The invention relates to novel piperazinyl—bis(alkyl amino)pyrimidine derivatives of the formula

wherein two of X, Y and Z mean a nitrogen atom each and the thir one is a methine group;

R ¹ and R ² represent, independently from each other, a prim ary amino group bearing as substituent a branched-chai C4_ ₈ alkyl, -alkenyl or -alkynyl group, or a C4_ι ₀ cyclo alkyl group comprising 1 to 3 ring(s) and being option ally substituted by Cι_ ₃ alkyl group(s) ; or

R ¹ and R ² stand together for a spiro-heterocyclic secondar amino group containing at most 10 carbon atoms an optionally at least one oxygen atom as an additiona heteroatom; or one of R ¹ and R ² means an unsubstituted heterocyclic second ary amino group containing 4 to 7 carbon atoms and th other one is an above-identified primary amino group an above-identified spiro- eterocyclic secondary amin group, or a heterocyclic secondary amino group con

taining 4 to 7 carbon atoms and substituted by Cι_4 alkyl group(s); and n is 1 or 2, as well as their acid addition salts. Further, the invention relates to a process for t preparation of the above compounds.

The compounds of the formula (I) according to t invention are new and possess a significant biologica activity in themselves; however, their use in the prepara tion of lipid peroxidation-inhibiting substances bears greater importance.

Hereinafter and in the claims primary amino groups ar meant to contain a hydrogen atom as one substituent wherea the other substituent is a branched-chain C4_ ₈ alkyl, -alke nyl or —alkynyl group, or a C ₄ _ιo ^c Y ^cl - ^oa lkyl group, compris ing 1 to 3 rings, and being optionally substituted by C1--. ₃ alkyl group(s) . The branched-chain C4_ ₈ alkyl, -alkenyl a —alkynyl groups may be various iso-, sec— and te t-butyl butenyl, pentyl, pentenyl, pentynyl, hexyl, hexenyl, hexy nyl, pentyl, heptenyl, heptynyl, octyl, octenyl and octyny groups. Preferred representatives of these are the 1,1—di methylethyl, 2,2—dimethylpropyl and 4,4— imethyl—1—penten—5 —yl groups.

The C4_ιo ^c y ^c loalkyl group comprising 1 to 3 rings a being optionally substituted by Cι_3alkyl group(s) can e.g. a cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl o adamantyl group. These groups may be unsubstituted or bea one or more methyl, ethyl or propyl group(s) as substi tuents. As R ¹ and R ² , the spiro-heterocyclic secondary ami group corresponding at most 10 carbon atoms and optionall at least one additional oxygen heteroatom is exemplified the 4,4—ethylenedioxy—1—piperidinyl group, without any limi tation thereto. When representing an unsubstituted heterocyclic secon ary amino group containing 4 to 7 carbon atoms, one of

and R ² may preferably be a pyrrolidino, piperidino or aze- pino group. In this case, the other one of R ¹ and R ² means either a primary amino group mentioned above, or an above- defined secondary heterocyclic group having spiro structure, or an above-defined heterocyclic secondary amino group con¬ taining 4 to 7 carbon atoms and substituted by Cι_ ₄ alkyl group(s) . These Cι_4alkyl groups may be the same or differ¬ ent, e.g. methyl, ethyl, n- or isopropyl, or n-, iso-, sec- or tert—butyl groups. A preferred representative of these substituted heterocyclic secondary amino groups is e.g. the 2,2,6,6—tetramethyl—1—piperidiny1 group.

Lipid peroxidation occurring as a consequence of injuries is a secondary process. Some cells are immediately destroyed when the tissues are damaged. During the next hours the injury is extended to the surrounding cells. This is induced by free oxygen radicals which attack the lipid layer of the cellular membrane and may eventually lead to cell death by damaging the membrane and releasing hydrogen peroxide. Compounds inhibiting the lipid peroxidation can prevent this secondary process occurring as sequels of e.g. paralyses, cephalic or spinal traumas. Compounds possessing such an effect may be utilized e.g. for the treatment of Alzheimer's disease, muscular dystrophy and the like.

The following publications discussing the preparation of piperazinylpyrimidine derivatives are known; the prepara¬ tion of antiinflammatory 2,4—diamino—6—piperazinylpyrimidine derivatives is described in the GB patent specification No. 1,345,640. In the target compounds the amino groups, being the same or different, may be monoalkylamino groups con- taining 1 to 6 carbon atoms, cycloalkylamino groups contain¬ ing at most 6 carbon atoms or a orpholino group whereas the amino group in position 4 may be a piperazino group, too.

According to this patent specification the starting substance of the synthesis is 2,4,6—trichloropyrimidine of the formula (IV) (see hereinafter) which is first reacted with morpholine, then the dichloro compound obtained is

brought into reaction with ethylamine, finally the mon chloro compound formed is reacted with piperazine to give —ethylamino-2—morpholino-6-(l-piperazinyl)pyrimidine as described in the examples. Compounds containing a morpholi group as one of the two amino groups and a cycloalkylami or monoalkylamino group containing at most 4 carbon ato are indicated to be most effective. Among these in the pr ferred compounds one of the two amino groups is ethylami or cyclopropylamino group, the other one is a morpholi group. Detailed examples with physical characteristics a given for the preparation of 2—ethylamino—4—morpholino—6-( —piperazinyl)pyrimidine and 4—cyclopropylamino—2—morpholin —6—(1—piperaziny1)pyrimidine, too.

In the published German patent specification N 2,630,140 2,4—diaminopyrimidine derivatives are describe wherein the amino groups are mono- or disubstituted; t substituents may be Cι_4alkyl, C ₂ _4alkenyl or cycloprop groups. post-emergent herbicidal activity is attributed these compounds. The synthesis of [piperazinyl-bis(amino)pyrimidinyl steroids of primarily lipid peroxidation-inhibiting activi is described in the published PCT patent application No. 87/01706. There are given examples for the preparation piperazinyl—bis(alkylamino)pyrimidine derivatives used starting substances for the steroid derivatives mention above, too. Thus, 2,4,6—trichloropyrimidine is react primarily with saturated or unsaturated amines containing to 3 carbon atoms as well as pyrrolidine, morpholine, hex methyleneimine or N—methylpiperazine. According to t biological data published in this application, 4—(1-piper zinyl)—2,6—bis(pyrrolidino)pyrimidine proved to be the mo favourable one for the preparation of lipid peroxidatio inhibiting compounds.

It is known from the published PCT patent applicati No. WO P 91/11453 that the strength of the lipid peroxi ation-inhibiting effect is significantly influenced by usi

some piperazinyl-bis(alkylamino)pyrimidine derivatives as substituents connected e.g. to compounds having steroid skeleton or other substances having a similar structure.

The present invention is aimed at the preparation of analogous piperazinylpyrimidine derivatives which, when used as substituents, are capable of increasing the biological activity and decreasing the toxicity of the molecules sub¬ stituted by these substituents, in comparison to known analogues. Surprisingly, it has been found that the compounds of the formula (I) according to the invention are excellent for this purpose.

The piperazinylpyrimidine compounds according to the invention can be used as substituents for various compounds, e.g. pregnane derivatives.

