Title:
INTRACELLULAR DELIVERY OF SMALL MOLECULES PROTEINS AND NUCLEIC ACIDS
Document Type and Number:
WIPO Patent Application WO2004078933
Kind Code:
A3
Abstract:
An amino acid sequence Arg-Lys-Met-Leu-Lys-Ser-Thr-Arg-Arg-Gln-Arg-Arg (SEQ ID NO: 1) functions as a protein transduction domain (PTD) and is capable of delivering small molecules, proteins, and nucleic acids to an intracellular compartment of a cell. An amino terminal lysine linker improves the efficiency of the PTD. A nuclear localization signal can be used to target the PTD to a cell's nucleus. The PTD can be used in PTD-cargo moiety complexes that can reversibly immortalize cells and increase cell viability in culture.
Inventors:
KARAS MICHAEL (US)
Application Number:
PCT/US2004/006445
Publication Date:
May 06, 2005
Filing Date:
March 04, 2004
Export Citation:
Assignee:
BIOWHITTAKER TECHNOLOGIES INC (US)
KARAS MICHAEL (US)
KARAS MICHAEL (US)
International Classes:
A61K38/10; A61K38/14; A61K38/16; A61K47/48; C07H19/00; C07H21/04; C07K7/08; C07K14/16; C12N15/63; C12P19/34; C12P21/06; C12N; (IPC1-7): A61K38/10; C07H19/00; C07K7/08; C12P21/06; C12P19/34; C12N15/63
Other References:
FAWELL ET AL: "Tat-mediated delivery of the heterologous proteins into cells", PROC. NATL. ACAD. SCI., vol. 91, no. 2, January 1994 (1994-01-01), USA, pages 664 - 668, XP002936154
DIETZ ET AL: "Inhibition of Neuronal Apoptosis in Vitro and in Vivo Using TAT-mediated Protein Transduction", MOLECULAR AND CELLULAR NEUROSCIENCE, vol. 21, December 2002 (2002-12-01), pages 29 - 37, XP002985908
DIETZ ET AL: "Inhibition of Neuronal Apoptosis in Vitro and in Vivo Using TAT-mediated Protein Transduction", MOLECULAR AND CELLULAR NEUROSCIENCE, vol. 21, December 2002 (2002-12-01), pages 29 - 37, XP002985908
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