI-201 protein (LOC51340), mRNA 1395 6392 11445 7. 52 2. OE-85 5174775 NT Homo sapiens apolipoprotein C-II (APOC2) mRNA 1395 6392 11446 7. 52 2. OE-85 5174775 NT Homo sapiens apolipoprotein C-II (APOC2) mRNA 2169 7148 12265 1. 4 2. OE-85 U10525. 1 NT Human DNA polymerase beta gene, exons 12 and 13 2750 6315 10. 24 2. OE-85 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM121 (POM121 L1), mRNA 2956 7975 12990 2. 18 2. OE-85 M30938. 1 NT Human Ku (p70/p80) subunit mRNA, complete cds 4207 9200 14182 5. 76 2. OE-85 4505880 NT Homo sapiens plasminogen (PLG) mRNA 4755 9740 14725 0. 97 2. OE-85 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 2223 7200 3. 19 1. OE-85 BE794306. 1 EST HUMAN 601591416F1 NIH_MGC 7 Homo sapiens cDNA clone IMAGE : 3945818 5' 2329 7303 12423 5. 67 1. OE-85 BE618392. 1 EST_HUMAN 601462817F1 NIH MGC 67 Homo sapiens cDNA clone IMAGE : 3866021 5' 2329 7303 12424 5. 67 1. OE-85 BE618392. 1 EST HUMAN 601462817F1 NIH MGC 67 Homo sapiens cDNA clone IMAGE : 3866021 5' 1404 6401 54. 78 9. OE-86 BE274217. 1 EST HUMAN 601120778F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 2967690 5'I 923 5940 10972 0. 75 7. OE-86 AA860801. 1 EST HUMAN aj88f08. s1 Soaresarathyroid tumw_NbHPA Homo sapiens cDNA clone IMAGE : 1403559 3' 923 5940 10973 0. 75 7. OE-86 AA860801. 1 EST HUMAN aj88f08. s1 Soaresarathyroid tuma NbHPA Homo sapiens cDNA clone IMAGE : 1403559 3' 1275 6273 11312 2. 27 6. OE-86 4505492 NT Homo sapiens oxoglutarate dehydrogenase (lipoamida) (OGDH) mRNA 5001 9972 14948 1. 8 6. OE-86 6005833 NT Homo sapiens 24 kDa intrinsic membrane protein (PMP24), mRNA 211 5274 10288 1. 35 4. OE-86 BE547173. 1 EST HUMAN 601072594F1 NIH MGC 12 Homo sapiens cDNA clone IMAGE : 3458830 5' 264 5323 10332 2. 04 2. OE-86 AA306264. 1 EST HUMAN EST177232 Jurkat T-cells VI Homo sapiens cDNA 5'end 411 5448 2. 83 2. OE-86 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 1170 6173 11207 1. 94 2. OE-86 N58977. 1 EST HUMAN Y98. r1 Soares multiple sclerosis 2NbHMSP Homo sapiens cDNA clone IMAGE : 283478 5' 2128 7108 12222 3. 37 2. OE-86 9635487 NT Human endogenous retrovirus, complete genome 2207 7184 12307 1. 08 2. OE-86 AB033103. 1 NT Homo sapiens mRNA for KIAA1277 protein, part'al cds 3331 8341 13359 1. 19 2. OE-86 AW966142. 1 EST HUMAN EST378215 MAGE resequences, MAGI Homo sapiens cDNA 3661 8666 13671 2. 64 2. OE-86 AF156776. 1 NT Homo sapiens lysophosphatidic acid acyltransferase-delta (LPAAT-delta) mRNA, complete cds 3661 8666 13672 2. 64 2. OE-86 AF156776. 1 NT Hano sapiens lysophospha6dic acid acylUansferase-delta (LPAAT-delta) mRNA, complete cds gg2 2. 69 2. OE-86 AW 515742. 1 EST HUMAN hd87g08. x1 NCI_CGAP GC6 Homo sapiens cDNA clone IMAGE : 2916542 3' 4645 9630 14625 3. 42 2. OE-86 AF056490. 1 NT Homo sapiens cAMP-specific phosphodiesterase 8A (PDE8A) mRNA, partial cds Page 144 of 209 Table 4 Single Exon probes Expressed in HRI 100 C ploc Most similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databse Top Hit Descriptor ID NO: Signal RI ASTE No ID NO : Signas BLAST E No. source NO: NO: Source Value Homo sapiens NADH dehydrogenase (ubiquinone) Fe-S protein 1 (75kD) (NADH-coenzyme Q reductase) 1562 6559 11621 2.08 1.0E-86 4826855 NT (NDUFS1) mRNA 3089 8105 13120 1. 45 1. OE-86 5453649 NT Homo sapiens fibulin 5 (FBLN5) mRNA 3164 8180 13202 2.68 1.0E-86 L20492.1 NT Human gamma-glutamyl transpeptidase mRNA, complete cds 3222 8237 13258 1.17 1.0E-86 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21C009 3222 8237 13259 1.17 1.0E-86 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21 C009 3842 8844 13852 1. 22 1.0E-86 7706161 NT Homo sapiens hypothetical protein (LOC51318), mRNA 3842 8844 13853 1. 22 1. OE-86 7706161 NT Homo sapiens hypothetical protein (LOC51318), mRNA 4142 9137 14121 5. 37 1. OE-86 AL163300. 2 NT Homo sapiens chromosome 21 segment HS21C100 476 5512 10525 131. 14 8. OE-87 X62245. 1 NT O. cuniculus mRNA for elongation factor 1 alpha 2233 7210 12325 1.99 7.0E-87 BF063211. 1 EST_HUMAN 7h85f02.x1 NCI_GCAP_Co16 Homo sapiens cDNA clone IMAGE : 3322779 3' 2233 7210 12326 1. 99 7. OE-87 BF063211. 1 EST_HUMAN 7h85f02.x1 NCI_CGAP_Co16 Homo sapiens cDNA clone IMAGE : 3322779 3' 3448 8456 13482 0. 78 6. OE-87 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 1140 6145 11176 2. 38 5. OE-87 AA382811. 1 EST HUMAN EST96094 Testis I Homo sapiens cDNA 5'end 951 5967 11000 1.12 4.0E-87 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 1153 6157 11190 18. 02 4.0E-87 AB037835. 1 NT Homo sapiens mRNA for KIAA1414 protein, partial cds 1980 6965 12071 1. 85 4. OE-87 AB007925. 1 NT Homo sapiens mRNA for KIAA0456 protein, partial cds 2355 7329 12444 1. 2 4. OE-87 7706299 NT Homo sapiens CGI-60 protein (LOC51626), mRNA 2355 7329 12445 1. 2 4. OE-87 7706299 NT Homo sapiens CGI-60 protein (LOC51626), mRNA Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog) ; translocated to, 4 3383 8391 13414 1. 88 4. OE-87 5174574 NT (MLLT4) mRNA 2704 7661 12773 5. 54 2. OE-87 4885420 NT Homo sapiens high-mobility group (nonhistone chromosomal) protein 4 (HMG4) mRNA 2878 7897 1. 17 2. OE-87 BF327920. 1 ESTHUMAN QVO-BN0148-050600-254-a03 BN0148 Homo sapiens cDNA 3696 8700 13703 0. 88 2. OE-87 AU116935. 1 EST_HUMAN AU116935 HMBA1 Homo sapiens cDNA clone HEMBA1000307 5' 4758 9742 14727 0.66 2.0E-87 BF376311. 1 EST HUMAN CMO-TN0038-150900-552-h08 TN0038 Homo sapiens cDNA 1163 7698 1. 89 1. OE-87 7705683 NT Homo sapiens putative glycolipid transfer protein (LOC51054), mRNA 1406 6403 11460 1. 69 1. OE-87 AW361977. 1 ESTHUMAN PM2-CT0265-141099-001-g04 CT0265 Homo sapiens cDNA 1406 6403 11461 1. 69 1.0E-87 AW361977. 1 EST HUMAN PM2-CT0265-141099-001-g04 CT0265 Homo sapiens cDNA 3628 8635 13640 13. 271. 0E-87 Y00052. 1 NT Human mRNA for T-cell cyclophilin 3649 8655 13661 2. 3 1. 0E-87 4758827 NT Homo sapiens neurexin III (NRXN3) mRNA 909 5926 10961 0.7 9.0E-88 5453887 NT Homo sapiens protease inhibitor 4 (kallistatin) (Pl4) mRNA 1089 6096 11125 6.3 9.0E-88 AF167465.1 NT Homo sapiens double stranded RNA activated protein kinase (PKR) gene, exon 12 1331 6329 11377 2. 48 9.0E-88 AB037820.1 NT Homo sapiens mRNA for KIAA1399 protein, partial cds 1331 6329 11378 2.48 9.0E-88 AB037820. 1 NT Homo sapiens mRNA for KIAA1399 protein, partial cds Page 145 of 209 Table 4 Since Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit ID NO: Signal BLAST E No. Database Top Hit Descriptor Value Value 3542 8549 13557 0. 89 9. OE-88 AL163209. 2 NT Homo sapiens chromosome 21 segment HS21 C009 4147 9142 14126 3.04 9.0E-88 X91929. 1 NT H. sapiens ECE-1 gene (exon 9) 4147 9142 14127 3.04 9.0E-88 X91929. 1 NT H. sapiens ECE-1 gene (exon 9) 1792 6783 1. 98 5. OE-88 7661887 NT Homo sapiens KIAA0063 gene product (KIAA0063), mRNA K9719F Human fetal heart, Lambda ZAP Express Homo sapiens cDNA clone K9719 5'similar to ZINC 2568 7531 12650 4. 62 5. 0E-88 N89399. 1 EST HUMAN FINGER PROTEIN HZF1 2931 7950 12967 0.71 5.0E-88 AF114488. 1 NT Homo sapiens intersectin short isoform (ITSN) mRNA, complete cds 2941 7960 12979 0. 76 5. OE-88 AF114488. 1 NT Homo sapiens intersect short isoform (ITSN) mRNA, compelte cds 2941 7960 12980 0.76 5.0E-88 AF114488. 1 NT homo sapiens intersectin short isoform (ITSN) mRNA, complete cds wd68h08.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 2336799 3'similar to contains Alu 3305 8316 2. 56 5.0E-88 AI693217. 1 EST HUMAN repetitive element ; contains element MER22 MER22 repetitive element ; 3458 8466 13493 0.7 5.0E-88 AF114488. 1 NT Homo sapiens intersect short isoform (ITSN) mRNA, complete cds 1309 6307 11355 1.13 4.0E-88 BF091229. 1 EST_HUMAN PM1-TN0028-050900-004-f10 TN0028 Homo sapines cDNA 1309 6307 11356 1. 13 4. OE-88 BF091229. 1 EST HUMAN PM1-TN0028-050900-004-f10 TN0028 Homo sapiens cDNA 722 5745 10764 1. 4 3. OE-88 11545800 NT Homo sapiens hypothetical protein FLJ21634 (FLJ21634), mRNA 1776 6768 2. 26 3. OE-88 4508020 NT Homo sapiens zinc finger protein 259 (ZNF259) mRNA 2877 7896 12920 4. 85 3. OE-88 N66951. 1 EST HUMAN za48f12. s1 Soares fetal liver spleen 1NFLS Hono sapiens cDNA clone IMAGE : 295823 3' 4118 9112 14096 0. 69 3.0E-88 4501912 NT Homo sapiens a disintegrin and metalloproteinase domain 23 (ADAM23) mRNA 4118 9112 14097 0.69 3.0E-88 4501912 NT Homo sapiens a disintegrin and metalloproteinase domain 23 (ADAM23) mRNA 4351 9342 3.64 3.0E-88 11429300 NT Homo sapiens hypothetical protein FL J20220 (FLJ20220), mRNA 1019 6029 11058 1. 27 2. OE-88 7305198 NT Homo sapeins Calsenilin, presenilin-binding protein, EF hand transcription factor (CSEN), mRNA 1585 6582 11644 1.92 2.0E-88 AF246219. 1 NT Homo sapiens SNARE protein kianse SKAK mRNA, complete cds 1711 6706 11782 4. 8 2. OE-88 AF246219. 1 NT Homo sapiens SNARE protein kinase SNAK mRNA, complete cds 3384 8392 13415 0. 99 2. OE-88 AF246219. 1 NT Homo sapiens SNARE protein kinase SNAK mRNA, complete cds 4301 9293 14280 2. 35 2. OE-88 5031666 NT Homo sapiens dynein, axonemal, light polypeptide 4 (DNAL4), mRNA 2661 7620 12732 1. 49 8. OE-89 BE311557. 1 EST_HUMAN 601142409F1 NIH_MGC_14 Homo sapiens cDNA clone IMAGE : 3506186 5' 430 5468 10486 1. 24 7. OE-89 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 430 5468 10487 1. 24 7. OE-89 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 4730 9715 14700 3. 49 7.a89 4557390 NT Homo sapiens complement component 8, beta polypeptide (C8B) mRNA 4774 9758 14746 5. 7 7.0E-89 AL045748. 1 EST HUMAN DKFZp434E246 r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434E246 5' 1007 6017 11047 2.12 6.0E-89 5803114 NT Homo sapiens inner membrane protein, mitochondrial (mitofilin) (IMMT), mRNA 2151 7130 12247 1. 98 6.0E-89 4506124 NT Homo sapiens serine/threonine-protein kinase PRP4 homolog (PRP4) mRNA 2366 7340 12456 5l05 6.0E-89 4507788 NT Homo sapiens ubiquitin-conjugating enzyme E2L 3 (UBE2L3) mRNA 2366 7340 12457 5. 05 6. 0E-89 4507788 NT Homo sapiens ubiquitin-conjugating enzyme E2L 3 (UBE2L3) mRNA Page 146 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databas@ ID NO : Signal BLAST E No. Source NO: NO: Source Value 3446 8454 13480 0.75 6.0E-89 7661817 NT Homo sapiens HSPC159 protein (HSPC159), mRNA 4354 9345 14324 0. 91 6. OE-89 7661737 NT Homo sapiens HSPC019 protein (HSPC019), mRNA 4503 9493 14470 3. 9 6. 0E-89 AB007866. 2 NT Homo sapiens mRNA for KIAA0406 protein, partial cds 4503 9493 14471 3. 9 6. 0E-89 AB007866. 2 NT Homo sapiens mRNA for KIAA0406 protein, partial cds TCBAP2E0383 Pediatric pre-B cell acute iymphobjastic leukemia Baylor-HGSC project=TCBA Homo sapiens 4903 9882 14852 3. 31 5. OE-89 BE244323. 1 EST_HUMAN cDNA clone TCBAP0383 TCBAP2E0383 Pediatric pre-B cell acute lymphoblastic leukemia Baylor-HGSC project=TCBA Homo sapiens 4903 9882 1485 3. 31 5. OE-89 BE244323. 1 EST HUMAN cDNA clone TCBAP0383 2807 7827 12843 0. 95 3. OE-89 AW976181. 1 EST HUMAN EST388290 MAGE resequences, MAGN Homo sapiens cDNA 127 5442 10462 0. 87 2. 0E-89 7706670 NT Homo sapiens PXR2b protein (PXR2b), mRNA 127 5442 10463 0. 87 2. 0E-89 7706670 NT Homo sapiens PXR2b protein (PXR2b), mRNA 526 5561 10564 0. 68 2. 0E-89 AB037763. 1 NT Homo sapiens mRNA for KIAA1342 protein, partial cds qg96c08. x1 Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE : 1843022 3'similar to gb : J04131 2811 7831 12847 1. 44 2. 0E-89 Al222095. 1 EST_HUMAN GAMMA-GLUTAMYLTRANSPEPTDASE 1 PRECURSOR (HUMAN) ; contains Alu repetitive element, 4022 9018 14005 1.18 2. 0E-89 AF089897. 1 NT Homo sapiens topoisomerase-related function protein (TRF4-2) mRNA, partial cds 4030 9026 14014 4. 76 2. OE-89 X58742. 1 NT H. sapiens HCK gene for tyrosine kinase (PTK), exons 10-11 4030, 9026 14015 4. 76 2. 0E-89 X58742. 1 NT H. sapiens HCK gene for tyrosine kinase (PTK), exons 10-11 4223 9217 14196 1. 09 2.0E-89 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 4367 9359 14339 1. 05 2. 0E-89 AJ007378. 1 NT Homo sapiens GGT gene, exon 5 1046 6056 11084 3. 19 8. OE-90 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 1047 6056 11084 2. 72 8.0E-90 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 1310 7744 11357 3. 66 8. 0E-90 BE670561. 1 EST_HUMAN 7e36f08.X1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3284583 3' 1310 7744 11358 3. 66 8. 0E-90 BE670561. 1 EST-HUMAN 7e36f08.x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3284583 3' Homo sapiens calcium channel alpha subunit (CACNA1E) gene, exons 7-49, and partial cds, aiternativefy 826 5846 4. 22 7. 0E-90 AF223391. 1 NT spliced 2994 8012 13024 1. 08 6. 0E-90 X91926. 1 NT H. sapiens ECE-1 gene (exon 6) 2994 8012 130251. 08 6. 0E-90 X91926. 1 NT H. sapiens ECE-1 gene (exon 6) 4105 9099 14085 9. 58 6. 0E-90 8922398 NT Homo sapiens hypothetical protein FLJ10388 (FLJ10388), mRNA 4105 9099 14086 9. 58 6. OE-90 8922398 NT Homo sapiens t 154 5220 78.69 5.0E-90 AB035344. 1 NT Homo sapiens TCL6 gene, exon 1-10b 1173 6176 11210 2. 39 5.0E-90 U80226. 1 NT Human gamma-aminobutyric acid transaminase mRNA, partial cds Page 147 of 209 Table 4 Single Exop probes Expressed in HRI 100 Cells Probe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source NO: NO: Source Value qg96c08.x1 Soeres_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE:1843022 3 similar to gb:J04131 1784 6776 11867 1. 48 5. OE-90 AI222095. 1 EST HUMAN GAMMA-GLUTAMYLTRANSPEPTIDASE 1 PRECURSOR (HUMAN) ; contains Alu repetitive element ; qg96c08. x1 Soares NFL T GBC S1 Homo sapiens cDNA clone IMAGE : 1843022 3'similar to gb : J04131 1784 6776 11868 1.48 5.0E-90 AI222095. 1 EST HUMAN GAMMA-GLUTAMYLTRANSPEPTIDASE 1 PRECURSOR (HUMAN) ; contains Alu repetitive element ; 2484 7452 12566 1. 82 5. 0E-90 AF114487. 1 NT Homo sapiens intersectin long isoform (ITSN) mRNA, complete cds 300 5357 10369 2.4 4.0E-90 AF231920. 1 NT Homo sapiens chromosome 21 unknown mRNA 300 5357 10370 2. 4 4. OE-90 AF231920. 1 NT Homo sapiens chromosome 21 unknown mRNA 1070 6078 11109 3. 28 4.0E-90 4505316 NT Homo sapiens myosin phosphatase, target subunit 1 (MYPT1), mRNA 1650 6646 11719 9. 18 4. OE-90 X99033. 1 NT H. sapiens gene encoding discoidin receptor tyrosine kinase, exon 16 4522 9512 14497 4. 85 4. OE-90 D87675. 1 NT Homo sapiens DNA for amyloid precursor protein, complete cds 4653 9638 14629 1. 95 4. OE-90 AB033070. 1 NT Homo sapiens mRNA for KIAA1244 protein, partial cds 4673 9658 14641 1. 82 4. OE-90 M95967. 1 NT Human prohormone converting enzyme (NEC2) gene, exon 8 5010 9981 14956 0. 92 4. OE-90 5729777 NT Homo sapiens colalgen, type Xil, alpha 1 (COL12A1), mRNA 213 5276 10290 4. 2 2. OE-90 BE537913. 1 EST_HUMAN 601067378F1 NIH_MGC_10 Homo sapiens cDNA clone IMAGE : 3453834 5' 1154 6158 11191 71. 49 2. OE-90 5031748 NT Homo sapiens high-mobility group (nonhistone chromosomal) protein 17 (HMG17), MRNA 1154 6158 11192 71. 49 2. OE-90 5031748 NT Homo sapiens high-mobility group (nonhistone chromosomal) protein 17 (HMG17), mRNA qc54c02. x1 Soaresptacenta8to9weeks2NbHP8to9W Homo sapiens cDNA clone IMAGE : 1713410 3' 3755 8758 13757 1.88 2.0E-90 AI138213.1 EST-HUMAN similar to SW : OLF3 MOUSE P23275 OLFACTORY RECEPTOR OR3. ; 4550 9538 14524 0.97 2.0E-90 AB006627. 1 NT Homo sapiens mRNA for KIAA0289 gene, partial cds 4754 9739 14724 8. 45 2. OE-90 5729855 NT Homo sapiens GRB2-related adaptor protein (GRAP) mRNA 274 5333 10346 5.3 1.0E-90 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) (APP), mRNA 373 7693 10436 1. 98 1.0E-90 AF231920. 1 NT Homo sapiens chromosome 21 unknown mRNA 374 7693 10436 1. 9 1. OE-90 AF231920. 1 NT Homo sapiens chromosome 21 unknown mRNA 686 5710 10723 1. 73 1. OE-90 AJ237589. 1 NT Homo sapiens mRNA for T-box transcription factor (TBX20 gene), partial 686 5710 10724 1. 73 1.0E-90 AJ237589. 1 NT Homo sapiens mRNA for T-box transcription factor (TBX20 gene), partial 720 5743 10761 11. 11 1. 0E-90 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds 720 5743 10762 11. 11 1.0E-90 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds 1093 6100 3 1. OE-90 4507828 NT Homo sapiens Kruppel-like factor 7 (ubiquitous) (KLF7), mRNA 1287 6286 11329 2. 29 1.0E-90 AF096154. 1 NT Homo sapiens protein phosphatase 2A BR gamma subunit gene, exon 3 1287 6286 11330 2.29 1.0E-90 AF096154.1 NT Homo sapiens protein phosphatase 2A BR gamma subunit gene, exon 3 1628 6625 4.57 1.0E-90 BE379884. 1 EST HUMAN 601159563F2 NIH MGC_53 Homo sapiens cDNA clone IMAGE : 3511118 5' Page 148 of 209 Table 4 Single Exon Probes Expessed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession patabase ID NO : Signal BLAST E No. Top Hit Descriptor Source. NO : NO : Value 1861 6850 11938 2 : 56 1. OE-90 11420514 NT Homo sapiens similar to SALL1 (sal (Drosophila)-like (LOC57167), mRNA 2780 7801 12819 9. 3 1. OE-9D 6005720 NT Homo sapiens chromosome 8 open reading frame 2 (C80RF2), mRNA 3761 8764 13765 0. 99 1. OE-90 AB020710. 1 NT Homo sapiens mRNA for KIAA0903 protein, partial cds 3761 8764 13766 0. 99 1. OE-90 AB020710. 1 NT Homo sapiens mRNA for KIAA0903 protein, partial cds Homo sapiens soluble interleukin 1 receptor accessory protein (IL1RAP) gene, exon S, alternative exons 9 42gg g291 14278 1. 62 1. OE-90 AF167340. I. NT and complete cds, alterna6vely spliced 4073 9067 14057 6. 67 8. OE-91 D12234. 1 EST HUMAN HUMOOOS381 Liver HepG2 cell line. Homo sapiens cDNA clone s381 3' 3394 8402 13428 2. 26 5. OE-91 AA702794. 1 EST HUMAN zi9b04. s1 Soares fetal liver spleen 1NFLS S1 Homo sapiens cDNA clone IMAGE : 448015 3' 4385 9376 14355 1. 19 5. OE-91 AU943539. 1 EST HUMAN AU143539 Y79AA1 Homo sapiens cDNA clone Y79AA1002087 5' 4385 9376 14356 1. 19 5. OE-91 AU143539. 1 EST HUMAN AU143539 Y79AA1 Homo sapiens cDNA clone Y79AA1002087 5' 4664 9649 14636 1. 09 5. OE-91 7110634 NT Homo sapiens chromosome 22 open reading frame 5 (C220RF5), mRNA 4664 9649 14637 1. 09 5. OE-91 7110634 NT Homo sapiens chromosome 22 open reading frame 5 (C220RF5), mRNA 3129 8145 13165 1. 67 4. OE-91 AF156776. 1 NT Homo sapiens lysophosphatidic acid acyltransferase-delta (LPAAT-delta) mRNA, complete cds 3129 8145 13166 1. 67 4. OE-91 AF156776. 1 NT Homo sapiens tysophospha6dic acid acyltransferase-delta (LPAAT-delta) mRNA, complete cds 1578 6575 11637 1. 86 3. OE-91 11430193 NT Homo sapiens solute carrier family 4, anion exchanger, member 3 (SLC4A3), mRNA 1578 6575 11638 1. 86 3. OE-91 11430193 NT Homo sapiens solute carrier family 4, anion exchanger, member 3 (SLC4A3), mRNA 1754 7701 11830 1. 4 3. OE-91 AF265555. 1 NT Homo sapiens ubiquitin-conjugating BIR-domain enzyme APOLLON mRNA, complete cds 3265 8278 9 3301 1. 55 3. OE-91 AL163283. 2 NT Homo sapiens chromosome 21 segment HS21 C083 3380 8388 13410 3. 62 3. OE-91 AB033104. 1 NT Homo sapiens mRNA for KIAA1278 protein, partial cds 3380 8388 13411 3. 62 3. OE-91 AB033104. 1 NT Homo sapiens mRNA for KIAA1278 protein, partial cds _ 3699 8703 13706 1. 57 3. OE-91 AF084530. 1 NT Homo sapiens cyclin-D binding Myb-like protein mRNA, complete cds 4458 9448 14428 6. 17 3. OE-91 M30938. 1 NT Human Ku (p701p80) subunit mRNA, complete cds 4820 9804 14785 1. 2 3. OE-91 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 4820 9804 14786 1. 2 3. OE-91 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 49 5130 10124 2. 61 1. OE-91 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 1226 6225 11271 5. 11 1. OE-91 AW449746. 1 EST HUMAN UI-H-BI3-aks-d-01-0-UI. s1 NCI_CGAP Sub5 Homo sapiens cDNA clone IMAGE : 2735280 3' 1222 6222 11265 7. 33 9. OE-92 AJ001689. 1 NT Homo sapiens NKG2D gene, exon 10 9222 6222 11266 7. 33 9. OE-92 AJ001689. 1 NT Homo sapiens NKG2D gene, exon 10 91 5168 10178 6. 58 8. OE-92 W 26367. 1 EST HUMAN 26f3 Human retina cDNA randomly primed sublibrary Homo sapiens cDNA 283 5341 10354 7. 99 8. OE-92 BE386363. 1 EST HUMAN 601273513F1 NIH_MGC 20 Homo sapiens cDNA cione IMAGE : 3614667 5' 25 5105 10089 2. 65 7. OE-92 AB031007. 1 NT Homo sapiens DNA, MHC class I region, 7. 1 ancestral haplotype 235 7716 10307 1 7. OE-92 AB018301. 1 NT Homo sapiens mRNA for KIAA0758 protein, partial cds 235 7716 10308 1 7. OE-92 AB018301. 1 NT Homo sapiens mRNA fa KIAA0758 protein, partial cds 586 5617 0. 93 7. OE-92 AF007822. 1 NT Homo sapiens cytoplasmic Seprase truncated isofam mRNA, complete cds < Page 149 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acessioni @@@@@ ID NO: Signal BLAST E No. @@@@@@ @@@@@@@ NO: NO: Source Value 1261 6259 11302 2.83 7.0E-92 4502384 NT Homo sapiens B-cell CLL/lymphoma 7b (BCL7B) mRNA 2123 7103 12215 8. 39 7. 0E-92 5031570 NT Homo sapiens ARP2 (actin-related protein 2, yeast) homolog (ACTR2), mRNA 2123 7103 12216 8. 39 7. OE-92 5031570 NT Homo sapiens ARP2 (actin-related protein 2, yeast) homolog (ACTR2), mRNA 2493 7461 12576 2. 56 7 0E-92 AF167706 1 NT Homo sapiens cysteine-rich repeat-containing protein S52 precursor, mRNA, complete cds 2653 7613 12723 5. 83 7. OE-92 6005738 NT Homo sapiens NRAS-related gene (D1S155E), mRNA 2679 7637 12752 0. 93 7. 0E-92 AB031007. 1 NT Homo sapiens DNA, MHC class I region, 7. 1 ancestral haplotype 3270 10047 13304 0. 67 7. 0E-92 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 3270 10047 13305 0. 67 7. OE-92 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA N-CAM=145 kda neural cell adhesion molecule [human, small cell lung cancer cell line OS2-R, mRNA, 2960 4455 9445 144251. 24 7. 0E-92 S71824. 1 NT nt] N-CAM=145 kda neural cell adhesion molecule [human, small cell lung cancer cell line OS2-R, mRNA, 2960 4455 9445 14426 1. 24 7. 0E-92 S71824. 1 NT nt] 4844 9826 14801 0. 94 7. 0E-92 AL163281. 2 NT Homo sapiens chromosome 21 segment HS21 C081 1552 6549 1.37 5.0E-92 BE390882. 1 EST_HUMAN 601283012F1 NIH_MGC-44 Homo sapiens cDNA clone IMAGE : 3605018 5' 2692 7650 12764 2. 21 3. 0E-92 BE909714. 1 EST_HUMAN 601501242F1 NIH-MGC_70 Homo sapiens cDNA clone IMAGE : 3902939 5' 26 5106 10090 1. 42 2. 0E-92 4501898 NT Homo sapiens activin A receptor, type JIB (ACVR2B) mRNA 178 5241 10251 3. 37 2. 0E-92 11422946 NT Homo sapiens hypothetical protein dJ462023. 2 (DJ462023. 2), mRNA 178 5241 10252 3. 37 2. OE-92 11422946 NT Homo sapiens hypothetica) protein dJ462023. 2 (DJ462023. 2), mRNA 740 5763 10787 2. 34 2. 0E-92 BE299190. 1 EST HUMAN 601118337F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 3028304 5' 740 5763 10788 2. 34 2. 0E-92 BE299190. 1 EST_HUMAN 601118337F1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE : 3028304 5' 1676 6672 1. 45 2. OE-92 S78653. 1 NT mrg=mas-related [human, Genomic, 2416 nt] wk27d07. x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE : 2413549 3'similar to TR : Q12844 1896 6884 11976 1. 59 2. 0E-92 AI818119. 1 EST HUMAN Q12844 BREAKPOINT CLUSTER REGION PROTEIN ; wk27d07.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE : 2413549 3'similar to TR : Q12844 1896 6884 11977 1. 59 2. 0E-92 AI818119. 1 EST HUMAN Q12844 BREAKPOINT CLUSTER REGION PROTEIN ; 1995 6979 12084 8. 59 2. OE-92 4506860 NT Homo sapiens syndecan 4 (amphiglycan, ryudocan) (SDC4) mRNA 2588 7551 12666 15 93 2 0E-92 6912457 NT Homo sapiens calcineurin binding protein 1 (KIAA0330), mRNA 2756 6611 11676 3. 99 2. OE-92 11418424 NT Homo sapiens collagen, type XII, alpha 1 (COL12A1), mRNA 2756 6611 11677 3. 99 2. 0E-92 11418424 NT Homo sapiens collagen, type XII, alpha 1 (COL12A1), mRNA 3532 8538 13543 1. 16 2. 0E-92 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 3532 8538 13544 1. 16 2. 0E-92 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 3602 8609 13617 5. 87 2. 0E-92 5803180 NT Homo sapiens stress-induced-phosphoprotein1 (Hsp70/Hsp90-organizing protein) (STIP1), mRNA 4165 9160 14147 1. 16 2. 0E-92 M10976.1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment Page 150 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Most Similar SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acessin Top Hit ID NO : Signal BLAST E No. Source NO: NO: Source Value 4835 9819 2.79 2.0E-92 AL040437.1 EST-HUMAN DKFZp434C0414-r1 434 (synonym: htes3) Homo sapiens cDNA colone DKFZp434C0414 5' 1813 6803 11895 2.03 1.0E-92 R78078. 1 EST HUMAN yi80e08. r1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 145574 5' 1813 6803 11896 2.03 1.0E-92 R78078. 1 EST_HUMAN yi80e08.r1 Soares placenta Nb2HP Homo sapiens cDNA clone IMAGE : 145574 5' 2020 7003 12108 40.93 1.0E-92 4506668 NT Homo sapiens ribosomal protein, large, P1 (RPLP1) mRNA 1979 6964 12070 2.63 9.0E-93 AU121681. 1 EST-HUMAN AU121681 MAMMA1 Homo sapiens cDNA clone MAMMA1000738 5' EST188414 HCC cell line (matastasis to liver in mouse) II Homo sapiens cDNA 5'end similar to ribosomal 1991 6976 27.81 9.0E-93 AA316723. 1 EST HUMAN protein L29 3534 8540 13546 1.75 9.0E-93 BE388571. 1 EST-HUMAN 601281867F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3603832 5' 4209 9202 14184 1. 1 9.0E-93 AU121681.1 EST_HUMAN AU121681 MAMMA1 Homo sapiens cDNA clone MAMMA1000738 5' 244 5304 10314 8. 34 7. OE-93 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 1362 6359 11409 2. 07 5.0E-93 AB014511. 1 NT Homo sapiens mRNA for KIAA0611 protein, partial cds 1383 6380 11429 8. 53 5. OE-93 A1674184. 1 EST_HUMAN wc09c08.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE : 2314670 3' 1383 6380 11430 8.53 5.0E-93 AI674184. 1 EST_HUMAN wc09c08.x1 NCI_CGAP_Pr28 Homo sapiens cDNA clone IMAGE : 2314670 3' 3162 8178 13200 4.58 5.0E-93 X04201.1 NT Human skeletal muscle 1. 3 kb mRNA for tropomyosin ! zx50eO9. sl Soares-testis-NHT Homo sapiens cDNA clone IMAGE : 795688 3'similar to SW : CLPARAT 86 5163 5.69 4.0E-93 AA459933. 1 EST HUMAN P37397 CALPONIN, ACIDIC ISOFORM ; I 442 5479 10496 1. 62 4.0E-93 4557879 NT Homo sapiens interferon gamma receptor 1 (IFNGR1) mRNA 442 5479 10497 1. 62 4. OE-93 4557879 NT Homo sapiens interferon gamma receptor 1 (IFNGR1) mRNA 763 5784 10812 4.03 4.0E-93 7657454 NT Homo sapiens pescadillo (zebrafish) homolog 1, containing BRCT domain (PES1), mRNA 763 5784 10813 4. 03 4.0E-93 7657454 NT Homo sapiens pescadillo (zebrafish) homolog 1, containing BRCT domain (PES1), mRNA 1164 6167 11201 1. 25 4. OE-93 8923658 NT Homo sapiens hypothetical protein FLJ20731 (FLJ20731), mRNA 1932 6918 12017 3. 59 4. OE-93 AF047677. 1 NT Homo sapiens dystrophin (DMD) gene, deletion breakpoints 1-3 in intron 5 2183 7162 12282 0.93 4.0E-93 AF157476. 1 NT Homo sapiens DNA polymerase zeta catalytic subunit (REV3) mRNA, complete cds 2533 7498 12618 1. 01 4. OE-93 7656972 NT Homo sapiens TNF-inducibie protein CG12-1 (CG12-1), mRNA 3487 8495 13512 0.79 4.0E-93 7705396 NT Homo sapiens tumor antigen SLP-8p (HCC8), mRNA 3935 8934 13927 5.14 4.0E-93 4504654 NT Homo sapiens interleukin 18 receptor 1 (IL18R1) mRNA 4863 8495 13512 0. 83 4. OE-93 7705396 NT Homo sapiens tumor antigen SLP-8p (HCC8), mRNA 3567 8574 13579 19.66 3. OE-93 BF690630. 1 EST_HUMAN 602246554F1 NIH_MGC_62 Homo sapiens cDNA clone IMAGE : 4332036 5' 3567 8574 13580 19. 66 3. OE-93 BF690630. 1 EST-HUMAN 602246554F1 NIH_MGC_62 Homo sapiens cDNA clone IMAGE : 4332036 5' 190 5254 10265 31. 68 2.0E-93 AB015610. 1 NT Chlorocebus aethiops mRNA for ribosomal protein S4X, complete cds 190 5254 10266 31. 68 2.0E-93 AB015610. 1 NT Chlorocebus aethiops mRNA for ribosomal protein S4X, complete cds 321 5376 10386 9. 39 2. OE-93 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21C085 322 5376 10386 6. 39 2. OE-93 AL163285. 2 NT Homo sapiens chromosome 21 segment HS21 C085 2072 7054 12164 1. 96 2. 0E-93 U40763. 1 NT Human Clk-associated RS cyclophilin CARS-Cyp mRNA, complete cds Page 151 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databse Top Hit Descriptor ID NO: Signal RI ASTE No NO : NO : ID NO : Signal BLAST E No. Source Value 2416 7387 12507 1.74 2.0E-93 BE252982.1 EST-HUMAN 601117586F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE:3358220 5' 4966 9943 14920 1. 01 2. 0E-93 BE253201. 1 EST_HUMAN 601116810F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE : 3357243 5' 102 5179 10189 2. 66 1. OE-93 AF238997. 1 NT Homo sapiens CTR1 pseudogene 102 5179 10190 2.66 1.0E-93 AF238997. 1 NT Homo sapiens CTR1 pseudogene 514 5549 10554 17. 48 1. OE-93 7657016 NT Homo sapiens hypothetical protein (DJ328E19. C1. 1), mRNA oy84b08. x1 NCI_CGAP_CLL1 Homo sapiens cDNA clone IMAGE : 1672503 3'similar to TR : Q62384 Q62384 595 5626 10625 3.67 1.0E-93 AI146755. 1 EST HUMAN ZINC FINGER PROTEIN. ; 861 5880 10921 7. 39 1. OE-93 D87675. 1 NT Homo sapiens DNA for amyioid precursor protein, complete cds 1217 6216 11256 8.15 1.0E-93 8923270 NT Homo sapiens hypothetical protein FLJ20291 (FLJ20291), mRNA 1217 6216 11257 8. 15 1. OE-93 8923270 NT Homo sapiens hypothetical protein FLJ20291 (FLJ20291), mRNA 1325 6323 11370 1.13 1.0E-93 AB046783. 1 NT Homo sapiens mRNA for KIAA1563 protein, partial cds 1327 6325 11372 3. 03 1 0E-93 AF167706 1 NT Homo sapiens cysteine-rich repeat-containing protein S52 precursor, mRNA, complete cds 2276 7252 12370 5. 14 1. OE-93 AF231981. 1 NT Homo sapiens long chain polyunsaturated fatty acid elongation enzyme (HEL01) mRNA, complete cds 2394 7365 12487 12. 91 1. OE-93 AF055066. 1 NT Homo sapiens MHC class 1 region 2435 7406 1.31 1.0E-93 AL137200. 1 NT Novel human gene mapping to chomosome 1 2749 6275 11315 1. 39 1. OE-93 BE297369. 1 EST_HUMAN 601177686F1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE : 3532965 5' 2749 6275 11316 1. 39 1.0E-93 BE297369. 1 EST_HUMAN 601177686F1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE : 3532965 5' 2863 7883 12903 3.34 1.0E-93 D87675.1 NT Homo sapiens DNA for amyloid precursor protein, complete cds 3144 8160 1.76 1.0E-93 AF231981. 1 NT Homo sapiens long chain polyunsaturated fatty acid elongation enzyme (HEL01) mRNA, complete cds 4305 9297 14283 2. 