Title:
METHOD FOR PRODUCING TEMPERATURE-SENSITIVE MALE STERILE PLANT
Document Type and Number:
WIPO Patent Application WO/2021/193865
Kind Code:
A1
Abstract:
The entire genomes of "PL12" and the original variety thereof were sequenced and compared in order to identify the responsible gene involved in a temperature-sensitive male sterility trait in "PL12" and to provide a method for producing a conditional male sterile plant targeting the gene. As a result, it was found that there was approximately 150 kb of deletion in the seventh chromosome of "PL12". Regarding this region, six types of partial deletion lines were produced by genome editing, and the range in which the responsible mutation was present was successfully narrowed to approximately 10 kb with the presence or absence of a temperature-sensitive male sterility trait in each of the lines as an indicator. Further, as a result of producing a line in which the functions of two genes located in this region were disrupted by genome editing, it was possible to identify the responsible gene involved in the temperature-sensitive male sterility trait. It was also confirmed that the function of the gene was inhibited, and the trait was able to be imparted to Arabidopsis thaliana and tomato as well as rice.
Inventors:
KAWAGISHI MAKIKO (JP)
SASAKI KENTARO (JP)
NUNOME TSUKASA (JP)
SASAKI KENTARO (JP)
NUNOME TSUKASA (JP)
Application Number:
PCT/JP2021/012709
Publication Date:
September 30, 2021
Filing Date:
March 25, 2021
Export Citation:
Assignee:
NAT AGRICULTURE & FOOD RES ORG (JP)
International Classes:
A01H1/00; A01H1/02; A01H5/10; C12N15/29
Domestic Patent References:
| WO2013176772A1 | 2013-11-28 |
Foreign References:
| CN101513169A | 2009-08-26 | |||
| CN108341860A | 2018-07-31 | |||
| JP2018532393A | 2018-11-08 | |||
| JPH02207722A | 1990-08-17 | |||
| JP2017535294A | 2017-11-30 | |||
| JP2018535672A | 2018-12-06 | |||
| US6265196B1 | 2001-07-24 | |||
| US8524500B2 | 2013-09-03 | |||
| US7888121B2 | 2011-02-15 | |||
| EP1720995A2 | 2006-11-15 | |||
| US8470973B2 | 2013-06-25 | |||
| US8586363B2 | 2013-11-19 | |||
| US8697359B1 | 2014-04-15 | |||
| US5416011A | 1995-05-16 |
Other References:
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J.H.TANG ET AL.: "Genetic analyses and mapping of a new thermo-sensitive genic male sterile gene in maize", THEORETICAL AND APPLIED GENETICS, vol. 113, 2006, pages 11 - 15, XP002463860
ANONYMOUS: "Rice Genome Annnotation Project Funded by the NSF", PLANTBIOLOGY, 16 April 2021 (2021-04-16), pages 1 - 2, XP055862429, Retrieved from the Internet
YAMAGUCHI ET AL., BREEDING SCIENCE, vol. 47, 1997, pages 371 - 373
CHUEASIRI ET AL., PLOS ONE, vol. 9, no. 9, 2014, pages e106386
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SAIKI, R. K. ET AL., SCIENCE, vol. 230, 1985, pages 1350 - 1354
SAIKI, R. K. ET AL., SCIENCE, vol. 239, 1988, pages 487 - 491
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 2268
A. M. CASAS ET AL., PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 11212 - 11216
ALTSCHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