The novel piperazinyl—bis(alkylamino)pyrimidine deriva¬ tives of the formula (I) can be prepared in such a way that a) about 1 mole of a 2,4,6-trichloropyrimidine charac¬ terized by formula

wherein X, Y and Z are as defined above, is reacted with about 1 mole of a primary or secondary amine of the formula R ¹ —H, wherein R ¹ is as defined above, the obtained isomeric mixture of a 2—amino—4,6—di- chloropyrimidine of the formula

and a 4—amino—2,6-dichloropyrimidine of the formula

wherein R ¹ is as defined above, is separated to t individual isomers, and about 1 mole of a thus obtained individual isomer reacted with about 1 mole of a primary or secondary amine the formula R ² —H, wherein R ² is as defined above, then after separating the isomeric mixture of diaminochlor pyrimidines of the formula

and formula

wherein R ¹ and R ² are as defined above, obtained from a 4- —amino—2,6-dichloropyrimidine of the formula (Illb) , wherein R ¹ is as defined above, to the individual isomers, one of these individual isomers or a 2,6-diamino-4- —chloropyrimidine of the formula

wherein R ¹ and R ² are as defined above, obtained from a 2- —amino—4,6-dichloropyrimidine of the formula (Ilia), is reacted with a piperazine derivative of the formula

wherein n is 1 or 2; or b) about 1 mole of a 2,4,6—trichloropyrimidine charac¬ terized by the formula (IV) , wherein X, Y and Z are as defined above, is reacted with about 2 moles of a primary or secondary amine of the formula R ¹ -!., wherein R ¹ is as defined above, then after separating the obtained, isomeric mixture of di-

a inochloropyrimidines of the formulae (Ilba) (Ilbb wherein R ¹ and R ² are the same as R ¹ defined above, to t individual isomers, one of these individual isomers reacted with a piperazine derivative of the formula (V wherein n is 1 or 2, in order to obtain piperazinyl-bi (alkylamino)pyri idine derivatives of the formula (I) bea ing identical substituents as R ¹ and R ² , wherein X, Y, R ¹ , R ² and n are as defined above, and, if desired, a piperazinyl-bis(alkylamino)pyrim dine derivative of the formula (I) obtained, wherein X, Z, R ¹ , R ² and n are as defined above, is converted into acid addition salt by reacting it with an acid and/or a fr base of the formula (I) is liberated from its acid additi salt. 2,4,6—Trichloropyrimidine used as starting substance the process of the invention is a well-known compound [Be 33, pages 3666 (1900) and 3_7, page 3657 (1904) ; Chem. Abst Registration No. 3764—01—0]. The primary and seconda amines of the formulae R ¹ —H and R ² —H, as well as the piper zine derivatives of the formula (V) are commercially avai able products.

Depending on the reactivity of the amine, the reacti of a 2,4,6-trichloropyrimidine of the formula (IV) wi primary or secondary amines of the formula R ¹ —H may carried out at a temperature between —20°C and +40°C with reaction time of from 30 minutes up to several days. Wh using the sterically hindered 2,2,6,6—tetramethylpiperidi (which may be used as a solvent, too) a boiling under refl for about 50 hours are necessary at the boiling point of t reaction mixture for completing the reaction. The working- of the reaction mixture as well as the recovery of t product are preferably carried c:t in such a manner th after termination of the reaction the solvent is distill off, the residue is dissolved in a halogenated solvent, pr ferably chloroform, then the solution is washed first wi aqueous sodium hydroxide solution and subsequently wi

- 9 - .

water. After separation the organic phase is dried, the sol vent is distilled off, then the two alkylamino—dichloropyri midine isomers formed in the reaction are separated by chro matography on a silica gel column. The separated individua isomers are purified by recrystallization.

After separation the iso eric alkylamino—dichloropyri midine derivatives of the formula (Ilia) and (Illb) obtaine in the first step are again reacted with the same or differ ent amine. The parameters of this reaction are mainl dependent on the reactivity of the amine reactant. Thus, th reaction can be made complete at room temperature when pyr rolidine is used as an amine; whereas a reaction lasting fo about 15 hours at 130°C is needed in the case of tert-butyl amine. The reaction of neopentylamine with the isomeri alkylamino—dichloropyrimidine derivatives can be carried ou under milder conditions: this reaction becomes complete b boiling in isopropanol for about 20 hours. The less reactiv 5—amino—4,4—dimethyl—1—pentene reacts with the isomeri alkylamino-dichloropyrimidine derivatives only at highe temperatures. Due to its large space demand, 1—amino—adaittan tane should be reacted by boiling in n—butanol for about 7 hours.

The recovery of the individual isomers from reactio mixtures containing the diaminochloropyrimidine isomers o the formulae (Ilba) and (Ilbb) , formed from 4—amino—2,6—di chloropyrimidine of the formula (Illb) in the second step can be achieved by using e.g. the method described above fo the recovery of compounds of the formulae (Ilia) and (Illb)

According to the invention the preparation of piperazi nylpyrimidine derivatives of the formula (I) by reactin bis(alkylamino)—chloropyrimidine derivatives of formula (Ila) , (Ilbb) or (Ilba) with piperazine derivatives of for mula (V) is suitably carried out as described hereinafter.

The bis(alkylamino)—chloropyrimidine derivative of th formula (Ila) or (Ilbb) or (Ilba) is dissolved in a tertiar amine, preferably N-ethylmorpholine, and boiled with a

excess of the piperazine derivative of the formula (V) und nitrogen for about 25 hours. After the reaction has beco complete, N-ethylmorpholine used as solvent and the maj part of the excess of the piperazine derivative of the fo mula (V) are distilled off, water is added to the resid and distilled off again. This repeated distillation is co tinued under atmospheric pressure until the head temperatu reaches about 100°C. After dissolving the residue in chlor form the solution is washed first with aqueous sodium hyd oxide solution, then with water. After separation t organic phase is dried and the chloroform is distilled of The residue is purified first by chromatography on a sili gel column and then by recrystallization.

The advantage of using the piperazinyl-bis(alkylamino pyrimidine derivatives of the formula (I) for the prepar tion of lipid peroxidation-inhibiting compounds is demo strated on 21—{4-[2,4-bis(adamantylamino)—6—pyrimidinyl]- -piperazinyl}—16α-methylpregna-l,4,9(11)triene-3,20-dione methanesulfonate. The pharmacological study was carried out on unane thetized mice by using a known experimental cephalic trau model [J. Neurosurg. _ 2. _. page 882 (1980)] modified by us. this study, the potential cerebroprotective effects intravenous (i.v.) doses of the compounds were investigate A metal cleaver of defined weight was let fall onto defined part of the scullcap surface of the experiment animals from a defined height under the force of gravit Within 5 minutes following the closed cephalic inju induced by the cleaver, a suitable dose of the substan under test was injected to a tail vein of the animals a the neurological condition of the animals was evaluated the 60th minute following the cephalic trauma. This eval ation was performed by using a simple grip test, examination of the intactness or affectedness of the mot functions of. both the upper and lower limbs. In additio the frequency of cases considered to be "mild" or "severe

- 11 -.

based on pretermined criteria, as well as the ratio o animals suffering from paraparesis-paraplegia were regis tered in the various treatment groups. The development o eventually occurring deficiency symptoms of the nervou system was made quantitative by comparison of the neuro logical condition of animals treated with the active agen to the condition of controls treated only with the vehicle.

