Title:
METHOD AND KIT FOR DETECTING REPLICATION COMPETENT ADENOVIRUSES
Document Type and Number:
WIPO Patent Application WO2004057035
Kind Code:
A3
Abstract:
A method and a kit for detecting the presence or absence of replication competent adenovirus ("RCA") in a sample suspected of being contaminated with said RCA. The method comprises performing a nested polymerase chain reaction ("PCR") to selectively amplify a targeted RCA nucleotide sequence within the sample, wherein the targeted RCA nucleotide sequence is a nucleotide sequence which overlaps at least a portion of E1 and at least a portion of E2. The method comprises the steps of performing a first round of PCR amplification using an outer primer pair flanking a first targeted RCA nucleotide sequence, the outer primer pair consisting of a 5' outer primer and a 3' outer primer, to yield a first round PCR amplification mixture; performing a second round of PCR amplification from the first round PCR amplification mixture using an inner primer pair flanking a second targeted nucleotide sequence internal to the first targeted nucleotide sequence, the inner primer pair consisting of a 5' inner primer and a 3' inner primer, to yield a second round PCR amplification mixture wherein any one of the 5' inner primer and the 3' inner primer may be identical to the 5' outer primer and the 3' outer primer, respectively and analyzing the second round PCR amplification mixture to detect the presence or absence of the second targeted nucleotide sequence wherein the presence of the second targeted nucleotide sequence indicates the presence of the RCA in the sample. The present invention also relates to a kit for assessing the presence or absence of RCA in a sample comprising an outer primer pair flanking a first region overlapping at least a portion of El and at least a portion of E2 and at least one inner primer so that an inner primer pair is formed flanking a second region within the first region. Additionally, the invention relates to compositions of matter for assessing the presence or absence of RCA in a sample.
Inventors:
GAGNON LUC (CA)
MOISSET PIERRE-ALAIN (CA)
LAROSE CLAUDE (CA)
PELOQUIN LUC (CA)
JOLICOEUR PIERRE (CA)
MOISSET PIERRE-ALAIN (CA)
LAROSE CLAUDE (CA)
PELOQUIN LUC (CA)
JOLICOEUR PIERRE (CA)
Application Number:
PCT/CA2003/001952
Publication Date:
September 30, 2004
Filing Date:
December 19, 2003
Export Citation:
Assignee:
QBIOGENE INC (CA)
GAGNON LUC (CA)
MOISSET PIERRE-ALAIN (CA)
LAROSE CLAUDE (CA)
PELOQUIN LUC (CA)
JOLICOEUR PIERRE (CA)
GAGNON LUC (CA)
MOISSET PIERRE-ALAIN (CA)
LAROSE CLAUDE (CA)
PELOQUIN LUC (CA)
JOLICOEUR PIERRE (CA)
International Classes:
C12Q1/70; (IPC1-7): C12Q1/70
Domestic Patent References:
| WO2001044280A2 | 2001-06-21 | |||
| WO1999053047A2 | 1999-10-21 |
Other References:
LOCHMÜLLER H. ET AL.: "Emergence of early region 1-containing replication-competent adenovirus in stocks of replication-defective adenovirus recombinants (detaE1 + deltaE3) during multiple passages in 293 cells", HUMAN GENE THERAPY, vol. 5, December 1994 (1994-12-01), pages 1485 - 1491, XP002972681, ISSN: 1043-0342
CASTIGNOLLES N. ET AL.: "Detection of Adenovirus in the waters of the Seine River estuary by nested-PCR", MOLECULAR AND CELLULAR PROBES, ACADEMIC PRESS, LONDON, GB, vol. 12, no. 3, June 1998 (1998-06-01), pages 175 - 180, XP004450160, ISSN: 0890-8508
