Title:
METHOD FOR PCR CLEANUP AND OLIGONUCLEOTIDE REMOVAL
Document Type and Number:
WIPO Patent Application WO2004009851
Kind Code:
A3
Abstract:
A method is provided for purifying a desired polynucleotide product by removing unincorporated oligonucleotides from a polymerase or ligase reaction mixture.
Inventors:
GREENFIELD LAWRENCE (US)
BOST DOUGLAS A (US)
BOST DOUGLAS A (US)
Application Number:
PCT/US2003/023022
Publication Date:
August 25, 2005
Filing Date:
July 20, 2003
Export Citation:
Assignee:
APPLERA CORP (US)
GREENFIELD LAWRENCE (US)
BOST DOUGLAS A (US)
GREENFIELD LAWRENCE (US)
BOST DOUGLAS A (US)
International Classes:
C12Q1/68; (IPC1-7): C07H21/02; C07H21/04; C12Q1/68; C12P19/34
Other References:
BI W. AND STAMBROOK P.J.: "Detection of Known Mutation by Proof-Reading PCR", NUCLEIC ACID RESEARCH, vol. 26, no. 12, 1998, pages 3073 - 3075, XP002092520
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