When administered in the most favourable dose of 0. mg/kg ² -■— i4—[2,4-bis(adamantylamino)-6-pyrimidiny1]-1-pipe razinyl}—16α—methylpregna—1,4,9(ll)triene—3,20—di one methanesulfonate increased by 33% the number of cases signe as "mild" (based on the neurological symptoms induced by th cephalic trauma) and similarly, it decreased by 33% th frequency of cases involving paraparesis-paraplegia. Th known tirilazad mesylate (see the published PCT paten application No. WO 87/01706) , chemically 16α-methyl-21-{4 —[2,4—bis(pyrrolidino)-6—pyrimidinyl]—1—piperaziny 1}pregna- —1,4,9(ll) ^N triene—3,20—dione methanesulfonate, was used a control. When administered in the most effective dose of 0. mg/kg, tirilazad mesylate increased only by 23% the numbe of animals showing "mild" deficiency symptoms and decrease only by 20% the frequency of paraplegic animals.

Thus, it is obvious from the experimental results tha the compounds according to our invention increase the lipi peroxidation-inhibiting effect of the basic substance to higher grade in comparison to known compounds having similar structure.

The invention is illustrated in detail by the aid o the following non-limiting Examples. Example 1

Preparation of 4,6—dichloro—2— yrrolidinopyrimidine and 2,4 —dichloro-6-pyrrolidinopyrimidine

After dropping 23.7 ml (286.6 mmoles) of pyrrolidine t a mixture containing 25.0 g (136.3 mmoles) of 2,4,6-trichlo ropyri idine in 200. ml of tetrahydrofuran at -20°C withi about 30 minutes, the cooling is stopped and after stirrin

- 12 -

for additional 30 minutes the reaction mixture is evapo ated. After distributing the residue between 500 ml chloroform and 50 ml of 10% sodium hydroxide solution, t organic phase is separated, washed 4 times with 150 ml o water each, then dried and evaporated. The residue i subjected to chromatography on a silica gel column. By usi a 19:1 hexane/ethyl acetate mixture as eluent the eluate i evaporated and the evaporation residue is recrystallize from hexane to give 7.51 g (25.27%) of 4,6-dichlorc—2-pyrro lidinopyrimidine, m.p. 95-98°C. ^l -H-NMR (60 MHZ, CDC1 ₃ ) S ppm: 6.51 (s, 1H, 5-H) .

By further elution with a 4:1 mixture of the above sol vent system 2,4—dichloro—6—pyrrolidinopyrimidine as th more polar product is obtained in a yield of 20.22 (68.03%) after recrystallization from hexane, m.p.: 100.5 103.5°C. ^l -H-NMR (60 MHZ, CDCI ₃ ) S ppm: 6.18 (s, 1H, 5-H).

Example 2 Preparation of 4,6—dichloro— 2—(1,1—dimethylethylamino)pyri midine and 2,6—dichloro—4—(1,1-dimethylethylamino)pyrimidi

25 g (136.6 mmoles) of 2,4,6—trichloropyrimidine ar dropwise added to a mixture of 31.52 ml of 1—amino—1,1—di methylethane and 200 ml of tetrahydrofuran at a temperatur between 10°C and 15°C while cooling and stirring. The reac tion mixture is stirred at room temperature for additional hours, ther evaporated. After distributing the residu between .50 ml of chloroform and. 50 ml of 10% sodium hydr oxide solution and separating, the organic phase is washed times with 150 ml of water each, then dried and evaporated The residue is separated by chromatography on a silica ge column by using mixtures of hexane and ethyl acetate a eluent. By using 9:1 hexane/ethyl acetate mixture 4,6-di chloro— 2—(1,1-dimethylethylamino)pyrimidine is eluate which is recrystallized from hexane to obtain a yield o 11.35 g (37.84%), m.p.: 70-74°C. -H-NMR (60 MHz, THF-d ₈ ) <S ppm: 6.63.(s, 1H, 5-H).

By continuing the elution with a 4:1 mixture of the above solvent system 2,6—dichloro—4—(1,1—dimethylethy1- amino)pyrimidine as a more polar product is obtained, which is recrystallized from ethyl acetate to give 13.31 g (44.35%) of pure product, m.p. :192-195°C. ^l -H-NMR (60 MHz, THF-d ₈ ) δ ppm: 6.32 (s, 1H, 5-H).

Example 3 Preparation of 4—chloro—2,6—bis(1,1—dimethylethylamino)pyri- idine A solution containing 5.0 g of 4,6-dichloro—2—(1,1-di- methylethylamino)pyrimidine in 25 ml of 1—amino—1,l—di- methylethane is heated in a closed tube at 130°C for 15 hours. Thereafter, the reaction mixture is evaporated and the evaporation residue is distributed between 80 ml of chloroform and 15 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 20 ml of water each, then dried and evaporated. The evaporation resi¬ due is recrystallized from hexane to give the title compound in a yield of 5.45 g (93.4%), m.p.: 128-130°C. ^l -H-NMR (60 MHz, THF-d ₈ ) S ppm: 5.67 (s, 1H, 5-H). Example 4 Preparation of 2,4—bis(1,1—dimethylethylamino)—6—(1—pipera- ziny1)pyrimidine

A mixture containing 9.58 g (34.7 mmoles) of 4—chloro- —2,6-bis(1,1—dimethylethylamino)pyrimidine, 11.95 g (138.8 mmoles) of piperazine and 150 ml of N—ethylmorpholine is boiled under reflux and nitrogen atmosphere for 25 hours, then the solvent and the excess of piperazine are distilled off under atmospheric pressure. Subsequently, 100 ml of water are added to the residue and the distillation is con¬ tinued until the head temperature reaches 100°C. After cool¬ ing down, the residue is distributed between 200 ml of chloroform and 30 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 50 ml of water each, then dried and evaporated. The residue is purified by chromatography on a silica gel column by using a

9:1 mixture of chloroform/methanol as eluent. After evapor¬ ating the eluate the residue is recrystallized from hexane to obtain the title compound in a yield of 64%, m.p.: 142- 145°C. ^l -H-NMR (60 MHz, CDC1 ₃ ) <5 ppm: 4.99 (s, 1H, 5-H). Example 5 Preparation of 6—chloro—2,4—bis(1,1—dimethylethylamino)pyri- idine and 2—chloro—4,6—bis(1,1—dimethylethylamino)pyrimi¬ dine A solution of 10 g of 2,6—dichloro—4—(1,1—dimethy1- ethylamino)pyrimidine in 50 ml of 1-amino—1,1-dimethylethane is heated in a closed tube at 130°C for 15 hours. There¬ after, the reaction mixture is evaporated and the residue is distributed between 150 ml of chloroform and 30 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 10 ml of water each, then dried and evaporated. The evaporation residue is subjected to chromatography on a silica gel column by using a 9:1 mixture of hexane/ethyl acetate as eluent to give 6—chloro—2,4—bis- (1,1—dimethylethylamino)pyrimidine which is recrystallized from hexane to result in a yield of 9.92 g (85.1%), m.p.: 125-127°C. By continuing the elution with a 4:1 mixture of hexane/ethyl acetate 2—chloro—4,6—bis(1,1—dimethylethyla i¬ no)pyrimidine as a more polar product is obtained which is recrystallized from hexane to result in a yield of 0.49 g (4.2%) , m.p.: 132-135°C. ^-H-NMR (60 MHz, CDCI ₃ ) δ ppm: 4.93 (s, 1H, 5-H).

