Title:
METHOD FOR PRODUCING B CELL MASS, AND METHOD FOR PRODUCING MONOCLONAL ANTIBODY EMPLOYING SAME
Document Type and Number:
WIPO Patent Application WO/2018/131698
Kind Code:
A1
Abstract:
The present invention addresses the problem of providing a method for highly efficiently producing a B cell mass containing B cells capable of recognizing a specific antigen. According to the present invention, a method for producing a B cell mass is provided, which comprises: step c of culturing a cell mass containing B cells together with a specific antigen in the absence of IL-21, in the absence of IL-4 and in the presence of a cytokine other than IL-21 or IL-4 while applying a stimulus through CD40 and a BAFF receptor and step d of culturing the cell mass containing the B cells while applying a stimulus through Fas to produce a B cell mass containing B cells capable of recognizing the specific antigen.
Inventors:
MOUTAI TATSUYA (JP)
NAKAISHI TOMOYUKI (JP)
KITA HIROSHI (JP)
KITANO MITSUAKI (JP)
KITAMURA DAISUKE (JP)
NAKAISHI TOMOYUKI (JP)
KITA HIROSHI (JP)
KITANO MITSUAKI (JP)
KITAMURA DAISUKE (JP)
Application Number:
PCT/JP2018/000757
Publication Date:
July 19, 2018
Filing Date:
January 15, 2018
Export Citation:
Assignee:
KANEKA CORP (JP)
UNIV TOKYO SCIENCE FOUND (JP)
UNIV TOKYO SCIENCE FOUND (JP)
International Classes:
C12N5/0781; C12P21/08
Domestic Patent References:
| WO2016002760A1 | 2016-01-07 | |||
| WO2016002760A1 | 2016-01-07 |
Foreign References:
| JP2011092142A | 2011-05-12 | |||
| JP2012029685A | 2012-02-16 | |||
| JPS5550132B2 | 1980-12-16 |
Other References:
KITAMURA, D.: "Generation of B- cell memory from the in-vitro-induced germinal center B cells", IMMUNOLOGY, vol. 137, no. 1, 2012, pages 332, XP009515568, DOI: 10.1111/imm.12002
NATURE, vol. 357, 1992, pages 80 - 82
EMBO J., vol. 11, 1992, pages 4313 - 4321
J EXP MED, vol. 189, 1999, pages 1747 - 1756
SCIENCE, vol. 285, 1999, pages 260 - 263
J BIO CHEM, vol. 274, 1999, pages 15978 - 15981
BLOOD, vol. 115, no. 9, 2010, pages 1718 - 26
JOURNAL OF IMMUNOLOGY, vol. 176, no. 10, 2006, pages 5890 - 7
CELL, vol. 75, 1993, pages 1169 - 1178
CANCER SCIENCE, vol. 105, 2014, pages 402 - 408
See also references of EP 3569700A4
NATURE, vol. 357, 1992, pages 80 - 82
EMBO J., vol. 11, 1992, pages 4313 - 4321
J EXP MED, vol. 189, 1999, pages 1747 - 1756
SCIENCE, vol. 285, 1999, pages 260 - 263
J BIO CHEM, vol. 274, 1999, pages 15978 - 15981
BLOOD, vol. 115, no. 9, 2010, pages 1718 - 26
JOURNAL OF IMMUNOLOGY, vol. 176, no. 10, 2006, pages 5890 - 7
CELL, vol. 75, 1993, pages 1169 - 1178
CANCER SCIENCE, vol. 105, 2014, pages 402 - 408
See also references of EP 3569700A4
Attorney, Agent or Firm:
SIKS & CO. (JP)
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