Title:
METHODS FOR MODULATING THE NICOTINE LEVEL IN NICOTIANA TABACUM
Document Type and Number:
WIPO Patent Application WO/2021/205000
Kind Code:
A3
Abstract:
The present invention provides a method for modulating (e.g. decreasing) the nicotine content of a plant (e.g. a tobacco plant) or part thereof, or tobacco plant cell, the method comprising modifying said plant or cell providing at least one mutation in a Nic3 locus. The present invention provides a method for modulating (e.g. decreasing) the nicotine content of a plant (e.g. a tobacco plant) or part thereof, or tobacco plant cell, the method comprising modifying said plant or cell to modulate the expression or activity of at least one Nic3 gene. The present invention also provides for the use of the Nic3 locus for modulating the alkaloid content of a plant, as well as tobacco cells, plants, plant propagation materials, harvested leaves, processed tobaccos, or delivery systems obtainable in accordance with the invention.
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Inventors:
LI XINGPENG (US)
LAWSON DARLENE MADELINE (US)
HUMPHRY MATTHEW EDWARD (GB)
KHALED SARA BEN (GB)
LAWSON DARLENE MADELINE (US)
HUMPHRY MATTHEW EDWARD (GB)
KHALED SARA BEN (GB)
Application Number:
PCT/EP2021/059302
Publication Date:
December 23, 2021
Filing Date:
April 09, 2021
Export Citation:
Assignee:
REYNOLDS TOBACCO CO R (US)
BRITISH AMERICAN TOBACCO INVESTMENTS LTD (GB)
BRITISH AMERICAN TOBACCO INVESTMENTS LTD (GB)
International Classes:
A01H1/00; A01H5/12; A01H6/82; A24B13/00; A24D1/00; C07K14/415; C12N15/82; C12Q1/6876
Domestic Patent References:
| WO2016210303A1 | 2016-12-29 |
Foreign References:
| US20140246036A1 | 2014-09-04 |
Other References:
WANG BINGWU ET AL: "Genetic Factors for Enhancement of Nicotine Levels in Cultivated Tobacco", SCIENTIFIC REPORTS, vol. 5, no. 1, 2 December 2015 (2015-12-02), pages 1 - 10, XP055821817, Retrieved from the Internet DOI: 10.1038/srep17360
TODD ANDREA T. ET AL: "A functional genomics screen identifies diverse transcription factors that regulate alkaloid biosynthesis in Nicotiana benthamiana : TFs regulating nicotine biosynthesis", THE PLANT JOURNAL, vol. 62, no. 4, 26 February 2010 (2010-02-26), GB, pages 589 - 600, XP055818938, ISSN: 0960-7412, DOI: 10.1111/j.1365-313X.2010.04186.x
MASATAKA KAJIKAWA ET AL: "Genomic Insights into the Evolution of the Nicotine Biosynthesis Pathway in Tobacco", PLANT PHYSIOLOGY, vol. 174, no. 2, 18 April 2017 (2017-04-18), Rockville, Md, USA, pages 999 - 1011, XP055497758, ISSN: 0032-0889, DOI: 10.1104/pp.17.00070
DATABASE REFSEQ [online] NCBI; 21 October 2014 (2014-10-21), "Predicted: disease resistance protein At4g27190-lie [Nicotiana sylvestris]", XP055859872, accession no. XP_009790435 Database accession no. XP_009790435
DATABASE UniProt [online] 12 April 2017 (2017-04-12), "SubName: Full=disease resistance protein At4g27190-like {ECO:0000313|RefSeq:XP_016499100.1, ECO:0000313|RefSeq:XP_016499101.1};", XP055859864, retrieved from EBI accession no. UNIPROT:Unreviewed Database accession no. Unreviewed