Example 6 Preparation of 4,6—bis(1,1—dimethylethylamino) —2—(1—pipera- ziny1)pyrImidine

By reacting 2—chloro—4,6—bis(1,1—dimethylethylamino)- pyrimidine with piperazine as described in Example 4, the title product is obtained in a yield of 76.5%, m.p.: 135- 138°C. l-B-NMR (60 MHZ, CDCI3) S ppm: 4.83 (S, 1H, 5-H).

Example 7 Preparation of 1—[2,4-bis(1,1—dimethylethylamino)—6—pyrimi- dinylJ—hexahydro—IH—1,4-diazepine

By reacting 4—chloro—2,6—bis(1,1—dimethylethylamino)- pyrimidine with hexahydro—IH—1,4—diazepine as described in Example 4, the title product is obtained in a yield of 55.3%, m.p.: 127-132°C.

!H-NMR (60 MHz, CDC1 ₃ ) δ ppm: 4.96 (s, IH, 5-H). Example 8 Preparation of 6—chloro— 2—(1,1—dimethylethylamino)—4—(2,2- —dimethyIpropylamino)pyrimidine

After dissolving 5.0 g of 4,6—dichloro— 2—(1,1—dimethy1- ethylamino)pyrimidine in 25 ml of isopropanol and adding 5 ml of 1—amino—2,2—dimethylpropane the reaction mixture is boiled under reflux for 20 hours. Then, the reaction mixture is evaporated and the residue is distributed between 80 ml of chloroform and 15 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 20 ml of water each, then dried and evaporated. After recrys- tallizing the evaporation residue from hexane the title product is obtained in a yield of 4.49 g (73%), m.p.: 109.5- 111°C. ⁱ H-NMR (60 MHz, CDCI ₃ ) δ ppm: 5.71 (s, IH, 5-H). Example 9 Preparation of 2—(1,1— imethylethylamino)-4—(2,2-dimethy1- propylamino)—6—(1— iperazinyl)pyrimidine

By reacting 6—chloro—2—(1,1—dimethylethylamino)—4—(2,2- —dimethyIpropylamino)pyrimidine with piperazine as described in Example 4, the title product is obtained in a yield of 86.0%, m.p.: 120-124°C. ⁱ H-NMR (60 MHz, CDCI ₃ ) <S ppm: 4.96 (s, IH, 5-H).

Example 10 Preparation of 6—chloro—4—(1,1—dimethylethylamino) —2—(2,2- —dimethyIpropylamino)pyrimidine After dissolving 5.0 g of 2,4—dichlorc—6-(l,1-dimethyl- ethylamino)pyrimidine in 25 ml. of isopropanol and adding 5

ml of 1—amino—2,2—dimethylpropane, the reaction mixture is boiled under reflux for 20 hours. Subsequently, the reaction mixture is evaporated and the residue is distributed between 80 ml of chloroform and 15 ml of 10% sodium hydroxide solu- tion. After separation the organic phase is washed 4 times with 20 ml of water each, then dried and evaporated. The evaporation residue is purified by chromatography on a silica gel column by using a 9:1 mixture of hexane/ethyl acetate as eluent. After recrystallizing the eluted product from hexane, the title product is obtained in a yield of 4.05 g (65.9%), m.p.: 115-119°C. l-H-NMR (60 MHz, CDC1 ₃ ) 5 ppm: 5.66 (s, IH, 5-H).

Example 11 Preparation of 4—(1,1— imethylethylamino) —2—(2,2-dimethyl- propylamino)—6—(1—piperazinyl)pyrimidine

The reaction of 4—chloro—6-(l,1-dimethylethylamino)-2- —(2,2—dimethyIpropylamino)pyrimidine with piperazine as described in Example 4 gives the title product in a yield of 82.3%, m.p.: 146-148°C. ^l -H-NMR (60 MHz, CDCI ₃ ) <S ppm: 5.02 (s, IH, 5-H). Example 12 Preparation of 4—chloro—2—(1,1—dimethylethylamino)—6—pyrro- lidinopyrimidine ^~

After adding 10 g of 4,6—dichloro—2-(l,1—dimethylethy1- amino)pyrimidine in small portions to 40 ml of pyrrolidine at a temperature below 10°C while cooling and stirring, the reaction mixture is stirred at room temperature for 1 hour and then evaporated. The residue is distributed between 150 ml of chloroform and 30 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 50 ml of water each, then dried and evaporated. After recrys¬ tallizing the evaporation residue from ethyl acetate the title product is obtained in a yield of 10.76 g (93%), m.p. :153-157°C. ^-H-NMR (60 MHz, CDCI ₃ ) δ ppm: 5.67 (s, IH, 5-H).

Example 13 Preparation of 2—(1,1—dimethylethylamino)—4—(1—piperaziny1)- -6-^yrrolidinopyrimidine

The reaction of 4—chloro—2—(1,1—dimethylethylamino)—6- -pyrrolidinopyrimidine with piperazine as described in Example 4 gives the title product in a yield of 78.1%, m.p.: 162-165°C.

^-H-NMR (60 MHz, CDCl ₃ ) δ ppm: 4.87 (s, IH, 5-H). Example 14 Preparation of 4-chloro—6—(1,1-dimethylethylamino) —2—pyrro¬ lidinopyrimidine

By reacting 2,4-dichloro—6-(1,1-dimethylethylamino)- pyrimidine with pyrrolidine as described in Example 12, the title product is obtained in a yield of 73.8%, m.p.: 148- 150°C. ⁱ H-NMR (60 MHz, CDCI ₃ ) δ ppm: 5.62 (s, IH, 5-H).

Example 15 Preparation of 4—(1,1—dimethylethylamino)—6—(1—piperaziny1)- —2-pyrrolidinopyrimidine 4—Chloro—6—(1,1-dimethylethylamino)—2—pyrrolidinop yri¬ midine is reacted with piperazine as described in Example 4 to give the title product in a yield of 67.5%, m.p.: 140- 145°C. -H-NMR (60 MHz, CDCI ₃ ) δ ppm: 4,90 (s, IH, 5-H). Example 16

Preparation of 4,6—dichloro—2—(2,2—dimethyIpropylamino)pyri¬ midine and 2,6—dichloro—4—(2,2—dimethyIpropylamino)pyrimi¬ dine

After dropwise adding 25 g (136.3 mmoles) of 2,4,6-tri- chloropyrimidine to the solution of 23.84 g (273.5 mmoles) of 1—amino—2,2—dimethyIpropane in 200 ml of tetrahydrofuran at a temperature between 10°C and 15°C under cooling and stirring, the reaction mixture is stirred at room tempera¬ ture for additional 30 minutes, then evaporated. The evaporation residue is distributed between 300 ml of chloro¬ form and 50 ml of 10% sodium hydroxide solution. After sepa-

ration the organic phase is washed 4 times with 100 ml of water each, then dried and evaporated. The evaporation resi¬ due is subjected to chromatography on a silica gel column by using mixtures of hexane and ethyl acetate as eluent. By elution with a 19:1 mixture of hexane/ethyl acetate 4,6-di- chloro—2-(2,2-dimethyIpropylamino)pyrimidine is obtained which is recrystallized from a mixture of ether and hexane to give a yield of 13.60 g (42.6%), m.p.: 63-66°C. ⁱ H- MR (60 MHz, CDC1 ₃ ) δ ppm: 6.60 (s, IH, 5-H). By further elution with a 6:1 mixture of hexane/ethyl acetate 2,6—dichlorc—4-(2,2—dimethyIpropylamino)pyrimidine is obtained, which is recrystallized from a mixture of ether and hexane to result in a yield of 14.24 g (44.6%), m.p.:77- 79°C. ¹ H-NMR (60 MHZ, CDCI ₃ ) ό ^" ppm: 6.33 (s, IH, 5-H).

