Title:
INSULIN-PRODUCING CELLS AND METHOD OF CONSTRUCTING THE SAME
Document Type and Number:
WIPO Patent Application WO/2004/104195
Kind Code:
A1
Abstract:
It is intended to provide insulin-producing cells, which can be constructed with the use of liver-origin cells obtained from a normal liver tissue as the starting material, and a method of constructing the same. By culturing small-sized hepatocytes which are undifferentiated stem cells under definite conditions, these cells can be differentiated into insulin-producing cells. Further, the stimulus-response extracellular insulin secretion system of the cells can be improved by transferring a Pdx-1 gene into the cells. The insulin-producing/secreting cells thus obtained are physiologically closely similar to islet β cells and usable in treating or studying diabetes, etc. In particular, it is expected that these cells would be usable as donor cells in cell transplantation therapy for diabetes.
Inventors:
NAKAZIMA NATSUKI (JP)
SAKURAI TOMONORI (JP)
TABATA YASUHIKO (JP)
SAKURAI TOMONORI (JP)
TABATA YASUHIKO (JP)
Application Number:
PCT/JP2003/014681
Publication Date:
December 02, 2004
Filing Date:
November 18, 2003
Export Citation:
Assignee:
JAPAN SCIENCE & TECH AGENCY (JP)
NAKAZIMA NATSUKI (JP)
SAKURAI TOMONORI (JP)
TABATA YASUHIKO (JP)
NAKAZIMA NATSUKI (JP)
SAKURAI TOMONORI (JP)
TABATA YASUHIKO (JP)
International Classes:
C12N15/09; C07K14/47; C07K14/575; C07K14/62; C12N5/071; C12N5/10; (IPC1-7): C12N15/09; C07K14/575; C07K14/62; C12N5/10
Foreign References:
| JPH10179148A | 1998-07-07 |
Other References:
YANG L. et al., "In vitro trans-differentiation of adult hepatic stem cells into pancreatic endocrine hormone-producing cells.," Proc. Natl. Acad.Sci,USA, 11 June, 2002, Vol. 99, No. 12, pages 8078 - 8083
Jun'ichi MIYAZAKI, "ES Saibo Kara Sui beta Saibo eno Bunka Yudo wa kanoka?", Diabetes J., (2000), Vol. 29, No. 3, pages 114 - 115
Tekeki OGATA et al., 'Seikatsu Shukanbyo Bunshi Mechanism to Chiryo V Tonyobyo Kitai Sareru Chiryoho 2 15 Sui no Saisei', Mol. Med., (2001), Vol. 38, special extra issue., pages 234 - 239
Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia., Nat.Med., (2000), Vol. 6, No. 5, pages 568 - 572
MIYATSUKA, T. et al., "Ectopically expressed PDX-1 in liver initiates endocrine andexocerine pancreas differentiation but causes dysmorphogenesis," Biochem. Biophys.Res. Commun., 24 October, 2003, Vol. 310, No.3, pages 1017 - 1025
Shozo ANSHIN'IN, "Teikessei Baichi o Mochiiru Keishitu Tenkan Shiken ni yoru Jaku Hatsugansei Busshitsu no Kenshutsu," The Japanese Environmental Mutagen Society Dai 28 Kai Taikai Program Yoshishu, (1999), page 139, p-109
Jun'ichi MIYAZAKI, "ES Saibo Kara Sui beta Saibo eno Bunka Yudo wa kanoka?", Diabetes J., (2000), Vol. 29, No. 3, pages 114 - 115
Tekeki OGATA et al., 'Seikatsu Shukanbyo Bunshi Mechanism to Chiryo V Tonyobyo Kitai Sareru Chiryoho 2 15 Sui no Saisei', Mol. Med., (2001), Vol. 38, special extra issue., pages 234 - 239
Pancreatic and duodenal homeobox gene 1 induces expression of insulin genes in liver and ameliorates streptozotocin-induced hyperglycemia., Nat.Med., (2000), Vol. 6, No. 5, pages 568 - 572
MIYATSUKA, T. et al., "Ectopically expressed PDX-1 in liver initiates endocrine andexocerine pancreas differentiation but causes dysmorphogenesis," Biochem. Biophys.Res. Commun., 24 October, 2003, Vol. 310, No.3, pages 1017 - 1025
Shozo ANSHIN'IN, "Teikessei Baichi o Mochiiru Keishitu Tenkan Shiken ni yoru Jaku Hatsugansei Busshitsu no Kenshutsu," The Japanese Environmental Mutagen Society Dai 28 Kai Taikai Program Yoshishu, (1999), page 139, p-109
Attorney, Agent or Firm:
Tsuburaya, Toru (Osaka Ekimae Dai-3 Bldg. 1-3, Umeda 1-chome, Kita-k, Osaka-shi Osaka, JP)
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