36 1. OE-93 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 3856 8858 13864 3. 44 6. OE-94 AF142482. 1 NT Homo sapiens transcription enhancer factor-5 mRNA, complete cds 1806 6797 38. 53 4. OE-94 L05094. 1 NT Homo sapiens ribosomal protein L27 mRNA, complete cds 2586 7549 12664 1. 13 4. OE-94 4506008 NT Homo sapiens protein phosphatase 1, regulatory subunit 10 (PPP1R10) mRNA 3587 8594 13598 1.02 4.A94 AW197851. 1 EST_HUMAN xn89f12.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2701679 3' 3587 8594 13599 1.02 4.0E-94 AW19785.1 EST-HUMAN xn89f12.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2701679 3' tw11f10. x1 NCl_CGAP_Brn52Homo sapiens cDNA clone IMAGE : 2259403 3'similar toTR : Q15265 Q15265 4579 9567 14556 3.55 4.0E--94 AI591312. 1 EST_HUMAN PROTEIN TYROSINE PHOSPHATASE ; 607 5634 10635 3. 13 3. OE-94 AB022785. 1 NT Homo sapiens ASH2L gene, complete cds, similar to Drosophila ash2 gene 711 5735 10751 1.52 3.0E-94 4502506 NT Homo sapiens complement component 5 (C5) mRNA 1701 6696 11772 3.56 3.0E-94 AF167706.1 NT Homo sapiens cysteine-rich repeat-containing protein S52 precursor, mRNA, complete cds 1701 6696 11773 3.56 3.0E-94 AF167706. 1 NT Homo sapiens cysteine-rich repeat-containing protein S52 precursor, mRNA, complete cds 1735 6730 11807 6.42 3.0E-94 4557556 NT Homo sapiens E1A binding protein p300 (EP300) mRNA Page 152 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells probe Exon Most Similar ORF SEQ Expressoin (Top) Hit Top Hit Acession top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Top hit Descriptor NO: NO: source Value 4065 9059 14046 0.67 3.0E-94 AA464805.1 EST_HUMAN zw63g08.r1 Soares_fotal_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE:774782 5' 5051 10022 14991 0. 7 3. 0E-94 4507848 NT Homo sapiens ubiquitin specific protease 13 (isopeptidase T-3) (USP13) mRNA 148 5214 10228 2. 88 1. OE-94 BE295714. 1 EST_HUMAN 601175762F1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE : 3531038 5' 3015 8032 13042 2. 59 1. 0E-94 BE253433. 1 EST HUMAN 601111696F1 NIH MGC 16 Homo sapiens cDNA clone IMAGE : 3352559 5' 3015 8032 13043 2. 59 1. OE-94 BE253433. 1 EST_HUMAN 601111696F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE : 3352559 5' 4233 9227 14211 1. 7 1. OE-94 9506692 NT Homo sapiens hypothetical protein (FLJ20746), mRNA 1447 6444 11503 5. 95 9. 0E-95 AF027302. 1 NT Homo sapiens TNF-aipha stimulated ABC protein (ABC50) mRNA, complete cds 3082 8098 13112 1. 09 9. OE-95 7662027 NT Homo sapiens KIAA0256 gene product (KIAA0255), mRNA 3082 8098 13113 1. 09 9. OE-95 7662027 NT Homo sapiens KIAA0255 gene product (KIAA0255), mRNA we09e04. x1 NCI_CGAP-Lu24 Homo sapiens cDNA clone IMAGE : 2340606 3'similar to gb : K00558 4406 9397 14380 3. 37 8. 0E-95 AI700998. 1 EST_HUMAN TUBULIN ALPHA-1 CHAIN (HUMAN) ; we09e04. x1 NCI_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 2340606 3'similar to gb : K00558 4406 9397 14381 3. 37 8. 0E-95 At700998. 1 EST HUMAN TUBULIN ALPHA-1 CHAIN (HUMAN) ; 273 5332 10344 10. 53 7. 0E-95 D87675. 1 NT Homo sapiens DNA for amyloid precursor protein, complete cds 273 5332 10345 10. 53 7. 0E-95 D87675. 1 NT Homo sapiens DNA for amyloid precursor protein, complete cds 4241 9235 14219 5. 66 7.0E-95 M95708. 1 NT Homo sapiens Ly-6-like protein (CD59) mRNA, complete cds 4289 9281 1. 4 7. 0E-95 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 1605 6601 11662 3. 13 2. OE-95 7662027 NT Homo sapiens KIAA0255 gene product (KIAA0255), mRNA 1605 6601 11663 3. 13 2. OE-95 7662027 NT Homo sapiens KIAA0255 gene product (KIAA0255), mRNA Homo sapiens tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy, pseudoinflammatory) (TIMP3) 1901 6888 11982 3. 11 2. 0E-95 4607512 NT mRNA 1904 6891 11986 1. 74 2. 0E-95 BE393873. 1 EST HUMAN 601312161 F1 NIH MGC 44 Homo sapiens cDNA clone IMAGE : 3658862 5' 2359 7333 12449 1. 55 2. OE-95 5453665 NT Homo sapiens G protein-coupled receptor 19 (GPR19) mRNA 2359 7333 12450 1. 55 2.0E-95 5453665 NT Homo sapiens G protein-coupled receptor 19 (GPR19) mRNA Homo sapiens glutathione S-transferase theta 2 (GSTT2) and glutathione S-transferase theta 1 (GSTT1) 2396 7367 12488 2. 79 2. 0E-95 AF240786. 1 NT genes, complete cds 2442 7412 12528 1. 84 2. 0E-95 4758423 NT Homo sapiens glycine cleavage system protein H (aminomethyl carrier) (GCSH) mRNA 3084 8100 13115 1. 95 2. 0E-95 AF015452. 1 NT Homo sapiens Usurpin-gamma mRNA, complete cds 3484 8492 13508 2. 78 2. 0E-95 7705900 NT Homo sapiens unconventional myosin-15 (LOC51168), mRNA 3484 8492 13509 2. 78 2. OE-95 7705900 NT Homo sapiens unconventional myosini-15 (LOC51168), mRNA 3537 8543 13549 1. 17 2. 0E-95 AB037807. 1 NT Homo sapiens mRNA for KIAA1386 protein, partial cds qm01c02. x1 SoaresNhHMPuS1 Homo sapiens cDNA clone IMAGE : 1880546 3'similar to WP : T23G7. 4 3664 8669 13674 1 2. OE-95 AI290264. 1 EST HUMAN CE03705 ; 4236 9230 14213 1. 61 2. 0E-95 7657185 NT Homo sapiens hypothetical protein (HS322B1A), mRNA Page 153 of 209 Table 4 Single Exon Probes Expressed in HBI 100 Cells Probe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Source NO: NO: Source Value 4783 9852 14827 2.65 2.0E-95 7661979 NT Homo sapiens KIAA0187 gene product (KIAA0187), mRNA 4917 9895 14869 0. 921 2.0E-95 AA447931. 1 EST_HUMAN zx11d07.r1 Soares_total_fetus_Nb2HF8_92 Homo saplens cDNA clone IMAGE:786157 5' 4917 9895 14870 0. 92 2.0E-95 AA447931. 1 EST_HUMAN zx11d07.r1 Soares_total-fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE : 786157 5' 439 7720 10493 3. 42 8. 0E-9 BE907607. 1 EST_HUMAN 601497608F1 NIH_MGC_70 Homo sapiens cDNA clone IMAGE : 3899761 5' 439 7720 10494 3. 42 8.0E-96 BE907607. 1 EST_HUMAN 601497608F1 NIH_MGC_70 Homo sapiens cDNA clone IMAGE : 3899761 5' 3813 8816 13822 1. 16 7.0E-96 AF231920.1 NT Homo sapiens chromosome 21 unknown mRNA 2198 7176 12299 2. 4 6. OE-96 BE171984. 1 EST HUMAN MRO-HT0559-250200-002-d07 HT0559 Homo sapiens cDNA 3244 8257 13278 0. 86 6.0E-96 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21C001 3402 8411 13437 37.31 6.0E-96 M26873.1 NT Human glyceraldehyde-3-phosphate dehydrogenase pseudogene 3'end 318 5373 10382 3.23 5.0E-96 AB032998. 1 NT Homo sapiens mRNA for KIAA1172 protein, partial cds 832 5851 10889 3.33 5.0E-96 AB032998.1 NT Homo sapiens mRNA for KIAA1172 protein, partial cds 832 5851 10890 3. 33 5. OE-96 AB032998. 1 NT Homo sapiens mRNA for KIAA1172 protein, partial cds 2545 7510 2. 15 5. OE-96 11416767 NT Homo sapiens phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), mRNA 4748 9733 1. 285. 0E-96 X60812. 1 NT H. sapiens DNA for w 4067 9061 10.5 3.0E-96 H68656.1 EST_HUMAN yr87h12.r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 212327 5' 412 5449 4. 94 2. OE-96 4503098 NT Homo sapiens chondroitin sulfate proteoglycan 4 (melanoma-associated) (CSPG4), mRNA 738 5761 10784 1. 2 2.0E-96 AL163248. 2 NT Homo sapiens chromosome 21 segment HS21 C048 1756 6749 11832 1. 81 2. OE-96 7706205 NT Homo sapiens CGI-201 protein (LOC51340), mRNA 4613 9599 14585 2. 68 2. OE-96 BE148074. 1 EST HUMAN RC3-HT0230-040500-110-g02 HT0230 Homo sapiens cDNA 663 5689 10698 2.97 1.0E-96 Y18890.1 NT Huamn endogenous retrovirus type K (HERV-K), gag, pol and env genes 1743 6738 11814 4. 56 1. OE-96 AW955054. 1 EST HUMAN EST367124 MAGE resequences, MAGC Homo sapiens cDNA 1743 6738 11815 4.56 1.0E-96 AW955054 1 EST HUMAN EST367124 MAGE resequences, MAGC Homo sapiens cDNA 2204 7705 12305 1. 95 1. OE-96 U51472. 2 NT Felis catus superfast myosin heavy chain (sMyHC) mRNA, complete cds 925 5942 10976 3. 84 4. OE-97 BE004436. 1 EST HUMAN CMO-BN0106-170300-293-a06 BN0106 Homo sapiens cDNA 1867 6856 11944 1. 34 4. OE-97 5453572 NT Homo sapiens brefeldin A-inhibited guanine nucleotide-exchange protein 2 (BIG2), mRNA 241 5302 10312 2.28 3.0E-97 AB032998. 1 NT Homo sapiens mRNA for KIAA1172 protein, partial cds 863 5882 10923 9. 5 3.0E-97 4502166 NT Homo sapiens amytoid beta (A4) precursor protein (protease nexin-II, Alzheimer disease) (APP), mRNA 863 5882 10924 9. 5 3.0E-97 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nexin-tl, Alzheimer disease) (APP), mRNA 1415 7747 11473 1. 64 3. OE-97 4758813 NT Homo sapiens N-myc (and STAT) interactor (NMi), mRNA 2371 7706 12462 2.66 3.0E-97 U36255 1 NT Human beta-prime-adaptin (BAM22) gene, exon 7 3186 8202 13224 1.14 3.0E-97 5174478 NT Homo sapiens pericentrin (PCNT) mRNA 4635 9620 14612 35. 24 1.0E-97 4503470 NT Homo sapiens eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) mRNA Page 154 of 209 Tabie 4 Single Exon Probes Expressed in HBL100 Ceils Probe Exon Most Similar Top Hit 'SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Database NO : NO : Value Source. Value s90 5908 10948 6. 71 9. oE-98 BE090973. 1 EST HUMAN PM4-BT0724-010400-008-a12 BT0724 Homo sapiens cDNA 1257 6255 11297 1. 1 9. OE-98 8393092 NT Homo sapiens cat eye syndrome critical region gene 1 (CECR1), mRNA 5021 9992 14966 1. 03 9. OE-98 11419594 NT Homo sapiens FSH primary response (LRPR1, rat) homolog 1 (FSHPRH1), mRNA 24 5104 4. 32 8. OE-98 AJ251158. 1 NT Homo sapiens partial MICB gene for MHC class 1 chain-related protein B, exons 2-3 and joined CDS 1529 6526 1'1584 1. 06 8. OE-98 5031810 NT Homo sapiens IL2-inducible T-cell kinase (ITK), mRNA 1529 6526 11585 1. 06 8. OE-98 5031810 NT Homo sapiens tL2-inducible T-cell kinase (ITK), mRNA 1688 6684 11759 3. 31 8. OE-98 A8017007. 1 NT Homo sapiens PMS2L16 mRNA, partial cds 1688 6684 11760 3. 31 8. OE-98 AB017007. 1 NT 3706 8710 13713 6. 04 8. OE-98 J04469. 1 NT Human mitochondrial creatine kinase (CKMT) gene, complete cds 2113 7093 12207 1. 06 3. OE-98 AJ403124. 1 EST HUMAN AJ403124 3. 4 (downregulated in larynx carcinoma) Homo sapiens cDNA clone IS 2534 7499 12619 1. 4 3. OE-98 AB014607. 1 NT Homo sapiens mRNA for KIAA0707 protein, partial cds 2676 7634 2. 13 3. OE-98 AA077498. 1 EST HUMAN 7B18H01 Chromosome 7 Fetal Brain cDNA Library Homo sapiens cDNA clona 7B18H01 726 5749 10770 2. 43 2. OE-98 BE261694. 1 EST HUMAN 1149486F1 NIH MGC 19 Homo sapiens cDNA clone IMAGE : 3502245 5' 2026 7009 12115 2. 25 2. OE-98 BE294281. 1 EST HUMAN 601172658F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE Homo 3528134 5' 2176 7155 12275 2. 4 2. OE-98 AL163202. 2 NT Homo sapiens chromosome 21 segment HS21 C002 3ggg 8g87 13973 0. 94 2. OE-98 8923308 NT Homo sapiens hypothetical protein FLJ20333 (FLJ20333), mRNA 4172 9167 14154 0. 68 2. OE-98 AF032897. 1 NT Homo sapiens potassium channel subunit (HERG-3) mRNA, complete cds 4214 9207 14186 3. 27 2. OE-98 4758331 NT Homo sapiens fatty-acid-Coenzyme A igase, long-chain 4 (FACL4) mRNA 4680 9665 14646 1. 61 2. OE-98 AF218902. 1 NT Homo sapiens attactin precursor (ATRN) gene, exon 16 _ 4680 9665 14647 1. 61 2. OE-98 AF218902. 1 NT Homo sapiens attractin precursor (ATRN) gene, exon 16 _, 136b04. x1 NCI_CGAP Ut1 Homo sapiens cDNA clone IMAGE : 2261743 3'similar to SW : RL2B HUMAN 403 5439 10459 91. 85 1. OE-98 AI862007. 1 EST HUMAN P29316 60S RIBOSOMAL PROTEIN L23A. ; 452 5489 10504 2. 47 1. OE-98 AW 998611. 1 EST HUMAN PMO-BN0065-100300-001-c06 BN0065 Homo sapiens cDNA yv23f05. r1 Soares fetal liver spleen 1NFLS Homo sapiens cDNA clone IMAGE : 243585 5'similar to 1761 6753 11838 68. 95 1. OE-98 N49818. 1 EST HUMAN PIR : S54204 S54204 ibosomal protein L29-human ;' 2069 7051 12159 1. 5 6. OE-99 11430555 NT Homo sapiens cysteine-rich repeat-containing protein S52 precmsor, (LOC51232), mRNA 2069 7051 12160 1. 5 6. OE-99 11430555 NT Homo sapiens cysteine-rich repeat-containing protein S52 precusor, (LOC51232), mRNA- 4605 9593 14579 1. 1 6. OE-99 4502660 NT Homo sapiens CD34 antigen (CD34) mRNA 1924 6910 12005 0. 92 5. OE-99 Y11365. 1 NT H. sapiens IMPA gene, exon 8 4432 9422 14408 1. 25 5. OE-99 AF009660. 1 NT Homo sapiens T cell receptor beta locus, TCRBV7S3A2 to TCRBV12S2 region _, 4586 9574 14564 1. 82 5. OE-99 AF265555. 1 NT Homo sapiens ubiquiGn-conjugaGng BIR-domain enzyme APOLLON mRNA, completa cds 4586 9574 14565 1. 82 5. OE-99 AF265555. 1 NT Homo sapiens ubiquitin-conjugating BIR-domain enzyme APOLLON mRNA, complete cds W Page 155 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Top Hit Descriptor Value xp09e06. xd1 NCI_CGAP_HN9 Homo sapiens cDNA clone IMAGE : 2739874 3'similar to gb : M31212 MYOSIN 1220 6220 21. 29 2. OE-99 AW274792. 1 EST HUMAN LIGHT CHAIN ALKALI, NON-MUSCLE ISOFORM (HUMAN) ; 3184 8200 13223 2. 08 2. OE-99 M30938. 1 NT Human Ku (p70/p80) subunit mRNA, complete cds Homo sapiens short chain L-3-hydroxyacyl-CoA dehydrogenase precursor (HADHSC) gene, nuclear gene 4412 9402 14387 2. 65 2.0E-99 AF095703. 1 NT encoding mitochondrial protein, complete cds 313 5368 10379 1. 21 1. OE-99 AF114487. 1 NT Homo sapiens intersectin long isoform (ITSN) mRNA, complete cds 378 5425 10440 1. 23 1. OE-99 11526150 NT Homo sapiens GA-binding protein transcription factor, alpha subunit (60kD) (GABPA), mRNA 1396 6393 11447 8. 91 1. OE-99 M30938. 1 NT Human Ku (p70/p80) subunit mRNA, complete cds 1525 6522 11578 3. 27 1.0E-99 AF192523. 1 NT Homo sapiens truncated Niemann-Pick C3 protein (NPC3) mRNA, complete cds 1525 6522 11579 3. 27-. OE-99 AF192523. 1 NT Homo sapiens truncated Niemann-Pick C3 protein (NPC3) mRNA, complete cds 1886 6875 11964 1. 12 1. OE-99 4503730 NT Homo sapiens FK506-binding protein 6 (36kD) (FKBP6) mRNA, and translated products 1886 6875 11965 1. 12 1. OE-99 4503730 NT Homo sapiens FK506-binding protein 6 (36kD) (FKBP6) mRNA, and translated products 3011 8028 13039 1.27 1.0E-99 J03171. 1 NT Human interferon-alpha receptor (HulFN-alphaRec) mRNA, eomplete eds 4255 9249 14233 2. 45 1. OE-99 AF098018. 1 NT Homo sapiens fatty acid amide hydrolase (FAAH) gene, exon 14 4255 9249 14234 2. 45 1. OE-99 AF098018. 1 NT Homo sapiens fatty acid amide hydrolase (FAAH) gene, exon 14 1 5083 10067 1.69 1.0E-100 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21 C047 2 5083 10067 1. 64 1. OE-100 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21 C047 67 5146 10151 1. 24 1. OE-100 11418230 NT Homo sapiens Testis-specific XK-related protein on Y (XKRY), mRNA 67 5146 10152 1. 24 I. OE-100 11418230 NT Homo sapiens Testis-specific XK-related protein on Y (XKRY), mRNA 85 5162 10174 2. 54 1. 0E-100 AW275237. 1 EST-HUMAN xv78b11.x1 NCI_CGAP_Brn53 Homo sapiens cDNA clone IMAGE : 2824605 3' 168 5233 10243 0. 691. 0E-100 AL163206. 2 NT Homo sapiens chromosome 21 segment HS21C006 315 5370 10381 1. 05 1. OE-100 AL163249. 2 NT Homo sapiens chromosome 21 segment HS21 C049 341 5393 10400 1. 8 1. OE-100 T05087. 1 EST HUMAN EST02975 Fetal brain, Stratagene (cat#936206) Homo sapiens cDNA clone HFBCR32 Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat 434 5472 1. 98 1. OE-100 AF003528. 1 NT regions 486 5523 19. 01 1. 0E-100 X89631. 1 NT G. gorilla DNA for ZNF80 gene homolog 506 5541 10547 1. 36 1. OE-100 BE180609. 1 EST HUMAN RC3-HT0625-040500-022-b09 HT0625 Homo sapiens cDNA 1003 6013 11042 2. 46 1. OE-100 7661685 NT Homo sapiens DKFZP586M0122 protein (DKFZP586M0122), mRNA 1003 6013 11043 2. 46 1. OE-100 7661685 NT Homo sapiens DKFZP586M0122 protein (DKFZP586M0122), mRNA 1514 6512 1. 64 1. OE-100 AW207555. 1 EST_HUMAN UI-HBI1-afk-c-07-0-UI.s1 NCI_CGAP_Sub3 Homo sapiens cDNA clone IMAGE : 2722164 3' qf62f09. x1 SoarestestisNHT Homo sapiens cDNA clone IMAGE : 754633 3' similar to SW : CYTCOTJA 1519 6516 11573 1.15 1.0E-100 AI200857. 1 EST HUMAN P81061 CYSTATIN ; 1827 6817 11911 1. 44 1. OE-100 AB032994. 1 NT Homo sapiens mRNA for KIAA1168 protein, partial cds 2634 7594 12707 1.52 1.0E-100 11418976 NT Homo sapiens KIAA0957 protein (KIAA0957), mRNA Page 156 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: NO: Source Value 2952 7971 4.15 1.0E-100 D11078.1 NT Homo sapiens RGH2 gene, retrovirus-like element 4089 9083 14074 1.57 1.0E-100 AF057354.1 NT Homo sapiens myotubularin-related protein 1a mRNA, partial cds 4114 9108 14093 2. 28 1. OE-100 4503792 NT Homo sapiens follicle stimulating hormone receptw (FSHR) mRNA 4920 9898 14872 3. 82 1.0E-100 5032104 NT Homo sapiens small optic lobes (Drosophila) homolog (SOLH) mRNA 4920 9898 14873 3. 82 1. OE-100 5032104 NT Homo sapiens small optic lobes (Drosophila) homolog (SOLH) mRNA 76 5154 10164 1.88 1.0E-101 7110714 NT Homo sapiens SEC14 (S. cerevisiae)-like 2 (SEC14L2), mRNA 76 5154 10165 1. 88 1. OE-101 7110714 NT Homo sapiens SEC14 (S. cerevisiae)-like 2 (SEC14L2), mRNA 677 5702 10710 2. 59 1. OE-101 AB007915. 2 NT Homo sapiens mRNA for KIAA0446 protein, partial cds 694 5718 10735 6. 32 1. OE-101 7110734 NT Homo sapiens ventral anterior homeobox 2 (VAX2), mRNA 694 5718 10736 6. 32 1. OE-101 7110734 NT Homo sapiens ventral anterior homeobox 2 (VAX2), mRNA 762 5783 10811 4. 64 1. OE-101 7657454 NT Homo sapiens pescadillo (zebrafish) homolog 1, containing BRCT domain (PES1), mRNA Homo sapiens phosphoribosyiglycinamide formyttransferase, phosphoribosyiglycinamide synthetase, 843 5862 10902 3. 85 1. OE-101 4503914 NT phosphoribosyiaminoimidazole synthetase (GART) mRNA 970 5985 11020 34. 36 1. OE-101 BF681218. 1 EST HUMAN 602156474F1 NIH MGC 83 Homo sapiens cDNA clone IMAGE : 4297291 5' 1035 6045 11074 1. 9 1.0E-101 AI2218768. 1 ESTHUMAN qg99e09. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 1843336 3' 1548 6546 11606 2. 07 1. OE-101 5921460 NT Homo sapiens butyrophilin, subfamily 2, member A1 (BTN2A1), mRNA 1548 6546 11607 2. 07 1. OE-101 5921460 NT Homo sapiens butyrophilin, subfamily 2, member A1 (BTN2A1), mRNA 1707 6702 11779 1 1. OE-101 7662183 NT Homo sapiens KIAA0569 enge product (KIAA0569), mRNA 1707 6702 11780 1. OE-101 7662183 NT Homo sapiens KIAA0569 gene product (KIAA0569), mRNA 1905 6892 11987 1. 32 1. OE-101 4502996 NT Homo sapiens carboxypeptidase A1 (pancreatic) (CPA1) mRNA 2003 6986 12090 2. 61 1.0E-101 BE843070. 1 EST_HUMAN RC3-ST0281-160600-016-h09 ST0281 Homo sapiens cDNA 2288 7767 12381 0. 97 1.0E-101 5729892 NT Homo sapiens A kinase (PRKA) anchor protein 6 (AKAP6), mRNA 2541 7506 12625 6. 73 1. OE-101 X72993. 1 NT H. sapiens EWS gene, exon 5 2670 7628 12741 5. 15 1. OE-101 AJ237744. 1 NT Homo sapiens RIBIIR gene (partial), exon 12 2670 7628 12742 5. 15 1. OE-101 AJ237744. 1 NT Homo sapiens RIBIIR gene (partial), exon 12 2884 7903 12. 51 1. 0E-101 AJ252312. 1 NT Homo sapiens genomic downstream Rhesus box. 3130 8146 13167 2.4 1.0E-101 4885270 NT Homo sapiens gamma-glutamyitransferase 1 (GGT1) mRNA 3167 8183 2. 76 1. OE-101 BF035327. 1 EST HUMAN 601458531 F1 NIH MGC 66 Homo sapiens cDNA clone IMAGE : 3862086 5' 3296 8307 13332 2. 01 1.0E-101 AW965556. 1 EST HUMAN EST377629 MAGE resequences, MAGI Homo sapiens cDNA 3316 7628 12741 3. 42 1. OE-101 AJ237744. 1 NT Homo sapiens RIBIIR gene (partial), exon 12 3316 7628 12742 3.42 1.0E-101 AJ237744. 1 NT Homo sapiens RIBIIR gene (partial), exon 12 3785 8788 13792 5. 05 1. OE-101 AB022785. 1 NT Homo sapiens ASH2L gene, complete cds, similar to Drosophila ash2 gene 4868 9847 14823 1. 61 1.0E-101 5921460 NT Homo sapiens butyrophilin, subfamily 2, member A1 (BTN2A1), mRNA 4868 9847 14824 1.61 1.0E-101 5921460 NT Homo sapiens butyrophilin, subfamily 2, member A1 (BTN2A1), mRNA Page 157 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top-Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession ORF SEQ Expression (Top) Hit Top Hit Acession EQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLASTE No. NO: NO: source Value 40 5120 10108 0.96 1.0E-102 AF012872.1 NT Homo sapiens phosphatidylinositol 4-kinase 230 (pi4K230) mRNA, complete cds 339 5391 10397 4. 55 1.0E-102 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21CD103 615 5642 10645 0.83 1.0E-102 BE252470. 1 EST HUMAN 601108292F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE : 3344326 5' 766 5787 10816 0. 81 1.0E-102 4557534 NT Homo sapiens down-regulated in adenoma (DRA) mRNA 1100 6107 11137 3.79 1.0E-102 M10976. 1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 1249 6247 11287 1. 82 1. OE-102 11437146 NT Homo sapiens solute carrier family 2 (facilitated glucose transporter), member 9 (SLC2A9), mRNA 1249 6247 11288 1. 82 1. OE-102 11437146 NT Homo sapiens solute carrier family 2 (facilitated glucose transporter), member 9 (SLC2A9), mRNA 1394 6391 11444 373.13 1.0E-102 BE408447. 1 EST HUMAN 601299982F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3629901 5' am60c10. x1 Johnston fronta ! cortex Homo sapiens cDNA clone IMAGE : 1539954 3'similar to 2246 7223 12342 3.39 1.0E-102 AI124669. 1 EST HUMAN SW : GG95_HUMAN Q08379 GOLGIN-95. ; sm60c10.x1 Johnston frontal cortex Homo sapiens cDNA clone IMAGE : 1539954 3'similar to 2246 7223 12343 3.39 1.0E-102 AI124669. 1 EST HUMAN SW : GG95_HUMAN Q08379 GOLGIN-95. ; 2786 78071 12824 0. 69 1.0E-102 11419442 NT Homo sapiens peroxisome biogenesis factor 1 (PEX1), mRNA 2991 8009 13022 1. 4 1.0E-102 7661979 NT Homo sapiens KIAA0187 gene product (KIAA0187), mRNA 3060 8077 13089 2. 88 1. OE-102 AU141005. 1 EST HUMAN AU141005 PLACE4 Homo sapiens cDNA clone PLACE4000650 5' 3060 8077 13090 2. 88 1. OE-102 AU141005. 1 EST HUMAN AU141005 PLACE4 Homo sapiens cDNA clone PLACE4000650 5' 4111 9105 14091 1.63 1.0E-102 AL163207. 2 NT Homo sapiens chromosome 21 segment HS21C007 4287 9279 14267 2. 09 1. OE-102 BE251310. 1 EST HUMAN 601107843F1 NIH MGC 16 Homo sapiens cDNA clone IMAGE : 3343882 5' 4948 9925 14903 1. 17 1. OE-102 R66488. 1 EST HUMAN yi32c04. r1 Soares placenta Nb2HP Homo sapiens cDNA done) IMAGE : 140934 5' 68 5147 10153 1. 86 1.0E-103 BE908158. 1 EST HUMAN 601500405F1 NIH_MGC_70 Homo sapiens cDNA done) IMAGE : 3902305 5' 68 5147 10154 1. 86 1.0E-103 BE908158. 1 EST HUMAN 601500405F1 NIH_MGC 70 Homo sapiens cDNA clone IMAGE : 3902305 5' gg 5176 10186 7.11 1.0E-103 D87078. 2 NT Homo sapiens mRNA for KIAA0235 protein, partial cds 208 5272 10284 9 1.0E-103 5453793 NT Homo sapiens nuclear protein (KKE/D repeat) (NOP56) mRNA 966 5981 11013 0. 87 1. OE-103 AJ278348. 1 NT Homo sapiens mRNA for pregnancy-associated plasma protein-E (PAPPE gene) 1224 6223 11269 8.76 1.0E-103 BE877541. 1 EST HUMAN 601485388F1 NIH_MGC 69 Homo sapiens cDNA clone IMAGE : 3887876 5' 1561 6558 11620 3. 06 1.0E-103 AF012872. 1 NT Homo sapiens phosphatidylinostiol 4-kinase 230 (pi4K230) mRNA, complete cds 1870 6859 11947 0. gg 1.0E-103 7657592 NT Homo saplens smg GDS-ASSOCIATED PROTEIN (SMAP), mRNA 1930 6916 12013 1.28 1.0E-103 4502428 NT Homo sapiens bone morphogenetic protein 8 (osteogenic protein 2) (BMP8) mRNA 1930 6916 12014 1.28 1.0E-103 4502428 NT Homo sapiens bone morphogenetic protein 9 (osteogenic protein 2) (BMP8) mRNA 2242 7219 12338 2.16 1.0E-103 AU134991. 1 EST HUMAN AU134991 PLACE1 Homo sapiens cDNA clone PLACE1000965 5' 2383 7354 12476 1.58 1.0E-103 AF06068. 1 NT Homo sapiens promyelocytic leukemia zinc finger protein (PLZF) gene, complete cds 2547 7512 12630 1. 26 1. 0E-103 N32770. 1 EST HUMAN yw91d08.s1 Soares_placenta_8to9weeks_2nbHP8to9W Homo sapiens cDNA clone IMAGE : 259599 3' 2995 8013 2.58 1.0E-103 BE744722.1 EST-HUMAN 601573113F1 NIH_MGC_9 Homo sapiens cDNA clone IMAGE : 3834315 5' Page 158 of 209 Tabte 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Eression (Top) Hit Top Hit Acession pabase ID NO : Signal BLAST E No. Top Hit Descriptor NO : NO : Value Source, 3295 8306 13331 4. 02 1. OE-103 AW298245. 1 EST HUMAN UI-H-BWO-ajt-h-11-0-UI. s1 NCI CGAP Sub6 Homo sapiens cDNA clone IMAGE : 2733165 3' 3355 8363 13379 1. 23 1. OE-103 AB040892. 1 NT Homo sapiens mRNA for KIAA1459 protein, partial cds 3669 8674 9 1. OE-103 AF023861. 1 NT Macaca mulatta cyclophilin A mRNA, complete cds ab10d12. s1 Stratagene lung (#l937210) Homo sapiens cDNA clone IMAGE : 840407 3'similar to ctains 3704 8708 13711 1. 2 1. OE-103 AA485663. 1 EST HUMAN element LTR10 repetitive element ; 3739 8743 13743 1. 26 1. OE-103 11430876 NT Homo sapiens neuropilin 1 (NRP1), mRNA 3gg7 8gg7 13895 2. 44 1. OE-103 T23683. 1 EST HUMAN seq340 b4HB3MA-Cot109+10-Bio Homo sapiens cDNA clone b4HB3MA-Cot109+10-Bio-7 3' 4646 9631 14626 3. 54 1. OE-103 AL163278. 2 NT Homo sapiens chromosome 21 segment HS21 C078 233 5296 10305 4. 73 1. OE-104 AL037549. 3 EST_HUMAN DKFZp564H1072 r1 564 (synonym : hfbr2) Homo sapiens cDNA clone DKFZp564H1072 5' 233 5296 10306 4. 73 9. OE-104 AL037549. 3 EST HUMAN DKFZp564H10'72 r1 564 (synonym : hfbr2) Homo sapiens cDNA clone DKFZp564H1072 5' 1849 6838 11927 1. 93 1. OE-104 4502428 NT Homo sapiens bone morphogene6c protein 8 (osteogenic protein 2) (BMP8) mRNA zo22c06. s1 Stratagene colon (#l937204) Homo sapiens cDNA clone IMAGE : 587626 3'similar to 2131 7111 12224 5. 4 1. OE-104 AA132975. 1 EST_HUMAN gb : Z14116 rna1 CD59 GLYCOPROTEIN PRECURSOR (HUMAN) ; 2141 7120 12236 5. 95 1. OE-104 BE744628. 1 EST HUMAN 601577460F1 NIH_MGC 9 Homo sapiens cDNA clone IMAGE : 3926438 5' 2308 7283 12402 3. 55 1. OE-104 BF334221. 1 EST HUMAN RC1-CT0249-110900-214-f12 CT0249 Homo sapiens cDNA 2308 7283 12403 3. 55 1. OE-104 BF334221. 1 EST HUMAN RC1-CT0249-110900-214-f12 CT0249 Homo sapiens cDNA 2370 7343 92461 6. 02 1. OE-104 5031570 NT Homo sapiens ARP2 (actin-related protein 2, yeast) homolog (ACTR2), mRNA 2430 7401 12519 1. 36 1. OE-104 7662125 NT Homo sapiens KIAA0440 protein (KIAR0440), mRNA 2430 7401 12520 1. 36 1. OE-104 7662125 NT Homo sapiens KIAA0440 protein (KIAA0440), mRNA 2800 7820 12837 6. 92 1. OE-104 M34671. 1 NT Human lymphocytic antigen CD591MEM43 mRNA, complete cds. 2846 7866 2. 57 1. OE-104 Y11151. 1 NT H. sapiens gene encoding phenylpyruvate tautomerase II 3189 8205 13227 0. 95 1. OE-104 AU133926. 1 EST HUMAN AU133926 OVARC1 Homo sapiens cDNA clone OVARC1000936 5' 3307 8318 1. 84 1. OE-104 AA319436. 1 EST HUMAN EST21658 Adrenal gland tumor Homo sapiens cDNA 5'end 3839 8841 13848 1. 14 1. OE-104 AB032998. 1 NT Homo sapiens mRNA for KIAA1172 protein, partial cds 4008 9004 13992 1. 24 1. OE-104 F11745. 1 EST HUMAN HSC31A071 normalized infant brain cDNA Homo sapiens cDNA clone c-31a07 4252 9246 14230 4. 98 1. OE-104 X02761. 1 NT Human mRNA for fibronectin (FN precursor) 4477 9467 14446 1. 5 1. OE-104 AF231920. 1 NT Homo sapiens chromosome 21 unknown mRNA 4477 9467 14447 1. 5 1. OE-104 AF231920. 1 NT Homo sapiens chromosome 21 unknown mRNA 276 7691 10348 3. 85 1. OE-105 4502166 NT Homo sapiens amyloid beta (A4) precursor protein (protease nepn-il, Alzheimer disease) (APP), mRNA 422 5080 10064 19. 42 1. OE-105 4505150 NT Homo sapiens Meis1 (mousa) homolog (MEIS1) mRNA 589 5620 10618 6. 63 1. OE-105 AF032897. 1 NT Homo sapiens potassium channel subunit (HERG-3) mRNA, complete cds 589 5620 10619 6. 63 1. OE-105 AF032897. 1 NT Homo sapiens potassium channel subunit (HERG-3) mRNA, 1640 6637 5. 46 1. OE-105 AB020981. 1 NT Homo sapiens mRNA for cyclin B2, complete cds L Page 159 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databse Top Hit Descriptor ID NO: Signal RI ASTE No NO: NO: Source Value 1667 6663 11738 1. 04 1. OE-105 AL163247. 2 NT Homo sapiens chromosome 21 segment HS21 C047 1785 6777 11869 1.78 1.0E-105 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21 C080 1885 6874 11963 1. 62 1. OE-105 D50918. 1 NT Human mRNA for KIAA0128 gene, partial cds 2126 7106 12220 5. 92 1. OE-105 AA318369. 1 EST HUMAN EST20609 Spleen I Homo sapiens cDNA 5'end similar to autoimmune antigen Ku, p70/p80 subunit 2260 7237 1. 56 1. OE-105 BE891766. 1 EST_HUMAN 601434491F1 NIH_MGC_72 Homo sapiens cDNA clone IMAGE : 3919511 5' 2649 7609 0. 96 1. OE-105 AA584808. 1 EST_HUMAN no10d05.s1 NCI_CGAP_Phe1 Homo sapiens cDNA clone IMAGE : 1100265 3' 2937 7956 3. 39 1. OE-105 AJ229041. 1 NT Homo sapiens 959 kb contig between AML1 and CBR1 on chromosome 21q22 ; segment 1/3 3252 8265 13286 1. 32 1. OE-105 BF347753. 1 EST HUMAN 602022595F1 NCI CGAP Brn67 Homo sapiens cDNA clone IMAGE : 4158143 5' 3252 8265 13287 1. 32 1. OE-105 BF347753. 1 EST HUMAN 602022595F1 NCI_CGAP Brn67 Homo sapiens cDNA clone IMAGE : 4158143 5' 3981 8979 13964 6.06 1.0E-105 AW961688. 1 EST HUMAN EST373761 MAGE resequences, MAGG Homo sapiens cDNA 4781 9765 4. 42 1. OE-105 AL163208. 2 NT Homo sapiens chromosome 21 segment HS21 C008 4934 9911 14889 0.92 1.0E-105 AB018339. 1 NT Homo sapiens mRNA for Kit0796 protein, partial cds 4974 9950 14928 1.08 1.0E-105 AB020673.1 NT Homo sapiens mRNA for KIAA0866 protein, complete cds 4987 9961 14938 0.67 1.0E-105 AW966015. 1 EST HUMAN EST378088 MAGE resequences, MAGI Homo sapiens cDNA 15052161. 691. OE-106 AW503208. 1 BEST-HUMAN UI-HF-NBO-akt-g-07-0-Ul.r1 NIH_MGC_50 Homo sapiens cDNA clone IMAGE : 3078348 5' 205 5269 10282 2. 72 1. OE-106 A1565065. 1 EST_HUMAN tq79C01.x1 NCI_CGAP_Ut1 Homo sapiens cDNA clone IMAGE : 2215008 3' 537 5572 10576 1. 61 1.0E-106 AW965556. 1 EST HUMAN EST377629 MAGE resequences, MAGI Homo sapiens cDNA 602 5631 10631 8.07 1.0E-106 J00146. 1 NT Human dihydrofdate reductase pseudogene (psi-hd1) 603 5631 10631 6. 16 1.0E-106 J00146. 1 NT Human dihydrofolate reductase pseudogene (psi-hd1) 1492 6490 11545 1. 76 1. OE-106 AF145712. 1 NT Homo sapiens soluble neuropilin-1 mRNA, complete cds 1662 6658 11732 3. 2 1. OE-106 U48724. 1 NT Human epidermal growth factor receptor (EGFR) precursor-mRNA, exon 4, partial cds 1681 6677 11751 2. 79 1. OE-106 U04510. 1 NT Homo sapiens type IV collagen alpha 5 chain (COL4A5) gene, exon 41 ng41cO5. s1 NCI_CGAP_Co3 Homo sapiens cDNA clone IMAGE : 937352 3'similar to contains element 1767 6759 11845 3. 22 1. OE-106 AA527446. 1 EST HUMAN LTR3 repetitive element ; ng41c05.s1 NCI_CGAP_Co3 Homo sapiens cDNA clone IMAGE : 937352 3'similar to contains element 1767 6759 11846 3.22 1.0E-106 AA527446. 1 EST HUMAN LTR3 repetitive element ; 2063 7045 12153 1.25 1.0E-106 BE144286. 