Title:
METHOD FOR PRODUCING TRANSFORMED CELLS OF CORN OR CORN PLANT, NUCLEIC ACID CONSTRUCT, AND METHOD FOR INTRODUCING NUCLEIC ACID TO CELLS OR CORN PLANT
Document Type and Number:
WIPO Patent Application WO/2022/145427
Kind Code:
A1
Abstract:
The present invention pertains to a method for producing transformed cells of corn or corn plant The present invention also pertains to a method for introducing a nucleic acid to corn cells or corn plant. The present invention further pertains to a nucleic acid construct. This method for producing transformed corn cells or a corn plant includes: overexpressing in corn 1) a nucleic acid that codes for the amino acid sequence represented by SEQ ID NO: 2, or a nucleic acid that codes for a polypeptide which includes an amino acid sequence having at least 95% identity with the amino acid sequence represented by SEQ ID NO: 2 and which has a corn cell division-promoting function; or 2) the nucleic acid that codes for the amino acid sequence represented by SEQ ID NO: 15, or a nucleic acid that codes for a polypeptide which includes an amino acid sequence having at least 95% identity with the amino acid sequence represented by SEQ ID NO: 15 and which has a corn cell division-promoting function.
Inventors:
ISHIDA YUJI (JP)
ICHIKAWA MASAKO (JP)
ICHIKAWA MASAKO (JP)
Application Number:
PCT/JP2021/048621
Publication Date:
July 07, 2022
Filing Date:
December 27, 2021
Export Citation:
Assignee:
KANEKA CORP (JP)
International Classes:
A01H5/00; A01H1/00; A01H4/00; A01H6/46; C12N5/10; C12N15/29; C12N15/82
Domestic Patent References:
| WO2018224001A1 | 2018-12-13 | |||
| WO2021153163A1 | 2021-08-05 | |||
| WO2021030242A1 | 2021-02-18 | |||
| WO2018224001A1 | 2018-12-13 | |||
| WO2007148819A1 | 2007-12-27 | |||
| WO2014157541A1 | 2014-10-02 |
Foreign References:
| US20110258741A1 | 2011-10-20 | |||
| JP2018537080A | 2018-12-20 | |||
| US20200270622A1 | 2020-08-27 | |||
| US8383891B2 | 2013-02-26 |
Other References:
ISHIDA YUJI; HIEI YUKOH; KOMARI TOSHIHIKO: "Agrobacterium-mediated transformation of maize", NATURE PROTOCOLS, NATURE PUBLISHING GROUP, GB, vol. 2, no. 7, 1 July 2007 (2007-07-01), GB , pages 1614 - 1621, XP009148413, ISSN: 1750-2799, DOI: 10.1038/nprot.2007.241
DATABASE NUCLEOTIDE 31 August 2020 (2020-08-31), ANONYMOUS : "PREDICTED: Zea mays WUSCHEL-related homeobox 9-like (LOC103650992), transcript variant X1, mRNA", XP055948720, retrieved from NCBI Database accession no. XM_020550030
DATABASE PROTEIN 24 October 2013 (2013-10-24), ANONYMOUS : "TPA: putative homeobox DNA-binding domain superfamily protein [Zea mays]", XP055948722, retrieved from NCBI Database accession no. DAA56787
KYO ET AL.: "Coexpression of WUSCHEL related homeobox (WOX) 2 with WOX8 or WOX9 promotes regeneration from leaf segments and free cells in Nicotiana tabacum L", PLANT BIOTECHNOLOGY, vol. 35, 2018, pages 23 - 30
ISHIDA ET AL.: "Agrobacterium-mediated transformation of maize", NAT. PROTOC., vol. 2, 2007, pages 1614 - 1621, XP009148413, DOI: 10.1038/nprot.2007.241
CHIU ET AL.: "Engineered GFP as a vital reporter in plants", CURRENT BIOLOGY, vol. 6, no. 3, 1996, pages 325 - 330, XP000571865, DOI: 10.1016/S0960-9822(02)00483-9
TODD J. JONES: "Tissue Culture and Transformation: The First 20 Years", MOLECULAR GENETIC APPROACHES TO MAIZE IMPROVEMENT, vol. 7, 2009, pages 27
TODA ET AL.: "An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice", NATURE PLANTS, vol. 5, 2019, pages 363 - 368, Retrieved from the Internet
OHTA ET AL.: "Construction and Expression in Tobacco of a β-Glucuronidase (GUS) Reporter Gene Containing an Intron Within the Coding Sequence", PLANT CELL PHYSIOL., vol. 31, no. 6, 1990, pages 805 - 813
