METHOD FOR DETECTING TARGET PARTICLES

Title:

METHOD FOR DETECTING TARGET PARTICLES

Document Type and Number:

WIPO Patent Application WO/2013/031365

Kind Code:

A1

Abstract:

This method for detecting target particles includes: (a) a step in which a solution is prepared that contains the target particles, a light-emitting probe that bonds with the target particles, and separation/recovery particles, or contains the target particles bonded to the light-emitting probe, the light-emitting probe itself, and the separation/recovery particles, and next, in the solution a composite is formed that comprises the target particles, the light-emitting probe, and the separation/recovery particles; (b) a step following the step (a) and in which separation of the liquids and solids causes the separation/recovery particles to be recovered, and a sample solution containing the separation/recovery particles is prepared; and (c) a step in which the number of molecules of the composite present in the sample solution is calculated using a scanning molecule counting method. Therein, the separation/recovery particles bond with an aggregate comprising the target particles and the light-emitting probe.

Inventors:

HANASHI TAKUYA (JP)

Application Number:

PCT/JP2012/066576

Publication Date:

March 07, 2013

Filing Date:

June 28, 2012

Export Citation:

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Assignee:

OLYMPUS CORP (JP)
HANASHI TAKUYA (JP)

International Classes:

G01N21/64

Domestic Patent References:

WO2011108369A1	2011-09-09
WO2012014778A1	2012-02-02

Foreign References:

JPH04501956A	1992-04-09
JP2008058285A	2008-03-13
JP2011508219A	2011-03-10
JP2008116440A	2008-05-22
JP2011002415A	2011-01-06
JP2010190730A	2010-09-02

Other References: