Title:
NOVEL DEOXYRIBONUCLEASE, GENE ENCODING THE SAME AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2001/012793
Kind Code:
A1
Abstract:
A novel acidic DNase (DLAD) which is an endonuclease capable of divalent cation-independently cleaving DNA under acidic conditions, sustaining its activity even in the acidic to neutral pH region and not inhibited by G-actin; a DNA encoding this enzyme; an expression vector containing this DNA; a host cell transformed by this expression vector; and medicinal compositions containing as the active ingredient the DLAD, the DLAD expression vector or the host cell transformed by the expression vector. These medicinal compositions are useful as remedies for cystic fibrosis as a substitute for DNase I and can provide a new approach to the prevention and treatment of infectious diseases.
Inventors:
TANUMA SEI-ICHI (JP)
SHIOKAWA DAISUKE (JP)
SHIOKAWA DAISUKE (JP)
Application Number:
PCT/JP2000/002893
Publication Date:
February 22, 2001
Filing Date:
May 01, 2000
Export Citation:
Assignee:
TANUMA SEI ICHI (JP)
SHIOKAWA DAISUKE (JP)
SHIOKAWA DAISUKE (JP)
International Classes:
C12N7/01; C12N9/22; C12N15/12; C12N15/55; A61K38/00; A61K48/00; (IPC1-7): C12N9/22; C12N15/12; C12N5/10; C12N7/01; A61K31/195; A61K48/00
Other References:
DAISUKE SHIOKAWA ET AL.: "DLAD, a novel mammalian divalent cation-independent endonucleoside with homology to DNase II", NUCLEIC ACIDS RESEARCH, vol. 27, no. 20, October 1999 (1999-10-01), pages 4083 - 4089, XP002930715
SEI-ICHI TANUMA ET AL.: "Cloning of a cDNA encoding a rat DNase II-like acid DNase", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 265, no. 2, November 1999 (1999-11-01), pages 395 - 399, XP002930716
DAISUKE SHIOKAWA ET AL.: "Cloning of cDNAs encoding porcine and human DNase II", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 247, no. 3, 1998, pages 864 - 869, XP002930717
SEI-ICHI TANUMA ET AL.: "Cloning of a cDNA encoding a rat DNase II-like acid DNase", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 265, no. 2, November 1999 (1999-11-01), pages 395 - 399, XP002930716
DAISUKE SHIOKAWA ET AL.: "Cloning of cDNAs encoding porcine and human DNase II", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol. 247, no. 3, 1998, pages 864 - 869, XP002930717
Attorney, Agent or Firm:
Takashima, Hajime (Hiranomachi 3-chome Chuo-ku Osaka-shi, Osaka, JP)
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