1 EST HUMAN MRO-HT0165-140200-008-d10 HT0165 Homo sapiens cDNA 2253 7230 12349 10. 26 1.0E-106 4504184 NT Homo sapiens glutathione S-transferase theta 1 (GSTT1), mRNA Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat 2432 7403 12522 1. 47 1. OE-106 AF003528. 1 NT regions 2525 7491 12611 1 1. OE-106 U64675. 2 NT Homo sapiens sperm membrane protein BS-63 mRNA, complete cds 2527 7493 12613 1. 49 1. OE-106 BE260201. 1 EST_HUMAN 601149783F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 3502461 5' 2683 7641 12757 10 1.0E-106 AI276526. 1 EST HUMAN q176h10. x1 Soares NhHMPu S1 Homo sapiens cDNA clone IMAGE : 1878307 3' 2752 6404 11462 7. 19 1. OE-106 4504184 NT Homo sapiens glutathione S-transferase theta 1 (GSTT1), mRNA Page 160 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databse Top Hit Descriptor ID NO: Signal BLASTE No. NO: NO: Source Value 2752 6404 11463 7.19 1.0E-10-6 4504184 NT Homo sapiens glutathione S-transferase theta 1 (GSTT9), mRNA 2805 7825 12840 1.79 1.0E-106 BE384296. 1 EST HUMAN 601272675F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3613818 5' 2870 7889 12910 4. 45 1. OE-106 AB037747. 1 NT Homo sapiens mRNA for KIAA1326 potein, partial cds 2870 7889 12911 4. 45 1. OE-106 AB037747. 1 NT Homo sapiens mRNA for KIAA1326 protein, partial cds 3107 8123 13141 2. 41 1. OE-106 8922965 NT Homo sapiens hypothetical protein FLJ11273 (FLJ11273), mRNA 3107 8123 13142 2.41 1.0E-106 8922965 NT Homo sapiens hypothetical protein FLJ11273 (FLJ11273), mRNA 3356 8364 13380 0. 99 1. OE-106 AB033104. 1 NT Homo sapiens mRNA for KIAA1278 protein, partial cds 3356 8364 13381 0. 99 1. OE-106 AB033104. 1 NT Homo sapiens mRNA for KIAA1278 protein, partial cds 3714 8718 13719 0.92 1.0E-106 AF001445. 1 NT Homo sapiens core binding factor alpha1 subunit (CBFA1) gene, exon 2 3926 8926 13916 10. 11 1. OE-106 AW974650. 1 EST HUMAN EST386875 MAGE resequences, MAGN Homo sapiens cDNA 3926 8926 13917 10. 11 1. OE-106 AW974650. 1 EST HUMAN EST386875 MAGE resequences, MAGN Homo sapiens cDNA 3944 8942 13932 1. 74 1. OE-106 5729729 NT Homo sapiens APIS-like 1 (API5L1), mRNA 4470 9460 14439 0. 67 1. 0E-106 BE144286. 1 EST HUMAN MRO-HT0165-140200-008-d10 HT0165 Homo sapiens cDNA 4741 9726 14711 1. 39 1. OE-106 U31520. 1 NT Human alpha mannosidase II mRNA, complete cds 234 5297 3. 3 1. OE-107 AJ271735. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 1/2 262 5321 1. 47 1. OE-107 X60459. 1 NT Human IFNAR gene for interferon alphalbeta receptor 624 5651 10655 2. 22 1. OE-107 AF155103. 1 NT Homo sapiens NY-REN-25 antigen mRNA, partial cds 803 5824 10854 1. 94 1. OE-107 X60459. 1 NT Human IFNAR gene for interferon alpha/beta receptor 875 5893 10934 1. 25 1.0E-107 X60459. 1 NT Human IFNAR gene for interferon alpha/beta receptor 954 5970 11003 8. 11. 0E-107 AF154121. 1NT Homo sapiens sodium-dependent high-affinity dicarboxytate transporter (NADC3) mRNA, complete cds 1259 6257 11300 2. 22 1. OE-107 AB032253. 1 NT Homo sapiens BAZ1B mRNA for bromodomain adjacent to zinc finger domain 1B complete cds 1536 6534 11593 8.86 1.0E-107 BF087405, 1 ESTHUMAN QV2-HT0540-120900-358-a05HT0540 Homo sapiens cDNA 1713 6708 11784 4. 61 1.0E-107 AF136275. 1 NT Homo sapiens cathepsin Z precursor (CTSZ) gene, exon 3 1804 6795 11885 2. 87 1. OE-107 AB007922. 2 NT Homo sapiens mRNA for KIAA0453 protein, partial cds 1804 6795 11886 2.87 1.0E-107 AB007922. 2 NT Homo sapiens mRNA for KIAA0453 protein, partial cds 2146 7125 12242 1. 06 1. OE-107 U13729. 1 NT Human dipeptidyi peptidase IV (CD26) gene, exon 20 2301 7276 12395 4. 22 1.0E-107 AW842451. 1 ESTHUMAN PM1-CN0031-190100-001-d03CN0031 Homo sapiens cDNA 2301 7276 12396 4. 22 1.0E-107 AW842451. 1 ESTHUMAN PM1-CN0031-190100-001-d03CN0031 Homo sapiens cDNA 2463 7432 12550 1.65 1.0E-107 BE732460. 1 EST HUMAN 601567619F1 NIH MGC 21 Homo sapiens cDNA clone IMAGE : 3842309 5' 2463 7432 12551 1.65 1.0E-107 BE732460. 1 EST_HUMAN 601567619F1 NIH_MGC 21 Homo sapiens cDNA clone IMAGE : 3842309 5' 2938 7957 12974 3.71 1.0E-107 AW842451. 1 EST HUMAN PM1-CN0031-190100-001-d03 CN0031 Homo sapiens cDNA 2938 7957 12975 3. 71 1. OE-107 AW842451. 1 EST_HUMAN PM1-CN0031-190100-001-dO3 CN0031 Homo sapiens cDNA 3026 8043 13052 7.53 1.0E-107 5902097 NT Homo sapiens SMT3 (suppressor of mif two 3, yeast) homolog 2 (SMT3H2), mRNA Page 161 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databse Top Hit Descriptor ID NO: Signal RI ASTE No Valut Vaque 3736 8740 13739 5. 33 1. 0E-107 AF020671. 1 NT Homo sapiens myotubularin (MTM1) gene, exon 9 940 5957 10990 2. 88 1. 0E-108 BE296042. 1 EST_HUMAN 601177018F1 NIH_MGC_17 Homo sapiens cDNA done) IMAGE : 3532348 5' 1246 6244 11284 5. 83 1. 0E-108 Y18000. 1 NT Homo sapiens NF2 gene 2025 7008 12114 1. 65 1. OE-108 BF026728. 1 EST HUMAN 601671914F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3954939 5' bb25b10.x1 NIH-MGC-14 Homo sapiens cDNA clone IMAGE : 2963899 3'similar to gb : X53777 60S 2360 7334 12451 12.25 1.0E0-108 BE206694.1 EST_HUMAN RIBOSOMAL PROTEIN L23 (HUMAN); gb:J06277 Mouse hexokinase mRNA, complete cds (MOUSE); hi12a11.x1 NCI_CGAP_GU1 Homo sapiens cDNA clone IMAGE:2972060 3' similar to SW:3BP1 MOUSE 4033 9029 14017 1. 15 1. 0E-108 AW664438. 1 EST HUMAN P55194 SH3-BINDING PROTEIN 3BP-1. ; 4394 9385 14367 3. 04 1. OE-108 U72961. 1 NT Human hepatocyte nuclear factor 4-alpha gene, exon 2 4394 9385 14368 3. 04 1. OE-108 U72961. 1 NT Human hepatocyte nuclear factor 4-alpha gene, axon 2 4659 9644 14632 3. 68 1. 0E-108 7661979 NT Homo sapiens KfAA0187 gene product (KIM0187), mRNA 4765 9749 14737 0. 88 1. 0E-108 AW504799. 1 EST_HUMAN UI-HF-BNO-aln-e-04-0-ul.r1 NIH-MGC_50 Homo sapiens cDNA clone IMAGE : 3080166 5' 4791 9775 14759 2. 91 1. OE-108 AJ008005. 1 NT Homo sapiens PSN1 gene, alternative transcript 4962 9939 14916 0. 95 1. OE-108 5031624 NT Homo sapiens CCAAT-box-binding transcription factor (CBF2) mRNA 4988 9962 14939 0. 72 1. OE-108 Y12490. 1 NT Homo sapiens mRNA for Gogli-associated microtuble-binding protein (GMAP-210) 43 5123 10111 2. 46 1. OE-109 AW803116. 1 EST HUMAN IL2-UM0077-260400-079-D06 UM0077 Homo sapiens cDNA 66, 5145 10150 3. 94 1. 0E-1-9 D86974. 1 NT Human mRNA for KIAA0220 gene, partial cds 218 5281 10292 0. 76 1. OE-109 11422486 NT Homo sapiens hypothetical protein FLJ11316 (FLJ11316), mRNA 226 5288 10296 7. 69 1. 0E-109 11438391 NT Homo sapiens reticulocalbin 1, EF-hand calcium binding domain (RCN1), mRNA 464 5501 10511 9. 2 1. OE-109 4507712 NT Homo sapiens tetratricopeptide repeat domain 2 (TTC2) mRNA 593 5624 10623 15. 28 1. OE-109 AB023216. 1 NT Homo sapiens mRNA for KIAA0999 protein, partial cds 593 5624 10624 15. 28 1. OE-109 AB023216. 1 NT Homo spaiens mRNA for KIAA0999 protein, partial cds 1183 6185 11222 93. 77 1.0E-109 M28699. 1 NT Homo sapiens nucleolar phosphoprotein B23 (NPM1) mRNA, complete cds 1184 6185 11222 72. 21 1. OE-109 M28699. 1 NT Homo sapiens nucleolar phosphoprotein B23 (NPM1) mRNA, complete cds 1510 6508 11564 1. 52 1. 0E-109 BE293673. 1 EST HUMAN 601186922F2 NIH MGC 15 Homo sapiens cDNA clone IMAGE : 2959636 5' 1510 6508 11565 1. 52 1. 0E-109 BE293673. 1 EST-HUMAN 60118692F2 NIH_MGC_15 Homo sapiens cDNA clone IMAGE : 2959636 5' 1836 6826 11914 5. 54 1. OE-109 D13643. 2 NT Homo sapiens mRNA for KIAA018 protein, partial cds 2181 7160 12280 2. 32 1. OE-109 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 2189 7168 12288 4. 31 1. OE-109 Y17123. 1 NT Homo sapiens SNF5/INl1 gene, exon 6 ow95aO1. x1 Soare_fetal_liver_spleen_1NFLS_S1 Homo sapiens cDNA clone IMAGE : 1654536 3'similar to 2549 7514 126324. 09 1. 0E-109 AI022328. 1 EST HUMAN TR : 002197 002197 CIRCULATING CATHODIC ANTIGEN. ; ow95a01. x1 SoaresfetaUiversp ! een1NFLSS1 Homo sapiens cDNA clone IMAGE : 1654536 3'similar to 2549 7514 12633 4. 09 1. 0E-109 AI022328. 1 EST HUMAN TR : 002197 002197 CIRCULATING CATHODIC ANTIGEN. ; Page 162 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORS SET Expression (Top) Hit Top Hit Acessoin Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Top Hit Descriptor NO: NO: Source Value 2550 7515 12634 2.41 1.0E-109 4504206 NT Homo sapiens guanylate cyclase activator 1A 9retina) (GUCA1A) mRNA J2816F Human fetal heart, Lambda ZAP Express Homo sapiens cDNA clone J2816 5'similar to ZINC 2984 8002 13014 1. 32 1. OE-109 N85190. 1 EST HUMAN FINGER PROTEIN ZNF43 3304 8315 13341 1. 43 1. 0E-109 AW893192. 1 ESTHUMAN CM3-NN0009-190400-150-f10 NN0009 Homo sapiens cDNA 3304 8315 13342 1. 43 1. OE-109 AW893192. 1 EST-HUMAN CM3-NN0009-190400-150-f10 NN0009 Homo sapiens cDNA 3440 8448 13474 1 1. OE-109 AF240698. 1 NT Homo sapiens retinol dehydrogenase homolog isoform-1 (RDH) mRNA, complete cds 3751 8755 2. 78 1. OE-109 BE146144. 1 EST_HUMAN MR0-HT0209-110400-108-aO4 HT0209 Homo sapiens cDNA ts98e06. x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2239330 3'similar to WP : F53A2. 8 4023 9019 14006 4. 42 1. OE-109 AI655417. 1 EST-HUMAN CE16100 ; nu93c12. s1 NCI_CGAP_Pr22 Homo sapiens cDNA clone IMAGE : 1218262 3'similar to SW : GTT2HUMAN 4038 9034 14022 0. 94 1. OE-109 AA662274. 1 EST HUMAN P30712 GLUTHATHIONE S-TRANSFERASE THETA 2 ; nu93c12. s1 NCI_CGAP_Pr22 Homo sapiens cDNA clone IMAGE:1218262 3'similar to SW : GTT2HUMAN 4038 9034 14023 0. 94 1. 0E-109 AA662274. 1 |EST_HUMAN P30712 GLUTHATHIONE S-TRANSFERASE THETA 2 ; 4280 9273 14262 2. 85 1. OE-109 4504206 NT Homo sapiens guanylate cyclase activator 1A (retina) (GUCA1A) mRNA 4469 9459 14438 1. 07 1. 0E-109 7662083 NT Homo sapiens KIAA0377 gene product (KIAA0377), mRNA 3 5084 10068 0. 8 1. OE-110 7549804 NT Homo sapiens deiodinase, iodothyronine, type 11 (DI02), transcript variant 2, mRNA 38 5118 10105 3. 85 1. OE-110 5803073 NT Homo sapiens leucine-zipper-liek transcriptional regulator, 1 (LZTR1), mRNA 38 5118 10106 3. 85 1. 0E-110 5803073 NT Homo sapiens leucine-zipper-like transcriptional regulator, 1 (LZTR1), mRNA 109 5084 10068 0. 79 1. OE-110 7549804 NT Homo sapiens deiodinase, iodothyronine, type 11 (DI02), transcript variant 2, mRNA 292 5349 10362 0.83 1.0E-110 D87291.1 NT Human mRNA for inward rectifier potassium ohannel, complete cds 523 5558 10561 0. 78 1. 0E-110 U84550. 1 NT Human dystrobrevin (DTN) gene, exon 20 1161 6165 11199 0. 8 1. OE-110 5031620 NT Homo sapiens calcitonin receptor-like (CALCRL) mRNA 1260 6258 11301 1. 42 1. OE-110 AB032253. 1 NT Homo sapiens BAZ1 B mRNA for bromodomain adjacent to zinc finger domain 1 B, complete cds 1879 6868 11957 0. 92 1. 0E-110 BE379477. 1 EST-HUMAN 601237545F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE : 3609683 5' 2005 6988 1. 45 1. 0E-110 BF508896. 1 EST HUMAN Ul-H-BI4-aos-b-05-0-Ul. s1 NCI CGAP Sub8 Homo sapiens cDNA clone IMAGE : 3085784 3' 2767 7788 2 1.0E-110 4503098 NT Homo sapiens chondrotin sulfate proteoglycan 4 (melanoma-assoicated0 (CSPG40, mRNA 2961 6258 11301 1.29 1.0E-110 AB032253.1 NT Homo sapiens BAZ1 mRNA for bromodomain adjacent to zinc finger dimain 18, complete cds Homo sapiens Bruton's tyrosine kinase (BTK), alpha-D-galactosidase A (GLA), L44-like ribosomal protein 3013 8030 1. 07 1. 0E-110 U78027. 1 NT (L44L) and FTP3 (FTP3) genes, complete cds 4080 9074 14062 2. 55 1. 0E-110 M15918. 1 NT Human autoimmune antigen small nuclear ribonucleoprotein E pseudogene ou32b10. x1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 1627963 3'similar to 4500 9490 14466 2. 09 1. OE-110 A1017213. 1 EST HUMAN SW : N121RAT P52591 NUCLEAR ENVELOPE PORE MEMBRANE PROTEIN POM 121 ; 4520 9510 14494 3.28 1.0E-110 AU117812. 1 EST_HUMAN AU117812 HEMBA1 Homo sapiens cDNA clone HEMBA1002241 5' 4814 9798 2. 3 1. 0E-110 7662441 NT Homo sapiens KIAA1002 protein (KIAA1002), mRNA Page 163 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession Top Hit Descriptor ID NO : Signal BLAST E No_ Database NO : NO : Source Value 174 5238 43. 9 1. OE-111 U43701. 1 NT Human ibosomal protein L23a mRNA, complete cds 1g6 5260 10273 1. 07 1. OE-111 4758807 NT Homo sapiens ras GTPase acfivating protein-like (NGAP) mRNA 725 5748 2. 71 1. OE-111 BF035327. 1 EST HUMAN 601458531 F1 NIH MGC 66 Homo sapiens cDNA clone IMAGE : 3862086 5' 734 5757 10779 3. 66 1. OE-111 8393092 NT Homo sapiens cat eye syndrome critical region gene 1 (CECR1), mRNA 914 5930 10965 2. 29 1. OE-111 M25142. 1 NT Human cardiac alpha-myosin heavy chain (MYH6) gene, exons 32 to 34 1589 6586 11648 1. 57 1. OE-111 7662177 NT Homo sapiens KIAA0555 gene product (KIAA0555), mRNA 4047 9043 14032 1. 17 1. OE-111 7661569 NT Homo sapiens DKFZP434D156 protein (DKFZP434D156), mRNA 4203 9196 14178 4. 64 1. OE-111 K02268. 1 NT Human enkephalin B (enkB) gene, exon 4 and 3'flank and complete cds 605 5632 10632 0. 87 1. OE-112 4501854 NT Homo sapiens acetyl-Coenzyme A carboxylase beta (ACACB), mRNA 606 5633 10633 4. 82 1. OE-112 U29103. 1 NT Human steroidogenic acute regulatory protein (StAR) gene, exon 5 606 5633 10634 4. 82 1. OE-112 U29103. 1 NT Human steroidogenic acute regulatory protein (StAR) gene, exon 5 626 5653 10657 1. 33 1. OE-112 BF509039. 1 EST HUMAN UI-H-BI4-aot-g-04-0-UI. s1 NCI_CGAP Sub8 Homo sapiens cDNA clone IMAGE : 3086023 3' 626 5653 10658 1. 33 1. OE-112 BF509039. 1 EST HUMAN UI-H-BI4-aot-g-04-0-UI. s1 NCI CGAP Sub8 Homo sapiens cDNA clone IMAGE : 3086023 3' 986 6001 11032 1. 84 1. OE-112 AF157623. 1 NT Homo sapiens HTRA serine protease (PRSS11) gene, complete cds 1045 6055 11083 2. 53 1. OE-112 P52742 SW ISSPROT ZINC FINGER PROTEIN 135 1645 6641 11711 3. 11 1. OE-112 7662125 NT Homo sapiens KIAA0440 protein (KIAA0440), mRNA 1645 6641 11712 3. 11 1. OE-112 7662125 NT Homo sapiens KIAA0440 protein (KIAA0440), mRNA 2436 7407 12524 1. 26 1. OE-112 BE866859. 1 EST HUMAN 601442674F1 NIH_MGC 65 Homo sapiens cDNA clone IMAGE : 3846858 5' 3004 8022 0. 72 1. OE-112 4504116 NT Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1) mRNA 3171 8187 13208 1. 07 1. OE-112 BE083092. 1 EST HUMAN RC2-BT0642-030400-021-d09 BT0642 Homo sapiens cDNA 3171 8187 13209 1. 07 1. OE-112 BE083092. 1 EST HUMAN RC2-ST0842-030400-021-d09 BT0642 Homo sapiens cDNA 3790 8793 13798 0. 71 1. OE-112 BE076073. 1 EST HUMAN MR2-BT0590-090300-113-f09 BT0590 Homo sapiens cDNA 4608 9595 14580 5. 12 1. OE-112 AB037832. 1 NT Homo sapiens mRNA for KIAA1411 protein, partial cds 4608 9595 14581 5. 12 1. OE-112 AB037832. 1 NT Homo sapiens mRNA fa KIAA1411 protein, partial cds = 733 5756 10777 7. 13 1. OE-113 AI365586. 1 EST HUMAN ao95f01. x1 Schiller meningioma Homo sapiens cDNA clone IMAGE : 1953625 3'__ 733 5756 10778 7. 13 1. OE-113 AI365586. 1 EST HUMAN ao95f01. x1 Schiller meningioma Homo sapiens cDNA clone IMAGE : 1953625 3' g28 5945 10979 7. 7 1. OE-113 M11965. 1 NT Human X linked phosphoglycerate kinase gene, exon 8 1509 6507 11563 3. 94 1. OE-113 AI365586. 1 EST HUMAN ao95f01. x1 Schiller meningioma Homo sapiens cDNA clone IMAGE : 1953625 3' ggg 7702 11980 1. 45 1. OE-113 AF240775. 1 NT Homo sapiens eIF4E-transporter mRNA, complate cds 3057 8074 13087 2. 19 1. OE-113 AJ223948. 1 NT Homo sapiens mRNA fa putative RNA helicase, 3'end 59 5139 10138 0. 76 1. OE-114 Y17151. 2 NT Homo sapiens mRNA for mulfidrug resistance protein 3 (ABCC3) 59 5139 10139 0. 76 1. OE-114 Y17151. 2 NT Homo saPiens mRNA fa multidrug resistance protein 3 (ABCC3) 59 5139 10140 0. 76 1. OE-114 Y17151. 2 NT Homo sapiens mRNA fa multidrug resistance protein 3 (ABCC3) Page 164 of 209 Table 4 Single Exon Probes Expressed in NBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No, Database Top Hit Descriptor NO : NO : Source, Value yd15c01. s1 Soares fetal liver spleen 1 NFLS Homo sapiens cDNA clone IMAGE : 108288 3'similar to 637 5665 10669 5. 07 1. OE-114 T70551. 1 EST HUMAN gb : A21187 ALPHA-2-MACROGLOBULIN PRECURSOR (HUMAN) ; contains Alu repetitive element ; 1054 6063 11093 3. 1 1. OE-114 8923087 NT Homo sapiens hypothe6cal protein FLJ20080 (FLJ20080), mRNA 1294 6292 11338 3. 85 1. OE-114 7657529 NT Homo sapiens rhabdoid tumor dele6on region protein 1 (RTDR1), mRNA 1602 6598 19659 5. 21 1. OE-114 6631094 NT Homo sapiens minichromosome maintenance deficient (S. cerevisiae) 3 (MCM3), mRNA 1633 6630 11699 11. 15 1. OE-114 6679073 NT Homo sapiens nucleoporin-like protein 1 (N ! P 1), mFtNA 2194 7173 12294 1. 31 1. OE-114 AB002374. 1 NT Human mRNA for KIAA0376 gene, partiai cds 2732 5125 10114 1. 1 1. OE-114 AB033102. 1 NT Homo sapiens mRNA for KIAA1276 protein, partial cds 2732 5125 10115 1. 1 1. OE-114 AB033102. 1 NT Homo sapiens mRNA for KIAA1276 protein, partial cds 3058 8075 13088 2. 52 1. OE-114 X04086. 1 NT Human gene for catalase (EC 1. 11. 1. 6) exon 2 mapping to chromosome 11, band p13 3098 8114 13132 1. 9 1. OE-114 BF206374. 1 EST HUMAN 601869932F1 NfH MGC 19 Homo sapiens cDNA clone IMAGE : 4100214 5' 3909 8909 13905 1. 56 1. OE-114 AF149773. 1 NT Homo sapiens NOD1 protein (NOD1) gene, exons 1, 2, and 3 4266 9259 14249 1. 12 1. OE-114 J03171. 1 NT Human interteron-alpha receptor (HuIFN-alpha-Rec) mRNA, complete cds 4964 9941 14918 1. 66 1. OE-114 BE275324. 1 EST HUMAN 601122173F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3346099 5' 22 5102 10087 91. 8 1. OE-115 4758111 NT Homo sapiens HLA-B associated transcript-1 (D6S81 E) mRNA 130 5196 10211 2. 72 1. OE-115 4505938 NT Homo sapiens po (ymerase (RNA) II (DNA directed) polypeptide A (220kD) (POLR2A) mRNA 134 5200 21. 76 1. OE-115 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA 290 5347 10359 4. 49 1. OE-115 AW 804759. 1 EST HUMAN QV4-UM0094-300300-156-b08 UM0094 Homo sapiens cDNA 778 5799 10828 1. 42 1. OE-115 5174702 NT Homo sapiens transforming growth factor beta-activated kinase-binding protein 1 (TAB1), mRNA 778 5799 10829 1. 42 9. OE-115 5174702 NT Homo sapiens transfwming growth factor beta-activated kinase-binding protein 1 (TAB1), mRNA 780 5801 10831 83. 62 1. OE-115 4503794 NT Homo sapiens ferritin, heavy polypePtide 1 (FTH1) mRNA 1528 6525 91582 1. 41 1. OE-115 AF229180. 1 NT Homo sapiens alpha-aminoadipate semialdehyde synthase mRNA, complete cds 1528 6525 11583 1. 41 1. OE-115 AF229180. 1 NT Homo sapiens alpha-aminoadipate semialdehyde synthase mRNA, complete cds Homo sapiens Brutori s tyrosine kinase (BTK), alpha-D-galactosidase A (GLA), L44-like ribosomal protein 1805 6796 11887 0. 93 1. DE-115 U78027. 1 NT (L44L) and FTP3 (FTP3) genes, complete cds 2027 7010 12116 1. 38 1. OE-115 BE745469. 1 EST HUMAN 601579838F1 NIH MGC 9 Homo sapiens cDNA clone IMAGE : 3928832 5' 2027 7010 12117 1. 38 1. OE-115 BE745469. 1 EST HUMAN 601579838F1 NIH_MGC 9 Homo sapiens cDNA clone IMAGE : 3928832 5' 2236 7213 12330 3. 07 1. OE-115 AF231124. 1 NT Homo sapiens testican-1 mRNA, complete cds 2, 22 1. OE-115 AW 804759. 1 EST HUMAN QV4-UM0094-300300-156-b08 UM0094 Homo sapiens cDNA 3041 8058 13067 7. 57 9. OE-195 AJ245922. 1 NT Homo sapiens mRNA for alpha-tubulin 8 (TUBA8 gene) 3041 8058 13068 7. 57 1. OE-115 AJ245922. 1 NT Homo sapiens mRNA for alpha-fubulin 8 (TUBA8 genej 3392 8400 13426 2. 06 1. OE-115 AJ277892. 1 NT Homo sapiens partial TTN gene for tiBn 3g2g gg29 13920 5. 04 1. OE-115 AB002348. 2 NT Homo sapiens mRNA for KIAA0350 protein, partial cds Page 165 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Single Exon Probes p Most Similar Probe Exon ORF SEQ Expressinoi (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor Value 4144 9139 14123 0.96 1.0E-115 AL137163. 1 NT Novel human gene mapping to chomosome X 4278 9271 14260 1. 49 1. OE-11 691265E NT Homo sapiens sir2-like 3 (SIRT3), mRNA 4313 9305 14289 3.78 1.0E-115 4758279 NT Homo sapiens EphA4 (EPHA4) mRNA 4459 9449 14429 0.91 1.0E-115 8922435 NT Homo sapiens hypothetical protein FLJ10466 (FLJ10466), mRNA 4459 9449 14430 0.91 1.0E-115 8922435 NT Homo sapiens hypothetical m 4539 9529 14515 2. 4 1.0E-115 AL096857. 1 NHT Novel human mRNA from chromosome 1, which has similarities to BAT2 genes 4539 9529 14516 2. 4 1. OE-115 AL096857. 1 NT Novel human mRNA from chromosome 1, which has similarities to BAT2 genes 4750 9735 14721 2. 89 1. OE-115 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21 C068 4750 9735 14722 2. 89 1. OE-115 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21 C068 567 5600 10599 1. 39 1. OE-116 BE275502. 1 EST_HUMAN 601121347F1 NIH_MGC_20 Homo sapiens cDNA clone IMAGE : 2988875 5' 792 5813 10843 1. 45 1. OE-116 4507334 NT Homo sapiens synaptojanin 1 (SYNJ1), mRNA 1951 6937 12038 2. 12 1. OE-116 5174478 NT Homo sapiens pericentrin (PCNT) mRNA 1951 6937 12039 2. 12 1. OE-116 5174478 NT Homo sapiens pericentrin (PCNT) mRNA 1975 6960 12066 1. 37 1. OE-116 AU133080. 1 EST HUMAN AU133080 NT2RP4 Homo sapiens cDNA clone NT2RP4001228 5' 2040 7761 12131 1 1.0E-116 M19824.1 NT Human apolipoprotein B-100 (apoB) gene, exons 17 and 18 2040 7761 12132 1. 0E-11E M19824. 1 NT Human apolipoprotein B-100 (apoB) gene, exons 17 and 18 2244 7221 12340 1. 16 1. OE-116 5453941 NT Homo sapiens protein phosphatase, EF hand calcium-binding domain 1 (PPEF1) mRNA Human olfactory receptor olfr17-201-1 (OR17-201-1) gene, olfactory receptor olfr17-32 (OR17-32) gene and 2279 7255 1. 38 1. OE-116 U78308. 1 NT olfactory receptor pseudo olfr17-01 (OR17-01) pseudogene, complete cds 2387 7358 12480 1. 98 1. OE-116 AB018333. 1 NT Homo sapiens mRNA for Kit0790 protein, partial cds 2660 7710 12731 6 1. OE-116 BE889256. 1 EST_HUMAN 601513337F1 NIH_MGC_71 Homo sapiens cDNA clone IMAGE : 3914600 5' 3101 8117 13134 5. 82 1. OE-116 L77570. 1 NT Homo sapiens DiGeorge syndrome critical region, centromeric end 3101 8117 13135 5. 82 1. OE-116 L77570. 1 NT Homo sapiens DiGeorge syndrome critical region, centromeric end 4253 9247 14231 2. 06 1. OE-116 5031954 NT Homo sapiens sodium phosphate transporter 3 (NPT3) mRNA 4707 9692 14675 1. 27 1. OE-116 A1907096. 1 EST HUMAN PM-BT135-070499-016 BT135 Homo sapiens cDNA 4995 9967 14945 0.95 1.0E-116 AJ243213. 1 NT Homo sapiens partial 5-HT4 receptor gene, exons 2 to 5 554 5588 10589 1. 21 1. OE-117 4826636 NT Homo sapiens acetyl-Coenzyme A carbxylase alpha (ACACA), mRNA 1061 7738 11101 2. 4 1.0E-117 AF124393. 1 NT Mus musculus fragile-X-related protein 1 (Fxr1 h) gene, exons 13a through 15 1714 6709 11785 3. 05 1.0E-117 AF123320. 1 NT Homo sapiens lymphocyte activation-associated protein mRNA, complete cds 1795 6786 11876 1. 83 1. OE-117 M19816. 1 NT Human aplipoprotein B-100 (apoB) gene, exon 10 2149 7128 12245 1.3 1.0E-117 AW957699. 1 EST HUMAN EST369769 MAGE resequences, MAGE Homo sapiens cDNA 2537 B7502 12621 1.04 1.0E-117 M63468. 1 NT Human alpha-5 collagen type IV gene, exon 5 3') 94 821013232 1. 93 1. OE-117 AA978114. 1 ESTHUMAN op32c11. s1 SoaresNFLTGBCS1 Homo sapiens cDNA clone IMAGE : 1578548 3' Page 166 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databse Top Hit Descriptor ID NO : Signal BLAST E No. Sourie Value EST188414 HCC cell line (matastasis to liver in mouse) II Homo sapiens cDNA 5'end similar to ribosomal 3884 8885 13885 14. 06 1. OE-117 AA316723. 1 EST HUMAN protein L29 4218 9211 14190 2. 65 1.0E-117 8659564 NT Homo sapiens collagen, type IV, alpha 5 (Alport syndrome) (COL4A5), mRNA 4444 9434 14417 2. 36 1. OE-117 AL042120. 1 ESTHUMAN DKFZp434C1120r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434C1120 5' 4584 9572 14561 1.11 1.0E-117 X89670. 44584 9572 14562 1 NT H. sapiens mRNA for TPCR16 protein 4665 9650 14639 11.6 1.0E-117 AF134304, 1. 11 1. OE-117 X89670. 1 NT H. sapiens mRNA for TPCR16 protein 4665 9650 14638 11. 6 1. OE-117 AF134304. 2 NT Homo sapiens Scar2 (SCAR2) gene, partial cds KIAA0866 2 NT Homo sapiens Scar2 (SCAR2) gene, partial cds 4782 9766 14750 3. 85 1. 0E-117 AB020673. 1 NT Homo sapiens mRNA for HSPC151 mRNA, protein, complete cds 69 5148 10155 11. 76 1. OE-118 AF161500. 1 NT Homo sapiens 94 5171 10181 1.94 1.0E-118 AL045854. complete cds EST_HUMAN DKFZp434 056_R1 1 24 1.0E-118 7657016 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp4341056 5' 513 5548 10553 7. 2171 7150 12267 7.75 1.0E-118 BE389705.1 EST-HUMAN 601281947F1 NIH_MGC_44 Homo sapiens cDNA clone IMAGE NT Homo sapiens hypothetical protein (DJ328E19. C1. 1), mRNA 903 7734 10957 6. 59 1. OE-118 5174680 NT Homo sapiens sine oculis homeobox (Drosophila) homolog 1 (SIX1) mRNA 2171 7150 12268 7.75 1.0E-118 BE389705, : 3604019 5' EST_HUMAN 601281947F1 NIH_MGC_44 1 2171 7150 12269 7.75 1.0E-118 BE389705. Homo sapiens cDNA clone IMAGE : 3604019 5' EST_HUMAN 601281947F1 NIH_MGC_44 1 2268 7245 1.9 1.0E-118 AW951729. Homo sapiens cDNA clone IMAGE : 3604019 5' 3122 8138 13159 4.93 1.0E-118 AI347694. 1 EST HUMAN EST363799 MAGE resequences, MAGB Homo sapiens cDNA 2667 7626 12738 3. 94 1. OE-118 U07000. 1 NT Human breakpoint cluster region (BCR) gene, complete cds 2667 7626 12739 3. 94 1. OE-118 U07000. 1 NT Human breakpoint cluster region (BCR) gene, complete cds 3031 8048 4. 44 1. OE-118 Y13932. 1 NT Homo sapiens PRKY exon 7 3122 8138 13160 4.93 1.0E-118 AI347694. 1 ESTHUMAN qp01f05. x1 NCiCGAPKid5 Homo sapiens cDNA clone IMAGE : 1916769 3' EST_HUMAN qp01f05.x1 NCI_CGAP_Kid5 Homo sapiens cDNA clone IMAGE:1916769 3' 1 3972 8970 13958 17.63 1.0E-118 (KIAA0478), D23660. 1 NT Human mRNA for ribosomal protein, complete cds 4569 9557 14545 1. 42 1. OE-118 11425793 NT Homo sapiens KIAA0478 gene product 748 5770 10797 0.93 1.0E-119 AF170492. mRNA 1893 6881 11972 3.42 1.0E-119 AB023147. 1 NT Homo sapiens chloride channel CLC4 (CIC4) mRNA, complete cds 1020 7737 11059 4. 3 1. OE-119 7705607 NT Homo sapiens CGI-105 protein (LOC51011), mRNA KIAA0930 1 NT Homo sapiens mRNA for 3029 8046 13056 0.92 1.0E-119 protein, partial cds 8922205 NT Homo sapiens hypothetical protein FLJ10052 (FLJ10052), mRNA 3847 8849 13857 0. 86 1. OE-119 4504116 NT Homo sapiens glutamate receptor, ionotropic, kainate 1 (GRIK1) mRNA 299 5356 10368 1.07 1.0E-120 4507334 NT Homo sapiens synaptojanin 1 (SYNJ1), mRNA 1024 6034 11065 2. 23 1. OE-120 AF248540. 1 NT Homo sapiens intersectin 2 (SH3D1B) mRNA, complete cds 1024 6034 11066 2. 23 1. OE-120 AF248540. 1 NT Homo sapiens intersectin 2 (SH3D1 B) mRNA, complete cds 1400 6397 11452 6. 07 1. OE-120 N44873. 1 EST-HUMAN yy40g12.r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE:273766 5' 1566 6563 11626 4. 73 1.0E-120 AF167706. 1 NT Homo sapiens cysteine-rich repeat-containing protein S52 precursor, mRNA, complete cds Page 167 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORFSEQ Expression (Top) Hit Top Hit Acession Toph It SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E NO. NO: NO: Source Value 1770 6762 11850 3.83 1.0E-120 4557250 NT Homo sapiens disintegrn and metalloprotease domain 10 (ADAM10) mRNA 2049 7031 12143 1.03 1.0E-120 AB011399. 1 NT Homo sapiens gene for AF-6, complete cds 2049 7031 12144 1. 031. 0E-120 AB011399. 1 NT Homo sapiens gene for AF-6, complete cds 3235 5356 10368 0. 99 1. OE-120 4507334 NT Homo sapiens synaptojanin 1 (SYNJ1), mRNA 4232 9226 14209 1. 43 1. 0E-120 AF056490. 1 NT Homo sapiens cAMP-specific phosphodiesterase 8A (PDE8A) mRNA, partial cds 4232 9226 14210 1.43 1.0E-120 AF056490. 1 NT Homo sapiens cAMP-specific phosphodiesterase 8A (PDE8A) mRNA, partial cds 4527 9517 14503 2. 41 1. OE-120 AF098463. 1 NT Homo sapiens stanniocalcin (STC) gene, partial cds 4527 9517 14504 2. 41 1. OE-120 AF098463. 1 NT Homo sapiens stanniocatcin (STC) gene, partial cds 72 5150 10159 2. 65 1. OE-121 Y18000. 1 NT Homo sapiens NF2 gene 377 5424 10439 1. 27 1. OE-121 AU134963. 1 EST HUMAN AU134963 PLACE1 Homo sapiens cDNA clone PLACE1000899 5' 714 7728 10754 1.28 1.0E-121 5032192 NT Homo sapiens TNF receptor-associated factor 1 (TRAF1) mRNA 2501 7469 12584 1.28 1.0E-121 BF344378. 1 EST_HUMAN 602014759F1 NCI_CGAP_Brn64 Homo sapiens cDNA clone IMAGE : 4150286 5' 2501 7469 12585 1.28 1.0E-121 BF344378. 1 EST HUMAN 602014759F1 NCI_CGAP Brn64 Homo sapiens cDNA clone IMAGE : 4150286 5' 2899 7918 12939 0. 88 1. OE-121 AF111168. 2 NT Homo sapiens serine palmitoyl transferase, subunit II gene, complete cds ; and unknown genes 3007 8024 13036 4. 04 1. OE-121 Y19208. 1 NT Homo sapiens hHb3 gene for hair keratin, exons 1 to 9 3007 8024 13037 4. 04 1. OE-121 Y19208. 1 NT Homo sapiens hHb3 gene for hair keratin, exons 1 to 9 3459 8467 13494 0.84 1.0E-121 AB033758. 1 NT Homo sapiens mRNA for KIAA1337 protein, partial cds 3459 8467 13495 0.84 1.0E-121 AB037758. 1 NT Homo sapiens mRNA for KIAA1337 protein, partial cds 3593 8600 13607 8.01 1.0E-121 AF155156. 2 NT Homo sapiens adaptor-related protein complex AP-4 epsilon subunit mRNA, complete cds 4204 9197 14179 1.34 1.0E-121 AI263294. 1 EST HUMAN qx57b01. x1 NCI CGAP Pan1 Homo sapiens cDNA clone IMAGE : 2005417 3' 4817 9801 14781 3. 38 1. OE-121 X91937. 1 NT H. sapiens ECE-1 gene (exon 17) 5008 9979 14954 1. 26 1. OE-121 AB032481. 1 NT Homo sapiens HOXD13 gene for homeobox transcription factor, complete cds 2655324 10333 1. 82 1. OE-122 11526176 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1), mRNA 334 5386 10393 3. 2 1. OE-122 AF114488. 1 NT Homo sapiens intersectin short isoform (ITSN) mRNA, complete cds 356 5406 10418 1. 71 1. OE-122 11526176 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1), mRNA 871 5889 10931 2.93 1.0E-122 AF114488. 1 NT Homo sapiens intersectin short isoform (ITSN) mRNA, complete cds 1200 6201 11238 3. 95 1. OE-122 M20707. 1 NT Human kappa-immunoglobulin germline pseudogene (Chr22. 4) variable region (subgroup V kappa 11) 1654 6650 11722 3.55 1.0E-122 AF167706. 1 NT Homo sapiens cysteine-rich repeat-containing protein S52 precursor, mRNA, complete cds 1674 6670 11746 5.77 1.0E-122 11418424 NT Homo sapiens collagen, type XII, alpha 1 (COL12A1), mRNA 1674 6670 11747 5. 77 1. OE-122 11418424 NT Homo sapiens collagen, type XI, alpha 1 (COL12A1), mRNA 1778 6770 11862 4.64 1.0E-122 BE906024. 