Title:
NOVEL PROTEIN COMPLEX, METHOD FOR MATURATION OF COBALT-TYPE LOW-MOLECULAR-WEIGHT NITRILE HYDRATASE USING THE PROTEIN COMPLEX, MATURED COBALT-TYPE LOW-MOLECULAR-WEIGHT NITRILE HYDRATASE, AND METHOD USING THE NITRILE HYDRATASE
Document Type and Number:
WIPO Patent Application WO/2008/004473
Kind Code:
A1
Abstract:
[PROBLEMS] To provide a novel protein complex involved in the maturation of a cobalt-type low-molecular-weight nitrile hydratase. [MEANS FOR SOLVING PROBLEMS] A trimeric protein complex αe2 formed by an NhlA protein molecule having the amino acid sequence depicted in SEQ ID NO:1 (α-subunit of cobalt-type low-molecular-weight nitrile hydratase) and two NhlE protein molecules each having the amino acid sequence depicted in SEQ ID NO:2. The protein complex can be used for maturing a cobalt-type low-molecular-weight nitrile hydratase which has been purified in an immature state. The matured cobalt-type low-molecular-weight nitrile hydratase is highly reactive, and therefore can be used for the production of an amide compound on an industrial scale.
Inventors:
KOBAYASHI MICHIHIKO (JP)
HASHIMOTO YOSHITERU (JP)
SHIRAKI KENTARO (JP)
HASHIMOTO YOSHITERU (JP)
SHIRAKI KENTARO (JP)
Application Number:
PCT/JP2007/062872
Publication Date:
January 10, 2008
Filing Date:
June 27, 2007
Export Citation:
Assignee:
UNIV TSUKUBA (JP)
KOBAYASHI MICHIHIKO (JP)
HASHIMOTO YOSHITERU (JP)
SHIRAKI KENTARO (JP)
KOBAYASHI MICHIHIKO (JP)
HASHIMOTO YOSHITERU (JP)
SHIRAKI KENTARO (JP)
International Classes:
C12N15/09; C07K14/195; C12N9/88; C12P13/02
Foreign References:
| JPH04211379A | 1992-08-03 |
Other References:
KOBAYASHI M. ET AL.: "Cloning, nucleotide sequence and expression in Escherichia coli of two cobalt-containing nitrile hydratase genes from Rhodococcus rhodochrous J1", BIOCHIM. BIOPHYS. ACTA, vol. 1129, 1991, pages 23 - 33, XP000653047
KOMEDA H. ET AL.: "A novel transporter involved in cobalt uptake", PROC. NATL. ACAD. SCI. U.S.A., vol. 94, 1997, pages 36 - 41, XP009050024
DATABASE GENBANK [online] KOMEDA H. ET AL., XP003020624, accession no. EMBL Database accession no. (D83695)
KOMEDA H. ET AL.: "A novel transporter involved in cobalt uptake", PROC. NATL. ACAD. SCI. U.S.A., vol. 94, 1997, pages 36 - 41, XP009050024
DATABASE GENBANK [online] KOMEDA H. ET AL., XP003020624, accession no. EMBL Database accession no. (D83695)
Attorney, Agent or Firm:
WATANABE, Kaoru (SUCCESS-SENGAKUJI BLDG 3F2-20-29, Takanaw, Minato-ku Tokyo 74, JP)
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