NAKAMURA ET AL., PLANT CELL PHYSIOL, vol. 53, 2012, pages 1171 - 1179
ZETSCHE B. ET AL., CELL, vol. 163, no. 3, 2015, pages 759 - 71
K. NISHIDA ET AL.: "Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems", SCIENCE, DOI:10.1126/SCIENCE.AAF8729, 2016
HAYASHI ET AL., CYCLOTRONS AND THEIR APPLICATIONS, 2007, pages 237 - 239
KAZAMA ET AL., PLANT BIOTECHNOLOGY, vol. 25, 2008, pages 113 - 117
ZWARCHANDLER, PLANTA, vol. 197, 1995, pages 39 - 48
KUMAR ET AL., TRENDS PLANT SCI., vol. 6, 2001, pages 127 - 134
TAMARA ET AL., TRENDS IN PLANT SCIENCE, vol. 4, 1999, pages 90 - 96
SLADE ET AL., TRANSGENIC RES., vol. 14, 2005, pages 109 - 115
COMAI ET AL., PLANT J., vol. 37, 2004, pages 778 - 786
DATTA, S. K.: "Gene Transfer To Plants", 1995, pages: 66 - 74
TOKI ET AL., PLANT PHYSIOL., vol. 100, 1992, pages 1503 - 1507
CHRISTOU ET AL., BIO/TECHNOLOGY, vol. 9, 1991, pages 957 - 962
HIEI ET AL., PLANT J., vol. 6, 1994, pages 271 - 282
CLOUGH SJBENT AF, PLANT J, vol. 16, 1998, pages 735 - 743
AKAMA ET AL., PLANT CELL REPORTS, vol. 12, 1992, pages 7 - 11
TINGAY S. ET AL., PLANT J., vol. 11, 1997, pages 1369 - 1376
MURRAY F ET AL., PLANT CELL REPORT, vol. 22, 2004, pages 397 - 402
TRAVALLA S ET AL., PLANT CELL REPORT, vol. 23, 2005, pages 780 - 789
J. A. ABLE ET AL., VITRO CELL. DEV. BIOL., vol. 37, 2001, pages 341 - 348
V. GIRIJASHANKAR ET AL., PLANT CELL REP, vol. 24, no. 9, 2005, pages 513 - 522
J. M. JEOUNG ET AL., HEREDITAS, vol. 137, 2002, pages 20 - 28
ZUO-YU ZHAO ET AL., PLANT MOLECULAR BIOLOGY, vol. 44, 2000, pages 789 - 798
S. GUREL ET AL., PLANT CELL REP, vol. 28, no. 3, 2009, pages 429 - 444
ZY ZHAO, METHODS MOL BIOL, vol. 343, 2006, pages 233 - 244
AK SHRAWATH LORZ, PLANT BIOTECHNOL J, vol. 4, no. 6, 2006, pages 575 - 603
D SYAMALAP DEVI, INDIAN J EXP BIOL, vol. 41, no. 12, 2003, pages 1482 - 1486
Z GAO ET AL., PLANT BIOTECHNOL J, vol. 3, no. 6, 2005, pages 591 - 599
SHILLITO, BIO/TECHNOLOGY, vol. 7, 1989, pages 581
GORDEN-KAMM ET AL., PLANT CELL, vol. 2, 1990, pages 603
MATSUKURA ET AL., J. EXP. BOT., vol. 44, 1993, pages 1837 - 1845
VISSER ET AL., THEOR. APPL. GENET, vol. 78, 1989, pages 594
YUTAKA TABEI: "Protocols for Plant Transformation", 20 September 2012, KAGAKU-DOJIN PUBLISHING
UETA ET AL.: "Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9", SCI REP, vol. 7, 2017, pages 507, XP055641387, DOI: 10.1038/s41598-017-00501-4
SUN ET AL.: "A Highly Efficient Transformation Protocol for MicroTom, a Model Cultivar for Tomato Functional Genomics", PLANT CELL PHYSIOL., vol. 47, 2006, pages 426 - 431
See also references of EP 4129050A4
J.H.TANG ET AL.: "Genetic analyses and mapping of a new thermo-sensitive genic male sterile gene in maize", THEORETICAL AND APPLIED GENETICS, vol. 113, 2006, pages 11 - 15, XP002463860
ANONYMOUS: "Rice Genome Annnotation Project Funded by the NSF", PLANTBIOLOGY, 16 April 2021 (2021-04-16), pages 1 - 2, XP055862429, Retrieved from the Internet
YAMAGUCHI ET AL., BREEDING SCIENCE, vol. 47, 1997, pages 371 - 373
CHUEASIRI ET AL., PLOS ONE, vol. 9, no. 9, 2014, pages e106386
PITNJAM ET AL., PLANTA, vol. 228, 2008, pages 813 - 822
SOUTHERN, E. M., J. MOL. BIOL., vol. 98, 1975, pages 503
