Title:
MUTANT GLUTAMATE-CYSTEINE LIGASE AND METHOD FOR MANUFACTURING Γ-GLUTAMYL-VALYL-GLYCINE
Document Type and Number:
WIPO Patent Application WO/2015/115612
Kind Code:
A1
Abstract:
Provided are a variant of glutamate-cysteine ligase (gshA) suitable for production of γ-Glu-Val and a method for manufacturing γ-Glu-Val-Gly using the same. Using the gshA mutant having a specific mutation, γ-Glu-Val is produced with Glu and Val as starting materials, and furthermore, γ-Glu-Val-Gly is produced using the γ-Glu-Val and Gly as starting materials. Using the gshA mutant having a specific mutation, γ-Glu-Val-Gly is produced using Glu, Val, and Gly as starting materials.
Inventors:
SASAHARA AYAKO (JP)
TABUCHI ERI (JP)
SUZUKI HIDEYUKI (JP)
TAGAMI UNO (JP)
KASHIWAGI TATSUKI (JP)
ITO TAKAYUKI (JP)
NOZAKI HIROYUKI (JP)
SUZUKI SHUNICHI (JP)
TABUCHI ERI (JP)
SUZUKI HIDEYUKI (JP)
TAGAMI UNO (JP)
KASHIWAGI TATSUKI (JP)
ITO TAKAYUKI (JP)
NOZAKI HIROYUKI (JP)
SUZUKI SHUNICHI (JP)
Application Number:
PCT/JP2015/052741
Publication Date:
August 06, 2015
Filing Date:
January 30, 2015
Export Citation:
Assignee:
AJINOMOTO KK (JP)
International Classes:
C12N15/09; C12N1/21; C12N9/00; C12P21/02
Domestic Patent References:
| WO2013054447A1 | 2013-04-18 | |||
| WO2008126784A1 | 2008-10-23 | |||
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SUZUKI, H. ET AL., J. BACTERIOL., vol. 187, 2005, pages 5861 - 5867
KUMAGAI H. ET AL.: "y-Glutamylcysteine Synthetase from Proteus mirabilis.", AGRIC. BIOL. CHEM., vol. 46, no. 5, 1982, pages 1301 - 1309, XP055217376
BRENDA S. KELLY ET AL., J. BIOL. CHEM., vol. 277, 2002, pages 50 - 58
KUMAGAI, H. ET AL., AGRIC. BIOL. CHEM., vol. 46, 1982, pages 1301 - 1309
BLATTNER FR ET AL., SCIENCE, vol. 277, 1997, pages 1453 - 1462
ELIZABETH C. TYLER ET AL., COMPUTERS AND BIOMEDICAL RESEARCH, vol. 24, no. 1, 1991, pages 72 - 96
BARTON G.J. ET AL., JOURNAL OF MOLECULAR BIOLOGY, vol. 198, no. 2, 1987, pages 327 - 337
THOMPSON JD ET AL., NUCLEIC ACID RESEARCH, vol. 22, no. 22, 1994, pages 4673 - 4680
HIGUCHI, R.: "PCR Technology", vol. 61, 1989, STOCKTON PRESS
CARTER P., METH., IN ENZYMOL., vol. 154, 1987, pages 382
KRAMER, W.; FRITS, H.J., METH. IN ENZYMOL., vol. 154, 1987, pages 350
KUNKEL, T.A. ET AL., METH. IN ENZYMOL., vol. 154, 1987, pages 367
APPL. MICROBIOL. BIOTECHNOL., vol. 53, 2000, pages 674 - 679
JOURNAL OF BIOTECHNOLOGY, vol. 104, 2003, pages 311 - 323
APPL. ENVIRON. MICROBIOL., vol. 71, no. 12, December 2005 (2005-12-01), pages 8587 - 8596
GOLDSTEIN ET AL.: "Prokaryotic Promoters in Biotechnology", BIOTECHNOL. ANNU. REV., vol. 1, 1995, pages 105 - 128
AGRIC. BIOL. CHEM., vol. 48, 1984, pages 2901 - 2903
"Fundamental Microbiology", vol. 8, 1987, KYORITSU SHUPPAN CO., LTD, article "Genetic Engineering"
MILLER, J.H.: "Experiments in Molecular Genetics", 1972, COLD SPRING HARBOR LABORATORY
DATSENKO, K.A.; WANNER, B.L., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 6640 - 6645
MANDEL, M.; HIGA, A., J. MOL. BIOL., vol. 53, 1970, pages 159 - 162
DUNCAN, C.H.; WILSON, G.A.; YOUNG, F.E., GENE, vol. 1, 1977, pages 153 - 167
CHANG, S.; CHOEN, S.N., MOL. GEN. GENET., vol. 168, 1979, pages 111 - 115
BIBB, M.J.; WARD, J.M.; HOPWOOD, O.A., NATURE, vol. 274, 1978, pages 398 - 400
HINNEN, A.; HICKS, J.B.; FINK, G.R., PROC. NATL. ACAD. SCI. USA, vol. 75, 1978, pages 1929 - 1933
DATSENKO, K.A; WANNER, B.L., PROC. NATL. ACAD. SCI. USA, vol. 97, 2000, pages 6640 - 6645
CHO, E.H.; GUMPORT, R.I.; GARDNER, J.F., J. BACTERIOL., vol. 184, 2002, pages 5200 - 5203
HEERMANN, R ET AL., MICROBIAL CELL FACTORIES, vol. 7, 2008, pages 14
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 272, 1997, pages 8611 - 8617
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES, USA, vol. 95, 1998, pages 5511 - 5515
JOURNAL OF BIOLOGICAL CHEMISTRY, vol. 26, no. 116, 1991, pages 20833 - 20839
"Molecular Cloning", 2001, COLD SPRING HARBOR LABORATORY PRESS
HORI, H. ET AL., APPL. MICROBIOL. BIOTECHNOL., vol. 48, no. 6, 1997, pages 693 - 698
YAMAMOTO, S ET AL., BIOSCI. BIOTECHNOL. BIOCHEM., vol. 69, no. 4, 2005, pages 784 - 789
C. AUG'E; CH. GAUTHERON, TETRAHEDRON LETT., vol. 29, 1988, pages 789 - 790
MURATA, K. ET AL., AGRIC. BIOL. CHEM., vol. 52, no. 6, 1988, pages 1471 - 1477
"Amino Acid Fermentation", 30 May 1986, GAKKAI SHUPPAN CENTER LTD., pages: 77 - 100
SUZUKI, H. ET AL., J. BACTERIOL., vol. 187, 2005, pages 5861 - 5867
Attorney, Agent or Firm:
KAWAGUCHI, Yoshiyuki et al. (JP)
Yoshiyuki Kawaguchi (JP)
Yoshiyuki Kawaguchi (JP)
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