Title:
NATURAL PROMOTERS FOR GENE EXPRESSION IN C1 METABOLIZING BACTERIA
Document Type and Number:
WIPO Patent Application WO2004037998
Kind Code:
A3
Abstract:
Genes have been identified in the Methylomonas genome that are responsive to various metabolic and growth conditions. The identified responsiveness of these genes allows for the use of their promoters in regulated gene expression in C1 metabolizing bacteria. In particular, the hps promoter, which in its native state drives the expression of 3-hexulose-6-phosphate synthase (HPS), was found to be useful for directing expression of heterolgous coding regions (e.g., crtZ) in the obligate methanotroph Methylomonas sp. 16a.
Inventors:
DICOSIMO DEANA J
PICATAGGIO STEPHEN K
SEIP JOHN E
YE RICK W
WANG TAO
NI HAO
PICATAGGIO STEPHEN K
SEIP JOHN E
YE RICK W
WANG TAO
NI HAO
Application Number:
PCT/US2003/033698
Publication Date:
August 12, 2004
Filing Date:
October 21, 2003
Export Citation:
Assignee:
DU PONT (US)
International Classes:
C07K14/195; C12N1/21; C12N15/74; (IPC1-7): C12N1/21; C12N15/74
Foreign References:
| US6331428B1 | 2001-12-18 |
Other References:
WAECHTER-BRULLA: "Methanol Oxidation Genes in the Marine Methanotroph Methylomoas sp. Strain A4", JOURNAL OF BACTERIOLOGY, vol. 175, no. 12, June 1993 (1993-06-01), pages 3767 - 3775, XP002977282
MORRIS ET AL.: "Cloning of a methanol-inducible moxF promoter and its analysis in moxB mutants of Methylobacterium extorquens AM1rif", JOURNAL OF BACTERIOLOGY, vol. 174, no. 13, July 1992 (1992-07-01), pages 4444 - 4449, XP009006511
MORRIS ET AL.: "Cloning of a methanol-inducible moxF promoter and its analysis in moxB mutants of Methylobacterium extorquens AM1rif", JOURNAL OF BACTERIOLOGY, vol. 174, no. 13, July 1992 (1992-07-01), pages 4444 - 4449, XP009006511
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