KOISO ET AL.: "Development of gene expression system in egg cells and zygotes isolated from rice and maize", PLANT DIRECT, vol. 1, no. 3, 2017, pages e00010, Retrieved from the Internet
LOWE ET AL.: "Rapid genotype ''independent'' Zea mays L. (maize) transformation via direct somatic embryogenesis", IN VITRO CELLULAR DEVELOPMENTAL BIOLOGY - PLANT, vol. 54, 2018, pages 240 - 252, XP036504790, Retrieved from the Internet DOI: 10.1007/s11627-018-9905-2
NARDMANN ET AL.: "WOX Gene Phylogeny in Poaceae: A Comparative Approach Addressing Leaf and Embryo Development", MOLECULAR BIOLOGY AND EVOLUTION, vol. 24, no. 11, 2007, pages 2474 - 84, Retrieved from the Internet
ARMSTRONGGREEN: "Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline", PLANTA, vol. 164, 1985, pages 207 - 214, XP002914177, DOI: 10.1007/BF00396083
RHODES ET AL.: "Plant Regeneration from Protoplasts Isolated from Embryogenic Maize Cell Cultures", NATURE BIOTECHNOLOGY, vol. 6, 1988, pages 56 - 60, XP002117775, DOI: 10.1038/nbt0188-56
FROMM ET AL.: "Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants", BIOTECHNOLOGY, vol. 8, 1990, pages 833 - 839, XP002910122, DOI: 10.1038/nbt0990-833
GORDON-KAMM WJ ET AL.: "Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants", PLANT CELL, vol. 2, 1990, pages 603 - 618, XP002059217, DOI: 10.1105/tpc.2.7.603
FRAME ET AL.: "Production of fertile transgenic maize plants by silicon carbide whisker-mediated transformation", PLANT JOURNAL, vol. 6, no. 6, 1994, pages 941 - 948, XP002909291, DOI: 10.1046/j.1365-313X.1994.6060941.x
ISHIDA ET AL.: "High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens", NATURE BIOTECHNOLOGY, vol. 14, 1996, pages 745 - 750, XP002046364, DOI: 10.1038/nbt0696-745
LOWE ET AL.: "Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation", THE PLANT CELL, vol. 28, 2016, pages 1998 - 2015, XP055318109, DOI: 10.1105/tpc.16.00124
PRIOLI ET AL.: "Plant Regeneration and Recovery of Fertile Plants from Protoplasts of Maize (Zea Mays L.", NATURE BIOTECHNOLOGY, vol. 7, 1989, pages 589 - 594
SHILLITO ET AL.: "Regeneration of Fertile Plants from Protoplasts of Elite Inbread Maize", NATURE BIOTECHNOLOGY, vol. 7, 1989, pages 581 - 587
YAU, Y.Y.STEWART, C.N., JR.: "Less is more: strategies to remove marker genes from transgenic plants", BMC BIOTECHNOL, vol. 13, 2013, pages 36, XP021153185, DOI: 10.1186/1472-6750-13-36
HIEI ET AL.: "Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed", NAT. PROTOC., vol. 3, 2008, pages 824 - 834
"Genbank", Database accession no. XM_020550030
ALTSCHUL ET AL., NUCL. ACIDS. RES., vol. 25, 1997, pages 3389 - 3402
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", vol. 3, 2001, COLD SPRING HARBOR LABORATORY PRESS
KOMARI ET AL., PLANT J, vol. 10, 1996, pages 165 - 174
KOMORI ET AL., PLANT J, vol. 37, 2004, pages 315 - 325
TODA ET AL., SUPPLEMENTARY METHODS, 2019
ISHIDA ET AL., NATURE PROTOCOLS, vol. 2, no. 7, 2007, pages 1614 - 1621
DATABASE NUCLEOTIDE 31 August 2020 (2020-08-31), ANONYMOUS : "PREDICTED: Zea mays WUSCHEL-related homeobox 9-like (LOC103650992), transcript variant X1, mRNA", XP055948720, retrieved from NCBI Database accession no. XM_020550030
DATABASE PROTEIN 24 October 2013 (2013-10-24), ANONYMOUS : "TPA: putative homeobox DNA-binding domain superfamily protein [Zea mays]", XP055948722, retrieved from NCBI Database accession no. DAA56787
KYO ET AL.: "Coexpression of WUSCHEL related homeobox (WOX) 2 with WOX8 or WOX9 promotes regeneration from leaf segments and free cells in Nicotiana tabacum L", PLANT BIOTECHNOLOGY, vol. 35, 2018, pages 23 - 30
ISHIDA ET AL.: "Agrobacterium-mediated transformation of maize", NAT. PROTOC., vol. 2, 2007, pages 1614 - 1621, XP009148413, DOI: 10.1038/nprot.2007.241
CHIU ET AL.: "Engineered GFP as a vital reporter in plants", CURRENT BIOLOGY, vol. 6, no. 3, 1996, pages 325 - 330, XP000571865, DOI: 10.1016/S0960-9822(02)00483-9
TODD J. JONES: "Tissue Culture and Transformation: The First 20 Years", MOLECULAR GENETIC APPROACHES TO MAIZE IMPROVEMENT, vol. 7, 2009, pages 27
TODA ET AL.: "An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice", NATURE PLANTS, vol. 5, 2019, pages 363 - 368, Retrieved from the Internet
OHTA ET AL.: "Construction and Expression in Tobacco of a β-Glucuronidase (GUS) Reporter Gene Containing an Intron Within the Coding Sequence", PLANT CELL PHYSIOL., vol. 31, no. 6, 1990, pages 805 - 813
KOISO ET AL.: "Development of gene expression system in egg cells and zygotes isolated from rice and maize", PLANT DIRECT, vol. 1, no. 3, 2017, pages e00010, Retrieved from the Internet
LOWE ET AL.: "Rapid genotype ''independent'' Zea mays L. (maize) transformation via direct somatic embryogenesis", IN VITRO CELLULAR DEVELOPMENTAL BIOLOGY - PLANT, vol. 54, 2018, pages 240 - 252, XP036504790, Retrieved from the Internet
NARDMANN ET AL.: "WOX Gene Phylogeny in Poaceae: A Comparative Approach Addressing Leaf and Embryo Development", MOLECULAR BIOLOGY AND EVOLUTION, vol. 24, no. 11, 2007, pages 2474 - 84, Retrieved from the Internet
ARMSTRONGGREEN: "Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline", PLANTA, vol. 164, 1985, pages 207 - 214, XP002914177, DOI: 10.1007/BF00396083
RHODES ET AL.: "Plant Regeneration from Protoplasts Isolated from Embryogenic Maize Cell Cultures", NATURE BIOTECHNOLOGY, vol. 6, 1988, pages 56 - 60, XP002117775, DOI: 10.1038/nbt0188-56
FROMM ET AL.: "Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants", BIOTECHNOLOGY, vol. 8, 1990, pages 833 - 839, XP002910122, DOI: 10.1038/nbt0990-833
GORDON-KAMM WJ ET AL.: "Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants", PLANT CELL, vol. 2, 1990, pages 603 - 618, XP002059217, DOI: 10.1105/tpc.2.7.603
FRAME ET AL.: "Production of fertile transgenic maize plants by silicon carbide whisker-mediated transformation", PLANT JOURNAL, vol. 6, no. 6, 1994, pages 941 - 948, XP002909291, DOI: 10.1046/j.1365-313X.1994.6060941.x
ISHIDA ET AL.: "High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens", NATURE BIOTECHNOLOGY, vol. 14, 1996, pages 745 - 750, XP002046364, DOI: 10.1038/nbt0696-745
LOWE ET AL.: "Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation", THE PLANT CELL, vol. 28, 2016, pages 1998 - 2015, XP055318109, DOI: 10.1105/tpc.16.00124
PRIOLI ET AL.: "Plant Regeneration and Recovery of Fertile Plants from Protoplasts of Maize (Zea Mays L.", NATURE BIOTECHNOLOGY, vol. 7, 1989, pages 589 - 594
SHILLITO ET AL.: "Regeneration of Fertile Plants from Protoplasts of Elite Inbread Maize", NATURE BIOTECHNOLOGY, vol. 7, 1989, pages 581 - 587
YAU, Y.Y.STEWART, C.N., JR.: "Less is more: strategies to remove marker genes from transgenic plants", BMC BIOTECHNOL, vol. 13, 2013, pages 36, XP021153185, DOI: 10.1186/1472-6750-13-36
HIEI ET AL.: "Agrobacterium-mediated transformation of rice using immature embryos or calli induced from mature seed", NAT. PROTOC., vol. 3, 2008, pages 824 - 834
"Genbank", Database accession no. XM_020550030
ALTSCHUL ET AL., NUCL. ACIDS. RES., vol. 25, 1997, pages 3389 - 3402
ALTSCHUL ET AL., J. MOL. BIOL., vol. 215, 1990, pages 403 - 10
SAMBROOK ET AL.: "Molecular Cloning: A Laboratory Manual", vol. 3, 2001, COLD SPRING HARBOR LABORATORY PRESS
KOMARI ET AL., PLANT J, vol. 10, 1996, pages 165 - 174
KOMORI ET AL., PLANT J, vol. 37, 2004, pages 315 - 325
TODA ET AL., SUPPLEMENTARY METHODS, 2019
ISHIDA ET AL., NATURE PROTOCOLS, vol. 2, no. 7, 2007, pages 1614 - 1621
Attorney, Agent or Firm:
YAMAMOTO, Osamu et al. (JP)
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