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Title:
NUCLEIC ACID DETECTION CASSETTE
Document Type and Number:
WIPO Patent Application WO/2016/035817
Kind Code:
A1
Abstract:
In this nucleic acid detection cassette, a designated syringe is squeezed such that a sample solution is provided via a channel to an amplification unit, and the amplification unit is heated by an external unit such that sample DNA in the sample solution is amplified. The sample solution containing the amplification product is provided via a channel to a detection unit, where a hybridization reaction occurs. Next, a separate syringe is squeezed such that a liquid containing an intercalating agent is provided to the detection unit via a separate designated channel. As a result, a nucleic acid detection cassette that can automate nucleic acid detection of a sample from amplification to detection by electrochemical reaction is provided.

Inventors:
OKADA JUN (JP)
YUMOTO MASAYUKI (JP)
ARAME KENICHI (JP)
KUWABARA TETSUYA (JP)
KUSHIMA HIROAKI (JP)
UNNO HIROTAKA (JP)
Application Number:
PCT/JP2015/074948
Publication Date:
March 10, 2016
Filing Date:
September 02, 2015
Export Citation:
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Assignee:
TOSHIBA KK (JP)
International Classes:
C12M1/00; C12M1/34; C12N15/09; C12Q1/68
Domestic Patent References:
WO2010050208A12010-05-06
WO2013166857A12013-11-14
Foreign References:
JP2004212361A2004-07-29
JP2001235468A2001-08-31
JP2005176836A2005-07-07
JP2013051958A2013-03-21
JP2008261816A2008-10-30
JP2005261298A2005-09-29
JP2013066463A2013-04-18
JP2013198443A2013-10-03
JP2014062870A2014-04-10
JP2009109334A2009-05-21
JP2010075072A2010-04-08
Other References:
MASAYOSHI TAKAHASHI: "Denryu Kenshutsugata DNA Chip o Mochiita Shokuchudoku Gen'inkin no Kan'i Jido Kensa Gijutsu no Kaihatsu", JAPANESE SOCIETY OF FOOD MICROBIOLOGY GAKUJUTSU SOKAI KOEN YOSHISHU, vol. 33, 2012, pages 30, XP009500870
See also references of EP 3190169A4
Attorney, Agent or Firm:
S & S International PPC (JP)
Patent business corporation スズエ international patent firm (JP)
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