Title:
METHOD FOR PREPARING HIGH-PURITY AND HIGH-STABILITY PROTEIN
Document Type and Number:
WIPO Patent Application WO/2024/087860
Kind Code:
A1
Abstract:
The present invention relates to a method for obtaining high-purity and high-stability recombinant human albumin by adding medium- and long-chain fatty acid ligands and removing a protein having charge heterogeneity by means of anion or/and cation chromatography. The present invention is characterized in that a mixed solution is added to a sample collection solution, the mixed solution is a mixed solution of a fatty acid mixture and poloxamer; the molar ratio of oleic acid to myristic acid to sodium palmitate to sodium stearate to recombinant human albumin is 0.3:0.3:0.3:0.5:1; and truncated albumin and mismatched or modified albumin in recombinant albumin can be effectively removed by means of sequential use of ion exchange chromatography, so that a high-purity and high-stability human albumin sample is obtained.
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Inventors:
XIANG WEI (CN)
YUE ZHILEI (CN)
DONG WENWEN (CN)
GUAN TINGCHEN (CN)
YUE ZHILEI (CN)
DONG WENWEN (CN)
GUAN TINGCHEN (CN)
Application Number:
PCT/CN2023/115220
Publication Date:
May 02, 2024
Filing Date:
August 28, 2023
Export Citation:
Assignee:
TONGHUA ANRATE BIOPHARMACEUTICAL CO LTD (CN)
International Classes:
C07K14/765; A61K38/00; A61K38/38; A61P7/00; C07K1/14; C07K1/18; C07K14/76; C12N15/14
Domestic Patent References:
| WO2022120547A1 | 2022-06-16 |
Foreign References:
| CN115677848A | 2023-02-03 | |||
| CN1611513A | 2005-05-04 | |||
| CN112516291A | 2021-03-19 | |||
| CN113831405A | 2021-12-24 |
Other References:
CHEN GUANG-MING: "Purification of Recombinant Human Serum Albumin from Fermentation Broth", CHINESE JOURNAL OF PHARMACEUTICAL BIOTECHNOLOGY, vol. 10, no. 1, 30 April 2003 (2003-04-30), pages 25 - 28, XP093164375
Attorney, Agent or Firm:
SHANGHAI ZHISHENG INTELLECTUAL PROPERTY OFFICE (CN)
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