1 EST HUMAN 601497032F1 NIH MGC 70 Homo sapiens cDNA clone IMAGE : 3899358 5' 2420 7391 12511 20. 14 1. OE-122 BF316170. 1 EST HUMAN 601896173F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 4125234 5' 2420 7391 12512 20. 14 1.0E-122 BF316170.1 EST_HUMAN 601896173F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 4125234 5' Page 168 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor NO : NO : ID NO : Signal BLAST E No. source Valut Homo sapiens FYVE domain-containing dual specificity protein phosphatase FYVE-DSP2 mRNA, complete 2766 7787 12810 0. 98 1. OE-122 AF264717. 1 NT cds 4699 9684 14667 2. 44 1. 0E-122 4502166 NT Homo sapiens amySoid beta (A4) precursor protein (protease nexini-II, Alzheimer disease) (APP), mRNA 4832 9816 1.49 1.0E-122 AW504645, 1 EST_HUMAN UI-HF-BN0-all-a-03-0-Ul.r1 NIH_MGC_50 Homo sapiens cDNA clone IMAGE : 3079948 5' 759 5780 10807 2. 05 1. OE-123 BF345274. 1 EST_HUMAN 602018058F1 NCI_CGAP_Brn67 Homo sapiens cDNA clone IMAGE : 4153670 5' 759 5780 10808 2. 05 1. OE-123 BF345274. 1 EST HUMAN 602018058F1 NCI CGAP Brn67 Homo sapiens cDNA clone IMAGE : 4153670 5' 997 6009 11039 5.43 1. OE-123 AL163249. 2 NT Homo sapiens chromosome 21 segment HS21 C049 1006 6016 11046 6. 48 1. OE-123 5803114 NT Homo sapiens inner membrane protein, mitochondrial (mitofilin) (IMMT), mRNA Homo sapiens phosphatidylinostiol-4-phosphate 5-kinase, type 11, beta (PIP5K2B) mRNA, and translated 1219 6218 11260 4. 18 1. OE-123 4505818 NT products Homo sapiens phosphatidylinositol-4-phosphate 5-kinase, type 11, beta (PIP5K2B) mRNA, and translated 1219 6218 111261 4.18 1.0E-123 4505818 NT products 1428 6425 11484 2.56 1.0E-123 AJ388641.1 NT Homo sapines partial mRNA for immunoglobulin kappa chain variable region (IGVK gene), sampel GN02 2042 7024 12134 1. 75 1.0E-123 M55419. 1 NT Human amelogenin (AMELY) gene, 3'end of cds 2042 7024 12135 1. 75 1. 0E-123 M55419. 1 NT Human amelogenin (AMELY) gene, 3'end of cds 2042 7024 12136 1.75 1.0E-123 M55419.1 NT Human amelogenin (AMELY) gene, 3'end of cds 2251 7228 2. 82 1. 0E-123 7705962 NT Homo sapiens RAB9-like protein (LOC51209), mRNA 4326 9318 0. 93 1. OE-123 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21C080 266 5325 10334 2. 41 1. OE-124 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA 266 5325 10335 2. 41 1. 0E-124 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA 272 5331 1. 29 1. OE-124 D87675. 1 NT Homo sapiens DNA for amyfoid precursor protein, complete cds 482 5519 10530 2. 33 1. OE-124 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 zt81 b04. r1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE : 728719 5' similar to TR : G300482 682 5706 10717 5. 13 1. OE-124 AA397551. 1 EST HUMAN G300482 POL=REVERSE TRANSCRIPTASE HOMOLOG {RETROVIRAL ELEMENT} zt81b04. r1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE : 728719 5' similar to TR : G300482 682 5706 10718 5.13 1.0E-124 AA397551.1 EST_HUMAN G300482 POL=REVERSE TRANSCRIPTASE HOMOLOG (RETROVIRAL ELEMENT) ; 749 5771 10798 8. 09 1. OE-124 AF155654. 1 NT Human putative ribosomal protein S1 mRNA 799 5820 10850 1. 42 1. OE-124 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA 893 5911 10950 2. 96 1. OE-124 7705446 NT Homo sapiens hypothetical protein (HSPC068), mRNA 1328 6326 11373 4. 52 1. OE-124 AF274892. 1 NT Homo sapiens glucose transporter 3 gene, exons 9, 10, and complete cds 1328 6326 11374 4. 52 1. OE-124 AF274892. 1 NT Homo sapiens glucose transporter 3 gene, exons 9, 10, and complete cds 1779 6771 11863 4,44 1.0E-124 AJ131712.1 NT Homo sapines mRNA for nucleolar RNA-hellcase (noH61 gene) - Page 169 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession pabase Top Hit Descriptor NO : NO : ID NO : Signal BLAST E No. Source, Value Value 2009 6992 12096 1. 32 1. OE-124 BE879524. 1 EST HUMAN 601491715F1 NIH_MGC 69 Homo sapiens cDNA clone IMAGE : 3893954 5' 2388 7359 12481 1. 32 1. OE-124 AB024069. 1 NT Homo sapiens gene for B120, exon 11 3410 8419 13447 0. 74 1. OE-124 S78684. 1 NT Homo sapiens ATP-sensitive inwardly rectifying K-channel subunit (KCNJ6IBIR1) gene, exon 3410 8419 13448 0. 74 1. OE-124 S78684. 1 NT Homo sapiens ATP-sensitive inwardly rectifying K-channel subunit (KCNJ6IBIR1) gene, exon 3564 8571 13577 1. 19 1. OE-124 X13794. 1 NT H. sapiens lactate dehydrogenase B gene exon 1 and 2 (EC 1. 1. 1. 27) (and joined CDS) 3804 8807 13813 0. 88 1. OE-124 4507500 NT Homo sapiens T-ceii lymphoma invasion and metastasis 1 (TIAM1) mRNA 3962 8960 13950 0. 81 1. OE-124 4504116 NT Homo sapiens glutamate receptor, ionotopic, kainate 1 (GRIK1) mRNA 4601 9589 14576 2. 01 1. OE-124 AB024069. 1 NT Homo sapiens gene for 8120, exon 11 4778 9762 1. 39 1. OE-124 M18178. 1 NT Human fibronec6n gene extra type III repeat (EDII), exon x+1 317 5372 11. 96 1. OE-125 AB032998. 1 NT Homo sapiens mRNA for KIAA1172 protein, partial cds 423 5081 10065 4. 13 1. OE-125 BE743922. 1 EST HUMAN 601577981F1 NIH_MGC 9 Homo sapiens cDNA clone IMAGE : 3926685 5' 718 5741 10758 1. 95 1. OE-125 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds zk53c07. s1 Soaresregnant uterus NbHPU Homo sapiens cDNA clone IMAGE : 486540 3'similar to 849 5868 10909 3. 29 1. OE-125 AA042813. 9 EST HUMAN gb : X65857 cds1 OLFACTORY RECEPTOR-LIKE PROTEIN HGMP07E (HUMAN) ; 983 5998 11029 1. 63 1. OE-125 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21 C010 1136 6141 11170 1. 77 1. OE-125 7662279 NT Homo sapiens KIAA0744 gene praluct ; histone deacetylase 7 (KIAA0744), mRNA 1775 6767 11858 2. 36 1. OE-125 AF015450. 1 NT Homo sapiens Usurpin-alpha mRNA, complete cds 1775 6767 11859 2. 36 1. OE-125 AF015450. 1 NT Homo sapiens Usurpin-alpha mRNA, complete cds 22g7 7272 12389 2. 15 1. OE-125 AA011278. 1 EST HUMAN zi01g09. r1 Soares fetal liver spleen_1NFLS S1 Homo sapiens cDNA clone IMAGE : 429568 5' zk53c07. s1 Soaresregnant uterus NbHPU Homo sapiens cDNA clone IMAGE : 486540 3'similar to 2431 7402 12521 1. 54 1. OE-125 AA042813. 1 EST HUMAN gb : X65857 cdsl OLFACTORY RECEPTOR-L (KE PROTEIN HGMP07E (HUMAN) ; 2520 7487 12605 1. 74 1. OE-125 4504696 NT Homo sapiens inhibin, alpha (INHA) mRNA 2520 7487 12fi06 1. 74 1. OE-125 4504696 NT Homo sapiens inhibin, alpha (INHA) mRNA bb74f06. y1 NIH MGC 12 Homo sapiens cDNA clone IMAGE : 3048131 5'similar to TR : 095604 095604 2940 10044 12978 0. 98 1. oE-125 BE018009. 1 EST HUMAN ZINC FINGER PROTEIN. ; K zk53c07. s1 Soaresregnant uterus NbHPU Homo sapiens cDNA clone IMAGE : 486540 3'simila to 3768 8771 13776 2. 21 1. OE-125 AA042813. 1 EST HUMAN gb : X65857 cds1 OLFACTORY RECEPTOR-LIKE PROTEIN HGMP07E (HUMAN) ; 4419 9409 14396 2. 44 1. OE-125 11425114 NT Homo sapiens zinc finger protein ZNF287 (ZNF287), mRNA 4419 9409 14397 2. 44 1. OE-125 11425114 NT Homo sapiens zinc finger protein ZNF287 (ZNF287), mRNA 4484 9474 14454 1. 81 1. OE-125 BE315412. 1 EST HUMAN 601141152F1 NIH MGC 9 Homo sapiens cDNA clone IMAGE : 3140796 5' 765 5786 10815 1. 81 1. OE-126 4758007 NT Homo sapiens CDC-like kinase (CLK) mRNA 768 5789 10818 2. 59 1. OE-126 M61936. 1 NT Human laminin B1 chain gene, exon 20 908 5925 10960 1. 36 1. OE-126 X68735. 1 NT H. sapiens gene for alpha1-antichymotrypsin, exon 3 2283 7259 12377 1. 21 1. OE-126 8923056 NT Homo sapiens hypothetical protein FLJ20048 (FLJ20048), mRNA Page 170 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acessino Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor Value 2283 7259 12378 1. 21 1.0E-126 8923056 NT Homo sapiens hypothetical protein FLJ20048 (FLJ20048), mRNA 2521 7488 12607 2. 83 1. OE-126 6382078 NT Homo sapiens RAN binding protein 2 (RANBP2), mRNA 2999 8017 13030 54. 41 1. OE-126 AA160709. 1 EST HUMAN zo72c03. r1 Stratagene pancreas (#937208) Homo sapiens cDNA clone IMAGE : 592420 5' 2999 8017 13031 54. 41 1. OE-126 AA160709. 1 EST_HUMAN zo72c03.r1 Stratagene 4type XI alpha-1 (COL11A1) pancreas (#937208) Homo sapiens cDNA clone IMAGE : 592420 5' 3545 8552 13559 0. 75 1. OE-126 X53941. 1 NT H. sapiens DNA for liver cytochrome b5 pseudogene 3571 8578 13584 2. 041. 0E-1267657038 NT Homo sapiens death receptor 6 (DR6), mRNA 4642 9627 14619 0. 98 1. OE-126 AF101108. 1 NT Homo sapiens collagen 5236 10247 8.4 1.0E-127 AB024597. gene, exon 63 4642 9627 14620 0. 98 1. OE-126 AF101108. 1 NT Homo sapiens collagen type X (alpha-1 (COL11A1) gene, exon 63 4687 9672 14654 1. 53 1. OE-126 N34078. 1 EST HUMAN yx78c06. r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 267850 5' 171 5236 10247 8. 83 1. OE-127 AB024597. 1 NT Homo sapiens mRNA for casein kinase I epsilon, complete cds 171 5236 10248 8. 83 1. OE-127 AB024597. 1 NT Homo sapiens mRNA for casein kinase I epsilon, complete cds 172 45 1.0E-127 1 NT Homo sapiens mRNA for casein kinase I epsilon, complete cds 172 5236 10248 8. 4 1. 0-127 AB024597. 1 NT Homo sapiens mRNA for casein kinase I epsilon, complete cds 271 5330 10342 1. 45 1. OE-127 D87675. 1 NT Homo sapiens DNA for amytoid precursor protein, complete cds 271 5330 10343 1. 36 1.0E-127 AF114488. D87675. 1 NT Homo sapiens DNA for amyioid precursor protein, complete cds 870 5888 10930 1. ubiquitin 1 NT Homo sapiens intersectin short isoform (ITSN) mRNA, complete cds 905 5922 10956 1. 51 1. OE-127 U72621. 2 NT Homo sapiens lost on transformation LOT1 mRNA, complete cds 1652 6648 11720 1. 92 1. OE-127 4827053 NT Homo sapiens (LILRA1), specific protease 8 (USP8) mRNA Homo sapiens leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1 09 2013 6996 12099 2. 1.0E-127 5803065 NT mRNA Homo sapiens leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 1 (LILRA1), 2013 6996 12100 2. 09 1.0E-127 5803065 NT mRNA 2137 7117 12231 38. 06 1. OE-127 4506620 NT Homo sapiens ribosomal protein L26 (RPL26) mRNA 2280 7256 12374 2. 58 1. OE-127 AF245505. 1 NT Homo sapiens adlican mRNA, complete cds 2535 7500 12620 16. 49 1. OE-127 X12881. 1 NT Human mRNA for cytokeratin 18 3606 8613 13621 0. 82 1.0E-127 AF114488. 1 NT Homo sapiens intersectin short isoform (ITSN) mRNA, complete cds au80e06. y-Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2782594 5'similar to TR : Q15170 Q15170 TRANSCRIPTION FACTOR S-it-RELATED PROTEIN ; contains element MER22 3721 8725 13725 17.44 1.0E-127 AW161297. 1 EST HUMAN repetitive element ; 4132 9127 14110 22. 49 1. OE-127 7706239 NT Homo sapiens neuroblastoma-amplified protein (LOC51594), mRNA 4132 9127 14111 22. 49 1. OE-127 7706239 NT Homo sapiens neuroblastoma-amplified protein (LOC51594), mRNA 472 9462 14441 5.1 1.0E-127 4506384 NT Homo sapiens RAD1 (S. pombe) homolog (RAD1) mRNA, and translated products 4498 9488 3.63 1.0E-127 AL163268. 2 NT Homo sapiens chromosome 21 segment HS21C068 4537 9527 14513 1.21 1.0E-127 6912639 NT Homo sapiens Ring1 and YY1 binding protein (RYBP), mRNA Page 171 of 209 Table 4 Sinale Exon Probes Exoressed in HBL100 Cells Most Similar Top Hit Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databse Top Hit Descriptor ID NO: Signal RI ASTE No NO : NO @ ID NO: Signal BLAST E No. Source Value 457 5494 10506 4. 57 1. OE-128 BE385617. 1 EST HUMAN 601278127F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3618822 5' 2018 7001 12104 12. 06 1. OE-128 U02523. 1 NT Human FAU1P pseudogene, trinucleotide repeat regions 2018 7001 12105 12. 06 1. OE-128 U02523. 1 NT Human FAU1P pseudogene, trinucleotide repeat regions 2147 7126 12243 127. 93 1. 0E-128 4506718 NT Homo sapiens ribosomal protein S2 (RPS2) mRNA 2377 7349 4. 72 1. OE-128 11437455 NT Homo sapiens chromatin-specific transcription elongation factor, 140 kDa subunit (FACTP140), mRNA 3310 8321 13344 1. 28 1. OE-128 AB033073. 1 NT Homo sapiens mRNA for KIAA1247 protein, partial cds 4529 9519 14506 4. 83 1. 0E-128 11426673 NT Homo sapiens prospero-related homeobox 1 (PROX1), mRNA 122 5447 10469 1. 18 1. 0E-129 S37722. 1 NT insulin-like growth factor binding protein-2 [human, placenta, Genomic, 1019 nt, segment 2 of 4] 410 5447 10469 1. 25 1. OE-129 S37722. 1 NT insulin-like growth factor binding proteini-2 [human, placenta, Genomic, 1019 nt, segment 2 of 4] 1680 6676 11750 3. 33 1. OE-129 AL096880. 1 NT Novel human mRNA containing Zinc finger C2H2 type domains Homo sapiens glutathione S-transferase theta 2 (GSTT2) and glutathione S-transferase theta 1 (GSTT1) 1685 6681 11755 1. 56 1. OE-129 AF240786. 1 NT genes, complete cds Homo sapiens glutathione S-transferase theta 2 (GSTT2) and glutathione S-transferase theta 1 (GSTT1) 1685 6681 11756 1. 56 1. OE-129 AF240786. 1 NT genes, complete cds 1809 6799 11890 5. 19 1. OE-129 11418522 NT Homo sapiens zinc finger protein 76 (expressed in testis) (ZNF76), mRNA 3055 8072 13082 1. 71 1. 0E-129 Q14585 SWISSPROT ZINC FINGER PROTEIN HZF10 3055 8072 13083 1. 71 1. 0E-129 Q14585 SWISSPROT ZINC FINGER PROTEIN HZF10 3055 8072 13084 1. 71 1. 0E-129 Q14585 SWISSPROT ZINC FINGER PROTEIN HZF10 4010 9006 13994 1. 03 1. OE-129 5032230 NT Homo sapiens A kinase (PRKA) anchor protein 9 (AKAP9), mRNA 4010 9006 13995 1. 03 1. OE-129 5032230 NT Homo sapiens A kinase (PRKA) anchor protein 9 (AKAP9), mRNA 4040 9036 14026 1. 96 1. OE-129 AB040892. 1 NT Homo sapiens mRNA for KIAA1459 protein, partial cds CMYA5 Human cardiac muscle expression library Homo sapiens cDNA clone 4151935 similar to CMYA5 4158 9153 14135 2. 16 1. 0E-129 AW755254. 1 EST_HUMAN Cardiomy6opathy associated gene 5 CMYA5 Human cardiac muscle expression library Homo sapiens cDNA clone 4151935 similar to CMYA5 4158 9153 14136 2. 16 1. OE-129 AW755254. 1 ESTHUMAN Cardiomyopathy associated gene 5 75 5153 10163 1. 95 1. 0E-130 7705530 NT Homo sapiens hypothetical protein (HSPC242), mRNA 1627 6624 11693 31. 59 1. OE-130 BE275192. 1 EST HUMAN 601121995F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3346366 5' 1627 6624 11694 31. 59 1. 0E-130 BE275192. 1 EST HUMAN 601121995F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3346366 5' 1937 6923 2. 05 1. OE-130 X04092. 1 NT Human gene for catalase (EC 1. 11. 1. 6) exon 9 mapping to chromosome 11, band p13 2698 7655 5.31 1.0E-130 AJ010230.1 NT Homo sapiens RET finger protein-like 1 antisense transcript, partial 2809 7829 12844 1.17 1.0E-130 BE564219. 1 EST_HUMAN 601343016F1 NIH_MGC 53 Homo sapiens cDNA clone IMAGE : 3685466 5' 2809 7829 12845 1. 17 1.0E-130 BE564219. 1 EST_HUMAN 601343016F1 NIH_MGC_53 Homo sapiens cDNA clone IMAGE : 3685466 5' 3497 8505 13520 0.78 1.0E-130 AF240698. 1 NT Homo sapiens retina dehydrogenase homolog isoform-1 (RDH) mRNA, complete cds Page 172 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORFSEQ Expression (Top) Hit Top Hit Acessoin Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Value 3681 7829 12844 5. 51 1. OE-130 BE564219. 1 EST_HUMAN 601343016F1 NIH_MGC_53 Homo sapiens cDNA clone IMAGE : 3685466 5' 3681 7829 12845 5.51 1.0E-130 BE564219. 1 EST-HUMAN 601343016F1 NIH_MGC-53 Homo sapiens cDNA clone IMAGE : 3685466 5' 3833 8835 13841 1. 54 1. OE-130 AW503580. 1 EST HUMAN Ul-HF-BNO-aky-g-06-0-Ul. r1 NIH MGC 50 Homo sapiens cDNA clone IMAGE : 3078731 5' 3960 8958 13947 1. 251. 0E-130 M97710. 1 NT Human T-cell receptor (V alpah 22. 1, J alpha RPMI4265-variant, C alpha 1) mRNA 4408 9399 14383 9. 21 1. 0E-130 AW843993. 1 EST HUMAN CM4-CN0045-180200-511-f02 CN0045 Homo sapiens cDNA 4926 9903 14880 1. 3 1. OE-130 AW363299. 1 EST_HUMAN RC0-CT0318-201199-031-a11 CT0318 Homo sapiens cDNA 4926 9903 14881 1. 3 1. OE-130 AW363299. 1 EST_HUMAN RC0-CT0318-201199-031-a11 CT0318 Homo sapiens cDNA zr58c04. r1 soares_NhHMPu-S1 Homo sapiens cDNA clone IMAGE : 667590 5'similar to TR : G222811 4 5085 10069 1.8 0.0E+00 AA228126.1 EST_HUMAN G222811 ALPHA 1 CHAIN OF TYPE XII COLLAGEN. ; zr58c04.r1 Soares_NhHMPU_S1 Homo sapiens cDNA clone IMAGE:667590 5' similar to TR;G222811 4 5085 10070 1.8 0. OE+00 AA228126. 1 EST HUMAIN G222811 ALPHA 1 CHAIN OF TYPE XII COLLAGEN. ; 8 5088 10074 1. 14 O. OE+00 4885136 NT Homo sapiens checkpoint suppressor 1 (CHES1), mRNA 21 5101 10085 5. 04 O. OE+00 D83327. 1 NT Homo sapiens DCRR1 mRNA, partial cds 21 5101 10086 5. 04 O. OE+00 D83327. 1 NT Homo sapiens DCRR1 mRNA, partial cds 27 5107 10091 107. 34 O. OE+00 AF141349. 1 NT Homo sapiens beta-tubulin mRNA, complete cds 35 5115 10101 1. 77 O. OE+00 5802997 NT Homo sapiens Cdc42 effector protein 2 (CEP2), mRNA 37 5117 10104 0. 74 O. OE+00 M58600. 1 NT Human heparin cofactor II (HCF2) gene, exons 1 through 5 41 5121 10109 24. 17 O. OE+00 6857825 NT Homo sapiens RNA-binding protein S1, serine-rich domain (RNPS1), mRNA 58 5138 10136 4. 3 O. OE+00 Y17151. 2 NT Homo sapiens mRNA for mulddrug resistance protein 3 (ABCC3) 58 5138 10137 4. 3 O. OE+00 Y17151. 2 NT Homo sapiens mRNA for multidrug resistance protein 3 (ABCC3) 60 5140 10141 2.89 0.0E+00 D78804. 1 ESTHUMAN HUM516H08B Human placenta poly-A+ (TFujiwara) Homo sapiens cDNA clone GEN-516H08 5' 60 5140 10142 2. 89 O. OE+00 D78804. 1 EST HUMAN HUM516H08B Human placenta polyA+(TFujiwara) Homo sapiens cDNA clone GEN-516H08 5' 61 5141 10143 54. 73 O. OE+00 L16558. 1 NT Human ribosomal protein L7 (RPL7) mRNA, complete cds 63 5143 10146 11. 31 O. OE+00 AW069534. 1 ESTHUMAN cr48e07. x1 Jia bone marrow stroma Homo sapiens cDNA clone HBMSC cr48e07 3' 63 5143 10147 11.31 0.0E+00 AW069534.1 EST_HUMAN cr48e07.x1 Jia bone marrow stroma Homo sapiens cDNA clone HBMSCcr48e07 3' 74 5152 10161 2. 95 O. OE+00 4758977 NT Homo sapiens protein tyrosine phosphatase, non-receptor type substrate 1 (PTPNS1) mRNA 74 5152 10162 2.95 0.0E+00 475977 NT Homo sapiens protein tyrosine phosphatase, non-receptor type substrate 1 (PTPNS1) mRNA 77 5152 10161 1.73 0.0E+00 4758977 NT Homo sapiens protein tyrosine phosphatase, non-receptor type substrate 1 (PTPNS1) mRNA 77 5152 10162 1. 73 O. OE+00 4758977 NT Homo sapiens protein tyrosine phosphatase, non-receptor type substrate 1 (PTPNS1) mRNA Homo sapiens amiloride binding protein 1 (amine oxidase (copper-containing)) (ABP1), nuclear gene 80 5157 10168 0. 7 0.0E+00 4501850 NT encoding mitochondrial protein, mRNA 81 5158 50. 8 O. OE+00 4504444 NT Homo sapiens heterogeneous nuclear ribonucleoprotein A1 (HNRPA1) mRNA 90 5167 1077 77. 93 O. OE+00 5016088 NT Homo sapiens actin, beta (ACTB) mRNA 93 5170 10180 45. 78 0.0E+00 U89277.1 NT Human polyhomeotic 1 homolog (HPH1) mRNA, partial cds Page 173 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Eression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No. Database Top Hit Descriptor NO : NO : Source, Value 100 5177 10187 2. 36 O. OE+00 AI114743. 1 EST HUMAN HA1347 Human fetal liver cDNA library Homo sapiens cDNA 101 5178 10188 1. 89 O. OE+00 A8037784. 1 NT Homo sapiens mRNA for KIAA1363 protein, partial cds ts38b05. x1 NCI CGAP Ut4 Homo sapiens cDNA clone IMAGE : 2230833 3'similar to TR : Q99551 Q99551 116 5188 10197 0. 91 O. OE+00 AI623701. 91 EST HUMAN MITOCHONDRIAL TRANSCRIPTION TERMINATION FACTOR PRECURSOR. MITOCHONDRIAL ts38b05. x1 NCI CGAP Ut4 Homo sapiens cDNA clone IMAGE : 2230833 3'similar to TR : Q99551 Q99551 117 5188 10197 1. 65 O. OE+00 AI623701. 1 EST HUMAN MITOCHONDRIAL TRANSCRiPTION TERMINATfON FACTOR PRECURSOR. ; 118 7690 10198 3. 4 O. OE+00 N36040. 1 EST HUMAN yy01 h09. r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 270017 5' 118 7690 10199 3. 4 O. OE+00 N36040. 1 EST HUMAN yy01h09. r1 Soares melanocyte 2NbHM Homo sapiens cDNA clone IMAGE : 270017 5' 121 5191 10204 0. 7 O. OE+00 4505458 NT Homo sapiens neuropilin 2 (NRP2) mRNA 131 5197 10212 4. 8 O. OE+00 4505938 NT Homo sapiens polymerase (RNA) II (DNA directed) polypeptide A (220kD) (POLR2A) mRNA 131 5197 10213 4. 8 O. OE+00 4505938 NT Homo sapiens polymerase (RNA) II (DNA directed) polypeptide A (220kD) (POLR2A) mRNA 140 5205 10220 1. 18 O. OE+00 T56945. 1 EST_HUMAN Yes3904. r2 Stratagene fetal spleen (#1937205) Homo sapiens cDNA clone IMAGE : 68310 5' 140 5205 10221 1. 18 O. OE+00 T56945. 1 EST HUMAN ya94. r2 Stratagene fetal spleen (#l937205) Homo sapiens cDNA clone IMAGE : 68310 5' 152 5218 70. 7 O. OE+00 4504444 NT Homo sapiens heterogeneous nuclear ribonucleoprotein A1 (HNRPA1) mRNA 156 5222 10234 3. 22 O. OE+00 BF036881. 1 EST HUMAN 601460375F1 NIH MGC 66 Homo sapiens cDNA cione IMAGE : 3863803 5' 158 5224 143. 39 O. OE+00 4504444 NT Homo sapiens heterogeneous nuclear ribonucleaprotein A1 (HNRPA1) mRNA 161 5227 10237 0. 82 O. OE+00 AF111168. 2 NT Homo sapiens serine palmitoyl transferase, subunit II gene, complete cds ; and unknown genes 163 5229 10238 6. 28 O. OE+00 BE295973. 1 EST HUMAN 601174270F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 3529864 5' 164 5229 10238 1. 45 O. OE+00 BE295973. 1 EST HUMAN 601174270F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 3529864 5' zd62b05. r1 Soares fetal heart NbHH19W Homo sapiens cDNA clone IMAGE : 345201 5'similar to _ 165 5230 10239 6. 65 O. OE+00 W73973. 1 EST_HUMAN gb : X16282 cds1 ZINC FINGER PROTEIN CLONE 647 (HUMAN) ; 166 5231 10240 2. 09 O. OE+00 BE962832. 1 EST_HUMAN QV3-HT0457-140200-088-d04 HT0457 Homo sapiens cDNA 166 5231 10241 2. 09 O. oE+00 BE162832. 1 EST HUMAN QV3-HT0457-140200-088-d04 HT0457 Homo sapiens cDNA 167 5232 10242 3. 77 O. OE+00 AF244088. 1 NT Homo sapiens zinc finger protein mRNA, complete cds 170 5235 10245 15. 16 O. OE+00 AL163202. 2 NT Homo sapiens chromosome 21 segment HS21C002 170 5235 10246 15. 16 O. OE+00 AL163202. 2 NT Homo sapiens chromosome 21 segment HS21 C002 bb24e12. y1 NIH_MGC 14 Homo sapiens cDNA clone IMAGE : 2963854 5'similar to W P : Y57A10A. Z 180 5243 10253 9. 67 O. OE+00 BE018970. 1 EST HUMAN CE22631 ; bb24e12. y1 NIH_MGC 14 Homo sapiens cDNA clone IMAGE : 2963854 5'similar to W P : Y57A10A. Z 180 5243 10254 9. 67 O. OE+00 BE018970. 1 EST HUMAN CE22631 ; 185 5248 10257 4. 23 O. OE+00 AB018327. 1 NT ^ Homo sapiens mRNA fa KIAA0784 protein, partial cds 185 5248 10258 4. 23 O. OE+00 AB018327. 1 NT Homo sapiens mRNA fa KIAA0784 protein, partial cds 186 5249 10259 3. 83 O. OE+00 AB018327. 1 NT Homo sapiens mRNA fa KIAA0784 protein, partial cds 186 5249 10260 3. 83 O. OE+00 AB018327. 1 NT Homo sapiens mRtJA fx KIAA0784 protein, partial cds _ Page 174 of 209 Table 4 Sinale Exon Probes Exoressed in HBL100 Cells Probe exon Most Similar Probe SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Top Hit Descriptor NO: NO: Value Source 194 5258 10271 261.78 0.0E+00 D50659. 1 NT Human gamma-cytoplasmic actn (ACTGP9) pseudogene 199 S263 102763. 01O. OE+00 AF273045. 1 NT Homo sapiens CTCL tumor antigen se14-3 mRNA, complete cds 199 5263 10277 3. 01 0.0E+00 AF273045. 1 NT Homo sapiens CTCL tumor antigen se14-3 mRNA, complete cds 201 5265 10279 28. 3 0.0E+00 AF167174. 1 NT Homo sapiens chromosome X MSL3-2 protein mRNA, complete cds 201 5265 10280 28. 3 O. 0E+00 AF167174. 1 NT Homo sapiens chromosome X MSL3-2 protein mRNA, complete cds tq04f08. x1 NCI_CGAP_Ut3 Homo sapiens cDNA clone IMAGE : 2207847 3'similar to gb : J03191 PROFILIN I 210 7715 10286 57. 42 0.0E+00 ai587308. 1 EST HUMAN (HUMAN) ; tp04f08.x1 NCI_CGAP-Ut3 Homo sapiens cDNA clone IMAGE : 2207847 3'similar to gb : J03191 PROFILIN I 210 7715 10287 57. 42 0.0E+00AI587308. 1 EST HUMAN (HUMAN) ; 212 5275 10289 1. 9 0.0E+00 AF195658. 1 NT Homo sapiens DNA mismatch repair protein (MLH3) gene, complete cds 214 5277 99. 79 O. OE+00 4506632 NT Homo sapiens ribosomal protein L31 (RPL31) mRNA 215 5278 15. 17 O. OE+00 AF132000. 1 NT Homo sapiens TADA1 protein mRNA, complete cds 220 5283 10293 3. 41 O. OE+00 AB018264. 1 NT Homo sapiens mRNA for KIAA0721 protein, partial cds 221 5283 10293 3.35 0.0E+00 AB018264. 1 NT Homo sapiens mRNA for KIAA0721 protein, partial cds 222 5284 10294 4. 92 0.0E+00 6678444 NT Mus musculus testis-specific protein, Y-encoed-like (Tspy), mRNA 236 5298 10309 16. 43 O. OE+00 5453805 NT Homo sapiens NS1-associated protein 1 (NSAP1) mRNA 238 5300 17. 29 O. OE+00 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21C001 245 5305 10315 3.82 0.0E+00 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 247 5307 10318 1. 5 0.0E+00 X89772. 1 NT H. sapiens mRNA for interferon alpha/beta receptor (long form) 255 5315 6.35 0.0E+00 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 267 5326 10336 1. 48 O. OE+00 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 267 5326 10337 1. 48 O. OE+00 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA 269 5328 10339 2. 14 O. OE+00 7706028 NT Homo sapiens hypothetical protein (LOC51250), mRNA 280 5338 2. 81 O. OE+00 D83327. 1 NT Homo sapiens DCRR1 mRNA, partial cds 281 5339 10352 2. 43 O. OE+00 D83327. 1 NT Homo sapiens DCRR1 mRNA, partial cds 281 5339 10353 2.43 0.0E+00 D83327. 1 NT Homo sapiens DCRR1 mRNA, partial cds 282 5340 0.88 0.0E+00 AW845293. 1 EST_HUMAN IL-2CT0031-181199-020-B03 CT0031 Homo sapiens cDNA 291 5348 10360 5. 4 0.0E+00 4557029 NT Homo sapiens potassium inwardly-rectifying channel, subfamily member 15 (KCNJ15) mRNA 291 5348 10361 5.4 0.0E+00 4557029 NT Homo sapiens potassium inwardly-rectifying channel, subfamily J, member 15 (KCNJ15) mRNA 302 5359 10371 5.25 0.0E+00 AB028942.1 NT Homo sapiens mRNA for KIAA1019 protein, partial cds 303 5360 10372 4. 98O. OE+00 AB028942. 1NTHomo sapiens mRNA for KIAA1019 protein, partial cds 304 7718 46. 48 O. OE+00 4506728 NT Homo sapiens ribosomal protein S5 (RPS5) mRNA Homo sapiens phosphoribosyiglycinamide formyitransferase, phosphoribosyiglycinamide synthetase, 305 5361 10373 2. 02 0.0E+00 4503914 NT phosphoribosylaminoimidazoel synthetase (GART) mRNA Page 175 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor Value Source 306 5362 2. 32 O. OE+00 AA480002. 1 EST_HUMAN zv18c06.r1 Soares_NhHMPU_S1 Homo sapiens cDNA clone IMAGE:753994 5 307"536310374"20. 77O. OE+M4507152 NT Homo sapiens SON DNA binding protein (SON) mRNA 308 5363 10374 22. 16 0.0E+00 4507152 NT Homo sapiens SON DNA binding protein (SON) mRNA 312 5367 10378 2. 2 O. OE+00 AF114488. 1 NT Homo sapiens intersectin short isoform (ITSN) mRNA, complete cds 325 5379 10387 2. 91 O. OE+00 014867 SWISSPROT TRANSCRIPTION REGULATOR PROTEIN BACH1 (BTB AND CNC HOMOLOG 1) (HA2303) 325 5379 10388 2. 91 O. OE+00 014867 SWISSPROT TRANSCRIPTION REGULATOR PROTEIN BACH1 (BTB AND CNC HOMOLOG 1) (HA2303) 326 5380 10389 3. 75 O. OE+00 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 327 538010389 2. 04 0.0E+00 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog) ; translocated to, 4 342 5394 10401 2. 33 O. OE+00 5174574 NT (MLLT4) mRNA 343 5395 10402 1. 6 O. OE+00 4505256 NT Homo sapiens moesin (MSN), mRNA 346 5398 10406 10. 77 O. OE+00 4827057 NT Homo sapiens X-box binding protein 1 (XBP1) mRNA 349 5401 10411 2.29 0.0E+00 U71600.1 NT Human zinc finger protein zfp31 (zf31) mRNA, partial cds 354 5405 10415 2.65 0.0E+00 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 354 5405 10416 2.65 0.0E+00 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 355 7719 10417 3. 33 O. OE+00 AF231919. 1 NT Homo sapiens chromosome 21 unknown mRNA 357 5407 10419 1. 13 O. OE+00 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) mRNA 360 5410 10423 1. 36 O. OE+00 4503854 NT Homo sapiens GA-binding protein transcription factor, alpha subunit (60kD) (GABPA), mRNA 361 5411 10424 2. 25 O. OE+00 D80006. 1 NT Human mRNA for KIAA0184 gene, partial cds 362 5411 10424 1. 75 O. OE+00 D80006. 1 NT Human mRNA for KIAA0184 gene, partial cds 364 5413 10426 1.21 0.0E+00 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (TIAM1) MRNA 375 5422 10437 2.21 0.0E+00 AU134963.1 EST-HUMAN AU134963 PLACE1 Homo sapiens cDNA clone PLACE1000899 5' 386 54611 10479 7.9 0.0E+00 AB028942. 1 NT Homo sapiens mRNA for KIAA1019 protein, partial cds qy61h05.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE : 2018457 3'similar to gb : X54199 387 5462 10480 7. 2 0.0E+00 AI363014. 1 EST HUMAN PHOSPHORIBOSYLAMINE-GLYCINE LIGASE (HUMAN) ; 391 5429 10444 3.05 0.0E+00 AW754180. 1 EST_HUMAN RC2-CT0320-300100-016-a09 CT0320 Homo sapiens cDNA 394 5431 10447 1. 12 O. OE+00 4503680 NT Homo sapiens IgG Fc binding protein (FC (GAMMA) BP) mRNA 395 5432 10448 2. 02 O. OE+00 4503680 NT Homo sapiens IgG Fc binding protein (FC (GAMMA) BP) mRNA 395 5432 10449 2. 02 O. OE+00 4503680 NT Homo sapiens IgG Fc binding protein (FC (GAMMA) BP) mRNA 396 5433 10450 1. 2 O. OE+00 4503680 NT Homosa 397 5434 10451 1. 19 O. OE+00 4503680 NT Homo sapiens IgG Fc binding protein (FC (GAMMA) BP) mRNA 397 5434 10452 1. 19 O. OE+00 4503680 NT Homo sapiens is 398 5435 10453 3. 59 O. OE+00 4503680 NT Homo sapiens IgG Fc binding protein (FC (GAMMA) BP) mRNA 399 5436 10454 3.82 0.0E+00 x74870. 1 NT H. sapiens gene for RNA pol II largest subunit, exons 23-29 Page 176 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Nit Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databse Top Hit Descriptor ID NO: Signal RI ASTE No NO : NO: ID NO: Signal BLAST E No. source Value 399 5436 10455 3.82 0.0E+00 X74870.1 NT H.sapiens gene for RNA pol II largest subunit,e xons 23-29 400 5436 10454 3. 87 O. OE+00 X74870. 1 NT H. sapiens gene for RNA pol ll largest subunit, exons 23-29 400 5439 10455 3.87 0.0E+00 X74870.1 NT H. sapiens gene for RNA pol Il largest subunit, exons 23-29 404 5440 114. 34 0.0E+00 4506608 NT Homo sapiens ribosomal protein L19 (RPL19) mRNA 417 5075 10059 1. 06 O. OE+00 R17795. 1 EST_HUMAN yg09a02.r1 Soares infant brain 1NIB Homo sapiens cDNA clone IMAGE : 31652 5' Homo sapiens phosphoribosylglycinamide formyftransferase, phosphoribosytgfycinamide synthetase, 425 5463 10481 2. 62 O. OE+00 4503914 NT phosphoribosylaminoimidazole synthetase (GART) mRNA 426 5464 36. 55 O. OE+00 4506728 NT Homo sapiens ribosomal protein S5 (RPS5) mRNA 427 5465 10482 3. 49 0.0E+00 AB028942. 