Title:
METHOD OF MANUFACTURING REFERENCE MATERIAL USING PLANT CULTURED CELL LINES
Document Type and Number:
WIPO Patent Application WO/2011/074897
Kind Code:
A2
Abstract:
The present invention relates to a method of manufacturing a reference material for detecting genetically modified (GM) plants mixed into samples or for analyzing mixing ratios from tissue-cultured cell lines that are obtained by culturing tissues of either GM plants or non-GM plants. The present invention further relates to a method of detecting and analyzing ratios of GM plants mixed into samples using the reference material. With the reference material of the present invention, which is for detecting intermixing or for analyzing the mixing ratio of GM plants in samples by using tissue-cultured cell lines that are obtained by respectively culturing tissues of either GM plants or non-GM plants, countless individual lines having the same genetic traits can be obtained via the cell culturing, and hence the invention allows culturing capacity to be increased to large volumes in such a way as to produce large volumes of reference material of uniform quality free from quality variation between batches. In addition, reference materials manufactured by using grain powder, the present invention has advantages in that it allows either GM or non-GM reference materials with 100% purity to be obtained by verifying the purity of tissue-cultured cell lines, which enables a constant stable supply of reference materials with uniform compositions.
Inventors:
KIM HYONG HA (KR)
KIM WOO JEONG (KR)
SUH JUNG KEUN (KR)
KIM WOO JEONG (KR)
SUH JUNG KEUN (KR)
Application Number:
PCT/KR2010/009027
Publication Date:
June 23, 2011
Filing Date:
December 16, 2010
Export Citation:
Assignee:
KOREA RES INST OF STANDARDS (KR)
KIM HYONG HA (KR)
KIM WOO JEONG (KR)
SUH JUNG KEUN (KR)
KIM HYONG HA (KR)
KIM WOO JEONG (KR)
SUH JUNG KEUN (KR)
International Classes:
C12N5/04; C12N15/09; C12Q1/02; C12Q1/68
Foreign References:
| KR20030084184A | 2003-11-01 | |||
| KR100687765B1 | 2007-03-02 | |||
| KR100707830B1 | 2007-04-09 | |||
| KR100439168B1 | 2004-07-05 |
Other References:
ANKLAM ET AL., JOURNAL OF AOAC INTERNATIONAL., vol. 85, no. 3, 2002, pages 753
ANKLAM ET AL., JOURNAL OF AOAC INTERNATIONAL., vol. 85, no. 3, 2002, pages 809 - 815
See also references of EP 2514816A4
ANKLAM ET AL., JOURNAL OF AOAC INTERNATIONAL., vol. 85, no. 3, 2002, pages 809 - 815
See also references of EP 2514816A4
Attorney, Agent or Firm:
DANA PATENT LAW FIRM (KR)
특허법인다나 (KR)
특허법인다나 (KR)
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