Example 17 Preparation of 4-chloro-2,6—bis(2,2-dimethyIpropylamino)py¬ rimidine

The reaction of 4,6-dichlorc—2—(2,2-dimethylpropylami- no)pyrimidine with 1—amino—2,2—dimethyIpropane as described in Example 10 affords the title product in a yield of 49.4%, m.p. : 95-98°C. ^-H-NMR (60 MHz, CDCI ₃ ) δ ppm: 5.71 (s, IH, 5-H).

Example 18 Preparation of 2,4—bis(2,2—dimethyIpropylamino)—6— (1—pipera- zinyl)pyrimidine

The reaction of 4-chlorc—2,6—bis(2,2-dimethyIpropylami¬ no)pyrimidine with piperazine as described in Example 4 gives the title product in a yield of 51.5%, m.p.: 138- 140°C.

^-H-NMR (60 MHz, CDCI3) δ ppm: 4.98 (s, IH, 5-H).

Example 19 Prepara ion of 6— chloro-2 , 4— bis ( 2 , 2-dimethy Ipropylamino ) py¬ rimidine and 2— chloro-4 , 6— bis ( 2 , 2— dimethy Ipropylamino ) pyr i- idine

After dissolving 5.0 g of .2 , 4-dichlorc—6- ( 2 , 2-dimethy 1-

propylamino)pyrimidine in 25 ml of isopropanol and adding 5 ml of 1—amino—2,2-dimethyIpropane, the reaction mixture is boiled under reflux for 20 hours, then evaporated. The evap¬ oration residue is distributed between 80 ml of chloroform and 15 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 20 ml of water each, then dried and evaporated. The residue is purified by chromatography on a silica gel column by using mixtures of hexane and ethyl acetate as eluent. By eluting with a 99:1 mixture 4-chlorc—2,6-bis(2,2-dimethylpropylamino)pyrimidine is obtained, which is recrystallized from hexane to result in a yield of 5.00 g (82.3%), m.p.: 95-97°C.

By further elution with a 19:1 mixture of the above solvent system 2-chlorc—4,6-bis(2,2-dimethyIpropylamino)py- rimidine as a more polar product is obtained, which is recrystallized from hexane to give a yield of 0.30 g (4.9%), m.p.: 178-185°C.

Example 20 Preparation of 4,6—bis(2,2—dimethyIpropylamino)—2—(1—pipera- ziny1) yrimidine

The reaction of 2—chloro—4,6—bis(2,2—dimethyIpropylami¬ no)pyrimidine with piperazine as described in Example 4 leads to the title product in a yield of 78.3%, m.p.: 132- 136°C. ^-H-NMR (60 MHz, CDC1 ₃ ) <5 ppm: 4.85 (s, IH, 5-H).

Example 21 Preparation of 4—chloro—2—(2,2—dimethyIpropylamino)—6—pyrro- 1idinopyrimidine

The reaction of 4,6-dichloro—2-(2,2-dimethylpropylami- no)pyrimidine with pyrrolidine as described in Example 12 gives the title product in a yield of 96.7%, m.p.: 147- 150°C. ⁱ H-NMR (60 MHz, CDCI ₃ ) δ ppm: 5.67 (s, IH, 5-H).

Example 22 Preparation of 2—(2,2-dimethylpropylamino)—4—(1—piperazi- nyl) —6— yrrolidinopyrimidine

By reacting 2—(2,2—dimethyIpropylamino)-4—chloro—6-pyr- rolidinopyrimidine with piperazine as described in Example 4, the title compound is obtained in a yield of 76%, m.p.: 118-120°C. l-H-NMR (60 MHz, CDC1 ₃ ) δ ppm: 4.83 (s, IH, 5-H). Example 23 Preparation of 4—chloro—6—(2,2-dimethyIpropylamino)—2—pyrro¬ lidinopyrimidine

The reaction of 2,4-dichlorc—6-(2,2-dimethyIpropylami¬ no)pyrimidine with pyrrolidine as described in Example 12 affords the title compound in a yield of 75.0%, m.p.: 130- 135°C.

3-H-NMR (60 MHZ, CDCI ₃ ) ^s PP ^{m: 5} - ⁷² ( ^s » ^1H ' 5-H).

Example 24 Preparation of 4—(2,2—dimethyIpropylamino)—6—(1—piperazi- nyl)—2— yrrolidinopyrimidine The reaction of 4—chloro-6-(2,2-dimethylpropylamino) —2-

—pyrrolidinopyrimidine with piperazine as described in Example 4 gives the title compound in a yield of 76.0%, m.p. : 140-145°C. ⁱ H-NMR (60 MHz, CDCI ₃ ) δ ppm: 4.93 (s, IH, 5-H). Example 25

Preparation of 4,6—dichloro—2—[(4,4—dimethyl—1—penten—5—yl) - amino]pyrimidine and 2,6—dichloro—4—[(4,4—dimethy1—1—penten- —5—yl)amino]pyrimidine

4.59 (25 mmoles) of 2,4,6-trichloropyrimidine are added to a solution of 6.23 g (55 mmoles) of 5—amino— ,4—dimethy1- —1—pentene in 50 ml of tetrahydrofuran at room temperature, the reaction mixture is stirred at the same temperature for 4 hours, then evaporated. The evaporation residue is distri¬ buted between 60 ml of chloroform and 5 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 10 ml of water each, then dried and

evaporated. The evaporation residue is subjected to chro¬ matography on a silica gel column by using mixtures of hexane and ethyl acetate as eluents. Elution with a 19:1 hexane/ethyl acetate mixture gives 4,6—dichloro— 2—[ (4,4-di- methyl-l-penten-5-yl)amino]pyrimidine as an oily product in a yield of 2.62 g (40.3%). ^-N R (60 MHz, CDC1 ₃ ) <5 ppm: 6.61 (s, IH, 5-H).

By continuing the elution with a 9:1 mixture of hexane/ethyl acetate 2,6-dichloro—4-[ (4,4-dimethyl-l-penten- —5—yl)amino]pyrimidine as a molar polar product is obtained as an oil in a yield of 2.99 g (45.9%). ^l -H-NMR (60 MHz, CDCI ₃ ) δ ppm: 6.34 (s, IH, 5-H).