1 NT Homo sapiens mRNA for KIAA1019 protein, partial cds 428 5466 10483 6. 44 O. OE+00 4507152 NT Homo sapiens SON DNA binding protein (SON) mRNA 428 5466 10484 6. 44 O. OE+00 4507152 NT Homo sapiens SON DNA binding protein (SON) mRNA 429 5467 10485 3. 81 0.0E+00 AF193607. 1 NT Mus musculus truncated SON protein (Son) mRNA, complete cds 441 5478 0. 76 O. OE+00 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21 C001 443 5480 10498 3. 88 0.0E+00 4557879 NT Homo sapiens interferon gamma receptor 1 (IFNGR1) mRNA 448 5485 1. 31 O. 0E+00 AA324262. 1 EST HUMAN EST27054 Cerebellum II Homo sapiens cDNA 5'end 449 5486 2.44 0.0E+00 BE254447. 1 EST_HUMAN 601111520F1 NIH-MGC_16 Homo sapiens cDNA clone IMAGE : 3352348 5' 465 5502 10512 5. 86 O. OE+00 4504532 NT Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B) mRNA 465 5502 10513 5. 86 0.0E+00 4504532 NT Homo sapiens 5-hydroxytryptamine (serotonin) receptor 1B (HTR1B) mRNA 470 5506 10520 21. 4 O. OE+00 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA 470 5506 10521 21. 4 O. OE+00 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA 480 5517 10527 2. 88 0.0E+00 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 481 5518 10528 5. 35 0.0E+00 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 481 5518 10529 5. 35 0.0E+00 AL163246. 2 NT Homo sapiens chromosome 21 segment HS21 C046 490 5526 10534 3. 11 O. OE+00 AB033035. 1 NT Homo sapiens mRNA for KIAA1209 protein, partial cds 492 5528 10536 1.82 0.0E+00 AU132898. 1 EST HUMAN AU132898 NT2RP4 Homo sapiens cDNA clone NT2RP4000837 5' 500 5536 10542 4. 41 O. OE+00 BE385144. 1 EST_HUMAN 601274951F1 NIH_MGC_20 Homo sapiens cDNA clone IMAGE : 3615756 5' 501 7722 10543 4. 79 0.0E+00 AW938825. 1 ESTHUMAN PMO-DT0065-130400-002-c06 DT0065 Homo sapiens cDNA 504 5539 10545 1. 51 0.0E+00 AL117233. 1 NT Novel human gene mapping to chomosome 1 505 5540 10546 0. 81 O. OE+00 8923955 NT Homo sapiens PC326 protein (PC326), mRNA 508 5543 0. 68 O. OE+00 BF373403. 1 EST HUMAN IL2-FT0159-070800-120-F07 FT0159 Homo sapiens cDNA 515 5550 10555 4. 2 O. OE+00 AL163210. 2 NT Homo sapiens chromosome 21 segment HS21C010 522 7723 10559 1. 9 0.0E+00 BE081527. 1 EST HUMAN QV2-BT0635-160400-142-h05 BT0635 Homo sapiens cDNA 527 5562 10565 1.86 0.0E+00 BF028005.1 EST_HUMAN 601764858F1 NIH_MGC_53 Homo sapiens cDNA clone IMAGE : 3996998 5' 532 5567 10570 1. 25 0.0E+0 AB040909. 1 NT Homo sapiens mRNA for KIAA1476 protein, partial cds Page 177 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Databse Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value 535 5570 10573 17.56 0.0E+00 6006030 NT Homo sapiens transcription elongatin factor B (Sill), polypeptide 1-like (TCEB1L) mRNA 536 5571 10574 5. 39 O. OE+00 4504036 NT Homo sapiens guanine nucleotide binding protein (G protein), alpha 11 (Gq class) (GNA11) mRNA 536 5571 10575 5. 39 O. OE+00 4504036 NT Homo sapiens guanine nucleotide binding protein (G protein), alpha 11 (Gq class) (GNA11) mRNA 538 5573 10577 3. 44 O. OE+00 8923831 NT Homo sapiens anillin (LOC54443), mRNA 539 5574 10578 2. 66 O. OE+00 8923831 NT Homo sapiens anillin (LOC5443), mRNA 539 5574 10579 2. 66O. OE+00 8923831 NT Homo sapiens anillin (LOC54443), mRNA Homo sapiens X-linked anhidroitic ectodermal dysplasia protein gene (EDA), exon 2 and flanking repeat 544 5578 3. 9 O. OE+00 AF003528. 1 NT regions 552 5586 10588 1.35 0.0E+00 AW 135324. 1 EST_HUMAN UI-H-BI1-acb-h-04-0-Ul.s1 NCI_CGAP_Sub3 @ Homo sapiens cDNA clone IMAGE : 2713951 3' 561 5595 4. 5 O. OE+00 D10083. 1 NT Homo sapiens RGH1 gene, retrovirus-like element Homo sapiens ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 (UQCRFS1), nuclear gene 581 5613 10613 7. 89 O. OE+00 5174742 NT encoding mitochondrial protein, mRNA 594 5625 4. 24 O. OE+00 J04066. 1 NT Human apoplipoprotein A-i (ApoA-i) gene, exon 1 597 5628 10627 2. 06 O. OE+00 BF104898. 1 EST_HUMAN 601822627F1 NIH_MGC_75 Homo sapiens cDNA clone IMAGE:4045447 5' 599 5630 10629 1. 05 O. OE+00 8923631 NT Homo sapiens hypothetical protein FLJ20701 (FLJ20701), mRNA 599 5630 10630 1. 05 O. OE+00 8923631 NT Homo sapiens hypothetical protein FLJ20701 (FLJ20701), mRNA 600 5630 10629 0. 92 O. OE+00 8923631 NT Homo sapiens hypothetical protein FLJ20701 (FLJ20701), mRNA 600 5630 10630 0. 92 O. OE+00 8923631 NT Homo sapiens hypothetical protein FLJ20701 (FLJ207010, mRNA 601 5630 10629 0. 7 0.0E+00 8923631 NT Homo sapiens hypothetical protein FLJ20701 (FLJ20701), mRNA 601 5630 10630 0. 7 O. OE+00 8923631 NT Homo sapiens hypothetical protein FLJ20701 (FLJ20701), mRNA 610 5637 10638 0. 83 O. OE+00 AF221712. 1 NT Homo sapiens Smad-and Olf-interacting zinc finger protein mRNA, partial cds 610 5637 10639 0. 83 O. OE+00 AF221712. 1 NT Homo sapiens Smad-and Olf-interacting zinc finger protein mRNA, partial cds 617 5644 10646 2. 81 O. OE+00 AF149773. 1 NT Homo sapiens NOD1 protein (NOD1) gene, exons 1, 2, and 3 619 5646 10649 2. 39 O. OE+00 AB037807. 1 NT Homo sapiens mRNA for KIAA1386 protein, partial cds 621 5648 10650 0. 76 O. OE+00 6806918 NT Homo sapiens low density lipoprotein-related protein 2 (LRP2), mRNA 622 5649 10651 1. 52 0.0E+00 6806918 NT Homo sapiens low density lipoprotein-related protein 2 (LRP2), mRNA 622 5649 10652 1. 52 O. OE+00 6806918 NT Homo sapiens low density lipoprotein-related protein 2 (LRP2), mRNA 629 5657 10662 2. 63 O. OE+00 AA399486. 1 EST HUMAN zt60c07. r1 Soares_testis NHT Homo sapiens cDNA clone IMAGE : 726732 5' 633 5661 10666 6. 46 O. OE+00 D11078. 1 NT Homo sapiens RGH2 gene, retrovirus-like element 638 5666 3. 25 0.0E+00 4885526 NT Homo sapiens novel SH2-containing protein 3 (NSP3) mRNA 645 5673 10678 2. 37 0.0E+00 6006003 NT Homo sapiens glutamate receptor, ionotropic, N-methyl D-aspartate 2B (GRIN2B) mRNA 647 5675 10681 2. 08 O. OE+00 5031624 NT Homo sapiens CCAAT-box-binding transcription factor (CBF2) mRNA 650 5678 10685 3. 14 O. OE+00 U05235. 1 NT Human neutral amino acid transporter (ASCT1) gene, exon 8 654 5682 10688 1.1 0.0E+00 AF108389.1 NT Homo sapiens sodium/calcium exchanger isoform NaCa3 (NCX1) mRNA, complete cds Page 178 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession pabase Top Hit Descriptor 'DNO-. Signa) BTE No. Top H. t Descriptor NO : NO : NO : Signal BLAST E N Source. Value 654 5682 10689 1. 1 O. OE+00 AF108389. 1 NT Homo sapiens sodiumlcalcium exchanger isaform NaCa3 (NCX1) mRNA, complete cds 660 5687 10694 4. 21 O. OE+00 4826947 NT Homo sapiens protein kinase, X linked (PRKX) mRNA 660 5687 10695 4. 21 O. OE+00 4826947 NT Homo sapiens protein kinase, X-linked (PRKX) mRNA 666 7726 1. 79 O. OE+00 X57147. 1 NT Human endogenous retrovirus pHE. 1 (ERV9). 674 5699 10708 21. 88 O. OE+00 4504424 NT Homo sapiens high-mobility group (nonhistone chromosomal) protein 1 (HMG1) mRNA 678 5703 10711 15. 74 O. OE+00 AB029012. 1 NT Homo sapiens mRNA for KIAA1089 protein, partial cds 688 5712 10726 6. 92 O. OE+00 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM121 (POM121 L1), mRNA np49d01. s1 NCI CGAP Br1. 1 Homo sapiens cDNA clone IMAGE : 1129633 3'similar to gb : X57352 699 5723 10739 47. 96 O. OE+00 AA614537. 1 EST NUMAN INTERFERON-INDUCIBLE PROTEIN 1-8U (HUMAN) ; 703 5727 10743 4. 04 O. OE+00 M60675. 1 NT Human von W illebrand factor gene, exons 23 through 34 703 5727 10744 4. 04 O. OE+00 M60675. 1 NT Human von W illebrand factor gene, exons 23 through 34 713 5737 10753 1. 32 O. oE+00 5032192 NT Homo sapiens TNF receptor-associated factor 1 (TRAF1) mRNA 719 5742 10759 3. 44 O. OE+00 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds 719 5742 10760 3. 44 O. OE+00 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds 721 5744 10763 11. 53 O. OE+00 11545800 NT Homo sapiens hypothetical protein FLJ21634 (FLJ21634), mRNA TCAAP1D0779 Pediatric acute myelogenous leukemia cell (FAB M1) Baylor-HGSC project=TCAA Homo 727 5750 10771 2. 72 O. OE+00 BE241577. 1 EST HUMAN sapiens cDNA clone TCAAP0779 747 5769 10795 1. 78 O. OE+00 AF226990. 2 NT Homo sapiens MHC class I an6gen (HLA-G) mRNA, HLA-G1 allele, complete cds 747 5769 10796 1. 78 O. OE+00 AF226990. 2 NT Homo sapiens MHC class I antigen (HLA-G) mRNA, HLA-G1 allele, complete cds 750 5772 10799 3. 03 O. OE+00 J03764. 1 NT Human, plasminogen ac6vator inhibitor-1 gene, exons 2 to 9 750 5772 10800 3. 03 O. OE+00 J03764. 1 NT Human, plasminogen ac6vator inhibitx-1 gene, exons 2 to 9 752 5774 10801 1. 73 O. OE+00 AB037760. 1 NT Homo sapiens mRNA for KIAA1339 protein, partial cds 753 5775 10802 1. 35 O. OE+00 6912749 NT Homo sapiens zinc finger protein 212 (ZNF212), mRNA 755 7730 10804 4. 57 O. OE+00 D30612. 1 NT Homo sapiens mRNA for repressor protein, partial cds 756 5777 10805 5. 14 O. OE+00 BE869735. 1 EST_HUMAN 601445647F1 NIH_MGC 65 Homo sapiens cDNA clone IMAGE : 3849803 5' 760 5781 10809 3. 62 O. OE+00 R48915. 1 EST HUMAN yj69908. r1 Soares breast 2NbHBst Homo sapiens cDNA clone IMAGE : 154046 5' 761 5782 90810 5. 54 O. OE+00 5032086 NT Homo sapiens splicing factor 3a, subunit 1, 120kD (SF3A1), mRNA 770 5791 10819 1. 61 O. OE+00 AB011399. 1 NT Homo sapiens gene for AF-6, complete cds 773 5795 10823 3. 45 O. OE+00 7661965 NT Homo sapiens KIAA0170 gene product (KIAA0170), mRNA 784 5805 10835 1. 58 O. OE+00 D80006. 1 NT Human mRNA for KIAA0184 gene, partial cds 784 5805 10836 1. 58 O. OE+00 D80006. 1 NT Human mRNA for KIAA0184 gene, partial cds 789 5810 10840 2. 89 O. OE+00 X89772. 1 NT H. sapiens mRNA for interferon alphalbeta receptor (long form) 793 5814 10844 3. 13 O. OE+00 AB020717. 1 NT Homo sapiens mRNA for KIAA0910 protein, partia cds 793 5814 10845 3. 13 O. OE+00 AB020717. 1 NT Homo sapiens mRNA for KIAA0910 protein, part'al cds- . Page 179 of 209 Table 4 SinnlP Exon Prnhac Fxnracal in HRI 11111 f'. allc Mopst Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hilt SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: NO: Source Value 797 5818 10849 10.62 0.0E+00 5174478 NT Homo sapiens pericentrin (PCNT) mRNA 798 5819 1 87.45 0.0E+00 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA 814 5835 10870 1. 57 O. OE+00 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 815 5836 10871 2. 15 O. OE+00 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 817 5838 10873 1.84 0.0E+00 4557686 NT Homo Sapiens potassium vollage-gated channel, Isk-related family, member 1/(KCNE1) mRNA 823 5843 10879 1.98 0.0E+00 AF1008830.1 NT Homo sapiens serine-threonine protein kinase (MNBH) mRNA. complete cds 8231 58431 10880 1. 98 O. OE+00 AF108830. 1 NT Homo sapiens serine-threonine protein kinase (MNBH) mRNA, complete cds 824 5844 10881 1. 05 O. OE+00 AF108830. 1 NT Homo sapiens serine-threonine protein kinase (MNBH) mRNA, complete cds 829 5849 10886 1. 72 O. OE+00 4503854 NT Homo sapiens GA-binding protein transcription factor, alpha subunit (60kD) (GABPA), mRNA 833 5852 10891 2. 11 O. OE+00 4507500 NT Homo sapiens T-cell lymphoma invasion and metastasis 1 (T1AM1) mRNA 833 5852 10892 2. 11 O. OE+00 4507500 NT Homo sapiens T-cell lymphom invasion and metastasis 1 (TIAM1) mRNA 840 5859 1. 37 O. OE+00 AF027153. 1 NT Homo sapiens sodium/myo-inositol cotransporter (SLC5A3) gene, complete cds 844 5863 10903 4. 35 O. OE+00 AB028942. 1 NT Homo saipens mRNA for KIAA1019 protein, partial cds 844 5863 10904 4. 35 O. OE+00 AB028942. 1 NT Homo sapiens mRNA for KIAA1019 protein, partial cds 845 5864 10905 11. 54 O. OE+00 4507152 NT Homo sapiens SON DNA binding protein (SON) mRNA 846 5865 10906 4. 49 O. OE+00 AB028942. 1 NT Homo sapiens mRNA for KIAA1019 protein, partial cds 847 5866 10907 28. 07 O. OE+00 4506728 NT Homo sapiens ribosomal protein S5 (RPS5) mRNA 850 5869 10910 1.1 0.0E+00 AB020717. 1 NT Homo sapiens mRNA for KIAA0910 protein, partial cds 850 5869 10911 1. 1 O. OE+00 AB020717. 1 NT Homo sapiens mRNA for Kit0910 protein, partial cds 851 5870 10912 1. 78 O. OE+00 AA533272. 1 EST HUMAN nj66d07. s1 NCI CGAP Pr10 Homo sapiens cDNA clone IMAGE : 997453 851 5870 10913 1.78 0.0E+00 AA533272. 1 EST_HUMAN nj66d07.s1 NCl_CGAP_Pr10 Homo sapiens cDNA clone IMAGE : 997453 852 5871 6.52 0.0E+00 BF677694. 1 EST HUMAN 602085579F1 NIH MGC 83 Homo sapiens cDNA clone IMAGE : 4249915 5' 856 5875 10914 1. 36 0.0E+11 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 856 5875 10915 1. 36 0.0E+00 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 857 5876 10916 2. 29 0.0E+00 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 857 5876 10917 2. 29 00E+00 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 880 5898 10940 1. 27 00E+00 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 887 5905 10945 1.4 00E+00 BE089592. 1 EST_HUMAN QVO-BT0703-280400-211-g11 BT0703 Homo sapiens cDNA 887 5905 10946 1. 4 00E+00 BE089592. 1 EST HUMAN QVO-BT0703-280400-211-g11 BT0703 Homo sapiens cDNA 897 5915 10955 2. 23 00E+00 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 907 5024 9.258 00E+00 4504958 NT Homo sapiens laminin recetpro 1 (67kD, ribosomal prtein SA) (LAMR1), mRNA 910 5924 105. 51 O. OE+00 4504958 NT Homo sapiens laminin receptor 1 (67kD, ribosomal protein SA) (LAMR1), mRNA 911 5927 10962 1. 55 O. OE+00 AF089747. 1 NT Homo sapiens alpha-1-antichymotrypsin precursor, mRNA, partial cds 912 5928 10963 1. 39 O. OE+00 L28101. 1 NT Homo sapiens kallistatin (Pl4) gene, exons 1-4, complete cds Page 180 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Top Hit Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit Descriptor SEQ ID SEQ ID Database ID NO: Signal BLAST E No. NO: NO: Source Value 936 5954 10985 1. 21 O. OE+00 M37190. 1 NT Human ras inhibitor mRNA, 3'end 937 5954 10986 6. 98 O. OE+00 M37190. 1 NT Human ras inhibitor mRNA, 3'end 938 5955 10987 0. 78 O. OE+00 M37190. 1 NT Human ras inhibitor mRNA, 3'end 939 5956 10988 1. 55 O. OE+00 4507430 NT Homo sapiens thyrotrophic embryonic factor (TEF), mRNA 939 5956 10989 1. 55 O. OE+00 4507430 NT Homo sapiens thyrotrophic embryonic factor (TEF), mRNA 947 7735 10996 1.94 00E+00 A1001948.1 EST_HUMAN os98e03.s1 NCl_CGAP_GC3 Homo sapiens cDNA clone IMAGE : 1613404 3' 947 7735 10997 1. 94 O. OE+00 A1001948. 1 EST HUMAN os98e03. s1 NCI CGAP GC3 Homo sapiens cDNA clone IMAGE : 1613404 3' 949 5965 10999 7. 69 O. OE+00 7657266 NT Homo sapiens KIAA0929 protein Msx2 interacting nuclear target (MINT) homolog (KIAA0929), mRNA 960 5975 11009 1.89 00E+00 AB030566. 1 NT Homo sapiens mRNA for PSP24, complete cds 968 5983 11015 1. 14 O. OE+00 BF366974. 1 EST_HUMAN PM2-GN0014-050900-001-f02 GN0014 Homo sapiens cDNA 968 5983 11016 1. 14 O. OE+00 BF366974. 1 EST_HUMAN PM2-GN0014-050900-001-f02 GN0014 Homo sapiens cDNA 968 5983 11017 1. 14 O. OE+00 BF366974. 1 EST_HUMAN PM2-GN0014-050900-001-f02 GN0014 Homo sapiens cDNA 969 5984 11018 1. 55 O. OE+00 X52207. 1 NT Homo sapiens partial c-fgr gene, exons 2 and 3 969 5984 11019 1. 55 O. OE+00 X52207. 1 NT Homo sapiens partial c-fgr gene, exons 2 and 3 978 5993 11026 2. 93 O. OE+00 4757969 NT Homo sapiens chromodomain protein, Y chromosome-like (CDYL) mRNA 989 6003 11034 3.09 00E+00 U83668. 1 NT Human beta-tubulin (TUB4q) gene, complete cds 990 6004 11035 58. 93 O. OE+00 U83668. 1 NT Human beta-tubulin (TUB4q) gene, complete cds 991 6004 11035 27.22 00E+00 u83668. 1 NT Human beta-tubulin (TUB4q) gene, complete cds 994 6007 5. 92 O. OE+00 AF198490. 1 NT Homo sapiens 8q22. 1 region and MTG8 (CBFA2T1) gene, partial cds 995 6007 11.99 00E+00 AF198490. 1 NT Homo sapiens 8q22. 1 region and MTG8 (CBFA2T1) gene, partial cds 998 6010 11040 1. 12 00E+00 AF111170. 3 NT Homo sapiens 14q32 Jagged2 gene, complete cds ; and unknown gene 999 6010 11040 1. 89 O. OE+00 AF111170. 3 NT Homo sapiens 14q32 Jagged2 gene, complete cds ; and unknown gene 1000 6010 11040 1. 74 00E+00 AF111170. 3 NT Homo sapiens 14q32 Jagged2 gene, complete cds ; and unknown gene 1001 6011 11041 6. 04 00E+00 AF111170. 3 NT Homo sapiens 14q32 Jagged2 gene, complete cds ; and unknown gene 1004 6014 11044 2. 62 O. OE+00 7661685 NT Homo sapiens DKFZP586M0122 protein (DKFZP586M0122), mRNA 1008 6018 11048 4. 81 O. OE+00 5803114 NT Homo sapiens inner membrane protein, mitochonidrial (mitofilin) (IMMT), mRNA aa36g07 .s1 Stratagene fetal retina 937202 Homo sapiens cDNA clone IMAGE : 838236 3'similar to 1009 6019 4. 43 O. OE+00 AA458680. 1 EST HUMAN SW : PRS8 HUMAN P47210 26S PROTEASE REGULATORYSUBUNIT 8 ; EST51i24 WATM1 Homo sapiens cDNA clone 51i24 similarto DNA-DIRECTED RNA POLYMERASE II 1012 6022 11052 0. 8 O. OE+00 N43182. 1 EST_HUMAN (alignemt Ser and Pro with BLASTx or p) EST51i24 WATM1 Homo sapiens cDNA clone 51i24 similar to DNA-DIRECTED RNA POLYMERASE II 1012 6022 11053 0.8 00E+00 N43182. 1 EST HUMAN (alignment Ser and Pro with BLASTx or p) 1013 6023 11054 1. 08 00E+00 4759249 NT Homo sapiens TRAF family member-associated NFKB activator (TANK) mRNA Page 181 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source NO: NO: Source Value 1013 6023 11055 1.08 00E+00 4759249 NT Homo Sapiene TRAF femily member-associated NFKB aclivator (TANK) mRNA 1016 6026 5. 63 O. OE+00 8922933 NT Homo sapiens hypothetical protein FLJ11196 (FLJ11196), mRNA 1030 6040 11070 10 O. OE+00 4758569 NT Homo sapiens heat shock 70kD protein 9B (mortalin-2) (HSPA9B) mRNA 1048 6057 11085 3. 04 O. OE+00 4826672 NT Homo sapiens cadherin 6, K-cadherin (fetal kidney) (CDH6) mRNA 1048 6057 11086 3. 04 O. OE+00 4826672 NT Homo sapiens cadherin 6, K-cadherin (fetal kidney) (CDH6) mRNA 1052 6061 11090 3. 86 O. OE+00 8923624 NT Homo sapiens hypothetical protein FLJ20695 (FLJ20695), mRNA 1052 6061 11091 3. 86 O. OE+00 8923624 NT Homo sapiens hypothetical protein FLJ20695 (FLJ20695), mRNA 1053 6062 11092 127. 96 00E+00 AJ245922. 1 NT Homo sapiens mRNA foralpha-tubulin 8 (TUBAS gene) 1055 6064 1. 04 O. OE+00 8923087 NT Homo sapiens hypothecal protein FLJ20080 (FLJ20080), mRNA 1057 6066 11096 4. 28 O. OE+00 5174384 NT Homo sapiens alkylation repair ; alkB homolog (ABH), mRNA 1066607411107 3. 16 O. OE+00 4758117 NT Homo sapiens Death associated protein 3 (DAP3) mRNA 1079 6086 11115 5. 76 00E+00 BE005208:1 EST_HUMAN MRO-BN0115-200300-003-h09 BN0115 Homo sapiens cDNA 1102 6109 11139 4. 27 O. OE+00 7706134 NT Homo sapiens potassium channel, subfamily K, member 9 (KCNK9), mRNA 1102 6109 11140 4. 27 0. OE+00 7706134 NT Homo sapiens potassium channel, subfamily K, member 9 (KCNK9), mRNA 1115 6121 11150 1. 67 O. OE+00 4826947 NT Homo sapiens protein kinase, X-linked (PRKX) mRNA 1115 6121 11151 1. 67 O. OE+00 4826947 NT Homo sapiens protein kinase, X-linked (PRKX) mRNA 1116 6122 11152 46. 69 00E+00 4506712 NT Homo sapiens ribosomal protein S27a (RPS27A) mRNA 1118 6124 11154 2. 22 O. OE+00 8923290 NT Homo sapiens hypothetical protein FLJ20309 (FLJ20309), mRNA 1121 6127 11157 37. 23 00E+00AB002059. 1 NT Homo sapiens DNA for Human P2XM, complete cds 1123 6129 11158 82. 18 00E+00 AB002059.1 NT Homo sapiens DNA for Human P2XM, complete cds 1124 6130 11159 4.76 00E+00 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM121 (POM121 L1), mRNA 1124 6130 11160 4. 76 O. OE+00 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM121 (POM121L1),mRNA 1127 6132 11162 4. 36 O. OE+00 7706500 NT Homo sapiens Npw38-binding protein NpwBP (LOC51729), mRNA 1128 6133 11163 0. 66 00E+00 X95826. 1 NT H. sapiens ART4 gene 1128 6133 11164 0. 66 O. OE+00 X95826. 1 NT H. sapiens ART4 gene 1129 6134 11165 1.23 00E+00 Al147650. 1 ESTHUMAN qb22d10. x1 SoarespregnantuterusNbHPU Homo sapiens cDNA clone IMAGE:1697011 3' 1131 6136 11167 3.06 00E+00 AB020710. 1 NT Homo sapiens mRNA for KIAA0903. protein, partial cds 1138 6143 11173 0. 86 O. OE+00 4758081 NT Homo sapiens chondroitin sulfate proteoglycan 2 (versican) (CSPG2) mRNA 1138 6143 11174 0. 86 O. OE+00 4758081 NT Homo sapiens chondroitin sulfate proteoglycan 2 (versican) (CSPG2) mRNA 1139 6144 11175 1. 05 O. OE+00 9966844 NT Homo sapiens chromosome 12 open reading frame 3 (C120RF3), mRNA 1150 6154 11186 5. 48 00E+00 7305076 NT Homo sapiens glutamate decarboxylase 1 (brain, 67kD) (GAD1), transcript variant GAD25, mRNA 1150 6154 11187 5. 48 O. OE+00 7305076 NT Homo sapiens glutamate decarboxylase 1 (brain, 67kD) (GAD1), transcript variant GAD25, mRNA 1152 6156 11189 0. 98 O. OE+00 AB037835. 1 NT Homo sapiens mRNA for KIAA1414 protein, partial cds 1159 6163 11198 14. 52 00E+00 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA Page 182 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exom Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit NO : Signa ! BLAST E No. Database Top Hit Descriptor Value f 6175 11209 1.01 00E+00 AF073299. 1 NT Homo sapiens Na+/H+ exchanger isoform 2 (NHE2) mRNA, complete cds 1190 6191 1. 32 O. OE+00 7657336 NT Homo sapiens mutL (E. cdi) homolog 3 (MLH3), mRNA 1203 6204 11241 0. 69 O. OE+00 8922593 NT Homo sapiens hypothetical protein FLJ10697 (FLJ10697), mRNA 1206 6207 11244 0. 89 00E+00 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds 1206 6207 11245 0. 89 00E+00 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds 1207 6208 11246 1. 55 00E+00 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds 1208 7741 11247 1. 09 O. OE+00 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds 1226 6226 11272 4. 89 00E+00 AF109718. 1 NT Homo sapiens chromosome 3 subtelomeric region 1228 6227 11273 2. 52 00E+00 4503098 NT Homo sapiens chondroitin sulfate proteoglycan 4 (melanoma-associated) (CSPG4), mRNA 1238 6236 11279 4. 31 O. OE+00 4505740 NT Homo sapiens prefoldin 4 (PFDN4) mRNA 1247 6245 2. 72 O. OE+00 Y18000. 1 NT Homo sapiens NF2 gene 1255 6253 11294 283. 18 00E+00 4506718 NT Homo sapiens ribosomal protein S2 (RPS2) mRNA 1262 6260 11303 5. 64 00E+00 AF084479. 1 NT Homo sapiens Williams-Beuren syndrome deletion transcript 9 (WBSCR9) mRNA, complete cds 1268 6266 11307 1. 42 00E+00 AB040940. 1 NT Homo sapiens mRNA for KIAA1507 protein, partial cds 1268 6266 11308 1.42 00E+00 AB040940. 1 NT Homo sapiens mRNA for KIAA1507 protein, partial cds 1281 6280 11321 2. 45 00E+00 5174748 NT Homo sapiens Wolfram syndrome (WFS) mRNA 1281 6280 11322 2. 45 O. OE+00 5174748 NT Homo sapiens Wolfram syndrome (WFS) mRNA 1281 6280 11323 2. 45 00E+00 5174748 NT Homo sapiens Wolfram syndrome (W FS) mRNA 1282 6281 2.35 00E+00 AF096156. 1 NT Homo sapiens protein phosphatase 2A BR gamma subunit gene, exon 5 1292 7743 11335 1. 16 O. OE+00 7657529 NT Homo sapiens rhabdoid tumor deletion region protein 1 (RTDR1), mRNA 1292 7743 11336 1. 16 00E+00 7657529 NT Homo sapiens rhabdoid tumor deletion region rptoein 1(RTDR1), mRNA 1296 6294 11341 1. 37 00E+00 5803146 NT Homo sapiens ring finger protein 9 (RNF9), mRNA 1297 6295 11342 2. 26 00E+00 4508004 NT Homo saopiens zinc finger protein 173 (ZNF173) mRNA 1299 6297 11343 0. 67 O. OE+00 5803146 NT Homo sapiens ring finger protein 9 (RNF9), mRNA 1300 6298 11344 2. 07 00E+00 4508004 NT Homo sapiens zinc finger protien 173 (ZNF173) mRNA 1302 6300 11346 4. 17 00E+00 AB011149. 1 NT Homo sapiens mRNA for KIAA0577 protein, complete cds 1303 6301 11347 9. 28 O. OE+00 7661965 NT Homo sapiens KIAA0170 gene product (KIAA0170), mRNA 1304 6302 11348 10. 18 00E+00 761965 NT Homo sapiens KIAA0170 gene product (KIAA0170), mRNA 1305 6303 11349 3. 67 00E+00 8567387 NT Homo sapiens period (Drosophila) homolog 3 (PER3), mRNA 1305 6303 11350 3. 67 O. OE+00 8567387 NT homo sapiens period (Dorsophila) homolog 3(PER3), mRNA 1317 6314 1.22 00E+00 N14123. 1 NT Human endogenous retrovirus HERV-K10 1375 6372 11421 1.64 00E+00 BE257955. 1 EST_HUMAN 6011097292F1 NIH_MGC_16 Homo sapiens cDNA cione IMAGE : 3350471 5' 1375 6372 11422 1.64 0. OE+00 BE257955. 1 EST HUMAN 601109792F1 NIH MGC 16 Homo sapiens cDNA clone IMAGE : 3350471 5' 1384 6381 11431 1.51 00E+00 AJ250014. 1 NT Homo sapiens mRNA for Familial Cylindromatosis cyld gene Page 183 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession ID NO : Signal BLAST E No, NO : NO : Value Source. qg38b06. x1 Soares tes6s NHT Homo sapiens cDNA clone IMAGE : 1837427 3'similar to W P : T27A1. 5 1392 6389 11442 1. 16 O. OE+00 AI208756. 1 EST HUMAN CE14213 ; 1393 6390 11443 28. 34 O. OE+00 6042206 NT RAN, member RAS oncogene familyHomo sapiens RAN, member RAS oncogene family (RAN), mRNA 1401 6398 11453 1. 59 O. OE+00 4505646 NT Homo sapiens proprotein convertase subtilisinlkepn typa 2 (PCSK2) mRNA 1401 6398 11454 1. 59 O. OE+00 4505646 NT Homo sapiens proprotein convertase subtilisinlkebn type 2 (PCSK2) mRNA 1403 6400 11457 3. 9 O. OE+00 7705565 NT Homo sapiens KIAA1114 protein (KIAA1114), mRNA 1403 6400 11458 3. 9 O. OE+00 7705565 NT Homo sapiens KAA1114 protein (KIAA1114), mRNA 1405 6402 11459 4. 32 O. OE+00 AJ238093. 1 NT Homo sapiens partial AF-4 gene, exons 2 to 7 and Alu repeat elements 1413 6411 11470 3. 56 O. OE+00 AF038280. 1 NT Homo sapiens alpha1-6fucosyltransferase (alpha1-6FucT) gene, exon 7 1434 6431 11487 2. 12 O. OE+00 AL132999. 1 NT Novel human gene on chromosome 20 1435 6432 11488 1. 3 O. OE+00 AL137764. 1 NT Novel human gene mapping to chomosome 1 1439 6436 11493 1. 69 O. OE+00 D87077. 1 NT Human mRNA for KIAA0240 gene, partial cds 1442 6439 11496 6. 31 O. OE+00 6912457 NT Homo sapiens calcineurin binding protein 1 (KIAA0330), mRNA 1444 6441 11498 2. 08 O. OE+00 7661965 NT Homo sapiens KIAA0170 gene product (KIAA0170), mRNA 1444 6441 11499 2. 08 O. OE+00 7661965 NT Homo sapiens KIAA0170 gene product (KIAA0170), mRNA 1480 6477 11533 3. 1 O. OE+00 7706434 NT Homo sapiens hHDC for homolog of Drosophila headcase (LOC51696), mRNA 1493 6491 11546 0. 99 O. OE+00 AW959687. 1 EST_HUMAN EST371757 MAGE resequences, MAGF Homo sapiens cDNA 1494 6492 11547 2. 99 O. OE+00 AA481172. 1 EST HUMAN aa34a03. r1 NCI_CGAP GCB1 Homo sapiens cDNA clone IMAGE : 815116 5' 1500 6498 11551 42. 31 O. OE+00 AF023860. 1 NT Cercopithecus aethiops cyclophilin A mRNA, complete cds 1500 6498 11552 42. 31 O. OE+00 AF023860. 1 NT Cercopithecus aethiops cyclophilin A mRNA, complete cds 1502 6500 11555 1. 1 O. OE+00 AW976097. 1 EST HUMAN EST388206 MAGE resequences, MAGN Homo sapiens cDNA 1502 6500 11556 1. 1 O. OE+00 AW 976097. 1 EST HUMAN EST388206 MAGE resequences, MAGN Homo sapiens cDNA- 1503 6501 11557 1. 11 O. OE+00 D10884. 1 NT Bovine mRNA for neurocalcin = Homo sapiens Bruton's tyrosine kinase (BTK), alpha-D-galactosidasa A (GLA), L44-like ribosomal protein : ; 1505 6503 1. 87 O. OE+00 U78027. 1 NT (L44L) and FTP3 (FTP3) genes, complete cds 9506 6504 11560 2. 02 O. OE+00 4505404 NT Homo sapiens transmembrane glycoprotein (GPNMB) mRNA 1506 6504 11561 2. 02 O. OE+o0 4505404 NT Homo sapiens transmembrane glycoprotein (GPNMB) mRNA 1507 6505 11562 2. 55 O. OE+00 7662405 NT Homo sapiens KIAA0957 protein (KIAA0957), mRNA 1508 6506 7. 44 O. OE+00 7656972 NT Homo sapiens TNF-inducible protein CG12-1 (CG12-1), mRNA 1513 6511 11568 3 O. OE+00 M98478. 1 NT Human transglutaminase mRNA, complete cds 1516 6514 11570 1. 08 O. OE+00 4507720 NT Homo sapiens titin (TTN) mRNA 1516 6514 11571 1. 08 O. OE+00 4507720 NT Homo sapiens titin (TTN) mRNA 1517 7749 39. 26 O. OE+00 4506654 NT Homo sapiens ribosomal protein L5 (RPL5) mRNA Page 184 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit NO: NO: ID NO: Singal BLAST E No. Source Value 1518 6515 11572 61. 77 O. OE+00 M14199. 1 NT Human laminin receptor (2H5 epitope) mRNA. 5'end 1530 6528 11587 8. 55 O. OE+00 4503098 NT Homo sapiens chondroitin suffate proteoglycan 4 (melanoma-associated) (CSPG4), mRNA 1537 6535 1. 85 O. OE+00 D00333. 1 NT human c-yes-2 gene 1545 6543 11601 26. 28 O. OE+00 Z83738. 1 NT H. sapiens hH2B/e gene 1546 6544 11602 2. 84 O. OE+00 5921460 NT Homo sapiens butyrophilin, subfamily 2, member A1 (BTN2A1), mRNA 15346 6544 11603 2.84 0. OE+00 5921460 NT Homo sapiens butyrophilin, subfamily2, memberAl (BTN2A1), mRNA 1547 6545 11604 6. 07 O. OE+00 AV690831. 1 EST HUMAN AV690831 GKC Homo sapiens cDNA clone GKCBOF02 5' 1547 6545 6.07 0. OE+00 AV690831. 1 EST HUMAN AV690831 GKC Homo sapiens cDNA clone GKCBOF02 5' 1549 7750 11608 1. 72 00E+00 AB040905. 1 NT Homo sapiens mRNA for KIAA1472 protein, partial cds 1553 6550 11609 2. 77 O. OE+00 AF157476. 1 NT Homo sapiens DNA polymerase zeta catalytic subunit (REV3) mRNA, complete cds 1555 6552 11612 6.44 00E+00 7662183 NT Homo sapiens KIAA0569 gene product (KIAA0569), mRNA 1555 6552 11613 6. 44 O. OE+00 7662183 NT Homo sapiens KIAA0569 gene product (KIAA0569), mRNA 1557 6554 11614 84. 9 O. OE+00 5729876 NT Homo sapiens heat shock 70kD protein 10 (HSC71) (HSPA10), mRNA 1557 6554 11615 84.9 00E+00 5729876 NT Homo sapiens heat shock 70kD protein 10 (HSC71) (HSPA10), mRNA 1559 6556 11617 2. 3 O. OE+00 M91803. 1 NT Human sodium channel mRNA 1572 6569 11631 9. 86 O. OE+00 H26973. 1 EST_HUMAN yo76c05.s1 Soares aduit brain N2b4HB55Y Homo sapiens cDNA clone IMAGE : 183848 3' 1579 6576 11639 1. 95 O. OE+00 AB046829. 1 NT Homo sapiens mRNA for KIAA1609 protein, partial cds 1579 6576 11640 1. 95 O. OE+00 AB046829. 1 NT Homo sapiens mRNA for KIAA1609 protein, partial cds 1598 6594 11655 4. 22 O. OE+00 AW444637. 1 EST HUMAN Ui-H-B13-ajw-c-04-0-Ul. s1 NCI_CGAP Sub5 Homo sapiens cDNA clone IMAGE : 2733294 3' 1625 6622 11690 8. 38 O. OE+00 BE144364. 1 ESTHUMAN MRO-HT0166-191199-004-b11 HT0166 Homo sapiens cDNA 1625 6622 11691 8.38 00E+00 BE144364. 1 EST_HUMAN MRO-HT0166-191199-004-b11 HT0166 Homo sapiens cDNA wg81b07. x1 SoaresNSFF89WOTPAPS1 Homo sapiens cDNA clone IMAGE : 2371477 3'similar to 1629 6626 11695 3.34 00E+00 A1768104.1 EST_HUMAN TR:Q62788 CYS2/HIS2 ZINC FINGER PROTEIN.; 1630 6627 11698 1.18 00E+00 4758513 Homo sapiens hematopoietic-derviced zinc finger protein (HD-ZNF1) mRNA 1631 6628 11697 2. 39 00E+00 AF057177. 1 NT Homo sapiens T-cell receptor gamma V1 gene region 1634 6631 11700 2. 