Title:
METHOD FOR PRODUCING OLIGONUCLEOTIDE
Document Type and Number:
WIPO Patent Application WO/2021/080021
Kind Code:
A1
Abstract:
The purpose of the present invention is to provide a method for producing an oligonucleotide and to provide an activating agent for producing the oligonucleotide. The aforementioned problem is solved by a method for producing an oligonucleotide, wherein said method comprises a step in which a nucleoside phosphoramidite having a protective group linked to a hydroxyl group at position 5' and a nucleoside having an unprotected hydroxyl group at the 3' position and the 5' position are reacted in the presence of an activating agent represented by formula (I).
Inventors:
IWAMOTO MASAFUMI (JP)
KATAOKA MASANORI (JP)
IIDA TOMOYOSHI (JP)
KATAOKA MASANORI (JP)
IIDA TOMOYOSHI (JP)
Application Number:
PCT/JP2020/040096
Publication Date:
April 29, 2021
Filing Date:
October 26, 2020
Export Citation:
Assignee:
NITTO DENKO CORP (JP)
NATIAS INC (JP)
NATIAS INC (JP)
International Classes:
C07H21/04
Domestic Patent References:
| WO2012024776A1 | 2012-03-01 | |||
| WO2018152453A1 | 2018-08-23 | |||
| WO2005082923A1 | 2005-09-09 | |||
| WO2019212061A1 | 2019-11-07 |
Foreign References:
| JP2003012690A | 2003-01-15 | |||
| JP2007531794A | 2007-11-08 | |||
| JP2001192395A | 2001-07-17 | |||
| JPH1180185A | 1999-03-26 | |||
| JP2001247596A | 2001-09-11 | |||
| JP2000344792A | 2000-12-12 | |||
| JP2008530092A | 2008-08-07 |
Other References:
QIBIN ZHANG AND YINSHENG WANG: "Independent Generation of the 5-Hydroxy-5, 6-dihydrothymidin-6-yl Radical and Its Reactivity in Dinucleoside Monophosphates", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 126, no. 41, 2004, pages 13287 - 13297, XP055820544
HAYAKAWA, Y. ET AL.: "Acid/Azole Complexes as Highly Effective Promoters in the Synthesis of DNA and RNA Oligomers via the Phosphoramidite Method", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 123, no. 34, 2001, pages 8165 - 8176, XP001188947, DOI: 10.1021/ja010078b
SCHELL P; ENGELS J W: "Rp-Diastereoselective Synthesis of Dinucleoside Methylphosphonates by the Phosphoramidite Approach", TETRAHEDRON LETTERS, vol. 39, no. 47, 1998, pages 8629 - 8632, XP004140599, DOI: 10.1016/S0040-4039(98)01972-8
HAYAKAWA, Y. ET AL.: "Acid/Azole Complexes as Highly Effective Promoters in the Synthesis of DNA and RNA Oligomers via the Phosphoramidite Method", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 123, no. 34, 2001, pages 8165 - 8176, XP001188947, DOI: 10.1021/ja010078b
SCHELL P; ENGELS J W: "Rp-Diastereoselective Synthesis of Dinucleoside Methylphosphonates by the Phosphoramidite Approach", TETRAHEDRON LETTERS, vol. 39, no. 47, 1998, pages 8629 - 8632, XP004140599, DOI: 10.1016/S0040-4039(98)01972-8
Attorney, Agent or Firm:
KUZUWA, Kiyoshi (JP)
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