Example 26 Preparation of 4-chloro-2,6—[(4,4-dimethyl—1—penten—5—yl)- amino]pyrimidine

After adding 2,29 (20.2 mmoles) of 5—amino—4,4-di¬ methyl—1—pentene to a solution containing 2.5 g (9.61 mmoles) of ^s 4,6-dichlorc—2-[ (4,4—dimethyl-l-penten-5-yl)ami- no]pyrimidine in 25 ml of n-butanol, the reaction mixture is boiled under reflux for 10 hours, then evaporated. The evaporation residue is distributed between 50 ml of chloro¬ form and 5 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 10 ml of water each, then dried and evaporated. The evaporation resi- due is purified by chromatography on a silica gel column. By using a 19:1 mixture of hexane/ethyl acetate the title com¬ pound is obtained as an oily product in a yield of 2.25 g (69.5%) . ^l -H-NMR (60 MHz, CDCI ₃ ) ό" ppm: 5.70 (s, IH, 5-H). Example 27

Preparation of 2,4—bis[(4,4—dimethy1—1—penten—5—y1)amino]—6- —(1— iperazinyl)pyrimidine

The reaction of 4—chloro—2,6—bis[ (4,4—dimethyl—1-pen- ten—5—yl)amino]pyrimidine with piperazine as described in Example 4 gives the title compound in a yield of 73.2%, m.p.: 72-84°C.

- 22 -

ⁱ H-N R (60 MHz, CDCl ₃ ) δ ppm: 4.83 (s, IH, 5-H).

Example 28 Preparation of 2—(1—adamantylamino)—4,6—dichloropyrimidine and 4—(1—adamantylamino)—2,6—dichloropyrimidine After adding 40.6 g (225.6 mmoles) of 2,4,6-trichloro- pyrimidine to a solution of 70.3 g (465.6 mmoles) of 1—ami- noadamantane in 650 ml of tetrahydrofuran, the reaction mix¬ ture is stirred for 24 hours, then the precipitated crys¬ talline 1—aminoadamantane hydrochloride precipitated is fil- tered off and the filtrate is evaporated. By subjecting the residue to chromatography on a silica gel column by using a 49:1 mixture of hexane/acetone 2—(1—adamantylamino)—4,6—di- chloropyrimidine is obtained which is recrystallized from hexane to result in a yield of 28.74 g (43.5%), m.p.: 151- 155°C. ^l -H-NMR (60 MHZ, CDCI3) δ ppm: 6.55 (s, IH, 5-H).

By continuing the elution with a 24:1 mixture of hexane/acetone 4—(1—adamantylamino)—2,6—dichloropyrimidine as the more polar product is obtained which is recrys- tallized similarly from hexane to result in a yield of 35.56 g (53.8%), m.p.: 193-196°C. ⁱ H-NMR (60 MHz, CDCI ₃ ) S ppm: 6.33 (s, IH, 5-H).

Example 29 Preparation of 2,4—bis(1—adamantylamino)—6—chloropyrimidine The solution of 26.0 g (87.25 mmoles) of 2-(l-adaman- tylamino)—4,6—dichloropyrimidine and 39.5 g (261.6 mmoles) of 1—aminoadamantane in 200 ml of n—butanol is boiled under reflux for 75 hours, then evaporated. The evaporation resi¬ due is suspended in 400 ml of ether, filtered off, and the precipitate is dried, then purified by chromatography on a silica gel column, by using chloroform as eluent. The obtained product is recrystallized from ether to obtain the title compound in a yield of 23.94 g (66.44%), m.p.: 232- 236°C. -H-NMR (60 MHz, CDCl ₃ ) δ ppm: 5.64 (s, IH, 5-H).

Example 30 Preparation of 2,4—bis(1—adamantylamino)—6—(1—piperaziny1)- pyrimidine

The reaction of 2,4—bis(l—adamantylamino)—6—chloropyri- midine with piperazine as described in Example 4 gives the title compound in a yield of 83.36%, m.p.: 168-175°C. '-H-NMR (60 MHz, CDC1 ₃ ) δ ppm: 4.97 (s, IH, 5-H).

Example 31 Preparation of 2—(1-^damantylamino)—4—chloro—6-pyrrolidino- pyrimidine

By reacting 2—(1-adamantylamino)-4,6-dichloropyrimidine with pyrrolidine as described in Example 12, the title com¬ pound is obtained in a yield of 86%, m.p.: 178-180°C. ^l -H-NMR (60 MHz, CDCI ₃ ) δ ppm: 5.62 (s, IH, 5-H). Example 32

Preparation of 2—(1—adamantylamino)—4—(1—piperazinyl)—6—pyr¬ rolidinopyrimidine

By reacting 2—(1—adamantylamino)—4—chloro—6—pyrroli- dinopyrimidine with piperazine as described in Example 4, the title compound is obtained in a yield of 69.7%, m.p.: 160-164°C. ^l -H-NMR (60 MHz, CDCI ₃ ) δ ppm: 4.87 (s, IH, 5-H).

Example 33 Preparation of 4—(1—adamantylamino)—6—chloro—2—pyrrolidino- pyrimidine

4—(1—Adamantylamino)—2,6-dichloropyrimidine is reacted with pyrrolidine as described in Example 12 to obtain the title product in a yield of 80.2%, m.p.: 186-190°C. ^l -H-NMR (60 MHz, CDCI ₃ ) δ ppm: 5.63 (s, IH, 5-H). Example 34

Preparation of 4—(1—adamantylamino)—€—(1-piperazinyl)—2—pyr¬ rolidinopyrimidine

4—(1-Adamantylamino)—6—chloro—2-pyrrolidinopyrimi dine is reacted with piperazine as described in Example 4 to give the title compound in a yield of 49.7%, m.p.: 152-156°C. l-H-NMR (60 MHz, CDCI ₃ ) <S ppm: 4.84 (s, IH, 5-H).

Example 35 Preparation of 4,6—dichloro—2—(2,2,6,6—tetramethy1—1—piperi dinyl)pyrimidine

The mixture of 25 g (136.3 mmoles) of 2,4,6-trichloro pyrimidine and 46.3 ml (272.6 mmoles) of 2,2,6,6-tetra methylpiperidine is boiled under reflux for 50 hours, the cooled down and suspended in 250 ml of hexane. The insolubl material is filtered off, the filtrate is evaporated and th evaporation residue is distributed between 300 ml of chloro form and 50 ml of 10% sodium hydroxide solution. Afte separation the organic phase is washed 4 times with 100 m of water each, then dried and evaporated. The evaporatio residue is purified by chromatography by using hexane a eluent. After recrystallization of the obtained produ. fro hexane, the title compound is obtained in a yield of 8.04

(20.47%), m.p.: 89-90°C. ⁱ H-NMR (60 MHz, CDCl ₃ ) δ ppm: 6.53 (s, IH, 5-H).

Example 36 Preparation of 4-chloro—2—(2,2,6,6—tetramethy1—1—piperidi nyl)—6—pyrrolidinopyrimidine

4,6-Dichloro—2—(2,2,6,6-tetramethyl-l-piperidinyl)pyr i midine is reacted with pyrrolidine as described in Exampl 12 to give the title compound in a yield of 75.08%, m.p.: 130-135°C. ⁱ H-N R (60 MHz, CDCI ₃ ) <5 ppm: 5.76 (s, IH, 5-H).

