Title:
METHOD FOR INDUCTION OF SKELETAL MUSCLE CELL OR OSTEOCYTE
Document Type and Number:
WIPO Patent Application WO/2010/008100
Kind Code:
A1
Abstract:
Disclosed is a method for inducing the differentiation of a pluripotent stem cell into an osteocyte, a chondrocyte or a skeletal muscle cell in a serum-free culture medium. Specifically disclosed are: a method for culturing a pluripotent stem cell derived from a mammal in a BMP4-containing serum-free culture medium to induce a PDGFRα-positive mesodermal progenitor cell and cause the differentiation of the PDGFRα-positive mesodermal progenitor cell into an osteocyte or a chondrocyte; and a method for producing a skeletal muscle cell, which comprises culturing a pluripotent stem cell in a BMP4-containing serum-free culture medium, and further culturing the pluripotent stem cell in a serum-free culture medium in the absence of BMP4 and in the presence of LiCl to induce the differentiation of the pluripotent stem cell into a PDGFRα-positive skeletal muscle progenitor cell.
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Inventors:
ISOBE KEN-ICHI (JP)
SAKURAI HIDETOSHI (JP)
SAKURAI HIDETOSHI (JP)
Application Number:
PCT/JP2009/063309
Publication Date:
January 21, 2010
Filing Date:
July 17, 2009
Export Citation:
Assignee:
UNIV NAGOYA NAT UNIV CORP (JP)
ISOBE KEN-ICHI (JP)
SAKURAI HIDETOSHI (JP)
ISOBE KEN-ICHI (JP)
SAKURAI HIDETOSHI (JP)
International Classes:
A61L27/00; C12N15/09
Other References:
HIDETOSHI SAKURAI: "ES Saibo no Saisei Iryo eno Oyo", HEISEI 18 NENDO KOSEI RODO KAGAKU KENKYUHI HOJOKIN CHOJU KAGAKU SOGO KENKYU SUISHIN JIGYO KENKVU HOKOKUSHU, 2007, pages 113 - 116
"Dai 26 Kai Annual Meeting of the Molecular Biology Society of Japan, Program-Koen Yoshishu, 25 November, 2003 (25.11.03)", article HIDETOSHI SAKURAI ET AL.: "In Vitoro ES Saibo Bunka-kei o Mochiita, Tanosei Chuhaiyo Zenku Saibo no Bunri Oyobi Saibo Seibutsugakuteki Kaiseki", pages: 891
KOJI IWAMOTO ET AL.: "LiCl wa BMP4 ni yoru C3H10T1/2 no Yudo Nankotsu de noggin no Hatsugen o Yokusei suru", DAI 21 KAI NIPPON NANKOTSU TAISHA GAKKAI PROGRAM'SHOROKUSHU, March 2008 (2008-03-01), pages 88
HIDETOSHI SAKURAI ET AL.: "Mouse ES Saibo o Mochiita Mukessei Jokenka deno 2 Shurui no Enjiku Chuhaiyo Zenku Saibo o Yudo suru System no Kochiku", DAI 81 KAI NIPPON SEIKAGAKUKAI TAIKAI DAI 31 KAI NIPPON BUNSHI SEIBUTSU GAKKAI NENKAI GODO TAIKAI PROGRAM-KEEN YOSHISHU, 1 December 2008 (2008-12-01), pages 1089
"Dai 26 Kai Annual Meeting of the Molecular Biology Society of Japan, Program-Koen Yoshishu, 25 November, 2003 (25.11.03)", article HIDETOSHI SAKURAI ET AL.: "In Vitoro ES Saibo Bunka-kei o Mochiita, Tanosei Chuhaiyo Zenku Saibo no Bunri Oyobi Saibo Seibutsugakuteki Kaiseki", pages: 891
KOJI IWAMOTO ET AL.: "LiCl wa BMP4 ni yoru C3H10T1/2 no Yudo Nankotsu de noggin no Hatsugen o Yokusei suru", DAI 21 KAI NIPPON NANKOTSU TAISHA GAKKAI PROGRAM'SHOROKUSHU, March 2008 (2008-03-01), pages 88
HIDETOSHI SAKURAI ET AL.: "Mouse ES Saibo o Mochiita Mukessei Jokenka deno 2 Shurui no Enjiku Chuhaiyo Zenku Saibo o Yudo suru System no Kochiku", DAI 81 KAI NIPPON SEIKAGAKUKAI TAIKAI DAI 31 KAI NIPPON BUNSHI SEIBUTSU GAKKAI NENKAI GODO TAIKAI PROGRAM-KEEN YOSHISHU, 1 December 2008 (2008-12-01), pages 1089
Attorney, Agent or Firm:
HIRAKI, Yusuke et al. (3-20 Toranomon 4-chome, Minato-k, Tokyo 01, JP)
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