Title:
METHOD FOR HIGHLY SENSITIVE DETECTION OF PROTEIN-PROTEIN INTERACTION
Document Type and Number:
WIPO Patent Application WO/2010/137717
Kind Code:
A1
Abstract:
Disclosed is an assay system which utilizes a split luciferase and has a significantly high sensitivity. In an embodiment of the assay system, for the purpose of detecting the binding between a first protein and a second protein both having a binding ability to each other, a first fusion protein of the first protein and a peptide having an amino acid sequence depicted in SEQ ID NO: 1 is prepared, a second fusion protein of the second protein and a peptide having an amino acid sequence selected from the group consisting of the sequences depicted in SEQ ID NO: 2 to SEQ IS NO: 6 is prepared, the first fusion protein and the second fusion protein are allowed to interact with each other to form a complex, and subsequently the luminescent activity of the complex is determined.
Inventors:
OZAWA TAKEAKI (JP)
MISAWA NAOMI (JP)
MIURA KENJI (JP)
OKAMOTO TASUKU (JP)
NISHII SHIGEAKI (JP)
MASUDA KENJI (JP)
MISAWA NAOMI (JP)
MIURA KENJI (JP)
OKAMOTO TASUKU (JP)
NISHII SHIGEAKI (JP)
MASUDA KENJI (JP)
Application Number:
PCT/JP2010/059160
Publication Date:
December 02, 2010
Filing Date:
May 28, 2010
Export Citation:
Assignee:
UNIV TOKYO (JP)
PROBEX INC (JP)
TOYO BOSEKI (JP)
OZAWA TAKEAKI (JP)
MISAWA NAOMI (JP)
MIURA KENJI (JP)
OKAMOTO TASUKU (JP)
NISHII SHIGEAKI (JP)
MASUDA KENJI (JP)
PROBEX INC (JP)
TOYO BOSEKI (JP)
OZAWA TAKEAKI (JP)
MISAWA NAOMI (JP)
MIURA KENJI (JP)
OKAMOTO TASUKU (JP)
NISHII SHIGEAKI (JP)
MASUDA KENJI (JP)
International Classes:
C12N15/09; C07K14/47; C07K19/00; C12N9/02; C12Q1/26; G01N21/76; G01N33/566
Domestic Patent References:
| WO2007027919A2 | 2007-03-08 |
Foreign References:
| JP2007159567A | 2007-06-28 | |||
| JP2008289475A | 2008-12-04 |
Other References:
KIM SB. ET AL.: "High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase", PROC. NATL. ACAD. SCI. U.S.A., vol. 101, no. 32, 2004, pages 11542 - 11547, XP002479375
LUKER KE. ET AL.: "Imaging CXCR4 signaling with firefly luciferase complementation", ANAL. CHEM., vol. 80, no. 14, 2008, pages 5565 - 5573, XP008148448
KIM, S.B.; OZAWA, T.; WATANABE, S.; UMEZAWA, Y., PROC. NATL. ACAD. SCI. USA., vol. 101, 2004, pages 11542 - 11547
"Molecular cloning, a laboratory manual", 2001, COLD SPRING HARBOR PRESS
"Current Protocols in Molecular Biology", JOHN WILEY & SONS LTD.
See also references of EP 2436764A4
LUKER KE. ET AL.: "Imaging CXCR4 signaling with firefly luciferase complementation", ANAL. CHEM., vol. 80, no. 14, 2008, pages 5565 - 5573, XP008148448
KIM, S.B.; OZAWA, T.; WATANABE, S.; UMEZAWA, Y., PROC. NATL. ACAD. SCI. USA., vol. 101, 2004, pages 11542 - 11547
"Molecular cloning, a laboratory manual", 2001, COLD SPRING HARBOR PRESS
"Current Protocols in Molecular Biology", JOHN WILEY & SONS LTD.
See also references of EP 2436764A4
Attorney, Agent or Firm:
ISSHIKI & CO. (JP)
All international patent business corporation (JP)
All international patent business corporation (JP)
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