Title:
METHODS AND APPARATUSES FOR SCALING UP CELL-FREE SYNTHESIS REACTIONS
Document Type and Number:
WIPO Patent Application WO/2023/094884
Kind Code:
A8
Abstract:
A cartridge for cell-free enzymatic production and methods of use there of. The cartridge comprising a flat reaction chamber with hydrophobic gas-permeable membrane(s) on one or both major surfaces, an inlet, and wherein the height or width or both of the reaction chamber is 5 times the value of the thickness between the major surfaces. The cartridge further comprising porous fill material in the reaction chamber, a hydrophobic frit on the outlet, an enclosure over the cartridge for controlling humidity, the inlet comprising a slot with a key-hole opening for positioning a pipette tip and wherein extraction can occur through the membrane acting as a pressure-responsive valve.
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Inventors:
KLENOV ALEXANDER (CA)
TINAFAR HAMED (CA)
TINAFAR HAMED (CA)
Application Number:
PCT/IB2022/000680
Publication Date:
June 22, 2023
Filing Date:
November 23, 2022
Export Citation:
Assignee:
LIBERUM BIOTECH INC (CA)
International Classes:
C12M1/40; C12M1/12; C12P1/00
Attorney, Agent or Firm:
SALISBURY, Clare et al. (CA)
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