Example 37 Preparation of 2—(2,2,6,6—tetramethy1—1—piperidinyl)—4—(1 —piperaziny1)—6—pyrro1idinopyrimidine

4-Chloro-2—(2,2,6,6-tetramethy1-1-piperidinyl)-6-pyrro 1idinopyrimidine is reacted with piperazine as described i Example 42 to give the title compound in a yield of 80.2%, m.p.: 134-137°C. ^l -H-NMR (60 MHz, CDCI ₃ ) δ ppm: 5.01 (s, IH, 5-H).

Example 38 Preparation of 2—(4,4—ethylenedioxy—1—piperidinyl)—4,6-di¬ chloropyrimidine and 4—(4,4—ethylenedioxy—1—piperidinyl)- —2,6—dichloropyrimidine After dropwise adding 43.32 g (286 mmoles) of 1,4-di- oxa-8-azaspiro[4,5]decane to a solution of 25 g (136.3 mmoles) of 2,4,6-trichloropyrimidine in 200 ml of tetrahyd¬ rofuran at 0°C, the reaction mixture is stirred at room tem¬ perature for 1 hour, then evaporated. The residue is distri- buted between 300 ml of chloroform and 100 ml of 10% sodium hydroxide solution. After separating the organic phase is washed 4 times with 100 ml of water each, then dried and evaporated. The evaporation residue is subjected to chro¬ matography on a silica gel column by using chloroform as eluent. The less polar 2—(4,4-ethylenedioxy—1-piperidinyl)- —4,6—dichloropyrimidine is recrystallized from ethyl acetate to result in a yield of 13.98 g (35.36%), m.p.: 104-105°C. ^l -H-NMR (60 MHz, CDC1 ₃ ) δ ppm: 6.50 (s, IH, 5-H).

The more polar 4—(4,4-ethylenedioxy—1—piperidinyl)—2,6- —dichloropyrimidine is also recrystallized from ethyl acetate to result in a yield of 20.98 g (53.04%), m.p.: 133- 136°C. ⁱ H-NMR (60 MHz, CDCI ₃ ) <S ppm: 6.40 (s, IH, 5-H). Example 39 Preparation of 2,4—bis(4, — thylenedioxy—1-^piperidinyl)—6- —chloropyrimidine

After adding 2.6 ml (17.23 mmoles) of 1,4—dioxa-8-aza- spiro[4,5]decane to the solution of 2.0 g (6.89 mmoles) of 2—(4,4—ethylenedioxy—1—piperidinyl)—4,6—dichloro pyrimidine in 40 ml of n—butanol, the reaction mixture is boiled under reflux for 4 hours, then evaporated. The evaporation residue is distributed between 50 ml of chloroform and 5 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 10 ml of water each, then dried and evaporated. After recrystallizing the obtained product from hexane the title compound is obtained in a yield of

2.51 g (91%), m.p.: 130-131°C.

^-H-NMR (60 MHz, CDC1 ₃ ) δ ppm: 5.88 (s, IH, 5-H).

Example 40 Preparation of 2,4—bis(4,4—ethylenedioxy—1—piperidinyl)—6- —(1—piperazinyl)pyrimidine

The reaction of 2,4—bis(4,4—ethylenedioxy—1—piperidi¬ nyl)—6—chloropyrimidine with piperazine as described in Example 4 gives the title compound in a yield of 55.7%, m.p.: 130-140°C. ¹ H-NMR (60 MHz, CDCI3) δ ppm: 5.01 (s, IH, 5-H). Example 41 Preparation of 2,4—bis(4,4-ethylenedioxy—1—piperidinyl)—6- —chloropyrimidine and 4,6—bis(4,4—ethylenedioxy—1—piperidi¬ nyl) —2—chloropyrimidine After adding 26 ml (172.3 mmoles) of 1,4— ioxa—8—aza- spiro[4,5]decane to a solution of 20.0 g (68.9) mmoles) of 2—(4,4—ethylenedioxy—1—piperidinyl)—4,6—dichloro pyrimidine in 400 ml of n—butanol, the reaction mixture is boiled under reflux for 4 hours, then evaporated. The evaporation residue is distributed between 500 ml of chloroform and 50 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 100 ml of water each, then dried and evaporated. The evaporation residue is subjected to chromatography by using chloroform as eluent on a silica gel column. The less polar 2,4—bis(4,4—ethylenedioxy—1— ipe- ridinyl)—6—chloropyrimidine is recrystallized from ethyl acetate to give a yield of 16.58 g (73.6%), m.p.: 130-131°C. ^-NM (60 MHz, CDCI ₃ ) δ ppm: 5.87 (s, IH, 5-H).

The more polar 4,6—bis(4,4—ethylenedioxy—1—piperidi- nyl)—2—chloropyrimidine is also recrystallized from ethyl acetate to result in a yield of 2.66 g (11.8%), m.p.: 149- 150°C. ⁱ H-NM (60 MHZ, CDCI ₃ ) δ ppm: 5.48 (s, IH, 5-H).

Example 42 Preparation of 4,6—bis(4, —ethylenedioxy—1-piperidinyl)—2- —(1-piperaziny1)pyrimidine

The reaction of 4,6—bis(4,4-ethylenedioxy—1-piperidi- nyl)—2—chloropyrimidine with piperazine as described in Example 4 gives the title compound in a yield of 58.9%, m.p.: 122-126°C. !H-NMR (60 MHz, CDC1 ₃ ) ^δ PP ^m: - ⁹⁹ ( ^s ' ^1H ' 5-H).

Example 43 Preparation of 2—cyclopentylamino—4,6—dichloropyrimidine and 2,6— ichloro—4—cyclopentylaminopyrimidine

2,4,6-Trichloropyrimidine is reacted with cyclopentyl- amine as described in Example 2 to obtain the less polar 2- —cyclopentylamino-4,6—dichloropyrimidine in a yield of 35.2%, m.p.: 48-52°C.

^H-NMR (60 MHZ, CDCI ₃ ) δ ppm: 6.52 (s, IH, 5-H).

The more polar 2,6-dichloro-4-cyclopentylaminopyrimi- dine is obtained as an oily product in a yield of 57.2%. ^- MR (60 MHz, CDCI3) δ ppm: 6.30 (s, IH, 5-H). Example 44

Preparation of 2—cyclopentylamino—4—chloro—6— yrrolidinopy- rimidine

2—Cyclopentylamino-4,6-dichloropyrimidine is reacted with pyrrolidine as described in Example 12 to give the title compound as an oily product in a yield of 72.4%. ⁱ H-NMR (60 MHz, CDCI3) <S ppm: 5.72 (s, IH, 5-H).

Example 45 Preparation of 2—cyclopentylamino—4—(1-piperazinyl)—6— yrro¬ lidinopyrimidine 2—Cyclopentylamino—4—chloro—6—pyrrolidinopyrimidin e is reacted with piperazine as described in Example 4 to afford the title compound in a yield of 63.8%, m.p.: 125-128°C. -H-NMR (60 MHZ, CDCI ₃ ) δ ppm: 4.84 (s, IH, 5-H).