07 00E+00 M29580. 1 NT Human zinc-finger protein 7 (ZFP7) mRNA, complete cds 1634 6631 11701 2.07 0. OE+00 M29580. 1 NT Human zinc-finger protein 7 (ZFP7) mRNA, complete cds 1636 6633 11703 26. 94 O. OE+00 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA 1637 6634 11704 1. 45 0. OE+00 7657065 NT Homo sapiens v-ets avian erythroblastosis virus E26 oncogene related (ERG), mRNA hu1do5.x1 NCl_CGAP_Lu24 Homo sapiens cDNA clone IMAGE : 3166281 3'similar to TR : 095147 095147 1641 6638 11707 2. 18 00E+00 BE222374. 1 EST HUMAN MKP-1 LIKE PROTEIN TYROSINE PHOSPHATASE ; hul 1d05.x1 NCl CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3166281 3'similar to TR : 095147 095147 1641 6638 11708 2. 18 O. OE+00 BE222374. 1 EST HUMAN MKP-1 LIKE PROTEIN TYROSINE PHOSPHATASE ; 1643 6639 11710 1. 29O. OE+00 4557610 NTHomo sapiens gamma-aminobutyric acid (GABA) A receptor, gamma 2 (GABRG2) mRNA Page 185 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Most SImilar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit Descriptor SEQ ID SEQ ID Database NO: NO: ID NO: Signal BALSTE No. gerce Value yo59e08.r1 Soares breast 3NbHBst Homo sapiens cDNA clone IMAGE: 182246 5' similer to gb: M64099 1646 6642 11713 8. 67 O. OE+00 H30132. 1 EST HUMAN GAMMA-GLUTAMYLTRANSPEPTIDASE 5 PRECURSOR (HUMAN) ; yo59e08. r1 Scares breast 3NbHBst Homo sapiens cDNA clone IMAGE : 182246 5'similar to gb : M64099 1646 6642 11714 8. 67 O. OE+00 H30132. 1 EST HUMAN GAMMA-GLUTAMYLTRANSPEPTIDASE 5 PRECURSOR (HUMAN) ; 1648 6644 11716 19. 26 O. OE+00 Z80780. 1 NT H. sapiens H2B/h gene 1648 6644 11717 19. 26 O. OE+00 Z80780. 1 NT H. sapiens H2B/h gene 1651 6647 64. 75 O. OE+00 5031748 NT Homo sapiens high-mobility group (nonhistone chromosomal) protein 17 (HMG17), mRNA 1660 6656 11730 5. 23 00E+00 8923841 NT Homo sapiens FOXJ2 forkhead factor (LOC55810), mRNA 1663 6659 11733 1. 85 O. OE+00 5453855 NT Homo sapiens pericentriolar material 1 (PCM1) mRNA 1669 6665 11741 1. 1 O. OE+00 4826973 NT Homo sapiens RNA binding motif protein, Y choromosome, family 1, member A1 (RBMY1A1) mRNA 1675 6671 11748 7. 62 O. OE+00 AB026542. 1 NT Homo sapiens WAVE2 mRNA for WASP-family protein, complete cds 1677 6673 1. 82 O. OE+00 S94400. 1 NT TCR zeta [human Genomic/mRNA, 365 nt, segment 1 of 8] 1686 6682 11757 1. 82 O. OE+00 4557538 NT Homo sapiens solute carrier family 26 (sulfate transporter), member 2 (SLC26A2) mRNA 1693 7753 11764 1. 05 O. OE+00 11545911 NT Homo sapiens NOD2 protein (NOD2), mRNA 1706 6701 11778 2. 59 O. OE+00 AF273841. 1 NT Homo sapiens SMCY (SMCY) gene, complete cds 17477754150. 64O. OE+00 4506718 NT Homo sapiens ribosomai protein S2 (RPS2) mRNA 1752 6746 11826 3. 32 O. OE+00 4557556 NT Homo sapiens E1A binding protein p300 (EP300) mRNA 1752 6746 11827 3. 32 O. OE+00 4557556 NT Homo sapiens E1A binding protein p300 (EP300) mRNA 1755 6748 11831 2. 75 O. OE+00 U63963. 1 NT Human CSF-1 receptor (FMS) gene, complete cds, and (SMF) gene, partial cds 1759 7755 11836 8. 02 00E+00 4505332 NT Homo sapiens nuclear autoantigenic sperm protein (histone-binding) (NASP) mRNA 1771 6763 11851 22. 06 O. OE+00 U14967. 1 NT Human ribosomal protein L21 mRNA, complete cds 1773 6765 11854 12.66 00E+00 $AB002331.1 NT Human mRNA for KIAA033 gene, aprtial cds 1774 6766 11855 13. 86 00E+00 4502264 NT Homo sapiens activating transcription factor 4 (tax-responsive enhancer element B67) (ATF4) mRNA 1774 6766 11856 13. 86 00E+00 4502264 NT Homo sapiens activating transcription factor 4 (tax-responsive enhancer element B67) (ATF4) mRNA 1774 6766 11857 13.86 00E+00 4502264 NT Homo sapiens activating transacription factor 4 (tax-responsive enhancer element (B67) (ATF4) mRNA 1788 6780 11872 1.29 00E+00 4504625 NT Homo sapiens immunoglobin superfamily, member 3 (IGSF3) mRNA, and transiated products 1788 6780 91873 1. 29 O. OE+00 4504626 NT Homo saplens immunoglobin sperfamily, member 3 (IGSF3) mRNA, and translated products 1798 6789 11878 5. 58 O. OE+00 6005855 NT Homo sapiens Retina-derived POU-domain factor-1 (RPF-1), mRNA 1798 6789 11879 5. 58 00E+00 6005855 NT Homo sapiens Retina-derived POU-domain factor-1 (RPF-1), mRNA 1807 6798 11888 3. 27 O. OE+00 AB032978. 1 NT Homo sapiens mRNA for KIAA1152 protein, partial cds 1807 6798 11889 3. 27 00E+00 AB032978.1 NT Homo sapiens mRNA for KIAA1152 protein, partial cds Page 186 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID @@@ Database ID NO: I Signal BLAST E No. Source Value 1811 6801 11891 3. 31 O. OE+00 4826783 NT Homo sapiens potassium voltage-gated channel, Shab-related subfamily, member 1 (KCNB1) mRNA 1811 6801 11892 3. 31 O. OE+00 4826783 NT Homo sapiens potassium voltage-gated channel, Shab-related subfamily, member 1 (KCNB1) mRNA 1812 6802 11893 8. 06 O. OE+00 U07147. 1 NT Human retinal degeneration slow (RDS) gene, exon 1 1812 6802 11894 8. 06 O. OE+00 U07147. 1 NT Human retinal degeneration slow (RDS) gene, exon 1 1815 6805 11897 1. 53 O. OE+00 AW207280. 1 EST_HUMAN UI-H=BI1-afn-f-07-0-UI.s1 NCl_CGAP_Sub3 Homo sapiens cDNA clone IMAGE : 2722333 3' 1815 6805 11898 1.53 00E+00 AW207280. 1 EST_HUMAN UI-H-Bl1-afn-f-07-0-UI.s1. NCl_CGAP_Sub3 Homo sapiens cDNA clone IMAGE : 2722333 3' 1837 6827 11915 2. 87 O. OE+00 BE277465. 1 EST HUMAN 601179164F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3547239 5' 1837 6827 11916 2. 87 O. OE+00 BE277465. 1 EST_HUMAn 601179164F1 NIH_MCG_20 Homo sapiens cDNA clone IMAGE : 3547239 5' 1878 6867 11955 1. 79 O. OE+00 7657390 NT Homo sapiens nuclear protein (NP220), mRNA 1878 6867 11956 1.79 00E+00 7657390 NT Homo sapiens nuclear protein (NP220), mRNA 1881 6870 11958 2.53 00E+00 4506384 NT Homo sapiens RAD1 (S. pombe) homolog (RAD1) mRNA, and translated products 1881 6870 11959 2.53 00E+00 4506284 NT Homo sapiens RAD1 (S. pombe) homolog (RAD1) and translated products 1888 6877 11967 1. 87 O. OE+00 AB037788. 1 NT Homo sapiens mRNA for KIAA1367 protein, partial cds 1891 6880 1. 55 O. OE+00 AF157476. 1 NT Homo sapiens DNA polymerase zeta catalytic subunit (REV3) mRNA, complete cds 1892 7758 11970 1. 49 O. OE+00 M98478. 1 NT Human transglutaimase mRNA, complete cds 1892 7758 11971 1.49 00E+00 M98478. 1 NT Human transgultaimase mRNA, complete cds 1897 6885 11978 1.31 00E+00 4507464 NT Homo sapiens transforming growth factor, beta 3 (TGFB3), mRNA 1897 6885 11979 1. 31 O. OE+00 4507464 NT Homo sapiens transforming growth factor, beta 3 (TGFB3), mRNA 1900 6887 11981 1. 17 O. OE+00 7657038 NT Homo sapiens death receptor 6 (DR6), mRNA Homo sapiens glutathione S-transferase theta 2 (GSTT2) and glutathione S-transferase theta 1 (GSTT1) 1902 6889 6. 49 O. OE+00 AF240786. 1 NT genes, complete cds 1907 6894 3. 82 O. OE+00 M55632. 1 NT Human topoisomerase I pseudogene 1 1908 7759 1.74 00E+00 5901905 NT Homo spaiens butyrophilin subfamlly 3, member A2 (BTN3A2), mRNA 1910 6896 11990 4.05 00E+00 BE018066. 1 EST_HHUMAN bb73f11y1.NIH_MGC_12 Homo sapiens cDNA clone IMAGE : 3048045 5' 1916 6902 11995 1. 47 O. OE+00 4809282 NT Homo sapiens histidine ammonia-lyase (HAL) mRNA 1916 6902 11996 1. 47 O. OE+00 4809282 NT Homo sapiens histidine ammonia-lyase (HAL) mRNA 1926 6912 12007 9. 5 O. OE+00 4826638 NT Homo sapiens actinin, alpha 4 (ACTN4) mRNA 1926 6912 12008 9. 5 O. OE+00 4826638 NT Homo sapiens actinin, alpha 4 (ACTN4) mRNA 1940692612023 1. 41 O. OE+00 M33782. 1 NT Human TFEB protein mRNA, partial cds 1940692612Q24 1. 41 O. OE+00 M33782. 1 NT Human TFEB protein mRNA, partial cds 1942692812025 1. 95 00E+00 AW193024. 1 ESTHUMAN xi69b01. x1 NCtCGAPPan1 Homo sapiens cDNA clone IMAGE : 2679913 3' 1942 6928 12026 1.95 00E+00 AW 193024. 1 EST_HUMAN x169b01.x1 NCl_CGAP_Pan1 Homo sapiens cDNA clone IMAGE : 2679913 3' Page 187 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Simllar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top HIt Acession SEQ ID SEQ ID Databas ID NO : Signas BLAST E No. Database Top Hit Descriptor NO: NO: Source Value 1943 6929 12027 8.45 00E+00 6912457 NT Homo sapiens calcineurin binding protein 1 (KIAA0330), mRNA 1943 6929 12028 8. 45 O. OE+00 6912457 NT Homo sapiens calcineurin binding protein 1 (KIAA0330), mRNA 1945 6931 12030 1. 25 O. OE+00 7662095 NT Homo sapiens KIAA0408 gene product (KIAA0408), mRNA 1946 6932 12031 1.88 00E+00 AB0111491. NT Homo sapiens mRNA for KIAA0577 protein, complete cds 1947 6933 12032 1. 43 O. OE+00 Z47556. 1 NT H. sapiens genes for semenogelin I and semenogelin 11 1947 6933 12033 1. 43 00E+00 Z37556.1 NT H. sapiens genes for semenogelin I and semenogelin 11 1954 694012042 3. 49 00E+00 AB040946. 1 NT Homo sapiens mRNA for KIAA1513 protein, partial cds 1971 6956 12060 0. 94 O. OE+00 AF273841. 1 NT Homo sapiens SMCY (SMCY) gene, complete cds 1971 6956 12061 0. 94 O. OE+00 AF273841. 1 NT Homo sapiens SMCY (SMCY) gene, complete cds 2000 6983 12087 1.1 00E+00 7706742 NT Homo sapiens TP53TG3a (TP53TG3a), mRNA 2004 6987 12091 13. 6 O. OE+00 BE743215. 1 EST_HUMAN 601573895F1 NIH_MGC_9 Homo sapiens cDNA clone IMAGE:3835 198 5' 2004 6987 12092 13. 6 O. OE+00 BE743215. 1 EST_HUMAN 601573895F1 NIH_MGC_9 Homo sapiens cDNA clone IMAGE:3835198 5' Homo sapiens coagulation factor IX (plasma thromboplastic component, Christmas disease, hemophilia B) 2006 698912093 1. 98 O. OE+00 4503648 NT (F9) mRNA 2007 6990 12094 1. 02 00E+00 BF207688. 1 EST_HUMAN 601861974F1 NIH_MGC_53 Homo sapiens cDNA clone IMAGE : 4081483 5' 2008 6991 12095 5. 35 O. OE+00 AU140831. 1 EST HUMAN. AU140831 PLACE4 Homo sapiens cDNA clone PLACE4000321 5' 2010 6993 12097 1. 29 O. OE+00 AA077589. 1 ESTHUMAN 7B22E10 Chromosome 7 Fetal Brain cDNA Library Homo sapiens cDNA clone 7B22E10 2010 6993 12098 1. 29 00E+00 AA077589. 1 EST_HUMAN 7B22E10 Chromosome 7 Fetal Brain cDNA Library Homo sapiens cDNA clone 7B22E10 2012 6995 2. 47 O. OE+00 7657468 NT Homo sapiens similar to rat integral membrane glycoportein POM121 (POM121L1), mRNA 2014 6997 1. 21 O. OE+00 4585863 NT Homo sapiens phosphodiesterase 6A, cGMP-specific, rod, alpha (PDE6A), mRNA 2015 6998 12101 1. 06 O. OE+00 Z42399. 1 EST HUMAN HSCOIC021 normalized infant brain cDNA Homo sapiens cDNA clone c-0ic02 qv90f09. x1 NCl_CGAP)Ut2 Homo sapiens cDNA clone IMAGE : 1988871 3'similar to-contains Alu repetitive 2017 7000 1. 43 00E+00 AI244247. 1 EST_HUMAN element ; 2021 7004121096. 29O. OE+00 BE877225. 1 EST_HUMAN 601485146F1 NIH_MGC_69 Homo sapiens cDNA clone IMAGE : 3887747 5' 2023 7006 12111 4. 71 O. OE+00 BF315325. 1 EST_HUMAN 601902604F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 4135320 5' 2023 7006 12112 4.71 00E+00 BF315325. 1 EST_HUMAN 60190264F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 4135320 5' 2028 7011 12118 2. 79 O. OE+00 BE697125. 1 EST_HUMAN RC3-CT0413-270700-022-d10 CT0413 Homo sapiens cDNA 2028 7011 12119 2. 79 O. OE+00 BE697125. 1 ESTHUMAN RC3-CT0413-270700-022-d10 CT0413 Homo sapiens cDNA 2033 7016 12125 2. 04 00E+00 L00620. 1 NT Human plasma membrane calcium ATPase isoform 2 (APT2B2) mRNA, comlete cds 2033 7016 12126 2. 04 00E+00 L00620. 1 NT Human plasma membrane calcium ATPase isoform 2 (APT2B2) mRNA, comlete cds 2036 701912129 1*7O. OE+00 4758489 NT Homo sapiens GTP binding protein 1 (GTPBP1) mRNA 2055 7037 2. 76 O. OE+00 BE767964. 1 ESTHUMAN QV1-GN0065-140800-318-c10GN0065 Homo sapiens cDNA 2056 7038 1.41 00E+00 AF018963. 1 NT Homo sapiens X-linked juvenile retinoschisis protein (XLRS1) gene, exon 6 and complete cds 2058 7040 12149 4.46 00E+00 BF027562. 1 EST HUMAN 601672066F1 NIH_MGC 20 Homo sapiens cDNA clone IMAGE : 3954785 5' Page 188 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Toe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top HIt Dexcriptor EQ ID SEQ ID NO: Signal BLASTE No. Soruce NO: NO: Vlue Vaque 20591 7041 12150 1. 77 O. 00E+00 BE072624. 1 EST HUMAN PMO-BT05470210300-004-F04 BT0547 Homo sapiens cDNA Homo sapiens glutathione S-transferase theta 2 (GSTT2) and glutathione S-transferase theta 1 (GSTT1) 2061 7043 12151 2. 11 00E+00 AF240786. 1 NT genes, complete cds 2062 7044 121523. 64 O. OE+00 AW752708. 1 EST_HUMAN IL3-CT0219-271099-022-G10 CT0219 Homo sapiens cDNA 2064 7046 12154 4. 62 O. OE+00 Ai904640. 1 EST HUMAN QV-BT065-020399-092 BT065 Homo sapiens cDNA 2064 7046 12155 4. 62 O. OE+00 Api904640. 1 EST HUMAN QV-BT065-020399-092 BT065 Homo sapiens DNA 2116 7096 1. 21 O. OE+00 L14787. 1 NT Human DNA-binding protein mRNA, 3 end 2122 7102 12214 1. 93 O. OE+00 BE274696. 1 EST-HUMAN 601122338FI NIH MGC 20 Homo sapiens cDNA clone IMAGE : 3346688 5' 2124 7104 12217 1. 01 O. OE+00 D87685. 1 NT Humen mRNA for KIAA0244 gene, partial cds 2125 7105 12218 42.14 00E+00 AV738288.1 EST_HUMAN AV738288 CB Homo sapiens cDNA clone CBNBDE08 5' 2125 7105 12219 42. 14 O. OE+00 AV738288. 1 EST HUMAN AV738288 CB Homo sapiens cDNA clone CBNBDE08 5' 2127 7107 12221 3. 7 O. OE+00 AA931691. 1 EST HUMAN 0032e01.s1. NCl_CGAP_Lu5 Homo sapiens cDnA clone IMAGE : 1567896 3' 2127 7107 12221 2129 7109 1.02 00E+00 M19828.1 NT Human apalipoprotin B-100 (apoB), gene, exons 22 through 29 2132 7112 12225 52.74 00E+00 BF34434.1 EST_HUMAN 602014829F1 NCl_CGAP Brn64 Homo sapiens cDNA clone IMAGE: 4150734 5; 2133 7113 12226 55. 46 O. OE+00 BE748899. 1 EST-HUMAN 601572186TI NIFF MGC 55 Homo sapiens dna 2136 7116 12229 4.45 00E+00 BF377897.1 EST_HUMAN CM1-TN01410250900-439-b08 TN0141 Homo sapiens cDNA 2136 7116 12230 4.45 O. OE+00 BF377897. 1 EST HUMAN CM1-TNO141-250900-439-b08 TN0141 Homo sapiens cDNA 2140 12235 4. 41 O. OE+00 BF313617. 1 EST HUMAN 601900261 F1 NIH MGC_19 Homo sapiens cDNA clone IMAGE : 4129622 5' bb84e.y1 NIH_MGC_10 Homo sapiens cDNA cione IMAGE : 3049082 5' similar to TR : Q15170 Q15170 2143 7122 12238 2.4 00E+00 BE018750.1 EST_HUMAN TRANSCRIPTION FACTOR S-II-RELATED PROTEIN; zk53c07.s1 Soares_pregnat_uterus_NbHPU Homo sapiens cDNA clone IMAGE : 486540 3'similar to 2145 7124 12240 2. 17 O. OE+00 AA042813. 1 EST HUMAIN gb : X65857 cds1 OLFACTORY RECEPTOR-LIKE PROTEIN HGMP07E (HUMAN) ; I zk53c07. s1 Soares_pregnant_uterus_NbHPU Homo sapiens cDNA clone IMAGE : 486540 3'similar to 2145 7124 12241 2. 17 O. 00E+00 AA042813. 1 EST HUMAN gb : X65857 cds1 OLFACTORY RECEPTOR-LIKE PROTEIN HGMP07E (HUMAN) ; 2153 7132 12249 2. 86 O. OE+00 AL163204. 2 NT Homo sapiens chromosome 21 segment HS21 C004 2153 7132 12250 2. 86 O. OE+00 Al163204.2 NT sapiens chromosome 21 segment HS21 C004 2154 Homo O. 7133 12251 2.34 7662401 NT Homo sapiens 00E+00 protein KIAA0952 2154 7133 (KIAA0952), mRNA O. OE+00 7662401 NT Homo sapiens 12252 2.34 protein KIAA0952 2159 7138 2. 31 O. OE+00 U36264. 1 NT Human (KIAA0952), mRNA gene, exon 16 beta-prime-adaptin (BAM22) 43 2160 7139 12257 1. 00E+00 AA282281. 1 EST HUMAN zt12b10.r1 NCl_CGAP-GCB1 Homo sapiens cDNA clone IMAGE : 712891 5' 2167 7146 12263 2.22 00E+00 BE897487.1 EST_HUMAN 6013323F1NIH_MGC_72 Homo sapiens cDNA clone IMAGE:3917453 5' 2180, 7159 12279 8. 49 O. OE+00 4557556 NT Homo sapiens E1A binding protein p300 (EP300) mRNA 2185 7164 12284 1.93 O. 00E+00 7662401 NT Homo sapiens KIAA0952 protein (KIAA0952), mRNA 2191 7170 12291 6.09 00E+00 BE9895281.1 EST_HUMAN 601433525F1 NIH_MGC_72 Homo sapiens cDNA clone IMAGE:3918607 5' Page 189 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession To Hit SEQ ID SEQ ID ID NOP Signai BLAST E No. Source NO: NO: Saure Value 2196 7174 12295 2.37 00E+00 BE905563.1 EST_HUMAN 601494208F1 NIH_MGC_70 Homo sapiens cDNA clone MAGE:3897457 5' 2195 12296 2.37 0. 7174 BE905563. OE+00 cDNA clone IMAGE 1 EST-HUMAN 6014952OBF1 NIH-MGC 70 Homo sapiens KIAA1363 : 3897457 5' 2197 7175 12298 3. 61 O. OE+00 AB037784. 1 NT Homo sapiens mRNA for 2237 7214 12331 3.29 00E+00 11545747 NT Homo protein, partial cds 27 0. sapiens differen6ally expressed in FDCP (mouse homolog) 6 (DEF6), MARNA 2237 7214 12332 3. 29 O. OE+00 11545748 NT Homo sapiens differentially expressed in FDCP (mouse homolog) 6 (DEF6), MARNA 2238 7215 12333 2. cDNA clone IMAGE OE+00 A1076404. 1 EST HUMAN oz09c07. x1 Soares fetal liver spleen_1NFLS S1 Homo sapiens EST_HUMAN zv78a11.r1 Soares_total_fetus_Nb2HF8_9w : 1674828 3' 2241 7218 12336 3. 84 O. OE+00 AA429001. 1 cDNA clone IMAGE Homo sapiens 12337 : 759740 5' 2241 7218 EST_HUMAN zv78a11.r1 Soares_total_fetus_Nb2HF8_9w Homo sapiens cDNA clone IMAGE 3. 84 O. OE+00 AA429001. 1 BF347039. : 759740 5' 2243 7220 12339 3. 72 O. OE+00 EST_HUMAN 602021846F1 NCl_CGAP_Brn67 1 cDNA clone IMAGE Homo sapiens 7f22a02.x1 NCl_CGAP_CLL1 : 4157339 5' 2248 7225 12345 1. 02 O. OE+00 6325466 NT Homo sapiens flavin containing monooxygenase 3 (FM03), mRNA cDNA clone IMAGE Homo sapiens 12351 2.79 00E+00 : 3295370 3'similar to TR : 094939 094939 2255 7232 EST_HUMAN BE676095. 1 KIAA0867 PRO TEIN ; 2258 7235 12353 16. 03 O. OE+00 AF044571. 1 NT Homo sapiens phosphorylase kinase alpha subunit (PHKA2) gene, exon 32 I 2259 7236 12354 1. 78 O. OE+00 AI625542. 1 EST_HUMAN ty57c08. x1 NCI_CGAP Ut2 Homo sapiens cDNA clone IMAGE : 2283182 3'I 2261 7238 12355 1. 15 O. OE+00 AB011399.1 NT Homo sapiens gene for AF-6, complete cds 2264 7241 12357 3. 07 O. OE+00 7662401 NT Homo sapiens KIAA0952 protein (KAA0952), mRNA 2264 7241 12358 3. 07 O. OE+00 7662401 NT Homo sapiens KIAA0952 protein (KIAA0952), mRNA 2267 7244 12361 3. 48 O. OE+00 5803178 NT Homo sapiens sperm specific antigen 2 (SSFA2), MARNA' 2267 7244 12362 3. 48 O. OE+00 5803178 NT Homo sapiens sperm specific antigen 2 (SSFA2), MARNA 2273 7249 12365 2. 22 O. OE+00 7662007 NT Homo sapiens KIAA0218 gene product (KIAA0218), mRNA 2273 7249 12366 2. 22 O. OE+00 7662007 NT Homo sapiens KIAA0218 gene product (KIAA0218), mRNA 1 NT Human mRNA 2277 7253 12371 1. 4 O. OE+00 D83778. KIAA0194 for NT Human mRNA for KIAA0194 gene, partial cds 2277 7253 123721. 4O. OE+00 D83778. 1 partial cds gene, beta. 2287 7263 12380 2. 25 O. OE+00 5174678 NT Homo sapiens signal regulatory protein, (SIRP-BETA-1) mRNA 1 2291 7266 12384 3.96 0. EST_HUMAN AU131142 NT2RP3 Homo sapiens cDNA clone NT2RP3002064 5' OE+00 AU131142. 1 7267 47.81 00E+00 2292 BE794026. 1 EST_HUMAN 601586843F1 NIH_MGC_7 cDNA clone IMAGE Homo sapiens 12385 2.44 0. : 3941003 5' 2293 7268 EST_HUMAN MR10SN0033-120400-022-a04 SN0033 OE+00 AW867076. 1 2294 7269 12386 4.95 00E+00 Homo sapiens cDNA KIAA0244 7662017 NT Homo sapiens (KIAA0244), mRNA protein 2295 7170 12387 2.03 0 00E+00 (H6PD), mRNA 4758497 NT Homo sapiens hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) 2295 7270 12388 2.03 0. partial OE+00 4758497 NT Homo sapiens hexose-6-phosphate dehydrogenase (glucose 1-dehydrogenase) (H6PD), mRNA Homo sapiens cytochrome P450 polypeptide 43 (CYP3A43) gene, NT cds ; cytochrome P450 polypepbde 4 (CYP3A4) and cytochrome P450 pdypepbde 7 (CYP3A7) genes, compete cds ; and cytochrome P450 2296 7271 4. 1 O. OE+00 AF280107. 1 polymeptide 5 (CYP3A5) gene, partial cds 2298 7273 12390 21. 5 00E+00 AU118082. 1 EST_HUMAN AU118082 HEMBA1 Homo sapiens cDNA clone HEMBA1002839 S' Page 190 of 209 Table 4 Single Exon Probes Expressed in HBL100 Celis Probe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source NO: NO: Source Value 2298 7273 12391 21.5 0.1E+00 AU118082.1 EST_HUMAN AU118082 HEMBA1 Homo sapiens cDNA clond HEMBA 1002839 5' 2298 7273 12392 21. 5 O. OE+00 AU118082. 1 EST HUMAN AU118081 HEMBA1 Homo sapiens cDNA clone HEMBA1002839 5' 2299 7274 12393 1. 24 O. OE+00 8923089 NT Homo sapiens hypothetical protein FLJ20081 (FLJ20081), mRNA 2316 7291 1.51 0.0E+00 BE814424. 1 EST_HUMAN MR0-BN0070-090600-029-d12 BN0070 Homo sapiens cDNA 2348 7322 12441 0. 99 O. OE+00 AU119582. 1 EST_HUMAN AU119582 HEMBA1 Homo sapiens cDNA clone HEMBA1006155 5' ox60b02. x1 Soares NhHMPu S1 Homo sapiens cDNA clon IMAGE : 1660683 3' similar to TR : 008662 2349 7323 3.28 0.0E+00 AI042035. 1 EST HUMAIN 008662 230KDA PHOSPHATIDYLINOSITOL 4-KINASE. ; 2350 7324 12442 1.18 0.0E+00 8923620 NT Homo sapiens hypothetical protein FLJ20693 (FLJ20693), mRNA 2354 7328 4. 15 O. OE+00 BE895605. 1 EST-HUMAN 601432608FI NIH MGC 72 Homo sapiens cDNA clone IMAGE : 3918168 5' 2365 7339 3. 39 O. OE+00 AB005622. 1 EST HUMAN AB005622 HeLa cDNA (T. Noma) Homo sapiens cDNA similar to adenate kinase isozyme 2 2369 7342 14260 5.53 0.0E+00 6006002 NT Homo sapiens glutamate receptor, ionotropic, N-ethyl D-aspartate 2A (GRIN2A) mRNA 2372 7344 12463 1.57 0.0E+00 D85606.1 NT Homo sapiens gene for cholecystokinin type-A receptor, complete cds 2372 7344 12464 1. 57 O. OE+00 D85606. 1 NT Homo sapiens gene for cholecystokinin type-A receptor, complete cds 2380 7352 12473, 1. 34 O. OE+00 AF106275. 1 NT Homo sapiens immunoglobulin-like transcript 1c variant 4 (ILT1c gene, taxon 6 2384 7355 124771 0. 95 O. OE+00 BF345274. 1 EST_HUMAN 602018058F1 NCI_CGAP_Brn67 Homo sapiens cDNA clone IMAGE : 4153670 5' 2390 7361 12484 6. 99 O. OE+00 5729777 NT Homo sapiens collagen, type XII, alpha 1 (COL12A1), mRNA 2398 7369 12490 16.53 0. OE+00 BF569144. 1 EST_HUMAN 602184558T1 NIH_MGC_42 Homo sapiens cDNA clone IMAGE : 4300383 3' 2407 7378 12497 2. 46 0.0E+00 AW166922. 1 EST_HUMAN ha04h0.4.x1 NCI_CGAP_Kid12 Homo sapiens cDNA clone IMAGE : 2872759 3' 2409 7380 12498 2. 09 O. OE+00 AW501010. 1 EST_HUMAN UI-HF-BP0p-ais-c-07-0-UI.r1 NIH_MGC_51 Homo sapiens cDNA clone IMAGE : 3072780 5' 2433 7404 2. 13 O. OE+00 AW813853. 1 EST_HUMAN RC3-ST0197-300300-016-c04 ST0197 Homo sapiens DNA 2438 7409 12526 27.9 0.0E+00 BE795542. 1 EST HUMAIN 601592530F1 NIH_MGC_7 Homo sapiens cDNA clone IMAGE : 3946518 5' 2439 6887 11981 1. 18 O. OE+00 7657038 NT Homo sapiens death receptor 6 (DR6), mRNA 2440 7410 12527 1. 33 O. OE+00 BF509482. 1 EST HUMAN UI-H-Bi4-aoz-b-08-0-UI. s1 NCI CGAP Sub8 Homo sapiens cDNA clone IMAGE : 3086535 3' 2443 7413 12529 3. 32 O. OE+00 Z32684.2 NT Homo sapiens mRNA for membrane transport protein (XK gene) 2445 7415 3. 21 O. OE+00 5453871 NT Homo sapiens platelet-derived growth factor receptor-like (PDGFRL) mRNA 2448 7418 12533 1. 05 O. OE+00 BE910378. 1 EST_HUMAN 601503356F1 NIH_MGC_70 Homo sapiens cDNA clone IMAGE : 3905148 5' 2449 7419 12534, 2. 35 O. OE+00 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM121 (POM121L1), mRNA 2450, 7420 12535 0.91 0. OE+00 BE150865. 1 EST_HUMAN RC4-HT0276-160200-013-d05 HT0276 Homo sapiens cDNA 2451 7421 12536 3. 02 O. OE+00 U93239.1 NT Human Sec62 (Sec62) mRNA, complete cds 2457 7427 12542 2. 2 O. OE+00 BE886490. 1 EST_HUMAN 601508211F1 NIH_MGC_71 Homo sapiens cDNA clone IMAGE : 3909866 5' 2461 7430 12547 4. 05 O. OE+00 BE875511. 1 EST_HUMAN 601489241F1 NIH_MGC_69 Homo sapiens cDNA clone IMAGE : 3891371 5' 2461 7430 12548 4.05 0.0E+00 BE875511.1 EST_HUMAN 601489241F1 NIH_MGC_69 Homo sapiens cDNA clone IMAGE : 3891371 5' 2462 7431 12549 1. 27 O. OE+00 AF114027. 1 EST_HUMAN AF114027 Homo sapiens lung fetus Homo sapiens cDNA clone ESF6 2464 7433 12552 1 O. 0E+00 AF245505. 1 NT Homo sapiens adlican mRNA, complete cds Page 191 of 209 Table 4 Sinale Exon Prohes ExnrPS. sPrl in HRI 1f1 (1 Callc Most Similar Prob Exon Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit ID NO : Signas BLAST E No. Source NO: NO: Source Value 2480 7449 12560 1.91 0.0E+00 BE536921.1 EST_HUMAN 601064738F1 NIH_MGC_10 Homo sapiens cDNA clone IMAGE:3451161 5' 2485 7453 125671 3. 77 O. OE+00 AU143277. 1 EST HUMAN AU143277 Y79AA1 Homo sapiens cDNA clone Y79AA1001673 5' 2485 7453 12568 3. 77 O. OE+00 AU143277. 1 EST HUMAN AU143277Y79AA1 Homo sapiens cDNA clone Y79AA1001673 5' 2486 7454 125691 3. 86 O. OE+00 BE292896. 1 EST HUMAN 601105312F1 NIH MGC 15 Homo sapiens cDNA clone IMAGE : 2987955 5' 2486 7454 12570 3.86 0. OE+00 BE292896. 1 EST HUMAN 601105312F1 NIH MGC 15 Homo sapiens cDNA clone IMAGE : 2987955 5' 7q27h12.x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 3'similar to TR : 000246 000246 2487 7455 125711 0. 931 O. OE+00 BF223041. 1 EST-HUMAN HYPOTHETICAL 9. 3 KD PROTEIN ; 2490 7458 12573 5. 96 O. OE+00 AF245505. 1 NT Homo sapiens adlican mRNA, complete cds 2508 7476 12591 0. 91 O. OE+00 BE296613. 1 EST_HUMAN 601173631F1 NIH_MGC_17 Homo sapiens cDNA clone IMAGE : 3529159 5' 2522 7708 12608 1.49 0. OE+00 AB037836.1 NT Homo sapiens mRNA for KIAA1415 protein, partial cds 2522 7708 12609 1. 49 O. OE+00 AB037836. 1 NT Homo sapiens mRNA for KIAA1415 protein, partial cds 2523 7489 1. 95 O. OE+00 BF513835. 1 EST_HUMAN UI-H-BW1-amp-f-12-0-UI.s1 NCI_CGAP_Sub7 Homo sapiens cDNA clone IMAGE : 3070631 3' 2528 7494 12614, 1. 25 O. OE+00 BF672818.1 EST_HUMAN 602152653F1 NIH_MGC_81 Homo sapiens cDNA clone IMAGE : 4293612 5' 2530 7496 1. 1 O. OE+00 BE616695. 1 EST HUMAN 601279873F1 NIH MGC 39 Homo sapiens cDNA clone IMAGE : 3621786 5' 2539 7504 12623 2. 22 O. OE+00 AB037742. 1 NT Homo sapiens mRNA for KIAA1321 protein, partial cds Homo sapiens TATA box binding protein (TBP)-associated factor, RNA polymerase II, I, 28kD (TAF21) 2540 7505 12624 2. 36 O. OE+00 5032150 NT mRNA 2542 7507 12626 5. 06 O. OE+00 AB037859. 1 NT Homo sapiens mRNA for KIAA1438 protein, partial cds 2543 7508 12627 1. 42 0.0E+00 BE795445. 1 EST_HUMAN 601590108F1 NIH_MGC_7 Homo sapiens cDNA clone IMAGE : 3944304 5' 2543 7508 12628 1. 42 O. OE+00 BE795445. 1 EST HUMAN 601590108F1 NIH MGC 7 Homo sapiens cDNA clone IMAGE : 3944304 5' 2546 7511 12629 1. 35 O. OE+00 BE293328. 1 EST HUMAN 601143722F1 NIH MGC 15 Homo sapiens cDNA clone IMAGE : 3051389 5' 2553 7518 6. 42 O. OE+00 BE792472. 1 EST HUMAN 601584930F1 NIH_MGC_7 Homo sapiens cDNA clone IMAGE : 3939222 5' 2555 7520 12637 1. 06 O. OE+00 AB020710. 1 NT Homo sapiens mRNA for KIAA0903 protein, partial cds 2563 7527 12645 2.7 0.0E+00 4504686 NT Homo sapiens IMP (inosine monophosphate) dehydrogenase 1 (IMPDH1) mRNA 2574 7537 12653 4 0. OE+00 AF173227. 1 NT Homo sapiens guanylate cyclase-activating protein 2 (GUCA1B) gene, exon 1 2577 7540 12654 1. 3 0.0E+00 AB011108.1 NT Homo sapiens mRNA for KIAA0536 protein, partial cds 2580 7543 12657 1. 88 O. OE+00 AU133385. 1EST-HUMAN AU133385 NT2RP4 Homo sapiens cDNA clone NT2RP4001964 5' 2581 7544 12658 1. 75 O. OE+00 M69225. 1 NT Human bullous pemphigoid antigen (BPAG1) mRNA, complete cds 2584 7547 12661 2. 23 O. OE+00 AU130403. 1ESTHUMAN AU130403NT2RP3 Homo sapiens cDNA clone NT2RP3000779 5' 2584 7547 12662 2. 23 O. OE+00 AU130403. 1 EST HUMAN AU130403 NT2RP3 Homo sapiens cDNA clone NT2RP3000779 5' 2587 7550 12665 1.36 0. OE+00 AW887015. 1 EST HUMAN RC1-OT0086-220300-011-d07 OT0086 Homo sapiens cDNA 2590 7553 12668 1.01 0.0E+00 BF000018. 1 EST HUMAN 7h15h05. x1 NCI CGAP_Co16 Homo sapiens cDNA clone IMAGE : 3316089 3' 2591 7554 12669 5.22 0.0E+00 BE383165.1 EST_HUMAN 601298714F1 NIH_MGC_19 Homo sapiens cDNA clone IMAGE : 3628923 5' 2592 7555 8.33 0.0E+00 BE531263.1 EST_HUMAN 601278373F1 NIH_MGC_39 Homo sapiens cDNA clone IMAGE : 3610267 5' Page 192 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Prob Exon Top Hit SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit ID NO : Signal BLAST E No. source NO: NO: Source Value 2616 7578 12690 1.38 0.0E+00 8922843 NT Homo sapiens hypothetical protein FLJ11052 9FLJ11052), mRNA 2626 7586 12698 1.22 0. OE+00 AB037732. 1 NT Homo sapiens mRNA for KIAA1311 protein, partial cds EST188414 HCC cell line (matastasis to liver in mouse) II Homo sapiens cDNA 5'end similar to ribosomal 2650 7610 27. 94 O. OE+00 AA316723. 1 EST HUMAIN protein L29 2651 7611 12721 1. 31 O. OE+00 BE794884. 1 EST_HUMAN 601589625F1 NIH_MGC_7 Homo sapiens cDNA clone IMAGE : 3943591 5' 2655 7615 12726 5. 13 O. OE+00 U36253. 1 NT Human beta-prime-adaptin (BAM22) gene, exon 5 2656 7616 12727 5.03 0. OE+00 7669517 NT Homo sapiens neuregulin 1 (NRG1), transcript variant SMDF, mRNA 2657 7617 12728 2. 44 O. OE+00 AF110763. 1 NT Homo sapiens skeletal muscle LIM-protein 1 (FHL1) gene, complete cds 2658 7618 12729 1. 27 O. OE+00 AB051826. 1 NT Homo sapiens hG28K mRNA for GTP-binding protein like 1, complete cds 2664 7623 12735. 26. 29 O. OE+00 BE796376. 1 EST_HUMAN 601591991F1 NIH_MGC_7 Homo sapiens cDNA clone IMAGE : 3945983 5' 26657624 12736 1. 5 O. OE+00 BF680632. 1 EST_HUMAN 602155923F1 NIH_MGC_83 Homo sapiens cDNA clone IMAGE : 4297132 5' 2668 7778 12740 24. 53 O. OE+00 BE563433. 1 EST_HUMAN 601335485F1 NIH_MGC_39 Homo sapiens cDNA clone IMAGE : 3689564 5' 2669 7627 2.28 0. OE+00 AV721647. 1 EST_HUMAN AV721647 HTB Homo sapiens cDNA clone HTBBYE09 5' 2671 7629 12743 2. 82 O. OE+00 5174486 NT Homo sapiens spermatogenesis associated PD1(KIAA0757) mRNA 2671 7629 12744 2.82 0.0E+00 5174486 NT Homo sapiens spermatogenesis associated PD1 (KIAA0757) mRNA 2672 7630 12745 1.27 0. OE+00 8923441 NT Homo sapiens hypothefical protein FLJ20477 (FLJ20477), mRNA 2672 7630 12746 1. 27 O. OE+00 8923441 NT Homo sapiens hypothetical protein FLJ20477 (FLJ20477), mRNA 2673 7631 12747 2.26 0. OE+00 AF290195. 1 NT Homo sapiens hypertension-related calcium-regulated gene mRNA, complete cds 2674 7632 51.51 O. 0E+00 AV651066. 1 EST_HUMAN AV651086 GLC Homo sapiens cDNA clone GLCCLD07 3' 2675 7633 12748 3. 33 O. OE+00 BF377897. 1 EST_HUMAN CM1-TN0141-250900-439-b08 TN0141 Homo sapiens cDNA 2675 7633 12749 3. 33 O. OE+00 BF377897. 1 EST_HUMAN CM1-TN0141-250900-439-b08 TN0141 Homo sapiens cDNA 2682 7640 12756 19. 56 0.0E+00 BE747193. 1 EST_HUMAN 601580903F1 NIH_MGC_9 Homo sapiens cDNA clone IMAGE : 3929472 5' 2687 7645 12760 3. 09 O. OE+00 BF037713. 1 EST_HUMAN 601462038F1 NIH_MGC_66 Homo sapiens cDNA clone IMAGE : 3865497 5' 2695 7653 0. 93 O. OE+00 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21 C001 2696, 7654 12768, 2. 53 O. OE+00 BF514110. 1 EST_HUMAN UI-H-BW1-amw-e-07-0-UI.s1 NCI_CGAP_Sub7 Homo sapiens cDNA clone IMAGE : 3071340 3' 2703 7660 2.14 0.0E+00 4503098 NT Homo sapiens chondroition sulfate proteoglycan 4 (melanoma-associated) (CSPG4), MARNA 2708 7665 12776 1. 