Title:
MUTANT NITRILE HYDRATASE, NUCLEIC ACID CODING SAID MUTANT NITRILE HYDRATASE, EXPRESSION VECTOR AND TRANSFORMANT INCLUDING SAID NUCLEIC ACID, PRODUCTION METHOD FOR SAID MUTANT NITRILE HYDRATASE, AND PRODUCTION METHOD FOR AMIDE COMPOUND
Document Type and Number:
WIPO Patent Application WO/2018/124247
Kind Code:
A1
Abstract:
A nitrile hydratase that is derived from Pseudonocardia thermopila and has an α subunit and a β subunit, wherein a specific amino acid residue has been substituted for the amino acid residue in at least one position selected from the group consisting of the 40th and 43rd residues from the N terminal of the α subunit, and the 205th, 206th and 215th residues from the N terminal of the β subunit.
Inventors:
TATENO TOSHIHIRO (JP)
TOKUDA JUNKO (JP)
KAI KEIICHIROU (JP)
TOKUDA JUNKO (JP)
KAI KEIICHIROU (JP)
Application Number:
PCT/JP2017/047127
Publication Date:
July 05, 2018
Filing Date:
December 27, 2017
Export Citation:
Assignee:
MITSUI CHEMICALS INC (JP)
International Classes:
C12N15/09; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12N9/88; C12P13/02; C12P21/02
Domestic Patent References:
| WO2010055666A1 | 2010-05-20 | |||
| WO2004056990A1 | 2004-07-08 | |||
| WO2015186298A1 | 2015-12-10 | |||
| WO2012169203A1 | 2012-12-13 | |||
| WO2012164933A1 | 2012-12-06 |
Foreign References:
| JP2005160403A | 2005-06-23 | |||
| JPH09275978A | 1997-10-28 |
Other References:
MIYANAGA, AKIMASA ET AL.: "Mutational and structural analysis of cobalt-containing nitrile hydratase on substrate and metal binding", EUROPEAN JOURNAL OF BIOCHEMISTRY, vol. 271, no. 2, January 2004 (2004-01-01), pages 429 - 438, XP055096861
Attorney, Agent or Firm:
NAKAJIMA, Jun et al. (JP)
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