Example 46 Preparation of 6—chloro—2,4—bis(2,2-dimethylpropylamino)py rimidine and 2-chloro—4,6—bis(2,2—dimethyIpropylamino)pyri midine After dropwise adding 5.00 g (27.26 mmoles) of 2,4,6

—trichloropyrimidine to the solution of 11.88 g (136. mmoles) of l-amino-2,2-dimethyIpropane in 50 ml of isopro panol while cooling and stirring, the reaction mixture i boiled under reflux for 20 hours, then evaporated. The evap oration residue is distributed between 80 ml of chlorofor and 25 ml of 10% sodium hydroxide solution. After separatio the organic phase is washed 4 times with 20 ml of wate each, then dried and evaporated. The evaporation residue i subjected to chromatography by using a mixture of hexane an ethyl acetate as eluent on a silica gel column. By carryin out the elution with a 99:1 mixture, 6—chloro—2,4—bis(2,2 —dimethyIpropylamino)pyrimidine is obtained which is recry stallized from hexane to give a yield of 5.74 g (73.9%) m.p. : 95-97°C. By continuing the elution with a 19:1 mixture o hexane/ethyl σetate, the more polar 2—chloro—4,6—bis(2,2 —dimethyIpropylamino)pyrimidine is obtained which is recrys tallized from hexane to give a yield of 0.16 g (2.8%), m.p. 178-185°C. Example 47

Preparation of 21—(4—bromobenzenesulfonyloxy)—16α— ethyl pregna—1 _f 4,9(11)— riene—3,20— ione

To a solution containing 10.0 g (29.4 mmoles) of 21 —hydroxy—16α-methylpregna-l,4,9(ll)-triene-3,20-dione in 10 mol of tetrahydrofuran, first 7,14 ml (51.4 mmoles) of tri ethylamine, then at 0°C 13.1 g (51.4 mmoles) of 4-bromoben zenesulfonyl chloride are added, the reaction mixture i stirred at room temperature for 4 hours, then it is dropwis added to 450 ml of water while stirring. The precipitate i filtered off, dried and recrystallized from ether to obtai 11.0 g (67.07%) of the title compound, m.p.: 124-129°C.

- 29 -

^l -H-NMR (300 MHZ, CDCl ₃ ) δ ppm: 0.65 (s, 3H, I8-CH ₃ ) , 0.93 (d, IH, I60.-CH ₃ ) , 1.40 (s, 3H, I9-CH ₃ ), 4.54 es 4.66 (d, d, 2H, 21-CH ₂ ), 5.50 (m, IH, 11-H) , 6.07 (t, IH, 4-H) , 6.29 (dd, IH, 2-H) , 7.16 (d, IH, 16H) , 7.72 (d, 2H, phenylene C3-H) , C5-H) , 7.83 (d, 2H, phenylene

C2-H, C6-H) . Example 48 Preparation of 21—{4—[2,4—bis(1—adamantylamino)—6—pyrimidi- nyl]—1-piperazinyl}—16α-methylpregna—1,4,9(11)—trie ne—3,20- -dione

After adding 1.88 g (4.07 mmoles) of 2,4-bis(1—adaman- tylamino)—6-(1—piperazinyl)pyrimidine and 0.56 g of potassium carbonate to a solution containing 2.00 g (3.57 mmoles) of 21—(4-bromobenzenesulfonyloxy)-16α-methylpregna- —1,4,9(11)—triene—3,20—dione in 100 ml of acetonitrile, the reaction mixture is stirred at 65°C for 5 hours, then evap¬ orated. The residue is distributed between 40 ml of chloro¬ form and 10 ml of water. After separation the chloroform solution is dried, evaporated and the residue is purified by chromatography on a silica gel column. A 98:2 mixture of chloroform/methanol is used for elution and the product obtained is recrystallized from ether to give the title com¬ pound in a yield of 2.48 g (88.5%), m.p.: 210-220°C. ^-NMR (250 MHz, CDCI ₃ ) ^s PP ^m: °- ⁶⁸ ( ^s , ^3H » I8-CH ₃ ) , 0.96 (d, IH, I60.-CH ₃ ), 1.40 (s, 3H, 19-CH ₃ ) , 3.13 and 3.23

(d, d, 2H, 21-CH ₂ ), 4.98 (s, IH, pyrimidine, C5-H) , 5.51 (m, IH, 11-H), 6.07 (m, IH, 4-H), 6.28 (d, d, IH, 2-H), 7.16 (d, IH, 1-H) . Example 49 Preparation of 2,4—bis(1—adamantylamino)—6—chloropyrimidin and 4,6—bis(1— damantylamino) —2—chloropyrimidine

26.0 g (87.25 mmoles) of 4—(1-adamantylamino)—2,6—di¬ chloropyrimidine and 39.5 g (261.6 mmoles) of 1—aminoadaman¬ tane are dissolved in 200 ml of n—butanol, then the reac- tion mixture is boiled for 75 hours and evaporated. The residue is suspended in 400 ml of ether and filtered. The

recovered material is chromatographed after drying on a silica gel column by using chloroform as eluent. The obtained substance is a mixture of the title isomers. These are separated on a silica gel column by using a mixture of hexane and ethyl acetate. By using a 49:1 mixture of these solvents as eluent 2,4—bis(1—adamantylamino)—6—chloropyrimi¬ dine is obtained which is recrystallized to obtain a yield of 21.67 g (60.14%) .

By continuing the elution with a 6:1 mixture of said solvents the more polar 4,6—bis(1—adamantylamino)—2—chloro¬ pyrimidine is obtained which is recrystallized from hexane to obtain a yield of 1.88 g (5.22%), melting point: 260- 266°C. ³ -H-NMR (250 MHz, CDC1 ₃ ) δ ppm: 5.49 (s, IH, 5-H). Example 50

Preparation of 4,6—bis(1— damantylamino)—2—(1—piperazinyl)- pyrimidine

4,6—Bis(1—adamantylamino)—2—chloropyrimidine is reacted with piperazine as described in Example 4 to obtain the title compound in a yield of 94.4%, m.p.: 210-220°C. -H-NMR (60 MHz, CDCI ₃ ) δ ppm: 4.97 (S, IH, 5-H).

Example 51 Preparation of 2,4—bis(cyclopentylamino)—6—chloropyrimidine 5.0 g of 2—(cyclopentylamino)—4,6-dichloropyrimidine are dissolved in 25 ml of isopropanol, then 7.5 ml of cyclo- pentylamine are added and the mixture is boiled for 6 hours. Then the reaction mixture is evaporated, the residue is separated between 80 ml of chloroform and 15 ml of 10% sodium hydroxide solution. After separation the organic phase is washed 4 times with 20 ml of water each, dried and evaporated. The title compound is obtained by recrys- tallizing from hexane in a yield of 5.24 g (86.7%), m.p.: 94-98°C. iH-NMR (60 MHz, CDCI ₃ ) δ ppm: 5.67 (s, IH, 5-H).

Example 52 Preparation of 2,4—bis(cyclopentylamino)—6—(1-piperazinyl)- pyrimidine

2,4—Bis(cyclopentylamino)—6—chloropyrimidine is reacted with piperazine as described in Example 4 to give the title compound in a yield of 81.9%, m.p.: 142-148°C. ^X H-NMR (60 MHZ, CDC1 ₃ ) ^δ PP ^{m: 4} - ⁹⁴ ( ^s ' ^1H ' 5-H).