KUWANO K. ET AL.: "Detection of group C adenovirus DNA in small-cell lung cancer with the nested polymerase chain reaction", JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, vol. 123, no. 7, 1997, pages 377 - 382, XP002291486, ISSN: 0171-5216
ZHANG W.-W. ET AL.: "Detection of wild-type contamination in a recombinant adenoviral preparation by PCR", BIOTECHNIQUES, vol. 18, no. 3, 1995, pages 444 - 447, XP002291472, ISSN: 0736-6205
DION L.D. ET AL.: "Supernatant rescue assay vs. polymerase chain reaction for detection of wild type adenovirus-contaminating recombinant adenovirus stocks", JOURNAL OF VIROLOGICAL METHODS, AMSTERDAM, NL, vol. 56, 1996, pages 99 - 107, XP002972682, ISSN: 0166-0934
BAYLEY S. ET AL.: "ADENOVIRUS E1A PROTEINS AND TRANSFORMATION (REVIEW)", INTERNATIONAL JOURNAL OF ONCOLOGY, EDITORIAL ACADEMY OF THE INTERNATIONAL JOURNAL OF ONCOLOGY,, GR, vol. 5, no. 3, September 1994 (1994-09-01), pages 425 - 444, XP000877368, ISSN: 1019-6439
PUIG M. ET AL.: "DETECTION OF ADENOVIRUSES AND ENTEROVIRUSES IN POLLUTED WATERS BY NESTED PCR AMPLIFICATION", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 60, no. 8, August 1994 (1994-08-01), pages 2963 - 2970, XP008028844, ISSN: 0099-2240
ZHOU H. ET AL.: "DEVELOPMENT OF A COMPLEMENTING CELL LINE AND A SYSTEM FOR CONSTRUCTION OF ADENOVIRUS VECTORS WITH E1 AND E2A DELETED", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 70, no. 10, 1 October 1996 (1996-10-01), pages 7030 - 7038, XP002059962, ISSN: 0022-538X
CASTIGNOLLES N. ET AL.: "Detection of Adenovirus in the waters of the Seine River estuary by nested-PCR", MOLECULAR AND CELLULAR PROBES, ACADEMIC PRESS, LONDON, GB, vol. 12, no. 3, June 1998 (1998-06-01), pages 175 - 180, XP004450160, ISSN: 0890-8508
KUWANO K. ET AL.: "Detection of group C adenovirus DNA in small-cell lung cancer with the nested polymerase chain reaction", JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, vol. 123, no. 7, 1997, pages 377 - 382, XP002291486, ISSN: 0171-5216
ZHANG W.-W. ET AL.: "Detection of wild-type contamination in a recombinant adenoviral preparation by PCR", BIOTECHNIQUES, vol. 18, no. 3, 1995, pages 444 - 447, XP002291472, ISSN: 0736-6205
DION L.D. ET AL.: "Supernatant rescue assay vs. polymerase chain reaction for detection of wild type adenovirus-contaminating recombinant adenovirus stocks", JOURNAL OF VIROLOGICAL METHODS, AMSTERDAM, NL, vol. 56, 1996, pages 99 - 107, XP002972682, ISSN: 0166-0934
BAYLEY S. ET AL.: "ADENOVIRUS E1A PROTEINS AND TRANSFORMATION (REVIEW)", INTERNATIONAL JOURNAL OF ONCOLOGY, EDITORIAL ACADEMY OF THE INTERNATIONAL JOURNAL OF ONCOLOGY,, GR, vol. 5, no. 3, September 1994 (1994-09-01), pages 425 - 444, XP000877368, ISSN: 1019-6439
PUIG M. ET AL.: "DETECTION OF ADENOVIRUSES AND ENTEROVIRUSES IN POLLUTED WATERS BY NESTED PCR AMPLIFICATION", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 60, no. 8, August 1994 (1994-08-01), pages 2963 - 2970, XP008028844, ISSN: 0099-2240
ZHOU H. ET AL.: "DEVELOPMENT OF A COMPLEMENTING CELL LINE AND A SYSTEM FOR CONSTRUCTION OF ADENOVIRUS VECTORS WITH E1 AND E2A DELETED", JOURNAL OF VIROLOGY, THE AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 70, no. 10, 1 October 1996 (1996-10-01), pages 7030 - 7038, XP002059962, ISSN: 0022-538X
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