Title:
METHOD FOR MANUFACTURING POLYPEPTIDE INCLUDING PROTEIN-SYNTHESIS-INHIBITING PROTEIN TOXIN
Document Type and Number:
WIPO Patent Application WO/2016/068294
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing art for producing a protein-synthesis-inhibiting protein toxin. A method for manufacturing a polypeptide, said method comprising a step in which a cell-free protein synthesizing system that at least includes an initiation factor, an elongation factor, an aminoacyl-tRNA synthetase, a ribosome, an amino acid, a nucleoside triphosphate, and tRNA is used to synthesize a polypeptide from a nucleic acid that codes said polypeptide including an amino acid sequence for a protein-synthesis-inhibiting protein toxin.
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Inventors:
IGUCHI KEITA (JP)
MORIO KENICHIRO (JP)
KANAMORI TAKASHI (JP)
MATSUMOTO RENA (JP)
NAKAMURA MIKIKO (JP)
MORIO KENICHIRO (JP)
KANAMORI TAKASHI (JP)
MATSUMOTO RENA (JP)
NAKAMURA MIKIKO (JP)
Application Number:
PCT/JP2015/080758
Publication Date:
May 06, 2016
Filing Date:
October 30, 2015
Export Citation:
Assignee:
KANEKA CORP (JP)
GENEFRONTIER CORP (JP)
GENEFRONTIER CORP (JP)
International Classes:
C12P21/00; C07K1/02; C07K14/195; C07K14/25; C07K14/38; C07K14/415; C07K16/18; C12N15/09
Foreign References:
| JP2007536905A | 2007-12-20 | |||
| JP2008528633A | 2008-07-31 | |||
| JP2008508903A | 2008-03-27 | |||
| JP2008500830A | 2008-01-17 | |||
| JP2012187049A | 2012-10-04 |
Other References:
FUJII S. ET AL.: "In vitro evolution of alpha- hemolysin using a liposome display.", PROC NATL ACAD SCI USA, vol. 110, no. 42, 2013, pages 16796 - 16801
Attorney, Agent or Firm:
HIRAKI Yusuke et al. (JP)
Yusuke Hiraki (JP)
Yusuke Hiraki (JP)
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