05 O. OE+00 7705275 NT Homo sapiens angiopoietin-3 (ANG-3), MARNA 2708 7665 12777 1. 05 O. OE+00 7705275 NT Homo sapiens angiopoietin-3 (ANG-3), mRNA 27097666 12778 2. 68 0.0E+00 BF677694, 1 EST_HUMAN 602085579F1 NIH_MGC_83 Homo sapiens cDNA clone IMAGE : 4249915 5' 2715 7672 12786 1. 43 O. OE+00 7427522 NT Homo sapiens protein tyrosine phosphatase, receptor type, T (PTPRT), mRNA 2719 7676 12789 26.73 0.0E+00 AV725534. 1 EST_HUMAN AV725534 HTC Homo sapiens cDNA clone HTCCCA03 5' 2719 7676 12790 26.73 0.0E+00 AV725534. 1 EST_HUMAN AV725534 HTC Homo sapiens cDNA clone HTCCCA03 5' au55d0.4.y1 Schneider fetal brain 00004 Homo sapiens cDNA clone IMAGE : 2518663 5' smiliar to 2721 7678 11.75 0.0E+00 AI879163.1 EST_HUMAN SW : R13A_HUMAN P40429 60S RIBOSOMAL PROTEIN L13A ; Page 193 of 209 Tabie 4 Single Exon Probes Expressed in HBL100 Celis Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Eression (Top) Hit Top Hit Acession pabase Top Hit Descriptor NO : NO : NO : Signal BLAST E N Source, NO : NO :.,, Source Value 2724 7681 12795 2. 71 O. OE+00 BF530661. 1 EST HUMAN 602071957F1 NCI CGAP Brn67 Homo sapiens cDNA clone IMAGE : 4214679 5' 2725 7682 12796 58. 63 O. OE+00 BE872768. 1 EST HUMAN 601450912F1 NIH_MGC 65 Homo sapiens cDNA clone IMAGE : 3854642 5' 2727 7684 12797 2. 2 O. OE+00 AU131494. 1 EST_HUMAN AU131494 NT2RP3 Homo sapiens cDNA clone NT2RP3002672 5' 2727 7684 12798 2. 2 O. OE+00 AU131494. 1 EST HUMAN AU131494 NT2RP3 Homo sapiens cDNA clone NT2RP3002672 5' 2728 7685 12799 68. 47 O. OE+00 BE300344. 1 EST HUMAN 600944794F1 NIH MGC 17 Homo sapiens cDNA clone IMAGE : 2960806 5' 2728 7685 12800 68. 47 O. OE+00 BE300344. 1 EST HUMAN 600944794F1 NIH MGC 17 Homo sapiens cDNA clone iMAGE : 2960806 5' 2734 5251 10262 2. 82 O, OE+00 S76830. 1 NT glycoProtein D=Duffy group an8gen [human, blood, Genomic DNA, 3068 nt] 2737 7692 3. 43 O. OE+00 AB033281. 1 NT Homo sapiens BTRCP2 mRNA for F-box and W D-repeats protein isoform C, complete cds 2743 5747 10768 1. 92 O. OE+00 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds 2743 5747 10769 1. 92 O. OE+00 AF264750. 1 NT Homo sapiens ALR-like protein mRNA, partial cds Homo sapiens cytochrome P450, subfamily I (diopn-inducible), polypeptide 1 (glaucoma 3, primary infan6le) 2747 6032 11062 2. 78 O. OE+00 4503202 NT (CYP1B1) mRNA Homo sapiens cytochrome P450, subfamily I (dioan-inducible), polypeptide 1 (glaucoma 3, primary infan6le) 2747 6032 11063 2. 78 O. OE+00 4503202 NT (CYP1B1) mRNA 2762 7783 12808 5. 17 O. OE+00 X85980. 1 NT H. sapiens serine hydroxymethyltransferase pseudogene 2763 7784 1. 34 O. OE+00 AF068624. 1 NT Homo sapiens 5-amindewlinata synthase 2 (ALAS2) gene, complete cds 2765 7786 1. 35 O. OE+00 AB040960. 1 NT Homo sapiens mRNA for KIAA1527 protein, partial cds Homo sapiens partial rpl3 gene for ribosomal protein L3, U82 snoRNA, U83a snoRNA and U83b snoRNA 1. 16 O. OE+00 AJ238852. 1 NT genes 2772 7793 12813 2. 35 O. OE+00 AL163201. 2 NT Homo sapiens chromosome 21 segment HS21 C001 2775 7796 12815 1. 41 O. OE+00 M91803. 1 NT Human sodium channel mRNA 2777 77g8 12817 1. 6 O. OE+00 M80902. 1 NT Human AHNAK nucleoprotein mRNA, 5'end 2781 7802 1. 42 O. OE+00 X73428. 1 NT H. sapiens Id3 gene for HLH type transcrip6on factor 2783 7804 2. 78 O. OE+00 AL163268. 2 NT Homo sapiens chromosoma 21 segment HS21 C068 2784 7805 12821 1. 41 O. OE+00 7019584 NT Homo sapiens zinc finger protein 221 (ZNF221), mRNA 2784 7805 12822 1. 41 O. OE+00 7019584 NT Homo sapiens zinc finger protein 221 (ZNF221), mRNA 2784 7805 12823 1. 41 O. OE+00 7019584 NT Homo sapiens anc finger protein 221 (ZNF221), mRNA 27g7 7808 12825 0. 96 O. OE+00 M98478. 1 NT Human transglutaminase mRNA, complete cds Homo sapiens gammma-cytoplasmic actin (ACTGP3) pseudogene 2792 7812 12829 36. 19 O. OE+00 D50657. 1 NT 27g2 7g12 12830 36. 19 O. OE+00 D50657. 1 NT Homo sapiens gammma-cytoplasmic actin (ACTGP3) pseudogene 2795 7815 12833 4. 89 O. OE+00 AL096857. 1 NT Novel human mRNA from chromosome 1, which has similarities to BAT2 genas 2796 7816 6. 12 O. OE+00 Y10658. 1 NT H. sapiens mRNA for nuclear DNA helicase II g 1. 14 O. OE+00 AF152303. 1 NT Homo sapiens protocadherin alpha C1 (PCDH-alpha-C1) mRNA, complete cds 27g8 7818 12834 71. 64 O. OE+00 4503470 NT Homo sapiens eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) mRNA Page 194 of 209 Table 4 Sinale Exon Probes Exnressed in HBL100 Cells Most Similar Porbe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO : Signal BLAST E No. Source NO: NO: Source Value 2796 7818 12835 71.64 0.0E+00 4503470 NT Homo sapiens eukaryotic transiation elongation factor 1 alpha 1 (EEF1A1) mRNA 2810 7830 12846 2. 53 O. 0E+00 2507280 NT Homo sapiens serine/threonine kinase 9 (STK9) mRNA 2813 7833 12850 1 O. OE+00 AL047599. 1 EST_HUMAN DKFZp586G0621_r1 586 (synonym : hute1) Homo sapiens cDNA clone DKFZp586G0621 2814 7834 2.12 0. 0E+00 4503098 NT Homo sapiens chondroitin sulfate proteoglycan 4 (melanoma-associated) (CSPG4), mRNA 2817 7837 12852 5. 88 O. OE+00 BE081896. 1 EST_HUMAN QV2-BT0636-130400-138-h03 BT0636 Homo sapiens cDNA 2817 7837 12853 5. 88 O. OE+00 BE081896. 1 EST_HUMAN QV2-BT0636-130400-138-h03 BT0636 Homo sapiens cDNA 2822 7843 12861 0. 85 O. OE+00 6806918 NT Homo sapiens low density lipoprotein-related protein 2 (LRP2), mRNA 2822 7843 12862 0. 85 O. OE+00 6806918 NT Homo sapiens OW density lipoprotein-related protein 2 (LRP2), mRNA 2825 7846 12866 3. 1 O. OE+00 AL163206. 2 NT Homo sapiens chromosome 21 segment HS21 C006 2825 7846 12867 3. 1 O. OE+00 AL163206. 2 NT Homo sapiens chromosome 21 segment HS21 C006 z96b11. s1 NCI_CGAP GCB1 Homo sapiens cDNA clone IMAGE : 683517 3' similar to contains Alu 2826 7847 12868 1. 03 O. OE+00 AA215579. 1 EST HUMAN repetitive element ; 2834 7854 4. 09 O. OE+00 Y19210. 1 NT Homo sapiens hHb5 gene for hair keratin, exons 1 to 9 2836 7856 12875 1. 08 O. 0E+00 4758279 NT Homo sapiens EphA4 (EPHA4) mRNA 2837 7857 12876 50. 46 O. OE+00 4503470 NT Homo sapiens eukaryotic transiation elongation factor 1 alpha 1 (EEF1A1) mRNA tn18d07.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE : 2167981 3'similar to TR : 016247 2838 7858 12877 2. 63 O. OE+00 AI561002. 1 EST HUMAIN 016247 F44E7. 2 PROTEIN. ; tn18d07.x1 NCI_CGAP_Brn25 Homo sapiens cDNA clone IMAGE : 2167981 3'similar to TR : 016247 2838 7858 12878 2. 63 O. OE+00 Api561002. 1 EST_HUMAN 016247 F44E7. 2 PROTEIN. ; 2840 7860 12880 1. 19 O. OE+00 p52740 swissprot zinc finger PROTEIN 132 2841 7861 12881 1. 05 O. OE+00 AF152338. 1 NT Homo sapiens protocadherin gamma C4 (PCDH-gamma-C4) mRNA, complete cds 2857 7877 12893 2. 2 O. OE+00 AB033093. 1 NT Homo sapiens mRNA for KIAA1267 protein, partial cds 2857 7877 12894 2.2 0.0E+00 AB033093.1 NT Homo sapiens mRNA for KIAA1267 protein, partial cds 2858 7878 12895 5. 42 O. OE+00 AB040941. 1 NT Homo sapiens mRNA for KIAA1508 protein, partial cds 2858 7878 12896 5. 42 O. OE+00 AB040941. 1 NT Homo sapiens mRNA for KIAA1508 protein, partial cds 2861 7881 12899 2. 79 O. 0E+00 7661903 NT Homo sapiens KIAAOIO0 gene product (KIAA0100), mRNA 2861 7881 12900 2. 79 O. 0E+00 7661903 NT Homo sapiens KIAA0100 gene product (KIAA0100), mRNA Homo sapiens myelddbymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog) ; translocated to, 4 2862 7882 12901 3. 2 O. 0E+00 5174574 NT (MLLT4) MARNA Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog) ; translocated to, 4 2862 7882 12902 3. 2 O. 0E+00 5174574 NT (MLLT4) mRNA 7n40d03.x1 NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3567028 3'similar to TR : Q9VLN1 2867 7886 12906 0.99 O. 0E+00 BF110702. 1 EST HUMAN Q9VLN1 CG17293 PROTEIN. ; Page 195 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Value 7n40d03.x1 NCI CGAP Lu24 Homo sapiens cDNA clone IMAGE : 3567028 3'similar to TR : Q9VLN1 2867 7886 12907 0.99 0.0E+00 BF110702. 1 EST HUMAN Q9VLN1 CG17293 PROTEIN.; 2875 7894 12917 2.17 0.0E+00 4505084 NT Homo sapiens melanoma antigen, family B, 4 (MAGE4), mRNA 2875 7894 12918 2. 17 O. OE+00 4505084 NT Homo sapiens melanoma antigen, family B, 4 (MAGE4), MARNA 2882 790112923 1. 65 O. OE+00 4758827 NT Homo sapiens neurexin III (NRXN3) mRNA 2883 7902 1.14 0. OE+00 X98494. 1 NT H.sapiens mRNA for M phase phosphoprotein 10 2886 790512926 0. 93 0.0E+00 AB033034. 1 NT Homo sapiens mRNA for KIAA1208 protein, partial cds 2888 7907 12927 0. 94 O. OE+00 X15309. 1 NT H. sapiens NF-H gene, exon 4 2888 7907 12928 0. 94 O. OE+00 X15309. 1 NT H. sapiens NF-H gene, exon 4 2890 790912930 8. 37 O. OE+00 AF106275. 1 NT Homo sapiens immunoglobulin-like transcript 1c variant 4 (ILT1c) gene, exon 6 2904 7923 1.38 0. OE+00 AI149880. 1 EST_HUMAN qf43f09.x1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE : 1752809 3' 2912 7931 12950 1. 21 0.0E+00 4506118 NT Homo sapiens prospero-related homeobox 1 (PROX1) mRNA 2913 7932 12951 4. 29 O. OE+00 AB004884. 1 NT Homo sapiens mRNA for PKU-alpha, partial cds 2924 7943 12959 2. 03 O. OE+00 7662273 NT Homo sapiens KIAA0737 gene product (KIAA0737), mRNA 2925 7944 12960 2. 05 O. OE+00 5729755 NT Homo sapiens calcium channel, voltage-dependent, gamma subunit 3 (CACNG3), mRNA 2925 7944 12961 2.05 0.0E+00 5729755 NT Homo sapiens calcium channel, voltage-dependent, gamma subunit 3 (CACNG3), mRNA 2935 7954 12971 1. 3 O. OE+00 AF114488. 1 NT Homo sapiens intersectin short isoform (ITSN) mRNA, complete cds 2935 7954 12972, 1. 3 O. OE+00 AF114488. 1 NT Homo sapiens intersectin short isoform (ITSN) mRNA, complete cds 2958 7977 12992 1. 23 O. OE+00 M74099. 1 NT Human displacement protein (CCAAT) mRNA 2967, 7985 12999 0. 84 O. OE+00 AW976266. 1 EST_HUMAN EST388375 MAGE resequences, MAGN Homo sapiens DNA 2970 7988 4. 06 O. OE+00 AF195953. 1 NT Homo sapiens membrane-bound aminopeptidase P (XNPEP2) gene, complete cds 2973 7991 13005 6. 1 O. OE+00 5579469 NT Homo sapiens heat shock 70kD protein 1 (HSPA1A), mRNA 2973 7991 13006 6. 1 O. OE+00 5579469 NT Homo sapiens heat shock 70kD protein 1 (HSPA1A), mRNA 2975 7993 6. 5 O. OE+00 AL359403. 1 NT isoform 2 of a novel human mRNA from chromosome 22 2979 7997 13010 1.96 0. OE+00 AF017433. 1 NT Homo sapiens putative transcription factor CR53 (CR53) mRNA, partial cds Homo sapiens transcription factor IGHM enhancer 3, JM11 protein, JM4 protein, JM5 protein, T54 protein, JM10 protein, A4 differentiation-dependent protein, triple LIM domain protein 6, and synaptophysin genes, 2982 8000 1.97 0.0E+00 AF196779. 1 NT complete cds ; and L-type calcium channel a> 3002 8020 13033 3. 21 O. OE+00 X03529. 1 NT Human germline gene 16. 1 for lg lambda L-chain C region (igL-C16. 1) 3008 8025 1. 61 O. OE+00 AF199355. 1 NT Homo sapiens F-box protein FBL5 (FBL5) mRNA, complete cds 3012 8029 13040 1. 43 O. OE+00 AF064589. 1 NT Homo sapiens melanoma-associated antigen (MAGE-C1) gene, complete cds 3033 8050 13059 3.49 0. OE+00 AF265208. 1 NT Homo sapiens SWI-SNF complex protein p270 mRNA, partial cds 3034 8051 13060 5.9 0. OE+00 AF149773. 1 Nu Homo sapiens NOD1 protein (NOD1) gene, exons 1, 2, and 3 3038, 8055 130631 3. 23 O. OE+00 7662139 NT Homo sapiens KIAA0469 gene product (KIAA0469), mRNA Page 196 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO : Signal BLAST E No. Source NO: NO: Source Value 3039 8056 13064 1.38 0.0E+00 AF042075.1 NT Homo sapiens olfactory receptor-like protein (OLFR 42B) gene, OLFR 428-9110 allele, partial cds 3068 8084 13098 3. 44 O. OE+00 4826783 NT Homo sapiens potassium voltage-gated channel, Shab-related subfamily, member 1 (KCNB1) mRNA 3077 8093 13107 28. 05 O. OE+00 L20941. 1 NT Human ferritin heavy chain mRNA, complete cds 3080 8096 13110 1. 09 O. OE+00 AB011121. 1 NT Homo sapiens mRNA for KIAA0549 protein, partial cds 30801 8096 13111. 1. 09 O. OE+00 AB011121. 1 NT Homo sapiens mRNA for KIAA0549 protein, partial cds ye32f03. s1 Stratagene lung (#937210) Homo sapiens mRNA clone IMAGE : 119453 3'similar to SP : S29539 3087 8103 13118 33. 16 O. OE+00 T94870. 1 EST HUMAN S29539 BASIC PROTEIN, 23K- ; 3102 8118 13136 1. 16 0.0E+00 BF243336. 1 EST_HUMAN 601878507F1 NIH_MGC_55 Homo sapiens cDNA clone IMAGE : 4107433 5' 3104 8120 13137 1. 26 O. OE+00 AI968086. 1 EST HUMAN wu12h10. x1 NCI CGAP GC6 Homo sapiens cDNA clone IMAGE : 2516803 3' 3109 8125 13144 4.08 0.0E+00 X98922. 1 NT H. sapiens mRNA for gamma-glutamyitransferase 3109 8125 13145, 4. 08 O. OE+00 X98922. 1 NT H. sapiens mRNA for gamma-glutamyltransferase 3120 8136 13157 1. 48 O. OE+00 4758827 NT Homo sapiens neure) dn III (NRXN3) mRNA 3120 8136 13158 1. 48 O. OE+00 4758827 NT Homo sapiens neure) dn III (NRXN3) MARNA 3127 8143 13164 9. 09 O. OE+00 4504658 NT Homo sapiens interieukin 1 receptor, type (IL1R1) mRNA 3145 8161 13182 12. 76 O. OE+00 M28699. 1 NT Homo sapiens nuclear phosphoprotein B23 (NPM1) mRNA, complete cds I Homo sapiens solute carrier family 25 (mitochondrial carrier ; adenine nucleotide translocator), member 5 3149 8165 13185 10. 04 O. OE+00 4502098 NT (SLC25A5), nuclear gene encoding mitochondrial protein, mRNA 3155 8171 13193 0. 8 0.0E+00 4758055 NT Homo sapiens CREB binding protein (Rubinstein-Taybi syndrome) (CREBBP) mRNA 3155 8171 13194 0. 8 O. OE+00 4758055 NT Homo sapiens CREB binding protein (Rubinstein-Taybi syndrome) (CREBBP) mRNA 3157 8173 13195 2. 91 O. OE+00 AA774783. 1 EST_HUMAN ae87b11.s1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE : 971133 3' 3165 8181 13203 5. 5 O. OE+00 AF286598. 1 NT Homo sapiens angiostatin binding protein 1 mRNA, complete cds 3165 8181 13204 5. 5 O. OE+00 AF286598. 1 NT Homo sapiens angiostafin binding protein 1 mRNA, complete cds 3175 8191 13212 1. 57 O. OE+00 4557590 NT Homo sapiens fibrillin 1 (Marfan syndrome) (FBN1) MARNA 3180 8196 13219 0. 98 O. OE+00 4507720 NT Homo sapiens tintin (TTN) mRNA Homo sapiens HLA class III region containing tenascin X (tenascin-X) gene, partial cds ; cytochrome P450 21- hydroxylase (CYP21B), complement component C4 (C4B) G11, helicase (SKI2W), RD, complement factor B 3187 8203 13225 1.67 0.0E+00 AF019413. 1 NT (Bf), and complement component C2 (C2) genes, > 3190 8206 13228 3. 59 O. OE+00 AF055084. 1 NT Homo sapiens very large G-protein coupled receptor-I (VLGR 1) MRNA, complete cds i 3193 8209 13230 1. 14 0.0E+00 7662125 NT Homo sapiens KIAA0440 protein (KIAA0440), mRNA 3193 8209 13231 1.14 0.0E+00 7662125 NT Homo sapiens KIAA0440 protein (KIAA0440), mRNA 3201 10045 13238 2. 89 O. OE+00 4502014 NT Homo sapiens A kinase (PRKA) anchor protein 1 (AKAP1), mRNA 3201 10045 13239 2. 89 0.0E+00 4502014 NT Homo sapiens A kinase (PRKA) anchor protein 1 (AKAP1), mRNA 3218 8233 13254 2.91 0.0E+00 AF265208.1 NT Homo sapiens SWI-SNF complex protein p270 mRNA, partial cds Page 197 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Porbe Exon Most Similar ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source NO: NO: Source Value 3219 8234 13255 1.97 0.0E+00 8923624 NT Homo sapiens hypothetical protein FLJ20695 (FLJ20695), mRNA 3231 8246 13268 1. 1 O. OE+00 7657038 NT Homo sapiens death receptor 6 (DR6), mRNA tr58f08.x2 NCI CGAP Pan1 Homo sapiens mRNA clone IMAGE : 2222535 3' similar to SW : RL11 RAT 3250 8263 13284 6 O. OE+00 Api589294. 1 EST_HUMAN P25121 6oS RIBOSOMAL PROTEIN Lu 1. ; contains Alu repetitive element ; 3257 8270 13292 2. 09 O. OE+00 AF128893. 1 NT Homo sapiens telomerase reverse transcriptase (TERT) gene, exons 1-6 3257 8270 13293 2. 09 O. OE+00 AF128893. 1 NT Homo sapiens telomerase reverse transcriptase (TERT) gene, exons 1-6 3258 8271 13294 0.78 0.0E+00 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 3258 8271 13295 0. 78 O. OE+00 7657213 NT Homo sapiens hormonally upregulated neu tumor-associated kinase (HUNK), mRNA 3261 8274 13297 1. 16 O. OE+00 4502582 NT Homo sapiens caspase 8, apoptosis-related cvysteine protease (CASP8) mRNA 3261 8274 13298 1. 16 O. OE+00 4502582 NT Homo sapiens caspase 8, apoptosis-related cysteine protease (CASP8) mRNA 3264 8277 13300 11. 21 O. OE+00 AF111163. 1 NT Homo sapiens pyrin (MEFV) gene, complete cds 3266 8279 13302 1. 25 O. OE+00 AB040940. 1 NT Homo sapiens mRNA for KIAA1507 protein, partial cds wb10f0.4.x1 NCI_CGAP_GC6 Homo sapiens mRNA clone IMAGE : 2305279 3'similar to TR : Q91929 Q91929 3279 8291 13316 0. 86 O. OE+00 AI632569. 1 EST HUMAN ZINC FINGER PROTEIN. ; 3312 8323 13346 3. 08 O. OE+00 AU123664. 1 ESTHUMAN AU123664NT2RM2 Homo sapiens cDNA clone NT2RM2000735 5' 3319 8329 13349 0.95 0.0E+00 7363436 NT Homo sapiens olfactory receptor, family 10, subfamily C, member 1 (OR10C1), mRNA 3319 8329 13350 0. 95 O. OE+00 7363436 NT Homo sapiens factory receptor, family 10, subfamily C, member 1 (OR10C1), mRNA 3322 8332 13352 1. 91 O. OE+00 7706239 NT Homo sapiens neuroblastoma-amplified protein (LOC51594), mRNA 3323 8333 13353 1. 03 O. OE+00 AF211189. 1 NT Homo sapiens T-type calcium channel alpha1 subunit Alphall-a isoform (CACNA11) mRNA, complete cds 3327, 8337 0. 97 O. OE+00 AW867015. 1 EST_HUMAN MR1-SN0033-100400-001-c08 SN0033 Homo sapiens DNA 3340 8349 13366 1.39 0.0E+00 7662401 NT Homo sapiens KIAA0952 protein (KIAA0952), mRNA 3340 8349 13367 1. 39 O. OE+00 7662401 N1'Homo sapiens KIAA0952 protein (KIAA0952), mRNA 3341 8350 13368 1. 13 O. OE+00 4502398 NT Homo sapiens beaded filament structural protein 1, filension (BFSP1) mRNA Homo sapiens leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2 (LILRA2), 3343 8352 13369 2. 13 0.0E+00 5803067 NT mRNA 3352 7617 12728 1. 45 O. OE+00 AF110763. 1 NT Homo sapiens skeletal muscle LIM-protein 1 (FHL1) gene, complete cds 3357 8365 13382 2. 22 O. OE+00 7657038 NT Homo sapiens death receptor 6 (DR6), mRNA 3358 8366 13383 1. 37 O. OE+00 5453965 NT Homo sapiens protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2) mRNA 3358 8366 13384 1. 37 O. OE+00 5453965 NT Homo sapiens protein kinase, AMP-activated, alpha 2 catalytic subunit (PRKAA2) mRNA 3361 8369 13388 0.96 0.0E+00 AJ277276. 1 NT Homo sapiens MARNA for rampa-2 (rapa gene) 3361 8369 13339 0. 96 O. OE+00 AJ277276. 1 Homo sapiens mRNA for rapa-2 (rapa gene) Bacteriophage PI replication region including repa, parA, and paru genes and IncA, IncB, and inca 3362 8370 13390 4. 76 0.0E+00 K02380. 1 NT incompatibility determinants Page 198 of 209 Table 4 Single Exon Probes Expressed in HBL100 Cells Probe Exon Most Similar SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit ID NO: Signal BLAST E No. Source NO: NO: Source Value 3364 8372 13392 1.12 0.0E+00 7427522 NT Homo sapiens protein tyrosine phosphatase, receptor type, T(PTPRT), mRNA 3367 8375 13395 1. 1 O. OE+00 4557746 NT Homo sapiens met proto-oncogene (hepatocyte growth factor receptor) (MET) mRNA wp14d10.x1 NCI_CGAP_Lu19 Homo sapiens cDNA clone IMAGE : 2464819 3' similar to TR : 073634 073634 3373 8381 13400 4. 09 O. OE+00 AI935159.1 EST_HUMAN NEURAL CELL ADHESiON MOLECULE. ; p14d10.x1 NCI_CGAP_Lu19 Homo sapiens cDNA clone IMAGE : 2464819 3' similar to TR : 073634 073634 3373 8381 13401 4. 09 O. OE+00 AI935159. 1 EST HUMAIN NEURAL CELL ADHESION MOLECULE. ; 3377 8385 13406 1. 61 O. OE+00 AJ278120. 1 NT Homo sapiens mRNA for putative ankyrin-repeat containing protein (ORFA) 3385 8393 13416 5. 22 O. OE+00 6552332 NT Homo sapiens v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS), mRNA 3385 8393 13417 5. 22 O. OE+00 6552332 NT Homo sapiens v-fos FBJ murine osteosarcoma viral oncogene homolog (FOS), mRNA 3391 8399 13425 1. 11 0.0E+00 M14123. 1 NT Human endogenous retrovirus HERV-K10 3397 8405 13431 6. 63 O. OE+00 U43293. 1 NT Human MDS1A (AML1/MDS1 fusion) mRNA, partial cds 3401 8410 13435 0. 99 O. OE+00 9558718 NT Homo sapiens hypothetical protein (AF038169), mRNA 3401 8410 13436 0. 99 O. OE+00 9558718 NT Homo sapiens hypothefical protein (AF038169), MARNA 3405 8414 13441 2. 65 O. OE+00 AF045452. 1 NT fiomo sapiens cell-line KG1 transcriptional regulatory protein p54 mRNA, complete cds 3405 8414 13442 2. 65 O. OE+00 AF045452. 1 NT Homo sapiens cell-line KG1 transcriptional regulatory protein p54 mRNA, complete cds 3413 8422 13451 1. 04 O. OE+00 AF231922. 1 NT Homo sapiens chromosome 21 unknown MARNA 3423 8431 13457 1. 44 O. OE+00 4508028 NT Homo sapiens zinc finger protein 45 (a Kruppel-associated box (KRAB) domain polypeptide) (ZNF45) mRNA 3426 8434 13459 2. 04 O. OE+00 8E304791. 1 EST_HUMAN 601143853f1 NIH_MGC_15 Homo sapiens mRNA clone IMAGE : 3051373 5' 3426 8434 13460 2. 04 O. OE+00 BE304791. 1 EST_HUMAN 601143853F1 NIH_MGC_16 Homo sapiens cDNA clone IMAGE : 3051373 5' 3429 8437 13463 1. 11 O. OE+00 4826795 NT Homo sapiens potassium voltage-gated channel, Isk-related family. member 2 (KCNE2) mRNA 3431 8439 1346 1. 05 O. OE+00 014867 SWISSPROT TRANSCRIPTION REGULATOR PROTEIN BACH1 (BTB AND CNC HOMOLOG 1) (HA2303) tge35g12.x1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE : 2088742 3' similar to TR : 000498 3436 8444 13470 0. 83 O. OE+00 A1 384007. 1 EST HUMAIN 000498 MYASTHENIA GRAVIS AUTOANTIGEN GRAVIN ; 3439 8447 13473 1. 05 O. OE+00 M10976. 1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 3454 8462 13489 1. 53 O. OE+00 AB032979. 1 NT Homo sapiens mRNA for KIAA1153 protein, partial cds 3454 8462 13490 1. 53 O. OE+00 AB032979. 1 NT Homo sapiens mRNA for KIAA1153 protein, partial cds 3463 8471 13496 0. 86 O. OE+00 AV701869. 1 EST_HUMAN AV701869 ADB Homo sapiens cDNA clone ADBDAH06 5' 3465 8473 13497 1. 08 O. OE+00 4506884 NT Homo sapiens semenogelin 11 (SEMG2) mRNA 3467 8475 2. 85 O. OE+00 AF078868. 1 NT Homo sapiens homologous yeast-44. 2 protein mRNA, complete cds 3475 8483 13503 1. 11 O. OE+00 AL133204. 1 NT Novel human gene mapping to chomosome X 3477 8485 13504 0. 77 O. OE+00 AB040909. 1 VINT Homo sapiens mRNA for KIAA1476 protein, partial cds 3485 8493 13510 1.54 0.0E+00 8923087 NT Homo sapiens hypothetical protein FLJ20080 (FLJ20080), mRNA 3493 8501 13515 0.94 0. OE+00 6997248 NT Homo sapiens sal (Drosophila)-like 1 (SALL1), mRNA Page 199 of 209 Table 4 Single Exon Probes Expressed in HBL100 Probe Exon Most Similar ORS SET Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source NO: NO: Source Value 3493 8501 13516 0.94 0.0E+00 6997248 NT Homo sapiens sal (Drosophila)-like 1 (SALL1), mRNA ox77c11.x1 Soares NhHMPu S1 Homo sapiens cDNA clone IMAGE : 1662356 3'similar to WP : T19B4. 4 3494 8502 1. 7 O. OE+00 A1081907. 1 EST HUMAIN CE13742 ; 3496 8504 13519 1. 01 O. OE+00 6325463 NT Homo sapiens butyrophilin, subfamily 3, member A3 (BTN3A3), mRNA 3500 8508 4. 62 O. OE+00 AW852217. 1 EST_HUMAN QV0-CT0225-230300-169-e01 CT0225 Homo sapiens cDNA 3504 8512 135271 1. 42 O. OE+00 4504294 NT Homo sapiens H3 histone family, member K (H3FK), mRNA 3508 8516 5. 83 O. OE+00 AF118846. 1 NT Homo sapiens gamma-glutamylcysteine synthetase (GLCLC) gene, partial cds 3509 8517 13528 7. 79 O. OE+00 BF676393. 1 EST_HUMAN 602084583F1 NIH_MGC_83 Homo sapiens mRNAclone IMAGE : 4248596 5' oq94h06.s1 NCI_CGAP_Kid6 Homo sapiens cDNA clone IMAGE : 1594043 3' similar to contains MER29. b2 3513 8521 1. 01 O. OE+00 AA988715. 1 EST HUMAIN MER29 repetitive element ; 3522 8529 13539 1. 4 O. OE+00 AW937977. 1 EST_HUMAN QV0-Dt0047-170200-123-g01 DT0047 Homo sapiens cDNA 3535 8541 13547, 0. 96 O. OE+00 BF672054. 1 EST_HUMAN 602152486F1 NIH_MGC_81 Homo sapiens cDNA clone IMAGE : 4293645 5' 3535 8541 13548 0. 96 O. OE+00 BF672054. 1 EST_HUMAN 602152486F1 NIH_MGC_81 Homo sapiens cDNA clone IMAGE : 4293645 5' 3536 8542 1. 13 O. OE+00 4826967 NT Homo sapiens retinoblastoma-binding protein 2 (RBBP2) mRNA 3538 8544 13550 0. 75 O. OE+00 AW664693. 1 EST_HUMAN hi84g01.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2979024 3' 3538 8544 13551 0. 75 O. OE+00 AW664693. 1 EST_HUMAN hi84g01.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2979024 3' 3541 8547 13555 0.72 0.0E+00 7662319 NT Homo sapiens KIAA0806 gene product (KIAA0806), mRNA 3546 8553 13560 2.09 0.0E+00 4557752 NT Homo sapiens midline 1 (Optz/BBB syndrome) (MID1) mRNA 3546 8553 13561 2. 09 O. OE+00 4557752 NT Homo sapiens midline 1 (Optiz/BBB syndrome) (MID1) mRNA 3562 8569 13574 2. 29 O. OE+00 D87327. 1 NT Homo sapiens mRNA for G protein-coupled inward rectifier potassium channel, complete cds 3566 8573 14. 47 O. OE+00 7669491 NT Homo sapiens glyceraidehyde-3-phosphate dehydrogenase (GAPD), mRNA 3583 8590 13594 3. 35 O. OE+00 AB026542. 1 NT Homo sapiens WAVE2 mRNA for WASP-family protein, complete cds 3584 8591 13595 1. 92 O. OE+00 AB007866. 2 NT Homo sapiens mRNA for KIAA0406 protein, partial cds 3586 8593 13596 4.2 0. OE+00 AF124250. 1 NT Homo sapiens SH2-containing protein Nsp2 mRNA, complete cds 3586 8593 13597 4. 2 O. OE+00 AF124250. 1 NT Homo sapiens SH2-containing protein Nsp2 mRNA, complete cds 3592 8599 13605 1. 04 O. OE+00 AA852743. 1 EST_HUMAN NHTBCae15g09f1 Normal Human Trabecular Bone Cells Homo sapiens mRNA clone NHTBCae15g09 3592 8599 13606 1. 04 O. OE+00 AA852743. 1 EST_HUMAN NHTBCae15g09f1 Normal Human Trabecular Bone Celles homo sapiens cDNA clone NHTBCae15g09 3595 8602 13608 1. 83 O. OE+00 AL163204. 2 NT Homo sapiens chromosome 21 segment HS21 C004 3595 8602 13609 1.83 0.0E+00 AL163204. 2 NT Homo sapiens chromosome 21 segment HS21 C004 3596 8603 13610 0. 93 O. OE+00 AB002331. 1 NT Human mRNA for KIAA0333 gene, partial cds 3596 8603 13611 0. 93 O. OE+00 Ab002331. 1 NT Human mRNA for KIAA0333 gene, partial cds 3599 8606 13614 1. 43 O. OE+OOAW851714. 1 EST_HUMAN MR2-CT0222-281099-005-e05 CT0222 Homo sapiens cDNA Page 200 of 209 Table 4 Single Exon Probes Expressed in HBL100 Probe Exon Most Similar ORFSEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLST E No. Source Value 3601 8608 13616 1. 91 O. OE+00 5729928 NT Homo sapiens matrix metalloproteinase 24 (membrane-inserted) (MMP24), mRNA 3603 8610 13618 1.09 0. OE+00 AB018339. 1 NT Homo sapiens mRNA for KIAA0796 protein, partial cds 3605 8612 13620 1 0. OE+00 014867 SWISSPROT TRANSCRIPTION REGULATOR PROTEIN BACH1 (BTB AND CNC HOMOLOG 1) (HA2303) 3607 8614 13622 0.83 0. OE+00 AB020717.1 NT Homo sapiens mRNA for KIAA0910 protein, partial cds 3607 8614 13623 0.83 0. OE+00 AB020717. 1 NT Homo sapiens mRNA for KIAA0910 protein, partial cds 3618 8625 13632 1.01 0. OE+00 5729733 NT Homo sapiens activator of S phase kinase (ASK) mRNA 3618 8625 13633 1. 01 O. OE+00 5729733 NT Homo sapiens activator of S phase kinase (ASK) MARNA 3623 8630 13635 4.46 0. OE+00 AW298134. 1 EST_HUMAN UI-H-BW0-ajs-e-12-0-UI.s1 NCI_CGAP_Sub6 Homo sapiens cDNA clone IMAGE : 2733022 3' 3623 8630 13636 4.46 0. OE+00 AW298134. 1 EST_HUMAN UI-H-BW0-ajs-e-12-0-UI.s1 NCI_CGAP_Sub6 Homo sapiens cDNA clone IMAGE : 2733022 3' 3645 8651 13657 1.21 0. OE+00 AB004630. 1 NT Human gene for Type XIX collagen a1 chain, exon 6 aa06g01.r1 Soares_NhHMPu_S1 Homo sapiens cDNA clone IMAGE : 812496 5'similar to 3646 8652 13658 0.9 0. OE+00 AA463659. 1 EST HUMAIN SW : KRB4_SHEEP P02445 KERATIN, HIGH-SULFUR MATRIX PROTEIN, IIIB4. [1] ; 3650 8656 13662 1.09 0. OE+00 AB020710. 1 NT Homo sapiens mRNA for KIAA0903 protein, partial cds 36531 8659 13664 4. 19 O. OE+00 7657468 NT Homo sapiens similar to rat integral membrane glycoprotain POM121 (POM121 L1), mRNA 36741 8679 13682 7. 89 O. OE+00 7662183 NT Homo sapiens KIAA0569 gene product (KIAA0569), mRNA 3677 8682 13685 75.43 0. OE+00 4506718 NT Homo sapiens ribosomal protein S2 (RPS2) mRNA 3683 8687 13689 1.39 0.0E+00 7657065 NT Homo sapiens v-ets avian erythroblastosis virus E26 oncogene related (ERG), mRNA 3683 8687 13690 1. 39 O. OE+00 7657065 NT Homo sapiens v-ets avian erythroblastosis virus E26 oncogene related (ERG), mRNA 3729 8733 0.98 0. OE+00 AF195658. 1 NT Homo sapiens DNA mismatch repair protein (MLH3) gene, complete cods 3730 8734 13731 2. 82 O. OE+00 AF179733. 1 NT Pan troglodytes olfactory receptor (PTR208) gene, partial cds 3733 8737 13735 2.36 0.0E+00 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM121 (POM121L1), mRNA 3733 8737 13736 2.36 0.0E+00 7657468 NT Homo sapiens similar to rat integral membrane glycoprotein POM121 (POM121L1), mRNA 3734 8738 13737 1.15 0. OE+00 AF020091. 1 NT Homo sapiens smooth muscle myosin heavy chain SM1 mRNA, alternatively spliced, partial cds 3738 8742 13742 1.16 0. OE+00 4759011 NT Homo sapiens RAB9, member RAS oncogene family (RAB9) mRNA 3741 8745 13745 1.16 0. OE+00 AF127851. 1 NT gorilla gorilla olfactory receptor (GG071) gene, partial cods 3741 8745 13746 1.16 0.0E+00 AF127851. 1 NT Gorilla gorilla olfactory receptor (GG071) gene, partial cds 3742 8746 13747 1.18 0.0E+00 AI377699.1 EST_HUMAN te62f10.x1 Soares_NFL_T_GBC_S1 Homo sapiens cDNA clone IMAGE : 2091307 3' 3743 8747 1.17 0. OE+00 AF152496. 1 NT Homo sapiens protocadherin beta 3 (PCDH-beta3) mRNA, complete cds 3744 8748 13748 1.26 0. OE+00 4758199 NT Homo sapiens desmoplakin (DPI, DPII) (DSP) mRNA 3747 8751 13750 36.36 0. OE+00 S78685. 1 NT Homo sapiens ATP-sensitive inwardly rectifying K-channel subunit (KCNJ6/BIR1) gene, complete cds 3749 8753 13752 2.74 0.0E+00 7710148 NT Homo sapiens method CpG binding protein 2 (MECP2), mRNA 3750 8754 13753 1.32 0. OE+00 7662183 NT Homo sapiens KIAA0569 gene product (KIAA0569), mRNA 3752 8756 13754 1.1 0.0E+00 AF069601.2 NT Homo sapiens myosin light chain kinase isoform 2 (MLCK) mRNA, complete cds Page 201 of 209 Table 4 Single Exon Probes Expressed in HBL100 Probe Exon Most Similar Top Hit ORF SEQ Expression Expression (Top) Top Hit Acession pabase SEQ ID SEQ ID p NO : Signal BLAST E No. Top Hit Descriptor Source NO : N : Value Vaiue 3752 8756 13755 1. 1 O. OE+00 AF069601. 2 NT Homo sapiens myosin light chain kinase isofam 2 (MLCK) mRNA, complete cds 3753 8757 13756 1. 03 O. OE+00 4504534 NT Homo sapiens 5-hydroxytryptamine (serotonin) recepta 1 D (HTR1 D) mRNA 3759 8762 13762 0. 