Title:
METHOD FOR IDENTIFYING BLOOD CELL HEMOSTASIS
Document Type and Number:
WIPO Patent Application WO2002052275
Kind Code:
A3
Abstract:
The invention relates to a method for identifying blood clotting, in particular, blood clotting that is triggered by the intravascular tissue factor (TF). The inventive method involves the following steps: collecting a whole blood sample; stabilizing the whole blood sample by adding an anticoagulant substance; adding at least one constituent to the extracellular matrix; adding an inhibitor that inhibits contact activation; adding factor VIIa(i), and; detecting the blood clotting using a customary method.
Inventors:
ENGELMANN BERND (US)
SPANNAGL MICHAEL (DE)
BERTLING WOLF (DE)
SPANNAGL MICHAEL (DE)
BERTLING WOLF (DE)
Application Number:
PCT/DE2001/004815
Publication Date:
August 08, 2002
Filing Date:
December 19, 2001
Export Citation:
Assignee:
NOVEMBER AG MOLEKULARE MEDIZIN (DE)
ENGELMANN BERND (US)
SPANNAGL MICHAEL (DE)
BERTLING WOLF (DE)
ENGELMANN BERND (US)
SPANNAGL MICHAEL (DE)
BERTLING WOLF (DE)
International Classes:
C12Q1/56; G01N33/86; (IPC1-7): G01N33/86
Domestic Patent References:
| WO1999003498A1 | 1999-01-28 |
Foreign References:
| US5472850A | 1995-12-05 |
Other References:
GIESEN P L A ET AL: "BLOOD-BORNE TISSUE FACTOR: ANOTHER VIEW OF THROMBOSIS", PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF USA, NATIONAL ACADEMY OF SCIENCE. WASHINGTON, US, vol. 96, March 1999 (1999-03-01), pages 2311 - 2315, XP002939775, ISSN: 0027-8424
RAUCH U ET AL: "Circulating tissue factor and thrombosis.", CURRENT OPINION IN HEMATOLOGY. UNITED STATES SEP 2000, vol. 7, no. 5, September 2000 (2000-09-01), pages 273 - 277, XP008003987, ISSN: 1065-6251
VALENTIN SANNE ET AL: "Inhibition of factor X activation at extracellular matrix of fibroblasts during flow conditions: A comparison between tissue factor pathway inhibitor and inactive factor Vlla.", THROMBOSIS AND HAEMOSTASIS, vol. 74, no. 6, 1995, pages 1478 - 1485, XP008003991, ISSN: 0340-6245
JOHNSON DANIEL J D ET AL: "Crystallization and preliminary X-ray analysis of active site-inhibited human coagulation factor VIIa (des-Gla).", JOURNAL OF STRUCTURAL BIOLOGY, vol. 125, no. 1, March 1999 (1999-03-01), pages 90 - 93, XP001074734, ISSN: 1047-8477
RAUCH U ET AL: "Circulating tissue factor and thrombosis.", CURRENT OPINION IN HEMATOLOGY. UNITED STATES SEP 2000, vol. 7, no. 5, September 2000 (2000-09-01), pages 273 - 277, XP008003987, ISSN: 1065-6251
VALENTIN SANNE ET AL: "Inhibition of factor X activation at extracellular matrix of fibroblasts during flow conditions: A comparison between tissue factor pathway inhibitor and inactive factor Vlla.", THROMBOSIS AND HAEMOSTASIS, vol. 74, no. 6, 1995, pages 1478 - 1485, XP008003991, ISSN: 0340-6245
JOHNSON DANIEL J D ET AL: "Crystallization and preliminary X-ray analysis of active site-inhibited human coagulation factor VIIa (des-Gla).", JOURNAL OF STRUCTURAL BIOLOGY, vol. 125, no. 1, March 1999 (1999-03-01), pages 90 - 93, XP001074734, ISSN: 1047-8477
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