Title:
NUCLEIC ACID APTAMER TEST STRIP, PREPARATION METHOD THEREFOR, AND USE THEREOF
Document Type and Number:
WIPO Patent Application WO/2024/083104
Kind Code:
A1
Abstract:
A nucleic acid aptamer test strip, a preparation method therefor, and the use thereof, belonging to the technical field of pathogenic bacterium detection. The nucleic acid aptamer test strip comprises: a sample pad (1), a conjugate pad (2), a nitrocellulose membrane (3), a water absorbent pad (6) and a base plate (7). The conjugate pad (2) contains one or multiple capture aptamers. The nitrocellulose membrane (3) contains one or multiple test lines (4) and a control line (5), the test line (4) containing a test aptamer, and the control line (5) containing a control aptamer. The capture aptamer on the conjugate pad (2) is modified on silver-core gold-shell nanoparticles. The nucleic acid aptamer test strip uses a nucleic acid aptamer in replacement of an antibody, uses a sandwich assay to capture pathogenic bacteria, and further uses the silver-core gold-shell nanoparticles as a signal amplification element in replacement of colloidal gold used by traditional test strips, thus raising the detection sensitivity, lowering the detection cost, and achieving easy operation. Further, the nucleic acid aptamer test strip allows naked eye detection and observation, has high stability, and is suitable for on-site rapid detection.
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Inventors:
WU WEI (CN)
WANG QINZHI (CN)
HOU XIUDAN (CN)
LV LIANGTAO (CN)
YANG QINGLI (CN)
ZHAO FANGYUAN (CN)
WANG QINZHI (CN)
HOU XIUDAN (CN)
LV LIANGTAO (CN)
YANG QINGLI (CN)
ZHAO FANGYUAN (CN)
Application Number:
PCT/CN2023/124890
Publication Date:
April 25, 2024
Filing Date:
October 17, 2023
Export Citation:
Assignee:
UNIV QINGDAO AGRICULTURAL (CN)
International Classes:
G01N33/569; G01N33/558
Foreign References:
| CN116008542A | 2023-04-25 | |||
| CN109596827A | 2019-04-09 | |||
| CN106568951A | 2017-04-19 | |||
| CN105116146A | 2015-12-02 | |||
| CN106198967A | 2016-12-07 | |||
| CN106636387A | 2017-05-10 | |||
| CN209784370U | 2019-12-13 | |||
| CN113267628A | 2021-08-17 | |||
| CN113607790A | 2021-11-05 |
Other References:
HAI‐BIN LIU: "Functionalized Au MBA @Ag Nanoparticles as an Optical and SERS Dual Probe in a Lateral Flow Strip for the Quantitative Detection of Escherichia coli O157:H7", JOURNAL OF FOOD SCIENCE, WILEY-BLACKWELL PUBLISHING, INC, US, vol. 84, no. 10, 1 October 2019 (2019-10-01), US , pages 2916 - 2924, XP093159890, ISSN: 0022-1147, DOI: 10.1111/1750-3841.14766
LU CHUNXIA: "Research on spatial distribution of livestock and poultry pollutant in Guangzhou City", JOURNAL OF FOOD SCIENCE AND BIOTECHNOLOGY, vol. 39, no. 4, 15 April 2020 (2020-04-15), pages 85 - 92, XP093159906, DOI: 10.3969/j.issn
LU CHUNXIA: "Research on spatial distribution of livestock and poultry pollutant in Guangzhou City", JOURNAL OF FOOD SCIENCE AND BIOTECHNOLOGY, vol. 39, no. 4, 15 April 2020 (2020-04-15), pages 85 - 92, XP093159906, DOI: 10.3969/j.issn
Attorney, Agent or Firm:
SIMBA IP LAW FIRM (CN)
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