Title:
MALTOBIONIC ACID PREPARATION METHOD USING ENZYMATIC OXIDATION
Document Type and Number:
WIPO Patent Application WO/2022/265185
Kind Code:
A1
Abstract:
A maltobionic acid preparation method according to the present invention comprises the steps of: preparing a maltose-containing saccharide solution having a solid matter concentration of 15-25 Brix and containing 90 weight% or higher of maltose relative to the total weight of solid saccharide; and adding 4.0-5.0% (v/w) of carbohydrate oxidase and 0.1-0.8% (v/w) of catalase, relative to the weight of solid saccharide, to the maltose-containing saccharide solution, and performing an enzymatic oxidation reaction for 15-30 hours at a temperature of 22-35℃, at a pH of 6-8, at an aeration rate of 0.9-1.2 vvm, and at a stirring rate of 150-300 rpm, to obtain a reaction product-containing solution. By using the preparation method according to the present invention, the conversion rate of the maltose to a maltobionic acid may be 99% or higher, and a high-purity maltobionic acid having a purity of 95% or higher and in a powder form may be produced without using separate chromatographic separation.
Inventors:
KIM SEON HONG (KR)
KIM HYOUNG SUB (KR)
HAM CHOONG HYUN (KR)
KIM HAK JUN (KR)
KIM HYOUNG SUB (KR)
HAM CHOONG HYUN (KR)
KIM HAK JUN (KR)
Application Number:
PCT/KR2022/003186
Publication Date:
December 22, 2022
Filing Date:
March 07, 2022
Export Citation:
Assignee:
DAESANG CORP (KR)
International Classes:
C12P19/04; C12N9/04; C12N9/08
Foreign References:
| KR20200120722A | 2020-10-21 | |||
| KR20160144608A | 2016-12-19 | |||
| KR102090063B1 | 2020-03-17 | |||
| JP2003503436A | 2003-01-28 | |||
| KR20210014172A | 2021-02-08 |
Attorney, Agent or Firm:
LIM, Sang Yeob (KR)
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