DATABASE UniProt [online] 12 April 2017 (2017-04-12), "SubName: Full=disease resistance protein At4g27190-like isoform X1 {ECO:0000313|RefSeq:XP_016435313.1}; SubName: Full=disease resistance protein At4g27190-like isoform X2 {ECO:0000313|RefSeq:XP_016435318.1};", XP055859857, retrieved from EBI accession no. UNIPROT:Unreviewed Database accession no. Unreviewed
LEWIS RAMSEY S. ET AL: "Genetic and Agronomic Analysis of Tobacco Genotypes Exhibiting Reduced Nicotine Accumulation Due to Induced Mutations in Berberine Bridge Like (BBL) Genes", FRONTIERS IN PLANT SCIENCE, vol. 11, 3 April 2020 (2020-04-03), pages 1 - 14, XP055860686, DOI: 10.3389/fpls.2020.00368
KIDD SARAH K. ET AL: "The A and B Loci in Tobacco Regulate a Network of Stress Response Genes, Few of which are Associated with Nicotine Biosynthesis", PLANT MOLECULAR BIOLOGY, vol. 60, no. 5, 1 March 2006 (2006-03-01), NL, pages 699 - 716, XP055860698, ISSN: 0167-4412, Retrieved from the Internet DOI: 10.1007/s11103-005-5546-z
TODD ANDREA T. ET AL: "A functional genomics screen identifies diverse transcription factors that regulate alkaloid biosynthesis in Nicotiana benthamiana : TFs regulating nicotine biosynthesis", THE PLANT JOURNAL, vol. 62, no. 4, 26 February 2010 (2010-02-26), GB, pages 589 - 600, XP055818938, ISSN: 0960-7412, DOI: 10.1111/j.1365-313X.2010.04186.x
MASATAKA KAJIKAWA ET AL: "Genomic Insights into the Evolution of the Nicotine Biosynthesis Pathway in Tobacco", PLANT PHYSIOLOGY, vol. 174, no. 2, 18 April 2017 (2017-04-18), Rockville, Md, USA, pages 999 - 1011, XP055497758, ISSN: 0032-0889, DOI: 10.1104/pp.17.00070
DATABASE REFSEQ [online] NCBI; 21 October 2014 (2014-10-21), "Predicted: disease resistance protein At4g27190-lie [Nicotiana sylvestris]", XP055859872, accession no. XP_009790435 Database accession no. XP_009790435
DATABASE UniProt [online] 12 April 2017 (2017-04-12), "SubName: Full=disease resistance protein At4g27190-like {ECO:0000313|RefSeq:XP_016499100.1, ECO:0000313|RefSeq:XP_016499101.1};", XP055859864, retrieved from EBI accession no. UNIPROT:Unreviewed Database accession no. Unreviewed
DATABASE UniProt [online] 12 April 2017 (2017-04-12), "SubName: Full=disease resistance protein At4g27190-like isoform X1 {ECO:0000313|RefSeq:XP_016435313.1}; SubName: Full=disease resistance protein At4g27190-like isoform X2 {ECO:0000313|RefSeq:XP_016435318.1};", XP055859857, retrieved from EBI accession no. UNIPROT:Unreviewed Database accession no. Unreviewed
LEWIS RAMSEY S. ET AL: "Genetic and Agronomic Analysis of Tobacco Genotypes Exhibiting Reduced Nicotine Accumulation Due to Induced Mutations in Berberine Bridge Like (BBL) Genes", FRONTIERS IN PLANT SCIENCE, vol. 11, 3 April 2020 (2020-04-03), pages 1 - 14, XP055860686, DOI: 10.3389/fpls.2020.00368
KIDD SARAH K. ET AL: "The A and B Loci in Tobacco Regulate a Network of Stress Response Genes, Few of which are Associated with Nicotine Biosynthesis", PLANT MOLECULAR BIOLOGY, vol. 60, no. 5, 1 March 2006 (2006-03-01), NL, pages 699 - 716, XP055860698, ISSN: 0167-4412, Retrieved from the Internet
Attorney, Agent or Firm:
JONES, Elizabeth (GB)
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