Title:
METHOD FOR PREPARING RENAL PODOCYTES BY DISCONTINUOUS DIFFERENTIATION
Document Type and Number:
WIPO Patent Application WO/2023/155921
Kind Code:
A1
Abstract:
The present invention provides a method for preparing renal podocytes by discontinuous differentiation. Induced pluripotent stem cells are differentiated into the renal podocytes by using a discontinuous method. According to the method, pluripotent stem cells can be subjected to targeted induction of differentiation to obtain renal progenitor cells; renal progenitor cells with a high degree of differentiation are identified and cryopreserved, and then are thawed and subjected to targeted induction of differentiation into podocytes after large-scale preparation. The podocytes obtained by this method have high differentiation efficiency, high consistency, and a high survival rate.
Inventors:
WANG HENG (CN)
ZHENG LIXIN (CN)
QIU JIANG (CN)
CHEN JIAXIN (CN)
LIN KAIXIN (CN)
ZHENG LIXIN (CN)
QIU JIANG (CN)
CHEN JIAXIN (CN)
LIN KAIXIN (CN)
Application Number:
PCT/CN2023/077430
Publication Date:
August 24, 2023
Filing Date:
February 21, 2023
Export Citation:
Assignee:
GUANGZHOU ASIA KIDNEY REBUILDING MEDICAL TECH LTD (CN)
International Classes:
C12N5/071
Domestic Patent References:
| WO2016085765A1 | 2016-06-02 |
Foreign References:
| CN114457006A | 2022-05-10 | |||
| CN107208051A | 2017-09-26 | |||
| CN110662832A | 2020-01-07 | |||
| CN113943695A | 2022-01-18 | |||
| CN110662832A | 2020-01-07 |
Other References:
CIAMPI, O ET AL.: "Generation of functional podocytes from human induced pluripotent stem cells", STEM CELL RESEARCH, 3 June 2016 (2016-06-03), pages 130 - 139, XP029711969, DOI: 10.1016/j.scr.2016.06.001
LIU YILIN, WU ZHIPENG; QIU JIANG: "Research Progress and Challenges of Differentiation of Pluripotent Stem Cells into Kidney Organoids", CHINESE JOURNAL OF CELL AND STEM CELL(ELECTRONIC EDITION), vol. 11, no. 1, 28 February 2021 (2021-02-28), pages 57 - 62, XP093086565, ISSN: 2095-1221, DOI: 10.3877/cma.j.issn.2095-1221.2021.01.009
RAUCH CAROLINE, FEIFEL ELISABETH, KERN GEORG, MURPHY CORMAC, MEIER FLORIAN, PARSON WALTHER, BEILMANN MARIO, JENNINGS PAUL, GSTRAUN: "Differentiation of human iPSCs into functional podocytes", PLOS ONE, vol. 13, no. 9, 17 September 2018 (2018-09-17), pages e0203869, XP093086567, DOI: 10.1371/journal.pone.0203869
YOSHIMURA Y ET AL.: "Manipulation of Nephron-Patterning Signals Enables Selective Induction of Podocytes from Human Pluripotent Stem Cells [J", JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, vol. 30, no. 2, 2019, pages 2018070747
LIU YILIN, WU ZHIPENG; QIU JIANG: "Research Progress and Challenges of Differentiation of Pluripotent Stem Cells into Kidney Organoids", CHINESE JOURNAL OF CELL AND STEM CELL(ELECTRONIC EDITION), vol. 11, no. 1, 28 February 2021 (2021-02-28), pages 57 - 62, XP093086565, ISSN: 2095-1221, DOI: 10.3877/cma.j.issn.2095-1221.2021.01.009
RAUCH CAROLINE, FEIFEL ELISABETH, KERN GEORG, MURPHY CORMAC, MEIER FLORIAN, PARSON WALTHER, BEILMANN MARIO, JENNINGS PAUL, GSTRAUN: "Differentiation of human iPSCs into functional podocytes", PLOS ONE, vol. 13, no. 9, 17 September 2018 (2018-09-17), pages e0203869, XP093086567, DOI: 10.1371/journal.pone.0203869
YOSHIMURA Y ET AL.: "Manipulation of Nephron-Patterning Signals Enables Selective Induction of Podocytes from Human Pluripotent Stem Cells [J", JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, vol. 30, no. 2, 2019, pages 2018070747
Attorney, Agent or Firm:
JIAQUAN IP LAW (CN)
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