SAIKI, R. K. ET AL., SCIENCE, vol. 230, 1985, pages 1350 - 1354
SAIKI, R. K. ET AL., SCIENCE, vol. 239, 1988, pages 487 - 491
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 410
KARLINALTSCHUL, PROC. NATL. ACAD. SCI. USA, vol. 87, 1990, pages 2264 - 2268
A. M. CASAS ET AL., PROC. NATL. ACAD. SCI. USA, vol. 90, 1993, pages 11212 - 11216
ALTSCHUL ET AL., NUCLEIC ACIDS RES., vol. 25, 1997, pages 3389 - 3402
NAKAMURA ET AL., PLANT CELL PHYSIOL, vol. 53, 2012, pages 1171 - 1179
ZETSCHE B. ET AL., CELL, vol. 163, no. 3, 2015, pages 759 - 71
K. NISHIDA ET AL.: "Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems", SCIENCE, DOI:10.1126/SCIENCE.AAF8729, 2016
HAYASHI ET AL., CYCLOTRONS AND THEIR APPLICATIONS, 2007, pages 237 - 239
KAZAMA ET AL., PLANT BIOTECHNOLOGY, vol. 25, 2008, pages 113 - 117
ZWARCHANDLER, PLANTA, vol. 197, 1995, pages 39 - 48
KUMAR ET AL., TRENDS PLANT SCI., vol. 6, 2001, pages 127 - 134
TAMARA ET AL., TRENDS IN PLANT SCIENCE, vol. 4, 1999, pages 90 - 96
SLADE ET AL., TRANSGENIC RES., vol. 14, 2005, pages 109 - 115
COMAI ET AL., PLANT J., vol. 37, 2004, pages 778 - 786
DATTA, S. K.: "Gene Transfer To Plants", 1995, pages: 66 - 74
TOKI ET AL., PLANT PHYSIOL., vol. 100, 1992, pages 1503 - 1507
CHRISTOU ET AL., BIO/TECHNOLOGY, vol. 9, 1991, pages 957 - 962
HIEI ET AL., PLANT J., vol. 6, 1994, pages 271 - 282
CLOUGH SJBENT AF, PLANT J, vol. 16, 1998, pages 735 - 743
AKAMA ET AL., PLANT CELL REPORTS, vol. 12, 1992, pages 7 - 11
TINGAY S. ET AL., PLANT J., vol. 11, 1997, pages 1369 - 1376
MURRAY F ET AL., PLANT CELL REPORT, vol. 22, 2004, pages 397 - 402
TRAVALLA S ET AL., PLANT CELL REPORT, vol. 23, 2005, pages 780 - 789
J. A. ABLE ET AL., VITRO CELL. DEV. BIOL., vol. 37, 2001, pages 341 - 348
V. GIRIJASHANKAR ET AL., PLANT CELL REP, vol. 24, no. 9, 2005, pages 513 - 522
J. M. JEOUNG ET AL., HEREDITAS, vol. 137, 2002, pages 20 - 28
ZUO-YU ZHAO ET AL., PLANT MOLECULAR BIOLOGY, vol. 44, 2000, pages 789 - 798
S. GUREL ET AL., PLANT CELL REP, vol. 28, no. 3, 2009, pages 429 - 444
ZY ZHAO, METHODS MOL BIOL, vol. 343, 2006, pages 233 - 244
AK SHRAWATH LORZ, PLANT BIOTECHNOL J, vol. 4, no. 6, 2006, pages 575 - 603
D SYAMALAP DEVI, INDIAN J EXP BIOL, vol. 41, no. 12, 2003, pages 1482 - 1486
Z GAO ET AL., PLANT BIOTECHNOL J, vol. 3, no. 6, 2005, pages 591 - 599
SHILLITO, BIO/TECHNOLOGY, vol. 7, 1989, pages 581
GORDEN-KAMM ET AL., PLANT CELL, vol. 2, 1990, pages 603
MATSUKURA ET AL., J. EXP. BOT., vol. 44, 1993, pages 1837 - 1845
VISSER ET AL., THEOR. APPL. GENET, vol. 78, 1989, pages 594
YUTAKA TABEI: "Protocols for Plant Transformation", 20 September 2012, KAGAKU-DOJIN PUBLISHING
UETA ET AL.: "Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9", SCI REP, vol. 7, 2017, pages 507, XP055641387, DOI: 10.1038/s41598-017-00501-4
SUN ET AL.: "A Highly Efficient Transformation Protocol for MicroTom, a Model Cultivar for Tomato Functional Genomics", PLANT CELL PHYSIOL., vol. 47, 2006, pages 426 - 431
See also references of EP 4129050A4
Attorney, Agent or Firm:
CENTCREST IP ATTORNEYS (JP)
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