Title:
NOVEL PROTEIN, GENE ENCODING THE SAME AND METHOD OF USING THE SAME
Document Type and Number:
WIPO Patent Application WO/2002/072817
Kind Code:
A1
Abstract:
It is intended to search for and identify a novel antimicrobial protein whereby the growth of pathogenic bacteria for plants (for example, Pyricularia orizae and Rhizoctonia solani causing two major diseases of rice plant) at a relatively low concentration and to clone the gene of this protein. Thus, a novel antimicrobial protein which can be obtained from a fraction obtained from a water extract of Pleurotus cornucopiae by the ammonium sulfate precipitation method, has an antimicrobial activity at least against P. orizae and R. solani, and shows the presence of an about 15 kDa component in SDS-PAGE is provided.
Inventors:
TAKAKURA YOSHIMITSU (JP)
Application Number:
PCT/JP2002/002295
Publication Date:
September 19, 2002
Filing Date:
March 12, 2002
Export Citation:
Assignee:
JAPAN TOBACCO INC (JP)
SYNGENTA LTD (GB)
TAKAKURA YOSHIMITSU (JP)
SYNGENTA LTD (GB)
TAKAKURA YOSHIMITSU (JP)
International Classes:
A01N63/50; A01N65/00; C07K14/375; C12N1/15; C12N1/19; C12N1/21; C12N15/82; (IPC1-7): C12N15/09; A01H5/00; A01N37/46; A01N63/00; A01N65/00; C07K14/375; C12N1/15; C12N1/19; C12N1/21; C12N5/10; C12P21/02; C12Q1/68
Domestic Patent References:
| WO1989003422A1 | 1989-04-20 |
Foreign References:
| JPH07289264A | 1995-11-07 |
Other References:
EDWARD A. BAYER ET AL.: "Close similarity among streptavidan-like, biotin-binding proteins from streptomyces", BIOCHIMICA ET BIOPHYSICA ACTA, vol. 1263, 1995, pages 60 - 66, XP002951808
CARLOS E. ARGARANA ET AL.: "Molecular cloning and nucleotide sequence of the streptavidin gene", NUCLEIC ACIDS RESEARCH, vol. 14, no. 4, 1986, pages 1871 - 1882, XP002951809
SAMBROOK ET AL.,: "Molecular Cloning, 2nd edition", 1989
CARLOS E. ARGARANA ET AL.: "Molecular cloning and nucleotide sequence of the streptavidin gene", NUCLEIC ACIDS RESEARCH, vol. 14, no. 4, 1986, pages 1871 - 1882, XP002951809
SAMBROOK ET AL.,: "Molecular Cloning, 2nd edition", 1989
Attorney, Agent or Firm:
Shamoto, Ichio (Section 206 New Ohtemachi Bldg., 2-1,
Ohtemachi 2-chom, Chiyoda-ku Tokyo, JP)
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