Title:
Hybrid picornaviruses expressing chlamydial epitops
Document Type and Number:
WIPO Patent Application WO1994026900
Kind Code:
A3
Abstract:
Hybrid picornaviruses expressing chlamydial epitopes from the major outer membrane protein of Chlamydia trachomatis in a functional form are described. The hybrid viruses grow to high titre in cell culture and when administered to mammals induce an immune response against both the picornavirus and C. trachomatis. The antisera from immunised mammals neutralised both homotypic and heterotypic serovars of C. trachomatis. The hybrid picornaviruses have utility as vaccines and as tools for the generation of immunological reagents. Methods for modifying surface exposed loops of known sequences to produce hybrid proteins are described.
More Like This:
Inventors:
MURDIN ANDREW DAVID (CA)
CALDWELL HARLAN DELANO (US)
KLEIN MICHEL HENRI (CA)
OOMEN RAYMOND PETER (CA)
CALDWELL HARLAN DELANO (US)
KLEIN MICHEL HENRI (CA)
OOMEN RAYMOND PETER (CA)
Application Number:
PCT/CA1994/000262
Publication Date:
February 16, 1995
Filing Date:
May 12, 1994
Export Citation:
Assignee:
CONNAUGHT LAB (CA)
MURDIN ANDREW DAVID (CA)
CALDWELL HARLAN DELANO (US)
KLEIN MICHEL HENRI (CA)
OOMEN RAYMOND PETER (CA)
MURDIN ANDREW DAVID (CA)
CALDWELL HARLAN DELANO (US)
KLEIN MICHEL HENRI (CA)
OOMEN RAYMOND PETER (CA)
International Classes:
C07K14/295; C12N15/31; C12N15/32; C12N15/62; A61K39/00; (IPC1-7): C12N15/31; C12N7/00; C12N15/86; A61K39/118; C12P21/08; A61K39/13; G01N35/571; C12N15/62
Domestic Patent References:
| WO1990015145A2 | 1990-12-13 | |||
| WO1992014489A1 | 1992-09-03 |
Other References:
BAEHR, W. ET AL.: "Mapping antigenic domains expressed by Chlamydia trachomatis major outer membrane protein genes", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, vol. 85, June 1988 (1988-06-01), WASHINGTON US, pages 4000 - 4004
SU, H. & CALDWELL, H.: "Immunogenicity of a chimeric peptide corresponding to T helper and B cell epitopes of the Chlamydia trachomatis major outer membrane protein", THE JOURNAL OF EXPERIMENTAL MEDICINE, vol. 175, no. 1, 1 January 1992 (1992-01-01), pages 227 - 235
MURDIN, A. ET AL.: "The effects of site and mode of expression of a heterologous antigenic determinant on the properties of poliovirus hybrids", MICROBIAL PATHOGENESIS, vol. 10, no. 1, January 1991 (1991-01-01), pages 27 - 37
MURDIN, A. ET AL.: "THE EFFECT OF SITE AND MODE OF EXPRESSION OF A HETEROLOGOUS ANTIGENIC DETERMINANT ON THE PROPERTIES OF POLIOVIRUS HYBRIDS.", MICROB PATHOG 10 (1). JANUARY 1991. 27-38
QUINN, T.C. :'Sexually transmitted diseases', 1992, RAVEN PRESS, Ltd., New York; pages 57-84 MORRISON, R. et al.:
MURDIN, A. ET AL: "A POLIOVIRUS HYBRID EXPRESSING A NEUTRALIZATION EPITOPE FROM THE MAJOR OUTER MEMBRANE PROTEIN OF CHLAMYDIA -TRACHOMATIS IS HIGHLY IMMUNOGENIC.", INFECT IMMUN 61 (10). 1993. 4406-4414
SU, H. & CALDWELL, H.: "Immunogenicity of a chimeric peptide corresponding to T helper and B cell epitopes of the Chlamydia trachomatis major outer membrane protein", THE JOURNAL OF EXPERIMENTAL MEDICINE, vol. 175, no. 1, 1 January 1992 (1992-01-01), pages 227 - 235
MURDIN, A. ET AL.: "The effects of site and mode of expression of a heterologous antigenic determinant on the properties of poliovirus hybrids", MICROBIAL PATHOGENESIS, vol. 10, no. 1, January 1991 (1991-01-01), pages 27 - 37
MURDIN, A. ET AL.: "THE EFFECT OF SITE AND MODE OF EXPRESSION OF A HETEROLOGOUS ANTIGENIC DETERMINANT ON THE PROPERTIES OF POLIOVIRUS HYBRIDS.", MICROB PATHOG 10 (1). JANUARY 1991. 27-38
QUINN, T.C. :'Sexually transmitted diseases', 1992, RAVEN PRESS, Ltd., New York; pages 57-84 MORRISON, R. et al.:
MURDIN, A. ET AL: "A POLIOVIRUS HYBRID EXPRESSING A NEUTRALIZATION EPITOPE FROM THE MAJOR OUTER MEMBRANE PROTEIN OF CHLAMYDIA -TRACHOMATIS IS HIGHLY IMMUNOGENIC.", INFECT IMMUN 61 (10). 1993. 4406-4414
Download PDF: