Title:
METHOD FOR ULTRA-RAPIDLY SELECTING SIGNAL PEPTIDE TO WHICH INDIVIDUAL BARCODE SYSTEM FOR INCREASING PROTEIN PRODUCTIVITY IS INTRODUCED
Document Type and Number:
WIPO Patent Application WO/2021/045541
Kind Code:
A1
Abstract:
The present invention relates to a composition for selecting, from among various signal peptides, the optimum signal peptide for effectively secreting a target protein to the outside of cells. In addition, the present invention relates to a method for selecting a particular signal peptide, which expresses a target protein in a host cell so as to effectively secret the target protein to the outside of the host cell. When the composition and/or the selection method of the present invention is used, a signal peptide that is optimal for a target protein can be ultra-rapidly selected using a barcode sequence made for each signal peptide, so that the production yield of a recombinant protein can be maximized.
Inventors:
CHOI SO YOUNG (KR)
PARK JI HOON (KR)
JIN JONGHWA (KR)
KIM SUNG SUB (KR)
JANG MYUNG-HEE (KR)
PARK JI HOON (KR)
JIN JONGHWA (KR)
KIM SUNG SUB (KR)
JANG MYUNG-HEE (KR)
Application Number:
PCT/KR2020/011885
Publication Date:
March 11, 2021
Filing Date:
September 03, 2020
Export Citation:
Assignee:
OSONG MEDICAL INNOVATION FOUND (KR)
IAC IN NAT UNIV CHUNGNAM (KR)
IAC IN NAT UNIV CHUNGNAM (KR)
International Classes:
C12N15/10; C40B40/08; C40B50/06; G01N30/72; G01N33/68
Domestic Patent References:
| WO2015133074A1 | 2015-09-11 |
Foreign References:
| KR101875355B1 | 2018-07-05 | |||
| US20180080021A1 | 2018-03-22 | |||
| US20170137806A1 | 2017-05-18 |
Other References:
LEE JUHYEOP: "HT System for Selection and Application of Optimal Signal Peptide for Development of Secretion System for Enhancing Protein Expression", OSONG MEDICAL INNOVATION FOUNDATION. TECHNICAL MANUAL., 4 April 2019 (2019-04-04), XP055787678, Retrieved from the Internet
ACTA BIOCHIM BIOPHYS SIN (SHANGHAI, vol. 43, no. 2, 2011, pages 96 - 102
BIOCHEM BIOPHYS RES COMMUN., vol. 391, no. 1, 1 January 2010 (2010-01-01), pages 931 - 5
MANIATIS ET AL.: "Molecular Cloning, A Laboratory Manual", 1988, COLD SPRING HARBOR PRESS
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1994, JOHN, WILEY & SONS, INC
ACTA BIOCHIM BIOPHYS SIN (SHANGHAI, vol. 43, no. 2, 2011, pages 96 - 102
BIOCHEM BIOPHYS RES COMMUN., vol. 391, no. 1, 1 January 2010 (2010-01-01), pages 931 - 5
MANIATIS ET AL.: "Molecular Cloning, A Laboratory Manual", 1988, COLD SPRING HARBOR PRESS
AUSUBEL ET AL.: "Current Protocols in Molecular Biology", 1994, JOHN, WILEY & SONS, INC
Attorney, Agent or Firm:
CHUNG HYUN PATENT & LAW FIRM (KR)
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