81 O. OE+00 6912735 NT Homo sapiens transient receptor potential channel 5 (TRPC5), mRNA 3764 8767 13770 7. 3 O. OE+00 4503178 NT Homo sapiens chromosome X open reading frame 5 (CXORF5) mRNA 3764 8767 13771 7. 3 O. OE+00 4503178 NT Homo sapiens chromosome X open reading frame 5 (CXORF5) mRNA 3766 8769 13774 4. 16 O. OE+00 U09412. 1 NT Human zinc finger protein ZNF134 mRNA, complete cds 3767 8770 13775 1. 26 O. OE+00 AF114488. 1 NT t-iomo sapiens intersectin short isoform (ITSN) mRNA, complete cds 3770 8773 13777 1. 15 O. OE+00 4826783 NT Homo sapiens potassium voltage-gated channel, Shab-related subfamily, member 1 (KCNB1) mRNA 3773 8776 13780 2. 04 O. OE+00 4759171 NT Homo sapiens SC35-interacting protein 1 (SRRP129), mRNA wk01f01. x1 NCI_CGAP Lym12 Homo sapiens cDNA clone IMAGE : 2411065 3'similar to TR : 043340 3784 8787 13791 2. 48 O. OE+00 AI864727. 1 EST HUMAN 043340 R28830 2. ; contains element PTR7 repetitiva element ; g7g7 g7g0 13795 20. 27 O. OE+00 4506742 NT Homo sapiens ribosomal protein S8 (RPS8), mRNA g7gg g7g2 13797 1. 47 O. OE+00 AL040338. 1 EST HUMAN DKFZp434N0413 r1 434 (synonym : htes3) Homo sapiens cDNA clona DKFZp434N0413 5' 3794 8797 13803 0. 97 O. OE+00 6005887 NT Homo sapiens AP1 gamma subunit binding protein 1 (AP1 GBP1), mRNA 3794 8797 13804 0. 97 O. OE+00 6005887 NT Homo sapiens AP1 gamma subunit binding protein 1 (AP1 GBP1), mRNA 3795 8798 13805 1. 95 O. OE+00 4504138 NT Homo sapiens 9lutamate recepta, metabotropic 3 (GRM3) mRNA 3796 8799 1. 87 O. OE+00 4505078 NT Homo sapiens melanoma an6gen, family B, 1 (MAGEB1) mRNA 3800 8803 13808 0. 87 O. OE+00 AF149412. 1 NT Homo sapiens HBP17 heparin-binding and FGF-binding protein gene, complete cds 3808 8811 13817 1. 69 O. OE+00 4506758 NT Homo sapiens ryanodine reptw 3 (RYR3) mRNA 3812 8815 13821 2. 31 O. OE+00 4585642 NT Homo sapiens anc finges protein (KIAA0412) mRNA 3824 8826 13833 1. 91 O. OE+00 AF129533. 1 NT Homo sapiens F-box protein Fbl3b (FBL3B) mRNA, partial cds. 3829 8831 13838 3. 61 O. OE+00 BE378602. 1 EST HUMAN 601236966F1 NIH MGC 44 Homo sapiens cDNA clone IMAGE : 3608800 5' 3836 8838 13844 2. 13 O. OE+00 AW 580740. 1 EST HUMAN PM3-LT0031-100100-003-h09 LT0031 Homo sapiens cDNA 3837 8839 13845 1. 01 O. OE+00 BE264998. 1 EST HUMAN 601193827F1 NIH MGC 7 Homo sapiens cDNA clone IMAGE : 3537774 5' 3837 8839 13846 1. 01 O. OE+00 BE264998. 1 EST HUMAN 601193827F1 NIH MGC 7 Homo sapiens cDNA clone IMAGE : 3537774 5' 3868 8869 13872 4. 2 O. OE+00 AF116195. 1 NT Homo sapiens cancer-tesfis anfigen CT10 (CT10) gene, complete cds 3868 8869 13873 4. 2 O. OE+00 AF116195. 1 NT Homo sapiens cancer-tesds antigen CT10 (CT10) gene, complete cds O. OE+00 M23910. 1 NT Human MHC class Ii lymphocyte an6gen DPw4-beta-2 pseudogene, exon 2 3881 8882 5. 99 O. OE+00 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21 C103 3887 8888 13887 0. 93 O. OE+00 AL118494. 1 NT Novel human gene mapping to chomosome 20 3891 8891 13889 2. 94 O. OE+00 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 gggg gggg 13897 2. 2 O. OE+00 AL163268. 2 NT Homo sapiens chromosoma 21 segment HS21 C068 3911 8911 113. 41 O. OE+00 4503470 NT Homo sapiens eufcaryobc transla6on elongation facta 1 alphe 1 (EEF1A1) mRNA Page 202 of 209 Table 4 Single Exon Probes Expressed in HBL100 D h c Most Simitar-... Probe Exon Most Similar Top Hit SEQ ID SEQ ID RF SEQ Expression (Top) Hit Top Hit Acession pase Top Hit Descriptor NO : NO : ID NO : Signal BL. AST E N Source, Value tt55g08. x1 NCI_CGAP_GC6 Homo sapiens cDNA clone IMAGE : 2244734 3'similar to TR : 060309 060309 3914 8914 1. 34 O. OE+00 AI657076. 1 EST HUMAN KIAA0563 PROTEIN. ; 3916 8996 13910 2. 58 O. OE+00 U09366. 1 NT Human zinc finger protein ZNF133 3933 8932 13925 12. 81 O. OE+00 AB015610. 1 NT Chlorocebus aethiops mRNA for ribosorttal protein S4X, complete cds 3941 8939 3. 58 O. OE+00 AJ238617. 1 NT Homo sapiens mRNA fa UGA suppressor tRNA-associated an6genic protein (tRNA48 gene) 3951 8949 13938 1. 39 O. OE+00 AB002314. 2 NT Homo sapiens mRNA fa KIAA0316 protein, partial cds 3952 8950 13939 1. 76 O. OE+00 AL163203. 2 NT Homo sapiens chromosome 21 segment HS21 C003 3953 8951 13940 2. 58 O. OE+00 AJ277276. 1 NT Homo sapiens mRNA for rapa-2 (rapa gene) 3953 8951 13941 2. 58 O. OE+00 AJ277276. 1 NT Homo sapiens mRNA fa rapa-2 (rapa gene) 3961 8959 93948 14. 88 O. OE+00 5032026 NT Homo sapiens retinoblastoma-binding protein 4 (RBBP4) mRNA 3961 8959 13949 14. 88 O. OE+00 5032026 NT Homo sapiens retinoblastoma-binding protein 4 (RBBP4) mRNA Homo sapiens phosphoribosylglycinamide formyltransferase, phosphaibosylglycinamide synthetase, 3969 8968 13957 1. 91 O. OE+00 4503914 NT phosphoribosylaminamidazole synthetase (GART) mRNA 3973 8971 13959 4. 78 O. OE+00 4$85306 NT Homo sapiens G protein-coupled recepta 21 (GPR21), mRNA 3974 8972 13960 1. 41 O. OE+00 AB006625. 1 NT Homo sapiens mRNA for KIAA0287 gene, partial cds 3977 8975 13961 0. 8 O. OE+00 4758807 NT Homo sapiens ras GTPase activating protein-like (NGAP) mRNA 3978 8976 13962 7. 12 O. OE+00 11419297 NT Hano sapiens IMP (inosine monophosphate) dehydrogenase 1 (IMPDH1), mRNA 3979 8977 13963 3. 68 O. OE+00 AL096857. 1 NT fVovef human mRNA from chromosome 1, which has similari6es to BAT2 genes ze55e09. r1 Soares refina N2b4HR Homo sapiens cDNA clone IMAGE : 362920 5'similar to contains Alu 3980 8978 0. 98 O. OE+00 AA018975. 1 EST HUMAN repetitive element ; 3986 8984 13970 3. 76 O. OE+00 AF165527. 1 NT Homo sapiens DGCRS (DGCR8) mRNA, complete cds 3991 10050 13974 1. 98 O. OE+00 AF157476. 1 NT Homo sapiens DNA polymerase zata catalytic subunit (REV3) mRNA, complete cds 3995 6121 11150 1. 11 O. OE+00 4826947 NT Homo sapiens protein kinase, X tinke (PRKX) mRNA 3995 6121 11151 1. 11 O. OE+00 4826947 NT Homo sapiens protein kinase, X linked (PRKX) mRNA 4000 8996 13984 1. 04 O. OE+00 4503854 NT Hano sapiens GA-binding protein transcrip6on factor, alpha subunit (60kD) (GABPA), mRNA 4000 8996 13985 1. 04 O. OE+00 4503854 NT Homo sapiens GA-binding protein transcription factor, alpha subunit (60kD) (GABPA), mRNA 4003 8999 13987 1. 39 O. OE+00 8922391 NT Homo sapiens hypothetical protein FLJ10379 (FLJ10379), mRNA 4003 8999 13988 1. 39 O. OE+00 8922391 NT Homo sapiens hypotheticai protein FLJ10379 (FLJ10379), mRNA 4006 9002 13989 1. 03 O. OE+00 AB020702. 1 NT Homo sapiens mRNA for KIAA0895 protein, partial cds 4012 9008 13997 6. 13 O. OE+00 AI982597. 1 EST HUMAN wu04d04. c'1 NCI_CGAP GC6 Homo sapiens cDNA clone IMAGE : 2515975 3' 4012 9008 13998 6. 13 O. OE+00 Ai982597. 1 EST HUMAN wu04d04. x1 NCI_CGAP GC6 Homo sapiens cDNA clone IMAGE : 2515975 3' 4014 9010 14000 2. 24 O. OE+00 BE184856. 1 EST_NUMAN MR1-NT0707-900500-001-e02 HT0707 Homo sapiens cDNA 4014 9010 14001 2. 24 O. OE+00 BE184856. 1 EST HUMAN MR1-HT0707-100500-001-a02 HT0707 Homo sapiens cDNA 4018 9014 10. 6 O. OE+00 BE274217. 1 EST HUMAN 601120778F1 NIH MGC 20 Homo sapiens cDNA clone IMAGE : 2967690 Page 203 of 209 Table 4 Sinale Exon Probes Exaressed in HBL100 Most Similar Probe Exon ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Source Value Homo sapiens transglutaminase 3 (E polypeptide, protein-glutamine-gamma-glutamyitransferase) (TGM3) 4024 9020 14007 1. 02 O. 0E+00 4507476 NT mRNA 4025 9021 14008 2. 26 O. OE+00 5729725 NT Homo sapiens nuclear receptor coactivator 3 (NCOA3), mRNA ba51f04.x1 NIH_MGC_10 Homo sapiens cDNA clone IMAGE : 2900095 3'similar to SW : THI2_BOVIN 4032 9028 6. 78 O. 0E+00 AW675599. 1 EST HUMAN Q95108 MITOCHONDRIAL THIOREDOXIN PRECURSOR ; 4037 9033 14021 0. 95 O. OE+00 AW408788. 1 EST_HUMAN UI-HF-BM0-adx-c-02-0-UI.r1 NIH_MGC_38 Homo sapiens cDNA clone IMAGE : 3063147 5' 4039 9035 14024 1. 51 O. 0E+00 8922466 NT Homo sapiens hypothetical protein FLJ10498 (FLJ10498), mRNA 4039 9035 14025 1. 51 O. OE+00 8922466 NT Homo sapiens hypothetical protein FLJ10498 (FLJ10498), MARNA Homo sapiens polycystic kidney disease (polycystin) and REJ (sperm receptor for egg jelly, sea urchin 4048 9044 2. 18 O. 0E+00 5174632 NT homolog)-like (PKDREJ) mRNA zu68h07. s1 Soares testis NHT Homo sapiens cDNA clone IMAGE : 743197 3'similar to contains Alu 4068 9062 14049 9. 69 O. OE+00 AA401438. 1 EST HUMAN repetitive element ; contains element MER35 repetitive element ; zu68h07. s1 Soares_testis_NHT Homo sapiens cDNA clone IMAGE : 743197 3'similar to contains Alu 4068 9062 14050 9. 69 O. OE+00 AA401438. 1 EST_HUMAN repetitive element ; contains element MER35 repetitive element ; 4072 9066 14056 1. 32 O. OE+00 AF157476. 1 NT Homo sapiens DNA polymerase zeta catalytic subunit (REV3) mRNA, complete cds 4085 9079 14068 0. 94 O. OE+00 4507720 NT Homo sapiens brin (TTN) mRNA 4085 9079 14069 0. 94 O. OE+00 4507720 NT Homo sapiens Butin (TTN) mRNA 4087 9081 14072 1. 45 O. OE+00 7662125 NT Homo sapiens KIAA0440 protein (KIAA0440), mRNA 4101 9095 14079 1. 28 0.0E+00 4758199 NT Homo sapiens desmoplakin (DPI, DPII) (DSP) mRNA 4101 9095 14080 1. 28 O. OE+00 4758199 NT Homo sapiens desmoplakin (DPI, DPII) (DSP) mRNA 4109 9103 0. 71 O. 0E+00 AL163303. 2 NT Homo sapiens chromosome 21 segment HS21C103 4135 9130 14113 1. 99 O. OE+00 AJ003145. 1 NT Homo sapiens mRNA for olfactory receptor protein, pseudogene 4151 9146 14130 2. 21 O. 0E+00 J02610. 1 NT Human apolipoprotein B-100 mRNA, complete cds 4164 9159 14146 0. 86 O. OE+00 AW936689. 1 EST_HUMAN PM2-DT0023-080300-004-a08 DT0023 Homo sapiens cDNA 4171 9166 14153 4. 94 O. OE+00 AF174590. 1 NT Homo sapiens F-box protein Fbl4 (FBL4) mRNA, partial cds qd23f06.x1 Soares_placenta_8to9weeks_2NbHP8to9W Homo sapiens cDNA clone IMAGE : 1724579 3' 4177 9171 3 O. OE+00 AJ189844. 1 EST HUMAN similar to contains MER20. b2 MER20 repetitive element ; 4180 9173 5. 07 O. OE+00 U14520. 1 NT Human CBFA3 (Cbfa3) gene, partial cds Homo sapiens myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog) ; translocated to, 4 4183 9176 14160 0. 82 O. OE+00 5174574 NT (MLLT4) mRNA 4198 9191 14173 1. 19 O. OE+00 6563384 NT Homo sapiens protein kinase C, nu (PRKCN), mRNA 4198 9191 14174 1.19 O. 0E+00 6563384 NT Homo sapiens protein kinase C, nu (PRKCN), mRNA 4205 9198 14180 1.28 O. OE+QO U10991. 1 NT Human G2 protein mRNA, partial cds 4205 9198 14181 1. 28 O. OE+00 U10991. 1 VINT Human G2 protein mRNA, partial cds Page 204 of 209 Table 4 Single Exon Probes Expressed in HBL100 Porbe Exon Most Similar SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit @Q ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source Value 4215 9208 14187 10.08 0.0E+00 6912281 NT Homo sapiens COMPLEMENT COMPONENT C1q RECEPTOR (C1QR), mRNA 4235 9229 1.05 0. OE+00 AF153047. 2 NT Homo sapiens gap junction protein connexin-36 (CX36) gene, complete cds Homo sapiens plasma membrane calcium ATPase isoform 1 (ATP2B1) gene, alternative splice products, 4245 9239 14222 5. 12 O. OE+00 L14561. 1partial cds 4249 9243 14227 4.71 0. OE+00 Z80780. 1 N H.sapiens H2B/h gene 4249 9243 14228 4.71 0. OE+00 Z80780. 1 NT H. sapiens H2B/h gene xg68e10.x1 NCI_CGAP_Ut4 Homo sapiens mRNA clone IMAGE : 2633514 3'similar to TR : P97365 P97365 4250 9244 14229 1. 5 O. OE+00 AW166933. 1 EST HUMAN ZINC FINGER PROTEIN 64 ; 4256 9250 14235 2.06 0. OE+00 X60483. 1 NT H. sapiens H4/d gene for H4 histone 4256 9250 14236 2. 06 O. OE+00 X60483. 1 NT H. sapiens H4/d gene for H4 histone 4261 9254 14242 11. 18 O. OE+00 7662091 NT Homo sapiens KIAA0390 gene product (KIAA0390), mRNA 42619254 14243 11.18 0. OE+00 7662091 NT Homo sapiens KIAA0390 gene product (KIAA0390), mRNA 4274 9267 14258 11. 95 O. OE+00 4885126 NT Homo sapiens caudal type homeo box transcription factor 4 (CDX4), mRNA 4275 9268 14259 1. 08 O. OE+00 AJ271736. 1 NT Homo sapiens Xq pseudoautosomal region ; segment 2/2 4276 9269 0.99 0. OE+00 AL163207. 2 NT Homo sapiens chromosome 21 segment HS21C007 4309, 9301 14286 1. 08 0.0E+00 7019456 NT Homo sapiens myosin regulatory light chain interacting protein (MIR), mRNA 4320 9312 6.33 0.0E+00 AF195953.1 NT Homo sapiens membrane-bound aminopeptidase P (XNPEP2) gene, complete cds 4325 9317 14299 2. 96 O. OE+00 AJ249765. 1 NT Homo sapiens ACTN2 gene for alpha-Acfinin 2, exon 10 4325 9317 14300 2.96 0. OE+00 AJ249765. 1 NT Homo sapiens ACTN2 gene for alpha-Actinin 2, exon 10 4330 9321 14306 0. 81 O. OE+00 W 26179. 1 EST_HUMAN 24g7 Human retina cDNA randomly primed sublibrary Homo sapiens DNA 4330 9321 14307 0. 81 O. OE+00 W26179. 1 EST_HUMAN 24g7 Human retina cDNA randomly primed sublibrary Homo sapiens cDNA 4344 9335 2. 03 O. OE+00 AF200629. 1 NT Homo sapiens HPS1 gene, intron 5 4362 9353 0. 76 O. OE+00 M14123. 1 NT Human endogenous retrovirus HERV-K10 xc68e08. x1 NCI CGAP Eso2 Homo sapiens cDNA clone IMAGE : 2589446 3'similar to SW : AHNE HUMAIN 4372 9364 14344 2.57 0. OE+00 AW084964. 1 EST-HUMAN Q09666 NEUROBLAST DIFFERENTIATION ASSOCIATED PROTEIN AHNAK ; 4374 10052 1. 15 0.0E+00 8051619 NT Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a, mRNA Homo sapiens vascular endothelial cell growth factor 165 receptor/neuropilin (VEGF165) mRNA, complete 4375 9366 14346 0. 91 O. OE+00 AF016050. 1 NT cds 4378 9369 7. 59 O. OE+00 AL163207. 2 NT Homo sapiens chromosome 21 segment HS21 C007 4380 9371 14350 1. 24 O. OE+00 AW381570. 1 EST_HUMAN PM1-HT0305-101199-002-d03 HT0305 Homo sapiens cDNA 4386 9377 14357 1. 16 0.0E+00 AJ278120. 1 NT Homo sapiens mRNA for putative ankyrin-repeat containing protein (ORFA) 4386 9377 14358 1.16 0. OE+00 AJ278120. 1 NT Homo sapiens mRNA for putative ankyrin-repeat containing protein (ORFA) 4388 9379 14360 1.24 0.0E+00 4758467 NT Homo sapiens G protein-coupled receptor 50 (GPR50) mRNA 4389 9380 14361 3.1 O. 0E+00 AF108830.1 NT Homo sapiens serine-threonine protein kinase (MNBH) mRNA, complete cds Page 205 of 209 Table 4 Sinale Exon Probes Expressed in HBL100 Probe Exon Most Similar ORFSEQ Expression (Top) Hit Top Hit Acession Top Hit SEQ ID SEQ ID ID NO: Signal BLAST E No. Database Top Hit Descriptor NO: NO: source Value 4398 9389 14372 1.05 0.0E+00 S78684.1 NT Homo sapiens ATP-sensitive inwardly rectifying K-channel subunit (KCNJ6/BIR1) gene, exon 4399 9390 14373 1. 06 O. OE+00 AF111163. 1 NT Homo sapiens pyrin (MEFV) gene, complete cds 4399 9390 14374 1. 06 O. OE+00 AF111163. 1 NT Homo sapiens pyrin (MEFV) gene, complete cds 4409 10053 14384 3. 16 O. OE+00 6005973 NT Homo sapiens zinc finger protein 195 (ZNF195), mRNA 4413 9403 14388 5. 97 O. OE+00 AF208161. 1 NT Homo sapiens syncytin precursor, mRNA, complete cds 4418 99408 14395 1.83 0. OE+00 AF152337. 1 NT Homo sapiens protocadherin gamma C3 (PCDH-gamma-C3) mRNA, complete cds 4421 9411 14399 1. 07 O. OE+00 5454175 NT Homo sapiens zinc finger protein 211 (ZNF211), mRNA 4431 9421 14407 50.75 0.0E+00 4503470 NT Homo sapiens eukaryotic translation elongation factor 1 alpha 1 (EEF1A1) mRNA 4438 9428 14412 1. 01 O. OE+00 4505016 NT Homo sapiens low density lipoprotein receptor-related protein 6 (LRP6) mRNA, and translated products 4442 9432 14415 1. 5 O. OE+00 4503098 NT Homo sapiens chondroiun sulfate proteoglycan 4 (melanoma-associated) (CSPG4), mRNA 4446 9436 14420 1.11 0.0E+00 4602566 NT Homo sapiens calcium/calmodulin-dependent protein kinase IV (CAMK4) MARNA 4449 9439 1. 16 O. OE+00 BE871908. 1 EST HUMAN 601447932FI NIH-MGC-65 Homo sapiens cDNA clone IMAGE : 3852127 5' 4452 9442 2. 72 O. OE+00 L35485. 1 NT Homo sapiens iduronate sulphate sulphatase (IDS) gene, complete cds 4454 9444 14423 11.71 0. OE+00 7662091 NT Homo sapiens KIAA0390 gene product (KIAA0390), mRNA 4454 9444 14424 11. 71 O. OE+00 7662091 NT Homo sapiens KIAA0390 gene product (KIAA0390), mRNA 4471 9461 14440 2. 89 O. OE+00 AF143314. 1 NT Homo sapiens PTEN (PTEN) gene, exons 3 through 5 Homo sapiens mRNA for G7c protein (G7c gene located in the class III region of the major histocompatibility 4473 9463 1442 10.04 0. OE+00 AJ245418. 1 NT complex) Homo sapiens mRNA for G7c protein (G7c gene located in the class III region of the major histocompatibility 4473 9463 14443 10. 04 0. OE+00 AJ245418. 1 NT complex) 4475 9465 14444 0. 8 O. OE+00 AB018338. 1 NT Homo sapiens mRNA for KIAA0795 protein, partirai cds 4490 9480 1. 45 O. OE+00 AA174072. 1 EST_HUMAN zp18g08.s1 Stratagene fetal retina 937202 Homo sapiens cDNA clone IMAGE : 609854 3' 4492 9482 1. 39 O. OE+00 7657410 NT Homo sapiens odz (odd Ozlten-m, Drosophila) homolog 1 (ODZ1), mRNA 4494 9484 2. 8 O. OE+00 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 4495 9485 14463 1. 18 O. OE+00 AF184110. 1 NT Homo sapiens cyclophilin-related protein (NKTR) gene, compete cds 496 9486 14464 5. 1 O. OE+00 AL163300. 2 NT Homo sapiens chromosome 21 segment HS21C100 4497 9487 1. 83 0.0E+00 AB037521. 1 NT Homo sapiens gene for natriuretic protein, partial cds 4499 9489 14465 0. 9 O. OE+00 AF195658. 1 NT Homo sapiens DNA mismatch repair protein (MLH3) gene, complete cds 4502 9492 14469 1. 33 O. OE+00 AB007866. 2 NT Homo sapiens mRNA for KIAA0406 protein, partial cds 4507 9497 14475 11. 33 O. OE+00 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA 4507 9497 14476 11. 33 0.0E+00 4557887 NT Homo sapiens keratin 18 (KRT18) mRNA 4508 9498 14477 2. 06 0. OE+00 AF167441. 1 NT Mus musculus E-cadherin binding protein E7 mRNA, complete cds 4518 9508 14489 0. 94 0.0E+00 L78810. 1 NT Homo sapiens ADPIATP carrier protein (ANT-2) gene, complete cds Page 206 of 209 Table 4 Single Econ Probes Expressed in HBL100 Probe Exon Most Similar SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Top Hit ID NO: Signal BLAST E No. Source Value 4518 9508 14490 0. 94 O. OE+00 L78810. 1 NT Homo sapiens ADP/ATP carrier protein (ANT-2) gene, complete cds 4518 9508 14491 0.94 0. OE+00 L78810. 1 NT Homo sapiens ADP/ATP carrier protein (ANT-2) gene, complete cds 4519 9509 14492 1.58 0.0E+00 AB028970. 1 NT Homo sapiens mRNA for KIAA1047 protein, partial cds 519 9509 14493 1.58 0.0E+00 AB028970. 1 NT Homo sapiens mRNA for KIAA1047 protein, partial cds 4524 9514 14499 3. 45 O. OE+00 Y18890. 1 NT Human endogenous retrovirus type K (HERV-K), gag, pol and env genes 4530 9520 14507 1. 32 O. OE+00 BE081527. 1 EST_HUMAN QV2-BT0635-160400-142-h05 BT0635 Homo sapiens cDNA 4531 9521 14508 1.01 0. OE+00 AA418246. 1 EST HUMAIN zv96b07. s1 SoaresNhHMPuS1 Homo sapiens mRNAclone IMAGE : 767605 3' Homo sapiens truncated tenascin XB (TNXB) gene, partial cds and TNXA gene recombination breakpoint 4536 9526 2. 02 O. OE+00 AF086641. 1 NT region 4541 9530 14517 2.53 0. OE+00 AB037820. 1 NT Homo sapiens mRNA for KIAA1399 protein, partial cds 4541 9530 14518 2.53 0. OE+00 AB037820. 1 NT Homo sapiens mRNA for KIAA1399 protein, partial cds 4542 9531 14519 2. 25 O. OE+00 M74099. 1 NT Human displacement protein (CCAAT) mRNA 4544 9533 14521 2. 86 O. OE+00 6453812 NT Homo sapiens butyrophilin, subfamily 2, member A2 (BTN2A2), mRNA 4544 9533 14522 2. 86 O. OE+00 6453812 NT Homo sapiens butyrophilin, subfamily 2, member A2 (BTN2A2), mRNA 4549 9537 1.17 0. OE+00 BE278730. 1 EST_HUMAN 601158935F1 NIH_MGC_21 Homo sapiens mRNA clone IMAGE : 3505521 5' 4570 9555814546 1. 12 O. OE+00 5729817 NT Homo sapiens ecoltropic viral integration site 2B (EVI2B), mRNA 4570 9558 14547 1. 12 O. OE+00 5729817 NT Homo sapiens ecotropic viral integration site 2B (EVI2B), mRNA 4575 9563 14551 5. 79 O. OE+00 M80902. 1 NT Human AHNAK nucleoprotein mRNA, 5'end 4578 9566 14554 2. 21 O. OE+00 M69197. 1 NT Human haptoglobin and haptoglobin-related protein (HP and HPR) genes, complete cds 4578 9566 14555 2.21 0. OE+00 M69197. 1 NT Human haptoglobin and haptoglobin-related protein (HP and HPR) genes, complete cds 4581 9569 14558 1. 86 O. OE+00 AF184110. 1 NT Homo sapiens cyclophilin-related protein (NKTR) gene, complete cds 4582 9570 14559 0.68 0.0E+00 7662479 NT Homo sapiens KIAA1084 protein (KIAA1084), mRNA 4583 9571 14560 2. 97 O. OE+00 7662181 NT Homo sapiens KIAA0563 gene product (KIAA0563), mRNA 4590 9578 14568 1. 07 O. OE+00 U07563. 1 NT Human proto-oncogene tyrosine-protein kinase (ABL) gene, exon 1a and exons 2-10, complete cds 4594 9582 14572 1.24 0. OE+00 AL096857.1 NT Novel human mRNA from chromosome 1, which has similarities to BAT2 genes 4599 9587 1.15 0. OE+00 X58467. 1 NT Human CYP2D7AP pseudogene for cytochrome P450 2D6 4615 9600 14586 1. 22 O. OE+00 AF026801. 1 NT Homo sapiens alpha-3 type IX collagen (COL9A3) gene, promoter region, and exons 1-26 4617 9602 14588 1.69 0. OE+00 6677700 NT Homo sapiens G-protein coupled receptor (RE2), mRNA 4617 96F2 14589 1. 69 O. OE+00 6677700 NT Homo sapiens G-protein coupled receptor (RE2), mRNA 4618 9603 14590 0. 75 O. OE+00 7019320 NT Homo sapiens proteinx0008 (AD013), mRNA 4618 9603 14591 0. 75 O. OE+00 7019320 NT Homo sapiens proteinx0008 (AD013), mRNA 4641 9626 14618 1.65 0. OE+00 AW444637. 1 EST_HUMAN UI-H-B13-ajw-c-04-0-UI.s1 NCI_CGAP_Sub5 Homo sapiens cDNA clone IMAGE : 2733294 3' 4647 9632 14627 1.01 0.0E+00 AF303134.1 NT Homo sapiens aldehyde dehydrogenase 12 (ALDH12) mRNA, complete cds Page 207 of 209 Table 4 Single Exon Probes Expressed in HBL100 Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top Hit Acession SEQ ID SEQ ID Database Top Hit Descriptor ID NO: Signal BLAST E No. NO: NO: Source. Value 46491 9634 1. 44 O. OE+00 AF083242. 1 NT Homo sapiens HSPC024-iso mRNA, complete cds 4688 9673 14655 0. 66 O. OE+00 J00191. 1 NT Human mHC class transplantation antigen (hala) gene 46881 9673 14656 0. 66 O. 0#+00 J00191. 1 NT Human MHC ciass (transplantation antigen (hla) gene Homo sapiens glutathione S-transferase theta 2 (GSTT2) and glutathione S-transferase theta 1 (GSTT1) 4694 9679 6. 96 O. OE+00 AF240786, 1 NT genes, complete cds 4698 9683 14666 2. 39 O. 0E+00 X87205. 1 NT M. fascicularis mRNA for metalloprotease4ike, disintegrin-like protein, va 4700 9685 14668 1. 77 O. 0E+00 AF084479. 1 NT Homo sapiens Williams-Beuren syndrome deletion transcript 9 (WBSCR9) mRNA, complete cds 4701 9686 14669 1. 47 O. 0E+00 AF097416. 1 NT Mus musculus zinc finger transcription factor Kaiso mRNA, complete cds 4702 9687 14670 4. 25 O. OE+00 4503766 NT Homo sapiens fragile X mental retardation 2 (FMR2) mRNA 4704 9689 14672 13. 59 O. 0E+00 4885048 NT Homo sapiens actin, alpha, cardiac muscl (ACTC), MARNA 4706 9691 14674 2. 35 O. OE+00 8922180 NT Homo sapiens hypothetical protein DKFZp762E1312 (DKFZp762E1312), mRNA 4709 9694 14678 7. 7 O. OE+00 8923080 NT Homo sapiens hypothetical protein FLJ20073 (FLJ20073), mRNA 4712 9697 14681 2. 67 O. OE+00 7661979 NT Homo sapiens KIAA0187 gene product (KIAA0187), mRNA Human Tcr-C-delta gene, exons 1-4 ; Tcr=V-delta gene, exons 1-2 ; T-cell receptor alpha (Tcr-alpha) gene, J1- 4713 9698 14682 1. 91 O. 0E+00 M94081. 1 NT J61 segments ; and Tcr-C-alpha gene, exons 1-4 Human Tcr-C-detta gene, exons 1-4 ; Tcr-V-delta gene, exons 1-2 ; T-cell receptor alpha (Tcr-alpha) gene, J1- 4713 9698 14683 1. 91 O. 0E+00 M94081. 1 NT J61 segments ; and Tcr-C-afpha gene, exons 1-4 4715 9700 14685 1.8 O. 0E+00 X94628. 1 NT H. sapiens MeCP-2 gene 4715 9700 14686 1.8 0.0E+00 X94628. 1 NT H. sapiens MeCP-2 gene 4718 9703 14689 1. 97 O. 0E+00 AL163280. 2 NT Homo sapiens chromosome 21 segment HS21 C080 Homo sapiens TATA box binding protein (TBP)-associated factor, RNA polymerase II, I, 28kD (TAF21) 4727 9712 14697 1. 27 O. OE+00 5032150 NT mRNA 4732 9717 147023. 92 O. 0E+00 X92841. 1 NT H. sapiens MICA gene 4734 9719 14704 1. 68 O. OE+00 4585642 NT Homo sapiens zinc finger protein (KIAA0412) mRNA 4735 9720 14705 1.08 O. 0E+00 AB037864. 1 NT Homo sapiens mRNA for KIAA1443 protein, partial cds 4736 9721 14706 0. 92 O. 0E+00 AB014533. 1 NT Homo sapiens mRNA for KIAA0633 protein, partial cds 4737 9722 14707 2. 29 O. 0E+00 6677648 NT Mus musculus zinc finger protein interacting with K prote7in I (Zikl), MRNA 4738 9723 14708 1. 04 O. 0E+00 5174560 NT Homo sapiens meningioma expressed antigen 6 (coiled-coil proline rich) (MGEA6), mRNA 4740 9725 14710 1. 45 O. OE+00 4758199 NT Homo sapiens desmoplakin (DPI, DPIl) (DSP) mRNA 4744 9729 14716 38. 01 O. OE+00 AF055068. 1 NT Homo sapiens MHC class 1 region 4746 9731 2.39 O. 0E+00 4505508 NT Homo sapiens opioid receptor, delta 1 (OPRD1 ) mRNA 4747 9732 14719 2.63 O. 0E+00 AF091711.1 NT Homo sapiens splice variant AKAP350 mRNA, partial cds 4760 9744 14730 1.02 O. 0E+00 D63562.1 NT Homo sapiens COL4A6 gene for a6 (IV) collagen, exon 44 and partial cds Page 208 of 209 Table 4 Sinale Eacon Probes Exoressed in HBL100 Most Similar Probe Exon Top Hit ORF SEQ Expression (Top) Hit Top HIt Acession SEQ ID SEQ ID ID NO: Signal BLAST E No. Source NO: NO: Source Value Homo sapiens famesyl diphosphate synthase (famesyl pyrophosphate synthetase, 4762 9746 14732 1. 0E+00 4503684 NT dimethylallyltranstransferase, geranyltranstransferase) 96 O. qh68d08.x1 Soares_liver_spleen_1NFLS_S1 (FDPS) mRNA cDNA clone IMAGE Homo sapiens AI249062. : 1849839 3'similar to 4764 9748 14735 1. 13 O. OE+00 ATPN_BOVIN Q28852 1 EST HUMAIN SW : qh68d08.x1 Soares_fetal_liver_spleen_1NFLS_S1 ATP SYNTHASE G CHAIN, MITOCHONDRIAL ; cDNA clone IMAGE Homo sapiens 3' similar : 1849839 AI249062. to 4764 9748 14736 1. 13 O. OE+00 ATPN BOVIN @ 1 EST HUMAIN SW : qm15f05.x1 NCl_CGAP_Lu5 Q28852 ATP SYNTHASE G CHAIN, MITOCHONDRIAL ; cDNA clone IMAGE Homo sapiens 188192 3' similar : 4768 to TR : Q61632 Q61632 AI291129. 9752 1. 07 O. OE+00 EST_HUMAN 1 KIAA0806 EN-2/LACZ FUSION PROTEIN ; 4795 9779 14762 1. 2 O. OE+00 AL163284. 2 NT Homo sapiens chromosome 21 segment HS21 C084 4801 9785 14767 1. 02 O. OE+00 7662319 NT Homo sapiens (KIAA0806), mRNA gene product 0.86 4808 9792 14774 EST_HUMAN zq66b06.s1 O. OE+00 AA205437. 1 neuropithelium (#937231) Stratagene cDNA clone IMAGE Homo sapiens 4825 9809 2. : 646547 3' 4813 9797 6. 67 O. OE+00 U14967. 1 NT Human ribosomal protein L21 mRNA, complete cds 4823 9807 14789 1. 6 O. OE+00 M10976. 1 NT Human endogenous retroviral DNA (4-1), complete retroviral segment 0E+00 99 O. EST_HUMAN 601303729F1 NIH_MGC_21 BE408863. 1 cDNA clone IMAGE Homo sapiens 0E+00 : 3638118 5' 4829 9813 14794 3. 55 O. desmoplakin (DPI, DPII) 4758199 NT Homo sapiens FLJ20477 (DSP) mRNA 4836 9820 14797 2. 16 O. OE+00 AB028966. 1 NT Homo sapiens mRNA for KIAA1043 protein, partial cds 4848 9830 14804 2. 53 O. OE+00 8923441 NT Homo sapiens hypothetical protein (FLJ20477), mRNA (FLJ20477), mRNA 4848 9830 14805 2. 53 O. OE+00 8923441 NT Homo sapiens hypothetical protein FLJ20477 no14g09.s1. NCl_CGAP_Phe1 cDNA clone IMAGE Homo sapiens 1100704 3' similar : 486 to TR : E239140 14815 1 9843 no14g09.s1 NCl_CGAP_Phe1 O. OE+00 AA601246. 1 EST HUMAIN E239140 SPALT PROTEIN ; cDNA clone IMAGE Homo sapiens 1100704 3' similar : 14816 1 0. to TR : E239140 4862 9843 EST_HUMAN OE+00 AA601246. 1 PROTEIN E239140 SPALT no14g09.s1 NCl_CGAP Phe1 ; cDNA clone IMAGE Homo sapiens 1100704 3' similar : HSPC114 mRNA, to TR : E239140 4862 9843 14817 1 O. OE+00 AA601246. 1 EST HUMAIN E239140 SPALT PROTEIN ; 4864 9844 14818 1. 59 O. OE+00 AF161463. 1 NT Homo sapiens 4864 9844 14819 complete cds HSPC114 mRNA, compete 1. 59 O. OE+00 AF161463 I NT Homo sapiens 1 NT cds 48675275 10289 0. 81 O. OE+00 AF195658. ubipuitin0protein ligase Homo sapiens DNA mismatch repair protein (MLH3) gene, complete cds 4870 9849 0. 92 O. OE+00 4758225 NT Homo sapiens E2F transcription factor 2 (E2F2) MARNA 4881 9860 14831 1. 28 O. OE+00 AF016705. 1 NT Homo sapiens E6-AP 3 (UBE3A) gene, exon 2 NT 4883 9862 14833 1. 27 O. OE+00 U53588. 1 NT Homo sapiens MHC class 1 region 4887 9866 1. 11 O. OE+00 AL163209. HS21C009 Homo sapiens chromosome 21 segment gammma-cytoplasmic actin 48 27. 33. O. OE+00 D50657. 1 NT Homo sapiens 9892 14866 (ACTGP3) pseudogene 4913 NT Bacillus amyloiquefaciens sacB 3. 61 O. OE+00 X52988. 1 9904 gene for levansucrase (EC 2. 4. 1. 10) 4927 2.48 0E+00 AF2725663.1 NT 14882 Homo sapiens gephyrin mRNA, complete cds Page 209 of 209 Table 4 Single Exon Probes Expressed in HBL100 Most Similar Probel Exon Top HIt SEQ ID SEQ ID ORF SEQ Expression (Top) Hit Top Hit Acession Database Top Hit Descriptor NO : NO : ID NO : Signal BLAST E No. Source Value 4930, 9907 14884 1. 13 O. OE+00 5454153 NT Homo sapiens cyclophilin (USA-CYP) mRNA 4936 99113 1481 0. 99 O. OE+00 AA683268. 1 EST_HUMAN ae92b04.s1 Stratagene schizo brain S11 Homo sapiens cDNA clone IMAGE : 1020367 3' 4951 9928 14906 0.05 0E+00 4557362 NT Homo sapiens PR domain containing 1, with ZNF domain (PRDM1) mRNA 4957) 9934 14912 0. 72 O. OE+00 Y08032.1 NT Human endogenous retrovirus-K, LTR U5 and gag gene 4965 9942 14919 0. 92 O. OE+00 AF124250. 1 NT Homo sapiens SH2-containing protein Nsp2 mRNA, compete cds 4980 9955 14933 0.92 0E+00 7662421 NT Homo sapiens KIAA0971 protein (KIAA0971), mRNA 4981 9956 14934 0.67 0E+00 4826795 NT Homo sapiens potassium vdtage-gated channel, Isk-related family, member 2 (KCNE2) mRNA 4990 9963 14940 1. 07 O. OE+00 AF108830. 1 NT Homo sapiens serine-threonine protein Kinase (MNBH) mRNA, complete cds 49901 9963 14941 1. 07 O. OE+00 AF108830. 1 NT Homo sapiens serine-threonine protein kinase (MNBH) mRNA, complete cds 5009 9980 14955 1. 27 O. OE+00 7657203 NT Homo sapiens acidic 82 kDa protein mRNA (HSU 15552), mRNA 5034 10005 14976 1.17 0. OE+00 4758021 NT Homo sapiens coagulation factor C (Limulus polyphemus) homology (COCH), mRNA 5049 10020 14989 0. 99 O. OE+00 6677700 NT Homo sapiens G-protein coupled recptor (RE2), mRNA 5049 10020 14990 0. 99 O. OE+00 6877700 NT Homo sapiens G-protein couled receptor (RE2), mRNA 5053 10024 14993 0. 74 O. OE+00 7657336 NT Homo sapiens mute (E. coli) homolog 3 (MLH3), mRNA 5068 10037 15004 0. 75 O. OE+00 AL044081. 1 EST_HUMAN DKFZp434L2428_r1 434 (synonym : htes3) Homo sapiens cDNA clone DKFZp434L2428 5'